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<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T0</accession>
    <name>F0T5T0_METLA</name>
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        <submittedName>
            <fullName evidence="3">Conserved repeat domain protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="ordered locus">Metbo_1078</name>
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    <organism evidence="3 4">
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_013644674.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1078"/>
    <dbReference type="EnsemblBacteria" id="ADZ09323">
        <property type="protein sequence ID" value="ADZ09323"/>
        <property type="gene ID" value="Metbo_1078"/>
    </dbReference>
    <dbReference type="GeneID" id="10277527"/>
    <dbReference type="KEGG" id="mel:Metbo_1078"/>
    <dbReference type="eggNOG" id="arCOG02487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313006_0_0_2"/>
    <dbReference type="OMA" id="NTTFKVM"/>
    <dbReference type="OrthoDB" id="7at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032109">
        <property type="entry name" value="Big_3_5"/>
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    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16640">
        <property type="entry name" value="Big_3_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="SSF49447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_3_5" evidence="2">
        <location>
            <begin position="283"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Big_3_5" evidence="2">
        <location>
            <begin position="473"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Big_3_5" evidence="2">
        <location>
            <begin position="569"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="750"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="869" mass="92820" checksum="A25EF1BD928B993A" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U2</accession>
    <name>F0T5U2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644686.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09335">
        <property type="protein sequence ID" value="ADZ09335"/>
        <property type="gene ID" value="Metbo_1090"/>
    </dbReference>
    <dbReference type="GeneID" id="10277539"/>
    <dbReference type="KEGG" id="mel:Metbo_1090"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_0_3_2"/>
    <dbReference type="OMA" id="WRTLTAY"/>
    <dbReference type="OrthoDB" id="62989at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="36700" checksum="B52C2D1585B9BDE1" modified="2011-05-03" version="1">MFEDIKIPGYLNKIVVLVLVILALAGLKLVSSILGPILIAMFFSILIYPFLKWLNRRGFSYNVSIIITLVGFAVLGGVIIEILYFSLSQMVSALPSFSINSSSALSSEANYVAQTIISHFPMGAVTDIVGTAVFVFFATIFMIYEIPNLKVRLEKGLGKDNNNLNEMIGLIDTFIKYFLIRVKVNLFAALGFIGIFLAFDINFALLWGILTFFLGFIPYLGIMIAAIPPILATWAKYGIQGAILITILFIIVNTIAESIIFPRQTGKGLQMSIYVVFVSLFVWGWILGPSGFLLAIPLTIIVIKYLEKYDETRWLAILMTSDDDENNVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y4</accession>
    <name>F0T5Y4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN_Apea domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644728.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09377">
        <property type="protein sequence ID" value="ADZ09377"/>
        <property type="gene ID" value="Metbo_1133"/>
    </dbReference>
    <dbReference type="GeneID" id="10277583"/>
    <dbReference type="KEGG" id="mel:Metbo_1133"/>
    <dbReference type="eggNOG" id="arCOG09531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777580_0_0_2"/>
    <dbReference type="OrthoDB" id="129351at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041229">
        <property type="entry name" value="HEPN_Apea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18739">
        <property type="entry name" value="HEPN_Apea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN_Apea" evidence="1">
        <location>
            <begin position="205"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="356" mass="43053" checksum="0868B238F058732E" modified="2011-05-03" version="1">MDLKLNNIKHNSKKNFKFIFLLNIKRFRLIDEDDSDYKNERKIYENVLKIFDIERVVIEKFTIDGAYEDQSNLNKLMQIFKNSHGETIEISINARDFEFAEKLANSKLDLFLGFFSFMRYRFLKRIDFNDDKPIDKINAKSCIVLEGDKLIYPYLKYVPERTLDSIRNEVNEVRFTNSIDLKIRYNLSYFEHYIELMKETKNDKSKKKLWKCLTEFLRLYYDACTEKNLPYAFLKFWMIGETIIKKISGKIKDENLLKRLEQVFKVFHNDKFIRNRFKFLYLRRNELVHEGNFEQITYVDRNLAKIIADSVLLFYISYMNDLNNLAEYDFLIQNMNKSDNEIDRYSLVLKIISKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T615</accession>
    <name>F0T615_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10522">
        <property type="protein sequence ID" value="ADZ10522"/>
        <property type="gene ID" value="Metbo_2308"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2308"/>
    <dbReference type="eggNOG" id="arCOG14543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067831_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="14104" checksum="95402663703DE9D1" modified="2011-05-03" version="1">MVRSLDPHIKNFDVVKYRTNLYKHLQKIDEIEYYNSKIRKTTSKPKRTTGNNFKSVEDQRILFRQILGVPVHEYPEKEYVMYNCPFHDHEDKNLLLALIKMDITVTDVVEKVIIGNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T664</accession>
    <name>F0T664_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber evidence="5 12">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">guaAB</name>
        <name evidence="15" type="ordered locus">Metbo_2358</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="5 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645922.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2358"/>
    <dbReference type="EnsemblBacteria" id="ADZ10571">
        <property type="protein sequence ID" value="ADZ10571"/>
        <property type="gene ID" value="Metbo_2358"/>
    </dbReference>
    <dbReference type="GeneID" id="10278824"/>
    <dbReference type="KEGG" id="mel:Metbo_2358"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OMA" id="EGGIKSH"/>
    <dbReference type="OrthoDB" id="31932at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ10571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="308" mass="34009" checksum="DCBF971B5851035B" modified="2011-05-03" version="1">MLNPSDFIEESIKDIKKTIGDGKAIIALSGGVDSSVASVLTSTAIGENLVAVFVDHGLLREGESDYVNNTFKDRLNLRTVDASEEFLSRLDGVYDPEEKRKIIGEVFIRVFERVAEEVGAEFLVQGTIAPDWIESEGQIKSHHNIALPHGLVLNIVEPIRELYKDEVRVVGTELGLPEEMVNRQPYPGPGLAVRIVGKLTEEKIEICRKANAIVEEEVKKEGLDASLWQYFAVLTDTKVTGVKGDIRDYGYLVVLRMVESIDAMTASVPELPWSMVKTISQRITAEVSEVTHVSLSVSDKPPSTIEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E4</accession>
    <name>F0T6E4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644810.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1216"/>
    <dbReference type="EnsemblBacteria" id="ADZ09459">
        <property type="protein sequence ID" value="ADZ09459"/>
        <property type="gene ID" value="Metbo_1216"/>
    </dbReference>
    <dbReference type="GeneID" id="24964541"/>
    <dbReference type="KEGG" id="mel:Metbo_1216"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362753_0_0_2"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="454"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="770" mass="91476" checksum="3B7C94CAE0998BE4" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E5</accession>
    <name>F0T6E5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644811.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09460">
        <property type="protein sequence ID" value="ADZ09460"/>
        <property type="gene ID" value="Metbo_1218"/>
    </dbReference>
    <dbReference type="GeneID" id="10277669"/>
    <dbReference type="KEGG" id="mel:Metbo_1218"/>
    <dbReference type="HOGENOM" id="CLU_1773201_0_0_2"/>
    <dbReference type="OrthoDB" id="138503at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16431" checksum="98564E5584CBDEF7" modified="2011-05-03" version="1" precursor="true">MKKNMVPIIVLIIIIALFAGSVSATNNTNGVSIQKVVQKNLIDSGSNKFYWGERGGLFAYTWKTYKTSNGVVVQVHYKTINNSWYSTYSITKLTSSKLKIVETSPEVKLYSGRSSVTSYAKTSLTPVTYYWKNIRPQIKSDGYFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F2</accession>
    <name>F0T6F2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645937.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2373"/>
    <dbReference type="EnsemblBacteria" id="ADZ10586">
        <property type="protein sequence ID" value="ADZ10586"/>
        <property type="gene ID" value="Metbo_2373"/>
    </dbReference>
    <dbReference type="GeneID" id="10278839"/>
    <dbReference type="KEGG" id="mel:Metbo_2373"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="OMA" id="DPLAVWK"/>
    <dbReference type="OrthoDB" id="80501at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039912">
        <property type="entry name" value="PNO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="PTHR12826:SF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="85"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="192" mass="21568" checksum="B0D32932A5D74B64" modified="2011-05-03" version="1">MPNTEYLKIPRERVGVLIGKNGITKDEIENLTKTDINIDSETGSISVSPTEATEDPLAVWKSRYIVKAIGRGFNPEISLKLLSDETLLEIINLPDYVGKSKKAIMRQKARIIGKEGRTKDIIIDMTGVDISIYGKTVAIIGGMEQIHIAKEAVEMILNGVRHKTVYAFLERKSRDMKIQEFHRIAKDETDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G8</accession>
    <name>F0T6G8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Elongation factor 1-alpha</fullName>
            <shortName evidence="6">EF-1-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Elongation factor Tu</fullName>
            <shortName evidence="6">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tuf</name>
        <name evidence="8" type="ordered locus">Metbo_2389</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645953.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2389"/>
    <dbReference type="EnsemblBacteria" id="ADZ10602">
        <property type="protein sequence ID" value="ADZ10602"/>
        <property type="gene ID" value="Metbo_2389"/>
    </dbReference>
    <dbReference type="GeneID" id="10278856"/>
    <dbReference type="KEGG" id="mel:Metbo_2389"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="OrthoDB" id="13117at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="413" mass="45283" checksum="5BE4D89E9F601401" modified="2011-05-03" version="1">MAKQKEHMNLAFIGHVDHGKSTLVGHILLQSGAIAEQQLSDGENKFRFVMDKLQEERERGVTIDLAHAKFETPKYEFTIVDCPGHRDFVKNMITGASQADAAVLVVAIDDGVMPQTKEHAFLARTLGINQLIVAINKMDLVKYDEAKFNELKEEVSALIKTVAYKPSEINFIPISAFQGDNITKKSENTPWYKGPALVESLAELKAPEKPTQLPLRVPIQDVYSITGVGTVPVGRVETGIMKKGDNVIFEPPGSSGEVKTIEMHHEMLDQAEPGDNVGFNVRGVGKNDIRRGDVAGHTTNAPTVAKEFTAQIVVLQHPGVITVGYTPVFHCHTAQVACTFMELQKKLDPATGQTKEENPDFLKTGDAAFVVVRPTKPMVIEKIKEIPHMGRFAIRDMGQTVAAGMCIDIVPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K1</accession>
    <name>F0T6K1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2422"/>
    <dbReference type="TCDB" id="1.E.43.2.7">
        <property type="family name" value="the putative transglycosylase-associated holin (t-a hol) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10635">
        <property type="protein sequence ID" value="ADZ10635"/>
        <property type="gene ID" value="Metbo_2422"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2422"/>
    <dbReference type="eggNOG" id="arCOG10440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163807_0_0_2"/>
    <dbReference type="OMA" id="IVWILTI"/>
    <dbReference type="OrthoDB" id="143322at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="12010" checksum="70D82E79B08BA39D" modified="2011-05-03" version="1">MIGLVNWTAAFVGFILAVILSNVFGYFMGFVGISIGLFIAGIVVGLMVGGGVLTGFGNGLVAGAFGAIVISIILLIGGTITAGLVGFGVAAAASIVWILTIFVTSGFVVGVGGAIGSLISGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K3</accession>
    <name>F0T6K3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sortase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645988.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2424"/>
    <dbReference type="EnsemblBacteria" id="ADZ10637">
        <property type="protein sequence ID" value="ADZ10637"/>
        <property type="gene ID" value="Metbo_2424"/>
    </dbReference>
    <dbReference type="GeneID" id="10278896"/>
    <dbReference type="KEGG" id="mel:Metbo_2424"/>
    <dbReference type="eggNOG" id="arCOG07610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096728_0_0_2"/>
    <dbReference type="OMA" id="ITCHPLG"/>
    <dbReference type="OrthoDB" id="62731at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="26473" checksum="A75F756E52719173" modified="2011-05-03" version="1">MKLSTLFVLIGIVIISLYCLIEVSYYSSSDNSSQNSTNTPYIEIPSINVDQAINNKSVDYGIYYDPQSAKPSFGTTALFGHRTFHGSPFLNLDKLKTGDNITVAWPGIGNVEYAVVNSTVVDDSYRLNIEQGNKLFLITCYPLGSDAQRLIIEANQVSIYPINNSQPHSTEPPYGIFIVVGFFVGGMILSFLYPIKDDQYIIFLVVVAITAFLVLAYLFPIPPDAIESQLSWFNNLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K7</accession>
    <name>F0T6K7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative archaetidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="3">4.1.1.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="3">Archaetidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="3">Archaetidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="3" type="primary">asd</name>
        <name evidence="5" type="ordered locus">Metbo_2428</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of archaetidylethanolamine (PtdEtn) from archaetidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>archaetidylserine + H(+) = archaetidylethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:51488"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:134176"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="3">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="3">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645992.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2428"/>
    <dbReference type="EnsemblBacteria" id="ADZ10641">
        <property type="protein sequence ID" value="ADZ10641"/>
        <property type="gene ID" value="Metbo_2428"/>
    </dbReference>
    <dbReference type="GeneID" id="10278900"/>
    <dbReference type="KEGG" id="mel:Metbo_2428"/>
    <dbReference type="eggNOG" id="arCOG04470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_0_0_2"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="OrthoDB" id="105037at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023355085" description="Archaetidylserine decarboxylase beta chain" evidence="3">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023355084" description="Archaetidylserine decarboxylase alpha chain" evidence="3">
        <location>
            <begin position="189"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="3">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25289" checksum="E9864989C3752777" modified="2011-05-03" version="1">MFVKGLSKKKIGILLTIAVLPFLFGYFIISFIIFSIIAFFMQFFRDPQRKIPDGDGVVVAPADGKVLQGKIDFLRIVTVQDDPVMGKILKDGEKGILISTFMSPFDVHVQRAPVSGKILETKYYPGKFKIAMGDVHTVNEKNLIVIDSEYGKVGVIQIAGFVARRIVQYVQPGDTVTIGDRLGMIRFGSRVNLIIPYDKFRVMVFEGEKPTAGETIMAMFNSELNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L2</accession>
    <name>F0T6L2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Biopolymer transport protein ExbD/TolR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2433</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645997.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2433"/>
    <dbReference type="EnsemblBacteria" id="ADZ10646">
        <property type="protein sequence ID" value="ADZ10646"/>
        <property type="gene ID" value="Metbo_2433"/>
    </dbReference>
    <dbReference type="GeneID" id="10278905"/>
    <dbReference type="KEGG" id="mel:Metbo_2433"/>
    <dbReference type="eggNOG" id="arCOG06483">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954707_0_0_2"/>
    <dbReference type="OMA" id="TGPSEYY"/>
    <dbReference type="OrthoDB" id="112006at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="13779" checksum="4925490BF0F97DE9" modified="2011-05-03" version="1">MVLDTSRYRNKLKQSSPQVNLVPLIDVIFTILIFLMVAASFQGSADVDSGKPQINQTTGPSDYYLIPVAGLKTVTVNGVDMSKDIRNGAIAVHTSVIDQGDINIKAKEGAIVITAPAGISPEQAVNVPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N1</accession>
    <name>F0T6N1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="4">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truD</name>
        <name evidence="6" type="ordered locus">Metbo_0011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643615.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0011"/>
    <dbReference type="EnsemblBacteria" id="ADZ08264">
        <property type="protein sequence ID" value="ADZ08264"/>
        <property type="gene ID" value="Metbo_0011"/>
    </dbReference>
    <dbReference type="GeneID" id="10276433"/>
    <dbReference type="KEGG" id="mel:Metbo_0011"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OMA" id="GFPNYFD"/>
    <dbReference type="OrthoDB" id="92795at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="157"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="417" mass="47943" checksum="EBE362517F151DC8" modified="2011-05-03" version="1">MLNAETYETNNKGIGGSIRNEREDFYVEEIPLSLPSGNGPNTWIFIEKIGRNTLDVVLDIAKELKISRKRMGFAGMKDKTARTRQWLCVSNSSVEEIQSITDKLYNVEVLDIKQNEKKLRIGQLVGNKFKILIRNTDDPENDVETAQDVLKQLIETGVPNYYGWQRFGKERSNTHVVGEYLVHNDVKGAVDAYIGNPYPKEREHIKEARSLYDEGKLEEAYQTMPGSMRYEKMLLRELLNLQRRKGELTEKSYIIAIESLPKPLKRMFVHAYQSYLFNKAVSERAKLGLNKYVEGDILIDNEEHLVHEFDVETVEDRIKNFEIHPTSPLYGTKVPLAGGVVGKLEQQVMDEENIKLEEFECPKTPRLGSHGLRRAMRFKIWDVSAEMTPEGVLTEFSIPKGCYATAVLREIMKEDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P4</accession>
    <name>F0T6P4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643628.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0024"/>
    <dbReference type="EnsemblBacteria" id="ADZ08277">
        <property type="protein sequence ID" value="ADZ08277"/>
        <property type="gene ID" value="Metbo_0024"/>
    </dbReference>
    <dbReference type="GeneID" id="10276446"/>
    <dbReference type="KEGG" id="mel:Metbo_0024"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OMA" id="QIDCACA"/>
    <dbReference type="OrthoDB" id="51913at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="MMR_HSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="MMR_HSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="2"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="395" mass="43908" checksum="61A9E88A72D7101C" modified="2011-05-03" version="1">MLQIAVTGKPNVGKSSFFNSATLSEVDVASYPFTTIDANKAIAHVVTDCPCKELELTCNPHNSKCDGGKRLIPVELIDVAGLVPGAHEGRGLGNKFLDDLRQARAFIHIIDASGSTDEEGRPCEPGTHDPLEDVEFLEHEITMWLFGILKKNWNKMIRKVMSERLDIAKVLSEQLSGTGIALEDIAEAKKVVDKDYDKWEDDDIINMLQDLLRRAKPMLIVANKADLPTSEKNIKRLEEKYGRVIPASAESELALIRASEAGLIDYFPGDSNFEVVNPEKLNENQMKALNYIKEHVLQKYGSTGVQEALNAAVFDVLDMIVVFPVEDEHKMSDQKGNVLPDALLIKNGSKPRDMAYVVHTDIGDSFMHAIDARSCRRISSEYEIKDGDILSIICR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R6</accession>
    <name>F0T6R6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="3">DHBP synthase</shortName>
            <ecNumber evidence="3">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643650.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0046"/>
    <dbReference type="EnsemblBacteria" id="ADZ08299">
        <property type="protein sequence ID" value="ADZ08299"/>
        <property type="gene ID" value="Metbo_0046"/>
    </dbReference>
    <dbReference type="GeneID" id="10276469"/>
    <dbReference type="KEGG" id="mel:Metbo_0046"/>
    <dbReference type="eggNOG" id="arCOG01320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="OMA" id="DAGGLIC"/>
    <dbReference type="OrthoDB" id="72536at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25126" checksum="C98302A7F8A5B643" modified="2011-05-03" version="1">MIEKALEALKNGEIVLVFDSDNRERETDMIVAAEFMTPEHMTQIRNDAGGLFCVPLSSENSDALGVPFMTDIMDIASSEYPVLAELNPDDIPYDEKSAFSITVNHRKTFTGITDNDRACTINELASLCKEGKQREFGKYFRAPGHVTLLRAAKGHVRSRKGHTEMSIALMEMAGLTEVAVCCEMMDDKTGGSLPTAEAEQYAKEHDLVFLSGADLIEAYKNYIENDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T0</accession>
    <name>F0T6T0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Daunorubicin resistance ABC transporter ATPase subunit</fullName>
            <ecNumber evidence="7">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643664.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0060"/>
    <dbReference type="EnsemblBacteria" id="ADZ08313">
        <property type="protein sequence ID" value="ADZ08313"/>
        <property type="gene ID" value="Metbo_0060"/>
    </dbReference>
    <dbReference type="GeneID" id="10276483"/>
    <dbReference type="KEGG" id="mel:Metbo_0060"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="SHDMYEV"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="5"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="364" mass="40572" checksum="6D25ABA1392DBF5D" modified="2011-05-03" version="1">MKYAIETFDLSKKYNNDFLAVDELNMTIDNKSIFGFLGPNGAGKTTTIKMLTCLIQPTGGSANVAGFDISKQPNEVREKIGMVPQLVSLYGDLTVRENIELCADFYGLPTDLKISRSEELMELVDIKYAENKLVKQLSGGMKQKASVVASLIHQPDILFLDEPTIGLDPTTKRVLWDLVEELNSEGRTIILCSHDMYEVELLCDKIGIINGGVLAAYDTPRGLKDTMIHQQKEVRSSESSNVIKIMDELRKESDAEDNAAYDQLKEKIERENRDLKELSVMISNLDENMTDHLKNLPEVDLLDEHDSGRIVMDIDSKDESVSKIITEIINKGGIITSISTKDPSLEDVFMSVTSKNKEEGAEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U5</accession>
    <name>F0T6U5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644816.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1223"/>
    <dbReference type="EnsemblBacteria" id="ADZ09465">
        <property type="protein sequence ID" value="ADZ09465"/>
        <property type="gene ID" value="Metbo_1223"/>
    </dbReference>
    <dbReference type="GeneID" id="10277674"/>
    <dbReference type="KEGG" id="mel:Metbo_1223"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_11_2"/>
    <dbReference type="OMA" id="SHMENDI"/>
    <dbReference type="OrthoDB" id="35919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14846" checksum="7174A5B0D99AA401" modified="2011-05-03" version="1">MGNTKIFITEDQMILKLDLKNRLETLNYEVVGMADNGYDAIEQIEKLDPDVVLMDIVLKGELNGIETAQLIKDKFNTPIIYMSAYYDDEILDKAAKTQPDGYITKPYEDVLIRTTIEMAMRKRKYRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T700</accession>
    <name>F0T700_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(NiFe)-hydrogenase-3-type complex Eha, EhaM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644871.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1278"/>
    <dbReference type="EnsemblBacteria" id="ADZ09520">
        <property type="protein sequence ID" value="ADZ09520"/>
        <property type="gene ID" value="Metbo_1278"/>
    </dbReference>
    <dbReference type="GeneID" id="10277729"/>
    <dbReference type="KEGG" id="mel:Metbo_1278"/>
    <dbReference type="eggNOG" id="arCOG04835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133051_0_0_2"/>
    <dbReference type="OMA" id="IDLGLCW"/>
    <dbReference type="OrthoDB" id="101199at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036606">
        <property type="entry name" value="EhaM-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012056">
        <property type="entry name" value="NiFe-hyd_3_EhaM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09218">
        <property type="entry name" value="DUF1959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101332">
        <property type="entry name" value="SSF101332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="16184" checksum="34D5918B6C6A3A62" modified="2011-05-03" version="1">MNSYKPNTEAENEELYITMKKRILASYKWHENVIVPFSKELEISPEELEEILMKRLDMSSLQAINPRYESSKLRCLKERIHADLRLCWLCDIMNILTKEEAKTIRDKIAYNVLENNKNYKEAIEDGRADLLEYLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T706</accession>
    <name>F0T706_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644877.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1284"/>
    <dbReference type="EnsemblBacteria" id="ADZ09526">
        <property type="protein sequence ID" value="ADZ09526"/>
        <property type="gene ID" value="Metbo_1284"/>
    </dbReference>
    <dbReference type="GeneID" id="10277735"/>
    <dbReference type="KEGG" id="mel:Metbo_1284"/>
    <dbReference type="eggNOG" id="arCOG04832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369520_0_0_2"/>
    <dbReference type="OMA" id="NMILLTD"/>
    <dbReference type="OrthoDB" id="83842at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019212">
        <property type="entry name" value="DUF2105_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09878">
        <property type="entry name" value="DUF2105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="23822" checksum="0E0E76C2FBBBE311" modified="2011-05-03" version="1">MILVPDVVSPIVVGLYAPAIIVGILVGLIGLFGISVEKNDLQVLILTDIVGIGMLIFVAAVGTDLAESLILPGLVVELAEIMAISEILMSREMRKSGKTVELIPTPLHFNLEILQTAPIFLAIILIVYGAFLSGFTGGAIAGAGILFYVMSMKVRGVPSGVWEGLAGVSGIAWCLWLVGFLVLFVFPQYWLLGLMLAAFGVFIKVAFKAGLIGVMNREEFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T715</accession>
    <name>F0T715_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644886.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1293"/>
    <dbReference type="EnsemblBacteria" id="ADZ09535">
        <property type="protein sequence ID" value="ADZ09535"/>
        <property type="gene ID" value="Metbo_1293"/>
    </dbReference>
    <dbReference type="GeneID" id="10277744"/>
    <dbReference type="KEGG" id="mel:Metbo_1293"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="VRWMERI"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="312" mass="34452" checksum="7B1CEEAD22E94574" modified="2011-05-03" version="1">MKNKKIVVTGGLGFIGSHLVEKFVDNNEVVIVDNQSTGTIENIKELDISRIDTNFGSINDLNLEEIFEGTDYVFHLAAVTSVPQSVEDPVKSNEVNITGTLKVLEAARKTDVKKLVFSSSSAVYGETEVLPISEEVPINPLSPYAVSKATAELYCNVYSEIYDLPTTCLRYFNVFGPKQDPNSQYAAVIPIFINKLLKNERPTIYGDGEQTRDFVSVERVVEANELAAKSGETGVFNIGLGKSTSINQLFELVKECVKKDMEPVYEPARSGEIKHSVADVSKAKAIGFNPEAEYQQDLIKTIDSIAYNMFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T735</accession>
    <name>F0T735_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646020.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2456"/>
    <dbReference type="EnsemblBacteria" id="ADZ10669">
        <property type="protein sequence ID" value="ADZ10669"/>
        <property type="gene ID" value="Metbo_2456"/>
    </dbReference>
    <dbReference type="GeneID" id="10278928"/>
    <dbReference type="KEGG" id="mel:Metbo_2456"/>
    <dbReference type="eggNOG" id="arCOG01866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_3_2_2"/>
    <dbReference type="OMA" id="GQMSPTY"/>
    <dbReference type="OrthoDB" id="78396at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12844">
        <property type="entry name" value="HTH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="184" mass="21020" checksum="626B132F56B0A7A8" modified="2011-05-03" version="1">MKEKMIEIAQRVKELRELSDIETEDIAASLGVPIETYRNYEEGKLDIPASVLYEIAQILKVDMGLLLTGEETRMHIFTVTRKGKGVGVERRKQYKYENLAEKFIHKKAEPFIVTVKPRKDLGKPSTNSHPGQEFNYVIDGTLKLYIHDNEIVLEKGDSIFFDSSYEHAMEALSGEKAKFLAIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T749</accession>
    <name>F0T749_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646034.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2470"/>
    <dbReference type="EnsemblBacteria" id="ADZ10683">
        <property type="protein sequence ID" value="ADZ10683"/>
        <property type="gene ID" value="Metbo_2470"/>
    </dbReference>
    <dbReference type="GeneID" id="10278943"/>
    <dbReference type="KEGG" id="mel:Metbo_2470"/>
    <dbReference type="eggNOG" id="arCOG01116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098525_0_0_2"/>
    <dbReference type="OrthoDB" id="73767at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="232" mass="25277" checksum="C1227DB2BF47E2D1" modified="2011-05-03" version="1">MIYVGIDDTNNEESVGTAKFTRQVASKVAEKFRVYGVTRHQLAQNSEIDYSIHNFCAVIHVEADKSHLNTIFSIAKTEIMNNFNIGSDPGLATAHEDNISSSVVSFGLDAKYKVVKRDAAIKLARDSEIIIEGFGEAGNGVVGALAGVGLASTKNDGRFVQLGDLRKIKQPEPIQKFLKSGIDKVITVEGVIIEEGIIFNTDNKPVKPSPVNGEIVLFVEPVDNMFRAVNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A7</accession>
    <name>F0T7A7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0083</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643687.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0083"/>
    <dbReference type="EnsemblBacteria" id="ADZ08336">
        <property type="protein sequence ID" value="ADZ08336"/>
        <property type="gene ID" value="Metbo_0083"/>
    </dbReference>
    <dbReference type="GeneID" id="10276506"/>
    <dbReference type="KEGG" id="mel:Metbo_0083"/>
    <dbReference type="eggNOG" id="arCOG00661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033784_1_0_2"/>
    <dbReference type="OMA" id="GYIHLKA"/>
    <dbReference type="OrthoDB" id="20093at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023874">
        <property type="entry name" value="DNA_rSAM_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21180:SF9">
        <property type="entry name" value="PTHR21180:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01102">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="40"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="372" mass="42569" checksum="DD547EB4C1067EB6" modified="2011-05-03" version="1">MNRVRRMEILGESAKYDRCNYGNYNPDNFFSEKLPGVYNATNPNGVCMPLFKVLMTNKCSNDCTYCVNNCANKFNRVELSPSELTSIFLEYYNNKLVEGLFLSSGVPGDIDVSMENMVEVARQLRMDHGYQGYIHLKILPGSSYDMIKRAMSLANRVSINIEAATSEGFNELSSTKNFNTDILRRMAWIKRLMKRDENLAPSGHTTQFIVGASHESDSDILKRVNWLYKKMDIRRSYFSAFNSMEGTPLEGHEEPAPLRTGRLYQADHLVKSYGFDLEELSFEEDGNMILDMDPKYVTAVSNMDMFPIELNTATYKELLRVPGIGKISARRIIESRKRGKQYKSLEELKNIGVNIKKAEIFVKLKNSYQTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B4</accession>
    <name>F0T7B4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643694.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0090"/>
    <dbReference type="EnsemblBacteria" id="ADZ08343">
        <property type="protein sequence ID" value="ADZ08343"/>
        <property type="gene ID" value="Metbo_0090"/>
    </dbReference>
    <dbReference type="GeneID" id="10276513"/>
    <dbReference type="KEGG" id="mel:Metbo_0090"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="43727at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="144"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="5">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="33724" checksum="E847563CBD1FF879" modified="2011-05-03" version="1">MKHLLSVLDAKDEIFEILELAGKFKEDPYGLKPLENKTLAMIFEKASTRTRISFEMAMLHLGGNPLYLSAADMQLGRGEIIEDTAMVMSRYVDGVMIRAKEHEEVVKFSEHSTVPVINGLTNKEHPCQALTDIFTIYEHKQSYNVKMAFLGDGNNVCNSLLLACAYVGMDMTVVCPLGYEPDAEIYDEALKIAEKTGSKIEITPIVSEGVKDADIIYTDVWVSMGDEAEKLKRIEDLKNYQVNMELLTFAKPDACVMHCLPAIRGQEITDEVMKSDSSIIWDQAENRMHVQTAVLYNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B8</accession>
    <name>F0T7B8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="8">KARI</shortName>
            <ecNumber evidence="8">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="8">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvC</name>
        <name evidence="12" type="ordered locus">Metbo_0094</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643698.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0094"/>
    <dbReference type="EnsemblBacteria" id="ADZ08347">
        <property type="protein sequence ID" value="ADZ08347"/>
        <property type="gene ID" value="Metbo_0094"/>
    </dbReference>
    <dbReference type="GeneID" id="10276517"/>
    <dbReference type="KEGG" id="mel:Metbo_0094"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="OrthoDB" id="25435at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
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    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
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        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
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            <begin position="182"/>
            <end position="327"/>
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        <location>
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        <location>
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        <location>
            <position position="194"/>
        </location>
        <ligand>
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            <label>1</label>
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        <location>
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        </location>
        <ligand>
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            <label>2</label>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        </source>
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        </source>
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            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ08347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="36131" checksum="15C293BA02F31DB6" modified="2011-05-03" version="1">MKMYYEKDVDVDILKDKTVAVIGYGSQGMAQSRNMHESGLNVVVGLRKDGASWNKAKEDGLTVMTIEEAAAKADVIHVLIPDEIQAKVYEESIKDNLESGNTISFSHGYNIHFGYINPPKDVNITMIAPKGPGAMVRKTYEEGFGVPGLIAIERDYTGDAFKIALAMGQGSGLTRAGVLETTFKEETETDLFGEQVVLCGGVTELIAAGFQTLVDAGYQPEIAYFETCHEVKLIVDLIYAKGFAGMWNDVSNTAEYGGLSRRKRIITDETRLEMKKALEEIQTGEFAKEWTLENQAGKPMLNRLRAIEASLQIEKVGSKLRKLCGLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B9</accession>
    <name>F0T7B9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0285 protein Metbo_0095</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0285 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0095"/>
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        <property type="protein sequence ID" value="ADZ08348"/>
        <property type="gene ID" value="Metbo_0095"/>
    </dbReference>
    <dbReference type="GeneID" id="10276518"/>
    <dbReference type="KEGG" id="mel:Metbo_0095"/>
    <dbReference type="eggNOG" id="arCOG04885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846254_0_0_2"/>
    <dbReference type="OMA" id="CYSMGDG"/>
    <dbReference type="OrthoDB" id="33713at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01087">
        <property type="entry name" value="UPF0285"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
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    </dbReference>
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        <property type="entry name" value="UCP018783"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="methan_mark_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="35536" checksum="93A2BB240C0C090C" modified="2011-05-03" version="1">MVFVGMDHGTTGVSFTILSDQPVHFKIGRDELSKGKASALKELKNRIDIDSIDLMAITYAMGDGVNKITPINQVKDRGILSMDGAGKVTGGGTAVYDEISESGIPTVLIPGIHKNTECLDPRFKAAYSHHASSEKISICYNAYLETGFENMIVSDISSNSVSMIIEDGKIIGAMDACLGAMGIVHGPLDLEMLRDIDDGIKTANECFSRAGAVKIAEVDTNVSQAKEELILKYIKNEPKAELAFETMLMTVLMEIYGLAGIANNNMEGIVLTGSVGAMETPFNFYGKTKEKLENLAPVVKLPPTSGSVGSAQIAKAVFEGADSILGIDVQKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D4</accession>
    <name>F0T7D4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0110"/>
    <dbReference type="EnsemblBacteria" id="ADZ08363">
        <property type="protein sequence ID" value="ADZ08363"/>
        <property type="gene ID" value="Metbo_0110"/>
    </dbReference>
    <dbReference type="GeneID" id="10276535"/>
    <dbReference type="KEGG" id="mel:Metbo_0110"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="OMA" id="DMLGIIN"/>
    <dbReference type="OrthoDB" id="58376at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02928">
        <property type="entry name" value="TIGR02928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc6_C" evidence="4">
        <location>
            <begin position="300"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="382" mass="44000" checksum="EE7B99BCFB28CA20" modified="2011-05-03" version="1">MNIFEELGEKKSVFKCKKFLDHRFLPEKLPHREEQIKSVAKYWIEALNSVTPPDVTIYGKTGTGKTAVSKFARKQLEQIAKEKKVNVRVEYIRCTDYTTEYQVIARLCQQMGRDVPYRGWTKAEVVHAFRDLFKKNVFGKDLILIIILDEIDILLKNDGDGLLYTLTRTDNVSIASISNFVDFKQFIKPRVRSSLRDREIVFPPYNAQQLVDILKERSEMSFKDGVLNNDVIPLCAALAAKEEGDARYALDLLRTSGELADEKASETVFENYVREAKDYIEHNKVTDIVMTLPSQQQKVLESIIYLTKEKEEITSGRLYEVYKQFSKGDSVSYRRIFDFINELEMLGLISTKTISRGRGKGRTNIIDLQCEMGLLEDAIWNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N0</accession>
    <name>F0T7N0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644949.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1358"/>
    <dbReference type="EnsemblBacteria" id="ADZ09598">
        <property type="protein sequence ID" value="ADZ09598"/>
        <property type="gene ID" value="Metbo_1358"/>
    </dbReference>
    <dbReference type="GeneID" id="10277809"/>
    <dbReference type="KEGG" id="mel:Metbo_1358"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="OrthoDB" id="102399at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="20886" checksum="31E70D07917CDB3C" modified="2011-05-03" version="1">MRAFLAVDLDPNLTYKIMNIQKQLAQVSSNIKLVEEENLHFTFKFLGDITPERKDTIVEMTEEHIKDYAPFNINIKGLGAFPSTDYIKVVWLGVEHPGAFSKVQRELDMEFSKMGFNKERSYIPHLTIGRVKGTKNRDELSALVKELENIEIGSMNIDRLTLKKSELTPVGPIYTDLHDIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P8</accession>
    <name>F0T7P8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644967.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1376"/>
    <dbReference type="EnsemblBacteria" id="ADZ09616">
        <property type="protein sequence ID" value="ADZ09616"/>
        <property type="gene ID" value="Metbo_1376"/>
    </dbReference>
    <dbReference type="GeneID" id="10277827"/>
    <dbReference type="KEGG" id="mel:Metbo_1376"/>
    <dbReference type="eggNOG" id="arCOG02643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_12_2"/>
    <dbReference type="OMA" id="DIYEMAD"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="22879" checksum="8C47A5BC626BD760" modified="2011-05-03" version="1">MPKVVPEYKELAKKKIIKASYTLFESKGYHSTSMDDIAAEVGVSKASLYSYFKSKEDILLVTVDLAITEPFIKTFNENKSVDAFKDYYHTLTVFEGLLHLNYVLTSLANDSDDIKFKLIETYETKLKLLTRFVSKQQSLGELGSDLDPGAVAQFLIAVYGDISLQLIMGVDEKKISNEFEGALKLILENKKIDKDQSTLSSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S8</accession>
    <name>F0T7S8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643766.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0163"/>
    <dbReference type="EnsemblBacteria" id="ADZ08415">
        <property type="protein sequence ID" value="ADZ08415"/>
        <property type="gene ID" value="Metbo_0163"/>
    </dbReference>
    <dbReference type="GeneID" id="10276589"/>
    <dbReference type="KEGG" id="mel:Metbo_0163"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="DGGGRMH"/>
    <dbReference type="OrthoDB" id="72601at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25289" checksum="67AD817516E348FF" modified="2011-05-03" version="1">MYQKMGSELKEILKLEREAVAIKWFLREPKDIKKESEPSRFCTKLDKASKGEIFYSTMEEESCMGGLRYSGMKDRKELPKNMQSGSFLVPAGVYKSIPAVQRSWQNNKAIDAGIFTAILFSPLVNADFIPDIVFIISNSEQAMMVLHANAYDSGSHGLGADSCPICSSMAAIPYLTGKVTYGFGDIGSRNNMELKPDDVMVTIPGAELERILANLKDMQTKTFFKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T9</accession>
    <name>F0T7T9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643777.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0174"/>
    <dbReference type="EnsemblBacteria" id="ADZ08426">
        <property type="protein sequence ID" value="ADZ08426"/>
        <property type="gene ID" value="Metbo_0174"/>
    </dbReference>
    <dbReference type="GeneID" id="10276600"/>
    <dbReference type="KEGG" id="mel:Metbo_0174"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="TQKIGYY"/>
    <dbReference type="OrthoDB" id="22281at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="13"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="463" mass="51544" checksum="3CAA84FEFB455FB6" modified="2011-05-03" version="1">MDIEHYYNKRSYILISLALAPFLSNFVITALNIALPSIALDLNIEAVPLGWIPTSYLITLTIFLIPSAKLGDIHGRKNLFKWGLILFTAGSIITVFSWSYHCIIFSRILQGMGSAMIFGNLYAMIASIYIHQDRIRALSIIFCSIFLGSFLGPIIGGLLSGFYNWRVIFVFCSLIGMVGILLLSRLDVEWFGLKGEKFDLVGSLMFGISSLLIIQGLSKINQELNWIFLVLGIIELTVFILYENQVDYPLFNLEVLHNRDFILNSAGTFIQYAPIAPIVFILNIYLQNVRGFSPILVGFILVLQPLAMLVVSIFSEKLNRLIKAPTMVTIAMIISLMSVLVFLTLKLSTPILVIILGLTLSGIGTSLFSSPNSHLIMNSVEKKYYGVASASITTMRGFGGSLGMSIVLVILTLVVGNIEINQLHSESFIISLNLIFRILCILYIAGTVIMLLNWYDVKNKNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U5</accession>
    <name>F0T7U5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name evidence="4" type="ordered locus">Metbo_0180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643783.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0180"/>
    <dbReference type="EnsemblBacteria" id="ADZ08432">
        <property type="protein sequence ID" value="ADZ08432"/>
        <property type="gene ID" value="Metbo_0180"/>
    </dbReference>
    <dbReference type="GeneID" id="10276606"/>
    <dbReference type="KEGG" id="mel:Metbo_0180"/>
    <dbReference type="eggNOG" id="arCOG00638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_2_0_2"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="OrthoDB" id="104636at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="44"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="167"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="341" mass="36916" checksum="7D0D47AED1867298" modified="2011-05-03" version="1">MPSDKPKITELGEKKLIKRLLSRSQTPRVKNLFLNKIPIESLSDDAALIDIGENYLVLCSDMLMRSSHFPVEMSFRQIGQKVVAVNVSDLAAMGADAVGIIISMGLPKTMLVEEFDDLVEGILKACDKYGMALIGGDTNEAAELTLNGTCIGTVKKENVLMRSGALPGDVIAVTGDLGLAAAGFEILGCDCQDELNEESVKICLKHALEPEARLKEGIILSKNGNVSSATDISDGLLSELGEIIDANESTIGITVHENWFPIPSEVFEIAEITHKNPYDLALSYGEDFELLLTISPSEFESIKTKLKLKKIGFVDSTGTIKMIDKSGNTKVVTPKGYEHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X9</accession>
    <name>F0T7X9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643817.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0214"/>
    <dbReference type="EnsemblBacteria" id="ADZ08466">
        <property type="protein sequence ID" value="ADZ08466"/>
        <property type="gene ID" value="Metbo_0214"/>
    </dbReference>
    <dbReference type="GeneID" id="10276640"/>
    <dbReference type="KEGG" id="mel:Metbo_0214"/>
    <dbReference type="eggNOG" id="arCOG03589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069348_0_0_2"/>
    <dbReference type="OMA" id="AYMLDRW"/>
    <dbReference type="OrthoDB" id="109304at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016874">
        <property type="entry name" value="TcmP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028177">
        <property type="entry name" value="Polyketide_synth_Omtfrase_TcmP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="271" mass="31864" checksum="32F170D72874A01F" modified="2011-05-03" version="1">MKLKLKGVQETLLIPLWSKAMEVEQPNPIVTDHKAVEIVKNLDYNFPNLDKEWATQLSVVIRTELLDKAVEKFMAEHKNGAVINLGCGLDTRYSRLDNKNYSWFDLDLPSTIELRRNFFDETNSYHMIDKSVFDYGWFNQIPGDVPLLIIAEGLLMYFSEEEVSELFKALAEKFSGAEMLIETIPYSLVKQSQNSNLIEKQYNIKANFSWGINNGNEVEKLNPNIKYIEDWHYFDYHRDRWKIIRWLSLIPYFKNRFGNRIVHFKFSMGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y9</accession>
    <name>F0T7Y9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643827.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0224"/>
    <dbReference type="EnsemblBacteria" id="ADZ08476">
        <property type="protein sequence ID" value="ADZ08476"/>
        <property type="gene ID" value="Metbo_0224"/>
    </dbReference>
    <dbReference type="GeneID" id="10276652"/>
    <dbReference type="KEGG" id="mel:Metbo_0224"/>
    <dbReference type="eggNOG" id="arCOG06492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154336_0_0_2"/>
    <dbReference type="OMA" id="IMHEVGY"/>
    <dbReference type="OrthoDB" id="114202at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="15980" checksum="3C1AAB9FF827D071" modified="2011-05-03" version="1">MVAIDQNVFYIIIAAIVVVGLIVALMQWRRVREAQTNVDFLTKQAELKKIELVEKDLESKRMMNDIVLPKDQQEKLTQIRENTSDLMHKAGYLHSEINERVNRLEAQTEFAKLQKLLMDIEKKEQELEKKSGKGKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T870</accession>
    <name>F0T870_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">grpE</name>
        <name evidence="7" type="ordered locus">Metbo_0230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643833.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0230"/>
    <dbReference type="EnsemblBacteria" id="ADZ08482">
        <property type="protein sequence ID" value="ADZ08482"/>
        <property type="gene ID" value="Metbo_0230"/>
    </dbReference>
    <dbReference type="GeneID" id="10276660"/>
    <dbReference type="KEGG" id="mel:Metbo_0230"/>
    <dbReference type="eggNOG" id="arCOG04772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_5_2_2"/>
    <dbReference type="OMA" id="YAYEKIA"/>
    <dbReference type="OrthoDB" id="114121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0346">Stress response</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="20876" checksum="052942F260EA0843" modified="2011-05-03" version="1">MTDKQELEKLKKDLDSLKTEIKEKDEIIQNKDQEIQDLGDKAEEYHSQLLRLHADFENYKKRSEKDLKEFIKYANEELIVKIIDVYEDLERALKADDSQDIKEGVVMIHKKLKDTLKNEGLCEIETSGEPFDPYKHEALMVEDNEDYEDGTIIEELAKGYSLDSKVIKYSKVKVCKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A2</accession>
    <name>F0T8A2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Energy-converting hydrogenase B, subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643865.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0262"/>
    <dbReference type="EnsemblBacteria" id="ADZ08514">
        <property type="protein sequence ID" value="ADZ08514"/>
        <property type="gene ID" value="Metbo_0262"/>
    </dbReference>
    <dbReference type="GeneID" id="10276694"/>
    <dbReference type="KEGG" id="mel:Metbo_0262"/>
    <dbReference type="eggNOG" id="arCOG03076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173139_2_1_2"/>
    <dbReference type="OMA" id="MAYLYYA"/>
    <dbReference type="OrthoDB" id="125479at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="8">
        <location>
            <begin position="9"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8410" checksum="B3235927570119D3" modified="2011-05-03" version="1">MIEYVFMITTILGAIITLLQRDLLKAAILTGIPGASLALLYQYLQAPDVALTQAIVGSAIVPVFFALAVYRTRRVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J2</accession>
    <name>F0T8J2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645094.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09743">
        <property type="protein sequence ID" value="ADZ09743"/>
        <property type="gene ID" value="Metbo_1512"/>
    </dbReference>
    <dbReference type="GeneID" id="10277963"/>
    <dbReference type="KEGG" id="mel:Metbo_1512"/>
    <dbReference type="eggNOG" id="arCOG11555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177701_0_0_2"/>
    <dbReference type="OMA" id="DEHTHIT"/>
    <dbReference type="OrthoDB" id="139902at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024997">
        <property type="entry name" value="DUF3892"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13031">
        <property type="entry name" value="DUF3892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="99" mass="11376" checksum="18C952DDD3494813" modified="2011-05-03" version="1">MEIDRINYWISEVCIKDGHIHSVRLYRDSELAYGLDQTWTVQQVIDTINKGYNINTIVNVKGRWVSGARVKIMTIGGTSYITTERDESAPDNLENLPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M0</accession>
    <name>F0T8M0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0310"/>
    <dbReference type="EnsemblBacteria" id="ADZ08562">
        <property type="protein sequence ID" value="ADZ08562"/>
        <property type="gene ID" value="Metbo_0310"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0310"/>
    <dbReference type="eggNOG" id="arCOG12638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623466_0_0_2"/>
    <dbReference type="OMA" id="CKLRNDG"/>
    <dbReference type="OrthoDB" id="111938at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="17419" checksum="A6E5A1A180AA2689" modified="2011-05-03" version="1" precursor="true">MMDNKIIALIVVVIVLLVAGVVAFSLTGNNNMTNNNSTIANNSQNTVSNSSGAVKVVAKQSGPETAKKGDNVTINYTVSNEGSAVVQNVKISSQNFENTIGTLNVNETKSYSYTLHIPTDEEVQADFGPNSTVSNPFSIGGFSVSFTDSSGSTHSINANSIEIKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R4</accession>
    <name>F0T8R4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 12">Tetrahydromethanopterin S-methyltransferase subunit E</fullName>
            <ecNumber evidence="7 12">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mtrE</name>
        <name evidence="13" type="ordered locus">Metbo_0354</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the MtrE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="7 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643957.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0354"/>
    <dbReference type="EnsemblBacteria" id="ADZ08606">
        <property type="protein sequence ID" value="ADZ08606"/>
        <property type="gene ID" value="Metbo_0354"/>
    </dbReference>
    <dbReference type="GeneID" id="10276791"/>
    <dbReference type="KEGG" id="mel:Metbo_0354"/>
    <dbReference type="eggNOG" id="arCOG04870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958513_0_0_2"/>
    <dbReference type="OMA" id="QMGNIHR"/>
    <dbReference type="OrthoDB" id="50292at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01098">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005780">
        <property type="entry name" value="MeTrfase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04206">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016509">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01113">
        <property type="entry name" value="mtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="12 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="30808" checksum="04F0C27B0D4CC50C" modified="2011-05-03" version="1" precursor="true">MDPMITGLGIVALMGAAATIAGAAEDLESDVGSMSNPNSQVQLAPQMGHLHRMFNKAISGEPVQMGTLAGIAGSVAFVLMSSVHLPVIMSIAAGGFIAALFHSAFATTSYLGRIVGQSQFDQPVFMDVITGHLGPIAAHGFIVTFCIVGLSYLMTLPVQGFNHPFPLPLLAVLWGITIGAIGSSTGDVHYGAEREYQTYPFGGGIPVAIHGDITTMSELGPRNSIDVVHFCAKYGGPVTGFCFGLIVFFSFWTTVVFGPTGGVIAGLVIVLAIIILNNRIELFARNQYGPYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T9</accession>
    <name>F0T8T9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645118.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1537"/>
    <dbReference type="EnsemblBacteria" id="ADZ09767">
        <property type="protein sequence ID" value="ADZ09767"/>
        <property type="gene ID" value="Metbo_1537"/>
    </dbReference>
    <dbReference type="GeneID" id="10277992"/>
    <dbReference type="KEGG" id="mel:Metbo_1537"/>
    <dbReference type="eggNOG" id="arCOG01787">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243991_0_0_2"/>
    <dbReference type="OMA" id="TFENIRP"/>
    <dbReference type="OrthoDB" id="60517at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_12" evidence="1">
        <location>
            <begin position="48"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="26636" checksum="91EA58ACA7730667" modified="2011-05-03" version="1">MPAKDNSSSHRSEDYDLQIGDTIPYYESFHDETLNMVKTILKEPKIWLDTGCGTGTLVNKAVQEFDTTSFILSDPSPEMLNLAREKLSNQTKRLRILNPNETSNISLGNDLKPDVITAIQAHHYLSKEERTETTKVCYDLLRDDGVYITFENIRPKTEEGIRVVKEYVKNYQLKRGRDEETVNNFLKRFDVEFFPIKVEEHISLLEKTGFKVVELFWMSYMQAGFYCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y1</accession>
    <name>F0T8Y1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645160.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1582"/>
    <dbReference type="EnsemblBacteria" id="ADZ09809">
        <property type="protein sequence ID" value="ADZ09809"/>
        <property type="gene ID" value="Metbo_1582"/>
    </dbReference>
    <dbReference type="GeneID" id="10278038"/>
    <dbReference type="KEGG" id="mel:Metbo_1582"/>
    <dbReference type="HOGENOM" id="CLU_1773203_0_0_2"/>
    <dbReference type="OrthoDB" id="100211at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16670" checksum="ACD6E47C296964FA" modified="2011-05-03" version="1" precursor="true">MKTKLLPLLLMVMFAFSVAQPIFASNPQLEDTKIADKFGNPLKDITVNAGETIELQARLYWFYYTVDDSKPVWIPQVVRYLDFYVYKLNANGTNGDLVWSDTAVTNFFTANANPDTFKLEKGNYRLFVKYDGSLKPCNTTAKIYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T945</accession>
    <name>F0T945_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lactaldehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-lactaldehyde + H2O + NAD(+) = (S)-lactate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644018.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0415"/>
    <dbReference type="EnsemblBacteria" id="ADZ08667">
        <property type="protein sequence ID" value="ADZ08667"/>
        <property type="gene ID" value="Metbo_0415"/>
    </dbReference>
    <dbReference type="GeneID" id="10276852"/>
    <dbReference type="KEGG" id="mel:Metbo_0415"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OMA" id="WHKLIEQ"/>
    <dbReference type="OrthoDB" id="42527at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="8">
        <location>
            <begin position="12"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="468" mass="50450" checksum="5420041A3FFF14F2" modified="2011-05-03" version="1">MKMIINGSLVDSDEKIDVVNPVNNQVIDTVPSGTIEDVKTALETAYEAQKVFANYSSRKVSRIMYDIYEDLKQNSKELADLLTRETGKPIKDSNDEIKRSIETVLLAAEESKRIYGETVPLDAGIGGRTALGFTVKVPLGIVTAITPFNYPVNLAVHKIAPAIAAKNAVILKPSTQAPLAALKMVQIFNSHLPAGVVSAVTGKSSVIGDELVTNPRVNKISFTGSVETGVSISEKAGMKRINMELGGNDPLIVLEDADIDRAVEAAIKGSFLFSGQVCIAVKRIILEESIADEFAAKMVQRTKKLKVGDPLNPETDMGPMIDEQAAINVENLVNNAKSSGAKLLVGGEREGAFYLPTILDHVETSMDMVCNETFGPVAPLLRVKNLDEAINIANDTQYGLQAGIFTKSIENALKAAREIEAGSVIINRQSTFRTDNMPFGGFKMSGTGKEGIKYAVEDMTRSKLIIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T949</accession>
    <name>F0T949_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadX</name>
        <name evidence="9" type="ordered locus">Metbo_0419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644022.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0419"/>
    <dbReference type="EnsemblBacteria" id="ADZ08671">
        <property type="protein sequence ID" value="ADZ08671"/>
        <property type="gene ID" value="Metbo_0419"/>
    </dbReference>
    <dbReference type="GeneID" id="10276856"/>
    <dbReference type="KEGG" id="mel:Metbo_0419"/>
    <dbReference type="eggNOG" id="arCOG00254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089550_0_0_2"/>
    <dbReference type="OMA" id="ECAGHSA"/>
    <dbReference type="OrthoDB" id="50102at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="DUF108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="8">
        <location>
            <begin position="7"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF108" evidence="7">
        <location>
            <begin position="155"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="27004" checksum="21183995F5E53C45" modified="2011-05-03" version="1">MKVGILGCGAIANIITNFAVEGKLGVDLNFFYDRDMERAENLASQVDGTVALDINSMLENVDLVIEAAAPQAVSETVPLVLEHGVDVIIMSIGALMDTELRKTLENLAAKNNAKIYAPSGAIVGLDGIKAASIGKIQSATLVTRKPPRSLGVSAEEETILYEGKAGDAVRKFPMNINVAAALSLACGKEVDVKIIADPSVDRNMHEVQVIGDFGEIKTITQNVRCSMNPKTSVMAAYSAIKLLNSLNETFRIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A6</accession>
    <name>F0T9A6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADZ09857"/>
        <property type="gene ID" value="Metbo_1631"/>
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    <dbReference type="GeneID" id="10278088"/>
    <dbReference type="KEGG" id="mel:Metbo_1631"/>
    <dbReference type="eggNOG" id="arCOG02994">
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11517" checksum="D4B81EA74612812F" modified="2011-05-03" version="1">MSSIPDVMDVAKSGYNLLLENNRVRVLEIRLKPGEKSPMHNHPNDHVIYVVKDAEFKLYFPNGNTEVFKLVEGQVKWIKAGSHQTENVGSTEGHCIVVETKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F8</accession>
    <name>F0T9F8_METLA</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644057.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0454"/>
    <dbReference type="EnsemblBacteria" id="ADZ08706">
        <property type="protein sequence ID" value="ADZ08706"/>
        <property type="gene ID" value="Metbo_0454"/>
    </dbReference>
    <dbReference type="GeneID" id="10276892"/>
    <dbReference type="KEGG" id="mel:Metbo_0454"/>
    <dbReference type="eggNOG" id="arCOG00517">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_7_1_2"/>
    <dbReference type="OMA" id="QVWPGHG"/>
    <dbReference type="OrthoDB" id="8880at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallo-B-lactamas"/>
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        <property type="entry name" value="POD-like_MBL-fold"/>
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        <property type="entry name" value="Rhodanese-like_dom_sf"/>
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        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="262"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="366"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="455" mass="51297" checksum="FC285A7B9DF48DF9" modified="2011-05-03" version="1">MILETVKSEGIAHKSYFIGSNGIAAVVDPRRDCDIYIDIAEKNNMRINYIFETHRNEDYTAGSLELREVVGADIFHGKGLDFAYGNSVVEGDKFQIGNVVLEIIETPGHTNESISLLLKDREVSEYPYMVFTGDTIFAGEVGRCDLYGNNETLKMAKAMYNSVFNKLLKLDDNVIILPAHGSGSVCGAVIREQEFTTVGYEKKTNPALKKSEKEFLEFKLHEKLYTPPYFKEMEKNNLIGPSLICKLPYLKPLGIHELKELMSKGGQVVDVRDPAAFAGGHIPNTLNIWKDGLPSYSGWFLNYEQPIIVVDENNSNIEDLKRYLIRLGYDNVYGYLSGGFPVWFKGSGDFETVHTWSVHDLLPNMGKKSLFILDVRKENDWEKERIEGSHNIYIGELKENLDKVPRNKHVAVLCDTGYKASIGTSILKMNGYKNVTNVMGSMTAWKKAGYPVVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G4</accession>
    <name>F0T9G4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0460"/>
    <dbReference type="EnsemblBacteria" id="ADZ08712">
        <property type="protein sequence ID" value="ADZ08712"/>
        <property type="gene ID" value="Metbo_0460"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0460"/>
    <dbReference type="eggNOG" id="arCOG01819">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="OMA" id="YHDNWLS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="168"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="541" mass="61008" checksum="D844553D1DB587ED" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S4</accession>
    <name>F0T9S4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645304.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1729"/>
    <dbReference type="EnsemblBacteria" id="ADZ09953">
        <property type="protein sequence ID" value="ADZ09953"/>
        <property type="gene ID" value="Metbo_1729"/>
    </dbReference>
    <dbReference type="GeneID" id="10278186"/>
    <dbReference type="KEGG" id="mel:Metbo_1729"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="OMA" id="DRPMNAN"/>
    <dbReference type="OrthoDB" id="106121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="11706" checksum="89D05BD3D5AC8393" modified="2011-05-03" version="1">MKHLEKLLWWLLTGKRGGTNRARIIEKLRERPYNPHQLSKELNLDYKTIRHHIKILRDNNIIKATGDSYSKLYFLTDAMEENYEVFQEIWDNYIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T6</accession>
    <name>F0T9T6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645316.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1742"/>
    <dbReference type="EnsemblBacteria" id="ADZ09965">
        <property type="protein sequence ID" value="ADZ09965"/>
        <property type="gene ID" value="Metbo_1742"/>
    </dbReference>
    <dbReference type="GeneID" id="10278199"/>
    <dbReference type="KEGG" id="mel:Metbo_1742"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_11_2"/>
    <dbReference type="OMA" id="TGYANEP"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="16736" checksum="47FCFCB93D8D1FF5" modified="2011-05-03" version="1">MLDANILIVEDEFIEAADLKKRLERLGYSVSGIVGTGEAAIQKAEELFPDLILMDIMLKGSMNGIQAADQIMEKSDIPVIYLTAYYDNDTLEKAKKTAPYGYIIKPYEDMGLRSAIEMAVYNHHNEQRLKNSAKVLKFSSEMLKEINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T7</accession>
    <name>F0T9T7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645317.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1743"/>
    <dbReference type="EnsemblBacteria" id="ADZ09966">
        <property type="protein sequence ID" value="ADZ09966"/>
        <property type="gene ID" value="Metbo_1743"/>
    </dbReference>
    <dbReference type="GeneID" id="10278200"/>
    <dbReference type="KEGG" id="mel:Metbo_1743"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_43_3_2"/>
    <dbReference type="OMA" id="RPGFGTC"/>
    <dbReference type="OrthoDB" id="69240at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="27"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="284" mass="31719" checksum="EC386FFC6DDF58F2" modified="2011-05-03" version="1">MRYEKEFVSSSDSTVVGYRKMGNGPGLVLLHGGANSSQHFMELGECLSEDFTVYIPDRRGRGLSGDFGDQYCMEREMEDLDVVLRKTGAKYIFGLSSGALIALEAALNLPITKAAIYEPPLDIDNNVLSIMSFMKRFDREMAEGNIAAGTATMTKDFGSHFGLPKTITKLPHFLLELIFKVYLKTERVKEDEVPFNVLIPTFHYDYELVKEESGQVEKFRNLKAEVLLIGGTESPTFLKKTMKKLKNTIPNVRQVELSGLSHTGPLVTGNPKLVAEKLKDFFCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U6</accession>
    <name>F0T9U6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitrite reductase (NAD(P)H)</fullName>
            <ecNumber evidence="5">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.1.4" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645326.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1752"/>
    <dbReference type="EnsemblBacteria" id="ADZ09975">
        <property type="protein sequence ID" value="ADZ09975"/>
        <property type="gene ID" value="Metbo_1752"/>
    </dbReference>
    <dbReference type="GeneID" id="10278209"/>
    <dbReference type="KEGG" id="mel:Metbo_1752"/>
    <dbReference type="eggNOG" id="arCOG02056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072599_0_0_2"/>
    <dbReference type="OMA" id="RNACGSV"/>
    <dbReference type="OrthoDB" id="58856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017220">
        <property type="entry name" value="Sulphite_reductase_assimil"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037487">
        <property type="entry name" value="Sulfite_red_assimil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIR_SIR_ferr" evidence="4">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="3">
        <location>
            <begin position="86"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="24965" checksum="061E908012C3A476" modified="2011-05-03" version="1">MVTKENIPEKGAAVQKDMKSFAIIPYIPGGLVDPKTLHKIADVADKYGVKIIKLNSEHRIGFYGVKEEDIDPIWDDLDMEPGGFTGKCVRASKFCIGNSSCKKAYQNTINIGLKIDEKFHRMKTPNKVKIAISGCTNSCGESNVRDIGLIGTPKGWKLLVGGTCGLQARPGRLLAENLSESQVMIYIEKILDYYVEKNLPNRMGVFIEKIGFERFKKDINTSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X9</accession>
    <name>F0T9X9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644153.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0550"/>
    <dbReference type="EnsemblBacteria" id="ADZ08802">
        <property type="protein sequence ID" value="ADZ08802"/>
        <property type="gene ID" value="Metbo_0550"/>
    </dbReference>
    <dbReference type="GeneID" id="10276995"/>
    <dbReference type="KEGG" id="mel:Metbo_0550"/>
    <dbReference type="eggNOG" id="arCOG01788">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081790_1_0_2"/>
    <dbReference type="OMA" id="GVFYNAD"/>
    <dbReference type="OrthoDB" id="49237at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005271">
        <property type="entry name" value="CmoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF2">
        <property type="entry name" value="PTHR43861:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="47"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="26616" checksum="2F4B7C498B2D2BAB" modified="2011-05-03" version="1">MEHVKEHFEEEAKEFDDTIVKLIPMYSQMIDSMILTIPQEVSDEFKVLDLGTGTGNVSKAIKEKFKHASIDCIDIAEKMIEMAKIKLEGYTDIHYYTGDFYEFEFEEKYDVVVSSLALHHIKTDDEKKKFYHKIYHILKEGGIFINSDSVLGSNERMQKLNKEKWKEFMLNNVSEDEVEDKWVPQSKKEDYPAPITNHLKWLEEAGFKDLDIVWKYYGWAVYCGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y0</accession>
    <name>F0T9Y0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dUTP diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.23</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.23" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644154.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0551"/>
    <dbReference type="EnsemblBacteria" id="ADZ08803">
        <property type="protein sequence ID" value="ADZ08803"/>
        <property type="gene ID" value="Metbo_0551"/>
    </dbReference>
    <dbReference type="GeneID" id="10276996"/>
    <dbReference type="KEGG" id="mel:Metbo_0551"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103451_2_0_2"/>
    <dbReference type="OMA" id="EYNGSYQ"/>
    <dbReference type="OrthoDB" id="94658at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="2">
        <location>
            <begin position="57"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="17140" checksum="6D4BD839713F9158" modified="2011-05-03" version="1">MIGENELKKLFPDFEELVQPSGIDLRIDEVFIQRGPGSLIDNEKELPEIEKLEPPIYVLKPKTSYLVTVDRKIKIPKGYSMLYVPRSTLLRSFVSVHTAVGDPGFYGTLQFMVYNYGDFDYKVKKGERIAQGVVFDVSGSGEYNGSYQESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y1</accession>
    <name>F0T9Y1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644155.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08804">
        <property type="protein sequence ID" value="ADZ08804"/>
        <property type="gene ID" value="Metbo_0552"/>
    </dbReference>
    <dbReference type="GeneID" id="10276997"/>
    <dbReference type="KEGG" id="mel:Metbo_0552"/>
    <dbReference type="eggNOG" id="arCOG04253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061722_0_0_2"/>
    <dbReference type="OMA" id="VIQQGMN"/>
    <dbReference type="OrthoDB" id="61040at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008482">
        <property type="entry name" value="DUF763"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597">
        <property type="entry name" value="PTHR38597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05559">
        <property type="entry name" value="DUF763"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="374" mass="42609" checksum="43B04190BAB04BB8" modified="2011-05-03" version="1">MMQRTVANLPLHGGKAPRWLFHRMVKLTEGIVDVMLYEYDANEFLRRISNPYWFQAFSCVLGFDWHSSGTTTTTCGALKSAINPEKHGIVVGGGKGKASRKTPSDIENAGEIFSLSTNKIEDLIHSSKISAKIDNSCVQDGYQLYHHSFILTEKGKWAVIQQGMNQEKRYARRYHWLGEEVDELTVDPHRGICCDDETAKTLNMTADKSDATRDISVDLICDNPEHLKKYFKDRTIIPKSQSKLDCYFNEYEMPKHHPVLDEDLSDREFEVLKQACEIQPSNYEELISLNGIGPKKIRALALISDLVYGSKPSWEDPVKYSFTHGGKDGFPYPVDREVYDHSIITIKEALEDAKINKKDKYNALKRLETHIKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA04</accession>
    <name>F0TA04_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase class-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644178.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0576"/>
    <dbReference type="EnsemblBacteria" id="ADZ08827">
        <property type="protein sequence ID" value="ADZ08827"/>
        <property type="gene ID" value="Metbo_0576"/>
    </dbReference>
    <dbReference type="GeneID" id="10277021"/>
    <dbReference type="KEGG" id="mel:Metbo_0576"/>
    <dbReference type="eggNOG" id="arCOG00095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077077_0_0_2"/>
    <dbReference type="OMA" id="FKGVGFP"/>
    <dbReference type="OrthoDB" id="55229at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="305" mass="34004" checksum="0B01307A56A05640" modified="2011-05-03" version="1">MCGIAGVVYKDKKLHHVGDDLTKMLDALQHRGPDSAGLAIYGGLGLKENEYLLNIEVKEKHGLLEAVKETVNTATPIKKEEIIPSVENYMIYRCKIGLSSFSELKPLIMDIDKIDNVTVLNGAHSFEMIKDVGLVKDIAARYDTTSKMGTHGIGHTRFSTESLVDRYHAHPFQSYIIPDITVVHNGQITNYWKIRDPLERKGHIFETNNDTECIVHYIADKLSEGYKLEEALEQSVKDMDGPFSYIVGTPNGVGIAKDQLGLRPGVMAENNEVFAIASEEVSLREVMDTHDIDQIAPGETRSYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA15</accession>
    <name>F0TA15_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sua5/YciO/YrdC/YwlC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644189.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0587"/>
    <dbReference type="EnsemblBacteria" id="ADZ08838">
        <property type="protein sequence ID" value="ADZ08838"/>
        <property type="gene ID" value="Metbo_0587"/>
    </dbReference>
    <dbReference type="GeneID" id="10277032"/>
    <dbReference type="KEGG" id="mel:Metbo_0587"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_2_2"/>
    <dbReference type="OMA" id="MLYPTDT"/>
    <dbReference type="OrthoDB" id="52427at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="22367" checksum="B2FB0A85C891D35E" modified="2011-05-03" version="1">MKILKINNKNPDPDTITQAKEYLKQGKIVVYPTDTVYGIAANAYNESAVSKVFDTKKRLKTKAVSLCLAEIEAINDVAFMDNKTEILVEQLFPGPFTVIMRKKEQISPLLTGGTNKIGIRIPDNKLSRDLASEFPITSTSANISGLNVPETPKEVVEQLGDSVDLVLDAGVCKYGYHSTVVDMTGDKPIILRMGAGYETLMNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA28</accession>
    <name>F0TA28_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="3">2.7.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="3">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3'-dephospho-CoA + GTP = CoA + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644202.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0600"/>
    <dbReference type="EnsemblBacteria" id="ADZ08851">
        <property type="protein sequence ID" value="ADZ08851"/>
        <property type="gene ID" value="Metbo_0600"/>
    </dbReference>
    <dbReference type="GeneID" id="10277045"/>
    <dbReference type="KEGG" id="mel:Metbo_0600"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="OMA" id="AIYDHKT"/>
    <dbReference type="OrthoDB" id="116732at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="PTHR40732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="18697" checksum="84A4788241E9261B" modified="2011-05-03" version="1">MLILKQDMRSEFKKPIGTLFPTISDAKDSLDKKGEKGLLISIGDVTTRKMLDEGIIPDVGIVDNLIEREPSDYEICYDNVTLKTKNPPGTITDQMWKTIKEGFSLVEKAGYNVLIVVDGEEDLSAIPCMVMAPEGSIIFYGQPGEGVVLCEVDKTKEKAQDLIKKLEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA51</accession>
    <name>F0TA51_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RHH_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645352.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10001">
        <property type="protein sequence ID" value="ADZ10001"/>
        <property type="gene ID" value="Metbo_1779"/>
    </dbReference>
    <dbReference type="GeneID" id="10278236"/>
    <dbReference type="KEGG" id="mel:Metbo_1779"/>
    <dbReference type="HOGENOM" id="CLU_3094106_0_0_2"/>
    <dbReference type="OrthoDB" id="140899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="51" mass="5827" checksum="2FACBA1F7E40DBF4" modified="2011-05-03" version="1">MSKKIAVGLRLDEVQLEQLKTFSKRKGGSPISHHIRIAIAEYLEKYDNSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA83</accession>
    <name>F0TA83_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645384.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1811"/>
    <dbReference type="EnsemblBacteria" id="ADZ10033">
        <property type="protein sequence ID" value="ADZ10033"/>
        <property type="gene ID" value="Metbo_1811"/>
    </dbReference>
    <dbReference type="GeneID" id="10278268"/>
    <dbReference type="KEGG" id="mel:Metbo_1811"/>
    <dbReference type="eggNOG" id="arCOG03432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026556_1_0_2"/>
    <dbReference type="OMA" id="EHPPESI"/>
    <dbReference type="OrthoDB" id="54190at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018702">
        <property type="entry name" value="DUF2207_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09972">
        <property type="entry name" value="DUF2207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="613" mass="68584" checksum="FC6D9FFE8CE9429D" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA5</accession>
    <name>F0TAA5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S6e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ordered locus">Metbo_0606</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644208.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0606"/>
    <dbReference type="EnsemblBacteria" id="ADZ08857">
        <property type="protein sequence ID" value="ADZ08857"/>
        <property type="gene ID" value="Metbo_0606"/>
    </dbReference>
    <dbReference type="GeneID" id="10277051"/>
    <dbReference type="KEGG" id="mel:Metbo_0606"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="OMA" id="DGFPMRP"/>
    <dbReference type="OrthoDB" id="112720at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_S6e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_S6e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_S6e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="PTHR11502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13710" checksum="D3A500EE01739AED" modified="2011-05-03" version="1">MAFKLVISEGEKSHQMEVEAAESKKLNGLTIGEEFDATLVGLTGYKLKITGGSDKNGFPMKKDVDGSRRIKSLLAGGIGFNPKRDGQRRRKTVRGNTVSDDIVQINTVVTQKGEKEINEILESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC6</accession>
    <name>F0TAC6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0627</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-methylglutaryl-CoA + 2 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644229.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0627"/>
    <dbReference type="EnsemblBacteria" id="ADZ08878">
        <property type="protein sequence ID" value="ADZ08878"/>
        <property type="gene ID" value="Metbo_0627"/>
    </dbReference>
    <dbReference type="GeneID" id="10277072"/>
    <dbReference type="KEGG" id="mel:Metbo_0627"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OMA" id="DDKMTRA"/>
    <dbReference type="OrthoDB" id="26445at2157"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="PTHR10572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="SSF55035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="SSF56542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="42716" checksum="329F66DC585BDE95" modified="2011-05-03" version="1">MADEKELINKIADGQVKLHQVEKFTDDVDEAVTIRRKFVEKVSNTELKHISKYSLSMEEALKRNIENPIGTVQIPVGIAGPLAIHGEHAEGEYYVPIATSEGALIASINRGFSAIRAGGGATARIIDDKMTRAPVIKTSSVSESLKIKTWIQTNFSKLKEAAESTTRHGKLVKIDPVVVVGKYVYPRFVYTTGDSMGMNMVTIATETALNVLMEETSGHVISLSGNVDVDKKPAAMNLIEGRGKTISAEISLPREVVAKTLKTTPEAIVEVNTAKNFVGSAISGSMGFNAHYANMIGGIFLATGQDEAHIVEGSLGITVAEENDGNLYFSVTLPDVPVATFGGGTRLETARECLEIMGVYGSGGVGKFAEIVAGTVLAGELSLMGALSAGHLARAHKDLGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD8</accession>
    <name>F0TAD8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogen dehydrogenase</fullName>
            <ecNumber evidence="3">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644241.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0639"/>
    <dbReference type="EnsemblBacteria" id="ADZ08890">
        <property type="protein sequence ID" value="ADZ08890"/>
        <property type="gene ID" value="Metbo_0639"/>
    </dbReference>
    <dbReference type="GeneID" id="10277084"/>
    <dbReference type="KEGG" id="mel:Metbo_0639"/>
    <dbReference type="eggNOG" id="arCOG01549">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="QNNLAMN"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="482" mass="52717" checksum="D0A7B766620BB357" modified="2011-05-03" version="1">MKQIEISPVSRIEGHAKITVQLDDAGNVSDAHFHVMEIRGFEKFLEGAAVEEAPRITPRICGVCQTAHHVVSAKATDAVFGLEPPETGKKLRELSLLGQYIHSHALHFYFLGGPDLVLGPDSDPAERNVVGIIKKNPDLAKMAIKTRKIGQEITTVVGGKPIHPVTSIPGGQSKVLTSGERDALLPKVQEGIGLVETGLEVAKPLFETYANEIESLGPVETYFGSLTNGGALELYDGPMKIIDKNGGAVTEFDTVNYLDYIEEKVQPWSYMKFPYLKQIGFPEGNYRVGPLARLNIADSVNTERASALFGEFKDNFGTAQNPLLYHYARLVELMYCVERSIQLLEDDSITSTDIRTDLSEPLMSTADAKNSDEKMRGVGVIEAPRGTLFHDYETDGAGIIQKANLIVASGQNNLSMDIGVRETAKAMIKGDEVSEGLKNRLEMIVRAYDPCLSCATHMINGESSMLLDIHDSDGSLIKRQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF1</accession>
    <name>F0TAF1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0652"/>
    <dbReference type="EnsemblBacteria" id="ADZ08903">
        <property type="protein sequence ID" value="ADZ08903"/>
        <property type="gene ID" value="Metbo_0652"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0652"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="OMA" id="NVGRIRY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="12809" checksum="B8757DD815C82E01" modified="2011-05-03" version="1">MVEMIERILKDLGRINGVSGSLVVGKDGLIIESEVPGDIDSELVAAMSSAVFGTAERSAEEMKHDPLQQVMIEGEKGKTLMIDAGEGILVVITDIEINLGLIRIEMRRSAERVIDMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF4</accession>
    <name>F0TAF4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644257.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0655"/>
    <dbReference type="EnsemblBacteria" id="ADZ08906">
        <property type="protein sequence ID" value="ADZ08906"/>
        <property type="gene ID" value="Metbo_0655"/>
    </dbReference>
    <dbReference type="GeneID" id="25394714"/>
    <dbReference type="KEGG" id="mel:Metbo_0655"/>
    <dbReference type="eggNOG" id="arCOG06658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_817874_0_0_2"/>
    <dbReference type="OMA" id="IMVEHEG"/>
    <dbReference type="OrthoDB" id="88468at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="411" mass="46753" checksum="17CF42227121CCBF" modified="2011-05-03" version="1">MDLPITRPSTVSYAENCDFKEMLNELTSKNHNGFIRISEGDDEGFILFKNGKEIAASYERFSRADAIKKIQIAVNKDLTLIEVFDVRESQIDFLMEINRPYILGSEAYKIIDELKMVDAPKPKPMAVPKPKLVSEAIKESEQPEEVRNETESNLINVSKPEEEEDSDPKETVHRIETVKSDSPTKLETEDLPLNNTEKVENETSNKIPEPSETIPEPEPKINKSLEPEINTDTNINSTEENNSTESSPETILSDDESSEISVEENENNEMPELESEPLDRTELLKKYGIKDIQEEDVDNILESYKGGSVSEEDIEKIELTLMNKIKKSILGIPRIKGAEVMVFLENSRELTGNVNIIIETESKGFLSRIMGDSDDMKLEKQIVDMSQIEIRKSFRKYPEIVEKFDVNVEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI4</accession>
    <name>F0TAI4_METLA</name>
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        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645407.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1834"/>
    <dbReference type="EnsemblBacteria" id="ADZ10056">
        <property type="protein sequence ID" value="ADZ10056"/>
        <property type="gene ID" value="Metbo_1834"/>
    </dbReference>
    <dbReference type="GeneID" id="10278291"/>
    <dbReference type="KEGG" id="mel:Metbo_1834"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_391111_0_0_2"/>
    <dbReference type="OMA" id="YINRATI"/>
    <dbReference type="OrthoDB" id="470at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="19"/>
    </dbReference>
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        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
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    </feature>
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        <location>
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    </feature>
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    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="438"/>
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    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="472"/>
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        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
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            <begin position="506"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="540"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="608"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="693" mass="78907" checksum="E458603A8B5D5ED7" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ6</accession>
    <name>F0TAJ6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1846</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645419.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1846"/>
    <dbReference type="EnsemblBacteria" id="ADZ10068">
        <property type="protein sequence ID" value="ADZ10068"/>
        <property type="gene ID" value="Metbo_1846"/>
    </dbReference>
    <dbReference type="GeneID" id="25394753"/>
    <dbReference type="KEGG" id="mel:Metbo_1846"/>
    <dbReference type="eggNOG" id="arCOG02327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OMA" id="TAYSYLC"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PAS_fold"/>
    </dbReference>
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        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="233"/>
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        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="347"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="420"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="482"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="679" mass="77847" checksum="77CBC259F844E4F8" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN2</accession>
    <name>F0TAN2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1863 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645455.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1883"/>
    <dbReference type="EnsemblBacteria" id="ADZ10104">
        <property type="protein sequence ID" value="ADZ10104"/>
        <property type="gene ID" value="Metbo_1883"/>
    </dbReference>
    <dbReference type="GeneID" id="10278341"/>
    <dbReference type="KEGG" id="mel:Metbo_1883"/>
    <dbReference type="eggNOG" id="arCOG06471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140379_1_0_2"/>
    <dbReference type="OMA" id="EPWASEK"/>
    <dbReference type="OrthoDB" id="98429at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036490">
        <property type="entry name" value="MTH538_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015032">
        <property type="entry name" value="MTH538_TIR-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08937">
        <property type="entry name" value="DUF1863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52206">
        <property type="entry name" value="SSF52206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1863" evidence="1">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15322" checksum="43891FC912E654F5" modified="2011-05-03" version="1">MMEEESETYSLFISQLDETTYEYDRLIGKLSSVEDFKFEDHSVPGKTSKEELEDQMKDVDVVVILSGLYSTDKSLIEREIDVAKTLQKPIIMVRPYGMENVPGKIEEVANEVIGWNAGCIIESITETLEEEEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN6</accession>
    <name>F0TAN6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645459.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1888"/>
    <dbReference type="EnsemblBacteria" id="ADZ10108">
        <property type="protein sequence ID" value="ADZ10108"/>
        <property type="gene ID" value="Metbo_1888"/>
    </dbReference>
    <dbReference type="GeneID" id="10278346"/>
    <dbReference type="KEGG" id="mel:Metbo_1888"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="WAINSYT"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="15"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="500" mass="54081" checksum="6E1334B8FDD3EE5A" modified="2011-05-03" version="1">MFNKNSPTKISQNLIGFIIAFSVFIWSFSAGIVTIALPTISQYMDINTALVSWVVVAHLLVLTSFLLIFGKIGDYVGYKNIYLGGLFLFTVGSYLSGISLGITQLIASRILQGVGSAMLLSMTPAIISKVFPHTTRGKIFGFISLATTIGLSLGYGVGGIVTEYMGWHWIFFVTVPLGAAATILTYFYLPELKTGIEYRGFDLVGSILIFLAVILLIMPIEMGSKLGWTSPLIIGGLISSGVIFLIFYQWEHNYKNPLFHICLLKQKQLNISIIAGFMTSFVLTGTIFLLPFYFELILNYDTDVAGIIILLSTLLIIVVGPMSGRLADKIGSKLPAILGALLLATTMIILTLIDPTVGEFGVVLIILALIMRSLADGIFSPANNKLVMSHSPPKMVGSVSSLLNTAKYLGLVLGVVIFETIFDSTISHSSGHMEGVTGTGAFQYSVPVETLLTGFHHAFMIGVFICVLIVAASFLAKENTDVTDGLEVEPINKDIIEPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ1</accession>
    <name>F0TAQ1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645474.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1903"/>
    <dbReference type="EnsemblBacteria" id="ADZ10123">
        <property type="protein sequence ID" value="ADZ10123"/>
        <property type="gene ID" value="Metbo_1903"/>
    </dbReference>
    <dbReference type="GeneID" id="10278361"/>
    <dbReference type="KEGG" id="mel:Metbo_1903"/>
    <dbReference type="eggNOG" id="arCOG10254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387607_0_0_2"/>
    <dbReference type="OMA" id="CMLEISK"/>
    <dbReference type="OrthoDB" id="94447at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="21066" checksum="72698B4DEA2F34CB" modified="2011-05-03" version="1">MPLLKDTEREQLRQLVKACLLEISKLKIELKKCQKESSNSATDDLKVESLKQELSEHIQRKDEEIQESIKIKEDEIELLKTMIEERDQKISELETVKIYFEAVISPPRRNLTSFQSQIYELLPFEEQDTAIHFSHLRTIGFKELSHDNLESALKNLERKGYFKSRVEDGKTFWIKIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU8</accession>
    <name>F0TAU8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0723"/>
    <dbReference type="EnsemblBacteria" id="ADZ08974">
        <property type="protein sequence ID" value="ADZ08974"/>
        <property type="gene ID" value="Metbo_0723"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0723"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_7_3_2"/>
    <dbReference type="OMA" id="ETAFFRF"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="333"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="478" mass="54267" checksum="099CBED3A33B7B8D" modified="2011-05-03" version="1">MYKLFVKRIGLIGITNFLVAMNTIILIPILTKNFSAIDYGIWVQVITTFYLVTSVANLGFPYTLIRFVSAEKEKSKIQNTFYTMAVFILIFSLFISTLIFIFSNVIASLLFSGQVLIVKILSFLIFFGTLNSLLIDFFVARSKMKRYSILLLFQTYLMLSLVSFFAVCGYGIILATVGFLISQIIFFIVMTILVYQEIGFKIPEFSKIKEYINFSIPIIPNNVSTWIVESSDRYVIAIMLGTTFLAYYSPGYTIGMALLLFFTPISIILSSILPKYYENGQMEEVMMFINYSLKYFLLIAIPALFILSLLSKPILMILTTPEIALNGYLVTPFVALSAILFGIYGIIMNLIVLEKKTKIVGSIWTIAALISLLNIIFVPIFGILAAAGITLFSYSTAFLISIGYSKKFFRFYFDYTFIFKSIFASVIISILIVLINPIGFYSLLIMIPLLILIYLIIILLLKCVTNKEINFIKEIFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX0</accession>
    <name>F0TAX0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644347.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0745"/>
    <dbReference type="EnsemblBacteria" id="ADZ08996">
        <property type="protein sequence ID" value="ADZ08996"/>
        <property type="gene ID" value="Metbo_0745"/>
    </dbReference>
    <dbReference type="GeneID" id="10277192"/>
    <dbReference type="KEGG" id="mel:Metbo_0745"/>
    <dbReference type="eggNOG" id="arCOG06505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060721_1_0_2"/>
    <dbReference type="OMA" id="FVLKSHY"/>
    <dbReference type="OrthoDB" id="82477at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046249">
        <property type="entry name" value="DUF6282"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016797">
        <property type="entry name" value="UCP021898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19799">
        <property type="entry name" value="DUF6282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021898">
        <property type="entry name" value="UCP021898"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="269" mass="30010" checksum="D8AFC6CDF42D38A4" modified="2011-05-03" version="1">MKLENKEITSDSNKHPVLKDVTDIHIHTAPDIKPRLESDIEAALNSKKEFMHSIVLKSHIEPTAGRAKIASEVTGFPVFGGVVLNESVGGLNLEAVKTCAAMDGKFVWFPTISASSVKFDMNKIEEIIHVIADNNMVLLTGHLKPEEIFKLIDMAHGQGIWKIVVNHPFAGVVSATLNEQKEMSKHAFLEHCYVTCMEKHQNLDPLNIKNSIRKVGADRCIMATDFGQKHNLSPSHGMKMYIKTMEAMGIKKSEIRRMSIGNPKNLIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA1</accession>
    <name>F0TBA1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L34e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl34e</name>
        <name evidence="6" type="ordered locus">Metbo_0801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644403.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0801"/>
    <dbReference type="EnsemblBacteria" id="ADZ09052">
        <property type="protein sequence ID" value="ADZ09052"/>
        <property type="gene ID" value="Metbo_0801"/>
    </dbReference>
    <dbReference type="GeneID" id="10277249"/>
    <dbReference type="KEGG" id="mel:Metbo_0801"/>
    <dbReference type="eggNOG" id="arCOG04168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="OMA" id="SWRRKYV"/>
    <dbReference type="OrthoDB" id="119402at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_L34Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_L34e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="PTHR10759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="88" mass="10395" checksum="6AE74732C1263E80" modified="2011-05-03" version="1">MPQGRYRSRSYKRTFKKTPGGKTVLRYKKKLPNKHVCAECGKQLHAVPRGRPYQIRKLSKSKRRPNRPYGGYLCSECARRIFKQEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB4</accession>
    <name>F0TBB4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enolase</fullName>
            <ecNumber evidence="7">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">eno</name>
        <name evidence="12" type="ordered locus">Metbo_0814</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell surface</location>
        </subcellularLocation>
        <text evidence="7">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644416.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0814"/>
    <dbReference type="EnsemblBacteria" id="ADZ09065">
        <property type="protein sequence ID" value="ADZ09065"/>
        <property type="gene ID" value="Metbo_0814"/>
    </dbReference>
    <dbReference type="GeneID" id="10277263"/>
    <dbReference type="KEGG" id="mel:Metbo_0814"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_0_1_2"/>
    <dbReference type="OMA" id="QHLGGAF"/>
    <dbReference type="OrthoDB" id="23221at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="11">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="10">
        <location>
            <begin position="131"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7 8">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="7">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="418" mass="45329" checksum="0BDBF2EF7FFD91D9" modified="2011-05-03" version="1">MDSVIEDIRVRKILDSRGNPTLEVDVVTWNGFGRAAAPSGASTGAREVVAFPEGGVDKIISEVEDIISSELIGMDAEDLNEIDLVLKEIDGTENLASIGGNTTVAVSMATAKAAASSYNMPLYRFLGGNMQTEIPFPLGNMINGGAHAGKNAPDIQEFLVVPVGAETITEAVFTNVNVHKKIRELIQTKDKSFTGGKGDEGGWAPNLTNQEAIEIQVKACETVTDETGVLVKPCLDMAASEFWNAETEEYVYAKEGVNRTTGEQVDYVSELIETYGYYYVEDPIREGDFEGFADLTKKAGKNCLICGDDLFVTNAEILAEGIEKKSGNSIIIKPNQIGTLSDTYKTVELAKNNKYVPVVSHRSGETTDDTIAHLAVAFSCPIIKTGAVSGERIAKLNELIRIEEEMTYPEMADLNKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD3</accession>
    <name>F0TBD3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644435.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09084">
        <property type="protein sequence ID" value="ADZ09084"/>
        <property type="gene ID" value="Metbo_0833"/>
    </dbReference>
    <dbReference type="GeneID" id="10277282"/>
    <dbReference type="KEGG" id="mel:Metbo_0833"/>
    <dbReference type="HOGENOM" id="CLU_2177891_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="109" mass="12199" checksum="B2577B3F4AD48277" modified="2011-05-03" version="1" precursor="true">MKIKLFGMMMAIMCLAMMGGASATKLTENYMVNDIKITVTNPSADFRCEPVYNKNYVKLLGYSIYPNTDGSVVLTYFFKGKVGERILMNQYEPGIDVPIATSVYFIPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE6</accession>
    <name>F0TBE6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645566.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10215">
        <property type="protein sequence ID" value="ADZ10215"/>
        <property type="gene ID" value="Metbo_1996"/>
    </dbReference>
    <dbReference type="GeneID" id="10278456"/>
    <dbReference type="KEGG" id="mel:Metbo_1996"/>
    <dbReference type="HOGENOM" id="CLU_2010117_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="13372" checksum="6C0490846B623A65" modified="2011-05-03" version="1">MIKLKPVIVGIILIVAITMFFPLIVLPYIVIDMLSLGIILSLLVTGYIGYKISENKTNGAIYGIIIGVISGFLMGLSVSIMYQYVAASVYVFYSVFYAIFFGIIGVIGGTIGSLFIKNSEELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF1</accession>
    <name>F0TBF1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10220">
        <property type="protein sequence ID" value="ADZ10220"/>
        <property type="gene ID" value="Metbo_2001"/>
    </dbReference>
    <dbReference type="GeneID" id="10278461"/>
    <dbReference type="KEGG" id="mel:Metbo_2001"/>
    <dbReference type="eggNOG" id="arCOG00480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904930_0_0_2"/>
    <dbReference type="OMA" id="IMNIPDR"/>
    <dbReference type="OrthoDB" id="73386at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
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        <property type="entry name" value="UBIAD1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
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            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <accession>F0TBI8</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
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    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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            <end position="1238"/>
        </location>
    </feature>
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        <location>
            <begin position="1392"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1468"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10257.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1719" mass="197101" checksum="6268BE17A6412160" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL2</accession>
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        <submittedName>
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    </protein>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.47" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0839"/>
    <dbReference type="EnsemblBacteria" id="ADZ09089">
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        <property type="gene ID" value="Metbo_0839"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:GDP-mannose metabolic process"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PTHR43715"/>
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    </dbReference>
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        <location>
            <begin position="18"/>
            <end position="282"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="45458" checksum="C81DA94DCD538616" modified="2011-05-03" version="1">MNLNKINGDVMDWKDKNILITGADGFVGSYLAEELIKNGANVYGLIQRGTQGIYSQNLVNHGIEDDLKIVEGDLTDITSLAGALDLSEPEYVFHLAAQSFIPASFKDPLKTQMINANGTSNLLEAIRIKDLDPKVVFAGSSDEYGLVISSEEQYETLKSKYGVVFPEPISIPELPIKESNPLRPMSPYATSKVYCEYLMRNYHDSFGLCTVVSRAFNHEGAGRGPMFVTSVVTQQVSDLKAGKVDSIRIGNVNALKDWSHVKDVVNGYIQLAANGVSGDVYNQGAMRTNSVLSYILLSLETAGYSIEKIETVTGEKVMKDPAVTNNDPVFGVKFVKTAVDSAILEEGLEYTAQDGGINVFTDKGKVAIEFDLERFRPSEVPISLCDNTKIRSIGANYSYTVKDIIGDQLDYLNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP7</accession>
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        </submittedName>
    </protein>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09124">
        <property type="protein sequence ID" value="ADZ09124"/>
        <property type="gene ID" value="Metbo_0875"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0875"/>
    <dbReference type="eggNOG" id="arCOG02835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907971_0_0_2"/>
    <dbReference type="OMA" id="PWLEWAN"/>
    <dbReference type="OrthoDB" id="77334at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007354">
        <property type="entry name" value="CruF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39419">
        <property type="entry name" value="PTHR39419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04240">
        <property type="entry name" value="Caroten_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
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            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
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        <location>
            <begin position="86"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
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        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="307" mass="34635" checksum="AF5660D8F3A05BF0" modified="2011-05-03" version="1">MISLLLDLLIPLFAIFIAVFSKRLDINQNFYIKLFFGLFVISISSAIISYVIPSPILGLVGSFYMWLAFGFVLLHSSKVLGNKKTLIFFIIAYLFGFTFEAIGVKYGGIFGMPYYYQNIPTFFFGLVPISTPISWSIIIYFSYTLTNLFLFGFGGKKPRKTDDRGYFWVMIVILSAIGGFVALNLDMILDPVAVAPQVGGWVWSGGGPYFGIPIGNFIGWFMVSAVAILIFRYYESVSSKTNPKYELNIPLNSYIVLLYFIYFLENAVRALTLGKVEYILIGATTMMPFVLVSLLVLLLLWKNENHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR7</accession>
    <name>F0TBR7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0895"/>
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        <property type="protein sequence ID" value="ADZ09144"/>
        <property type="gene ID" value="Metbo_0895"/>
    </dbReference>
    <dbReference type="GeneID" id="10277344"/>
    <dbReference type="KEGG" id="mel:Metbo_0895"/>
    <dbReference type="eggNOG" id="arCOG02856">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134822_0_0_2"/>
    <dbReference type="OMA" id="AFIPDMA"/>
    <dbReference type="OrthoDB" id="111097at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="159" mass="17947" checksum="51F9B835DBDAA26C" modified="2011-05-03" version="1">MPDWVTHIAVAWIICRLLSFKYDEFNAANTMLVITGALIPDFSKFILVFKFFGLDLSEYLSIIHIPTGSVIVAGMMSMLFPEKKKAFLFFGLGIITHYSLDIILEHVSGGIYLLFPFSWWQWQLQITNSSDYLITLLALCLAGLVYLVGREIDANKGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV0</accession>
    <name>F0TBV0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Methanomada group</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645643.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2073"/>
    <dbReference type="EnsemblBacteria" id="ADZ10292">
        <property type="protein sequence ID" value="ADZ10292"/>
        <property type="gene ID" value="Metbo_2073"/>
    </dbReference>
    <dbReference type="GeneID" id="10278535"/>
    <dbReference type="KEGG" id="mel:Metbo_2073"/>
    <dbReference type="eggNOG" id="arCOG10260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901931_0_0_2"/>
    <dbReference type="OMA" id="RIRYNLH"/>
    <dbReference type="OrthoDB" id="122089at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="15851" checksum="9E5B6982F6FC7639" modified="2011-05-03" version="1">MSPKKYRQQLLDLEIDGFEIDTSTINNTMDTLNKLNELEKILSKIRYNVRADIRNIRLDYMGKLQEVDKSVNEKQGLLRRKKSVSQVIKEKKVLTKERNLTIATYAVVENTIDDYLDQIEQSKFYIKSSIQHRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW3</accession>
    <name>F0TBW3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0235 protein Metbo_2086</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2086"/>
    <dbReference type="EnsemblBacteria" id="ADZ10305">
        <property type="protein sequence ID" value="ADZ10305"/>
        <property type="gene ID" value="Metbo_2086"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2086"/>
    <dbReference type="eggNOG" id="arCOG04058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130694_6_1_2"/>
    <dbReference type="OMA" id="HEEGWER"/>
    <dbReference type="OrthoDB" id="123783at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005228">
        <property type="entry name" value="CHP00251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="SSF69786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00251">
        <property type="entry name" value="TIGR00251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="11616" checksum="3F0DBACE43C12ADA" modified="2011-05-03" version="1">MVEMEAITTTKDGVLLNIEVGTKSDNFRITGYNDWRKSFEIKIKAVPQKGKANKEIILEFAKLTNKRVEIISGHKSHRKTLKIYDINEEDLLKLIEQEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ3</accession>
    <name>F0TBZ3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name evidence="2" type="ordered locus">Metbo_2116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645686.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2116"/>
    <dbReference type="EnsemblBacteria" id="ADZ10335">
        <property type="protein sequence ID" value="ADZ10335"/>
        <property type="gene ID" value="Metbo_2116"/>
    </dbReference>
    <dbReference type="GeneID" id="10278579"/>
    <dbReference type="KEGG" id="mel:Metbo_2116"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="OrthoDB" id="90817at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="217" mass="24113" checksum="AC6F7B4C8B214EB5" modified="2011-05-03" version="1">MKVGIIRFPGSNCDRDVFHVLELVGAKPEYVWWNTRDLSDYEAIVIPGGFSYGDYLRAGAIAAITPIVDGIKDCVSEGKPVLGICNGAQILAEVGLVPGVFTENENAKFICEWTDLKVESVKTPFTRLYKKNEIIRMPIAHAEGRYYTENIQELRDNDQIVLSFESENPNGSMEAITGVCDQEGLVCAVMPHPERASESILGSDDGRKFFEGIMKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC19</accession>
    <name>F0TC19_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0921</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644521.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0921"/>
    <dbReference type="EnsemblBacteria" id="ADZ09170">
        <property type="protein sequence ID" value="ADZ09170"/>
        <property type="gene ID" value="Metbo_0921"/>
    </dbReference>
    <dbReference type="GeneID" id="10277370"/>
    <dbReference type="KEGG" id="mel:Metbo_0921"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444562_0_0_2"/>
    <dbReference type="OrthoDB" id="128181at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="658" mass="68400" checksum="915092A5581CC4CC" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC32</accession>
    <name>F0TC32_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09183">
        <property type="protein sequence ID" value="ADZ09183"/>
        <property type="gene ID" value="Metbo_0936"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0936"/>
    <dbReference type="HOGENOM" id="CLU_3227813_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="5103" checksum="99671B2DB3456A7E" modified="2011-05-03" version="1">MIMYVGIYNVVVYKMGACIEKILEFDKYNKINIWGFKSKHPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC59</accession>
    <name>F0TC59_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644561.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0963"/>
    <dbReference type="EnsemblBacteria" id="ADZ09210">
        <property type="protein sequence ID" value="ADZ09210"/>
        <property type="gene ID" value="Metbo_0963"/>
    </dbReference>
    <dbReference type="GeneID" id="10277412"/>
    <dbReference type="KEGG" id="mel:Metbo_0963"/>
    <dbReference type="eggNOG" id="arCOG06454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815375_0_0_2"/>
    <dbReference type="OMA" id="VSVKPSM"/>
    <dbReference type="OrthoDB" id="27690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase NYN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="342" mass="38773" checksum="150468E9FE195D23" modified="2011-05-03" version="1">MKVIIDASNVAHFGKEKEGNKPRLDYLLKSVEALEKLGYEPFPIADASLRHEIDEKEKFNAMLDEGKVQQVPSGTTADHFILKLAFEEDCKILSNDNFREFNDEFHDINNRRIPFTFVEDAISIGSSSKPKKVKNILQKISTQMLNDFEKKGLESYKLKKNKKLSGIAVAKEAIDRITKTTENTFDSKIEDIFMKIPLFDKVMGMVEDAEKTSDFIIFVLVSPKDYKDTVRNAGNIAVTVGDRLKLDHAPLVAIRNDLFTKPGTFELNIIYSDEILEESPYNVNITINDHDYSFVKKNSRNIASTVAARLGTWKFPIVSVKPSMLMEKPGHFDITLEKGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC78</accession>
    <name>F0TC78_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">(5-formylfuran-3-yl)methyl phosphate synthase</fullName>
            <ecNumber evidence="5">4.2.3.153</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase</fullName>
            <shortName evidence="5">4-HFC-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfnB</name>
        <name evidence="7" type="ordered locus">Metbo_0982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="EC" id="4.2.3.153"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MfnB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.153" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644580.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0982"/>
    <dbReference type="EnsemblBacteria" id="ADZ09229">
        <property type="protein sequence ID" value="ADZ09229"/>
        <property type="gene ID" value="Metbo_0982"/>
    </dbReference>
    <dbReference type="GeneID" id="10277431"/>
    <dbReference type="KEGG" id="mel:Metbo_0982"/>
    <dbReference type="eggNOG" id="arCOG04482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068659_0_0_2"/>
    <dbReference type="OMA" id="NFPWVIR"/>
    <dbReference type="OrthoDB" id="71837at2157"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00681">
        <property type="entry name" value="MfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007565">
        <property type="entry name" value="4HFCP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035081">
        <property type="entry name" value="4HFCP_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04476">
        <property type="entry name" value="4HFCP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015957">
        <property type="entry name" value="UCP015957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5 6">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015957-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="237" mass="25245" checksum="53511DA791F5AA17" modified="2011-05-03" version="1">MLLLISPINAEEALESIEGGADIVDVKNPKEGSLGANFPWVIKNIREITPEGMHVSATLGDVPYKPGTVSLAAAGAVVSGADYIKVGLYGTKNYDEALEVMENVVKSVREFDEDALVVASGYADAHRVGAVDPMEIPRVAADAGADLAMVDTAVKDGKTLFDFMDVEKLQKFNDSIHDYGLKSALAGSVKKDQLKLLYDIGCDVVGIRGAACTGGDRNNGSIHRTAVKELKDMIENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC94</accession>
    <name>F0TC94_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645712.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2144"/>
    <dbReference type="EnsemblBacteria" id="ADZ10361">
        <property type="protein sequence ID" value="ADZ10361"/>
        <property type="gene ID" value="Metbo_2144"/>
    </dbReference>
    <dbReference type="GeneID" id="10278607"/>
    <dbReference type="KEGG" id="mel:Metbo_2144"/>
    <dbReference type="eggNOG" id="arCOG02772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076047_0_0_2"/>
    <dbReference type="OMA" id="VCLVPHV"/>
    <dbReference type="OrthoDB" id="65742at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="26901" checksum="516031A692D6F12C" modified="2011-05-03" version="1">MKKHKIRLRIYILVALVLTLPILINVYSPILMVWGIRNGIITASFLIFAAWFMLSLFMGRSVSCAYTCPYGALQEILGIHVSKKKPKSEKANKIRYFISVIFLLLVSFLIFNLGVAKNIDLFASNDDLQLIILITISIITISLLSILFGSRAFCRYLCPQGVFLTIGSKIGRKIKIPSLQLKSTPNNCSNCKLCDKSCPMGLDVNQMVTNSTLNDLNCILCGECIEKCPKDAINYSFTAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE4</accession>
    <name>F0TCE4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase vitamin-B12 independent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645762.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2197"/>
    <dbReference type="EnsemblBacteria" id="ADZ10411">
        <property type="protein sequence ID" value="ADZ10411"/>
        <property type="gene ID" value="Metbo_2197"/>
    </dbReference>
    <dbReference type="GeneID" id="10278660"/>
    <dbReference type="KEGG" id="mel:Metbo_2197"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="OMA" id="DPDCGMR"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="324" mass="35315" checksum="C70B3670356EE7AA" modified="2011-05-03" version="1">MLTTVVGSYPVVETKTETFGTKISRLFGSYDEFKPSLEMAVKDQVLAGVDIVSDGQVRGDMVEIFAKKLPGMTVEDDVPGINGKIRALNGSIGAADMKLAIKTAENISEDFKNSEPLMDGNHLNHSYKGVKGIVTGPTTLVLSSRVLDFYKKDNKSKIIMDMAQALKKEAKELEAAGAGMIQIDEPFLSTGIADLKVAKEAIKTISTDLKVPVAMHVCGDVEEVLGDLLKFPVQIIDCEFAGVDSNIEALKTEYRGSKKIGFGCLDTKTEVVETVEQVSNLVKKGIDIVGKENMMIDPDCGMRMLSREIAQLKLKKMKETVSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF2</accession>
    <name>F0TCF2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2205</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645770.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2205"/>
    <dbReference type="EnsemblBacteria" id="ADZ10419">
        <property type="protein sequence ID" value="ADZ10419"/>
        <property type="gene ID" value="Metbo_2205"/>
    </dbReference>
    <dbReference type="GeneID" id="10278669"/>
    <dbReference type="KEGG" id="mel:Metbo_2205"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_21_1_2"/>
    <dbReference type="OMA" id="EHKDGQR"/>
    <dbReference type="OrthoDB" id="34464at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="4"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="35"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="217"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="4"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="529" mass="59446" checksum="95DA07B4667AC379" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG9</accession>
    <name>F0TCG9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0999</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="7 8">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644597.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0999"/>
    <dbReference type="EnsemblBacteria" id="ADZ09246">
        <property type="protein sequence ID" value="ADZ09246"/>
        <property type="gene ID" value="Metbo_0999"/>
    </dbReference>
    <dbReference type="GeneID" id="10277448"/>
    <dbReference type="KEGG" id="mel:Metbo_0999"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_3_3_2"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="5785" checksum="3659B41BCFC2C3E1" modified="2011-05-03" version="1">MKRYKCKVCGYIYDPEVGDKKGKVEPGTAFEDLKSNWHCPSCGAGTNRFVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI2</accession>
    <name>F0TCI2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName evidence="1">LP guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofC</name>
        <name evidence="2" type="ordered locus">Metbo_1012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644610.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1012"/>
    <dbReference type="EnsemblBacteria" id="ADZ09259">
        <property type="protein sequence ID" value="ADZ09259"/>
        <property type="gene ID" value="Metbo_1012"/>
    </dbReference>
    <dbReference type="GeneID" id="10277461"/>
    <dbReference type="KEGG" id="mel:Metbo_1012"/>
    <dbReference type="eggNOG" id="arCOG04472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076569_2_0_2"/>
    <dbReference type="OMA" id="FRVDYHG"/>
    <dbReference type="OrthoDB" id="67356at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="PTHR40392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25256" checksum="92A370980C98A9AA" modified="2011-05-03" version="1">MKKTFAIIPVSRFTHAKTRLSPTLTPLQRENLLKSMLKDVTNALRNLVENIVVISSDEDVLNYVKNLEVIPIKEEGSTDLNGALKQAIKWCSSQADQVLIIPSDVPLIRPEYVAEMLKLGEKNDMVIAPAKGGGTNAMLCPVNGMEMLFGDCSFFEHIKIAESKNLMVKVYDSFYMALDVNTAEDLGEIMLHGSGTEAQKFLSKEGLVVVPIHGKERLQIERREINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ5</accession>
    <name>F0TCJ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase MtaA/CmuA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644623.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1025"/>
    <dbReference type="EnsemblBacteria" id="ADZ09272">
        <property type="protein sequence ID" value="ADZ09272"/>
        <property type="gene ID" value="Metbo_1025"/>
    </dbReference>
    <dbReference type="GeneID" id="10277474"/>
    <dbReference type="KEGG" id="mel:Metbo_1025"/>
    <dbReference type="eggNOG" id="arCOG03324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_2_1_2"/>
    <dbReference type="OMA" id="YERYCAP"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03307">
        <property type="entry name" value="Mta_CmuA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006360">
        <property type="entry name" value="Mtase_MtaA_CmuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01463">
        <property type="entry name" value="mtaA_cmuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Uroporphyrinogen_deCOase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="353" mass="38339" checksum="E4D709857C05160A" modified="2011-05-03" version="1">MNLKNNVLKVLSGEKTDLTPVISVTQLGVVEAMEQTDAFWPEAHEDADKMATLGSSLYELVGLECARIPFCLTVEAEALGCEVDLGNEEKTPQIRSTPFKTASEIEVPANFLSNGRIPVVLEAIEKLKQKYGDTLPIIVGITGPFTLTGHLLGVENLVRYMKTKPDEIETAIDNSLDACMDYAEAISKVGPDIICVAEPTAAPELIDPLQFKSMVKPALEDLANFIKTKKVLHICGSTQPIIKDMATIGYDGISVEEKVDIKKAKEELEKGSNVMKVGGKSMPRGSSSISKIIGNISTTQTLFRGTEEEIKNEVKMVLDAGIDILAPSCGLAPRSPLKNIEAMINARNKFFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK2</accession>
    <name>F0TCK2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644630.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09279">
        <property type="protein sequence ID" value="ADZ09279"/>
        <property type="gene ID" value="Metbo_1032"/>
    </dbReference>
    <dbReference type="GeneID" id="10277481"/>
    <dbReference type="KEGG" id="mel:Metbo_1032"/>
    <dbReference type="HOGENOM" id="CLU_1792139_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="15534" checksum="0948955819298524" modified="2011-05-03" version="1" precursor="true">MKKMISIILGIVLIGLVILIIIPSSSHVENGPVTLVKEADIGGDGTQIHFVTVSSDDNLKVSITNVTKISDTTNVAEIKVFALKIEGANGQLPESYGNNVIKKNKFGNLKTSCFEGNITYDKGVKSFGFVTNNVKAHVTILKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK4</accession>
    <name>F0TCK4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1034</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644632.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1034"/>
    <dbReference type="EnsemblBacteria" id="ADZ09281">
        <property type="protein sequence ID" value="ADZ09281"/>
        <property type="gene ID" value="Metbo_1034"/>
    </dbReference>
    <dbReference type="GeneID" id="10277483"/>
    <dbReference type="KEGG" id="mel:Metbo_1034"/>
    <dbReference type="eggNOG" id="arCOG04145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="OMA" id="IAWIFFM"/>
    <dbReference type="OrthoDB" id="13996at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="494" mass="55183" checksum="0D72A07D3C62551C" modified="2011-05-03" version="1">MHSMSKFRRSEIYSLLHYTGYISVLLGIVMLVPIIVAFIYHEPHYVLPFIYSSITSLVFGVVLYKCFSSGSEISLKVAMLFSTLIWLIGSAIGALPFYLSGDLSYLNSYFEAMSGFTTTGFSMYSNLDTVSYTMDFWKGFMQWLGGIGIIVMALTVLSSSNVSIMRLYSAEGRDERLVPSIKHTTRIILYIYLAYTAVSILLFIVAGMPIFDSIFYAFTALSTGGFAMQNASIGYYHSIWIEIVAMIIMIMGATNFALHYTVLKGNWKEYFKDIETRVSWPLIILGTIIGTIFLFNTSVYGHDMLISLRYSIFQVVSALTTTGLQTATPSDITSQWEGLGIFILTLLMIVGAGACSTGGGIKWIRIGILIKGMWWEIKSLILPESAVISKKIHHINDVKIDTKLLKITGLFVFSYLVIYFVSVIIVLFYYPNVSQTLFEVASALSNVGLGSGLITATSPFVVKIVFIIDFWIGRLEIWPILLLVALLIQNSIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL4</accession>
    <name>F0TCL4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644642.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1044"/>
    <dbReference type="EnsemblBacteria" id="ADZ09291">
        <property type="protein sequence ID" value="ADZ09291"/>
        <property type="gene ID" value="Metbo_1044"/>
    </dbReference>
    <dbReference type="GeneID" id="10277493"/>
    <dbReference type="KEGG" id="mel:Metbo_1044"/>
    <dbReference type="eggNOG" id="arCOG02386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="15328" checksum="E5853A1980B419FA" modified="2011-05-03" version="1">MSIEPIKVLMIEDNRGDYQLILKLLEKSENAQFELVHSPRLKSGVELLENNSFDVILLDLGLPDSNGLDSFNVILKKHPEIPTIILTGLANEETGIQAIKYGAQDYLVKGEFNGKLLVRAIQYAIERKKLEGLFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN5</accession>
    <name>F0TCN5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644663.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1066"/>
    <dbReference type="EnsemblBacteria" id="ADZ09312">
        <property type="protein sequence ID" value="ADZ09312"/>
        <property type="gene ID" value="Metbo_1066"/>
    </dbReference>
    <dbReference type="GeneID" id="10277515"/>
    <dbReference type="KEGG" id="mel:Metbo_1066"/>
    <dbReference type="HOGENOM" id="CLU_2802257_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="67" mass="7543" checksum="0EF62D437565904D" modified="2011-05-03" version="1">MILPRYEYFLCNCEKGDIILTKNGFGKVRSNKRKGNDRKAVYTSIKGCNKCGSTIDSHGTDLKKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT3</accession>
    <name>F0TCT3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HEAT domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645824.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2259"/>
    <dbReference type="EnsemblBacteria" id="ADZ10473">
        <property type="protein sequence ID" value="ADZ10473"/>
        <property type="gene ID" value="Metbo_2259"/>
    </dbReference>
    <dbReference type="GeneID" id="10278725"/>
    <dbReference type="KEGG" id="mel:Metbo_2259"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1106326_0_0_2"/>
    <dbReference type="OrthoDB" id="96576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="269" mass="30893" checksum="4B3CB7F4B396FE37" modified="2011-05-03" version="1">MGFYDLSKDERSKLVKQIHDEIEESILNAKFDGDGNSSAPEILVQYASNEDGYIRKAAYNAVAKIYLENNDLHKKILTIMAELLNNSNPKVRQTAVYVLGEIGTKDITDILVIFETALTDEHHSVRNAVIGALKRMGQRNPKQTFKFASKHIQSKNPMIRREIVHGIELRGRTHPEEVMPILKKLEYEEDKAVKQMIIHVIGQISYKKGCLEVVVRDLNDWSNKGLVLEAFKEIVEVHENYKFATRTPEQAKNYIKKNLETKNLNLEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T2</accession>
    <name>F0T5T2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644676.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1080"/>
    <dbReference type="EnsemblBacteria" id="ADZ09325">
        <property type="protein sequence ID" value="ADZ09325"/>
        <property type="gene ID" value="Metbo_1080"/>
    </dbReference>
    <dbReference type="GeneID" id="10277529"/>
    <dbReference type="KEGG" id="mel:Metbo_1080"/>
    <dbReference type="eggNOG" id="arCOG14003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639976_0_0_2"/>
    <dbReference type="OMA" id="KELWWIR"/>
    <dbReference type="OrthoDB" id="127502at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="19044" checksum="F533020C21AD970D" modified="2011-05-03" version="1">MTDDQERIEIIEKLLSYKLALEKSRPELLKELGKYYSSENNGNPHDSHDEFKDVEFMSFTDASEAYILTQIKVVDSMPRVINEHIFRLNVGDDIDDVLESFEIEVYGVKKDIYKNLTEWEDLLDHVPDERIQELTRNPKGHGDKILKELWWIRKFEDQNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V0</accession>
    <name>F0T5V0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644694.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1098"/>
    <dbReference type="EnsemblBacteria" id="ADZ09343">
        <property type="protein sequence ID" value="ADZ09343"/>
        <property type="gene ID" value="Metbo_1098"/>
    </dbReference>
    <dbReference type="GeneID" id="10277548"/>
    <dbReference type="KEGG" id="mel:Metbo_1098"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="RYPRFVG"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="15"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="474" mass="51308" checksum="647A4BC9ACB6DD10" modified="2011-05-03" version="1">MEADTHNDKNLKLTALIVVCIGSFLIPFMGSSLNIVLPVIQNELSVNLIILSWIPTVFVLANAATILPLGRLGDIIGRKKVYAYGMVIYTVASLLAGLSTSGIMLVCFSFMQGIGCAMIFASAVALLSSVYPSNQRGEALGAYITAVYIGLFLGPLLGGFLANNIGWRSIFLFNVPVGIISLILIKFKLHGDWKGYDGEKFEKSGSVIYIIALLAILYGISSFQTNLGKLILLAGIFGLAIFILKERGSKNPILRLNIFKKRVSSFSALALLLMNIATTSMWAILSLYFQDILSLSPLTTAMILSVQPLMVALLSVPVGRLADRVENRTFSIAGMILVTLGLIIFSTLQPQTELYIPIIGLILVGIGLGLFNSPTTNKFMGSIEGRDYGMAGATLSTMIYVGQTLSLSIMLFIFSIYIGNVQINNSNFSLYLLSLKTAFTAFAIITGLGTIITLLIGKNNLKKIKSDQYTKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V2</accession>
    <name>F0T5V2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644696.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1100"/>
    <dbReference type="EnsemblBacteria" id="ADZ09345">
        <property type="protein sequence ID" value="ADZ09345"/>
        <property type="gene ID" value="Metbo_1100"/>
    </dbReference>
    <dbReference type="GeneID" id="10277550"/>
    <dbReference type="KEGG" id="mel:Metbo_1100"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="SLMDITH"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
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        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="467" mass="53759" checksum="F837917D9AFEDD13" modified="2011-05-03" version="1">MEPIRILLVEDVELDAELTERELKRTNLNYVSKRVEEESDFIDLLKSFKPDIILADHSLPNFDGVTALKITKEKCPETPFIFVSGKIGEDFAVEMLKEGATDYVLKSNLPKLGHAVQRALKEFNEQVELKFAQEALLESENKYRTLFEKNKHPIIVFDDKGNFTDSNEAALMFMETNKEHLLTKNLSDVILYSDDVETIGSEEFWNKKDNLEVTFEIKGKLKILELTLTPVYLNTETIVFGVGTDITKRKIDEEKILNSLKEKELLLREIHHRVKNNLQIISTLLTLQSSQNNNVNVDDLYRESQNRIQSISLIHENLYHSKDLANINFEIYAKGLIMDLFDSYGMDSQKVRLNLDIDNITMGIETAIPCGLIINELVSNSLKHGFSDFKTGEVNVKLHRIQDGKFSLVISDTGRPFPENINLVNNETLGLELIKNLVNQLDAQLKFDRDKKEFNIIFKELEYKERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X8</accession>
    <name>F0T5X8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644722.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09371">
        <property type="protein sequence ID" value="ADZ09371"/>
        <property type="gene ID" value="Metbo_1127"/>
    </dbReference>
    <dbReference type="GeneID" id="24964534"/>
    <dbReference type="KEGG" id="mel:Metbo_1127"/>
    <dbReference type="eggNOG" id="arCOG11271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056068_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="13438" checksum="84999B883B89C1C5" modified="2011-05-03" version="1">MNFKNLNLIIGLIIAIFLAYIIPNTIGSLIAVFLGAMYVGYMIEKNYKEGIFNGAILGVLYAIFAAIATYYIMNYHFVSVSELTTWIFSLIILYVVCGVLGGIFGTFIKNLKILSFDKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T633</accession>
    <name>F0T633_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1722 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645891.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2327"/>
    <dbReference type="EnsemblBacteria" id="ADZ10540">
        <property type="protein sequence ID" value="ADZ10540"/>
        <property type="gene ID" value="Metbo_2327"/>
    </dbReference>
    <dbReference type="GeneID" id="10278793"/>
    <dbReference type="KEGG" id="mel:Metbo_2327"/>
    <dbReference type="eggNOG" id="arCOG04848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076318_0_1_2"/>
    <dbReference type="OMA" id="HEWEDLE"/>
    <dbReference type="OrthoDB" id="57208at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007553">
        <property type="entry name" value="2-thiour_desulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013560">
        <property type="entry name" value="DUF1722"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017087">
        <property type="entry name" value="UCP037004"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04463">
        <property type="entry name" value="2-thiour_desulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08349">
        <property type="entry name" value="DUF1722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037004">
        <property type="entry name" value="UCP037004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1722" evidence="1">
        <location>
            <begin position="189"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35906" checksum="952661CB29533CA3" modified="2011-05-03" version="1">MDFEKPRIVVSKCIEFAPCRYNGLMIKSSIVEKLKDYADFLPVCPEVGMGLGVPRDPIRVVDSGGGLELFQPKTGKILTEEMNKFSQTFLESISDVDGFILKNKSPSCGVKAINIYPSFEDSRPQKDGIGLFAAHVSKVFPELPLEDEGRLRNLIIRENFLTSIFALAKFRRAISGDFNDLITFHTKNKLFLMSRSPYYLKLMGQILANNDKKAIDNIKEEYQGLLFRSLAEDSNPSLNVNVMMHAMGYFSKGIKHDEKTLFLNTLNDYKNGRIPLLVCLKMLELWIIRFENDYLAEQTFFKPYPHDLMQITFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T670</accession>
    <name>F0T670_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative (R)-citramalate synthase CimA</fullName>
            <ecNumber evidence="5">2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cimA</name>
        <name evidence="8" type="ordered locus">Metbo_2364</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645928.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2364"/>
    <dbReference type="EnsemblBacteria" id="ADZ10577">
        <property type="protein sequence ID" value="ADZ10577"/>
        <property type="gene ID" value="Metbo_2364"/>
    </dbReference>
    <dbReference type="GeneID" id="10278830"/>
    <dbReference type="KEGG" id="mel:Metbo_2364"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="SNMFAHE"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01028">
        <property type="entry name" value="CimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024890">
        <property type="entry name" value="Citramalate_synthase_CimA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="501" mass="53807" checksum="8A467318B50E9905" modified="2011-05-03" version="1">MKARIFDTTLRDGEQTPGVSLTPDQKLRIAVKLDNLGVDVIEAGSAITSSGEREGIKSIVSEGLSAEICSFARAMRIDVDAALECGVDSVHLVVPTSDLHIEHKLRKTREEVKATAIDAVEYAVDNGLLVELSAEDATRSDYNYLKQVFNEGIEAGARRICACDTVGMLTPEKAYEFYGGLTQLGVPLSAHCHNDFGLAVANTLSGLRGGASQAHVTVNGIGERAGNASLEEVVVSLYSLYNSKTNINIEMLYETSKTVARMTGIYLQPNKAIVGENAFAHESGIHSDGVIKKAETYEPITPELVGHKRRFVMGKHVGSHIIRKRIKEMGLRVDDARFEQIFQRVKALGDMGKCVTDVDLQAIAEDALGVLAEKPVELEELTIVSGNKVTPTASVKINVDGSEKVEAGVGVGPVDAAIVAIKKSIMDVADIQLEEYHVDAITGGTDALIDVVVKLKNGDNIVTARSTQPDIIMASVEAVLGGVNKIVSDQKIKESEEKDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E2</accession>
    <name>F0T6E2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cysteine desulfurase IscS</fullName>
            <ecNumber evidence="7">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">iscS</name>
        <name evidence="10" type="ordered locus">Metbo_1214</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In Archaea the pyridoxal phosphate cofactor is not covalently bound to Lys but ligated by other amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1214"/>
    <dbReference type="EnsemblBacteria" id="ADZ09457">
        <property type="protein sequence ID" value="ADZ09457"/>
        <property type="gene ID" value="Metbo_1214"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1214"/>
    <dbReference type="eggNOG" id="arCOG00066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_2"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017772">
        <property type="entry name" value="Cys_deSase_NifS_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03402">
        <property type="entry name" value="FeS_nifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="9">
        <location>
            <begin position="2"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="7">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="7">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="7">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="7">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared with IscU</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="387" mass="42059" checksum="5479B7C478C97142" modified="2011-05-03" version="1">MIYMDHSATSPVDPEVFEAMKPYFVDSFGNASTLYSLGREGKQAMEAARIEVASLINAEPKEIIFTSGGTESDNLAIKGTAYKLKNKGNHIITTNIEHPAVDETCKYLEKNGFKVTYLPVGEDGIIKVDDLKAAITNETILITVMHANNEIGTIQPIEEIGKIASEKKIYFHTDAVQTVGKIPVDVKKMNVDMLSLSSHKLYGPKGVGALYIKKGVRIEPLLHGGGHERGLRPGTENVAGIVGLGKACSIAKVSQETDAQKLTNLRNKLVDGILNSIEDSYLNGDRTKRLPNNANFRFTGIEGESLILRLDTKGIAASTGSACSSTKLEASHVLTAIGLKEVEAHGSLRISLGHENTEEDIEYAIKAIKEVVEQLRQMSPLWCGIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I0</accession>
    <name>F0T6I0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Triosephosphate isomerase</fullName>
            <shortName evidence="2">TIM</shortName>
            <shortName evidence="2">TPI</shortName>
            <ecNumber evidence="2 3">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tpiA</name>
        <name evidence="4" type="ordered locus">Metbo_2401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="2 3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645965.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2401"/>
    <dbReference type="EnsemblBacteria" id="ADZ10614">
        <property type="protein sequence ID" value="ADZ10614"/>
        <property type="gene ID" value="Metbo_2401"/>
    </dbReference>
    <dbReference type="GeneID" id="10278873"/>
    <dbReference type="KEGG" id="mel:Metbo_2401"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OMA" id="IPVYAQH"/>
    <dbReference type="OrthoDB" id="60670at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="2">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="23674" checksum="A163CC217A6851D4" modified="2011-05-03" version="1">MNNTKRTPIVILNFKTYLESTGINAVNLATASEMVAEETGINMVVAPQSSDIYQLSNKVNIPVFAQHIDAVDAGGHTGSTLIECVKEAGADGSLINHSEQRMKLADIDVAVKKLAQKNMVSVLCTNNIETSAAVATLKPDFVAIEPPELIGSGIPVSKAEPEIVEGTVEVIHKIDPEISVLCGAGISTGDDMKAAMDLGADGVLLASGIIKADDPKEALLNLVSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J6</accession>
    <name>F0T6J6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2417</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in the regulation of nitrogen fixation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645981.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2417"/>
    <dbReference type="EnsemblBacteria" id="ADZ10630">
        <property type="protein sequence ID" value="ADZ10630"/>
        <property type="gene ID" value="Metbo_2417"/>
    </dbReference>
    <dbReference type="GeneID" id="10278889"/>
    <dbReference type="KEGG" id="mel:Metbo_2417"/>
    <dbReference type="eggNOG" id="arCOG02306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_1_2"/>
    <dbReference type="OMA" id="LMIRTIV"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="11819" checksum="01D6A8309E1DCE6E" modified="2011-05-03" version="1">MKMIRAIIRPERVENVVDALDKAGFVAMTKMEVIGRGKQKGIQLENIYYDEIPKTMLMLVVEDEKADKIVEIVTDTSYTGNFGDGKIFVSPVDEAYTVRTRSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M3</accession>
    <name>F0T6M3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0107 protein Metbo_0003</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0107 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643607.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0003"/>
    <dbReference type="EnsemblBacteria" id="ADZ08256">
        <property type="protein sequence ID" value="ADZ08256"/>
        <property type="gene ID" value="Metbo_0003"/>
    </dbReference>
    <dbReference type="GeneID" id="10276425"/>
    <dbReference type="KEGG" id="mel:Metbo_0003"/>
    <dbReference type="eggNOG" id="arCOG04279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141583_2_0_2"/>
    <dbReference type="OMA" id="CEPIVAT"/>
    <dbReference type="OrthoDB" id="108624at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00078">
        <property type="entry name" value="UPF0107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="AcnX_sub2_archaea_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="AcnX_sub2_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="AcnX_swivel_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="DUF126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF126" evidence="3">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="14439" checksum="4F9D1F9740CC6388" modified="2011-05-03" version="1">MDPKIINCRKISKGITSGEVIITRDSLSFLGGVNPDTGEIIDSKHELFGKNIKNKILVMPSGKGSTVGSYVLYQMAKNSTAPLAVVAIEGEPIIATGAIMAGIPMVDRPEIDIFDLLHDGQVVEVNADEGYLKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R1</accession>
    <name>F0T6R1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643645.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0041"/>
    <dbReference type="EnsemblBacteria" id="ADZ08294">
        <property type="protein sequence ID" value="ADZ08294"/>
        <property type="gene ID" value="Metbo_0041"/>
    </dbReference>
    <dbReference type="GeneID" id="10276463"/>
    <dbReference type="KEGG" id="mel:Metbo_0041"/>
    <dbReference type="eggNOG" id="arCOG10170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801705_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15881" checksum="D59BDE9D2E33F4FF" modified="2011-05-03" version="1">MIKKEECGIFLCYDALLALLPIIIILAAVTNIQIDPSGSNLELVLFHQAQDTLDLMSISANPLEPSTLEQISSSISDNKLESAHKIGNNWLKNRVENRKYRLVEVNQRNGQEICSSPGIDNTKIVAVAVKSYKGHVYKLYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T3</accession>
    <name>F0T6T3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">albA</name>
        <name evidence="9" type="ordered locus">Metbo_0063</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643667.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0063"/>
    <dbReference type="EnsemblBacteria" id="ADZ08316">
        <property type="protein sequence ID" value="ADZ08316"/>
        <property type="gene ID" value="Metbo_0063"/>
    </dbReference>
    <dbReference type="GeneID" id="10276486"/>
    <dbReference type="KEGG" id="mel:Metbo_0063"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OMA" id="QFNEGAK"/>
    <dbReference type="OrthoDB" id="111461at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 7" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Alba" evidence="8">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10439" checksum="B2010092F3F6CB41" modified="2011-05-03" version="1">MPEENIVYIGNKPVMNYVLAVVTQMNGGLSEVVLKARGRAISRAVDVAEIVRNRFITDAQVESIDISTEEIMNNEGTNTNVSAIEIQLSRDDTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T9</accession>
    <name>F0T6T9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0069"/>
    <dbReference type="EnsemblBacteria" id="ADZ08322">
        <property type="protein sequence ID" value="ADZ08322"/>
        <property type="gene ID" value="Metbo_0069"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0069"/>
    <dbReference type="eggNOG" id="arCOG04365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803466_0_0_2"/>
    <dbReference type="OMA" id="LFEHHTR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014846">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08735">
        <property type="entry name" value="DUF1786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029129">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="336" mass="37189" checksum="1E476D49D5D897F7" modified="2011-05-03" version="1">MAVDIGVGTQDIMLYDSEDTIENSFKMVMPSPTRIIAEKIRNHHNDIFIKGSTMGGGPINKAIKNHLDRGYKVIMTESAAITVRDDLDRVRQFGITIIPDNEKRPELGSVELKDIDLDAIEGAFSKFDVEMDFDELGIAVQDHGYMPGMGDRNFRFMKIKEKLDVPRSPEEFAYHGDVPEYFSRMNAVVKSLKGYKPTMMDSKFASVCGATCDDYVKDLESYIVMDIGNGHTLAAACEEGKIIGVFEHHTRALTPDKLKSLVLKLEKGTITHEEVHDDGGHGAWVTGPMSKCECIVATGPLRELLKKTDLKVHNAAPAGDVMMTGPVGLIKSIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W1</accession>
    <name>F0T6W1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="7">3.6.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.3" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644832.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1239"/>
    <dbReference type="EnsemblBacteria" id="ADZ09481">
        <property type="protein sequence ID" value="ADZ09481"/>
        <property type="gene ID" value="Metbo_1239"/>
    </dbReference>
    <dbReference type="GeneID" id="10277690"/>
    <dbReference type="KEGG" id="mel:Metbo_1239"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_4_2"/>
    <dbReference type="OMA" id="FAQPWET"/>
    <dbReference type="OrthoDB" id="21472at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="635"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="682" mass="73906" checksum="BFD61885E2B28089" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X1</accession>
    <name>F0T6X1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase</fullName>
            <ecNumber evidence="2">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.98.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644842.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1249"/>
    <dbReference type="EnsemblBacteria" id="ADZ09491">
        <property type="protein sequence ID" value="ADZ09491"/>
        <property type="gene ID" value="Metbo_1249"/>
    </dbReference>
    <dbReference type="GeneID" id="10277700"/>
    <dbReference type="KEGG" id="mel:Metbo_1249"/>
    <dbReference type="eggNOG" id="arCOG00342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_0_0_2"/>
    <dbReference type="OMA" id="CTGLGYY"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="162"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35650" checksum="BC48A97CB2BF3C0C" modified="2011-05-03" version="1">MKLTPEKNILLFKSCLINVEYPGVESSTKYILDRIGIEYTVDDRQSCCTGLGYYFDLFDQLSTTAIAARNFKIGSSTGHNNIVTMCATCYAILKKSAEILNENSEARNEINDIMVKSGLKNMEYYQGDVDPAENIFHVAEIFFNKRNEISKFVKVDLSDLRVATHHACHYCKVFYKDAIEGVRDPNLLDELVAAVGVKTIGWYDHKKVTCGSGFRQRFINKEISLDVTGEKLQSLKDNNVDVLIHMCPNCQMQFDRYQPCIAEKLETEFEIFHLNIAQFIALAMGADPYTVVGIQTHTVSVTPLIERFKSKFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T707</accession>
    <name>F0T707_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644878.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1285"/>
    <dbReference type="EnsemblBacteria" id="ADZ09527">
        <property type="protein sequence ID" value="ADZ09527"/>
        <property type="gene ID" value="Metbo_1285"/>
    </dbReference>
    <dbReference type="GeneID" id="10277736"/>
    <dbReference type="KEGG" id="mel:Metbo_1285"/>
    <dbReference type="eggNOG" id="arCOG04831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639963_0_0_2"/>
    <dbReference type="OMA" id="YTRGMDT"/>
    <dbReference type="OrthoDB" id="74022at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011313">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09879">
        <property type="entry name" value="DUF2106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019373">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="17850" checksum="245297090DC27575" modified="2011-05-03" version="1" precursor="true">MKSIGRLWNSLANPEKIPRLFALILGIVLIAGLFIPLALDNQQLYPRPAPQEQINAQDPLAPYDRGGIPFEERGIVKAQYPQYAPAIGEITAYLSPIAISVKNTTFYYGTSIYSSPGGLIDEILYYTRGFDTVLESSILMMAFVIASWIALNFTMRRDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T720</accession>
    <name>F0T720_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamylcysteine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644891.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1298"/>
    <dbReference type="EnsemblBacteria" id="ADZ09540">
        <property type="protein sequence ID" value="ADZ09540"/>
        <property type="gene ID" value="Metbo_1298"/>
    </dbReference>
    <dbReference type="GeneID" id="10277749"/>
    <dbReference type="KEGG" id="mel:Metbo_1298"/>
    <dbReference type="eggNOG" id="ENOG502N56R">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_636027_0_0_2"/>
    <dbReference type="OMA" id="WENSMHG"/>
    <dbReference type="OrthoDB" id="40875at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035434">
        <property type="entry name" value="GCL_bact_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011556">
        <property type="entry name" value="Glut_cys_lig_pln_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378">
        <property type="entry name" value="PTHR34378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34378:SF1">
        <property type="entry name" value="PTHR34378:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="460" mass="54418" checksum="51C59E21DA8F944D" modified="2011-05-03" version="1">MNQEDLRNLIYERYIEPTKHKRDVYAGIELELPIINLDKTPVDLKLTHSLTEEFKKQFDFSVKGRDEKGNICALEDTVTDDIYTYDCSYNNLEISFGKEINLNRVEERFKKYYTFIQEFLNSENHFVTGFGINPYRNYNRHLPVPNGRYRMLYHHLCTYQNFRHQKNFHDMPEYGMFTSASQTQIDVHYPDLIKTINLFSRLEPIKAVLFSNSVLREDNMELACSRDMLWENSMQGYNTKNVGMYDPLPETIDELVDYMLQTSIYCTEKGDKYINFPPKVITDFYSEETIHGEYYQKGNYHPISFKPNGTDFHYLRSFKFEDLTFRGTIEFRSVCAQPVNETMTVPSFHMGLINQLDELNQILDYQTSILNQPHSLTELRNQFVRFELPDYVDKNQLKKLITEVLDLATKGLKHRGQNEEKMLKPLYKRVKNLENPGQHLIKHLNSGGELKDIIYQYAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T755</accession>
    <name>F0T755_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646040.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2476"/>
    <dbReference type="EnsemblBacteria" id="ADZ10689">
        <property type="protein sequence ID" value="ADZ10689"/>
        <property type="gene ID" value="Metbo_2476"/>
    </dbReference>
    <dbReference type="GeneID" id="10278950"/>
    <dbReference type="KEGG" id="mel:Metbo_2476"/>
    <dbReference type="eggNOG" id="arCOG01116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098525_0_0_2"/>
    <dbReference type="OMA" id="RHQLYVH"/>
    <dbReference type="OrthoDB" id="73767at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="234" mass="25477" checksum="F7FAE6D603B97217" modified="2011-05-03" version="1">MIYVCLDDTDNLESRGTGKLSRTIANELSKNYPVYGVTRHQLYKHPEIPYTSHNTCSVIHIETDDDIDVKYNDELFEAVKFEMMADFIDGSDPGLAVAHEDQIKPALTAFGNDAKYKILNQQLARGLAKNLGIRLEGLGGTEDGVIGAMAGIGLAHTKNDGRFLLVKNNKLKGSNTVETVLNSGVDAVYTIDGRLVTKGVIVNDSKLTKPCPVNGEVILFVVEENGLLKAVNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T778</accession>
    <name>F0T778_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RHH_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2499"/>
    <dbReference type="EnsemblBacteria" id="ADZ10712">
        <property type="protein sequence ID" value="ADZ10712"/>
        <property type="gene ID" value="Metbo_2499"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2499"/>
    <dbReference type="eggNOG" id="arCOG14022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3113067_0_0_2"/>
    <dbReference type="OMA" id="MDKQITM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038733">
        <property type="entry name" value="Predicted_DNA_bind_prot_RHH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12651">
        <property type="entry name" value="RHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_3" evidence="1">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="50" mass="5950" checksum="329CFC42BE8BAE49" modified="2011-05-03" version="1">MDKQITMKIPEEMYSDLKKLANQKDDIPMANLIRRAIDDYLRKNRLKGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T798</accession>
    <name>F0T798_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, inner membrane subunit CbiQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643678.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0074"/>
    <dbReference type="EnsemblBacteria" id="ADZ08327">
        <property type="protein sequence ID" value="ADZ08327"/>
        <property type="gene ID" value="Metbo_0074"/>
    </dbReference>
    <dbReference type="GeneID" id="10276497"/>
    <dbReference type="KEGG" id="mel:Metbo_0074"/>
    <dbReference type="eggNOG" id="arCOG02250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_1_2_2"/>
    <dbReference type="OMA" id="IASFMIR"/>
    <dbReference type="OrthoDB" id="75740at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="277" mass="31911" checksum="35A71F8C1532CF27" modified="2011-05-03" version="1">MNGVSSILQLETETNKKSPLHAIDGRVKLVLLIFIIVYSVFSTQILVLIFLEIYLLILMYISNISFKTSLTRILLLLPFGGFIIAFQPFIHPGIIIWSGAFGIHITDAGLLWAVLLMSRLVVALTSIVILSSISPMQEVVESFRKLGMPREFALIFSLMIRFLFMFFDELNRIMHAQKTRCFDAFNKKLPYTWRMKQLGYTVAMMFLRAFERGETIYMSMASRGYSDKSHLYSDTRRKFGTPEYIFILATLSTVVCLQYLAMYLFTQFGILGMYIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C5</accession>
    <name>F0T7C5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="16">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
                <ecNumber evidence="16">2.3.1.234</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="16">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">Serine/threonine-protein kinase Bud32</fullName>
                <ecNumber evidence="16">2.7.11.1</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Metbo_0101</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AL-21</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 16">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 16">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="16"/>
    <dbReference type="EC" id="2.7.11.1" evidence="16"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643705.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0101"/>
    <dbReference type="EnsemblBacteria" id="ADZ08354">
        <property type="protein sequence ID" value="ADZ08354"/>
        <property type="gene ID" value="Metbo_0101"/>
    </dbReference>
    <dbReference type="GeneID" id="10276526"/>
    <dbReference type="KEGG" id="mel:Metbo_0101"/>
    <dbReference type="eggNOG" id="arCOG01183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OMA" id="GETMDTG"/>
    <dbReference type="OrthoDB" id="9881at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase_NBD"/>
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        <property type="entry name" value="tRNA_threonyl_synthase/kinase"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 16 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="334"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Kae1" evidence="16">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Threonylcarbamoyl-AMP binding" evidence="16">
        <location>
            <begin position="129"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for kinase activity" evidence="16">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
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        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
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        </ligand>
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            <position position="129"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
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        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
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        </ligand>
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        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
        </ligand>
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        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>L-threonylcarbamoyladenylate</name>
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        </ligand>
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        </location>
        <ligand>
            <name>Fe cation</name>
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        </ligand>
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        <location>
            <begin position="340"/>
            <end position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="544" mass="59663" checksum="3BA719E3420D2884" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D0</accession>
    <name>F0T7D0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nonspecific acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0106</name>
    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_0106"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="35302" checksum="9DEF6EEF6326720B" modified="2011-05-03" version="1">MSKNKLTSWNDGLIKQSILEFLESSVESGPNFIKPEDRIASFDYDGTLSIEKPAPAQILYLISTLISKAEEYPHLKDKEPYKSIFEIENNVKVISDEEKPRIVKLMAETYANWSYGQTPEEYEEDVTNFFQKVKQEKFGVCYTDLIYKPMLELFELLKEYEYRIFLCTGSGREFIRTISERVFNIPKENIIGTTEIYEYYNGGLRKVKEPFKQIVVGPGKVEHIFTRTGRLPAFAAGNSNGDIEMLEASEFKLLINHDDDKREYAYQTGAEKMLEVASENNYTVVSMKMTGKRFSTTNLAEIHTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D3</accession>
    <name>F0T7D3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Aspartate transcarbamylase</fullName>
            <shortName evidence="5">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrB</name>
        <name evidence="9" type="ordered locus">Metbo_0109</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643713.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0109"/>
    <dbReference type="EnsemblBacteria" id="ADZ08362">
        <property type="protein sequence ID" value="ADZ08362"/>
        <property type="gene ID" value="Metbo_0109"/>
    </dbReference>
    <dbReference type="GeneID" id="10276534"/>
    <dbReference type="KEGG" id="mel:Metbo_0109"/>
    <dbReference type="eggNOG" id="arCOG00911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="OrthoDB" id="51351at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="155"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="307" mass="34385" checksum="BBAA569684F41BBC" modified="2011-05-03" version="1">MVFNQKNIISISDFSKDDIEYILRLSDKMEPIARSRKTSNVLSGKLLGMLFYEPSTRTRLSFEASMKRLGGSTIGFAEANVSSATKGENLTDTVKIVGEYSDAIVIRHNMEGAARFASGIVDVPVINAGDGAGQHPTQTILDLYTMKKIFGKIDDLHVALIGDLKYGRTVHSLAYALAMFDVKMSFVSPPELKMPEGIVHDLNKAGVSVQQTDDVHSVLEDSDIFYVTRIQKERFPDPEEFSRIKGAYMIDKKLVEDTDLIVMHPLPRIDEISYDLDDTKHAKYFEQAFYGVPVRMALLKSIIESYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H8</accession>
    <name>F0T7H8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1304"/>
    <dbReference type="EnsemblBacteria" id="ADZ09546">
        <property type="protein sequence ID" value="ADZ09546"/>
        <property type="gene ID" value="Metbo_1304"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1304"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_4_1_2"/>
    <dbReference type="OMA" id="NGFDNEY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="334" mass="38248" checksum="B6F7B9D1D39900DF" modified="2011-05-03" version="1">MEPKVSIILLNWNGWEDTIECLESLYQINYSNYNVILVDNHSEDKSIKKIREYLNGDLPIRSEFYEYRSSNKPIKLLENGEKPETGDGSLILLKNSENLGFAAGNNVGINHALENLDPDYVLLLNNDTVVDKNFLDELVKTGESNAEIGFVGAKTLFYDREGIVQEAGGGMIDYTHAKVEEIGYNEVDDGSLDNYIEPDYIGGACVLVKAGVIKKIGGLDPNYFMYWEDVDWCITGFEEGYKSAYQYQAVIRHKYGSSSQNTFKMYYLNRNRLYFMKKHTSGGKYLKFLAYFVPFILFETTYQLIRKSDSKMAKAHFYAMLDGFKLAKNPIKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I8</accession>
    <name>F0T7I8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644907.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1314"/>
    <dbReference type="EnsemblBacteria" id="ADZ09556">
        <property type="protein sequence ID" value="ADZ09556"/>
        <property type="gene ID" value="Metbo_1314"/>
    </dbReference>
    <dbReference type="GeneID" id="10277765"/>
    <dbReference type="KEGG" id="mel:Metbo_1314"/>
    <dbReference type="eggNOG" id="arCOG01408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_5_2_2"/>
    <dbReference type="OMA" id="WREDWDW"/>
    <dbReference type="OrthoDB" id="59830at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="203"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="392" mass="43989" checksum="9CBE0ED752C7BAA0" modified="2011-05-03" version="1">MDLKSAAGSVTKSDTKILMVHHTAMWYRKPFFRMLSGIYPIKFLFTNVESYNKTYDTELSQEIEGLDGVDYTVVNSYHGLALGAMKATMGDYDVFVGGSWDTLTDLIETLVYFAVVKIRRKPFILWREDWDWNVKSLKRSMVKKFAGFIGRNVDAVLVPGTKHREFFTKLGVKPENMFIMPNVSNIKAKEVDELNMVQIQEELGLENKKVALYVGRLIDLKGVDYLIKGFKGVTEKLDDAVLLIVGEGPEKPALESLTSELSMDGEVIFTGNIDNSKLGAYYMVADLFVLPSITTYFADACPLVVNEAMYFSKPVITSDAVGTTFMIKDGENGYVVPEKNSEALKTAILTVLSNPELIKNMGIASKNLIETNFKYDNMIQGFNSAIEHVQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K7</accession>
    <name>F0T7K7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1334"/>
    <dbReference type="EnsemblBacteria" id="ADZ09575">
        <property type="protein sequence ID" value="ADZ09575"/>
        <property type="gene ID" value="Metbo_1334"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1334"/>
    <dbReference type="eggNOG" id="arCOG06473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536692_0_0_2"/>
    <dbReference type="OMA" id="HYVFTTS"/>
    <dbReference type="OrthoDB" id="100852at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="18774" checksum="63F88DB49244C6FC" modified="2011-05-03" version="1">MDDHGQMSAEFILLVGIGIVSTLLFAHASFEAVELNTVMSAARNGVTEGIALDSLAVYPSEKFKKYSESYPRLKTCSKTVYIDTKWLNMGYDSTYQKTKILIKIRASTQDQMTKIERDCLGDRINYYVRKNICETFKTDNMTNIYYNPAFSDHYIITTSNVEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L1</accession>
    <name>F0T7L1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1338"/>
    <dbReference type="EnsemblBacteria" id="ADZ09579">
        <property type="protein sequence ID" value="ADZ09579"/>
        <property type="gene ID" value="Metbo_1338"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1338"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OMA" id="QRNTMAF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="26749" checksum="F9FD4CB04D0262D7" modified="2011-05-03" version="1">MIKTTKSGIPGFDELTSTDEFPGGIPLSRTTLIYGPPKTGKTIFANQFTYDGLENNLPCLYITADQGIKQLTTSMMEFNWFLQRYIESGDIYIIDGISQLGGVKLDDSVNYKTTTIGNPAELMIKVGIGSRYVYRKSPEFRSVLDSATTMFAFNQEQMVLRVLNAYIRRNNEAGGAGLMTYTEGSTTDKTEQQLLSLFDNLIRMDGNTITIRSEYNQSEKPDNFKSVYSISENGIVVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P2</accession>
    <name>F0T7P2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thermosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1370"/>
    <dbReference type="EnsemblBacteria" id="ADZ09610">
        <property type="protein sequence ID" value="ADZ09610"/>
        <property type="gene ID" value="Metbo_1370"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1370"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="PTHR11353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="540" mass="58070" checksum="81FC4C3645F27B86" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q0</accession>
    <name>F0T7Q0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase (Ubiquinone) 24 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2519</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646083.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2519"/>
    <dbReference type="EnsemblBacteria" id="ADZ10732">
        <property type="protein sequence ID" value="ADZ10732"/>
        <property type="gene ID" value="Metbo_2519"/>
    </dbReference>
    <dbReference type="GeneID" id="10278993"/>
    <dbReference type="KEGG" id="mel:Metbo_2519"/>
    <dbReference type="eggNOG" id="arCOG04890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_1_2"/>
    <dbReference type="OMA" id="HIIRYCD"/>
    <dbReference type="OrthoDB" id="107119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="158" mass="17524" checksum="39281FE68F13CD89" modified="2011-05-03" version="1">MTKTLNEILSTYEGTKSELIPLLQDVQANLGYLSEESIKDVSKFTGVSESEIYGVATFYTQFRFTPVGKKHIMVCKGTACHVKGAPQIIEGIERHLGIKEGEVTFDMEYSLESVGCLGCCALAPCAMINDDVESNMTLKDVKKIFRRIERQSKSDGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R1</accession>
    <name>F0T7R1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646094.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2530"/>
    <dbReference type="EnsemblBacteria" id="ADZ10743">
        <property type="protein sequence ID" value="ADZ10743"/>
        <property type="gene ID" value="Metbo_2530"/>
    </dbReference>
    <dbReference type="GeneID" id="10279004"/>
    <dbReference type="KEGG" id="mel:Metbo_2530"/>
    <dbReference type="eggNOG" id="arCOG09288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145991_0_0_2"/>
    <dbReference type="OrthoDB" id="106865at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="14044" checksum="ADF82A066DE35CD0" modified="2011-05-03" version="1">MKPENEDFNLKKRLENIHKDIDPMKTEKFRLSEINYDYVQNELLTPNFMEKHTEFKNFSEMVRSSDFKIENEIEFRDAIETEKWNLLVKENSDFHDWNEMLRVAVIDRATRKLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S3</accession>
    <name>F0T7S3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0158"/>
    <dbReference type="EnsemblBacteria" id="ADZ08410">
        <property type="protein sequence ID" value="ADZ08410"/>
        <property type="gene ID" value="Metbo_0158"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0158"/>
    <dbReference type="eggNOG" id="arCOG01156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_3_2_2"/>
    <dbReference type="OMA" id="NDYEVSQ"/>
    <dbReference type="OrthoDB" id="49806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="58"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="294" mass="32721" checksum="D5E0E06D7E1228B3" modified="2011-05-03" version="1">MKETNPHIRALYLRQKMQTAMSGVRDKLGIHEFDARDFEIVPVTVHVNGLDPEFNNYKIAHISDIHLGQWISAKRIEGVVNLVNKQKPDIVAITGDSVSYVVNEPILDMLRYLKNLKPKDATLAVLGNHDHWIGADEIRKVMKETGIIELENDVYTIKRGDAQLNIAGIDSVTLDKHDLNAVLNKLPESGPAILLAHEPDFADVSAATGRFSLQLSGHSHGGQMIIPGVGTPFRGSEFKKYPLGKYDVGDMVQYTNRGLGTNVFWIRINCPPEITVLNLQSSNSEIKDEPNKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y5</accession>
    <name>F0T7Y5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase, subunit gamma</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643823.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0220"/>
    <dbReference type="EnsemblBacteria" id="ADZ08472">
        <property type="protein sequence ID" value="ADZ08472"/>
        <property type="gene ID" value="Metbo_0220"/>
    </dbReference>
    <dbReference type="GeneID" id="10276648"/>
    <dbReference type="KEGG" id="mel:Metbo_0220"/>
    <dbReference type="eggNOG" id="arCOG02473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="HESFVPI"/>
    <dbReference type="OrthoDB" id="54703at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="28416" checksum="BA874574144F4783" modified="2011-05-03" version="1">MLARIKQFLGIGAKPKEVPKEEVEKVAEEKAKPRIGYVHLSGCTGDIMSLSENYDILAELLTNMVDIVYGQTLADVWEMPEMDLVLVEGSVCLQDEHSVKELKEAREKAGLVVAFGSCAATGCFTRYSRGGQQARPDHESFVPIADLVKVDCAIPGCPPSPEIIAKTVVAVINGDMDYLQPMIDLAGYTEACGCDLQYKVVNQALCIGCGTCAMACQTRALTMTDGRPELTSARCVKCGICYVQCPRSWWPADRIKQDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z9</accession>
    <name>F0T7Z9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil/vitamin C permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1385</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ09625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1385"/>
    <dbReference type="EnsemblBacteria" id="ADZ09625">
        <property type="protein sequence ID" value="ADZ09625"/>
        <property type="gene ID" value="Metbo_1385"/>
    </dbReference>
    <dbReference type="GeneID" id="10277836"/>
    <dbReference type="KEGG" id="mel:Metbo_1385"/>
    <dbReference type="eggNOG" id="arCOG02807">
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    <dbReference type="HOGENOM" id="CLU_024508_0_0_2"/>
    <dbReference type="OMA" id="RKGSYFF"/>
    <dbReference type="OrthoDB" id="29114at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="485" mass="52470" checksum="E2C7E8B43BB9BF81" modified="2011-05-03" version="1">MYQRAYERKEKFKNSFRSYVDNYFGITKYNSSVKTEILAGLTTFMTMAYIIIVNPLILSTAEMDYKAVFVATCLSAATATLFMGLIAKKPFGMATGLGLNSVVAFGLILTLNIPWQIAMGLIFIEGFLVFILVLTNLRELVMHAIPQSLKMSIGVGIGMFISFIGFKLAHIVTASPENLIKFGDVTNPVFIIAALGVSIIMVMMALKIKGSILYGIIITALIAVGACVVADYFHVAFNLFGNPNLPSSIAPLASGMSNLPQTFDGTLLELPNAQSLSTIGHLDIWGALSLSFVPIVFALMMVDFFDSLGTVMAVGTQAGLVDKKGKLLDIKKVLAVDAMGAMIGGFMGSSSNTAYVESSAGVREGGRTGLTSVVIACLFLVGMLFVPLAYYIPAAATAPALIIVGFFMITMIQKIEWDNFEEALPAFLTITGMTFTFSISKGIGFGFISYCIIKLALGKWREVHPLMWVVSVIFLIYFIFVSNMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T848</accession>
    <name>F0T848_METLA</name>
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        <submittedName>
            <fullName evidence="2">NADPH dehydrogenase</fullName>
            <ecNumber evidence="2">1.6.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645025.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1436"/>
    <dbReference type="EnsemblBacteria" id="ADZ09674">
        <property type="protein sequence ID" value="ADZ09674"/>
        <property type="gene ID" value="Metbo_1436"/>
    </dbReference>
    <dbReference type="GeneID" id="10277887"/>
    <dbReference type="KEGG" id="mel:Metbo_1436"/>
    <dbReference type="eggNOG" id="arCOG00615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_3_2"/>
    <dbReference type="OMA" id="NQRRDQY"/>
    <dbReference type="OrthoDB" id="23479at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018548">
        <property type="term" value="F:pentaerythritol trinitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="346" mass="38608" checksum="1DE3E9B101023426" modified="2011-05-03" version="1">MKSLFETTEISGMELKNRFICSAIWEGLADDEGHVTQELIHHYHKLAEGGVGTIITGFSNVMDFDKPTHNMNGIYNDSFIQEYEELIEKVHGHRVNIIMQIVHGGTKWGPSAVKHKATGTVPKEMTTDEINAVTQAFGEAALRVKNAGFDGVQIHAAHGFLLSMFLNPYYNVRTDEYGGSIDNRARIVYETYKSVRDAVGPDFPVFIKVNCKDFMDEGLTFEDSLHVCKKLADMGINMIEVSGGSYSSRDGEGPMRNTENNESYFMDYASKIAEEVDVSVALVGGNRRIEKMTEILNTTQIDYFSMARPFIHEPGLINKWMEHDLDSSECVSCHACNGISGGCVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T856</accession>
    <name>F0T856_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GAF domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1444"/>
    <dbReference type="EnsemblBacteria" id="ADZ09682">
        <property type="protein sequence ID" value="ADZ09682"/>
        <property type="gene ID" value="Metbo_1444"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1444"/>
    <dbReference type="eggNOG" id="arCOG02338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541449_0_0_2"/>
    <dbReference type="OMA" id="EECMLDK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="209"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="367"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="512" mass="57656" checksum="468B65B6A612984E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D0</accession>
    <name>F0T8D0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643893.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0290"/>
    <dbReference type="EnsemblBacteria" id="ADZ08542">
        <property type="protein sequence ID" value="ADZ08542"/>
        <property type="gene ID" value="Metbo_0290"/>
    </dbReference>
    <dbReference type="GeneID" id="10276722"/>
    <dbReference type="KEGG" id="mel:Metbo_0290"/>
    <dbReference type="eggNOG" id="arCOG04212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114051_3_1_2"/>
    <dbReference type="OMA" id="HLHMSVA"/>
    <dbReference type="OrthoDB" id="124564at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03479">
        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="13509" checksum="CE31551DDBB9A549" modified="2011-05-03" version="1">MIVSRLLPGDDLKTSLENISDSIEFNSGVIVSVVGSLTRTHLRMSNGTKKLFEGFFEIVSVEGTISADGVHIHMAVAAEDGSVFGGHLLEGCIIHTTAEVCIIEAEKEFKRVFDQNTGYRELQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E8</accession>
    <name>F0T8E8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645050.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1462"/>
    <dbReference type="EnsemblBacteria" id="ADZ09699">
        <property type="protein sequence ID" value="ADZ09699"/>
        <property type="gene ID" value="Metbo_1462"/>
    </dbReference>
    <dbReference type="GeneID" id="10277913"/>
    <dbReference type="KEGG" id="mel:Metbo_1462"/>
    <dbReference type="HOGENOM" id="CLU_2243869_0_0_2"/>
    <dbReference type="OrthoDB" id="120107at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="12256" checksum="3714F06B48C6C04C" modified="2011-05-03" version="1">MKAKKLKIYVKTSKFAIPIPALRFSTIRWISKLIFKYCPPKVRSNKSKSELGEYDTMEYIMKNLTLEDVNLLIDELEKEEPFCLVDVDTYNKKEGRTIVKIYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G5</accession>
    <name>F0T8G5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645067.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1482"/>
    <dbReference type="EnsemblBacteria" id="ADZ09716">
        <property type="protein sequence ID" value="ADZ09716"/>
        <property type="gene ID" value="Metbo_1482"/>
    </dbReference>
    <dbReference type="GeneID" id="10277933"/>
    <dbReference type="KEGG" id="mel:Metbo_1482"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_3_2_2"/>
    <dbReference type="OMA" id="TGHDCMI"/>
    <dbReference type="OrthoDB" id="58184at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="70"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="24733" checksum="39405F6F542E7CD6" modified="2011-05-03" version="1">MNTHKKSSYVLVHGGNMSTETWNKLAEIKVCTPDGKMGGKVWNTIKPLIEENDHLVFAPTLLDEHSSTLTEHIDQIKRLILKNNLEYVVLVGHSYGGMIITGVAAEMPDRIRSMVYIDAAVPDPGQSLFDIIASGGCDPLSFNGLEPAAPYIEKLDFDALKIKNIPKTYILCTESIFADATKVATEKIASNREGWTYYELKSSHIPMATMPKQLLQLILKPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N1</accession>
    <name>F0T8N1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="2 6">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purM</name>
        <name evidence="9" type="ordered locus">Metbo_0321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643924.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0321"/>
    <dbReference type="EnsemblBacteria" id="ADZ08573">
        <property type="protein sequence ID" value="ADZ08573"/>
        <property type="gene ID" value="Metbo_0321"/>
    </dbReference>
    <dbReference type="GeneID" id="10276758"/>
    <dbReference type="KEGG" id="mel:Metbo_0321"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="62508at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="7">
        <location>
            <begin position="49"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="8">
        <location>
            <begin position="167"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="342" mass="36976" checksum="F5F32EAE3CA98F06" modified="2011-05-03" version="1">MVTYSESGVDISLEEVTVSALISKLKETLNYRDVITESGHFAALVNLGDRAIAMSTDGVGSKILVAEMMEKYDTVGIDCIAMVVNDIICVGAEPIAMVDYLAVEKPDPEVAEQIGEGLRIGCKLANVAMIGGETASLPEIVKNFDLAGTGIGIVDTDSIVTGSSMVEGDVLIGIESSGIHSNGLSLARNIFFEENDFKVTDKLPNNPETTIGEELLKPTNIYVNPVIELLKTDVEVHGLAHITGGGFTNLKRLKKGVGYNITSLPEPQSIFLEMYELGVPVEELYRVFNMGIGFVVIVKPGDETRTMELIEKYNRAYKIGHVTADPDDKVTIKTFKEGTIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N4</accession>
    <name>F0T8N4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="3">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purP</name>
        <name evidence="6" type="ordered locus">Metbo_0324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + ATP + formate = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643927.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0324"/>
    <dbReference type="EnsemblBacteria" id="ADZ08576">
        <property type="protein sequence ID" value="ADZ08576"/>
        <property type="gene ID" value="Metbo_0324"/>
    </dbReference>
    <dbReference type="GeneID" id="10276761"/>
    <dbReference type="KEGG" id="mel:Metbo_0324"/>
    <dbReference type="eggNOG" id="arCOG04346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="OMA" id="CIHYFYS"/>
    <dbReference type="OrthoDB" id="21169at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="PTHR38147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="118"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="363" mass="41238" checksum="B543C9B26F391C23" modified="2011-05-03" version="1">MVKVNRNEILEILKGYDKEDITIATLGSHTSLHILKGAKEEGFRTAVVCEKGREVPYKRFKVADEYIIVDKFSDIVNEDVQEKLRAMNSIVVPHGSFVAYAGLDRIENDFKVPMFGNRDILRWEAERDLERELMIESDIRIPLKFEDPSKIDRTVMVKFPGARGGRGYFVASTPEEFHQKIDSMLERNWIGEEDVENAHIEEYVSGCNYCVHYFYSPLNNEVEVLGMDSRYESNIDGLTRIPAKDQLDVSLDPSYVITGNHPVVMRESLLPQVFEIGDNLVEGAKKLVSPGMSGPFCLQTLCTDDLEIIVFEMSARIDGGSNTFMDSSAYSYLLFGEFMSMGRRIAREIKEGAKQDKLDILIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S7</accession>
    <name>F0T8S7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0367</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0367"/>
    <dbReference type="EnsemblBacteria" id="ADZ08619">
        <property type="protein sequence ID" value="ADZ08619"/>
        <property type="gene ID" value="Metbo_0367"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0367"/>
    <dbReference type="eggNOG" id="arCOG04236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_2"/>
    <dbReference type="OMA" id="IMFDEPD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28581" checksum="DBF2B5496E05CC72" modified="2011-05-03" version="1">MVVTLLLEITDLAVEVNGKEILKDIDLYIDKGETHVLLGPNGSGKSTLFMSLLGFPKYNITRGEILFKDQDITHMTTTERIELGLGVSFQNPPAIRGVKLKDLLNIETSKKGKSPMGQELSPETLALADKLKLNDQFLERDVNLGFSGGEVKRSEILQLLAQQPDFIMFDEPDSGVDIENVELLAEEINVLLDKDKKPGLREKSGLLITHLGYILNFVGANTAHVLMDGKIACSGDPNEILEDIRKEGFKGCVECCKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W1</accession>
    <name>F0T8W1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645140.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1562"/>
    <dbReference type="EnsemblBacteria" id="ADZ09789">
        <property type="protein sequence ID" value="ADZ09789"/>
        <property type="gene ID" value="Metbo_1562"/>
    </dbReference>
    <dbReference type="GeneID" id="10278018"/>
    <dbReference type="KEGG" id="mel:Metbo_1562"/>
    <dbReference type="eggNOG" id="arCOG00801">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_700014_0_0_2"/>
    <dbReference type="OMA" id="VMEEAYL"/>
    <dbReference type="OrthoDB" id="49930at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="218"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="396" mass="46180" checksum="DB4B7B1033242D48" modified="2011-05-03" version="1">MSLSVRSKPYIIPEYSLTGDLLAYLTCGLQYRYQNKGTLPPAMPIQLWFGNFIHGVMEEAYLRWKEDDDWKDFPWDWKTQIRDIELSIDKMMRARGMQPPANLFCPFHEETEELGLCPDHDHPHKLAASIRAEASINTWGPHLFPLIDDSEVKLKGIRDMPNYEKDQSRSNYYGIKGIIDVLSSVKIDEASDKNLIIKYLHNDPAFQYKLEKLDKPEYEIIVDYKGMKRPQLDSPSWNHHNWQVLTYSWLRSMQPESRPVLIGILFYLNELSLFKEDLKELKKDVETGKTDIKPFADDLKNILNWNPHHKPPKISDSLKSLRSIRIIPVEYDNLETSLKEFDYVVADIEKSILMEVNGQDIQSSWSPNPDERTCDACDFTTFCKDNKSSKRFPSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X2</accession>
    <name>F0T8X2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645151.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1573"/>
    <dbReference type="EnsemblBacteria" id="ADZ09800">
        <property type="protein sequence ID" value="ADZ09800"/>
        <property type="gene ID" value="Metbo_1573"/>
    </dbReference>
    <dbReference type="GeneID" id="10278029"/>
    <dbReference type="KEGG" id="mel:Metbo_1573"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340742_0_0_2"/>
    <dbReference type="OMA" id="ETAVYCK"/>
    <dbReference type="OrthoDB" id="86065at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="21123" checksum="6F5BAE2F50E5D46F" modified="2011-05-03" version="1">MKCDKCGRENNETAVYCKSCGAALKDKENLIVRINARLNFLAVTLGLIVFIIVMFITSFLFIGLVTFKAVPAVVYILLVLVISVFVGSIIPGVWGSKTVDDGYINGSFLSLVVLVLAGIIAAFIFLAFMGVASILASAFGPASSLLGSGTSGAVSSLNSTSSASGTNVLNLILNLVELIASLVLILVSGAIGGALGVLIKDQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X3</accession>
    <name>F0T8X3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645152.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1574"/>
    <dbReference type="EnsemblBacteria" id="ADZ09801">
        <property type="protein sequence ID" value="ADZ09801"/>
        <property type="gene ID" value="Metbo_1574"/>
    </dbReference>
    <dbReference type="GeneID" id="10278030"/>
    <dbReference type="KEGG" id="mel:Metbo_1574"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145230_0_0_2"/>
    <dbReference type="OrthoDB" id="127685at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="242" mass="26124" checksum="A98737D20D5279FC" modified="2011-05-03" version="1">MVKCQRCGQENDGDADFCENCGANLNPLKNGGSRQPPHDRGMKQSTKILITVCILLVAVLGVSAAALSQLNKVATVPGSTVNSVNQSTSSTNNVKSSANAGPKTFSNGIIYFQYPSNWDVLPNTSNIMAIVGLPHYPSFSVYDESKYGYGSLSEYVSSSESQMTSNGFSIQSERNILVDGLHAHETTYQGKSGNGKLIIQRMVLVEKSPGSEYYALVGADNANQYDQDSSSFDQIMNSFKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y2</accession>
    <name>F0T8Y2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645161.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09810">
        <property type="protein sequence ID" value="ADZ09810"/>
        <property type="gene ID" value="Metbo_1583"/>
    </dbReference>
    <dbReference type="GeneID" id="10278039"/>
    <dbReference type="KEGG" id="mel:Metbo_1583"/>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891445_0_0_2"/>
    <dbReference type="OrthoDB" id="139694at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="14480" checksum="9152B2F52D08913D" modified="2011-05-03" version="1" precursor="true">MKMINNKVTYATFLLVLTCALTLGYCGSHVSALVSGDSIQATDLTVNNIHGSTGHYTFLKAYLSDEHGKAVPNKCITFKVDGDPHDYIAVTSPNGHALLYYYIPQNPGTYSIKTEFKGDENFAPSVSTGVLTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y5</accession>
    <name>F0T8Y5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ppx/GppA phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645164.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1586"/>
    <dbReference type="EnsemblBacteria" id="ADZ09813">
        <property type="protein sequence ID" value="ADZ09813"/>
        <property type="gene ID" value="Metbo_1586"/>
    </dbReference>
    <dbReference type="GeneID" id="10278043"/>
    <dbReference type="KEGG" id="mel:Metbo_1586"/>
    <dbReference type="eggNOG" id="arCOG05138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_1_0_2"/>
    <dbReference type="OMA" id="IGCVRMT"/>
    <dbReference type="OrthoDB" id="39069at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="307" mass="34555" checksum="57057E29E96B99B2" modified="2011-05-03" version="1">MTVGIIDIGSNSVKLNAYRSNADAVHIIFSRKESLGLVFYVKKGILTDEGIEKLLDVLKSLKTDLDYLKIDNYRFFATASLRNIKNSAQVIQIVKDEVGIDIDLLSSSEEGELSFLGSLSSIKEDDGVLIDVGGGSVEVVPYKNREITQKYSIPVGSLKMYNQYVSNLIPTELECYKIEKRVFSELIKLGVKNDEKKEFLCTVGGSVRAIEQLMVDLKIKSKDEEFIDIKQFMELKNQLHGNTKETYNKILHVKPSRIHLLVPTLMIVIAVASYFGCEKVQVSKFSVREGYLQKKILKRCPNVTMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y6</accession>
    <name>F0T8Y6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645165.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1587"/>
    <dbReference type="EnsemblBacteria" id="ADZ09814">
        <property type="protein sequence ID" value="ADZ09814"/>
        <property type="gene ID" value="Metbo_1587"/>
    </dbReference>
    <dbReference type="GeneID" id="10278044"/>
    <dbReference type="KEGG" id="mel:Metbo_1587"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="ATMLKPN"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="25374" checksum="05D82B272542C72E" modified="2011-05-03" version="1">MQNLVNGKELWKTYDLGGVKVNALRGLNITVDEGEFVSIMGPSGSGKSTLMNLIGCLDTPTEGELTIDGREISSMKESELTKVRAEKIGFIFQTFNLLPALSVRDNVEFPMRNLSGDKKLNKSARRARAEECLECVGLGHRLDHLPAKLSGGERQRVAIARALVNKPKFILADEPTGNLDTESTENIMKLLHDINDMGTTVIMVTHDVETTENTRILRIRDGVIEPNKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z8</accession>
    <name>F0T8Z8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Filamentation induced by cAMP protein Fic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645177.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09826">
        <property type="protein sequence ID" value="ADZ09826"/>
        <property type="gene ID" value="Metbo_1599"/>
    </dbReference>
    <dbReference type="GeneID" id="10278056"/>
    <dbReference type="KEGG" id="mel:Metbo_1599"/>
    <dbReference type="eggNOG" id="arCOG03110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762693_0_0_2"/>
    <dbReference type="OrthoDB" id="55690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="101"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="322" mass="37308" checksum="C25F81058B23608E" modified="2011-05-03" version="1">MFDPKFNYTHQLVDVLSQISAADAIINNTNLFKSYDLLFKDSAMVKTAYHSISIAGNPLTIKQVNQLFNGEEVQASEVGVWEVLNYFTVIEKLDKFHEAGLNEDTLGEINRYLTTGTLKNPALSGNYKNSQLKVAMHEFFTWLRNENYLHPVLTAAIAHHAILNKLPFSKGNGKTARVMVHLIFKQKQFNCRGYYQLDEEFHENITAYKEAVLKSTETEDMTNWLEYFAGVVLVSILRTKKYVLDIYREKCIPHNMNQLPISPNEIKIFQFLRVHKRIRSQDIQRMFGVSHPGVFKYIQKLLDYGVIKPLGRGRNTYYVFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T920</accession>
    <name>F0T920_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytosine deaminase</fullName>
            <ecNumber evidence="2">3.5.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643993.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0390"/>
    <dbReference type="EnsemblBacteria" id="ADZ08642">
        <property type="protein sequence ID" value="ADZ08642"/>
        <property type="gene ID" value="Metbo_0390"/>
    </dbReference>
    <dbReference type="GeneID" id="10276827"/>
    <dbReference type="KEGG" id="mel:Metbo_0390"/>
    <dbReference type="eggNOG" id="arCOG01488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_7_2_2"/>
    <dbReference type="OMA" id="AILHAEM"/>
    <dbReference type="OrthoDB" id="81620at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102480">
        <property type="term" value="F:5-fluorocytosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004131">
        <property type="term" value="F:cytosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="157" mass="17589" checksum="36FC355CE1B3DE55" modified="2011-05-03" version="1">MIETNNSEDDYKFMMEALKEAKKSLESGGIPIGAILVKDGVIVGRGHNNLLQKNSTILHAEMDCIENSGRLKGKDYKRSTLYTTLSPCEMCSGTIILYKISRVVIGENENLKGPEKCLIESGIELKNLDLPECKEMLGSYILQNPDLWADEIERIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T928</accession>
    <name>F0T928_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644001.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0398"/>
    <dbReference type="EnsemblBacteria" id="ADZ08650">
        <property type="protein sequence ID" value="ADZ08650"/>
        <property type="gene ID" value="Metbo_0398"/>
    </dbReference>
    <dbReference type="GeneID" id="10276835"/>
    <dbReference type="KEGG" id="mel:Metbo_0398"/>
    <dbReference type="eggNOG" id="arCOG01330">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117037_0_0_2"/>
    <dbReference type="OMA" id="WDRYGLP"/>
    <dbReference type="OrthoDB" id="114784at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009577">
        <property type="entry name" value="Sm_multidrug_ex"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06695">
        <property type="entry name" value="Sm_multidrug_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="15921" checksum="2FAB286F275F7C5E" modified="2011-05-03" version="1">MNIFESILLVFLASVVELWLSIPLGLVLKLNPILIIVAAAAGSIISAFLVVTLGEGVRKWFIKWRYGSGSIEHGRIYKIWNKYGIIGLGLLSPLLFGAPLGAAIGVGLGAPKNRLLLWMSVGIVIWSVVLTLVAFYGLLNLESFTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T934</accession>
    <name>F0T934_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-ribbon RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644007.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0404"/>
    <dbReference type="EnsemblBacteria" id="ADZ08656">
        <property type="protein sequence ID" value="ADZ08656"/>
        <property type="gene ID" value="Metbo_0404"/>
    </dbReference>
    <dbReference type="GeneID" id="10276841"/>
    <dbReference type="KEGG" id="mel:Metbo_0404"/>
    <dbReference type="eggNOG" id="arCOG01989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196471_1_0_2"/>
    <dbReference type="OMA" id="KCGFEGP"/>
    <dbReference type="OrthoDB" id="123853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO_2753_ZBP" evidence="1">
        <location>
            <begin position="6"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="6213" checksum="BAE613D81E2323F3" modified="2011-05-03" version="1">MKKIECTSCKQEISPVENYVKFPCPECEKTLYRCQKCRTFGHLYKCECGFKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T982</accession>
    <name>F0T982_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Filamentation induced by cAMP protein Fic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645184.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1606"/>
    <dbReference type="EnsemblBacteria" id="ADZ09833">
        <property type="protein sequence ID" value="ADZ09833"/>
        <property type="gene ID" value="Metbo_1606"/>
    </dbReference>
    <dbReference type="GeneID" id="10278063"/>
    <dbReference type="KEGG" id="mel:Metbo_1606"/>
    <dbReference type="eggNOG" id="arCOG03110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047250_2_2_2"/>
    <dbReference type="OMA" id="SRMEFAK"/>
    <dbReference type="OrthoDB" id="55690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="102"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="39664" checksum="0A6E984D68284668" modified="2011-05-03" version="1">MFKPKFNYTNKMVNNLVEIQSIKDFIINSPLILETEVSLRRDAVLKAAHHSTAIEGNPLRMDQVEAIFKTMEKTSQRKSDQEVLNYINVLKNLENYLVNGKIVEKTILNLHEGITHYTLDYTYLEGQYRIVPVHIINKEGETVFTPPPVNSIKKSIGDLVKWINNSEELNPVIASGILHYEFVRVHPFIDGNGRTGRVLTALYLHTRKFDMDRMFTLDEYYDKNREDYYSALNSVDQKTQDLTHWLDYFIEGFKHSLLKIKDQLLLISPEKSKENRVKLSEKNRKILEYIHLNGSITNSKVQNLLNISRQGAYKDLRYLMDENIIEQQGGSRSTYYVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E3</accession>
    <name>F0T9E3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1668</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09894">
        <property type="protein sequence ID" value="ADZ09894"/>
        <property type="gene ID" value="Metbo_1668"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1668"/>
    <dbReference type="HOGENOM" id="CLU_2662359_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="75" mass="8603" checksum="292AED0FB939D049" modified="2011-05-03" version="1">MVKRMVKTNPSIDKDMNLEEVTEELFILVRNCYNVGNNDIKLIVLDNCMKIILDSLENPDDLKQLKSVFDSITGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H2</accession>
    <name>F0T9H2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644071.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0468"/>
    <dbReference type="EnsemblBacteria" id="ADZ08720">
        <property type="protein sequence ID" value="ADZ08720"/>
        <property type="gene ID" value="Metbo_0468"/>
    </dbReference>
    <dbReference type="GeneID" id="10276906"/>
    <dbReference type="KEGG" id="mel:Metbo_0468"/>
    <dbReference type="eggNOG" id="arCOG01502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_2_2"/>
    <dbReference type="OMA" id="ILCRLCM"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="156" mass="17596" checksum="4232996095CCC158" modified="2011-05-03" version="1">MKTLMVVDPRMCTECKDCVTACKEEHGVSRVKKTSTVPLFCMHCHPDKAPCARICPTNAIKEEDGVLMVDEDACILCRLCMISCPVGMMVMDLEKKSTQKCTLCFEKEDRILPACVEACKDNVLKVFSVEDLEDLKDDTTFADIIEEAMKIYQEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I8</accession>
    <name>F0T9I8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit</fullName>
            <ecNumber evidence="8">4.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0484</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644087.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0484"/>
    <dbReference type="EnsemblBacteria" id="ADZ08736">
        <property type="protein sequence ID" value="ADZ08736"/>
        <property type="gene ID" value="Metbo_0484"/>
    </dbReference>
    <dbReference type="GeneID" id="10276924"/>
    <dbReference type="KEGG" id="mel:Metbo_0484"/>
    <dbReference type="eggNOG" id="arCOG04407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_2"/>
    <dbReference type="OMA" id="IETYPTH"/>
    <dbReference type="OrthoDB" id="59419at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="9"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="282" mass="30437" checksum="BF1BB83204285BC3" modified="2011-05-03" version="1">MITQKTVEDTICRLYRDSVIQLPEDVKNALREAHENETNETARLNLGAILENIDAAHENDIPMCQDTGLPIVFVKLGDVKVENLYEGIKKGVEKATTKVPLRPNVVDPFTRVNTGTNTGKNIPLVDIELVEGNNLELTIFPKGFGSENNNALKMALPGEGMEGVKEFVMETVLKAGGKPCPPTRIGVGIGGSSDYALKLAKKALLRDVKSVNPDERLAKLEEELIEQINSTGIGPMGLGGKTTALDVKVELADTHTAGLPIGVCVQCWAARHATAVLEDPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K2</accession>
    <name>F0T9K2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphogluconate dehydrogenase, NAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644101.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0498"/>
    <dbReference type="EnsemblBacteria" id="ADZ08750">
        <property type="protein sequence ID" value="ADZ08750"/>
        <property type="gene ID" value="Metbo_0498"/>
    </dbReference>
    <dbReference type="GeneID" id="10276938"/>
    <dbReference type="KEGG" id="mel:Metbo_0498"/>
    <dbReference type="eggNOG" id="arCOG00247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052530_1_0_2"/>
    <dbReference type="OMA" id="ASATKMC"/>
    <dbReference type="OrthoDB" id="82121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
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        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P5C_Rdtase_cat_N"/>
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        <property type="entry name" value="Pgluconate_DH_NAD-bd_C"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1932" evidence="2">
        <location>
            <begin position="182"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="29904" checksum="B259AF888600041D" modified="2011-05-03" version="1">MKIGFLGFGEVATTFSSGLKENGLDVYTCLNGRSSRTVENAVNSGVKLCNTHRELAEKSDILFSSVVPSMAVEVAKTVGNHSNGIYVDINNISPSTVKTALSKIKNGRTVDAAIIGSVKRNGLKVKIIASGTSAEEILNLNQHGMNITSVGQEAGDASTIKLLRSAYTKGVSALLYESLYHAYKLGINEEVLECISETETGDFIESANSRIVSASFHAKRRAEEMDEIVEFLSEHQNPIMAQATSKFFKNLSETIQKPSKRPENYTEVYKLLNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P3</accession>
    <name>F0T9P3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645273.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1697"/>
    <dbReference type="EnsemblBacteria" id="ADZ09922">
        <property type="protein sequence ID" value="ADZ09922"/>
        <property type="gene ID" value="Metbo_1697"/>
    </dbReference>
    <dbReference type="GeneID" id="10278154"/>
    <dbReference type="KEGG" id="mel:Metbo_1697"/>
    <dbReference type="eggNOG" id="arCOG01744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_878819_0_0_2"/>
    <dbReference type="OMA" id="FIFSHRG"/>
    <dbReference type="OrthoDB" id="73037at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="YdjM-like"/>
    </dbReference>
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        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="35713" checksum="01B0A115F5CE1413" modified="2011-05-03" version="1">MDLFTHFLVPYIILYALKSKNKLAGAFGGISPDFDTIFIAWIGILSPNFFIFSHRGITHSFIFGFVTSTIFLYVISRKQVNEFIGNLIRRDISVKFTKTTVAIAYFGVLTHLFLDYLTTLGIPLFFPFSITRYALNLYQSLDALTIVIALVVLIIIYLKVDTKYKKAAMAIFMIILISFGGVRAYEKINIMDHETPTLYGNYSELDVYPTNDMFVWNVVKHNPDNTSFAVSVYNGWYNNQYNLNYYSSPLITGGDYNSGLKAIKIADSYPSVKQFKWNSFFALENASYNGNSWDVTYYDVVKSYTKSNVTVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA40</accession>
    <name>F0TA40_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645341.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1767"/>
    <dbReference type="EnsemblBacteria" id="ADZ09990">
        <property type="protein sequence ID" value="ADZ09990"/>
        <property type="gene ID" value="Metbo_1767"/>
    </dbReference>
    <dbReference type="GeneID" id="10278224"/>
    <dbReference type="KEGG" id="mel:Metbo_1767"/>
    <dbReference type="eggNOG" id="arCOG01917">
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    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051619_0_0_2"/>
    <dbReference type="OMA" id="DANDFLM"/>
    <dbReference type="OrthoDB" id="78924at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zinc_ribbon_2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="337" mass="37385" checksum="4561D6A90E902209" modified="2011-05-03" version="1">MFCPNCGAKNPADARFCNQCGTHLTESMNDPTEDPDQDSGLESDTLNSTHEDGETEDSAVHCLICKTGSMHPTIHSGTFGFGTRKMLICNNCGAGFEIKGQKYKFSEISDTTQPLWLRYGHQTLTEAEWIRISEGGVSDEEQKKINREAEALKKQEIAKQKEKDANDFLMGLENGSIPINYSGQSPVILKKDELISIIMENINLQEPRAVRQTNAAYGGPTIRVAKGVSFRMGGASARSESYEEIRVIDNGKLVLTNKRLIFIGTKRTVNIDLRKIMAIVPYKDGIESQRENKQKPEYFTGTDKHTINYTINGRSGTVPIYGNVLKAAIQGLISKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA50</accession>
    <name>F0TA50_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645351.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10000">
        <property type="protein sequence ID" value="ADZ10000"/>
        <property type="gene ID" value="Metbo_1777"/>
    </dbReference>
    <dbReference type="GeneID" id="10278234"/>
    <dbReference type="KEGG" id="mel:Metbo_1777"/>
    <dbReference type="HOGENOM" id="CLU_2857108_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7431" checksum="6FABEEB29B8CDE05" modified="2011-05-03" version="1">MDENKKKKKETGDDMWSSAEEDMKERAPPDLLKKEDPELYKKEYGDTKVNPKDSGGKKLESKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA94</accession>
    <name>F0TA94_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1822</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645395.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1822"/>
    <dbReference type="EnsemblBacteria" id="ADZ10044">
        <property type="protein sequence ID" value="ADZ10044"/>
        <property type="gene ID" value="Metbo_1822"/>
    </dbReference>
    <dbReference type="GeneID" id="10278279"/>
    <dbReference type="KEGG" id="mel:Metbo_1822"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_6_0_2"/>
    <dbReference type="OMA" id="MIDKKEM"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="103"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="23676" checksum="1FDDEECFB92CE59B" modified="2011-05-03" version="1">MDKTSKTKEKAIEVKKGALEKTLDAKDKALDKTLEAKDKALMKTSQIKKDTGTKTAAIKDEASEKTTELKENTEKTRKQAENKINDFINSLKGKQDELGKTLSDYATGEKPLTDIISSPESFIIKADLPGVSKEDVSVHITEDLVEITAKFEDYPEDADFIKKERNYGEVNRKIKLPELVEVKKASAKFEDSVLTIELPKVKEDKIKIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA99</accession>
    <name>F0TA99_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10049">
        <property type="protein sequence ID" value="ADZ10049"/>
        <property type="gene ID" value="Metbo_1827"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1827"/>
    <dbReference type="HOGENOM" id="CLU_1093182_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="30981" checksum="2381F25C5165746C" modified="2011-05-03" version="1">MKVLDCESFHTTLLSLESITHVKQESILSFLNNFDMDDFYYRNPLYGSGDELLLLKFKNEFDPVTSYDQSNWFHLTRLYKNDDFKDGILPLVDVVEDKWDKLYSLISDKVSTEEWTDFRKNMEIGALGPHSSLYKFRMSGTDFKGPYAISIKETAFVSDQIRNHDYLAVPEIIKDICFCFEQTFGYDLFKEYTKNSEPCIVKFKAEYSHPNLIGKVLFYLYQRVHEKELNSNCKSNYNANGNKIPPEDILKVEYLNMGTIIDGSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB0</accession>
    <name>F0TAB0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">50S ribosomal protein L7Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl7ae</name>
        <name evidence="10" type="ordered locus">Metbo_0611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644213.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0611"/>
    <dbReference type="EnsemblBacteria" id="ADZ08862">
        <property type="protein sequence ID" value="ADZ08862"/>
        <property type="gene ID" value="Metbo_0611"/>
    </dbReference>
    <dbReference type="GeneID" id="10277056"/>
    <dbReference type="KEGG" id="mel:Metbo_0611"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OMA" id="KPFYVRF"/>
    <dbReference type="OrthoDB" id="114541at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_L7Ae_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_L7Ae/L8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_L7Ae_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000948">
        <property type="entry name" value="Ribosomal_L7Ae_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="9">
        <location>
            <begin position="17"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="13242" checksum="AF6C34A6433B124D" modified="2011-05-03" version="1">MAKAIYVKFDTPKEIADKAYEALEIAKDTGKIGKGTNEVTKMIERGNALLVFIAEDIDPPEIAAHLPVLAEEKEIPYVYLPTKDELGEAAGLNVGTASACIIDAGEAEDLINDVVEKVAELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE4</accession>
    <name>F0TAE4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="10" type="ordered locus">Metbo_0645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644247.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0645"/>
    <dbReference type="EnsemblBacteria" id="ADZ08896">
        <property type="protein sequence ID" value="ADZ08896"/>
        <property type="gene ID" value="Metbo_0645"/>
    </dbReference>
    <dbReference type="GeneID" id="10277090"/>
    <dbReference type="KEGG" id="mel:Metbo_0645"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="OMA" id="GTQRQMM"/>
    <dbReference type="OrthoDB" id="111803at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="18"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="33765" checksum="760E8F2B5306EE42" modified="2011-05-03" version="1">MEIVFLGTSSAIPTNNRNHSALALKAFGEIILFDCGEGTQRQMTRARLSPMKIDRIFVTHFHGDHILGIPGLIQSMAFRGRRDTLEIYGPPGIQELLNSMKKLGYFALSFNIAVHEIKDGTVLEEDDFKISAQLMKHSVENYAYCIEEKRDPKFIKEKALKLGIKPGPAYGKLQSGLPVKIGNKIIKPEEVLGEKRRGRKLVYSGDTLACEEMVGFSKDADVLIHESTFNNSHKEKAFETGHSTAEMAADIAKKAGVKSLVLTHISTRYKDVKTLEMEALNVFEDSVVAEDLMRLEVEQHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF7</accession>
    <name>F0TAF7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0658</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644260.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0658"/>
    <dbReference type="EnsemblBacteria" id="ADZ08909">
        <property type="protein sequence ID" value="ADZ08909"/>
        <property type="gene ID" value="Metbo_0658"/>
    </dbReference>
    <dbReference type="GeneID" id="10277103"/>
    <dbReference type="KEGG" id="mel:Metbo_0658"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_2"/>
    <dbReference type="OMA" id="DLWKGRV"/>
    <dbReference type="OrthoDB" id="98664at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="5"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="290" mass="31535" checksum="A33B0BDED44C97D7" modified="2011-05-03" version="1">MNQLDVLALGTCNIDFLMNVPRFAVADDEVDTEKLNVTLGGSAANFALKTSDLGLKTGIMARIGKDNFGNYIRSNFKEKNINTERLITIDEKTGMAFIAVDQVGERSIYTYMGANSKFELLKPDINLIKNSEILHLTGMYIEVVEEASKHANTLSFNPGALLASFGMKAMEDVLSRTDILFLNEKEVGLLTGENCPSGAKLLVEAGVKMVVVTLGKAGSKLFTMDHEIHQPSNMVKPVDTTGAGDSFAAGFINGFLRNRNLPNCLKDGNNSAFDCLTKFGASDTDLFKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR7</accession>
    <name>F0TAR7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAM_MT_TRM5_TYW2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0692</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ08943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_0692"/>
    <dbReference type="EnsemblBacteria" id="ADZ08943">
        <property type="protein sequence ID" value="ADZ08943"/>
        <property type="gene ID" value="Metbo_0692"/>
    </dbReference>
    <dbReference type="GeneID" id="10277137"/>
    <dbReference type="KEGG" id="mel:Metbo_0692"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OMA" id="HYYDIQH"/>
    <dbReference type="OrthoDB" id="55502at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
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        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="Trm5_N"/>
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    </dbReference>
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        <location>
            <begin position="95"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="38662" checksum="6ABE3963924F0523" modified="2011-05-03" version="1">MLGLKVSKNNADEIRRTLLKNSLIDLGWKIKTSDEHVYIPINKVPSEDLIKELDGFEIEVVDTDFKIHKKHPKSFKDYLKNTVEADKVDEIKNSFDIMGDVVVLEIPDEFEAERFLIGEAALKFTKRKAIYRKTSEIKGVIRTRELEHIAGEDVSETVHKEFGSRIMLDVRRVYFSPRLATERRRVAEQVEDGELIIDMFAGVGPFPVTIAKDHDVKIYAVDINPAAHHYIKRNIEINKLKGEIIPILGDVKTVLNDLNLSADRIIMNLPGTACDFLSTAINSLKSGGVLHYYEFASDYKVPVERIVETAKPRNVEILNVRKVKSRSPGVWHMAVDAKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS3</accession>
    <name>F0TAS3_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Euryarchaeota</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644300.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0698"/>
    <dbReference type="EnsemblBacteria" id="ADZ08949">
        <property type="protein sequence ID" value="ADZ08949"/>
        <property type="gene ID" value="Metbo_0698"/>
    </dbReference>
    <dbReference type="GeneID" id="10277143"/>
    <dbReference type="KEGG" id="mel:Metbo_0698"/>
    <dbReference type="eggNOG" id="arCOG02702">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="OMA" id="MTYHEFA"/>
    <dbReference type="OrthoDB" id="67424at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Methyltranfer_dom"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="30"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="20951" checksum="FB06F2B9731D0637" modified="2011-05-03" version="1">MSEENVHRHHGKTSRDILGAEEILDVINLKSGDKFLDAGCGDGYVSIEASKLVGSNGKIFALDVYPESIEGVAKEVKEKGIENLEPILADVTDSIPLDDDSVDAVLMSNVLHGFGDEELETVLKNINRVIKKDGKFAVVEFRKVQGERGPPFDVRLHPSDVSQILSKHGYKVVKSQEIATLHYIVVGQKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW2</accession>
    <name>F0TAW2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTP-binding protein HSR1-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0737"/>
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        <property type="protein sequence ID" value="ADZ08988"/>
        <property type="gene ID" value="Metbo_0737"/>
    </dbReference>
    <dbReference type="GeneID" id="10277184"/>
    <dbReference type="KEGG" id="mel:Metbo_0737"/>
    <dbReference type="eggNOG" id="arCOG00354">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399745_0_0_2"/>
    <dbReference type="OMA" id="ATKIDFH"/>
    <dbReference type="OrthoDB" id="61585at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="25"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="23807" checksum="FE76B4D21F60A7F4" modified="2011-05-03" version="1">MPKFLKRNFLRNIFNKLIGKDKKLKIGLYGHPNSGKTTLANTMTNDWLGKPIGLISEIPHETRRVYRQERVKIEYDDVELDFDIVDTPGIATKIDYKNFLQFGLSEQEAKERAKEATKGIIEAIKWLDDVTGVLLVVDATQDPLTQANITIMGNLEARKIPFVIVANKIDLPEASAERITSVFPQHTVVPISALHGENTGDLYQAMVEKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX2</accession>
    <name>F0TAX2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0747"/>
    <dbReference type="EnsemblBacteria" id="ADZ08998">
        <property type="protein sequence ID" value="ADZ08998"/>
        <property type="gene ID" value="Metbo_0747"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0747"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_4_2"/>
    <dbReference type="OMA" id="DECITAM"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041359">
        <property type="entry name" value="MetOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18546">
        <property type="entry name" value="MetOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="8"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="33111" checksum="B9CD5F5BCD06ADC1" modified="2011-05-03" version="1">MVAIPEEKILIIEDENITALEIQNKLDKWGYDVVGTAGSGEEAIQIANENEIELILADIVLRGDMDGIDAVDRISDRYDVPVIYLTAHADNDTFSRAKMTKPSGYVIKPFDDTELKYSIEIALLRNDYADKAVQAKDKERMATVRDFMLSSTPALTSQVRIQDTAKFLKDFANFFETNMHHKMNRELKLDDDSYKNTDNSRKILTEYIAWVTNMFTNLGYRIETGSDEFTITECLWSPNVNDNKIYCLMCRAMVELSLNWTDIDVKMFHSYLLGPNPPKCRFSFNRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB00</accession>
    <name>F0TB00_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Histone acetyltransferase, ELP3 family</fullName>
            <ecNumber evidence="14">2.3.1.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Metbo_1926</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + carboxymethyluridine(34) in tRNA + CoA + 2 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="14"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645497.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1926"/>
    <dbReference type="EnsemblBacteria" id="ADZ10146">
        <property type="protein sequence ID" value="ADZ10146"/>
        <property type="gene ID" value="Metbo_1926"/>
    </dbReference>
    <dbReference type="GeneID" id="10278384"/>
    <dbReference type="KEGG" id="mel:Metbo_1926"/>
    <dbReference type="eggNOG" id="arCOG01361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="OMA" id="TFETRPD"/>
    <dbReference type="OrthoDB" id="7724at2157"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="PTHR11135:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="67"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="542" mass="62504" checksum="584EE16CDBA6D615" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB01</accession>
    <name>F0TB01_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="4">DERA</shortName>
            <ecNumber evidence="4">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="4">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">deoC</name>
        <name evidence="5" type="ordered locus">Metbo_1927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645498.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1927"/>
    <dbReference type="EnsemblBacteria" id="ADZ10147">
        <property type="protein sequence ID" value="ADZ10147"/>
        <property type="gene ID" value="Metbo_1927"/>
    </dbReference>
    <dbReference type="GeneID" id="10278385"/>
    <dbReference type="KEGG" id="mel:Metbo_1927"/>
    <dbReference type="eggNOG" id="arCOG04320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_0_2_2"/>
    <dbReference type="OMA" id="MNACIPP"/>
    <dbReference type="OrthoDB" id="74992at2157"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="PTHR10889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="PTHR10889:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="4">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="23074" checksum="80073B05079A7F00" modified="2011-05-03" version="1">MMTPEKLAKMIDHTNVKPNASTEDINALCEEASEYNFACACVTPVNVALASELLKGTDVGVCAVIGFPFGTSKSEIKAFEALVAVEDGANELDMVLNIGALKSGLSRMVERDIEHVVESAEGMVVKVIIETALLTDDEKVEACMLAKSAGADYVKTSTGVGFPGANAADVKLLRETVGQKMGVKASGGIRNLKSALEMIEAGASKIGTSAGPSIMNGFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB07</accession>
    <name>F0TB07_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645504.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10153">
        <property type="protein sequence ID" value="ADZ10153"/>
        <property type="gene ID" value="Metbo_1933"/>
    </dbReference>
    <dbReference type="GeneID" id="10278391"/>
    <dbReference type="KEGG" id="mel:Metbo_1933"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_1_0_2"/>
    <dbReference type="OrthoDB" id="82596at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="17925" checksum="1539A7F7A4D728C2" modified="2011-05-03" version="1">MSSKENLMEAFAGESQANRKYLAFAKKADEEGFSQVAKLFRAAAAAETVHAHNHLEIAGGINDTEANLKDAIEGETFEFTTMYPNFIAEAKAEKEDAARWTFDVANSVEQIHADLYKNALENLGKNSDVDYFVCALCGNTVENEAPEKCPICGAENSEFMKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB21</accession>
    <name>F0TB21_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CDP-archaeol synthase</fullName>
            <ecNumber evidence="6">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">carS</name>
        <name evidence="7" type="ordered locus">Metbo_1947</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645518.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1947"/>
    <dbReference type="EnsemblBacteria" id="ADZ10167">
        <property type="protein sequence ID" value="ADZ10167"/>
        <property type="gene ID" value="Metbo_1947"/>
    </dbReference>
    <dbReference type="GeneID" id="10278407"/>
    <dbReference type="KEGG" id="mel:Metbo_1947"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="OMA" id="GKTWRGT"/>
    <dbReference type="OrthoDB" id="124973at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="PTHR39650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="172" mass="18268" checksum="3AF4E6CFF86FD2CF" modified="2011-05-03" version="1">MDSSVVSVINLSAYAIYFMLPAYLANVTALAFGGGKPLDFNHKFRDGRRLIGNGVTVRGTIIGTLIGTLVGVVQGILTGNIIEGILLGFTLGAGALIGDACGSFIKRRLNIGRGKPAPLLDQLDFVVGALVFASLIVILPLEMIVIIIVLTLFLHLGTNIIAYLLGIKEVWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB36</accession>
    <name>F0TB36_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645533.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1962"/>
    <dbReference type="EnsemblBacteria" id="ADZ10182">
        <property type="protein sequence ID" value="ADZ10182"/>
        <property type="gene ID" value="Metbo_1962"/>
    </dbReference>
    <dbReference type="GeneID" id="10278422"/>
    <dbReference type="KEGG" id="mel:Metbo_1962"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OMA" id="DPSEYCG"/>
    <dbReference type="OrthoDB" id="66209at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="163"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="37467" checksum="B8C9D724937EED5D" modified="2011-05-03" version="1">MFIPTIPTPEEVLDKSFSRAKKAANKVRGSKIPRHQKSKKTEEARIKTACQVTQETFNTILEKVPKVEDLNMFYQDYIDVVVGVDQFKKSLGALKWASELIAKFENQYVFKMRRSSSENASNVRKEAFGRLASVVYRIEDELNFLDFAKQKLRNMPTIDFDATTAVIAGFPNVGKSTLLRQLTSAEPKVADYPFTTTGIQIGHIEHKWMPYQFIDTPGLLDRPVDDMNEIELNAMVALEHLADLVFFIFDASETSGFPLESQMKLYHGIKSIFNTPIQCVFNKMDLVEDTTLVDKYIEQLDNPIKLSAVEGIGVSELITKMEEFKNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB38</accession>
    <name>F0TB38_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1964</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645535.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1964"/>
    <dbReference type="EnsemblBacteria" id="ADZ10184">
        <property type="protein sequence ID" value="ADZ10184"/>
        <property type="gene ID" value="Metbo_1964"/>
    </dbReference>
    <dbReference type="GeneID" id="10278424"/>
    <dbReference type="KEGG" id="mel:Metbo_1964"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="OMA" id="SVFHDKM"/>
    <dbReference type="OrthoDB" id="22382at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="116"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="229"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="49257" checksum="B4D890C7040F1374" modified="2011-05-03" version="1">MPSSKENNLDPDLFRKTIEIVEKKVDFVDIRVSESKNNVIVMKDGQIQEIRSGTDFGGSVRVLKNGSWGFAYTTQLSRLDEMAEAALKLSNSLKSDVEVSKTEGSTDSVKSKAKIPLSSVSLDEKKKVMEEAEAAANIDKVVSTTVNYVDGESSSLYMNSEGSAISMEESRVAMFLNAVAASEGVIQFGHKSIGGSKGYEAIQNQDIEEFGRLAATKAVRLLDAKSPPSGSFPVVLDPELTGVFIHEALGHASEADLILQNDSILKNKMGTQIGSDLVTIVDDASMDAFGYYAYDAEGIKTRENILVQDGVLSSLLSSRETASKLKMESSGNARSSVGDQPIVRMSNTYLKPGDQSLEELLEGIKDGIYLKGSRGGQVDTGKGIFQFNAAESFVVENGEIKDPLRDVSLSGNILEILNHIDGLGSDFKMGVGFCGKNGQTVPVGDGGPHVRVSKATVGGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB46</accession>
    <name>F0TB46_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645543.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1972"/>
    <dbReference type="EnsemblBacteria" id="ADZ10192">
        <property type="protein sequence ID" value="ADZ10192"/>
        <property type="gene ID" value="Metbo_1972"/>
    </dbReference>
    <dbReference type="GeneID" id="10278432"/>
    <dbReference type="KEGG" id="mel:Metbo_1972"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070562_1_1_2"/>
    <dbReference type="OMA" id="PIKAPHY"/>
    <dbReference type="OrthoDB" id="66283at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029478">
        <property type="entry name" value="TM1586_NiRdase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14512">
        <property type="entry name" value="TM1586_NiRdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TM1586_NiRdase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="255" mass="29202" checksum="D58C05276DA5304C" modified="2011-05-03" version="1">MDKNEIYETIFRRKSIRKYDQDTLDEEILKDIQGEVMDLKPLIPGIKTEVLLISSEDVKTRMMKKAPHYLAVFSEEKEGYLTNVGYMLQQMDLLFSANGIGCCWQGIPTIKDHVKPRSELKFVILLAFGNPDEPLHRSDISEFKRNPIEKMTDITITDENREILEAGRLSPTATNSQAWFLSGDDSCIDVFTTKPNFIRSLIAKKYIPIDAGIVIYQIMVAATHFKQNPEVVFKEGIEKKGYIYCASINLNPDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB63</accession>
    <name>F0TB63_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/acetyl-CoA-carboxylase ligase</fullName>
            <ecNumber evidence="2">6.3.4.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.4.15" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644365.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0763"/>
    <dbReference type="EnsemblBacteria" id="ADZ09014">
        <property type="protein sequence ID" value="ADZ09014"/>
        <property type="gene ID" value="Metbo_0763"/>
    </dbReference>
    <dbReference type="GeneID" id="10277211"/>
    <dbReference type="KEGG" id="mel:Metbo_0763"/>
    <dbReference type="eggNOG" id="arCOG01940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_0_0_2"/>
    <dbReference type="OMA" id="AVWKHIE"/>
    <dbReference type="OrthoDB" id="42041at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00978">
        <property type="entry name" value="Bifunct_BirA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030855">
        <property type="entry name" value="Bifunct_BirA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="67"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="323" mass="36370" checksum="EEBB5C30329D3B42" modified="2011-05-03" version="1">MTKRKILKTLYEKKDEYVPLEQFVNETGKSPEIIENEFLNLEEEGYIINHSKLGYRLIKTPNLLLPYEIKRGLKTNFMGHDIHYFNEVDSTNEVAKYLAEEGAEDGTVVVAEIQNKGKGRRGKTWISPPGGIWMSVILRPEIPPHRASQLTLVTGVVVAKTIKEELNIDVGIKWPNDILIGKKKVCGILTEVNATLDKVNYLVVGIGIDMNVDVPMLPSDLQKGATSLKNELNTEINGALLVQRFLQNFENIYNDFKDGKFPEILDEWRAMSSTIGTSVEVRTRGKRVQGYAVGINKEGILIIEMEDGSLRKMISGECLHLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB95</accession>
    <name>F0TB95_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps5</name>
        <name evidence="8" type="ordered locus">Metbo_0795</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644397.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0795"/>
    <dbReference type="EnsemblBacteria" id="ADZ09046">
        <property type="protein sequence ID" value="ADZ09046"/>
        <property type="gene ID" value="Metbo_0795"/>
    </dbReference>
    <dbReference type="GeneID" id="10277243"/>
    <dbReference type="KEGG" id="mel:Metbo_0795"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="60954at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_S5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_S5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="7">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="215" mass="23217" checksum="D86E5A16BBEFC95A" modified="2011-05-03" version="1">MNNNYNKEEFVPKTQLGHMVKEGKITDIDEIFDKGLPIMELGIIDTLLPDLEEEVMDVNLVQRMHKSGRKVNFRVIVAVGNKNGYIGLGQGKAKEVGPAIRKAVDNAKFNIIKVRRGCGDWGCVCGREHTVPFKVEGKKGSVRVTLIPAPGGVGLAIGNVGKTILGLAGIDDVWSQTMGQTQTTINFAGAVFEALKELSKVKATSKDLKNLGVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA5</accession>
    <name>F0TBA5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps13</name>
        <name evidence="7" type="ordered locus">Metbo_0805</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644407.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0805"/>
    <dbReference type="EnsemblBacteria" id="ADZ09056">
        <property type="protein sequence ID" value="ADZ09056"/>
        <property type="gene ID" value="Metbo_0805"/>
    </dbReference>
    <dbReference type="GeneID" id="10277254"/>
    <dbReference type="KEGG" id="mel:Metbo_0805"/>
    <dbReference type="eggNOG" id="arCOG01722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_1_2"/>
    <dbReference type="OMA" id="IRAYRGI"/>
    <dbReference type="OrthoDB" id="84219at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_S13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17258" checksum="D35E197899763C99" modified="2011-05-03" version="1">MEEEFKHMVRISRKDVDGKKTIENALADIKGIGRALSRALCQVAGFDATMQIGYLPDKDVLKLEEAVKAPQQFELPEWMLNRRNDYETGETVHLIESDLTMRLRDDLNRMKKTRSYKGRRHEVGLPVRGQRTKSTFRKGKSVGVRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA6</accession>
    <name>F0TBA6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps4</name>
        <name evidence="10" type="ordered locus">Metbo_0806</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644408.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0806"/>
    <dbReference type="EnsemblBacteria" id="ADZ09057">
        <property type="protein sequence ID" value="ADZ09057"/>
        <property type="gene ID" value="Metbo_0806"/>
    </dbReference>
    <dbReference type="GeneID" id="10277255"/>
    <dbReference type="KEGG" id="mel:Metbo_0806"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OMA" id="ARQFITH"/>
    <dbReference type="OrthoDB" id="93256at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_S4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_S4/S9_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_S4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="9">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="8">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="20498" checksum="1C483B86DCCBC269" modified="2011-05-03" version="1">MGHPRKARKQYDTPSHPWNAARIKEENKLVTKFGLRTKKEVWKAETIVKRYRRDARLLLGLETEHAEVEKAQLLNHIKRLGMLKADSKLEDILDLTVEDVLRRRLETIVYKRGLSNTAKQARLFIVHGHIAMNGRKINAPGYLVKSGEEETIGFYPGSPMEKIQKEQAKTQAAEEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF2</accession>
    <name>F0TBF2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645572.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2002"/>
    <dbReference type="EnsemblBacteria" id="ADZ10221">
        <property type="protein sequence ID" value="ADZ10221"/>
        <property type="gene ID" value="Metbo_2002"/>
    </dbReference>
    <dbReference type="GeneID" id="10278462"/>
    <dbReference type="KEGG" id="mel:Metbo_2002"/>
    <dbReference type="eggNOG" id="arCOG00480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904930_0_0_2"/>
    <dbReference type="OMA" id="QLFIVYA"/>
    <dbReference type="OrthoDB" id="73386at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901663">
        <property type="term" value="P:quinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="PTHR13929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35124" checksum="817F28619D4598FA" modified="2011-05-03" version="1">MFRDVSVKSSNLKDLFKFIKLGKPQFAVGLFFYFSLGALLALLLGAEFNLTKFILGFVIIFFSTWAVHYHNDYYDFEADHYVTPTPISGGSGVLIEHPEWKKSSKYMAISLISIAILVSVVFTLLFDYPLTFILFVIFANILSWFYAAPPFKLSYRGLGEFGNTAIGILFPGLGFFALMGTLTLPFLAFAIPMLFLQMLFTMSVEIPDMEGDKAGGKMTWIATHGRRFGFKIIAVSGLLTTISFLILNYTNLFPDFIDFRVVAAISSIPLILGIIALIKNPEDKLTATKFCIYNLAGIFAVVILINIYFISLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI4</accession>
    <name>F0TBI4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2034"/>
    <dbReference type="EnsemblBacteria" id="ADZ10253">
        <property type="protein sequence ID" value="ADZ10253"/>
        <property type="gene ID" value="Metbo_2034"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2034"/>
    <dbReference type="eggNOG" id="arCOG00478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048113_0_0_2"/>
    <dbReference type="OMA" id="NIHMYLL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="287" mass="32332" checksum="D4AE36076B48D509" modified="2011-05-03" version="1">MAWILSVKKKLIIPDTMIKTLIKSTRISWAAKNVNMYLLVLTYAYFADAFISNPLEILEGLILVSALWGALYSLNDLTDIDEDMKDSMKQSRPFIQEHVEKKWIVAFCSVIIGAVFVVSILTLPLAFTVIVLLMFLNQVLYTVPPIRLKDTVLAPFFSTATNSVLRMASCAVLLGSIAIVPWSVYLFMYVGSMGTYIMYKSKQKMASVLTAITGIILLYAFINGDMNLIQFAVAVLPASFATLPLYLSLFTQKDKMFDIADVLYHQIAMIFFLICIFYILFFHSTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI9</accession>
    <name>F0TBI9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PUA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645609.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2039"/>
    <dbReference type="EnsemblBacteria" id="ADZ10258">
        <property type="protein sequence ID" value="ADZ10258"/>
        <property type="gene ID" value="Metbo_2039"/>
    </dbReference>
    <dbReference type="GeneID" id="10278500"/>
    <dbReference type="KEGG" id="mel:Metbo_2039"/>
    <dbReference type="eggNOG" id="arCOG00990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920138_0_0_2"/>
    <dbReference type="OMA" id="PFGICPR"/>
    <dbReference type="OrthoDB" id="50461at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PUA" evidence="3">
        <location>
            <begin position="235"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="304" mass="34445" checksum="588CF31951CE8E00" modified="2011-05-03" version="1">MKIPCTIDESLYRPEVVRWRERMSLLEPLGDVVVVLPCSMRKPYTNSKSHSVFMRATKGVQEAILTSPFGVCPREMDRTYPIQSYDTSTTGDWSHEEIDLTGECLKSYVDGKDVIAHVSGGYREACEAHLEDAIYTVEDGRATSWESMDNLKKELRNYPRIKNSQKRINGFRSIARYQFNTKKGDSFIPDETKAVGRFTRKMIYNHEQFATLSFDTGLYSLNLPGGEKVYDMGVNWVEIDFEMKTNTLFTPGVVDADPNIVPMDEVVVVRDGEVMAVGKSVLSGKEMKSATKGVAVKIRHRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL7</accession>
    <name>F0TBL7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0844"/>
    <dbReference type="EnsemblBacteria" id="ADZ09094">
        <property type="protein sequence ID" value="ADZ09094"/>
        <property type="gene ID" value="Metbo_0844"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0844"/>
    <dbReference type="eggNOG" id="arCOG02884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_686260_0_0_2"/>
    <dbReference type="OMA" id="INMAHTS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
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        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
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        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="155"/>
        </location>
    </feature>
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        <location>
            <begin position="201"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="471" mass="52990" checksum="64714F6AB5AFCA1D" modified="2011-05-03" version="1">MINKLKYLDLILIFITTIACLSVIMNPTLTNYIFRIILIIMLILFLSGYSLIAVIYPRQNDLDLLERLALSFGFPLIGIAIVIVLTNLIPLSYFNFLKSNINMIIGALGLLTIVLIFIAIIRRKKAIQENKYIYSRVYEVKNAIKPDLYEKSGSKNEKELISRNKEHKVIKNEIDDKSNLETKKRKNIYPKFVSKDLLLILLTSVLTIIFIVTPKLNETFVRTFLGILLILFIPGYSLIAALFPKKGDLDGIERSALSFGLSIAITPIIGLMLNYTPWGIRLTPILISLSAFTLIMLLIAFIRRRRVPEGQKFYVNFGGTLSSIKNSFSGESKTGKFLSIILIITILMAISTTVFIILKPKQGESFTEFYILGPNGKASDYPTNLTLGQNGTVIIGIVNHEYQTANYELVVSSNGTVMSQQNITLTNGNQTQIPYSFTAGSAGYKNIEFLLYKLPDTTNVYRSLHLYVNMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN5</accession>
    <name>F0TBN5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide pyruvyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644463.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0862"/>
    <dbReference type="EnsemblBacteria" id="ADZ09112">
        <property type="protein sequence ID" value="ADZ09112"/>
        <property type="gene ID" value="Metbo_0862"/>
    </dbReference>
    <dbReference type="GeneID" id="10277311"/>
    <dbReference type="KEGG" id="mel:Metbo_0862"/>
    <dbReference type="eggNOG" id="arCOG04826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039510_2_0_2"/>
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    <dbReference type="OrthoDB" id="84836at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="12"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="412" mass="47110" checksum="83F6A68C7AC5EA2F" modified="2011-05-03" version="1">MKILLTNVGEKNKGNSALIISTIETLKHYMPKTKFILIGGGNGEINNLKIEKQFSSELSIKKPDKTIKTFYYLFYCSLIRLIRILGFNCSIKKKSPLYYYDYVDVVINSGGDHFSGEYGIAVLNNFINLSYSIFLGKKVILYGESLGYYKNKNINWIARIVFNRVDLILVRDELSVKYLHDNHINNNKIAFTADPAFLLEVIPESEIVTILKSEKVDIKKPIVGMNPSGLISRFDKTECGNDEKIIQIFVKIIDYLIEEKNVNILLIPHDYTPYYDDRKVINSIFEKSNYKSSIFVINGEYDPRELKGIISKCDMFIGSRMHATIASTSSLVPTIGIAYSHKMKGIIGKMLGQEKYIIDINDFDYQKLLILVIDMWNNRADIKKELEIVIPLVKEKSSLNGKLVKELIMSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP3</accession>
    <name>F0TBP3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644471.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09120">
        <property type="protein sequence ID" value="ADZ09120"/>
        <property type="gene ID" value="Metbo_0871"/>
    </dbReference>
    <dbReference type="GeneID" id="10277320"/>
    <dbReference type="KEGG" id="mel:Metbo_0871"/>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800799_0_0_2"/>
    <dbReference type="OrthoDB" id="7003at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="110"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="346" mass="36697" checksum="FF0387778E7920D5" modified="2011-05-03" version="1">MQNNTILTGILIILCVLVTLIGYTNPTTSNILTIEPSTVVVNPGQSIQTAINSAPVGSTIIVNSGNYTENLKILKNITLTANGTVNLKQINIAYTGCKVKGFSLSGDYPIVITSTRNVEIIGNNISSNLNGIMSTGTNVNLKVSSNTLIGTNPFYGNNMAFEGPLYNSTIQNNVLSGAEFGMLYDAPSKNITINNNVVTNNYDLNNTRDPLVHTGTGIYTVAGSTDFKILNNTVINTRDSIAVEDLGGGLSTGFIIENNTVIKSFNGVWAKLSNSLIQNNNFSNNTAGIDVVGTGNIIKDNIIENNSVVGIALTTKQANDYNTLINNTLTGNGGPYYTVGPGRIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS4</accession>
    <name>F0TBS4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0902</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644502.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0902"/>
    <dbReference type="EnsemblBacteria" id="ADZ09151">
        <property type="protein sequence ID" value="ADZ09151"/>
        <property type="gene ID" value="Metbo_0902"/>
    </dbReference>
    <dbReference type="GeneID" id="10277351"/>
    <dbReference type="KEGG" id="mel:Metbo_0902"/>
    <dbReference type="eggNOG" id="arCOG04339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060703_2_0_2"/>
    <dbReference type="OMA" id="GFAWFQK"/>
    <dbReference type="OrthoDB" id="57185at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="17"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="29817" checksum="8AE7B0D5B4E57A69" modified="2011-05-03" version="1">MISDHRFIANFEKYRYLLVQLIKRDIQVKYRNSMLGIFWSFLEPLLTMIMFTIIFSYIFKRDIPNFPVYYLIGRVAFSLFQSGTTGAMKSIIGNSAILRSVYVPKYLFALASVGSAFVTFLLSLIILVAVMIVTNVHFTIYVIFASLPVLVLLLMTLGVGLILATVTVFFRDIEHLYGVFTMLLMYGSAVFYPASIIPAKWQFLLTINPFYAIISCMRTVLMNGTLYNTGELLYATVFSILCLVVGMGLFYKYQNKFLLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU6</accession>
    <name>F0TBU6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645639.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2069"/>
    <dbReference type="EnsemblBacteria" id="ADZ10288">
        <property type="protein sequence ID" value="ADZ10288"/>
        <property type="gene ID" value="Metbo_2069"/>
    </dbReference>
    <dbReference type="GeneID" id="10278531"/>
    <dbReference type="KEGG" id="mel:Metbo_2069"/>
    <dbReference type="eggNOG" id="arCOG01031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107938_2_0_2"/>
    <dbReference type="OMA" id="RTYNIIH"/>
    <dbReference type="OrthoDB" id="77148at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002800">
        <property type="entry name" value="Rv2949c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01947">
        <property type="entry name" value="DUF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="177" mass="20320" checksum="3AF01628EF45178E" modified="2011-05-03" version="1">MDKTILKSIEGIESEVGELSNAQKILLTTDGSITAILDVIKGHAKIETLEQKFIEADEKIAKLLDIEVGDEVNYRVVVIETNEPLIYAVSLIPIDRLEKDFKEDIIRADIPIGRILRKHKIESRREIKSVYSEKPSPEIGEIFNTDSVMLARTYNIIHHNEILIWLMETFPYSNFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU9</accession>
    <name>F0TBU9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylyl cyclase CyaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645642.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2072"/>
    <dbReference type="EnsemblBacteria" id="ADZ10291">
        <property type="protein sequence ID" value="ADZ10291"/>
        <property type="gene ID" value="Metbo_2072"/>
    </dbReference>
    <dbReference type="GeneID" id="10278534"/>
    <dbReference type="KEGG" id="mel:Metbo_2072"/>
    <dbReference type="eggNOG" id="arCOG01723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="OMA" id="QHPCRDF"/>
    <dbReference type="OrthoDB" id="123503at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="PTHR21028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="180" mass="20596" checksum="29392C56F0B7CDCA" modified="2011-05-03" version="1">MIEVEVKAKVKNFSDIKEKLIEMGAQKIKDEYQSDTYFNAHDRDFGVTDEALRIREIPENSGKRFILTYKGAKMDDLSKTRQEVEVDITDSENMALILINLGFKKAAVVTKDRSIYHLDEFIITLDTVHGAGIYVEIETDVEDGKDTTDSLNQIFEIYKTMGIEDGFERRSYLELLGAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY6</accession>
    <name>F0TBY6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="5">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glmS</name>
        <name evidence="8" type="ordered locus">Metbo_2109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645679.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2109"/>
    <dbReference type="MEROPS" id="C44.A08"/>
    <dbReference type="EnsemblBacteria" id="ADZ10328">
        <property type="protein sequence ID" value="ADZ10328"/>
        <property type="gene ID" value="Metbo_2109"/>
    </dbReference>
    <dbReference type="GeneID" id="10278572"/>
    <dbReference type="KEGG" id="mel:Metbo_2109"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_2"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="OrthoDB" id="7487at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
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    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
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        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 8" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="6">
        <location>
            <begin position="2"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="277"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="439"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="5">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="591" mass="65104" checksum="03645D7E1765B577" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC04</accession>
    <name>F0TC04_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase, TatD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2127"/>
    <dbReference type="EnsemblBacteria" id="ADZ10346">
        <property type="protein sequence ID" value="ADZ10346"/>
        <property type="gene ID" value="Metbo_2127"/>
    </dbReference>
    <dbReference type="GeneID" id="10278590"/>
    <dbReference type="KEGG" id="mel:Metbo_2127"/>
    <dbReference type="eggNOG" id="arCOG00891">
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    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
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    <dbReference type="OrthoDB" id="48998at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="28764" checksum="33A03BA486CC7353" modified="2011-05-03" version="1">MIDTHCHVDFKEYNKNREEVMERAKKKLTAIINSGASLGGNRRTLKLQDEYKGFLYSTLGFHPSNASKADSTIIKQVLEEIDTNIEKTVGIGETGLDFYEVKESDKRKRQLKIFDIFIELSNEYDLPLVLHARDAEAKCLEIVKKKSNIYDVIFHCYGGDVETAKQIVEEGYSISFSTLACFSEHHQELIEHVPISNILTETDSPYLSPFKGKRNEPAFVEETVKIIAEKKSLTPRAVGKQTERNARKIFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC14</accession>
    <name>F0TC14_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644516.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0916"/>
    <dbReference type="EnsemblBacteria" id="ADZ09165">
        <property type="protein sequence ID" value="ADZ09165"/>
        <property type="gene ID" value="Metbo_0916"/>
    </dbReference>
    <dbReference type="GeneID" id="10277365"/>
    <dbReference type="KEGG" id="mel:Metbo_0916"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011795_0_0_2"/>
    <dbReference type="OMA" id="VYHKLQK"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="96"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="335"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="599" mass="68650" checksum="26C8DDA4407C01E0" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC31</accession>
    <name>F0TC31_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Desulfoferrodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0935</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644533.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0935"/>
    <dbReference type="EnsemblBacteria" id="ADZ09182">
        <property type="protein sequence ID" value="ADZ09182"/>
        <property type="gene ID" value="Metbo_0935"/>
    </dbReference>
    <dbReference type="GeneID" id="10277384"/>
    <dbReference type="KEGG" id="mel:Metbo_0935"/>
    <dbReference type="eggNOG" id="arCOG02146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118960_1_0_2"/>
    <dbReference type="OMA" id="EEKHYIQ"/>
    <dbReference type="OrthoDB" id="106274at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00974">
        <property type="entry name" value="DSRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004462">
        <property type="entry name" value="Desulfoferrodoxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038094">
        <property type="entry name" value="Desulfoferrodoxin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004793">
        <property type="entry name" value="Desulfoferrodoxin_rbo"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06397">
        <property type="entry name" value="Desulfoferrod_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="SSF49367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00319">
        <property type="entry name" value="desulf_FeS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00320">
        <property type="entry name" value="dfx_rbo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Desulfoferrod_N" evidence="9">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Desulfoferrodox" evidence="8">
        <location>
            <begin position="44"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13849" checksum="A7E1F3D19E86E786" modified="2011-05-03" version="1">MERGQIYRCNTCGNMFELINVGGGTSSCCGTEMELLSDKAKDVGAEKHVPVIEKIDGGVKVKVGSVPHPMEEDHYIAWIEIITDNETQRAFLKPGEAPEAEFKIDDTTNLFAKEYCTVHGLWRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC69</accession>
    <name>F0TC69_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644571.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0973"/>
    <dbReference type="EnsemblBacteria" id="ADZ09220">
        <property type="protein sequence ID" value="ADZ09220"/>
        <property type="gene ID" value="Metbo_0973"/>
    </dbReference>
    <dbReference type="GeneID" id="10277422"/>
    <dbReference type="KEGG" id="mel:Metbo_0973"/>
    <dbReference type="eggNOG" id="arCOG01411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_27_1_2"/>
    <dbReference type="OMA" id="PIFYNAC"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="89"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="197"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="390" mass="45240" checksum="A0196E0CE82543CB" modified="2011-05-03" version="1">MEIDYINGVESSKIFGRSKYQMEIFGRINDVNLNIIEYKPLADILKKRLNMTPERSGAVTDNFQGGEPGRVSSFLFKTAFRMMDRMDQYRYIHEIKGKLKEENIKHITTQELAYVLKSLENYKTVLTCFDLIPWVYDNDRSYIWKENMKWMPTADRIITISEFSKNEILKYLDYPEEKIDIVYPAVDHRLYQPIKSKEILKQFNISDRDKVVLYVGSETPRQNVPDLIRAFNKLKKVMPNVKLLKIGESQSNDARVELLKLIKQQDLVDDVIFAGYVAEPEMPEWYSAADLLVYPCEYAGFGLPPLEAMACGTPVITSNTTSLPEVVGEAGIMLDPHDNEKLSKEMYRVLNDEKLADNLIQMGLERAKLFDWDESAQQTMEVYRSMEKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC77</accession>
    <name>F0TC77_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48 Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644579.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0981"/>
    <dbReference type="EnsemblBacteria" id="ADZ09228">
        <property type="protein sequence ID" value="ADZ09228"/>
        <property type="gene ID" value="Metbo_0981"/>
    </dbReference>
    <dbReference type="GeneID" id="10277430"/>
    <dbReference type="KEGG" id="mel:Metbo_0981"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584781_0_0_2"/>
    <dbReference type="OMA" id="PPIYFRI"/>
    <dbReference type="OrthoDB" id="36531at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="270"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="473" mass="53753" checksum="EE6A75D2E56F348B" modified="2011-05-03" version="1">MTRTYKFAIDTAVAQAHMKEVLEFIYKYYLLSKPKNFSSVNKINGSNNSKLSFYAINPEYGWKILVEIVAGSPLDVTITSYNGTPEKFVTNVKEELILAVQFFHESIRQSTLYFAWVEGEDIIPEEPPSSGKKLADRLFGSNLLFLYVIFFGINILFFIFLGLVFAVIAILGLQLIIVLLSDRIYLARNNWRITPSNPYVHIIEYQLPVEEFRLFKEKFGNDVVVQMKNDIYKRSLAIGKEPSCEIGEEIFEDYGFKCSPDSSLVKIVDVYSIVKTAASKFNIPIPKIVISNTMVPNAAATGPIPSRGLVLITTGLLVELEEDEILSVLGHEMGHLKGHDPVVLFSIISIEFILRFTLFFPLVILNPIIYIIVVMAAIFFVAKFFETRADLVSAMNIGQPEVLAESLRKIGFNRLQMERSSSNILRWLAWDPHPPLYFRIERLDKMKTPIKVKHPLIQSAKDVINGFKRSFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC86</accession>
    <name>F0TC86_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_2134"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OMA" id="WVECGEK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020075">
        <property type="entry name" value="Uncharacterised_AF2234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10967">
        <property type="entry name" value="DUF2769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="10784" checksum="CEE636432BBD3B16" modified="2011-05-03" version="1">MMDKFEQLMNDVGKMSPGDQNKAIEEYKASCICPTCPTWNKCAEEANEKLFCVTGISASCITELKGCMCPTCPLAQSLDVGVERNTYCLKGSEMEQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC1</accession>
    <name>F0TCC1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AAA ATPase</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2173"/>
    <dbReference type="EnsemblBacteria" id="ADZ10388">
        <property type="protein sequence ID" value="ADZ10388"/>
        <property type="gene ID" value="Metbo_2173"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG06456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029703_1_0_2"/>
    <dbReference type="OMA" id="WIVPLER"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="179"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="531" mass="60060" checksum="50DF7B5D9D1EC697" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF6</accession>
    <name>F0TCF6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645774.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2209"/>
    <dbReference type="EnsemblBacteria" id="ADZ10423">
        <property type="protein sequence ID" value="ADZ10423"/>
        <property type="gene ID" value="Metbo_2209"/>
    </dbReference>
    <dbReference type="GeneID" id="10278675"/>
    <dbReference type="KEGG" id="mel:Metbo_2209"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_3_2"/>
    <dbReference type="OMA" id="TKTDICR"/>
    <dbReference type="OrthoDB" id="83765at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="14344" checksum="F68715124867A4DB" modified="2011-05-03" version="1">MEVKEAMNQGVITVNPESKPLDAFKKMYKEGVRRLFVLDADGDPKGVVSYSDLIGVLGSLRPTNSEMASLSISDIMSEDVMTIDSDNGIEDAANLMVRADVSGLLVLEKDKPVGVITRTDICRMVAAELLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP1</accession>
    <name>F0TCP1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645782.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2217"/>
    <dbReference type="EnsemblBacteria" id="ADZ10431">
        <property type="protein sequence ID" value="ADZ10431"/>
        <property type="gene ID" value="Metbo_2217"/>
    </dbReference>
    <dbReference type="GeneID" id="10278683"/>
    <dbReference type="KEGG" id="mel:Metbo_2217"/>
    <dbReference type="eggNOG" id="arCOG02821">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012007_0_0_2"/>
    <dbReference type="OMA" id="EAAFKYK"/>
    <dbReference type="OrthoDB" id="35859at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="3">
        <location>
            <begin position="118"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="2">
        <location>
            <begin position="360"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="508" mass="56552" checksum="FE1B75050AF0614C" modified="2011-05-03" version="1">MINSENRQKPVYGVVGICGVVGNLVSRMLMDKKFRVMGTDMSPAKGCTFLYTLEDYDFPKFLENHPESFFSDSDIIIPPPSLGEDSELFKLISEHGNDVMTVDDCLNQFKPDKPVLCVSGTNGKTTTTTLLKHICRTMDLKPTEHGFIKLQGNIDYIPPLQARLKGDVAVLETGTFGNTGDLEIMMKRCEPDCGVLTNINPDHLDKTHDFINYARIKGEFVDYFQKKKLIINSDDPTVWGLIKNPDSVIKFGVDTTTEDVGYKECWCGNEIEINETITGSGYYQCECGLKRPEPDYLAFDVEESEGRFKLKTPDSVFEVKMKLIGLHNVYNVTGAIVAAREYFNLPMDKILTAVETFEGVSGRIEHLFTHEEMEVILDYGHNPAGIETVLRELKKVYETITVVLTVSSESGEHGDVEIFDKVLKFGDFIVPASHASRVVAETKVEEIESGKIVLTSESVVKFDKTGTLGASSDQVLDGMKTGLKCKSSALICLGEAAFKYKKSILELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCW1</accession>
    <name>F0TCW1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645852.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2287"/>
    <dbReference type="EnsemblBacteria" id="ADZ10501">
        <property type="protein sequence ID" value="ADZ10501"/>
        <property type="gene ID" value="Metbo_2287"/>
    </dbReference>
    <dbReference type="GeneID" id="10278753"/>
    <dbReference type="KEGG" id="mel:Metbo_2287"/>
    <dbReference type="eggNOG" id="arCOG02736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_3_2_2"/>
    <dbReference type="OMA" id="CEVAICE"/>
    <dbReference type="OrthoDB" id="99855at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="111" mass="12814" checksum="B00BBBE39D2F4D13" modified="2011-05-03" version="1">MRVAVAVSDDEKFTEHFGRAQKFLIYEFDGDKVELIDRRESKKVPGEKHQWSKSLNIVEDSDVVICHQIGMTAKPGLKSEGKKVVEDEGPVEEVLNRFVEHEKFLRKPLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T7</accession>
    <name>F0T5T7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644681.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1085"/>
    <dbReference type="EnsemblBacteria" id="ADZ09330">
        <property type="protein sequence ID" value="ADZ09330"/>
        <property type="gene ID" value="Metbo_1085"/>
    </dbReference>
    <dbReference type="GeneID" id="10277534"/>
    <dbReference type="KEGG" id="mel:Metbo_1085"/>
    <dbReference type="eggNOG" id="arCOG00461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="OMA" id="GPQGWWP"/>
    <dbReference type="OrthoDB" id="58645at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="69"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="238" mass="27301" checksum="2C46A8A5E4A02374" modified="2011-05-03" version="1">MNKYDNQEFIAIYTQLYDLYGPQGWWPLQDIVSGLTPTKSGAQTGYHPLDYKLPKTPNQRFEIILGTILTQNTAWTSAEKALKNLKNLDVIDPKKILSIDTETLKAAIKCAGFLNQKANYIIGIAEFFNELDGEIPTRKELLSVKGVGNETADSILLYAYKQPFFVVDAYTKRIFTQLKLVNEKDSYLEIKKKFESNLPQDYKLFNEYHALIVEHAKRYYSKKPYAVNDPLVDNKIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X3</accession>
    <name>F0T5X3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09366">
        <property type="protein sequence ID" value="ADZ09366"/>
        <property type="gene ID" value="Metbo_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1121"/>
    <dbReference type="eggNOG" id="arCOG07652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352117_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="24834" checksum="F45BA6595A45B939" modified="2011-05-03" version="1">MGRNIIIIMENVIQIMEEINKGKNIIFYNKNIYDCYDQLQDDYVCIYFNEPTPVKGKLIKIINEITEDDRPSLNRKTIAALKEILIKGLKYERLIICFNHFDSLTNKSVKVYQSLNSLENIQFVCSFNKKFKKEIYPFYSQFELANKEEYEKKTVKDEINMTFPLYLFIALLCFVIYLKAASSLYMAFILIGAAWFSLIIFRTLIFIGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T604</accession>
    <name>F0T604_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide TPR_2 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2297"/>
    <dbReference type="EnsemblBacteria" id="ADZ10511">
        <property type="protein sequence ID" value="ADZ10511"/>
        <property type="gene ID" value="Metbo_2297"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2297"/>
    <dbReference type="eggNOG" id="arCOG00398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_2_5_2"/>
    <dbReference type="OMA" id="CYWNLVS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="ASCH" evidence="3">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="43654" checksum="547E156D6B7F42B5" modified="2011-05-03" version="1">MSYIPILLFGSRHVELITGQKTATIRKLWKKPLIPGDRLHCYWNLVSKERKKLFEAVVTDVEVVKFGDLIKNDSLAKEEGYSTAKELETDFRKIYPEDTEDSSLFQVIRFRKLPIEEWEGKKIDEKSLITKRADILFDVGKYNKSVACYSAALKFDPDDVYLLNKKGDNLSRLGRFKEAVDCYNKAIELDSNNEYLYNNKAIALLNSNQPQKALKCNDKAMKINDSNEIILYWRGFILEMLGELDSALKCYNKILVINPEDPDVWNAKGNILSELERTEEALDCYDKALELCLDLSPDAAMWNRKGNALMELGRYAEALKCYNEAIKHDNGNDAFICNKGVALLELNRYEKAIECFRRALVINPSNEDAKILKDECLENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T607</accession>
    <name>F0T607_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2300"/>
    <dbReference type="EnsemblBacteria" id="ADZ10514">
        <property type="protein sequence ID" value="ADZ10514"/>
        <property type="gene ID" value="Metbo_2300"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2300"/>
    <dbReference type="eggNOG" id="arCOG01682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_5_2"/>
    <dbReference type="OMA" id="YIYYTFN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="13119" checksum="A0251DC27883CE10" modified="2011-05-03" version="1">MMLRIEPDDEDRSEELEELFKALGNVNRLILIYCLASTEKKSFSVTEMAEMMGLTQPAASQHLKILKTVNILHSNKQGNYIYYTFNKNALVEHKEKIDSLFCCVFNKCEHNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T640</accession>
    <name>F0T640_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysylphosphatidylglycerol synthetase/UPF0104</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_2334</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645898.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2334"/>
    <dbReference type="EnsemblBacteria" id="ADZ10547">
        <property type="protein sequence ID" value="ADZ10547"/>
        <property type="gene ID" value="Metbo_2334"/>
    </dbReference>
    <dbReference type="GeneID" id="10278800"/>
    <dbReference type="KEGG" id="mel:Metbo_2334"/>
    <dbReference type="eggNOG" id="arCOG00899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048072_1_1_2"/>
    <dbReference type="OMA" id="WRMLLEN"/>
    <dbReference type="OrthoDB" id="36454at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="353" mass="39133" checksum="7136313C5FD33157" modified="2011-05-03" version="1">MQTTSEFIHEHKWGILIAIIIGILIIFIVSVAVGLQDIINVLKDANLQIIAIAMLLEIVLICMWTERWSLILKVIDKSPGFSKLFVMMFASLFGNNITPGAAGGEPMRAYLLSKFENISFDLAFVSASADRVFEFFPFVLVSAFAIYMISTWHIGFWSTIFISLLIIVTMAFFGLLIYVGVKREIAERIILSIARSIFPYALKITKKETTFSAVSEQIQYYVERFSTGFATVLQDHKMFVLGLSISFLMWGTDMLRMYLCFAAVGSSPPIIPMIIIYTIAILITILPTIPGALGLREVTMVGLFLVVGVPADVVIAASLIDRVVSYLMPTGVGIIATAYYGKLLAKKDRSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C1</accession>
    <name>F0T6C1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1192"/>
    <dbReference type="EnsemblBacteria" id="ADZ09436">
        <property type="protein sequence ID" value="ADZ09436"/>
        <property type="gene ID" value="Metbo_1192"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1192"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054151_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="241"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="35763" checksum="B0548F792675B68F" modified="2011-05-03" version="1" precursor="true">MFFSVLLLIGLAVVLNVNFSSATTVNQTDIINSTHTINNSTVSHTVTSKASNKTVKTNSVSNSTKNMAAGGSTTVNGLSVSQLKDGISRVQIFYFKNSRMPNFVNFGTRKILIGTFQKNIATAGLSINLSVNGLTVAQLKDGISRVQSFYSNNGRLPNYVSYGTRKVTITTFQSNIATAGLKLSLSSGTIPIDTSSVSALAKSLAYGSSSQYETAQKIFNWVRDNVTYSFYYNTVYGASGTLTKRLGNCCDKTNLLVALSRAAGITAQYKWGSCYFTTSKTWYGHVWADLYLDGKWVAADTTSSRNSLGVINNWNTATFTLKGTYTTLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J1</accession>
    <name>F0T6J1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitrogenase</fullName>
            <ecNumber evidence="4">1.18.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645976.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2412"/>
    <dbReference type="EnsemblBacteria" id="ADZ10625">
        <property type="protein sequence ID" value="ADZ10625"/>
        <property type="gene ID" value="Metbo_2412"/>
    </dbReference>
    <dbReference type="GeneID" id="10278884"/>
    <dbReference type="KEGG" id="mel:Metbo_2412"/>
    <dbReference type="eggNOG" id="arCOG00594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_2_0_2"/>
    <dbReference type="OMA" id="VGFPTYD"/>
    <dbReference type="OrthoDB" id="15283at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="3">
        <location>
            <begin position="16"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="50676" checksum="4954E895C815BC80" modified="2011-05-03" version="1">MEHDKKFSVVNPSKMCQPMGAVQALLGVNDTMPIIHGSQGCSTYIRFQLTRHFREPIEVASTSMSEKTVIYGGEFNLMKALKNVSEKQSPKLIAVTSSCLTETIGEDMEGIIEKFKDANFDKNLPIIVPISTPSYVESHVEGYNRTIKALVENLAEPSTPNGKINIINGILSPADVKNVKQLLNDLHCPNIMLTDTSENLDAPLSEEALGLYKDGTTIEEIQDTANSLGTISISKHADSAAKFLEQKYGVRSVSGPLPLGIENTDQFVNNVCELTGLEVPESVKKDRGRLLDAMVDAHSYNYHRKVAIFGDPDFVSGMTRFTSEMGMTPTVVCTGAKSNKFSEDIDLIATQKGIDPVVLAGNDLYDMHREIKDVGADILLGNSYGASIAEEEKIPLFRVGFPIFDRMGAQRISTLGYNGGTEFVDRLTNTLLDFYYDEAGYELEKSEEIADEELFAEGELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K4</accession>
    <name>F0T6K4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645989.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2425"/>
    <dbReference type="EnsemblBacteria" id="ADZ10638">
        <property type="protein sequence ID" value="ADZ10638"/>
        <property type="gene ID" value="Metbo_2425"/>
    </dbReference>
    <dbReference type="GeneID" id="10278897"/>
    <dbReference type="KEGG" id="mel:Metbo_2425"/>
    <dbReference type="eggNOG" id="arCOG10256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929414_0_0_2"/>
    <dbReference type="OMA" id="LGGDYGW"/>
    <dbReference type="OrthoDB" id="64417at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="317" mass="34366" checksum="86A7FC5CB2B20344" modified="2011-05-03" version="1" precursor="true">MDLNNIYIISVAILVLIVGLASSNMVSAETFGESNDQQAWIQGTGSTLQFTYLQATISLTVHNNNDHAEFFKVSQQYTGDGTVTVTGTNSSAIDWVILSTNPSAIRMINSINPAGDLGWEIDAGQTKTVTFALKESNPPAPWYIQRGDSANTFWPTVNDPGLTSSWFLPNELEYLNPNLQVVKWQGTFFFYLKNMVASGPRVEGLVRAPIVPMNSVLTSSSPTVSYIDNENPSAQTAAWDVTLYPGQTKGYSYTYTYPNGTSVTPANKKTGSTQINDPSHGNASSTLPTKNTGVPWGLFLIGGIIIACGIIYARVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P7</accession>
    <name>F0T6P7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0027"/>
    <dbReference type="EnsemblBacteria" id="ADZ08280">
        <property type="protein sequence ID" value="ADZ08280"/>
        <property type="gene ID" value="Metbo_0027"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0027"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="OMA" id="DDYGAVY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11521" checksum="5AECAC9BC45242E7" modified="2011-05-03" version="1">MIFMKRVLWYLIAGSRGGTNRARIIEALHDRPYNINQLSLELDLDYKTIQHHIKVLEDHNILVNSGDEKKYGKMIFLSNRMEENYPIFIEILSKMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q1</accession>
    <name>F0T6Q1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643635.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0031"/>
    <dbReference type="EnsemblBacteria" id="ADZ08284">
        <property type="protein sequence ID" value="ADZ08284"/>
        <property type="gene ID" value="Metbo_0031"/>
    </dbReference>
    <dbReference type="GeneID" id="10276453"/>
    <dbReference type="KEGG" id="mel:Metbo_0031"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_8_5_2"/>
    <dbReference type="OMA" id="KFCWSTL"/>
    <dbReference type="OrthoDB" id="97752at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="12884" checksum="8F079A36AC56AC8D" modified="2011-05-03" version="1">MKIKYTTMIVKDMDESVSFYRDVIGFEVDSQHNPAPDIVITLLKGEGDTMIELIKDPQHDIGLYSIGMDVEDVEAIVEELISKGANITMDPVPITVGKLAFMEDPNGVKIALIQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R3</accession>
    <name>F0T6R3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">L-aspartate oxidase</fullName>
            <ecNumber evidence="5">1.4.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0043</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643647.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0043"/>
    <dbReference type="EnsemblBacteria" id="ADZ08296">
        <property type="protein sequence ID" value="ADZ08296"/>
        <property type="gene ID" value="Metbo_0043"/>
    </dbReference>
    <dbReference type="GeneID" id="10276466"/>
    <dbReference type="KEGG" id="mel:Metbo_0043"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_1_2"/>
    <dbReference type="OMA" id="PTAHHFM"/>
    <dbReference type="OrthoDB" id="8547at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="9"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="442"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="558" mass="61652" checksum="27F83BFCF2AEB33D" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U7</accession>
    <name>F0T6U7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644818.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09467">
        <property type="protein sequence ID" value="ADZ09467"/>
        <property type="gene ID" value="Metbo_1225"/>
    </dbReference>
    <dbReference type="GeneID" id="10277676"/>
    <dbReference type="KEGG" id="mel:Metbo_1225"/>
    <dbReference type="HOGENOM" id="CLU_2730408_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="8755" checksum="599DF39D66B9DB38" modified="2011-05-03" version="1">MTIWASKLVIFRNDDLNSTFEIRKNSIRNIQINKENRIIEIEYKDEDGWEEQIIPFESLRWFNYTMQDPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V4</accession>
    <name>F0T6V4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09474">
        <property type="protein sequence ID" value="ADZ09474"/>
        <property type="gene ID" value="Metbo_1232"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1232"/>
    <dbReference type="eggNOG" id="arCOG14004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3113115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="45" mass="5449" checksum="5A3478EDE1BB37D0" modified="2011-05-03" version="1">MNEKEIEEFVENFKGMLWDELDDELGEMSREDLITVIVKLKRRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y1</accession>
    <name>F0T6Y1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamate--cysteine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644852.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1259"/>
    <dbReference type="EnsemblBacteria" id="ADZ09501">
        <property type="protein sequence ID" value="ADZ09501"/>
        <property type="gene ID" value="Metbo_1259"/>
    </dbReference>
    <dbReference type="GeneID" id="10277710"/>
    <dbReference type="KEGG" id="mel:Metbo_1259"/>
    <dbReference type="eggNOG" id="arCOG10328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020728_3_0_2"/>
    <dbReference type="OMA" id="RYSWLLM"/>
    <dbReference type="OrthoDB" id="33807at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007370">
        <property type="entry name" value="Glu_cys_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006334">
        <property type="entry name" value="Glut_cys_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38761">
        <property type="entry name" value="PTHR38761"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04262">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_cys_ligase" evidence="4">
        <location>
            <begin position="12"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="525" mass="60685" checksum="1EDACA00A4F4A86B" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z0</accession>
    <name>F0T6Z0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cystathionine gamma-lyase</fullName>
            <ecNumber evidence="2">4.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644861.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1268"/>
    <dbReference type="EnsemblBacteria" id="ADZ09510">
        <property type="protein sequence ID" value="ADZ09510"/>
        <property type="gene ID" value="Metbo_1268"/>
    </dbReference>
    <dbReference type="GeneID" id="10277719"/>
    <dbReference type="KEGG" id="mel:Metbo_1268"/>
    <dbReference type="eggNOG" id="arCOG00060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_2"/>
    <dbReference type="OMA" id="THGGIIV"/>
    <dbReference type="OrthoDB" id="55005at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080146">
        <property type="term" value="F:L-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044540">
        <property type="term" value="F:L-cystine L-cysteine-lyase (deaminating)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="377" mass="41026" checksum="28A9E0A5FE7D44D2" modified="2011-05-03" version="1">MKFSTRSIHAGRKPDPITGAISPTICQTSTFTFDEFNKPGDYDYSRTGNPTRSALEEAIANLEGGNAGFCFSSGMAAVTTAIHLLKSGDHAVVGEDIYGGTHRLFSEIMTKYGIDFTFIRMDSEEKLEAAIKPNTKMIWIETPSNPLLNITDLSMVSKIAKEKQILTVADNTFPSPYFLRPIEYGIDLVLHSTTKYINGHSDVIGGAIVTTTEELAKDVHFLLNGMGTNAAPFDSWLILRGLKTLPLRMEKHASNAIAVAEYLLDHPKVSEVFYPGLESHPGHSIAKNQMDGYGGVVSFKLKTDVPKFIESLEIFHLAESLGGADSLVEHAATMSHASMSPDARKQAGISDDIIRLSIGLEDNDDLIEDLSKGLDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z9</accession>
    <name>F0T6Z9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644870.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1277"/>
    <dbReference type="EnsemblBacteria" id="ADZ09519">
        <property type="protein sequence ID" value="ADZ09519"/>
        <property type="gene ID" value="Metbo_1277"/>
    </dbReference>
    <dbReference type="GeneID" id="10277728"/>
    <dbReference type="KEGG" id="mel:Metbo_1277"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="OMA" id="QYGIYVH"/>
    <dbReference type="OrthoDB" id="100307at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="15965" checksum="3E8E00C5A3E9D8D3" modified="2011-05-03" version="1">MLNGLKDIIRKSSIHVCLVNTGGCNGCDIEVVALLSPRYDLEQYGIYVHNNPREADVLLITGALAEQWKDKLQKIYAKTPEPKVVVAIGNCPLTGDVFNQEGSSVNAPVSDYIPVDAEVSGCPPRPTEILAAILAVGPDAIAARGRQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C7</accession>
    <name>F0T7C7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps15</name>
        <name evidence="6" type="ordered locus">Metbo_0103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643707.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0103"/>
    <dbReference type="EnsemblBacteria" id="ADZ08356">
        <property type="protein sequence ID" value="ADZ08356"/>
        <property type="gene ID" value="Metbo_0103"/>
    </dbReference>
    <dbReference type="GeneID" id="10276528"/>
    <dbReference type="KEGG" id="mel:Metbo_0103"/>
    <dbReference type="eggNOG" id="arCOG04185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="OMA" id="EEHPKDM"/>
    <dbReference type="OrthoDB" id="98179at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_S15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_S13/S15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_S15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="PTHR11885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S13_N" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15368" checksum="E348A67CDFF342CF" modified="2011-05-03" version="1">MAAKPEWTEYSNEEIEELILKLRKEGKSTSVIGVILRDQYGIPDVKAVTGLKITAVLEKNGQTEEYPEELINLIKKAVNIRDHLEENPKDLHTKRGLRIIESKIRRLVRYYVREGVLPEGWRYDPKTAALLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D8</accession>
    <name>F0T7D8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-fuculose phosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-fuculose-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuculose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:12933"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57846"/>
            <dbReference type="EC" id="4.1.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.17" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643718.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0114"/>
    <dbReference type="EnsemblBacteria" id="ADZ08367">
        <property type="protein sequence ID" value="ADZ08367"/>
        <property type="gene ID" value="Metbo_0114"/>
    </dbReference>
    <dbReference type="GeneID" id="10276539"/>
    <dbReference type="KEGG" id="mel:Metbo_0114"/>
    <dbReference type="eggNOG" id="arCOG04226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_0_2"/>
    <dbReference type="OMA" id="ICRYGRS"/>
    <dbReference type="OrthoDB" id="101802at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="7">
        <location>
            <begin position="8"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="21336" checksum="D6C0C9704B75774A" modified="2011-05-03" version="1">MDHETVVKNMVETSHVLYNKGLVPGKSGNISVKFVKDGLNVVAVTPSGSSFKTLKETDIVLVDLEGNIVSNTNHVPTSELEMHLNIYKVRNDVGAVVHTHSPITTGFAFSDEKIRRFEGFGPIEEEFLKVVDYHPPGSSDLANTVSEGLLNQDVVVLKDHGMVAVGKNLDEATLLAEFVEDSAKIQFVKKMLMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E6</accession>
    <name>F0T7E6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inhibitor_I42 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643726.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08375">
        <property type="protein sequence ID" value="ADZ08375"/>
        <property type="gene ID" value="Metbo_0122"/>
    </dbReference>
    <dbReference type="GeneID" id="10276547"/>
    <dbReference type="KEGG" id="mel:Metbo_0122"/>
    <dbReference type="eggNOG" id="arCOG03545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032882_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036331">
        <property type="entry name" value="Chagasin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018990">
        <property type="entry name" value="Prot_inh_I42_chagasin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09394">
        <property type="entry name" value="Inhibitor_I42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141066">
        <property type="entry name" value="SSF141066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inhibitor_I42" evidence="1">
        <location>
            <begin position="40"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="13646" checksum="5554891B7955DA44" modified="2011-05-03" version="1" precursor="true">MKKLAILFATFVCFAMISGSFAATNQHTEKKVISPVQYHKFVVIVPNNPTTGSQWRSIFDQTKVKLVSKTYVPDKANLQGYVVGYGGYNVYTFSGELGQKITLKHVDASGKVFEKRNYWIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G2</accession>
    <name>F0T7G2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643742.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0138"/>
    <dbReference type="EnsemblBacteria" id="ADZ08391">
        <property type="protein sequence ID" value="ADZ08391"/>
        <property type="gene ID" value="Metbo_0138"/>
    </dbReference>
    <dbReference type="GeneID" id="10276564"/>
    <dbReference type="KEGG" id="mel:Metbo_0138"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113705_0_0_2"/>
    <dbReference type="OMA" id="NFEGECK"/>
    <dbReference type="OrthoDB" id="104203at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="11"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="15419" checksum="FEBB8E7A10BCC4A6" modified="2011-05-03" version="1">MELINEHEIGICKDTELEKDVKANFEGECAEVGMYLAMARLAQRDGLPEVAEVLKTIAFEEAAHAAVFAELNGVISSSLKENLEMMLKGETMANNEKKAAAKKAKVEDIDPAHDFFDESSRDEARHARMLKGILERYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G7</accession>
    <name>F0T7G7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S cluster domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643747.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0144"/>
    <dbReference type="EnsemblBacteria" id="ADZ08396">
        <property type="protein sequence ID" value="ADZ08396"/>
        <property type="gene ID" value="Metbo_0144"/>
    </dbReference>
    <dbReference type="GeneID" id="10276570"/>
    <dbReference type="KEGG" id="mel:Metbo_0144"/>
    <dbReference type="eggNOG" id="arCOG00297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127723_0_0_2"/>
    <dbReference type="OMA" id="INDTWEK"/>
    <dbReference type="OrthoDB" id="96786at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="129"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="201" mass="22435" checksum="E350481AFF07B7F2" modified="2011-05-03" version="1">MEHDGSTHDIFPEGELVKRVSIEEIRPCIVAENMIRSLLQLDSKLESVIPALANKYPPGKVNYIKEKKILTLNIADRLVTLYPSGKISMNKTVDELDAISVVRDIMGQINEAYLELNSYNPTDYSKVNEKLSGIGPLAIYNCLPKTDCERCGEATCMAFSIKLLAGDINLNQCQPLNEDGDVSCLNELLGREIMLMLGWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L7</accession>
    <name>F0T7L7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644936.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09585">
        <property type="protein sequence ID" value="ADZ09585"/>
        <property type="gene ID" value="Metbo_1345"/>
    </dbReference>
    <dbReference type="GeneID" id="10277796"/>
    <dbReference type="KEGG" id="mel:Metbo_1345"/>
    <dbReference type="HOGENOM" id="CLU_2534709_0_0_2"/>
    <dbReference type="OrthoDB" id="121498at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="83" mass="9592" checksum="E60EC2BB998500EC" modified="2011-05-03" version="1">MKKNDSRLGYRWTLEEDIKMCELRQEGYNFKEIGVMLNRSEKSCATRYSNRGFTLNPMKVLLNLAIIVISIISGYTLEPSILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P3</accession>
    <name>F0T7P3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1371"/>
    <dbReference type="EnsemblBacteria" id="ADZ09611">
        <property type="protein sequence ID" value="ADZ09611"/>
        <property type="gene ID" value="Metbo_1371"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1371"/>
    <dbReference type="eggNOG" id="arCOG10669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148448_0_0_2"/>
    <dbReference type="OMA" id="CENILII"/>
    <dbReference type="OrthoDB" id="128769at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="13858" checksum="7B6031A234F75235" modified="2011-05-03" version="1">MNNIIVLMGYDVTNGIIIITGYALLLLTSGVLVRLVLSKISHKEKKEIAPDNLWDTGAVIGKCENILIITFILSNEFTALAIIFAAKTIIRKDDIEKNSLFYLAGNMINVTYSIVFGVILKLITGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z2</accession>
    <name>F0T7Z2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0219 protein Metbo_1378</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. UPF0219 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644969.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1378"/>
    <dbReference type="EnsemblBacteria" id="ADZ09618">
        <property type="protein sequence ID" value="ADZ09618"/>
        <property type="gene ID" value="Metbo_1378"/>
    </dbReference>
    <dbReference type="GeneID" id="10277829"/>
    <dbReference type="KEGG" id="mel:Metbo_1378"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="OMA" id="YAKHKGK"/>
    <dbReference type="OrthoDB" id="12601at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="UPF0219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="FabH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG_CoA_synt_N" evidence="2">
        <location>
            <begin position="3"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="3">
        <location>
            <begin position="219"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="37263" checksum="FF4FFE3E5B97FE4F" modified="2011-05-03" version="1">MAGIVGYGVYIPSYRIKVEEIAKVWGDDPKAISRGLVVQEKSVPGPDEDTATISVEAARNALKRAKIDPQKIGAVYVGSESHPYAVKPTATIVSEAVEAAPELTAADLEFACKAGTAGMQICMGLVDSGMVEYGLAVGSDTAQGAPGDALEYTASAGGAAYVIGTENTLADFEGTYSFTTDTPDFYRREGKPYPKHGGRFTGEPAYFKHVLSGAKGLFDKMGTEASDYDHVIFHQPNGKFYIRVAKKLGFNEEQYKTGLLTPVIGNTYSGATPLGLAAVLDIAKPGDRIMAVSYGSGAGSDAFSITVNDRLEEVKEEAPFVTDMVSNKSYVDYAVYAKYKAKLKMAWTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T818</accession>
    <name>F0T818_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1404"/>
    <dbReference type="EnsemblBacteria" id="ADZ09644">
        <property type="protein sequence ID" value="ADZ09644"/>
        <property type="gene ID" value="Metbo_1404"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1404"/>
    <dbReference type="eggNOG" id="arCOG03432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026556_1_0_2"/>
    <dbReference type="OMA" id="NIWITHN"/>
    <dbReference type="OrthoDB" id="54190at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018702">
        <property type="entry name" value="DUF2207_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09972">
        <property type="entry name" value="DUF2207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="608" mass="68832" checksum="1BFD2B9EA28A10A6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T822</accession>
    <name>F0T822_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644999.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1409"/>
    <dbReference type="EnsemblBacteria" id="ADZ09648">
        <property type="protein sequence ID" value="ADZ09648"/>
        <property type="gene ID" value="Metbo_1409"/>
    </dbReference>
    <dbReference type="GeneID" id="10277860"/>
    <dbReference type="KEGG" id="mel:Metbo_1409"/>
    <dbReference type="eggNOG" id="arCOG02645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_9_2"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="183" mass="21178" checksum="6F2C9E1BA8E52AD6" modified="2011-05-03" version="1">MDAKDKKNRILDAALKLFNDKGIDNTSTSLISKEAGVATGTLYIYFETKVDLITELGKSIQEESLESFRDLIGSSAGHSSLKKYWLDKVEWGVNNPDKHKFMLQFKSSPYNKNIELKIPDHEEKLTNFIKNGIEAKVLKDLSPKYVSKFFSNHVTFTVEYIIHTNTRERERFFETFYDGIKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T892</accession>
    <name>F0T892_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formylmethanofuran--tetrahydromethanopterin formyltransferase</fullName>
            <shortName evidence="3">Ftr</shortName>
            <ecNumber evidence="3">2.3.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">H4MPT formyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ftr</name>
        <name evidence="6" type="ordered locus">Metbo_0252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible transfer of a formyl group from formylmethanofuran (formyl-MFR) to tetrahydromethanopterin (H(4)MPT) to produce 5-formyl tetrahydromethanopterin (5-formyl-H(4)MPT) and methanofuran (MFR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:18061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="2.3.1.101"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.101" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643855.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0252"/>
    <dbReference type="EnsemblBacteria" id="ADZ08504">
        <property type="protein sequence ID" value="ADZ08504"/>
        <property type="gene ID" value="Metbo_0252"/>
    </dbReference>
    <dbReference type="GeneID" id="10276684"/>
    <dbReference type="KEGG" id="mel:Metbo_0252"/>
    <dbReference type="eggNOG" id="arCOG02695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="OMA" id="VIMCPAE"/>
    <dbReference type="OrthoDB" id="62652at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00693"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00579">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03119">
        <property type="entry name" value="one_C_fhcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FTR" evidence="4">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="FTR_C" evidence="5">
        <location>
            <begin position="147"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="297" mass="31283" checksum="17935E20BA7DD4FC" modified="2011-05-03" version="1">MDINGVEIEDNFAEAFDIKVSRILITAATKKLALVAATEATGYGTSVIGCPAEAGIDCYVPPQETPDGRPGYVIMICNMNKDKLDHELLERVGMCILTAATTAVFDWLDEPDEKLKTGQKLKFFGDGYEQTIDVEGRKVHSIPLMSGDFCVEAELGIKAGVAGGNFFILAENQPAGLLAADAAVDAIEAVPGAITPFPGGIVASGSKVGSNKYKFLGASTNEKMCVTLKDEVEGCQIPEDVNGVYEIVIDGVDEDAVKAAMKAGIEAAVKVSGIIQISAGNFGGNLGKYQLQLHDVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T895</accession>
    <name>F0T895_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SigmaK-factor processing regulatory BofA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643858.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0255"/>
    <dbReference type="EnsemblBacteria" id="ADZ08507">
        <property type="protein sequence ID" value="ADZ08507"/>
        <property type="gene ID" value="Metbo_0255"/>
    </dbReference>
    <dbReference type="GeneID" id="10276687"/>
    <dbReference type="KEGG" id="mel:Metbo_0255"/>
    <dbReference type="eggNOG" id="arCOG11241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188102_0_0_2"/>
    <dbReference type="OMA" id="IITHLAM"/>
    <dbReference type="OrthoDB" id="131981at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010001">
        <property type="entry name" value="BofA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07441">
        <property type="entry name" value="BofA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9146" checksum="39AADC76B313DE14" modified="2011-05-03" version="1">MVLETVLFGIFIAVLLAIGLVILFKAAGIIIKILLHMIFGVVMLFIVNLLPFIDIPLNILTVLVAGFGGFIGVILLVILNLIGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F2</accession>
    <name>F0T8F2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine active site containing 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645054.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09703">
        <property type="protein sequence ID" value="ADZ09703"/>
        <property type="gene ID" value="Metbo_1467"/>
    </dbReference>
    <dbReference type="GeneID" id="10277918"/>
    <dbReference type="KEGG" id="mel:Metbo_1467"/>
    <dbReference type="HOGENOM" id="CLU_054347_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
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            <begin position="208"/>
            <end position="225"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="256"/>
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    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="362" mass="42231" checksum="9E560CAA44540EDF" modified="2011-05-03" version="1">MEVKKRLESTSKPAVYVWFGSIIFTVLFTILIWILGPNLDHFTATLIPINNLNHYYWKLPVRNFWTMAIAWGMYISNQLLIWGAIYWAQKNLTKEKINPTYNLTKYNVAVLSITVFFIFLHLIQTHIWFDGLAQDVPIITSQGSVILMLAIALVLLNPVQGLFLGKKAGKPFTSRVTEFIRHTHMYIISWALIYTFWFHPMATDPQLLTGFFFMFLLFTQMALAYTKVHFDLRWIVVLQSWVAIHALFVAFYNTIFFGNTDMWPMFFSGFAFMFVFTYMYALNVKRNVKLLVTAAYILLVAFIYIPAPYGFGRDPSFLFRLEFLWIPIILYGLAALIALTVFLWLKLKKDEKDVENLENTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G0</accession>
    <name>F0T8G0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1476"/>
    <dbReference type="EnsemblBacteria" id="ADZ09711">
        <property type="protein sequence ID" value="ADZ09711"/>
        <property type="gene ID" value="Metbo_1476"/>
    </dbReference>
    <dbReference type="GeneID" id="10277927"/>
    <dbReference type="KEGG" id="mel:Metbo_1476"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_2"/>
    <dbReference type="OMA" id="WGQELIE"/>
    <dbReference type="OrthoDB" id="77595at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="396" mass="42585" checksum="89643A6F34368F77" modified="2011-05-03" version="1">MDAIKILKDSFNIMHKELLEIDRGTLGGIIIMPIAFLLLFGFVFPGATTLSDMPVGVVDLDNGQGSNAFITELNTLNKNANNTMSLQNFTNVDTAKTQITQGKLDGTYIIPQGFSYNLTHGQQANVIFYVDNSNPQLSTQLESVASNSVNEINGIQATQNVMNTSTATNQTANAQAAKVTIFPYSTNVQTTIPGKTNNFNFLAPGLMMMIVMFSVMSALPEVISKESEKGTFDGLLSAPINQVSILLGKTAALGLRGLCQAAIILVLAVAIFGVTVNGSIILTFFMLVLGIFSFIGLGLIVVSLAKDQATGDTIIDLLVIPMMFLSGIFIPLQTLPWFLQDIAKIIPLTYAADALRKIMLLNANIGDVMSQTLILIGFGVVTMAIAIPIFRRSMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K3</accession>
    <name>F0T8K3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bile acid:sodium symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645105.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1524"/>
    <dbReference type="EnsemblBacteria" id="ADZ09754">
        <property type="protein sequence ID" value="ADZ09754"/>
        <property type="gene ID" value="Metbo_1524"/>
    </dbReference>
    <dbReference type="GeneID" id="10277975"/>
    <dbReference type="KEGG" id="mel:Metbo_1524"/>
    <dbReference type="eggNOG" id="arCOG02191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071291_0_0_2"/>
    <dbReference type="OMA" id="MAVCPSA"/>
    <dbReference type="OrthoDB" id="96924at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="PTHR10361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="154"/>
        </location>
    </feature>
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        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="285" mass="30704" checksum="DBA716F1E2CB9545" modified="2011-05-03" version="1">MEVILQQLASFSVLIYIVSTMLSMGLKFFPKQFFEPLKDKSLVLKSLAANFVVLPVVTYIILQLIPLQQGLAIGLVLMAAGAGSPFMLKLVQFMKADMAFAVGLMLILSTVTLIYMPLLLSLLIPGVSVNPLSIAGSLLGLIFVPLIVGTVLKLKYTKLAATIQPIFNRLSNIFIFMVVVLYLGLNYQDFITVFGTGALVAALMFVTAAFVVGYLLGGPSKNTRSVLGMGTAIRNSSAAFVVAVTNFSSEYDVMAMIIVVYMLSIILMFIISSSMGRGSKVLEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L2</accession>
    <name>F0T8L2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643905.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0302"/>
    <dbReference type="EnsemblBacteria" id="ADZ08554">
        <property type="protein sequence ID" value="ADZ08554"/>
        <property type="gene ID" value="Metbo_0302"/>
    </dbReference>
    <dbReference type="GeneID" id="10276734"/>
    <dbReference type="KEGG" id="mel:Metbo_0302"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124264_0_0_2"/>
    <dbReference type="OMA" id="ATWLEKN"/>
    <dbReference type="OrthoDB" id="71973at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="175" mass="18882" checksum="5FDF7B82EAC894EB" modified="2011-05-03" version="1" precursor="true">MKKYLIILFILLCAVVMVSGCVTDNKNNNETQTYSQNNVSFNYPGGWASANPTAPNAVAAVADPKSIQSGSPSTVVIIQKPNATASNLSAAYAANYATFFNNTGYQQVSEGNITVNGTGALENVYATNSTNPREYRAVWLNENGTIYVILCVANQSDFKNEQNNFNLIINSFSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N0</accession>
    <name>F0T8N0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Malate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="3">1.1.1.82</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.82" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643923.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0320"/>
    <dbReference type="EnsemblBacteria" id="ADZ08572">
        <property type="protein sequence ID" value="ADZ08572"/>
        <property type="gene ID" value="Metbo_0320"/>
    </dbReference>
    <dbReference type="GeneID" id="10276757"/>
    <dbReference type="KEGG" id="mel:Metbo_0320"/>
    <dbReference type="eggNOG" id="arCOG04874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040452_3_1_2"/>
    <dbReference type="OMA" id="TNTEPAM"/>
    <dbReference type="OrthoDB" id="32318at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046554">
        <property type="term" value="F:malate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="PTHR11091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="SSF89733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="343" mass="36611" checksum="5EA875E1FBE2F2AC" modified="2011-05-03" version="1">MNITAEQERSIITGILKCMKVSDEDASIVADVTVDADLKGFSSHGIGRFPQYIKGLKAGTIDPEAELSIENETDSMALINGNHKFGHVVTYTAMEIAINKAKKNGIGMVGVHDSNHFGVAGYYSDMAIMNDMIGMVIANTEPAVAPIGGKVPIIGTNPVAIGIPSNKNYVSVDMATSASARGKLLEAKRKGEKIPENVALDADGNPTIDPEEALNGSILPFGAHKGYALSFMIEILAGPLVRAAFGKQVTGTADPGVMCTKGDLVMAIDPSKFSDLEDFKEQVDDFVEEIRNSGNVFIPGDMEVKNIKDRKENGISIDETLMGQIKDISDELSFDLEKILSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U6</accession>
    <name>F0T8U6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="5">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1544</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + NAD(+) = 2-dehydropantoate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:61292"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:61294"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:16235"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645125.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1544"/>
    <dbReference type="EnsemblBacteria" id="ADZ09774">
        <property type="protein sequence ID" value="ADZ09774"/>
        <property type="gene ID" value="Metbo_1544"/>
    </dbReference>
    <dbReference type="GeneID" id="10278000"/>
    <dbReference type="KEGG" id="mel:Metbo_1544"/>
    <dbReference type="eggNOG" id="arCOG04139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_0_0_2"/>
    <dbReference type="OMA" id="WEKWVFL"/>
    <dbReference type="OrthoDB" id="57213at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApbA" evidence="10">
        <location>
            <begin position="9"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="ApbA_C" evidence="11">
        <location>
            <begin position="182"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="37813" checksum="02AA2705861C8B84" modified="2011-05-03" version="1">METVEFERIGIMGAGSLGIILGAYIAEKRQVDLIVSSKATADALTTNGARVVGKVNKTVPVHALRAKDMEGTYDLFLYMVKQTANGTAIPQMLDHSHDNTVVCTLQNGLPELAVSEAFSQDRTVGAPVSWGATAIEPGVSMLTTPINEAYFALGSMTPRGSKYLPNIQAILELMCTVHTSDNLLSLRWSKLVINTVMTGTSTAIGGSFAKVFDNDWALKSAAYVGRETIEVISASGTSMGIGELDFSKIFSFDSDETISKTMKNIYNLWNGSRQAEASMRQDLLKGRKCEVDSINGIVVDVGKKHEVLTPMNDLIVDIIHAKEAGTIPVNASCKALFEAKIAEIETASKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V6</accession>
    <name>F0T8V6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645135.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1557"/>
    <dbReference type="EnsemblBacteria" id="ADZ09784">
        <property type="protein sequence ID" value="ADZ09784"/>
        <property type="gene ID" value="Metbo_1557"/>
    </dbReference>
    <dbReference type="GeneID" id="10278013"/>
    <dbReference type="KEGG" id="mel:Metbo_1557"/>
    <dbReference type="eggNOG" id="arCOG04818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_268607_0_0_2"/>
    <dbReference type="OMA" id="VNYDTPW"/>
    <dbReference type="OrthoDB" id="145at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="256"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="636"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1048" mass="124241" checksum="844C1253CB6675E5" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0T931</accession>
    <name>F0T931_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08653">
        <property type="protein sequence ID" value="ADZ08653"/>
        <property type="gene ID" value="Metbo_0401"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0401"/>
    <dbReference type="eggNOG" id="arCOG13992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1607181_0_0_2"/>
    <dbReference type="OMA" id="IITAICM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="16278" checksum="6BB751028D39ECF4" modified="2011-05-03" version="1">MKYERYKPYHEKTADEDFRLNEKLALLSIVASNFMMTGHSPTLVMTKREEGDEVLLPVGGGQKDKAREIISRVDFRKLYRGGKGRKIDPLMIITAICMYVMRKDNPRRGDIRYSNDFIRNTGLTKEIYDHISGKLDVYGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T967</accession>
    <name>F0T967_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">V-type ATP synthase alpha chain</fullName>
            <ecNumber evidence="4 13">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">V-ATPase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpA</name>
        <name evidence="17" type="ordered locus">Metbo_0437</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AL-21</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="4 13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644040.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0437"/>
    <dbReference type="EnsemblBacteria" id="ADZ08689">
        <property type="protein sequence ID" value="ADZ08689"/>
        <property type="gene ID" value="Metbo_0437"/>
    </dbReference>
    <dbReference type="GeneID" id="10276874"/>
    <dbReference type="KEGG" id="mel:Metbo_0437"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="OMA" id="TAFVQVY"/>
    <dbReference type="OrthoDB" id="6736at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="PTHR43607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="15">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab_Xtn" evidence="16">
        <location>
            <begin position="84"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="14">
        <location>
            <begin position="212"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="232"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ADZ08689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="583" mass="64121" checksum="774EAC5D61DD7CC0" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T990</accession>
    <name>F0T990_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1614</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645192.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09841">
        <property type="protein sequence ID" value="ADZ09841"/>
        <property type="gene ID" value="Metbo_1614"/>
    </dbReference>
    <dbReference type="GeneID" id="10278071"/>
    <dbReference type="KEGG" id="mel:Metbo_1614"/>
    <dbReference type="eggNOG" id="arCOG06491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912357_0_0_2"/>
    <dbReference type="OMA" id="DELIECR"/>
    <dbReference type="OrthoDB" id="90662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14532" checksum="FE272CF9291AE902" modified="2011-05-03" version="1">MSGMGEKARTVVSGVGDKVSSSDEGSVVDSVGEKVSGLGEKIKGTVKSVPKSASKSTDDLSLRIDDYLDEKSSQLIVDWELATNNDIENIEKKYSKVAQDLSTLDTNFSEYRDETNDKIKKIEERLQKLEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C8</accession>
    <name>F0T9C8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645230.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1653"/>
    <dbReference type="EnsemblBacteria" id="ADZ09879">
        <property type="protein sequence ID" value="ADZ09879"/>
        <property type="gene ID" value="Metbo_1653"/>
    </dbReference>
    <dbReference type="GeneID" id="10278110"/>
    <dbReference type="KEGG" id="mel:Metbo_1653"/>
    <dbReference type="eggNOG" id="arCOG02797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193458_0_0_2"/>
    <dbReference type="OMA" id="DTTCRSG"/>
    <dbReference type="OrthoDB" id="112562at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="SSF69025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="79" mass="8261" checksum="0E00BD4F838E9870" modified="2011-05-03" version="1">MGVKEEIHAQIMGALAGAKFPLETPEELLGSFPDGAETTCRSGEVELKAGDAGKVLTGEDFPFKSAKEVADVIVERAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D6</accession>
    <name>F0T9D6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645238.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1661"/>
    <dbReference type="EnsemblBacteria" id="ADZ09887">
        <property type="protein sequence ID" value="ADZ09887"/>
        <property type="gene ID" value="Metbo_1661"/>
    </dbReference>
    <dbReference type="GeneID" id="10278118"/>
    <dbReference type="KEGG" id="mel:Metbo_1661"/>
    <dbReference type="eggNOG" id="arCOG00169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_2"/>
    <dbReference type="OMA" id="LIEFYRP"/>
    <dbReference type="OrthoDB" id="68860at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="56"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="245" mass="26461" checksum="6832704B089D1995" modified="2011-05-03" version="1">MRKTIISLVIPAIIIVIWAVLTTYTGIIPSYLIPSPHDVFEAFKSLLMNGTLIQDTIDTLSRVILGFIVAAIVAVPLGILIGWSETVENYLSFIIGILRPIPPIAWIPFAILWFGLGLGSAIFIIFVGSVFPILISTADGVKRIDKVYIESAYTLGADKLQTIKKVVFPAALPNIITGLKVGMGIALMCTVAAEMIGSNNGLGYLILTATSMLDSASAIVGMLAIGVIGLTFEYIFTRMERKLNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G0</accession>
    <name>F0T9G0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644059.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0456"/>
    <dbReference type="EnsemblBacteria" id="ADZ08708">
        <property type="protein sequence ID" value="ADZ08708"/>
        <property type="gene ID" value="Metbo_0456"/>
    </dbReference>
    <dbReference type="GeneID" id="10276894"/>
    <dbReference type="KEGG" id="mel:Metbo_0456"/>
    <dbReference type="eggNOG" id="arCOG05122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061369_1_0_2"/>
    <dbReference type="OMA" id="VRKAVNW"/>
    <dbReference type="OrthoDB" id="80238at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014825">
        <property type="entry name" value="DNA_alkylation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08713">
        <property type="entry name" value="DNA_alkylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="26151" checksum="FBCB9CE947AF0947" modified="2011-05-03" version="1">MELQEIVMRLELLSTPENIEGMEKFGITPEKTYAVRIPELRKIARESGKNHDLALELWELDFRETKILACMIDDPKMVSSDQLNAWVLEFDYWEICDQCCMNLFRKTSFAYDKIFEWGEHEAEFVKRAAFSLLAVMAVHDKKQPNQQFEQYYPLLISASTDNRNFVKKAVNWALRSIGKKNSSLNKSAIYLAEEILALNTKSSKWIASNALKELKSEKIRKKLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H3</accession>
    <name>F0T9H3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methylated-DNA--[protein]-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0469</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0469"/>
    <dbReference type="EnsemblBacteria" id="ADZ08721">
        <property type="protein sequence ID" value="ADZ08721"/>
        <property type="gene ID" value="Metbo_0469"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0469"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="OMA" id="EVASIPH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="8">
        <location>
            <begin position="102"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21429" checksum="57BB5746D9425CA0" modified="2011-05-03" version="1">MNKRKDLVIISIHKTNKLYFAAAFSQKGKIVRIALPQTNLEDLVTEISNYHPKFIINNMYDEIVGIIAEIYHGNTSNFDIELLELNISNESSHETATLNTTFERDVILEVASIPHGQVKTYKEIATAVKTQGYRAVGTAIGKNPFPIVVPCHRVVRSDGKIGGFRGGKQMKIEMLINEGFKIKSDKILLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J9</accession>
    <name>F0T9J9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ARMT1-like_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644098.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0495"/>
    <dbReference type="EnsemblBacteria" id="ADZ08747">
        <property type="protein sequence ID" value="ADZ08747"/>
        <property type="gene ID" value="Metbo_0495"/>
    </dbReference>
    <dbReference type="GeneID" id="10276935"/>
    <dbReference type="KEGG" id="mel:Metbo_0495"/>
    <dbReference type="eggNOG" id="arCOG04410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071520_1_0_2"/>
    <dbReference type="OMA" id="FLLKAKC"/>
    <dbReference type="OrthoDB" id="78551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="SSF111321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARMT1-like_dom" evidence="1">
        <location>
            <begin position="4"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="290" mass="32061" checksum="3C12BC1A2714F96C" modified="2011-05-03" version="1">MKVHYECAACFLRQSREALDLATDDEELKMDITEELVKIVSENFRRGGVSNVIGTQIHRTIKDKTGNNDPYRKEREISNDIACQYIPMVEKLIEDDSSLKNYLKVAIAGNVIDFGALGLDTDMESLIVTTMQKEPAVDDSTELETELETAKTVLYLADNIGEIVMDKMLIKKLADYDVDITVALKEEPILNDACMEDALKIGLNEVSKLTTTGTDSIGIISGDVSPEFMAKFMEADLIVAKGLGNYEGLGEMDLKDKPVFCLLNAKCKPVARDIGVEVGDNIVLKLNPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L6</accession>
    <name>F0T9L6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein MutS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mutS2</name>
        <name evidence="7" type="ordered locus">Metbo_0512</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Has ATPase and non-specific DNA-binding activities.</text>
    </comment>
    <comment type="cofactor">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00534"/>
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    </evidence>
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            <dbReference type="EMBL" id="ADZ08764.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">AL-21</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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            <end position="28"/>
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        <location>
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            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="100"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="127"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="435"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="499"/>
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    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="15"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S2</accession>
    <name>F0T9S2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
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    <organism evidence="3 4">
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09951">
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        <property type="gene ID" value="Metbo_1727"/>
    </dbReference>
    <dbReference type="GeneID" id="10278184"/>
    <dbReference type="KEGG" id="mel:Metbo_1727"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_8_1_2"/>
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    <dbReference type="OrthoDB" id="108205at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03385">
        <property type="entry name" value="PAP2_BcrC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR033879">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48317"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="56"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="21965" checksum="66180A5E7046C644" modified="2011-05-03" version="1">MIEQLNFAVFHLINQYAGMNQFLDTFMVLIAKYMPLLVVVGMIILWMFKGRKTRDIILYGFYAALIGLIVNYLIGFVYYHPRPFVIPVGTLLFSYPADSSFPSDHSTIIFSMALMLAYFKRTRFPGLLFLVLGIIGGTARVFAGVHFPLDIVGSLVVSGISVLLVLKLRTKLNPLNDFINVVYNKIPMVSSEVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S9</accession>
    <name>F0T9S9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645309.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09958">
        <property type="protein sequence ID" value="ADZ09958"/>
        <property type="gene ID" value="Metbo_1734"/>
    </dbReference>
    <dbReference type="GeneID" id="10278191"/>
    <dbReference type="KEGG" id="mel:Metbo_1734"/>
    <dbReference type="eggNOG" id="arCOG06479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099720_0_0_2"/>
    <dbReference type="OMA" id="PAILYEN"/>
    <dbReference type="OrthoDB" id="85787at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="234" mass="25964" checksum="1E5274EA064E28D4" modified="2011-05-03" version="1" precursor="true">MNTKTKALIVLGLIIIFTITLCTAYYLTPTSNEQKYAGVSTQNLGNITYGTVVKEGPYGNKESDTKIAIIMGVHPQESDAHNAMLNAIKQNSNSLKYSYTIYKVNVTQDPDDYQRGRMNGQLLANKFAVPDIINQKYNLTIDVHSNVGNWEKTRFIFAPVAGSSAESIGKNLSNELPWLSYYVPPNPTSTVYVTEPLINAGIPALVYETFHNDSDSEKYNHANEFLEAVDGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U1</accession>
    <name>F0T9U1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1747</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Removes the phosphate from trehalose 6-phosphate to produce free trehalose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645321.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1747"/>
    <dbReference type="EnsemblBacteria" id="ADZ09970">
        <property type="protein sequence ID" value="ADZ09970"/>
        <property type="gene ID" value="Metbo_1747"/>
    </dbReference>
    <dbReference type="GeneID" id="10278204"/>
    <dbReference type="KEGG" id="mel:Metbo_1747"/>
    <dbReference type="eggNOG" id="arCOG01216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037265_0_0_2"/>
    <dbReference type="OMA" id="FQPAKDF"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="PTHR43768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="271" mass="30765" checksum="FD85C0A4E09BA26F" modified="2011-05-03" version="1">MVKYLFNSLNDLKTFKEDQSVVITDIDGTISEITSEPSHAKITADMRNILFVLSSQFKFVGVLTGRDINDALNIIKLKKIVYMGNHGLQRLKNGKIITDSRVNVYVPIIKEIYEELTTKLKDSSGFNLEYKTLSLTVHYNECYPKCCAKKEVLNTISTMSLGKLVKIVEGRDLLEIRPPVGDDKGSVLQKFITENNIKKIIYIGDDINDVCAFKKLKELSKEQKILGVSVAVNSKEVPKHVKESANFYVENVQETFEFLKWLSDKDVNKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U7</accession>
    <name>F0T9U7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645327.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09976">
        <property type="protein sequence ID" value="ADZ09976"/>
        <property type="gene ID" value="Metbo_1753"/>
    </dbReference>
    <dbReference type="GeneID" id="10278210"/>
    <dbReference type="KEGG" id="mel:Metbo_1753"/>
    <dbReference type="HOGENOM" id="CLU_2550285_0_0_2"/>
    <dbReference type="OrthoDB" id="128519at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="82" mass="9829" checksum="91E6C9D7ACAC4FFC" modified="2011-05-03" version="1">MFDPKKLMDSAVDQVQKQATDMVTGNSNRRTGGSEIDELMYRMSRDMEDLRRKNQELTDRLNDFERQFNHLKDAINRNSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V0</accession>
    <name>F0T9V0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645330.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1756"/>
    <dbReference type="EnsemblBacteria" id="ADZ09979">
        <property type="protein sequence ID" value="ADZ09979"/>
        <property type="gene ID" value="Metbo_1756"/>
    </dbReference>
    <dbReference type="GeneID" id="10278213"/>
    <dbReference type="KEGG" id="mel:Metbo_1756"/>
    <dbReference type="eggNOG" id="arCOG01792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076542_1_0_2"/>
    <dbReference type="OMA" id="NFVFHEV"/>
    <dbReference type="OrthoDB" id="41337at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransf_11" evidence="2">
        <location>
            <begin position="93"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="29175" checksum="BD855F3B75D04BE0" modified="2011-05-03" version="1">MDEIEENLTKKEANYGNWVPSKYVYIPGLLGMLFVVVSYFYYPVIVLALIFLMVTMYTSYARAQFAPEGGDLQAKIQNLVLDELKWDGNGKLLDIGCGNAPLTIKALKKYPNAHATGIDYWGGMWEFSKEACETNAEIEGVTNRATFLKASASDLPFEDESFDAAMSNMVFHEVKDRADKKMVMKEALRVVKKGGVFSFQDLFEEKKIYGNIDDLLDTILDWGVEEVNFIHTSDYEFIPKSLKLPFMVGNIGIIHGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V1</accession>
    <name>F0T9V1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1757</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645331.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1757"/>
    <dbReference type="EnsemblBacteria" id="ADZ09980">
        <property type="protein sequence ID" value="ADZ09980"/>
        <property type="gene ID" value="Metbo_1757"/>
    </dbReference>
    <dbReference type="GeneID" id="10278214"/>
    <dbReference type="KEGG" id="mel:Metbo_1757"/>
    <dbReference type="eggNOG" id="arCOG14009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891446_0_0_2"/>
    <dbReference type="OMA" id="YGTINNW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="14768" checksum="0B4BC4C6EB7F1D05" modified="2011-05-03" version="1" precursor="true">MNKLILISLCTILMFLSPVFAADSGSGTNIQGFGNNTDIYGTINNWFSTIQGDVNGFIDTENLQAKFGNYVNTSNIESTVNSNLQNMDIQAYLNDLMNNTDVQSFINNLNIQSITNNPDIQSFLNSLKINTGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W4</accession>
    <name>F0T9W4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644138.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0535"/>
    <dbReference type="EnsemblBacteria" id="ADZ08787">
        <property type="protein sequence ID" value="ADZ08787"/>
        <property type="gene ID" value="Metbo_0535"/>
    </dbReference>
    <dbReference type="GeneID" id="10276980"/>
    <dbReference type="KEGG" id="mel:Metbo_0535"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_2"/>
    <dbReference type="OMA" id="SAKHPGQ"/>
    <dbReference type="OrthoDB" id="21317at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="PTHR43127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="62"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="287"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="364" mass="40642" checksum="BAF64BAC3B929447" modified="2011-05-03" version="1">MAIEDRIREIEEEIKKTPYNKATSHHVGKLKAKVSKLKEDALARSSSGTKGKGFHVKKSGDSTVVLLGFPSVGKSTILNQLTNAESKTGAYEFTTLDIVPGVMHYRGAKIQILDIPGIITGASKGKGRGREILSVARNADFILMVLDIFNPQHMKVILDELRNIGIRANELPPDVTVKQNKMGGINLINTVPLTNIDEKTIRSILNEYGIHSADVLIREDATIDRFVDSMDPSCIYVPMLVVINKIDLADEKYIEELKTKMPDAMFIAADKGINVDELKDEIFERLKLIRLYLKPQGKKADLEEPMIIRQGSTVGDVAAKLHRDFVRNFRHAKVWGSSVKFPGQKVGLEHVLKDKDILRLIIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X2</accession>
    <name>F0T9X2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L39e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl39e</name>
        <name evidence="5" type="ordered locus">Metbo_0543</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644146.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0543"/>
    <dbReference type="EnsemblBacteria" id="ADZ08795">
        <property type="protein sequence ID" value="ADZ08795"/>
        <property type="gene ID" value="Metbo_0543"/>
    </dbReference>
    <dbReference type="GeneID" id="10276988"/>
    <dbReference type="KEGG" id="mel:Metbo_0543"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="OMA" id="KMRYWRR"/>
    <dbReference type="OrthoDB" id="131584at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_L39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_L39e_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="SSF48662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="51" mass="6202" checksum="8876E47BB900576A" modified="2011-05-03" version="1">MSRNKPCAKKLRLSKATKQNRRVPLWVMLKTSRKVRTHPKMRQWRRSKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA01</accession>
    <name>F0TA01_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="4">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0573</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644175.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0573"/>
    <dbReference type="EnsemblBacteria" id="ADZ08824">
        <property type="protein sequence ID" value="ADZ08824"/>
        <property type="gene ID" value="Metbo_0573"/>
    </dbReference>
    <dbReference type="GeneID" id="10277018"/>
    <dbReference type="KEGG" id="mel:Metbo_0573"/>
    <dbReference type="eggNOG" id="arCOG00619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023342_1_1_2"/>
    <dbReference type="OMA" id="DRCNVCN"/>
    <dbReference type="OrthoDB" id="14505at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="503" mass="54539" checksum="0CE7D699045DB933" modified="2011-05-03" version="1">MPFKVERDHNLCKREFNRPGCCWYMCDNRDEELCKNCYSCFNNCPHDVYEIIDDEPYPLHHENCVGCRICEEMCPNNAIEVNAVPEDRRNVWTQSDIVEIGRKSETGSYKVRGCGATRVIPTFDDLVVIPAQVSRPPIDKYREPCNTKVTLGARFAENPLVIDTPIMIAAMSFGALSKEAKISLAMGATLAGTATNTGEGGMLPEERRYASKLIAQYASGRFGVSAKYLNNSEAVEIKIGQGAKSGMGGHLLGEKVTADVSRIRMIPEGTDALSPARHMDIVGPEDLSMKISQLREITDWKVPIIVKFTSGRVSDDVKIAAKAGADIIVVDGMQGGTGAGPDVVTEHSGVPTIAAIVEADEALKQINLRSKVNLVAGGGIRNGADVAKAIALGADAVYIATAALVSIGCRVCQMCFAGTCRKGIATQNPQLRRRLDYIEGGKRVARYIEAMTEEAVMLTQQAGNTDLLKLEKDDLRALTLESSTLTGVKMAGLEKPFKYKGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA07</accession>
    <name>F0TA07_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0579</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644181.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="SMR" id="F0TA07"/>
    <dbReference type="STRING" id="877455.Metbo_0579"/>
    <dbReference type="EnsemblBacteria" id="ADZ08830">
        <property type="protein sequence ID" value="ADZ08830"/>
        <property type="gene ID" value="Metbo_0579"/>
    </dbReference>
    <dbReference type="GeneID" id="10277024"/>
    <dbReference type="KEGG" id="mel:Metbo_0579"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_1_1_2"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="69007at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="7">
        <location>
            <begin position="148"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="315" mass="34300" checksum="71BEB492FD022F92" modified="2011-05-03" version="1">MKVSVIGASGRVGKAAAFCLAEENSVNNLVLISRKESFDKVEGEALDMYDALAAKDVNVKITASYDFEDVKNSDIVVLSAGQPRSSGMSRMDVAVPNAKIVAEYARAIAKFAPESVILVVTNPVDIMTHVALKASGFDKHKVFGLGNHLDSLRLKNLISQHFNIHVSEIHTRVMGEHGDNMVPIMSSTSIGGILLKYFKGYDDFDMDRTVEKVKNAGNYVIKKKGATEYGPAFAISNIAMIVLNDEKKILTVSALLDGEIEGVKDVCLGVPVKLGIDGIERIIPIIMTERERKAFLKAAEEVKTKTEEVMNAIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA12</accession>
    <name>F0TA12_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644186.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0584"/>
    <dbReference type="EnsemblBacteria" id="ADZ08835">
        <property type="protein sequence ID" value="ADZ08835"/>
        <property type="gene ID" value="Metbo_0584"/>
    </dbReference>
    <dbReference type="GeneID" id="10277029"/>
    <dbReference type="KEGG" id="mel:Metbo_0584"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104916_1_1_2"/>
    <dbReference type="OMA" id="IWNQVIT"/>
    <dbReference type="OrthoDB" id="75689at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="PTHR36536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00153">
        <property type="entry name" value="TIGR00153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="25283" checksum="07456EFABE484D2F" modified="2011-05-03" version="1">MKNLFKRESKVEEYSKDHVETVYRCVNQLNVLMKHFYENDFESIEKTAQEIADLEHEADIIRRKMEIEFFNGAFLPFDREDRIILAEEVDSVADMTEETGFGICLSKINFPTIYKTDFMELTESVQNTVSALKKCIELLSEDLGQALDTAHEVESLEDVVDKIERKILRRLYEDYKKGEIDILTHIELKSTVLRIGNIADRAENASDRVPIIVAKRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA33</accession>
    <name>F0TA33_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Potassium-transporting ATPase ATP-binding subunit</fullName>
            <ecNumber evidence="12">7.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP phosphohydrolase [potassium-transporting] B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Potassium-binding and translocating subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Potassium-translocating ATPase B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">kdpB</name>
        <name evidence="13" type="ordered locus">Metbo_1760</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit is responsible for energy coupling to the transport system and for the release of the potassium ions to the cytoplasm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + K(+)(out) = ADP + H(+) + K(+)(in) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.6" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645334.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1760"/>
    <dbReference type="EnsemblBacteria" id="ADZ09983">
        <property type="protein sequence ID" value="ADZ09983"/>
        <property type="gene ID" value="Metbo_1760"/>
    </dbReference>
    <dbReference type="GeneID" id="10278217"/>
    <dbReference type="KEGG" id="mel:Metbo_1760"/>
    <dbReference type="eggNOG" id="arCOG01577">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025728_2_0_2"/>
    <dbReference type="OMA" id="ILWLWFT"/>
    <dbReference type="OrthoDB" id="17967at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00285">
        <property type="entry name" value="KdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006391">
        <property type="entry name" value="P-type_ATPase_bsu_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43743">
        <property type="entry name" value="PTHR43743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01497">
        <property type="entry name" value="kdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="573"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="614"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="340"/>
        </location>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="344"/>
        </location>
        <ligand>
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        <location>
            <position position="517"/>
        </location>
        <ligand>
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            <position position="521"/>
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        <ligand>
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    <sequence length="682" mass="72587" checksum="B23729F740B12082" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA79</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Han C."/>
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                <person name="Mikhailova N."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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    <sequence length="80" mass="9405" checksum="CEA03B1114DE843C" modified="2011-05-03" version="1">MSPCRKTLKSFFKPKKEIFNHCLTKKMNSKLYKLNKAEKYEYPFKVKNLMGTSVLCFKVSNKLVSLFRIVSKVPTKKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Han C."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02829"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="319" mass="36340" checksum="C672E5EE4A021F1C" modified="2011-05-03" version="1">MRKPYVILIGSASGIGKSTIASELAKELGIKHLIETDFIRAIVRGIIGPDYAPSLHKSSFDAYLTLRDKERFDGDTDSLIEAGFEDHASFVIPAIERVIRRAVDDFDDVVIEGVHLVPGFIDIEKFQKDASIHFFVLTADKEVHKERFVKRAMKIKRGGKHLEYFKENRVINDYLVKSAIEHNIPVINNQTIDHTLKRMLTLIRGICKVMLFKHSVDQLEEETSIILDKYGGRMVDVSYFLPGFGEPLKRNVNVFDPVEAKRFIDKLDENPKRKKDLENLYSLSGNVHSHKICAPDKKSLEAMIKDLDDAGLIYKNEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH5</accession>
    <name>F0TAH5_METLA</name>
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        <submittedName>
            <fullName evidence="8">SSS sodium solute transporter superfamily</fullName>
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        <name evidence="8" type="ordered locus">Metbo_0676</name>
    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ08927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644278.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0676"/>
    <dbReference type="EnsemblBacteria" id="ADZ08927">
        <property type="protein sequence ID" value="ADZ08927"/>
        <property type="gene ID" value="Metbo_0676"/>
    </dbReference>
    <dbReference type="GeneID" id="10277121"/>
    <dbReference type="KEGG" id="mel:Metbo_0676"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_2_2"/>
    <dbReference type="OMA" id="GWWGMRR"/>
    <dbReference type="OrthoDB" id="8968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="527" mass="56786" checksum="9FFCB593712BB19F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH8</accession>
    <name>F0TAH8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">Metbo_0679</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644281.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0679"/>
    <dbReference type="EnsemblBacteria" id="ADZ08930">
        <property type="protein sequence ID" value="ADZ08930"/>
        <property type="gene ID" value="Metbo_0679"/>
    </dbReference>
    <dbReference type="GeneID" id="10277124"/>
    <dbReference type="KEGG" id="mel:Metbo_0679"/>
    <dbReference type="eggNOG" id="arCOG00029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="OMA" id="MKAYQRQ"/>
    <dbReference type="OrthoDB" id="117225at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="41"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="117"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19042" checksum="FC135EBDE1BDD8D9" modified="2011-05-03" version="1">MDKKNELITLLKENQVVKYGKFTLSSGRESSYYVDMKRAITDPIILNKIAELITELIADDKIDKVAGPALGAIPIVTAVSLESSIPMLMIRKSKKDYGTSELIEGDLEDEDIVVVVEDVTTTGNSLIKAIQAVSSNGGLVKRAFVVVDREEGAIENLKNEGIELEPLVTVKDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH9</accession>
    <name>F0TAH9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644282.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0680"/>
    <dbReference type="EnsemblBacteria" id="ADZ08931">
        <property type="protein sequence ID" value="ADZ08931"/>
        <property type="gene ID" value="Metbo_0680"/>
    </dbReference>
    <dbReference type="GeneID" id="10277125"/>
    <dbReference type="KEGG" id="mel:Metbo_0680"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="OrthoDB" id="108340at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19055" checksum="710029D1E91C0551" modified="2011-05-03" version="1">MKSKSMEEHKQSSPKSVNFAVITLSDSKYRDYLENKTTDISGNLIIDRLKNENEMVFYTVIPDDGDLLKATIEDIIEGTSAEIILTTGGTGIGSRDITIETLQPLFQKEITGFGEIFRQESYKEIGAGAILSRATAGVYKGIIIIAMPGSPNAVETGLKLIGSEVYHLVKHVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK8</accession>
    <name>F0TAK8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DGC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645431.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1859"/>
    <dbReference type="EnsemblBacteria" id="ADZ10080">
        <property type="protein sequence ID" value="ADZ10080"/>
        <property type="gene ID" value="Metbo_1859"/>
    </dbReference>
    <dbReference type="GeneID" id="10278317"/>
    <dbReference type="KEGG" id="mel:Metbo_1859"/>
    <dbReference type="eggNOG" id="arCOG03333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965592_0_0_2"/>
    <dbReference type="OMA" id="CMGATSA"/>
    <dbReference type="OrthoDB" id="116767at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014958">
        <property type="entry name" value="DGC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08859">
        <property type="entry name" value="DGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="13556" checksum="8D11836293A29556" modified="2011-05-03" version="1" precursor="true">MEKKKYALAPCSGMSPYGLITRAASSDVVRERDDLISICMGATSADREGFKELIKKYPIIAVNGCEGACVDKILLQKGVKVSKVVNAQEELNSANLKPTDPVRLDENGEKCVELLKQKITKLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ4</accession>
    <name>F0TAQ4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapA</name>
        <name evidence="10" type="ordered locus">Metbo_1906</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645477.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1906"/>
    <dbReference type="EnsemblBacteria" id="ADZ10126">
        <property type="protein sequence ID" value="ADZ10126"/>
        <property type="gene ID" value="Metbo_1906"/>
    </dbReference>
    <dbReference type="GeneID" id="10278364"/>
    <dbReference type="KEGG" id="mel:Metbo_1906"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_2"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="65776at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="296" mass="32155" checksum="D47CD104D88CBB1E" modified="2011-05-03" version="1">MSLKGTIVAMVTPFTKEDEVDEAGMRENINYLIDRGVDGLLVAGTTGESATITHDEQRKMIDILVDEVNGKVNAIAGAGSNSSKEALGLVQYAENAGADAALVITPYYNKPQQHGLVEHYKFLNEKTEIPKIVYNVPSRTGTDIEVETIVEVAKLDGIVAIKEANPDMDKVSATIKRLQEEQVEDFIVTSGNDNLTLPMIALGAQGVISVVANVDPARMSQMVNKALEGDYAGASKLHYELYDLMKVLFIESNPVPSKDSLNMMGRPAGHVRLPLAPMKDESTVKLTEVLKDLELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW4</accession>
    <name>F0TAW4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644341.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0739"/>
    <dbReference type="EnsemblBacteria" id="ADZ08990">
        <property type="protein sequence ID" value="ADZ08990"/>
        <property type="gene ID" value="Metbo_0739"/>
    </dbReference>
    <dbReference type="GeneID" id="10277186"/>
    <dbReference type="KEGG" id="mel:Metbo_0739"/>
    <dbReference type="eggNOG" id="arCOG04417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126374_0_0_2"/>
    <dbReference type="OMA" id="YVIDINF"/>
    <dbReference type="OrthoDB" id="120782at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018645">
        <property type="entry name" value="DUF2072"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09845">
        <property type="entry name" value="DUF2072"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="11606" checksum="1E609ECE081F66AA" modified="2011-05-03" version="1">MHRCIKCGTEFKINSEEIIIKGCPECGSKFFEYQKGDLKEIHEIKGDSIETIMIHEHGIYELNLPTLLEEDSIIVSDEEGTYVIDINFLLKKQMKERNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY5</accession>
    <name>F0TAY5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal peptide peptidase SppA, 36K type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645482.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1911"/>
    <dbReference type="EnsemblBacteria" id="ADZ10131">
        <property type="protein sequence ID" value="ADZ10131"/>
        <property type="gene ID" value="Metbo_1911"/>
    </dbReference>
    <dbReference type="GeneID" id="10278369"/>
    <dbReference type="KEGG" id="mel:Metbo_1911"/>
    <dbReference type="eggNOG" id="arCOG01311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_2"/>
    <dbReference type="OMA" id="CDELWAR"/>
    <dbReference type="OrthoDB" id="36138at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="3">
        <location>
            <begin position="110"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="312" mass="32453" checksum="756F44975118BFD3" modified="2011-05-03" version="1" precursor="true">MKKNSKIVLGILVGGSLLVIALLVATVSLLGGSNFLNSAPNGDTVAVIPLQGEIGYGSSGVSGETIVTPENVKDALNQAESDGTVSSILIKINSPGGSPVASEEIMNAINESKKPVVVWIGDTGASGAYLAASSADDIIASPSSMVGSIGVIMGLTDLSKYYENNGINKYSIKAGEYKDMGSDYRNLTTNETNMLQGMVNEDYAHFIDIVAVNRNLTVNYTQSIAEGKIYTGTQAKNLKLVNDTGGEKQALDAAAKLGGITGSYNTIDINPSSGLLDILNSMSSRIAYSIGLGIGNNLKNGTDNDLKLPSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB05</accession>
    <name>F0TB05_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="3 9">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cofH</name>
        <name evidence="11" type="ordered locus">Metbo_1931</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="3 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645502.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1931"/>
    <dbReference type="EnsemblBacteria" id="ADZ10151">
        <property type="protein sequence ID" value="ADZ10151"/>
        <property type="gene ID" value="Metbo_1931"/>
    </dbReference>
    <dbReference type="GeneID" id="10278389"/>
    <dbReference type="KEGG" id="mel:Metbo_1931"/>
    <dbReference type="eggNOG" id="arCOG00656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_2"/>
    <dbReference type="OMA" id="IYGHIEE"/>
    <dbReference type="OrthoDB" id="26917at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00293">
        <property type="entry name" value="((2_3_4_5-tetrahydroxypentyl)a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="58"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="374" mass="41967" checksum="B1BFAA99EB11722E" modified="2011-05-03" version="1">MFEQLNIDSYTEEILEKSLKTVISNEEALTLINSTGRELQALLFAADMRREELVGNNVTYVKNWNINFTNVCTGTCGFCAFKCDESSSEAYFLSNEEVVKRAKAAWNNGAKEVCIQGGLHPDVDAYYYQKLLIDIRKELPDIYIHAFSPMEILYGAQNSDMELSEELKMLKEAGLDSIPGNAAEILNDDVRKVLCPSKMNTAKWIEVVETAHKTGIPTTCTMMYGHIEELKDRVNHIDTLRQIQRRTGGFTEFVPLTFMHKNTPIFRKGISSPGSTGIEDLKVYAVARLMFGDLLQNIQVSWVKLGFKFAQVCLTAGANDLGGTLGEENISKSAGAQHGVRTNPETFQRIIENMGRVPAERDTLYRNVEPLKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB24</accession>
    <name>F0TB24_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylglucosamine 2-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.14" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645521.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1950"/>
    <dbReference type="EnsemblBacteria" id="ADZ10170">
        <property type="protein sequence ID" value="ADZ10170"/>
        <property type="gene ID" value="Metbo_1950"/>
    </dbReference>
    <dbReference type="GeneID" id="10278410"/>
    <dbReference type="KEGG" id="mel:Metbo_1950"/>
    <dbReference type="eggNOG" id="arCOG01392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041674_0_1_2"/>
    <dbReference type="OMA" id="RYNTERP"/>
    <dbReference type="OrthoDB" id="18594at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="1">
        <location>
            <begin position="22"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="435" mass="48914" checksum="2DBC588D700338CB" modified="2011-05-03" version="1">MKIAFIIGTRPEIIKMAPIIDLTQKRGIEYVLIHSGQHYDHEMSQKFFLDLEIEKPHYNIGVGSNSHGKQTAQMMEEIEKVLTQEKPDVVMVQGDTNAVLAGAVAASKLNLAVGHVEAGLRSFDRTMPEEINRKIADSCSTMYFVPTEESALNLAMESIDPHEIFITGNTVVDACIRHLKIAEENSNLLRELDVDGDILTLTMHRAENVDDPERLKNILDAIMELEDITVVFPVHPRTLKTLENFGYLEQLENSDHIKLTKPIGYLDFLLLLSKSKFVMTDSGGLQEEAITLNVPCITLRYNTERPETVTAGGNILVGSDKTKITETIKKISNDNELYKKMQEAKNPYGEGNSSEKILEALLKFEKEGKLDINSPENIMNGLSRSVLMIEDDVTVKEYETINPSTRVQLVFQGEKILFPSPELNLKHKQILLNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB54</accession>
    <name>F0TB54_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645551.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10200">
        <property type="protein sequence ID" value="ADZ10200"/>
        <property type="gene ID" value="Metbo_1980"/>
    </dbReference>
    <dbReference type="GeneID" id="10278440"/>
    <dbReference type="KEGG" id="mel:Metbo_1980"/>
    <dbReference type="eggNOG" id="arCOG00296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074768_0_0_2"/>
    <dbReference type="OMA" id="KMEVPCC"/>
    <dbReference type="OrthoDB" id="100045at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12837">
        <property type="entry name" value="Fer4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="30637" checksum="EECD9845E2F06351" modified="2011-05-03" version="1">MKRDVIRINEDKCTGCGDCIPGCPEGALQVVDGKARLISDLFCDGLGACIGTCPQGAIEIEQREAEEYDERKVMENIIKGGKNVIKAHLQHLNDHGQKKYLNQAIHLLEEKNIPVPNYEHEKTFACECPGAREMELEPSSEPSTEAVPDSKPELKNWPIQLQLLNPNAPYLKNADLLIAADCAPFAYPNFNQKFLKGKILIILCPKLDKTIEEYIDKLAEIFKIQDIHSISIVHMEVPCCSGIGVIVNRALEKAKKDITVEDYTISINGEVINTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB68</accession>
    <name>F0TB68_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644370.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0768"/>
    <dbReference type="EnsemblBacteria" id="ADZ09019">
        <property type="protein sequence ID" value="ADZ09019"/>
        <property type="gene ID" value="Metbo_0768"/>
    </dbReference>
    <dbReference type="GeneID" id="10277216"/>
    <dbReference type="KEGG" id="mel:Metbo_0768"/>
    <dbReference type="eggNOG" id="arCOG06478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133049_1_0_2"/>
    <dbReference type="OMA" id="GIMEIFE"/>
    <dbReference type="OrthoDB" id="141157at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="18201" checksum="624A8C1F252BE95C" modified="2011-05-03" version="1">MEEGRNLLLGVLGILLGLIVIIFPLISIFTVNTIAGIGVIFVGIWIMVKSLKNDSIAAGIAGLIVAIFAIMLGIVFIGDIKTFEFFSFIALYIVGFFIALAGVESLISGKGAKGKGTGVLGIIIGILFIVIGTFAGNPLVLAALIGAFLIIAGLVEILEPQLMEIPKETAKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB70</accession>
    <name>F0TB70_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644372.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0770"/>
    <dbReference type="EnsemblBacteria" id="ADZ09021">
        <property type="protein sequence ID" value="ADZ09021"/>
        <property type="gene ID" value="Metbo_0770"/>
    </dbReference>
    <dbReference type="GeneID" id="10277218"/>
    <dbReference type="KEGG" id="mel:Metbo_0770"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_4_1_2"/>
    <dbReference type="OMA" id="PWHFVVA"/>
    <dbReference type="OrthoDB" id="121018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="22353" checksum="AF15F98AC517C3FC" modified="2011-05-03" version="1">MEFNDLLMSRYATKKFDGKKIDEETLDKILEMIRFSPSALNVQPWKIKVVSDQETLDKLSSASMDQPQISSCSHLLVFCANPDMMGNAKKVVASMEEMGVPKENIEQFETVINTFIGMFNEEAAAVSEAQHNVFIAVTTALYAAKSVGVDSCPMQGFDPVSYTKILDIPAELVPTMIVPLGYPADEQMPKMRFSKEDIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC2</accession>
    <name>F0TBC2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dimethylallyltranstransferase</fullName>
            <ecNumber evidence="3">2.5.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644424.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0822"/>
    <dbReference type="EnsemblBacteria" id="ADZ09073">
        <property type="protein sequence ID" value="ADZ09073"/>
        <property type="gene ID" value="Metbo_0822"/>
    </dbReference>
    <dbReference type="GeneID" id="10277271"/>
    <dbReference type="KEGG" id="mel:Metbo_0822"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="OrthoDB" id="55053at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="35803" checksum="4426BB1A728DFB24" modified="2011-05-03" version="1">MEVVDILKKYSSDIDEEIQNSLSTVDPEELKKSTQHLIKAGGKKIRPSMVVLSAIAVGGAPEEALKTAAAVELIHTFSLIHDDIMDEDDMRRGEPSVHVLWGEPMAILAGDTLFSKAFETVLETPIDNVSYERVVGALKTVVDSCIKICEGQASDMCFEGNFEVSEEAYMNMIYKKTGALIAASTKSGAIIGGGTPEQVELLSEYGRLVGLAFQIQDDYLDVMSDEQDLGKPVGSDVVEGKMTLMVVNALSKASPEDKKELISILEAEDDTNVTRALELFNKYDSIDYAKNIALDNINTAKSLLDELPDSEAKESLSMIADFVVERIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC3</accession>
    <name>F0TBC3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="7">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gltX</name>
        <name evidence="11" type="ordered locus">Metbo_0823</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0823"/>
    <dbReference type="EnsemblBacteria" id="ADZ09074">
        <property type="protein sequence ID" value="ADZ09074"/>
        <property type="gene ID" value="Metbo_0823"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0823"/>
    <dbReference type="eggNOG" id="arCOG04302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OMA" id="MRFAPNP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="9">
        <location>
            <begin position="100"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1c_C" evidence="10">
        <location>
            <begin position="405"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="564" mass="64470" checksum="7B0971D0054FC113" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC4</accession>
    <name>F0TBC4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LL-diaminopimelate aminotransferase</fullName>
            <shortName evidence="2">DAP-AT</shortName>
            <shortName evidence="2">DAP-aminotransferase</shortName>
            <shortName evidence="2">LL-DAP-aminotransferase</shortName>
            <ecNumber evidence="2">2.6.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dapL</name>
        <name evidence="4" type="ordered locus">Metbo_0824</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23988"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="EC" id="2.6.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.83" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644426.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0824"/>
    <dbReference type="EnsemblBacteria" id="ADZ09075">
        <property type="protein sequence ID" value="ADZ09075"/>
        <property type="gene ID" value="Metbo_0824"/>
    </dbReference>
    <dbReference type="GeneID" id="10277273"/>
    <dbReference type="KEGG" id="mel:Metbo_0824"/>
    <dbReference type="eggNOG" id="arCOG01133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051433_0_0_2"/>
    <dbReference type="OMA" id="DFQARGC"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00466"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010285">
        <property type="term" value="F:L,L-diaminopimelate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033362">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate, diaminopimelate-aminotransferase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01642">
        <property type="entry name" value="DapL_aminotrans_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019942">
        <property type="entry name" value="DapL/ALD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43144">
        <property type="entry name" value="PTHR43144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03542">
        <property type="entry name" value="DAPAT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="36"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="109"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="411" mass="45911" checksum="6942442DDE344327" modified="2011-05-03" version="1">MSVKINENYLLLESSYLFAEISRRVEKFQTENPDAQVIKMGIGDVTKPLPKAVIKAFNEAVTEMGNSETFMGYGPEQGYSFLIDTIIENDYKSRGIELDIDEVFISDAAKCDTANIQEIFGLDNVIAVTDPVYPVYVDSNVMAGRTGSIQDSGRYEGVVYMPSTAENNFIPELPKSPVDLIYLCFPNNPTGTALKKEDLAKWVEYARENDAIILFDAAYEAYITEDDIPRSIYEIEGAKDVAIEFRSFSKNAGFTGTRCAFVVVPKQVKAVDSEGNKHDLNSLWNRRTTTKFNGVSYPVQKAAEAVYTDEAKKEIQENIDYYLENAKIIKNSMEELGLDVYGGVNSPYIWVKTPENMDSWSFFDLLLEEANVVGTPGVGFGPSGEGYLRLTAFNTLENTKEAMERISKLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE1</accession>
    <name>F0TBE1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645561.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1990"/>
    <dbReference type="EnsemblBacteria" id="ADZ10210">
        <property type="protein sequence ID" value="ADZ10210"/>
        <property type="gene ID" value="Metbo_1990"/>
    </dbReference>
    <dbReference type="GeneID" id="10278450"/>
    <dbReference type="KEGG" id="mel:Metbo_1990"/>
    <dbReference type="eggNOG" id="arCOG03597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170072_0_0_2"/>
    <dbReference type="OMA" id="NGSEMEQ"/>
    <dbReference type="OrthoDB" id="112624at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020075">
        <property type="entry name" value="Uncharacterised_AF2234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10967">
        <property type="entry name" value="DUF2769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10622" checksum="E3EA6725E2B8329E" modified="2011-05-03" version="1">MDKFEEALENVNSMAADERDKAIQNYKDSCICHTCASYTECSKDANEKLFCVTGKSNNCITELKGCECPACPLARSLDVGIVHNAYCLNGSEMEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG5</accession>
    <name>F0TBG5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645585.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10234">
        <property type="protein sequence ID" value="ADZ10234"/>
        <property type="gene ID" value="Metbo_2015"/>
    </dbReference>
    <dbReference type="GeneID" id="10278475"/>
    <dbReference type="KEGG" id="mel:Metbo_2015"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="GLRMLPW"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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                <person name="Brauer S.L."/>
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                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
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    <reference evidence="8" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
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    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
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    <comment type="function">
        <text evidence="5">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2077"/>
    <dbReference type="EnsemblBacteria" id="ADZ10296">
        <property type="protein sequence ID" value="ADZ10296"/>
        <property type="gene ID" value="Metbo_2077"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2077"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="OMA" id="YRQYNLD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="6">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="43873" checksum="1D82AADDA8A0B03D" modified="2011-05-03" version="1">MQFVHMADTHLGYRQYGLSERETDFLEVFDRAVEEVVSERPDFAIHSGDLFEYSRPPTRALLTAQQGIFKLKSANIPFYAIAGNHDIVMKKNALPPQILFKDFGLKVISPKNPYFIENGVFIGGAPYASKLTSKHLIERLNEVEKASEKYEKTILVLHQGIDRYLPYEYEVKIGDLPKTFKYCAFGHIHERIVDDFGDGKLVYPGSTEIWRSNEVDGYKKNGKGFYLVDMGGDVPEIEKINLEMSRDFIKEAVQYSKLEEEIKHLNDLITQMNKKPVLNLTVTGGNFSRSEIYETINNALADNCLSVRSRYKPDLVEEIMDLEKYEKDALNINRMIKERLKDFNDEKVSELATGLLKEMSEGDMDKASEMTRNFYEELHDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW5</accession>
    <name>F0TBW5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645658.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2088"/>
    <dbReference type="EnsemblBacteria" id="ADZ10307">
        <property type="protein sequence ID" value="ADZ10307"/>
        <property type="gene ID" value="Metbo_2088"/>
    </dbReference>
    <dbReference type="GeneID" id="10278550"/>
    <dbReference type="KEGG" id="mel:Metbo_2088"/>
    <dbReference type="eggNOG" id="arCOG03177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_6_2"/>
    <dbReference type="OrthoDB" id="116895at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16847" checksum="87121EA113EA2667" modified="2011-05-03" version="1">MHEILDDNSTVRLLTRSSNLLKKKLAVDLVDFDISPEQWAILTRVHGMEGSNQKNLAETSSKDRAAVTRILNILEDKGLVERKHSSYDKREFLIYLTSDGHKMYKETSEIISESLEEINSILDSAELKNMRQSLIKLISNLEDLTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC00</accession>
    <name>F0TC00_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645693.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2123"/>
    <dbReference type="EnsemblBacteria" id="ADZ10342">
        <property type="protein sequence ID" value="ADZ10342"/>
        <property type="gene ID" value="Metbo_2123"/>
    </dbReference>
    <dbReference type="GeneID" id="10278586"/>
    <dbReference type="KEGG" id="mel:Metbo_2123"/>
    <dbReference type="eggNOG" id="arCOG00304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106253_0_0_2"/>
    <dbReference type="OMA" id="GHNITNG"/>
    <dbReference type="OrthoDB" id="85944at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="23813" checksum="F849FAD792912253" modified="2011-05-03" version="1">MKKRIDLHTHSIFSDGELLPSEIARRALVLNHSAVAITDHVDASNIECVGNVIKAVTDIRDNWDIEIIPGAEITHAPAEIIDKLAHEARKNGAEIVVVHGETIVEPVIEGTNWSAVNCPEVDILAHPGLITVEEVEAAKENGIALEISSRRGHCLGNGHVAKLATEVGADLVVDTDTHMPGDLISYEMAHKVALGAGLKSKEIKKALEDNPKRILRSKGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC11</accession>
    <name>F0TC11_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
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    </evidence>
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    </evidence>
    <sequence length="373" mass="43724" checksum="E9B26B376CC1CFB4" modified="2011-05-03" version="1">MNTEDNFIMNLSDLKSFNYPKDKLFSLQINHAQVKYEFLVRLASNNKNLICFGSGAHAPDKMSPPIFRRLTWQDEFEESVIYYNDPTLYLPVKSPLRYDVKRLSLGWGVGTPDNWYLLTILDIIKILALKNRINPEDILFFGSSCGGFTSLILSTLMKKSQAIINNPQIFCKALEGHLENVLNVCFDKLDYDTALKEYGCRLDVFELFKHEKYIPNFTYVINVNSEIDFHRNLLPFLDRLNKSEYFHDQIQIILYSSENGHDGVLDKEKTVKLIKNHFNLQNECLTQNKNVIETYETTIKSLEDDLNDINMQLKIIAKTKSFRIAYLLHRIHNQFIKGNNKDKKEFLDWMYNKFTKKNSKQPTKYNPLNNLIK</sequence>
</entry>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
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    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
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    </feature>
    <evidence type="ECO:0000259" key="1">
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="727" mass="83823" checksum="569B331C9AF7BB3D" modified="2011-05-03" version="1"> </sequence>
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    <accession>F0TC21</accession>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="ADZ09172">
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        <property type="gene ID" value="Metbo_0923"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0923"/>
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        <property type="project" value="UniProtKB-KW"/>
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            <end position="26"/>
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    </feature>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="330" mass="37112" checksum="B5D49BC6A4950E06" modified="2011-05-03" version="1">MKRLYSKDLGIIFLLTALYIILISFPNFSGKYILDMVFFALFFLFIGYSLIALIRPEEDYKDILRKPVLLLEFSVLLTLTVSIILMFSYLGLKIRSLVMVLSVVIMVMSISAYIRRINHYNSLKYEKATGREGVDAVSQNIKTLTKSSVESAELPNETPVPKSESYPVENQGPNSEPDKLVVTEDNIREKFIKKVEEEEKPVKTSKSATAGKKVYLDLVIIDLLSIFVLITYFVKIQNIGVVSYFIGLLYMLFLVGYPVTRIFFAGKNSISKKSLIGISIGLSLPITSIIGTVLNSTPYGLSVHNILFPLAVLTLILSLYANKRVKSFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC27</accession>
    <name>F0TC27_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C1A papain</fullName>
        </submittedName>
    </protein>
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    </gene>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0931"/>
    <dbReference type="EnsemblBacteria" id="ADZ09178">
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        <property type="gene ID" value="Metbo_0931"/>
    </dbReference>
    <dbReference type="GeneID" id="10277380"/>
    <dbReference type="KEGG" id="mel:Metbo_0931"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424315_0_0_2"/>
    <dbReference type="OMA" id="HPMAIEY"/>
    <dbReference type="OrthoDB" id="3062at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040528">
        <property type="entry name" value="Lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18560">
        <property type="entry name" value="Lectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Pept_C1" evidence="2">
        <location>
            <begin position="75"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="645" mass="71201" checksum="0342773297B3CFF1" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC40</accession>
    <name>F0TC40_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644542.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09191">
        <property type="protein sequence ID" value="ADZ09191"/>
        <property type="gene ID" value="Metbo_0944"/>
    </dbReference>
    <dbReference type="GeneID" id="10277393"/>
    <dbReference type="KEGG" id="mel:Metbo_0944"/>
    <dbReference type="eggNOG" id="arCOG15223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_215167_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5202" checksum="1465F754EE279A37" modified="2011-05-03" version="1">MGEKTGEIKGKSKEIEGEVKGKVKEVEGEIKGKTKEVEGKIKGKLSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC57</accession>
    <name>F0TC57_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644559.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0961"/>
    <dbReference type="EnsemblBacteria" id="ADZ09208">
        <property type="protein sequence ID" value="ADZ09208"/>
        <property type="gene ID" value="Metbo_0961"/>
    </dbReference>
    <dbReference type="GeneID" id="10277410"/>
    <dbReference type="KEGG" id="mel:Metbo_0961"/>
    <dbReference type="HOGENOM" id="CLU_2217075_0_0_2"/>
    <dbReference type="OMA" id="MKQEAKG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11025" checksum="37A249CABF69F3FE" modified="2011-05-03" version="1">MKQEAKGKIFVGMIVSIVAFGFATGTGFFMGANPINTNGILNLTQQSQFPSIPASQTTNPTVKTNQTTAQSGQSSQTNTVTPSNPNSGNNSNKPTNNNQSGNSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC58</accession>
    <name>F0TC58_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644560.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0962"/>
    <dbReference type="EnsemblBacteria" id="ADZ09209">
        <property type="protein sequence ID" value="ADZ09209"/>
        <property type="gene ID" value="Metbo_0962"/>
    </dbReference>
    <dbReference type="GeneID" id="10277411"/>
    <dbReference type="KEGG" id="mel:Metbo_0962"/>
    <dbReference type="eggNOG" id="arCOG06455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_682593_0_0_2"/>
    <dbReference type="OMA" id="EGYREGG"/>
    <dbReference type="OrthoDB" id="36506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="45006" checksum="C2B767D0B34B027D" modified="2011-05-03" version="1">MVMDYISRTIFGFLIKHRVISIGTKYYPTNDMEREYVQMVNYTRTMLLEIETAHITTENIFNNVLSEVGTGNIPDNRKFIELHPAENNVNEYALLSNIIMGSDRYLYIEIFQKNKPLIEEFVRYIQRARGTIVERSATEIVSKMLSKNDAIRVGIELIAKGLDKEIDVRVAVGMTGAAAIERSINLNKQIGETSGVGFTKLGGEFAIVFSSKFGNLEGSPAVYDNYVFIDAIDSTKFIDENGRDKLVEIMNEISSFIEKECKGKIEGYRVGGDDLIANLPTKDAALRAGMDSAWHALNNGVRLRVGIGKSRREAGERAQMADNIKIWNNNPVMVFDLADGIYSYYIPSEFTRTVLDFLLNKKGRIAIIFIFVFVATLVGWNIGQPVFGLIAIGLALVYALTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC79</accession>
    <name>F0TC79_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="4">IMP dehydrogenase</shortName>
            <shortName evidence="4">IMPD</shortName>
            <shortName evidence="4">IMPDH</shortName>
            <ecNumber evidence="4">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">guaB</name>
        <name evidence="13" type="ordered locus">Metbo_0983</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 10">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="4"/>
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        <property type="protein sequence ID" value="ADZ09230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ09230">
        <property type="protein sequence ID" value="ADZ09230"/>
        <property type="gene ID" value="Metbo_0983"/>
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    <dbReference type="GeneID" id="10277432"/>
    <dbReference type="KEGG" id="mel:Metbo_0983"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_022552_2_1_2"/>
    <dbReference type="OMA" id="MGYCGAK"/>
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        <property type="reaction ID" value="UER00295"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="CBS_dom"/>
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        <property type="entry name" value="CBS_dom_sf"/>
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        <property type="entry name" value="PTHR11911"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
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    <keyword evidence="4" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
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    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="158"/>
            <end position="213"/>
        </location>
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            <begin position="337"/>
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            <begin position="360"/>
            <end position="361"/>
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        <location>
            <begin position="384"/>
            <end position="388"/>
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        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="4 5">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <begin position="297"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
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        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>K(+)</name>
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            <note>ligand shared between two tetrameric partners</note>
        </ligand>
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        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
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        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
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        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="495" mass="52801" checksum="B832B645EAC2118F" modified="2011-05-03" version="1">MFSEKLINAPEGFTFDDFLMLPSVSSVEPKDVETTTQISRNYRINIPIVSSAMDTVTEGEMAIALAQEGGLGVIHRNMTINEQINEIKKVKRSGDLTIRDVITISPEASIVEAQEIMDEEEISGLPVVRDGTVVGIISRRDVKPIINSDPKRMVQDIMTEEVVTVSESTTPAEALDIAYENKVERLPVVKDGIIVGILTMRDILERKNFPNASRDKKGRFLVAAATGPFDLDRAMALDEAGADIIAVDCAHAHKPSIVDSVKTMNENIDADLLVGNIATGEAAEDLMAAEVDGFKVGIGPGSICTTRIISGVGVPQLTAISSVADVAKDYGVPVIGDGGLRYSGDIAKAIGAGADAVMLGSLLAGTREAPGDVVIMNGRKFKQYRGMGSLGAMTGGVGAGTDRYFQEVKGPMKHAKLVPEGVEGVVPYKGPANEILFQLIGGIKASFGYCGANNIKDMQKKAKFVRISPSGMTESHPHDLTITNESPNYPTTRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC96</accession>
    <name>F0TC96_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645714.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2146"/>
    <dbReference type="EnsemblBacteria" id="ADZ10363">
        <property type="protein sequence ID" value="ADZ10363"/>
        <property type="gene ID" value="Metbo_2146"/>
    </dbReference>
    <dbReference type="GeneID" id="10278609"/>
    <dbReference type="KEGG" id="mel:Metbo_2146"/>
    <dbReference type="eggNOG" id="arCOG10208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930229_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="7192" checksum="5B9651ABAFAEB62C" modified="2011-05-03" version="1">MIKEKNEKKIKDDWKCPFSLKNDKKSSLKQIICKGCDTMFKSNYNSNYCLECKEIFYDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA1</accession>
    <name>F0TCA1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Type II site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="17">3.1.21.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Metbo_2153</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AL-21</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4" evidence="17"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645719.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2153"/>
    <dbReference type="REBASE" id="33854">
        <property type="entry name" value="Msp21ORF2153P"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADZ10368"/>
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        </location>
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            <dbReference type="EMBL" id="ADZ10368.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
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    <sequence length="1021" mass="118511" checksum="387EB2A121EF0A24" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA2</accession>
    <name>F0TCA2_METLA</name>
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        </submittedName>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="346" mass="40669" checksum="E70808378BE6B4E1" modified="2011-05-03" version="1">MWEDDFANLLFSNDPTRVNAKEAAELKYNKFPSHLYKYRTFDENEYSVKALEEDKIFLSNPLEFNDPYDCALLCKDANYVKEDFLKNLLAKDPKGFRKAHNLTKKQISKLQKSDKIIKDLSIFIAKNSPEYKNNPKRLNELSKENELQIRAISADFNELRKDMLVSCFSEDYKSILMWSHYAKEHTGFCVEYDFKSLGHNNHLTRNLFPVLYANEIFTIDDYIYNPQPQFPNILSSYLEGIDLNKIMDGIQFEILGNNKYNNMFPVCAALTKYEGWKYEKEWRYVLYSRNESKSTYINVPKPTAIYLGTKSNKKDIILEIANNKNIDVYQMQMKSSDYALEAEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB4</accession>
    <name>F0TCB4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2166</name>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2166"/>
    <dbReference type="EnsemblBacteria" id="ADZ10381">
        <property type="protein sequence ID" value="ADZ10381"/>
        <property type="gene ID" value="Metbo_2166"/>
    </dbReference>
    <dbReference type="GeneID" id="10278629"/>
    <dbReference type="KEGG" id="mel:Metbo_2166"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="HGTIFYR"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="24417" checksum="F18D8E28715FB3CA" modified="2011-05-03" version="1">MNNDNSSIRLVDVWKIYKNGDEVTHSLADLNHTFQRGSMSIIYGPSGSGKSTFVRIVGLLENITMGQIFVNGTDTSQMSQQDKNSIIQREIGFVFRNENLIPTLSAFENVNLPMNNSDSTPTQELLKTVGFNDFKKYPNDLSKEEEIRVSIARAMVNNHSVLITDEPTGDLHRSEAAEIMNLLQDLNKSLNLTIILTTNNKKLGAYGDFLELEDGKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC2</accession>
    <name>F0TCC2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10389">
        <property type="protein sequence ID" value="ADZ10389"/>
        <property type="gene ID" value="Metbo_2174"/>
    </dbReference>
    <dbReference type="GeneID" id="10278637"/>
    <dbReference type="KEGG" id="mel:Metbo_2174"/>
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    <dbReference type="HOGENOM" id="CLU_200893_1_0_2"/>
    <dbReference type="OMA" id="MILICNG"/>
    <dbReference type="OrthoDB" id="128226at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="6469" checksum="27543EF9BAB6301F" modified="2011-05-03" version="1">MANPSAILFSHIIPPIMAVISVILICAGVMDKKNVYTIIGIILFFAAGIIPFLVLPFLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG0</accession>
    <name>F0TCG0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0990"/>
    <dbReference type="EnsemblBacteria" id="ADZ09237">
        <property type="protein sequence ID" value="ADZ09237"/>
        <property type="gene ID" value="Metbo_0990"/>
    </dbReference>
    <dbReference type="GeneID" id="10277439"/>
    <dbReference type="KEGG" id="mel:Metbo_0990"/>
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    <dbReference type="OrthoDB" id="11161at2157"/>
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    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="50"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="386" mass="44424" checksum="44353B9954ABF55E" modified="2011-05-03" version="1">MKFIRDSLHGNLQINEFEVKLIDTPQIQRLRRIKQLGFTYLVYPGANHTRFEHSIGTMYLASRLSYGLKLPDEQRQILRVCALLHDAGHGPFSHVSEAVLKQSHEELTSKLIRESEISTILSEKYDPEEIISLIRGEGSLGQIISGDLDVDRMDYLLRDSYYTGVAYGVIDVERLIHNMKLEDNLILKSKGVQAAESMLLARYFMYPSVYQHHTTRIINSMFRRCLKQLFKEGHIDPENIYKYDDADIISIARYQEGLIGDIIHRLDNRKLFKTVYSLKLDELTNPGAIFKIEPKQIQQYEIQIAEELGVPEDYVIVDVPDYPSFDEMKTLVSSNGDLVNLSKISTIVSTLRDARFNHADLCVYLPEEYSNLAKDVSFNQLIDNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG7</accession>
    <name>F0TCG7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0997"/>
    <dbReference type="EnsemblBacteria" id="ADZ09244">
        <property type="protein sequence ID" value="ADZ09244"/>
        <property type="gene ID" value="Metbo_0997"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_2353233_0_0_2"/>
    <dbReference type="OMA" id="VETVWAN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10937" checksum="840EC3AE4D93637F" modified="2011-05-03" version="1">MVKGKIADEDINAFEEAYDSIKEEPAPEGLVASYLLKDMIDPNIYKVETVWANPETLKKIMGEGSASADIKLFEKFDTDPTVEIYEVRNNFPQYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ6</accession>
    <name>F0TCJ6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-oxalocrotonate tautomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644624.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1026"/>
    <dbReference type="EnsemblBacteria" id="ADZ09273">
        <property type="protein sequence ID" value="ADZ09273"/>
        <property type="gene ID" value="Metbo_1026"/>
    </dbReference>
    <dbReference type="GeneID" id="10277475"/>
    <dbReference type="KEGG" id="mel:Metbo_1026"/>
    <dbReference type="eggNOG" id="arCOG02240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_2_1_2"/>
    <dbReference type="OMA" id="PIITLEM"/>
    <dbReference type="OrthoDB" id="124315at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="6662" checksum="0783C6E333D47A4F" modified="2011-05-03" version="1">MPVITIDGPKMSKEQKAELVKEYSETASRIMKLPVEAMVIIIREVEGENVGTGNKLLCDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP5</accession>
    <name>F0TCP5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645786.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2221"/>
    <dbReference type="EnsemblBacteria" id="ADZ10435">
        <property type="protein sequence ID" value="ADZ10435"/>
        <property type="gene ID" value="Metbo_2221"/>
    </dbReference>
    <dbReference type="GeneID" id="10278687"/>
    <dbReference type="KEGG" id="mel:Metbo_2221"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="OMA" id="VQCEMCG"/>
    <dbReference type="OrthoDB" id="105095at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="CHP00270"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00270">
        <property type="entry name" value="TIGR00270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="158" mass="17845" checksum="C7F0A58DD85DAE58" modified="2011-05-03" version="1">MRCEICGKKLVEEPLKTKIDGSIMLTCKECSKFGKVQKEPPKPKRRGAPRGSSGTRRTSRPQEPTEEVIENFNQLVRNAREKKGWSREELGEKLYEKASVISRIESGKMVPDIKLAKKLEKTLKVVLIEKNDGTAMDELAHAKVREATIGDIARIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCW3</accession>
    <name>F0TCW3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S3Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps3ae</name>
        <name evidence="4" type="ordered locus">Metbo_2289</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645854.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2289"/>
    <dbReference type="EnsemblBacteria" id="ADZ10503">
        <property type="protein sequence ID" value="ADZ10503"/>
        <property type="gene ID" value="Metbo_2289"/>
    </dbReference>
    <dbReference type="GeneID" id="10278755"/>
    <dbReference type="KEGG" id="mel:Metbo_2289"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OMA" id="GQNAYTK"/>
    <dbReference type="OrthoDB" id="96138at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="30S_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_S3Ae"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="194" mass="22582" checksum="D4ABC06BF8589546" modified="2011-05-03" version="1">MAKARRRRVRDTWKEKQWYTIMTPKDFGDQEIGTTPAREPEQLMKRRVESSLRELTGDFSKQYIKLFFEINEVAGDTATTSFVGHQVTTDYVRSMIRRGTSRIDSIVNVTSQDGVKLKVHVLAITIKRAKSSQQKFIRETMEKMVQNAAKDKKFQELIEDILGGKMASHIYHESKKIYPLKRVETIKTRVIVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S2</accession>
    <name>F0T5S2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644666.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1069"/>
    <dbReference type="EnsemblBacteria" id="ADZ09315">
        <property type="protein sequence ID" value="ADZ09315"/>
        <property type="gene ID" value="Metbo_1069"/>
    </dbReference>
    <dbReference type="GeneID" id="10277518"/>
    <dbReference type="KEGG" id="mel:Metbo_1069"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="GNKKGWL"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="15"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="469" mass="51773" checksum="03B3335DAC44E520" modified="2011-05-03" version="1">MDTSHNNNKVNKNYILLIVIIASFLTPFIGSSLNIALPTIANELSANAILLSWITTSFFLTSAMFALPMGRIADIYGMKKVFRYGIIILTLACFLSAIAPSVDFLIVSRVIQGIGSAMIFVTGLAIIISVFPSSERGKAIGINVTTVYLGLVIGPVLGGFLTQYFGWRSIFYLMIFMGFMLTILIFWKMNEVEDGERGSEKIDYPGSLLYMLMLALILIGFSNIKGTFGKFMIMLGILSLISFIIWELRVENPVLEIKMFMGNKPFVLSNFTIILNYMGILSIGFLLSLYLQYIKGFNPNITGLILAVQTVVMVLTSPVAGKLSDRFDSGRLALFGTILITIGLFIFTLLNMETSVYLLIVGLIFVGIGIGLFSAPNTHLIMSSVEKRYFGLASASASTMRLLGQTFGMGFTLIIFEVYLGKIQFNPQNYPELLISTRVAFLIFTILGVAAILISLLRNILNKINKQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V4</accession>
    <name>F0T5V4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1102</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644698.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1102"/>
    <dbReference type="EnsemblBacteria" id="ADZ09347">
        <property type="protein sequence ID" value="ADZ09347"/>
        <property type="gene ID" value="Metbo_1102"/>
    </dbReference>
    <dbReference type="GeneID" id="10277552"/>
    <dbReference type="KEGG" id="mel:Metbo_1102"/>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_1_2"/>
    <dbReference type="OMA" id="QLVHMLV"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="77"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="291" mass="33143" checksum="8F7C8165F0794BCF" modified="2011-05-03" version="1">MISGFLIAIPSLDPIYNILGRGDYFILLLLLLVILMVALLTERVEKVRTLNKLNQELKTEAMKLEDANNELEAFAYSVSHDLRVPLRAIDGFSRIVIEDYEKELDEEGVRLLNVIRDNTKKMGQLIDDILLLSRAGRQEMNNSKLDMTSLAKNVYNEFQQDVSSRNIDFVVEDMPPVDADRALMTQVLTNLIGNAIKFTKKTENPRIELGYTEDEANYIYHVKDNGAGFNMKYYDKLFGLFQRLHSQEEFEGTGVGLSIVQRIISRHNGRVWGEGEVGKGATIYFSLPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V6</accession>
    <name>F0T5V6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13 14">UvrABC system protein B</fullName>
            <shortName evidence="13">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrB</name>
        <name evidence="19" type="ordered locus">Metbo_1104</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AL-21</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13 14">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644700.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1104"/>
    <dbReference type="EnsemblBacteria" id="ADZ09349">
        <property type="protein sequence ID" value="ADZ09349"/>
        <property type="gene ID" value="Metbo_1104"/>
    </dbReference>
    <dbReference type="GeneID" id="10277554"/>
    <dbReference type="KEGG" id="mel:Metbo_1104"/>
    <dbReference type="eggNOG" id="arCOG04748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_2_1_2"/>
    <dbReference type="OMA" id="EYVDRMV"/>
    <dbReference type="OrthoDB" id="4923at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 14" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="428"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="16">
        <location>
            <begin position="613"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="13">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ADZ09349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="653" mass="73965" checksum="28579A8997F759DC" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z2</accession>
    <name>F0T5Z2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1141"/>
    <dbReference type="EnsemblBacteria" id="ADZ09385">
        <property type="protein sequence ID" value="ADZ09385"/>
        <property type="gene ID" value="Metbo_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1141"/>
    <dbReference type="eggNOG" id="arCOG04907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003298_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="277" mass="31164" checksum="A90F813EDADEBBE1" modified="2011-05-03" version="1">MAYLVCDKCGGYYELQPGESPDDFDRCECGGKLKYLTNLDYLSDDFSDYSSSDNSIDAFPKSTLNEREFIKLFWELPIICFFMVGFFVMTYGLSVLSFQFINMHNFMNDFYTAIFIFLILIFFIILCLVGVLVGLKNIRNRYSDWYKEMNWNYRAIGVAFIVTIGVIIFGAKYLPNNFSLIGPLIGGLLAGYMVGINRGMGFVHGGISAGVAGCIGFLLPSLIYKTGLPFLTQTRFDSLILIVLIFAVIYFMVFFILGSIGGTIGTLIKLHSTQKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T634</accession>
    <name>F0T634_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine recombinase XerA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">xerA</name>
        <name evidence="7" type="ordered locus">Metbo_2328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 'phage' integrase family. XerA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2328"/>
    <dbReference type="EnsemblBacteria" id="ADZ10541">
        <property type="protein sequence ID" value="ADZ10541"/>
        <property type="gene ID" value="Metbo_2328"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2328"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_6_2"/>
    <dbReference type="OMA" id="HRFLYAE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02055">
        <property type="entry name" value="Recomb_XerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025269">
        <property type="entry name" value="SAM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033686">
        <property type="entry name" value="XerA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13102">
        <property type="entry name" value="Phage_int_SAM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0229">DNA integration</keyword>
    <keyword evidence="2 3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="140"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="3">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="38520" checksum="5F1D6D938D5836DA" modified="2011-05-03" version="1">MNPYSNQHWKNIEGGSAQLHGTVSSESGHVLRENILDAFDFPEMIEDYLIELEIRNYSKNTIKTYRSIIINFHKFLKSEKDLYDERLVLRAFKRYIRYLKREKNVSQNYIYLVTVVVKKFFEFGGIGVLKDVQTPKRTKSLPKSLNENEILSLINAMDHDKDDYLSETSEFLRLRNKVILALLYSSGLRVSELVTLHSDSFDLTDRTIRIRGKGEKDRIVLFDEQTKELLEGYLQVRGDHSDYLFVNRSGNHLTPRYIQMMIKDYAKAAGIKKKVTPHILRHSFATHLLKNGVDIRAIQQLLGHSNLSTTQIYTSVDMHTLKNVYDRAKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T636</accession>
    <name>F0T636_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name evidence="3" type="ordered locus">Metbo_2330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteropolymeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645894.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2330"/>
    <dbReference type="EnsemblBacteria" id="ADZ10543">
        <property type="protein sequence ID" value="ADZ10543"/>
        <property type="gene ID" value="Metbo_2330"/>
    </dbReference>
    <dbReference type="GeneID" id="10278796"/>
    <dbReference type="KEGG" id="mel:Metbo_2330"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="OMA" id="KYMIVAQ"/>
    <dbReference type="OrthoDB" id="23182at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0271">Exosome</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0639">Primosome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="173"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="41944" checksum="DB70C50C18A33E12" modified="2011-05-03" version="1">MGKEEISTTKYLIHAQINANGIVEKPDVVGAIFGQTEGLLSNDLDLRELQKTGRIGRIKVNINSKAGRSKGEIVIPSSLDRVETAILAASLETINRVGPCEAYIQVSKVEDVRAVKRRKVVDRAKELYKGMMEEVTPESLKMIEEVKEAMRIHEIAEYGDDRLPAGPNVISSDAILVVEGRADVLNLLRYGVKNSIAVEGVSVPKTVAELTKHKTVTAFLDGDRGGDLILKELLQVGEVDYVTRAPRGKEVEDLSKDEVMVALRDKIPVEQIYHDVGVKVEKVEPKGPDKITVLKGILKDLEGSGNAEILDDALNILKEVKVENLYDELKTVNNNAYAVVFDGVISQRLIDMAKEKGLKHIVAVRMSDVVKKPSPIKVITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T666</accession>
    <name>F0T666_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2360"/>
    <dbReference type="EnsemblBacteria" id="ADZ10573">
        <property type="protein sequence ID" value="ADZ10573"/>
        <property type="gene ID" value="Metbo_2360"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2360"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OMA" id="EIYPGFN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="PTHR38136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nre_N" evidence="2">
        <location>
            <begin position="9"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Nre_C" evidence="3">
        <location>
            <begin position="276"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="44077" checksum="F7F71CB7F5833AED" modified="2011-05-03" version="1">MIKGKTAYLKRLTSKMRIPSVDVGKEIDGSTPPSVFIGSWNYPKVYAGPMIAPVSGDTTIMDMPESWIPQSKSQEDIIGYRMSLVRGKQLVGIKDLESGFVEKLQEISLAENSIESEAEFIKKPRGSSFSDEHTPHGPSGIIQKFDIDNVKWEHRLEKAFYDTDLKAADAVVDLNQRGIPFSNIQKAFSVGAMGVGKKRKLVPTRWSITACDSTIADRLLKTVRFNDIIDTTRVYEFSSLNNYYAIMLIPTEWQYEWMEAFLQVLGREELIFSDYETNSGKKEYSRVGGCYYTCKMAVLEALERESKQAGVIVLREAYNGYVPLGVFNVRENVRNAMEQTPLEFEDMKTALSYISTKLELPLEKFVKQSDLMKELLNSRQTTLDSFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T694</accession>
    <name>F0T694_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644760.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09409">
        <property type="protein sequence ID" value="ADZ09409"/>
        <property type="gene ID" value="Metbo_1165"/>
    </dbReference>
    <dbReference type="GeneID" id="10277615"/>
    <dbReference type="KEGG" id="mel:Metbo_1165"/>
    <dbReference type="HOGENOM" id="CLU_2230393_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12100" checksum="1F2A634124791538" modified="2011-05-03" version="1" precursor="true">MLVKKSIILILIVLTFLSPVLAINIDLESTNQDYQTMRNYKNIPDNNLNNAKKYSLIKHNTKRANIHQAKTLKTIDMDCNSVHTSKFDIDATDKKRFEDFNTGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A4</accession>
    <name>F0T6A4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Asp/Glu amidotransferase, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644770.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1175"/>
    <dbReference type="EnsemblBacteria" id="ADZ09419">
        <property type="protein sequence ID" value="ADZ09419"/>
        <property type="gene ID" value="Metbo_1175"/>
    </dbReference>
    <dbReference type="GeneID" id="10277625"/>
    <dbReference type="KEGG" id="mel:Metbo_1175"/>
    <dbReference type="eggNOG" id="arCOG02726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196465_0_0_2"/>
    <dbReference type="OMA" id="MEESYYI"/>
    <dbReference type="OrthoDB" id="127268at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010120">
        <property type="entry name" value="Glu-ADT_subunit_C_archaea"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01827">
        <property type="entry name" value="gatC_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="8269" checksum="AD8CB846A9DD427E" modified="2011-05-03" version="1">MKIEKEAEQILEKFSEALKDIPDLEETHYMVDNVNLTRDDIAEAKDPERIMRNAHTDEDGNIIAEKGKWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A5</accession>
    <name>F0T6A5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="4">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1176</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644771.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1176"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="ADZ09420">
        <property type="protein sequence ID" value="ADZ09420"/>
        <property type="gene ID" value="Metbo_1176"/>
    </dbReference>
    <dbReference type="GeneID" id="10277626"/>
    <dbReference type="KEGG" id="mel:Metbo_1176"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_4_0_2"/>
    <dbReference type="OMA" id="DWSGIYS"/>
    <dbReference type="OrthoDB" id="12911at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="2 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="7">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="359"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="495" mass="55219" checksum="C64EBA33F00E64C3" modified="2011-05-03" version="1">MCAIAGILSINGKQALGEDLICMLKTMEHRGPDGSRICLDGKLVTGDLETTDLPFANIGLGHNLLSIVGTEVSQPLVKHGLVLVANAEIYNYRELKNFYDECYFTDSDCEVILTLVNKYYKNSLVDAVNTTIKELDGDYAFAISNGKELVVVRDELGVKPVYYATDHKRDLFAFASERKALWKLGIKDVNVLKPGEMILNNKTISKPQTKKKTMPIPNVRDGNESFPQYGSNYYKNLLKKVLIDSVKKRVVGLDRVGIIFSGGLDSSILAKLSKDLEVETFLYTVGTENSSDMKYAKQVANSLDLPLKSKIVALDDIKHYTGLVLNAIEEYNVMKIGVGMPSYIASELASQDGIRVMLSGQGADEIFAGYQRYTQFYQEYGEKTTEYLEADVSNLYHVNLQRDDAVTMANSVELRVPYLDSKVVNVGLNIPMKYKLGHDPDDLRKCILRRLALDLEVPMEAVLRPKKAAQYGSGIHRLLVKKVLKDGSYKAQFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I6</accession>
    <name>F0T6I6_METLA</name>
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        <submittedName>
            <fullName evidence="2">2-oxoglutarate synthase</fullName>
            <ecNumber evidence="2">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645971.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2407"/>
    <dbReference type="EnsemblBacteria" id="ADZ10620">
        <property type="protein sequence ID" value="ADZ10620"/>
        <property type="gene ID" value="Metbo_2407"/>
    </dbReference>
    <dbReference type="GeneID" id="10278879"/>
    <dbReference type="KEGG" id="mel:Metbo_2407"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_2_0_2"/>
    <dbReference type="OMA" id="LPTIFCP"/>
    <dbReference type="OrthoDB" id="43666at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
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    </dbReference>
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        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="55"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="31505" checksum="B079CC0851969982" modified="2011-05-03" version="1">MDKTNQNRFMKYLRTERLPNIFCPGCGNGTVMNTFFNALELADMDFDNIALVSGIGCSSRVPGYVDCDSLHTTHGRPITFATGLKLANPDLDVVVFTGDGDAAAIGGNHLIHGSRRNIDITVICINNSIYGMTGGQISPTSPTGSYGSTAPYGALETPFNLSKLTKAAGATYVARWTTAHPVQLSNAIKKGLKNKGFSFIEAISQCPTYFGRKNKMKTAVDMMHWMKDQSVLKRKADKMDPSELEDKLIIGEFVNKTAPEYTEKFCELLEDNCESGKPSSVRSAYRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K0</accession>
    <name>F0T6K0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2421"/>
    <dbReference type="EnsemblBacteria" id="ADZ10634">
        <property type="protein sequence ID" value="ADZ10634"/>
        <property type="gene ID" value="Metbo_2421"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2421"/>
    <dbReference type="eggNOG" id="arCOG10684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599031_0_0_2"/>
    <dbReference type="OMA" id="WLPESNE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031306">
        <property type="entry name" value="CcdC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07301">
        <property type="entry name" value="DUF1453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="19035" checksum="8B5280B6D178F888" modified="2011-05-03" version="1">MIIYPDNSMFTSQSFIIVILFLVILQLRERRVKPLGLMIMPVVMFFISIFLVDEVLFTSLLNFILIVGGFILGTIIGYVVASFMKVKLDKDGNVVMKGSVLAVAVWVLVIILKFYGEQTLGSAHYIDFNVLTSIFIMMTLGAMISRRLIIYRRYREHKLMIKKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L6</accession>
    <name>F0T6L6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="1 2">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gch3</name>
        <name evidence="3" type="ordered locus">Metbo_2437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="1 2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646001.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2437"/>
    <dbReference type="EnsemblBacteria" id="ADZ10650">
        <property type="protein sequence ID" value="ADZ10650"/>
        <property type="gene ID" value="Metbo_2437"/>
    </dbReference>
    <dbReference type="GeneID" id="10278909"/>
    <dbReference type="KEGG" id="mel:Metbo_2437"/>
    <dbReference type="eggNOG" id="arCOG04202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="OMA" id="VQIAHID"/>
    <dbReference type="OrthoDB" id="56576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="PTHR42202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="27911" checksum="9CD3D72531982269" modified="2011-05-03" version="1">MIQMTLIQIDNYGPWTVTPTPRAEADLQILQSELYADLQRQFAAKGGLVFFTRFDNMLAVTNGVDMEDHKRIQKSIGNRYPITISMGVGAAETPYEAQRKATNALQNYGGAQSEDRTEVLAIDGLVKADESFVQIAHIDINGITDSLTDIIPAYDTSFIVNRVQHFLMKKLIEKGSLLFFIGGDNFMSPCNGLNPEGLLKIIEEIEDEINIALKAGVGKAPTAEKAANLADLALEEIRGGFTYNLVHVMKNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P8</accession>
    <name>F0T6P8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643632.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0028"/>
    <dbReference type="EnsemblBacteria" id="ADZ08281">
        <property type="protein sequence ID" value="ADZ08281"/>
        <property type="gene ID" value="Metbo_0028"/>
    </dbReference>
    <dbReference type="GeneID" id="10276450"/>
    <dbReference type="KEGG" id="mel:Metbo_0028"/>
    <dbReference type="eggNOG" id="arCOG10459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168019_0_0_2"/>
    <dbReference type="OrthoDB" id="115155at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13059" checksum="8098E0FD8DABD90E" modified="2011-05-03" version="1">MQVGGQGFHGGNGTEAGKGLGLQLANSQIITIDIIIGIGNICLLIFLLFMYLRSYRDFKSKFTFGLVAFALLLLLQNILFTGFLLTYQGFRGPGMGAPIFFLNIIEFFALSILIGVTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q8</accession>
    <name>F0T6Q8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643642.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0038"/>
    <dbReference type="EnsemblBacteria" id="ADZ08291">
        <property type="protein sequence ID" value="ADZ08291"/>
        <property type="gene ID" value="Metbo_0038"/>
    </dbReference>
    <dbReference type="GeneID" id="10276460"/>
    <dbReference type="KEGG" id="mel:Metbo_0038"/>
    <dbReference type="eggNOG" id="arCOG00663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_2_2"/>
    <dbReference type="OMA" id="YWRAIDT"/>
    <dbReference type="OrthoDB" id="78828at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="335" mass="38705" checksum="736FF51C0A90CD27" modified="2011-05-03" version="1">MTRTVGMILCGGFGKRLRPLTETVPKPLIEIKDNYTILDKQLFDFKNAGVDKVLLLTGFLSEKIRERYGDEYKGVKIEYIVEDEPLGTLNAIKLGMESLEDNEQCIIRNGDVVADLNIKKMIEQGEKSDYPLSIFITQMVSPYGIVEISGDRLVSFKEKPVLDYYINGGVYFSNGHIDFGSFEVGDIEKTVFPMLAKDNKLGYYKENGLFWMAIDTSKELEAIKKEYENREDKPWGYEKILINTDKYLTKELFIREGYQTSFHYHSKKDETMYIVSGAGYIEFNDRKDYFGKNDTIRIEPEEEHSIVATENTVLHEVSTPHLNDTIRVKDYYKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S4</accession>
    <name>F0T6S4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643658.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0054"/>
    <dbReference type="EnsemblBacteria" id="ADZ08307">
        <property type="protein sequence ID" value="ADZ08307"/>
        <property type="gene ID" value="Metbo_0054"/>
    </dbReference>
    <dbReference type="GeneID" id="10276477"/>
    <dbReference type="KEGG" id="mel:Metbo_0054"/>
    <dbReference type="eggNOG" id="arCOG01579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052165_0_0_2"/>
    <dbReference type="OMA" id="GISYCNP"/>
    <dbReference type="OrthoDB" id="66892at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="29419" checksum="7D6BEA9146AB44F9" modified="2011-05-03" version="1">MKAVVFDNSGTLIRRYKALKDLRNGLICDYANSIQIVDHDINRALVVLQTDPSKCIIKANPNQTIHDFLMKNDVQFDISYSDVDIAKSDLLKSIENDDSTMKDLQDTYNAVIDKKYNVHICSGSGFIVNMKTGRVEFTITAGGKIFKEVPDVISELKNRNIQIFVASGDRKGSLEQLAEYIHIPKENVFDTASSRQKKEIIDSLKKRYRVMMVGNSSNDILALKEADIGVLTLQQGDETPDKVFNAADHVINNISEILNVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X9</accession>
    <name>F0T6X9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644850.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1257"/>
    <dbReference type="EnsemblBacteria" id="ADZ09499">
        <property type="protein sequence ID" value="ADZ09499"/>
        <property type="gene ID" value="Metbo_1257"/>
    </dbReference>
    <dbReference type="GeneID" id="10277708"/>
    <dbReference type="KEGG" id="mel:Metbo_1257"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_3_1_2"/>
    <dbReference type="OMA" id="YRWEEST"/>
    <dbReference type="OrthoDB" id="105754at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="12427" checksum="C899826494B76388" modified="2011-05-03" version="1">MNAQFKKGVLELCVLVLLDRKDCYGYEMVDEISKNISISEGTIYPLLRRLKKENLVSSYLKESQDGPPRKYYRITELGKEKKENLVAEWDTFSAAVKNLLNYKTATGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z1</accession>
    <name>F0T6Z1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644862.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1269"/>
    <dbReference type="EnsemblBacteria" id="ADZ09511">
        <property type="protein sequence ID" value="ADZ09511"/>
        <property type="gene ID" value="Metbo_1269"/>
    </dbReference>
    <dbReference type="GeneID" id="10277720"/>
    <dbReference type="KEGG" id="mel:Metbo_1269"/>
    <dbReference type="eggNOG" id="arCOG00628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286363_0_0_2"/>
    <dbReference type="OMA" id="AIWACGG"/>
    <dbReference type="OrthoDB" id="61989at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016486">
        <property type="entry name" value="UCP006591_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006591">
        <property type="entry name" value="UCP006591_CBS_MJ1004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="68"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="23644" checksum="2030DE4DC31612C1" modified="2011-05-03" version="1">MKVKEIMDKNFIGVDLNMTIVDASIKMESHKKFTTPVLDSKNTLIGWITSLDVTRGLRENHKYVKDIMHSTEEIVDLHQDEPARLAVLAAAQHKLVSIPVLDDDGKVVGVVRTFDIVKTLSKLYEIKVTNIFKAMSTELKGVSWDELMEASAIVTKRETGKRVTAKEYEQRIKNSTFGEAIWATGGLEKFFVGLIAIGELVIARKVAKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T709</accession>
    <name>F0T709_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644880.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1287"/>
    <dbReference type="EnsemblBacteria" id="ADZ09529">
        <property type="protein sequence ID" value="ADZ09529"/>
        <property type="gene ID" value="Metbo_1287"/>
    </dbReference>
    <dbReference type="GeneID" id="10277738"/>
    <dbReference type="KEGG" id="mel:Metbo_1287"/>
    <dbReference type="eggNOG" id="arCOG04829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170806_0_0_2"/>
    <dbReference type="OMA" id="LEIGMIG"/>
    <dbReference type="OrthoDB" id="120353at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019213">
        <property type="entry name" value="DUF2108_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09881">
        <property type="entry name" value="DUF2108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10329" checksum="1F567E553AE180AA" modified="2011-05-03" version="1">MYPFWDLINLSTVSAGLALIGAAGIIMLKKPIDKVIMLALLEAGLIGMIVAAKYLDVAMAGAIFDPISTIILLIAITKINEIREKKIKSQGEKFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T712</accession>
    <name>F0T712_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">One of the integral membrane subunits of multisubunit membrane-bound [NiFe]-hydrogenase eha. Eha is predicted to form large electron transfer complex and might catalyze energy-driven reduction of low-potential redox carriers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Putative multisubunit membrane-bound [NiFe]-hydrogenase eha is composed of at least 20 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EhaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644883.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1290"/>
    <dbReference type="EnsemblBacteria" id="ADZ09532">
        <property type="protein sequence ID" value="ADZ09532"/>
        <property type="gene ID" value="Metbo_1290"/>
    </dbReference>
    <dbReference type="GeneID" id="10277741"/>
    <dbReference type="KEGG" id="mel:Metbo_1290"/>
    <dbReference type="eggNOG" id="arCOG05035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174516_0_0_2"/>
    <dbReference type="OMA" id="MTAYFLE"/>
    <dbReference type="OrthoDB" id="101707at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011306">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17367">
        <property type="entry name" value="NiFe_hyd_3_EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="10618" checksum="1A3069005E119A46" modified="2011-05-03" version="1">MIIHANVILISSYAVAVISSIVVGLLLRIPLLPERPMRQSWTISIVFPTAVLALGFSAMVFKLGVDGLLVAAIIGIITALFSKYILERVLPEPVMEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T774</accession>
    <name>F0T774_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2495"/>
    <dbReference type="EnsemblBacteria" id="ADZ10708">
        <property type="protein sequence ID" value="ADZ10708"/>
        <property type="gene ID" value="Metbo_2495"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2495"/>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413137_0_0_2"/>
    <dbReference type="OMA" id="TIGENFA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="Beta_helix" evidence="3">
        <location>
            <begin position="171"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="664" mass="71018" checksum="B69ABBB0B575D80D" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T787</accession>
    <name>F0T787_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formylmethanofuran dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646072.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2508"/>
    <dbReference type="EnsemblBacteria" id="ADZ10721">
        <property type="protein sequence ID" value="ADZ10721"/>
        <property type="gene ID" value="Metbo_2508"/>
    </dbReference>
    <dbReference type="GeneID" id="10278982"/>
    <dbReference type="KEGG" id="mel:Metbo_2508"/>
    <dbReference type="eggNOG" id="arCOG00097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072248_0_0_2"/>
    <dbReference type="OMA" id="VNGDAGM"/>
    <dbReference type="OrthoDB" id="72252at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="29665" checksum="A2C38214316CAA0A" modified="2011-05-03" version="1">MKTITFNQKKTSQISLEFDELIPDEIYSWEKADFEKYMVPIGNSRFPLTDYFDIEVEGSAESPEEIKMILNGDYGRVKYIGTKMGAGEIIVNGPVDLHCGSQMKGGSILVNGDAESYAGREMEGGSLTIMGNTREFCGCSYIGDWRGMNGGTITIHGNAGKQLGECLSGGSIYVKGDCDILAGIHMTKGFIQIDGDVTRWPGGQMKNGDIVINGELGRLLEGFVQEDIIENPEIDGKDFSGKYIKYVGDIALNGKGRLWLDAEKNRHLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T790</accession>
    <name>F0T790_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin dinucleotide-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2511"/>
    <dbReference type="EnsemblBacteria" id="ADZ10724">
        <property type="protein sequence ID" value="ADZ10724"/>
        <property type="gene ID" value="Metbo_2511"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2511"/>
    <dbReference type="eggNOG" id="arCOG02674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123704_0_0_2"/>
    <dbReference type="OMA" id="GMIYIPM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molydop_binding" evidence="1">
        <location>
            <begin position="29"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="19910" checksum="979618AE312B4318" modified="2011-05-03" version="1">MTYVDKPAIPKVVKFDEPNAKLRNKLDVMLNTGSDIYQGACKKRGSTLKDEYRKVAGVAYMDPKDMAKLGVSNWDHVKVISDWGEVVVNAAHSRDAPHEGTIFIPKGPWANVVVSPETYCCCDPTYKGIMCTVEKSDDDVLLMADLMRAVYKKYVNKDDEEGIKDLATLGEMPVYKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0T796</accession>
    <name>F0T796_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="9">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">moaA</name>
        <name evidence="11" type="ordered locus">Metbo_2517</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646081.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2517"/>
    <dbReference type="EnsemblBacteria" id="ADZ10730">
        <property type="protein sequence ID" value="ADZ10730"/>
        <property type="gene ID" value="Metbo_2517"/>
    </dbReference>
    <dbReference type="GeneID" id="10278991"/>
    <dbReference type="KEGG" id="mel:Metbo_2517"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="OrthoDB" id="18533at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="13"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="262"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="320" mass="37006" checksum="E2FABF27634ACEC6" modified="2011-05-03" version="1">MKTINIQNKVTDNFKRPLISLRISITNRCNVKCFYCHHDGIVPQDYEMTPKEIERIVTVAKELGIEKIRLSGGEPLIREDIVDMVSKIANVGFRDISLTTNGILLEKYAEKLHEAGLTRVNVSFDTLNPETYRFITKRDYMENAKAGIQKAVESGLNPVKVNMVVMKGINDNEIWDMFQFCRETGAILQLIELLKTEADKPGSFFDEYHYDMGELEDELTEMSDQVKTRKFMQDRKKYFVEGGEIEIVRPMDNTEFCKNCTRLRITPDGKIKPCLLKNDNLVDIIEPMRRGYTNKELKNVFLEAIEHRKPYFTNGCHAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C0</accession>
    <name>F0T7C0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="4">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">surE</name>
        <name evidence="6" type="ordered locus">Metbo_0096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643700.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0096"/>
    <dbReference type="EnsemblBacteria" id="ADZ08349">
        <property type="protein sequence ID" value="ADZ08349"/>
        <property type="gene ID" value="Metbo_0096"/>
    </dbReference>
    <dbReference type="GeneID" id="10276519"/>
    <dbReference type="KEGG" id="mel:Metbo_0096"/>
    <dbReference type="eggNOG" id="arCOG02303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_2"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="OrthoDB" id="110625at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SurE" evidence="5">
        <location>
            <begin position="3"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="27835" checksum="EA1A87A5E34E95EE" modified="2011-05-03" version="1">MRILITNDDGVNSSGIVAAKRAVENLGTIDVVAPATQQSGIGHALTLFEPIRVTSTNLMDGTEAFSVSGTPTDSVIIGIYEVTESKPDLVISGINIGENLGKSELTTSGTIGAAMEAATHGIPAISVSLQVSRGDIKFHDGHVALDFRYSEKILNSVAKNVIKKGLPEGADFINLNIPSHPASDKIKCTRLGERMYGIHIQKRLDPRGRPYYWIDGDPVENDEYGTDVHTLKNDRCPTITPISLDCTSDMRSMDGWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F1</accession>
    <name>F0T7F1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643731.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0127"/>
    <dbReference type="EnsemblBacteria" id="ADZ08380">
        <property type="protein sequence ID" value="ADZ08380"/>
        <property type="gene ID" value="Metbo_0127"/>
    </dbReference>
    <dbReference type="GeneID" id="10276552"/>
    <dbReference type="KEGG" id="mel:Metbo_0127"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_878823_0_0_2"/>
    <dbReference type="OMA" id="NEYGSSW"/>
    <dbReference type="OrthoDB" id="36417at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="316" mass="35309" checksum="F50AE21D86C565CD" modified="2011-05-03" version="1">MSLVINTTTPEGIVLAADSRQSYRNLKGMARIGSDNAMKLFQINSRIGVGITGLAFLEEEGVLKNMSKFIEEFKRGPEIEELDVEDVAVELYKLFNEKYHWKEQLDMAEENIRNDLLSKGCELLNIEKNDYNINFSFKNPQGMIEEGVAGVDMIELLVAGYNQNGTHQVYGCYIPGEIQKRRDSLDRNNEYGSSWIGQGDVVSRIVLGFDGRIANVAYTKEAMLIKGEAQVFEELKNLEYAIQWGTMTLQDGIDFCTLMIQTTSAMQRFSDGINADPGDMPGVGGPIDVAVITPDNGFVWINKKKLSYGENQVELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I0</accession>
    <name>F0T7I0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Metbo_1306"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_1306"/>
    <dbReference type="eggNOG" id="arCOG01403">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ALG2"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="221"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="46215" checksum="57D1C2AE9AF02722" modified="2011-05-03" version="1">MKIAVFHNLPSGGAKRALYGTVDYLIHKGHNVEAYLPSTANEDFLPLSELVDEVHLYHVKNTIGGWLYSSFKYVPPFVKTISLRDLEATQRDIAGIINDSDHDVVLCEQDQYTLSPFFLKYIKKPTVYYCQQPTRDEAILKKLEKMVENQPNKLKGAVFNYSDKKDLKIDMENAQHAKYILANSYFSRESILKGYGLNSYVSYLGIDTNIFRRMEVPVEDHVLSVGTITPTKGYDFLIKSLSLVKPEVRPKLIIAANHSVLEWKNYIVELAKQLDVNLEIMDMVDDEKLVELYNTSKLVLYAPYLEPFGLVPIEAMGCGTPVIGVKEGGVRETVLENQTGILVERDPQKFADAITQTLNNPTQLYDMGRNGIKEVERFWTLEHAGERIEKHLENAIKTHEHSQKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L4</accession>
    <name>F0T7L4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Resolvase helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_1342"/>
    <dbReference type="EnsemblBacteria" id="ADZ09582">
        <property type="protein sequence ID" value="ADZ09582"/>
        <property type="gene ID" value="Metbo_1342"/>
    </dbReference>
    <dbReference type="GeneID" id="10277793"/>
    <dbReference type="KEGG" id="mel:Metbo_1342"/>
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    <dbReference type="OMA" id="EYHYYKL"/>
    <dbReference type="OrthoDB" id="106282at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_7" evidence="1">
        <location>
            <begin position="109"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17558" checksum="D1C99FE86F67776C" modified="2011-05-03" version="1">MTEILYINEPLSSRKIMEVLDNNPDLKKINCPISIYSRIAKKYIEALDELGIEVEPVLKRGRPKKYSSSEASKIQVMLDKGESPKEISKKLNLPVKTVYYLKNSSLKRGRKVKYSAEKTKEVKRLYSKGMPAKKISEKLKIPLRTVYLLLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L5</accession>
    <name>F0T7L5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644934.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1343"/>
    <dbReference type="EnsemblBacteria" id="ADZ09583">
        <property type="protein sequence ID" value="ADZ09583"/>
        <property type="gene ID" value="Metbo_1343"/>
    </dbReference>
    <dbReference type="GeneID" id="10277794"/>
    <dbReference type="KEGG" id="mel:Metbo_1343"/>
    <dbReference type="eggNOG" id="arCOG03198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_531708_0_0_2"/>
    <dbReference type="OMA" id="HKIAIGN"/>
    <dbReference type="OrthoDB" id="21208at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007503">
        <property type="entry name" value="DUF530"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04409">
        <property type="entry name" value="DUF530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="522" mass="60672" checksum="E0ACED784D27C6DB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N4</accession>
    <name>F0T7N4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="179" mass="20154" checksum="337601A72B25264E" modified="2011-05-03" version="1">MIKISYDIKVYRQELINNIKKADVVVELGCHVGNSTAIIAELSPNGRVIALDNSPEAITPMKLLEENNSNINFYKADVRLHETLEMVAKQIKEIGRCDVLSVDLGGGYHPDTVFKVFFIWSSTLKPRLTIIRNRGLVDFIHSAQAEEEIKSQEGWLESSGNDGIPPRLKEFKLWSSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q6</accession>
    <name>F0T7Q6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphomethylpyrimidine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2525"/>
    <dbReference type="EnsemblBacteria" id="ADZ10738">
        <property type="protein sequence ID" value="ADZ10738"/>
        <property type="gene ID" value="Metbo_2525"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2525"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="OMA" id="FMAIHTG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="429" mass="46412" checksum="E273B660416B41C4" modified="2011-05-03" version="1">MDDAKKGIITEQMKAVAKIENVDEEFIRKSVANGTIAIPNNVGRDVKPVGIGAGLRTKVNATIGTSTDINDIDMEVEKANIAMENGADTLMELSVGGDLDGIRRKILSITDLPVGSVPLYQASIECIREHGAAIYMEEDAVFKAIETQAKDGIDFMAIHCSVNKETLKRLKRQGREGGLVSRGGAFMSAWMVHNETENPLYKDYEYVLDIAKEYDFVISLANGMRAGAIADSTDRAQVQELIVLGELVDRARERGVQTIVEGPGHIPLKEISTNVMVQKKLCKNAPFYMLGPIVTDIAPAYDHIVSSIGAAQSASAGADFICYVTPAEHLALPGPEDVKTGLIASRIGAYVGDMSKGIHNGELDLVMANARKKLDWEGQYSASICPADARAIRDAKPPEDPDTCTMCGSYCAVKLVNEWLDDASKDVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R7</accession>
    <name>F0T7R7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643755.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0152"/>
    <dbReference type="EnsemblBacteria" id="ADZ08404">
        <property type="protein sequence ID" value="ADZ08404"/>
        <property type="gene ID" value="Metbo_0152"/>
    </dbReference>
    <dbReference type="GeneID" id="10276578"/>
    <dbReference type="KEGG" id="mel:Metbo_0152"/>
    <dbReference type="eggNOG" id="arCOG01880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031477_4_2_2"/>
    <dbReference type="OMA" id="CKREKED"/>
    <dbReference type="OrthoDB" id="91246at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="PTHR31303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20566" checksum="156FCD1956B4C7C6" modified="2011-05-03" version="1">MDKELVRQLIHASGVFVLILGLFLRIDILILLCVIMVVSVEIMFVLDKYMHIPVFSNIMSTCKRSEDERGFLYFFIGIIATLVIFSFNLTIAYSAILLLLIGDSLSTIIGKRFGNHKLPFNQSKSFEGSLAFFGAGLICCLIFLQPIPAVIGALAGTLTEAYSPIDDNIPIPLISAMIMTIVIYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V0</accession>
    <name>F0T7V0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643788.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0185"/>
    <dbReference type="EnsemblBacteria" id="ADZ08437">
        <property type="protein sequence ID" value="ADZ08437"/>
        <property type="gene ID" value="Metbo_0185"/>
    </dbReference>
    <dbReference type="GeneID" id="10276611"/>
    <dbReference type="KEGG" id="mel:Metbo_0185"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_2_2"/>
    <dbReference type="OMA" id="AGDENYI"/>
    <dbReference type="OrthoDB" id="97695at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16695" checksum="A7BEC8E180A958AC" modified="2011-05-03" version="1">MDDIDSEIIRSLVRNSRITLSQMSKEIDVPDATISNRLKKLENNVIKQYTLIVNPEEIGLTVTAIIIIQTESEKHENVKYELSKLEEVSEVYTVSGEYDILIKAWAHGIEELNEIMNVKIRSIDGVEDLTEMIVLERVKEDVIPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V4</accession>
    <name>F0T7V4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="gene ID" value="Metbo_0189"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0189"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="121070at2157"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10913" checksum="5EBEEAA877A4777A" modified="2011-05-03" version="1">MNPVNSYSRKISKKEADKGFIFILKSKLSYFPKSSFTLSDGDITEVREIKSYPCTCRGPDRPHDHYYISWDNLEVGNIIEIRKNDSGDYMITQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z4</accession>
    <name>F0T7Z4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_1380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ09620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09620">
        <property type="protein sequence ID" value="ADZ09620"/>
        <property type="gene ID" value="Metbo_1380"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_1380"/>
    <dbReference type="eggNOG" id="arCOG10255">
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    </dbReference>
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    <dbReference type="OMA" id="ANACFLI"/>
    <dbReference type="OrthoDB" id="126949at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="112"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="17074" checksum="3D5E0D12B8D82E2A" modified="2011-05-03" version="1">MAEDINNILSGIKRSLKEDKEEKWYDYLFAILLNLILLYIANNLIYWNLSFIAASFSNVLWAVNLSLIVAVVANVSFILYDPRWFKICSKIIMNITALVAAFTLLTVFPFVINQFILVLGLKIILIIGILASVVSILFQIMKLAYLMASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T809</accession>
    <name>F0T809_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="GeneID" id="59558430"/>
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    <dbReference type="eggNOG" id="arCOG10883">
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    <dbReference type="HOGENOM" id="CLU_2140212_0_0_2"/>
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12973" checksum="A2DB5557C17ED867" modified="2011-05-03" version="1">MVSNNEIKQILSERRQGIFRNGYLVCDTCQGVYELKDGEKPDDFSEECECGGHLSYTRTLELGEEDPDEDFFDSPTLNWLSKRRNILKIRIVLSTITFLFIGAFLLIMILMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T812</accession>
    <name>F0T812_METLA</name>
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        <recommendedName>
            <fullName evidence="1">Ferritin domain-containing protein</fullName>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644989.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1398"/>
    <dbReference type="EnsemblBacteria" id="ADZ09638">
        <property type="protein sequence ID" value="ADZ09638"/>
        <property type="gene ID" value="Metbo_1398"/>
    </dbReference>
    <dbReference type="GeneID" id="10277849"/>
    <dbReference type="KEGG" id="mel:Metbo_1398"/>
    <dbReference type="eggNOG" id="arCOG01094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891423_0_0_2"/>
    <dbReference type="OMA" id="DEMRHMW"/>
    <dbReference type="OrthoDB" id="104399at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
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        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin" evidence="1">
        <location>
            <begin position="9"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="16084" checksum="1002BC2C4EED301C" modified="2011-05-03" version="1">MKNDEIIRFLNEDFVRELEATMIYVYNSFVMEECDPSRVTEAISVDEMRHMWWLSDLITKRGGKPTMEHKELDFGGKDLKSQLEHQIQLETNGINRYTEQIKIIDDEEVVGVLKHILDEEKRHRKEFKTRLEGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T828</accession>
    <name>F0T828_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1415</name>
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    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645005.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09654">
        <property type="protein sequence ID" value="ADZ09654"/>
        <property type="gene ID" value="Metbo_1415"/>
    </dbReference>
    <dbReference type="GeneID" id="10277866"/>
    <dbReference type="KEGG" id="mel:Metbo_1415"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021523_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13984" checksum="88A4E129C9DD2C14" modified="2011-05-03" version="1">MVYCPVCGTENPKDVKECKNCHANLDNILNNIKSEESEYNLLKISGYVFIILGLFSLGTLSIVGLILGYKTYQQNNSNAKTHGIIIVVLNIVVILFWIWTLFLVPQWWQSTSLNQSIPRFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T850</accession>
    <name>F0T850_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1438"/>
    <dbReference type="EnsemblBacteria" id="ADZ09676">
        <property type="protein sequence ID" value="ADZ09676"/>
        <property type="gene ID" value="Metbo_1438"/>
    </dbReference>
    <dbReference type="GeneID" id="10277889"/>
    <dbReference type="KEGG" id="mel:Metbo_1438"/>
    <dbReference type="eggNOG" id="arCOG03038">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_14_3_2"/>
    <dbReference type="OrthoDB" id="89899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="19912" checksum="61E261C5E562853B" modified="2011-05-03" version="1">MDEQDVEVLYKQALSYMEQGDKQMAIEFFNKAIAMDPNYSPAWNDKGIVHMELKEFDEAFKCFDTAMRIDSSNSMPVYNMGYVLLMQEKYEDAVHAFDMFLERYPDEKNEFYKYGLYLKAEAHYKLKQYDQAKNLLDKAIKRDRIFKEARDLMIKILQDEKNIKSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T881</accession>
    <name>F0T881_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08493">
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        <property type="gene ID" value="Metbo_0241"/>
    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976551_0_0_2"/>
    <dbReference type="OMA" id="VEIHFMG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021598">
        <property type="entry name" value="DUF3221"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11518">
        <property type="entry name" value="DUF3221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="13199" checksum="AAE31127AAF56BEF" modified="2011-05-03" version="1" precursor="true">MKNLILIVLLFVMFMGVVYGAMVATGGENWDMTGKIVGVCTADNNSTDANSILVQGTVISGNQQANVTVRITNDTQLFSKNGNQRNQINFSDLKSGQSVSIQFKGPILQSYPPQASGSLVLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B2</accession>
    <name>F0T8B2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643875.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0272"/>
    <dbReference type="EnsemblBacteria" id="ADZ08524">
        <property type="protein sequence ID" value="ADZ08524"/>
        <property type="gene ID" value="Metbo_0272"/>
    </dbReference>
    <dbReference type="GeneID" id="10276704"/>
    <dbReference type="KEGG" id="mel:Metbo_0272"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="OMA" id="WNPREAD"/>
    <dbReference type="OrthoDB" id="100307at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="15931" checksum="129FE91C81B1E636" modified="2011-05-03" version="1">MSLKSYSRARAVHVMLVYTGGCNGCDIEIVNCVLSPKYDAEQYKVFLTWNPREADVLVVTGPVTRHNEKPLREIYDAIPEPKAVIAAGACALMGGVYKNIHGDIPSEEIMGPVDNIIPVDAKVPGCAVRPEDVLSGVVSALPLLLDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D2</accession>
    <name>F0T8D2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyltetrahydropterin synthase</fullName>
            <ecNumber evidence="5">4.2.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0292</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643895.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0292"/>
    <dbReference type="EnsemblBacteria" id="ADZ08544">
        <property type="protein sequence ID" value="ADZ08544"/>
        <property type="gene ID" value="Metbo_0292"/>
    </dbReference>
    <dbReference type="GeneID" id="10276724"/>
    <dbReference type="KEGG" id="mel:Metbo_0292"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_3_0_2"/>
    <dbReference type="OMA" id="IGQGACY"/>
    <dbReference type="OrthoDB" id="104644at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="17848" checksum="868033EADA79F515" modified="2011-05-03" version="1">MKIVINGIHANLRFSAAHMIPEHESCGVIHGHSYIVDVVVEGDRSGEFGFVVDFKEVKKIVRDLCKKMDHRVLIPLKNPKINFTNIEGPIEFSIGDKNYMLPNEDCMLLDLKSTSAEELSEYFATELYGSLIKINPGISSVQICVNEGIGQGAYFTKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H6</accession>
    <name>F0T8H6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645078.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09727">
        <property type="protein sequence ID" value="ADZ09727"/>
        <property type="gene ID" value="Metbo_1495"/>
    </dbReference>
    <dbReference type="GeneID" id="10277946"/>
    <dbReference type="KEGG" id="mel:Metbo_1495"/>
    <dbReference type="eggNOG" id="arCOG02572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_4_2_2"/>
    <dbReference type="OMA" id="YFEYTAG"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="20185" checksum="DC31D3559492AB01" modified="2011-05-03" version="1">MKVLGLVGSPREGGNTEIMVKEILEESSENGAETKIYNLNELDIKPCQACMHCKSHDGECKTDDDMQILYKEMAESDAFVLGSPIYMWQMTAQAKLFTDRLYALMGFPEKLGETSSALVFTQGNPDGDLFEDYINSTKQVFDLLGYPVKGLLVSAGDQTLGDVKNKEDILEKAREIGKDLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M4</accession>
    <name>F0T8M4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="8">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gatB</name>
        <name evidence="10" type="ordered locus">Metbo_0314</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643917.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0314"/>
    <dbReference type="EnsemblBacteria" id="ADZ08566">
        <property type="protein sequence ID" value="ADZ08566"/>
        <property type="gene ID" value="Metbo_0314"/>
    </dbReference>
    <dbReference type="GeneID" id="10276751"/>
    <dbReference type="KEGG" id="mel:Metbo_0314"/>
    <dbReference type="eggNOG" id="arCOG01718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_1_2"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="21200at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="9">
        <location>
            <begin position="303"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="452" mass="50957" checksum="67DDC65F36BD38CC" modified="2011-05-03" version="1">MKCGLEIHVQLKTDSKLFCSCHTNYKDAKANTNICPVCLNQPGAKPYPPNKAALDAAIKIALMLGCDISPEVTYFQRKHYDYPDLSSGYQRTSIPIGSGGELNGVRIYDVHIEEDPGQYKPDMGTVDFNRSGIPLIEIVTHPDMKSPEEARKFLRELIRVLDYSGSARGEGSMRADVNISIEGGKRAEIKNINSIKGAYKALQFEMVRQKNLLKRGIEIKQETRAFLESQMITVPMRLKEEAEDYRYIPDPDLPPMQAEEDAVEIIREAMPEPAHIKTERFVNEYGISRDHAKVITSELELADAFEEVANSVDPEFAALWMRDELKRVIYYNKMSFKESEITASQIVDLLKMLQNKKITAKAGKRIMEKLPRNSQMPSTIAEEMGLTGVVTEDQVVDAIKQVVKENPDAVSDYFEGKKNAINFLVGQVMRLTRGKADPTETNKLLEVELNSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S1</accession>
    <name>F0T8S1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetrahydromethanopterin S-methyltransferase subunit H</fullName>
            <ecNumber evidence="3">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mtrH</name>
        <name evidence="4" type="ordered locus">Metbo_0361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step. MtrH catalyzes the transfer of the methyl group from methyl-tetrahydromethanopterin to the corrinoid prosthetic group of MtrA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the MtrH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643964.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0361"/>
    <dbReference type="EnsemblBacteria" id="ADZ08613">
        <property type="protein sequence ID" value="ADZ08613"/>
        <property type="gene ID" value="Metbo_0361"/>
    </dbReference>
    <dbReference type="GeneID" id="10276798"/>
    <dbReference type="KEGG" id="mel:Metbo_0361"/>
    <dbReference type="eggNOG" id="arCOG04336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048697_0_0_2"/>
    <dbReference type="OMA" id="YARHKIV"/>
    <dbReference type="OrthoDB" id="86442at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01501">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023467">
        <property type="entry name" value="MeTrfase_MtrH/MtxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028342">
        <property type="entry name" value="MtrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02007">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500206">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004960">
        <property type="entry name" value="MtrH_MtxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01114">
        <property type="entry name" value="mtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2 3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="310" mass="33299" checksum="0C3C25E470FC3083" modified="2011-05-03" version="1">MFRFDKEQVVLDVAGLKVGGQPGEYPTVLAGTIFYGGHKIISDEKAGTFDKDKAEGLIKMQEEMADVTGNPHIVHTFGATPEALVKYLEFVGDTTDAPFFIDSTSGEARMAGAKYVTETGLADRGIYNSLNMSADEEELKVLTECDITSSIVLGFNPMEAGVEGKINIWDNGGSVLDKGLLDMAADCGIDKPFMDVAVTPLGQGAGPALRTSFAVKSKWGYPVGSGIHNVPSAWDWLRGYKKENREVWPVSDIGSNLLQVAAGGDFVLMGPIENAKMAFTAVAMTDMFMAEAAKDIGTEPIETHPFNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S4</accession>
    <name>F0T8S4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acylneuraminate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0364"/>
    <dbReference type="EnsemblBacteria" id="ADZ08616">
        <property type="protein sequence ID" value="ADZ08616"/>
        <property type="gene ID" value="Metbo_0364"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0364"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_0_0_2"/>
    <dbReference type="OMA" id="GMGEKPC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="220" mass="24374" checksum="6A5ABD9F44395FB4" modified="2011-05-03" version="1">MYTGTQKEITEEIRKWCDYMVKAIVMAGGKGTRMEGDSEKPLKEVFKKPLILHVLKALKGSKNIKDIIIATSANTPETAKVVGNWGFKVVETPGNGYVEDLQYVIQKCYSDDDETILTTTSDLPLINADIIDEVIAAYNNSPKPAMCVAVPEDIFQEYGLKPSIVLDDVVPSGLNILRSNNKQQDEEVLKLRKIELAVNINSCEDIVVLEEVLSNIGIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T905</accession>
    <name>F0T905_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0375</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643978.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0375"/>
    <dbReference type="EnsemblBacteria" id="ADZ08627">
        <property type="protein sequence ID" value="ADZ08627"/>
        <property type="gene ID" value="Metbo_0375"/>
    </dbReference>
    <dbReference type="GeneID" id="10276812"/>
    <dbReference type="KEGG" id="mel:Metbo_0375"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_1_0_2"/>
    <dbReference type="OMA" id="IREFHRH"/>
    <dbReference type="OrthoDB" id="116879at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28987" checksum="530EB24E3895032C" modified="2011-05-03" version="1">MAVKEGDFVKIGYTAKIQETGDVFDTTDETAAKEAGIHVENKSYGPIPIVVNGGHVLKGLDEALVGMEEGEEKTVEIAPENGFGMRDPKLMQLIPMSEFRKQGMKPEVGMAISSEGTTGIIRSISGGRVTVDFNHELAGKNLVYDIKVEDVIEDDVEKVKSMIELHYKAPNLDSDKHDIQIEDGVVRIAMDEMAKFDKQPYMDVTFARFRISRDIWENIENIEKVEFVDVFEKKVEEAAEEVVAEEVAEEVLTDEDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T907</accession>
    <name>F0T907_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643980.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0377"/>
    <dbReference type="EnsemblBacteria" id="ADZ08629">
        <property type="protein sequence ID" value="ADZ08629"/>
        <property type="gene ID" value="Metbo_0377"/>
    </dbReference>
    <dbReference type="GeneID" id="10276814"/>
    <dbReference type="KEGG" id="mel:Metbo_0377"/>
    <dbReference type="eggNOG" id="arCOG01354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184946_0_0_2"/>
    <dbReference type="OMA" id="VENESMI"/>
    <dbReference type="OrthoDB" id="129264at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8725" checksum="B4F420B845352F79" modified="2011-05-03" version="1">MKITAKIEFRYINPEYAEAAYESLHPDNVGYIESYVENESMICCIEGDSVGRLLATADDLIFCETMVEKIAELTKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T911</accession>
    <name>F0T911_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable L-tyrosine/L-aspartate decarboxylase</fullName>
            <shortName evidence="3">TDC/ADC</shortName>
            <ecNumber evidence="3">4.1.1.11</ecNumber>
            <ecNumber evidence="3">4.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mfnA</name>
        <name evidence="5" type="ordered locus">Metbo_0381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis. Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + L-aspartate = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + L-tyrosine = CO2 + tyramine</text>
            <dbReference type="Rhea" id="RHEA:14345"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="EC" id="4.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="3"/>
    <dbReference type="EC" id="4.1.1.25" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643984.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0381"/>
    <dbReference type="EnsemblBacteria" id="ADZ08633">
        <property type="protein sequence ID" value="ADZ08633"/>
        <property type="gene ID" value="Metbo_0381"/>
    </dbReference>
    <dbReference type="GeneID" id="10276818"/>
    <dbReference type="KEGG" id="mel:Metbo_0381"/>
    <dbReference type="eggNOG" id="arCOG00027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_2"/>
    <dbReference type="OMA" id="DPHKMGL"/>
    <dbReference type="OrthoDB" id="44488at2157"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004837">
        <property type="term" value="F:tyrosine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3 4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="392" mass="43498" checksum="1B02DDB6035C56E9" modified="2011-05-03" version="1">MEDKGRSETEIFDELHQFKTRDMTHRSGKILGSMCTCPHPIGLNAFKMFLESNLGDPGLFKGTQAMEDEVISSLGELLGERDVYGHIITGGTEANIMAMRAARNTFKHNYPDCEDVNIVVPKSAHFSFKKAADMLCLDLLEAELDENYRVDINSLDELINENTAAVVAIAGTTELGKIDPVEKISELCLKRGVYLHVDAAFGGYSIPFLNEMGYDLPNFDFSLPGVCSITIDPHKMGLAPIPTGGILFRKKTFLESISIETPYLTEDRQSTIVGTRTGASTAATWALMNYLGKEGYRKVSKECMEITELLHRGVVEAGFNPVTEPELNIVAFTSDEMTVDEIADGLERSGWAVSISSYPRAIRIIVMPHVKEEHVELLLDDLRELRSLHSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T913</accession>
    <name>F0T913_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UMP pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0383</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643986.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0383"/>
    <dbReference type="EnsemblBacteria" id="ADZ08635">
        <property type="protein sequence ID" value="ADZ08635"/>
        <property type="gene ID" value="Metbo_0383"/>
    </dbReference>
    <dbReference type="GeneID" id="10276820"/>
    <dbReference type="KEGG" id="mel:Metbo_0383"/>
    <dbReference type="eggNOG" id="arCOG04128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_0_2"/>
    <dbReference type="OMA" id="TYATRMP"/>
    <dbReference type="OrthoDB" id="85528at2157"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="23846" checksum="92F69EA23ACEA13C" modified="2011-05-03" version="1">MEIELLDHLPVQDRVTRLRRSGIKGADFRRFMTEIGMFMGYEFAKTLKTKTVNVKTPLAVTEGEIFEDESNIIVINVLRAAVPLVDGIIRIFNEAECGMIGAWREETVPFKVNVNYIKVPDINGKIVIVADPMLATGHTLKEILNEIKSRGTPARIVLFNIIASREGIDNLQKSHPEIEIYTCAIDNDVNSDGYIVPGLGDAGDISFGKPFQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T924</accession>
    <name>F0T924_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643997.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0394"/>
    <dbReference type="EnsemblBacteria" id="ADZ08646">
        <property type="protein sequence ID" value="ADZ08646"/>
        <property type="gene ID" value="Metbo_0394"/>
    </dbReference>
    <dbReference type="GeneID" id="10276831"/>
    <dbReference type="KEGG" id="mel:Metbo_0394"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="OMA" id="FEMDSRA"/>
    <dbReference type="OrthoDB" id="99888at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13106" checksum="D3155E819F29899B" modified="2011-05-03" version="1">MAKTKKELLDDVLAGILQVGQIKACGVVSKEGLLIDSRTPPDVDARIFSALCSTIMGAAEAASGQLNTGSVDQISVKTEKGTIVLLPAGSKAILTALTEPEAQLGLILFEMESRALQVNEILDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T959</accession>
    <name>F0T959_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644032.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0429"/>
    <dbReference type="EnsemblBacteria" id="ADZ08681">
        <property type="protein sequence ID" value="ADZ08681"/>
        <property type="gene ID" value="Metbo_0429"/>
    </dbReference>
    <dbReference type="GeneID" id="10276866"/>
    <dbReference type="KEGG" id="mel:Metbo_0429"/>
    <dbReference type="eggNOG" id="arCOG04407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_2"/>
    <dbReference type="OMA" id="GIGRTHF"/>
    <dbReference type="OrthoDB" id="56592at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="285" mass="31123" checksum="21A280C664CB5BBB" modified="2011-05-03" version="1">MDLINLVKDAVIDASTNFREDQLTAYKNAVKTETNPNARWVLELLLKNAEIASTNKVPLCDDTGVPHVLIEVGREVNLAPGFFEDITKGIEAGLKELPARPMAVKGNSIERIEQSQGLYNQPEMLPAPPFSVTGIKGDVVKIHVLMLGGGPEIRAHTYKVFHKRDHRKVFLEVLTWLRSEIAMLGCTPCIPAIGIGRTHFEASSLMLKAMAYGNLNIQSELENCMTGKLNNTNIGSLGIGGSVTALGSFIEVGPQRASGVRVVSMRPCCCVEPRRSTVQLSKFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T972</accession>
    <name>F0T972_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein Metbo_0442</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644045.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0442"/>
    <dbReference type="EnsemblBacteria" id="ADZ08694">
        <property type="protein sequence ID" value="ADZ08694"/>
        <property type="gene ID" value="Metbo_0442"/>
    </dbReference>
    <dbReference type="GeneID" id="10276879"/>
    <dbReference type="KEGG" id="mel:Metbo_0442"/>
    <dbReference type="eggNOG" id="arCOG01921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146474_0_0_2"/>
    <dbReference type="OMA" id="LHNLKSN"/>
    <dbReference type="OrthoDB" id="112654at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="15767" checksum="FF03660063C3E48C" modified="2011-05-03" version="1">MTTDFSRGDKLVKKQILILNKHLPRKRKFLEELLKEDKPHVIGADGTRHRFKREELERISGILGKSNFNKLKLPIYIEIDSNASGSRIAGKYESEVVCKILEIDNCETEMYIYRADLKKLRSILPTTTQYIFLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C3</accession>
    <name>F0T9C3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1648</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645225.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1648"/>
    <dbReference type="EnsemblBacteria" id="ADZ09874">
        <property type="protein sequence ID" value="ADZ09874"/>
        <property type="gene ID" value="Metbo_1648"/>
    </dbReference>
    <dbReference type="GeneID" id="10278105"/>
    <dbReference type="KEGG" id="mel:Metbo_1648"/>
    <dbReference type="HOGENOM" id="CLU_2893265_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="62" mass="7555" checksum="772540C87981CDBA" modified="2011-05-03" version="1">MWGQHKEHMMGGHDMMGEKMMEMMSKEDMMAMYAMKMDEKIEMLELKLKYLKKSREMLKSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D7</accession>
    <name>F0T9D7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aliphatic sulfonates family ABC transporter, periplasmic ligand-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645239.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1662"/>
    <dbReference type="EnsemblBacteria" id="ADZ09888">
        <property type="protein sequence ID" value="ADZ09888"/>
        <property type="gene ID" value="Metbo_1662"/>
    </dbReference>
    <dbReference type="GeneID" id="10278119"/>
    <dbReference type="KEGG" id="mel:Metbo_1662"/>
    <dbReference type="eggNOG" id="arCOG01803">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_10_4_2"/>
    <dbReference type="OMA" id="PEGMWEN"/>
    <dbReference type="OrthoDB" id="78272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="318" mass="34446" checksum="B5875DA7EAD506EA" modified="2011-05-03" version="1" precursor="true">MNQKRTVLIVLGVLIVVGLALTTTYSSSDTNKIKVAYIPCDHDAALFIALAQNKYSANGLNVEPTSISTGSSIVSALASGDIDVGYIGIAPALQGIAEGVPIKIVGAVNEDGSGIVVNTNSDINNTTDLVGKKIATPGVSSIQQVLLLYQLAKYNITKNQVDISSVNIFMLPSTLASDKVDGYIAYEPYVSIAPFTNVGRVLMYSDEIMPNHPCCVIVARQDFIDQHPQELQTFLDIHKNCTEFANNNTNQTASLVSDELTTNPELEKTALKHVIFVSQLNNDFQTKVLDFMNIEIEMGYLKKNLTSSQIFDSSFLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D9</accession>
    <name>F0T9D9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645241.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1664"/>
    <dbReference type="EnsemblBacteria" id="ADZ09890">
        <property type="protein sequence ID" value="ADZ09890"/>
        <property type="gene ID" value="Metbo_1664"/>
    </dbReference>
    <dbReference type="GeneID" id="10278121"/>
    <dbReference type="KEGG" id="mel:Metbo_1664"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011907_4_2_2"/>
    <dbReference type="OMA" id="KSQQHRW"/>
    <dbReference type="OrthoDB" id="48346at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="190"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="537" mass="61898" checksum="1A726567B1DA399A" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K4</accession>
    <name>F0T9K4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0500"/>
    <dbReference type="EnsemblBacteria" id="ADZ08752">
        <property type="protein sequence ID" value="ADZ08752"/>
        <property type="gene ID" value="Metbo_0500"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0500"/>
    <dbReference type="HOGENOM" id="CLU_3227812_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="5062" checksum="947D3FDADFBFD3AE" modified="2011-05-03" version="1">MVYISDLDHHFLIMELKLINVVNSISTFTLSFEAIYLAKERDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P5</accession>
    <name>F0T9P5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645275.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1699"/>
    <dbReference type="EnsemblBacteria" id="ADZ09924">
        <property type="protein sequence ID" value="ADZ09924"/>
        <property type="gene ID" value="Metbo_1699"/>
    </dbReference>
    <dbReference type="GeneID" id="10278156"/>
    <dbReference type="KEGG" id="mel:Metbo_1699"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745579_0_0_2"/>
    <dbReference type="OMA" id="HRETAQK"/>
    <dbReference type="OrthoDB" id="107064at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="17578" checksum="2E7E5207415E781E" modified="2011-05-03" version="1">MEDINEIVRSWEQINKQIRLKHRETAQKYGFSFEQFHLLIELDHHNEMTISSDSLPPTIGNIADFTGNAPHTISERVKRLENRGILLKIKDEKDHRICRVMLSKEGQKMIDTIKNEARNIFLNNALNKMDDKSLKNLLKSLKELNKNLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T8</accession>
    <name>F0T9T8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645318.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1744"/>
    <dbReference type="EnsemblBacteria" id="ADZ09967">
        <property type="protein sequence ID" value="ADZ09967"/>
        <property type="gene ID" value="Metbo_1744"/>
    </dbReference>
    <dbReference type="GeneID" id="10278201"/>
    <dbReference type="KEGG" id="mel:Metbo_1744"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OMA" id="CFGHGDP"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="24834" checksum="5942AF0DFE9EF716" modified="2011-05-03" version="1">MGKSKFTEVITGIYQFQGISNFYLVQDSESFVVDTGLPGKAGQLENCLNTLGKNPEDIGVIVLTHHHFDHTGSLNKIEQLTKAKIAAHKDDWPYVSGEKGDDGPFIMKPFTKLMNLIYNIKPVKTDMILEDGDKIANYTVIHTPGHTPGSICLYNPINKVLFVGDNMSYSNGVLNSPSGRLLPDPVNYKKSMEKLGKLDVETILTGHGEAVTTDANKLIEEFINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W7</accession>
    <name>F0T9W7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease P protein component 4</fullName>
            <shortName evidence="2">RNase P component 4</shortName>
            <ecNumber evidence="2">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnp4</name>
        <name evidence="3" type="ordered locus">Metbo_0538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644141.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0538"/>
    <dbReference type="EnsemblBacteria" id="ADZ08790">
        <property type="protein sequence ID" value="ADZ08790"/>
        <property type="gene ID" value="Metbo_0538"/>
    </dbReference>
    <dbReference type="GeneID" id="10276983"/>
    <dbReference type="KEGG" id="mel:Metbo_0538"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_3_0_2"/>
    <dbReference type="OMA" id="HVVITCL"/>
    <dbReference type="OrthoDB" id="128960at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="16021" checksum="AC7D518E9D237FF8" modified="2011-05-03" version="1">MRRGRRPKWILKIAEERINILFSRADEEFNQHPERSHRYVEMARNIAKKYNLKLPDKWNRRFCHSCHQFLKPGLNCRVRLINSSVVIKCLDCGHVARIPYITEKKERRRRKIEHYNASKKGTDEQITFNHHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X5</accession>
    <name>F0T9X5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L18Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl18a</name>
        <name evidence="3" type="synonym">rpl20e</name>
        <name evidence="3" type="synonym">rplX</name>
        <name evidence="5" type="ordered locus">Metbo_0546</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644149.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0546"/>
    <dbReference type="EnsemblBacteria" id="ADZ08798">
        <property type="protein sequence ID" value="ADZ08798"/>
        <property type="gene ID" value="Metbo_0546"/>
    </dbReference>
    <dbReference type="GeneID" id="10276991"/>
    <dbReference type="KEGG" id="mel:Metbo_0546"/>
    <dbReference type="eggNOG" id="arCOG04175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177460_0_1_2"/>
    <dbReference type="OMA" id="MVFTKEY"/>
    <dbReference type="OrthoDB" id="125208at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_L18Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="50S_L18Ae/Ribosomal_L18a/L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_L18a//L18Ae/LX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18A" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="76" mass="8827" checksum="63BF0D9827F619F4" modified="2011-05-03" version="1">MKTKVFKIQGKFMMGQGLKNFTKELKATSEDDIKEKIYSEFGSKHRIGRNKIFIDEIKEISEEEAQDPMIKALSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA65</accession>
    <name>F0TA65_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase C39 bacteriocin processing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10015">
        <property type="protein sequence ID" value="ADZ10015"/>
        <property type="gene ID" value="Metbo_1793"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1793"/>
    <dbReference type="eggNOG" id="arCOG06761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182870_0_0_2"/>
    <dbReference type="OMA" id="NASSHYM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C39" evidence="1">
        <location>
            <begin position="61"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="27567" checksum="871B891833E634FB" modified="2011-05-03" version="1" precursor="true">MNLKLKLILMVLSLVIVAVPLGVYSESTNNSTENIPIINITNSQNLSHNNTTILRVPDLLAPTANSTGPTALQEVLAYYGTNIGIDKLVNLTNNTENGTFPNNIVEAADSLGFQGEIKENMSLEDLQNYVDQGIPVIVNIQAGINGTENFNWTSSQQNGEYMVIVGVDSQNVYLEDPTILGSVGYIPNQEFLDRWHATFQTGTNSTNSSTNSTNTTNNTNSTNSTNFNILFYNHMGIIVTGGSSTSPPLFVKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD6</accession>
    <name>F0TAD6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Replication factor C small subunit</fullName>
            <shortName evidence="6">RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rfcS</name>
        <name evidence="8" type="ordered locus">Metbo_0637</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0637"/>
    <dbReference type="EnsemblBacteria" id="ADZ08888">
        <property type="protein sequence ID" value="ADZ08888"/>
        <property type="gene ID" value="Metbo_0637"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0637"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_0_2"/>
    <dbReference type="OMA" id="SCNYSSQ"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:DNA replication"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
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        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="7">
        <location>
            <begin position="61"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="347" mass="39198" checksum="81A43B7409C68A52" modified="2011-05-03" version="1">MKVDLDKNYIRQQLINNATQYENGDSMNAPWVEKYRPQTLDEVVGQEHTILRLKRYVKEGNMPNLMFTGPAGVGKTTTSIALAKEMLGEYWRQNFLELNASDARGIDTVRNDIKSFCRLKAVGSPFRIIFLDEVDNMTKDAQHALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFVPVKGHQIIKRLEYIAQAEGLKIDIAAIESIVYFAEGDMRRAVNILQASSSAGEEVTEESVDEVVSKAKPKDVKKIVNKALDGDFIGARELLRDVMVVQGTSGEDMVTQIYQEVSKMALDGSLNEDIYINLVESIGETDYRIREGSNPRIQLEALLTKFLINSKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE0</accession>
    <name>F0TAE0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644243.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0641"/>
    <dbReference type="EnsemblBacteria" id="ADZ08892">
        <property type="protein sequence ID" value="ADZ08892"/>
        <property type="gene ID" value="Metbo_0641"/>
    </dbReference>
    <dbReference type="GeneID" id="10277086"/>
    <dbReference type="KEGG" id="mel:Metbo_0641"/>
    <dbReference type="eggNOG" id="arCOG07448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709142_0_0_2"/>
    <dbReference type="OMA" id="YLVFRDC"/>
    <dbReference type="OrthoDB" id="65838at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="188" mass="21007" checksum="E5FA43DCEC5CA677" modified="2011-05-03" version="1">MAYEIKREGKEIFGDFKPELIPDECGGDIDDYEEALHVLMKFVGSMSSALEQVSGRGANAIVYQAGKRMGHEAGKMVEKTDNLEKAMQELSEILGAEFYFDMWKPAGQEGYTIEKGDETVVKLIFRDCVVRQTLRRTGLPQKGPLCYLLYGYTVGAVEEVMDIRGKLDIDHVGPNACLKTLTIKWSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG0</accession>
    <name>F0TAG0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644263.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0661"/>
    <dbReference type="EnsemblBacteria" id="ADZ08912">
        <property type="protein sequence ID" value="ADZ08912"/>
        <property type="gene ID" value="Metbo_0661"/>
    </dbReference>
    <dbReference type="GeneID" id="10277106"/>
    <dbReference type="KEGG" id="mel:Metbo_0661"/>
    <dbReference type="eggNOG" id="arCOG00405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_1_2_2"/>
    <dbReference type="OMA" id="EPSYGID"/>
    <dbReference type="OrthoDB" id="32623at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="3"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="575" mass="65337" checksum="6D8EB270618C5A17" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI2</accession>
    <name>F0TAI2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645405.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10054">
        <property type="protein sequence ID" value="ADZ10054"/>
        <property type="gene ID" value="Metbo_1832"/>
    </dbReference>
    <dbReference type="GeneID" id="10278289"/>
    <dbReference type="KEGG" id="mel:Metbo_1832"/>
    <dbReference type="HOGENOM" id="CLU_2204135_0_0_2"/>
    <dbReference type="OrthoDB" id="139903at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="12621" checksum="C6ABE30F0370647E" modified="2011-05-03" version="1">MQIDNVKINREKNAFHGELNFFDNELSKDDSKEMLYRLLSWKLGPVEPTRHFELSYNSDKNRVEVDVFVSVTKAEAGNVDTVKYIFSKLMEFTAIYNREFEDLSKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP2</accession>
    <name>F0TAP2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645465.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10114">
        <property type="protein sequence ID" value="ADZ10114"/>
        <property type="gene ID" value="Metbo_1894"/>
    </dbReference>
    <dbReference type="GeneID" id="10278352"/>
    <dbReference type="KEGG" id="mel:Metbo_1894"/>
    <dbReference type="eggNOG" id="arCOG02240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_0_1_2"/>
    <dbReference type="OMA" id="TVIEINM"/>
    <dbReference type="OrthoDB" id="107985at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037479">
        <property type="entry name" value="Tauto_MSAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38460">
        <property type="entry name" value="PTHR38460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14552">
        <property type="entry name" value="Tautomerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="117" mass="13300" checksum="E966EC660A8CECD1" modified="2011-05-03" version="1">MPMVKIEVLEGKTSDYKKSVLNSIKNAIKDAVAVDEIKIVQRLYEISEETCTIPSDRSKNLTIIEITLYPGRTESTKKKLFKEIVKNLSEDPGIEAGDIITVLLEPPMENWLFKYPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ2</accession>
    <name>F0TAQ2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="11">ASA dehydrogenase</shortName>
            <shortName evidence="11">ASADH</shortName>
            <ecNumber evidence="7 11">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">asd</name>
        <name evidence="13" type="ordered locus">Metbo_1904</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645475.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1904"/>
    <dbReference type="EnsemblBacteria" id="ADZ10124">
        <property type="protein sequence ID" value="ADZ10124"/>
        <property type="gene ID" value="Metbo_1904"/>
    </dbReference>
    <dbReference type="GeneID" id="10278362"/>
    <dbReference type="KEGG" id="mel:Metbo_1904"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OMA" id="WPEMVDN"/>
    <dbReference type="OrthoDB" id="26718at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="11 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="12">
        <location>
            <begin position="3"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="11">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="348" mass="38073" checksum="769B542473AFC2CC" modified="2011-05-03" version="1">MVNVGILGATGMVGQRFIQLLADHPDFEVTALTASSRSAGKKYEDAVTWHLETKIPDAVKDTVVVNTDPREVKDVEIVFSALPSQNAAKVEPKFAEAGMKVASNASAMRMEPDVPLVVPEVNPEHLDLIETQQKRRGWDGFIVTNPNCSTIALVMTLKPLYDQFNIKRVYVSTMQAVSGAGYNGVPSMAIVDNLVPFIGEEEEKMESETLHLLGDFDGETVNNANFGVSASCHRVAVLDGHTEAVFIEMDDDFDLDEVKGSLTNFKGLPQKLNLYSAPEDPVVIREEDNRPQPRMDRMAGNGMAVSVGRLRRDEVYSNSLKYVLVGHNTIRGAAGASILNAELINEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS4</accession>
    <name>F0TAS4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Queuine/other tRNA-ribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644301.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0699"/>
    <dbReference type="EnsemblBacteria" id="ADZ08950">
        <property type="protein sequence ID" value="ADZ08950"/>
        <property type="gene ID" value="Metbo_0699"/>
    </dbReference>
    <dbReference type="GeneID" id="10277144"/>
    <dbReference type="KEGG" id="mel:Metbo_0699"/>
    <dbReference type="eggNOG" id="arCOG00997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131609_0_0_2"/>
    <dbReference type="OMA" id="VIHGSKY"/>
    <dbReference type="OrthoDB" id="61658at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TGT" evidence="1">
        <location>
            <begin position="19"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="27978" checksum="79C88DFF93589A15" modified="2011-05-03" version="1">MIEIKLHDGPARLGKYIKMDTPNLIKSDNSLKILKDEPMPYNVPESLAEWSVNSTLEYARNSEETGIGVVHGSKYSDLRIKCALELEKLGNSILMIANPESLIENSRDLVEIIAKLREYINPNTGIYFPFAKPSYMPFLTYLGVDFFDNVAADYYAELNTLLTSTQQYNLDVYKLYDMDLEELTNYNRNTIEFTLKEIQEHIKNGTLRNLVEERSCSSPELMTALRLLDRDHSDFVSSYTQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT7</accession>
    <name>F0TAT7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644314.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0712"/>
    <dbReference type="EnsemblBacteria" id="ADZ08963">
        <property type="protein sequence ID" value="ADZ08963"/>
        <property type="gene ID" value="Metbo_0712"/>
    </dbReference>
    <dbReference type="GeneID" id="10277159"/>
    <dbReference type="KEGG" id="mel:Metbo_0712"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081793_0_0_2"/>
    <dbReference type="OMA" id="SPCGICI"/>
    <dbReference type="OrthoDB" id="45763at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="31411" checksum="B743CC429D5A472A" modified="2011-05-03" version="1">MKSKPINERIIDKIKELGIPIFGFAPVDRWVKPPLDSPNKFRDWIPEEFWPQSIFPGTQTVIVIGLPVELPIVETAPSIHYHEHYSTVNHVLDAKAYEISCFLNSMGHPSVSIPRDGYGSIEVLLEKPLAFFSHKYAAYIAGLGTLGENNVLLTREYGPRVRFTSIFCSLEVEGDPILTEDLCIHCKSCVHHCPVDAIESMDDDCMKIMDKLKCATRSKKLRSEFRSPCGICIKVCPIGEDREHYKRTDTSIYDQSNHKYHSGWEHVRSYGSLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV2</accession>
    <name>F0TAV2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644329.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0727"/>
    <dbReference type="EnsemblBacteria" id="ADZ08978">
        <property type="protein sequence ID" value="ADZ08978"/>
        <property type="gene ID" value="Metbo_0727"/>
    </dbReference>
    <dbReference type="GeneID" id="10277174"/>
    <dbReference type="KEGG" id="mel:Metbo_0727"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_0_0_2"/>
    <dbReference type="OMA" id="TAYIHGF"/>
    <dbReference type="OrthoDB" id="96698at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35528" checksum="B63989126FCA050E" modified="2011-05-03" version="1">MKISVIVPMFNEELNVQRTLSEINNALNDYSDFEIIAVDDGSTDKTFSLLNEFRAKNSHIIVLKHKTNYGMGKAIRTGFEESDGDIIITIDADLSYRALNIPVLVSELENDISIDIVVGSQYMEGGDVKNVPFNRLFISKVANKFIGYSMTENLNTVTGVLRAYRREVLESMEIESNGTKINLEILSKAIATGFKIKEVPVVLEGRELGESKIKIKSKTISHVLFSFYEKPMILFGVIGLFMLLIGIISGIYLFYQYFLGTLDPTRPLMIFMVLMIISGIQILIFGFIATQISLLKREIFIVQKENKLLRKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ2</accession>
    <name>F0TAZ2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645489.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1918"/>
    <dbReference type="EnsemblBacteria" id="ADZ10138">
        <property type="protein sequence ID" value="ADZ10138"/>
        <property type="gene ID" value="Metbo_1918"/>
    </dbReference>
    <dbReference type="GeneID" id="10278376"/>
    <dbReference type="KEGG" id="mel:Metbo_1918"/>
    <dbReference type="HOGENOM" id="CLU_2461790_0_0_2"/>
    <dbReference type="OrthoDB" id="142997at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="88" mass="10627" checksum="51D43EFBB0883BA2" modified="2011-05-03" version="1">MIKLKKSPQINTKTDAMKEIEAKLSSSEIVKEDQVTEVEEWTDDPKRWRLFKERYMDEFEDKLDLMDNLMTQKRGKVEVTWISIFKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB03</accession>
    <name>F0TB03_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645500.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1929"/>
    <dbReference type="EnsemblBacteria" id="ADZ10149">
        <property type="protein sequence ID" value="ADZ10149"/>
        <property type="gene ID" value="Metbo_1929"/>
    </dbReference>
    <dbReference type="GeneID" id="10278387"/>
    <dbReference type="KEGG" id="mel:Metbo_1929"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118417_0_0_2"/>
    <dbReference type="OMA" id="GNLEQWY"/>
    <dbReference type="OrthoDB" id="94672at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="199" mass="23364" checksum="93D5C522D13C93CF" modified="2011-05-03" version="1">MSMKSAVKEHVNSHVTEHVKKHGSFLLNEILLDGFISKLEVDNQNFVKYMDGLEFKKFSQEDLDNSAKLYQEVFSQQPWNDEWFRLSRVKEYLRELIENPVFSGFVVYQNSELVAACLGHSRSWWSGDEFFIDEFFVSSKYQGQGIGSLLMDYVENHPELKNIESFQLSTNSNVPAKDFYHKNGFKTRKNRVTMTKKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB60</accession>
    <name>F0TB60_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxal phosphate enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644362.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0760"/>
    <dbReference type="EnsemblBacteria" id="ADZ09011">
        <property type="protein sequence ID" value="ADZ09011"/>
        <property type="gene ID" value="Metbo_0760"/>
    </dbReference>
    <dbReference type="GeneID" id="10277208"/>
    <dbReference type="KEGG" id="mel:Metbo_0760"/>
    <dbReference type="eggNOG" id="arCOG00114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055443_0_0_2"/>
    <dbReference type="OMA" id="RIDFCSK"/>
    <dbReference type="OrthoDB" id="37272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020033">
        <property type="entry name" value="PyrdxlP-dep_transferase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03576">
        <property type="entry name" value="pyridox_MJ0158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="386" mass="42053" checksum="2575965075218685" modified="2011-05-03" version="1">MLTNSIIDEVNRREAALRVIKSILQTKGRKGFYDLTGLAGGFPILSEDKALLETYAGPAVFDDAIQTVGKIHLGGDKVLALNRTSSGILASVLAIVKPGDEVAHYLPKSPAHPSIPRSAELVGASYIEFDKIEEFKVEDNTSLVFITGSTMDHEIITQEDFRRVIEICKAKNIPVAVDDASGARLRTIVYRQPRAIDMGADIAITSTDKLMDGPRGGLMSGKGEIMQLVKSKAHQFGLEAQAPLVAAMVRALENLSPEKILMAFSAKHELKEALNNQFSMVKETPTGIMLTPEAIEFELRQIETETDLESDDLAYLFAMILLRDHGIITIPAVGMPGASAAIRIDLAANDAERIGSKEIAEAFKKTFTKLQSVANNEEESKKILYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB74</accession>
    <name>F0TB74_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl3</name>
        <name evidence="8" type="ordered locus">Metbo_0774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644376.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0774"/>
    <dbReference type="EnsemblBacteria" id="ADZ09025">
        <property type="protein sequence ID" value="ADZ09025"/>
        <property type="gene ID" value="Metbo_0774"/>
    </dbReference>
    <dbReference type="GeneID" id="10277222"/>
    <dbReference type="KEGG" id="mel:Metbo_0774"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="OMA" id="HQRTEYN"/>
    <dbReference type="OrthoDB" id="31722at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_L3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_L3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="PTHR11363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="337" mass="37299" checksum="2F54B319F9963BA5" modified="2011-05-03" version="1">MTRHHQPRSGSMAFSPRKRAAKETPKVRSWPETEESCLLGFPGYKVGMTHVTMLDNVKNSPTSGMEVSSPVTILETPPVVVMGVRAYRKDTRGLKTLNDVMAKDLDEDLTRKITLPKDYDVEAKLDEMKEQLDEVEDIRVLLHTKPRLASGIPKKKPEIIECGIGGKSVEDKFEFARSLLGKEVNPADSFSDGEHVDTIAVTKGKGFQGPVKRFGVRIQYGKAARSSKARHVGSIGPWTPARTMWTVAMAGQMGYHKRTEFNKKILRIADESQVDEVNPKGGFVKYGLIKNGYVMIKGSIPGPSKRLVILRKAVRPIGKHNDAPEITHISTASKQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB87</accession>
    <name>F0TB87_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">30S ribosomal protein S4e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="9" type="ordered locus">Metbo_0787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644389.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0787"/>
    <dbReference type="EnsemblBacteria" id="ADZ09038">
        <property type="protein sequence ID" value="ADZ09038"/>
        <property type="gene ID" value="Metbo_0787"/>
    </dbReference>
    <dbReference type="GeneID" id="10277235"/>
    <dbReference type="KEGG" id="mel:Metbo_0787"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OMA" id="FPVGLMD"/>
    <dbReference type="OrthoDB" id="89943at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="KOW_RPS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_S4e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_S4e_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_S4e_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_S4e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="PTHR11581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S4" evidence="8">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="26994" checksum="83253AA86504851C" modified="2011-05-03" version="1">MAKMGSRKHLKRYKSPAHWPIHPKEDKWTVKPSAGPHAIEESLPLLIVVRDILKVADNSREAKIIINNGDILVDGRARKDYKYPVGFMDVIELPKSEKVYRVLPDHKGRLILHPISEKNREFKLCRVTDKTIIKGGKTQLNLHDGRNCIVEKQYSTGDVVVVEIPEQKVTDHIKFESGTVGLIIGGKHIGELGKVKQINITQSSKPNTVEVETDEGIFLTLADYVFVLGKEEPVISLPGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE8</accession>
    <name>F0TBE8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1998"/>
    <dbReference type="EnsemblBacteria" id="ADZ10217">
        <property type="protein sequence ID" value="ADZ10217"/>
        <property type="gene ID" value="Metbo_1998"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1998"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833169_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="226"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="36926" checksum="4538D32ED951F98F" modified="2011-05-03" version="1" precursor="true">MIVRGEKNMKKGFLVAAVLCCVFAFGSMTAISAAPTGTYLANIGSYTAVGTGPWNTVGFTLNSPQAETLQFEWKSEIVYEKYHVQLETIYTKTSGIREIVTDDMDIMLMGYYNGKLLQTPDIDLTKNVYVTVTTWRINSGKQNITPGAPVVKADVNTGLYNTNKLVTLSINKPGTIYYTLNGKIPTNTSNKYSKPIKITSTTILNYMAIGTQLDKSPLYSKLYIIDKIPPRVTAAKPTNNSKHFSLTSPIKIYYNEKISKGTNYSKIYIKNLSTGKIAKSTVTSISGNVLTLKMTKSRLSQNTYQVYIPTNAIKDTAGNKNAKYTLKFKTSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ1</accession>
    <name>F0TBJ1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Adenylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645611.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2041"/>
    <dbReference type="EnsemblBacteria" id="ADZ10260">
        <property type="protein sequence ID" value="ADZ10260"/>
        <property type="gene ID" value="Metbo_2041"/>
    </dbReference>
    <dbReference type="GeneID" id="10278502"/>
    <dbReference type="KEGG" id="mel:Metbo_2041"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OMA" id="VNYGELM"/>
    <dbReference type="OrthoDB" id="109827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="20535" checksum="8E31FE8F65525AF4" modified="2011-05-03" version="1">MNWNITALVGVPGVGKTSLCKTATGQVGIKYVNYGELMLEVATSNDLASSQGEMFHLKLEVQEFIWKQAAIQVRTMAKNQNLLVDLHGLDISDEGYIQSLPIETFCPDLIVIVEASYEDIMLRRMGDDTRNRSLVDLKSLEEQFQLLRMSMMSAAVLCSAFVKILHNDDLDSCVTELVSLLSKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ5</accession>
    <name>F0TBJ5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="4">PRAI</shortName>
            <ecNumber evidence="4">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trpF</name>
        <name evidence="6" type="ordered locus">Metbo_2045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645615.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2045"/>
    <dbReference type="EnsemblBacteria" id="ADZ10264">
        <property type="protein sequence ID" value="ADZ10264"/>
        <property type="gene ID" value="Metbo_2045"/>
    </dbReference>
    <dbReference type="GeneID" id="10278507"/>
    <dbReference type="KEGG" id="mel:Metbo_2045"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_2_0_2"/>
    <dbReference type="OMA" id="DCLDFNS"/>
    <dbReference type="OrthoDB" id="117266at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PRAI" evidence="5">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="235" mass="26400" checksum="D8D274E5E6D77AE0" modified="2011-05-03" version="1">MKIKVCGITRSEDLTRCEESGAELMGFINVKRSKRNLSLKEIKNLRSGMKNKEKAVLVLEPADIEEVVMKMKKTGVRNVQLHSLTPNQINYLRWIEKYERTPFEAHLKIFRAVGLSPDSTKIKNGETVLTPGKKREIEEFARCCDALVLDYQMNGKSGGTGKQIPMKIALEAVETAKNADNNVEIFLAGGINTAIIKNRKEVLEKVFDYVDVNSGVEDAPGIKNSQQLKELFELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS6</accession>
    <name>F0TBS6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644504.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0904"/>
    <dbReference type="EnsemblBacteria" id="ADZ09153">
        <property type="protein sequence ID" value="ADZ09153"/>
        <property type="gene ID" value="Metbo_0904"/>
    </dbReference>
    <dbReference type="GeneID" id="10277353"/>
    <dbReference type="KEGG" id="mel:Metbo_0904"/>
    <dbReference type="eggNOG" id="arCOG04188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_2"/>
    <dbReference type="OMA" id="RGVFLEW"/>
    <dbReference type="OrthoDB" id="96034at2157"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="21285" checksum="80CD095F00767D7A" modified="2011-05-03" version="1">MGKFKFIETSIDDVYIIEPTVFGDDRGYFMETYHQGEFKEAGLDMEFVQDNQSRSVRGVLRGLHFQYTQPQGKLVRVIKGTVFDVAVDLRENSPTYGQYESVVLSEENKKQFYVPEGFAHGFLVLSDVAEFTYKCTAFYNADDEGGIMWNDPDINIEWPLKEAGNVVLSDKDSEWKPLKETNVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS7</accession>
    <name>F0TBS7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644505.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0905"/>
    <dbReference type="EnsemblBacteria" id="ADZ09154">
        <property type="protein sequence ID" value="ADZ09154"/>
        <property type="gene ID" value="Metbo_0905"/>
    </dbReference>
    <dbReference type="GeneID" id="25394718"/>
    <dbReference type="KEGG" id="mel:Metbo_0905"/>
    <dbReference type="eggNOG" id="arCOG01384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011795_0_0_2"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="511"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="745"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1008" mass="117118" checksum="F5B6EDD651AB258C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT9</accession>
    <name>F0TBT9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645632.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2062"/>
    <dbReference type="EnsemblBacteria" id="ADZ10281">
        <property type="protein sequence ID" value="ADZ10281"/>
        <property type="gene ID" value="Metbo_2062"/>
    </dbReference>
    <dbReference type="GeneID" id="24964582"/>
    <dbReference type="KEGG" id="mel:Metbo_2062"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718893_0_0_2"/>
    <dbReference type="OrthoDB" id="124249at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DUF11" evidence="2">
        <location>
            <begin position="52"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="3">
        <location>
            <begin position="278"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="384" mass="41896" checksum="F73485FF1FC0DC2B" modified="2011-05-03" version="1" precursor="true">MTNINKIPLIFLFIIIFSCISAVNAENPQQVQNSPTVTYPADPTMLYSSSVSIATSENTAHAGDTVQILVTITNTGLVNWNNVSLYIPIPNGTQFVSNVVLDRNQNLQNYNPLTGVWDLNIITHIERGQQKTLCLTVKVLPEAVGKTLSVMAKFNQLLLEGSGIDMSNQAPSARSVSLVVLNGGNCSGNGSMGNGGGTSFSNPWANTSSGIYNKNQLVKLQVNGNETIYYTTNGKTPNNMSQKYTKPISINSTTLLKFIAMDQFSDKSTVYTKNYIIDKVPPKVVKSTPKNNQVGFSLSAPLTLTFNEKISKASQYNNIQVKNKNTGKLVTIKKSVSGNKLTIKMVRSRLSLNNYQIYIPTGAFKDAAGNKNSKYLSTFKTGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ1</accession>
    <name>F0TBZ1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">Metbo_2114</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645684.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2114"/>
    <dbReference type="EnsemblBacteria" id="ADZ10333">
        <property type="protein sequence ID" value="ADZ10333"/>
        <property type="gene ID" value="Metbo_2114"/>
    </dbReference>
    <dbReference type="GeneID" id="10278577"/>
    <dbReference type="KEGG" id="mel:Metbo_2114"/>
    <dbReference type="eggNOG" id="arCOG04421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_2"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="OrthoDB" id="47738at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="9"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="248" mass="28248" checksum="0DD5B40E5F76E1C2" modified="2011-05-03" version="1">MNPKMGNLIYAGKAKTVYETDDPDQVIVKFRDDITAGDGVKTESLVRKGYYNSVISAKFFEVLEDAGIKTQYIKLIKPGYMLCKKLDMIPLEVITRNIAAGSLLKKFPFTEKQELEPPIIQMDYKNDEYHDPMLNDSITIALELATKEELDTIRSITLEINSVLKEFLLSKAILFPDFKIEYGFDSEENIVLGDEISPDTCRLWDAQTSEVLDKDLFRKGEDGVMDAYMKVASLLLDETDIAKWNVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ8</accession>
    <name>F0TBZ8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein Crp</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645691.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2121"/>
    <dbReference type="EnsemblBacteria" id="ADZ10340">
        <property type="protein sequence ID" value="ADZ10340"/>
        <property type="gene ID" value="Metbo_2121"/>
    </dbReference>
    <dbReference type="GeneID" id="10278584"/>
    <dbReference type="KEGG" id="mel:Metbo_2121"/>
    <dbReference type="eggNOG" id="arCOG04399">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090237_0_0_2"/>
    <dbReference type="OMA" id="PHIKQKE"/>
    <dbReference type="OrthoDB" id="41689at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="28852" checksum="0918ACE79DFCF82E" modified="2011-05-03" version="1">MLVFKKKGELTKFQILAEIAKEQPHLRQKDIANELGITIQAVSENIKSLVDEGYVETGMGNAKYNITKRGIEKVKTDALNLRKYADNVLETMNTYKSVWPAIAREDMAKGETVWLEMENGTLYAGKKKKSAYAEVLKDAVAGDDVALIKLGGTIKLKPGKITIIQVPTINEGGSNACSLEKIKKIYNGNFDKVGIMGTVSRVVINKAGIESDFEFATPNATVAASKRGLNVLVFTVGKMSKSLIRKLDEEGLKYTLEDVSTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC38</accession>
    <name>F0TC38_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ordered locus">Metbo_0942</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644540.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0942"/>
    <dbReference type="EnsemblBacteria" id="ADZ09189">
        <property type="protein sequence ID" value="ADZ09189"/>
        <property type="gene ID" value="Metbo_0942"/>
    </dbReference>
    <dbReference type="GeneID" id="10277391"/>
    <dbReference type="KEGG" id="mel:Metbo_0942"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="OrthoDB" id="80060at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="420" mass="45149" checksum="E8269BD744174F45" modified="2011-05-03" version="1">MDLTVQAANEITGTVKAPPSKSYSHRAFLMAFLADGESTLRDPLYSEDTLASLNTGIEMGSTVKRTESLCSIQGVAGKPKTPYDVLDLKNSGTTLRIMTSLASLADGFTVLTGDNSLKTRPMQDLLDALKPLGVTACSTRNDGKAPIIVKGGFYGGETSIKGDVSSQFISSIIMAAPLAKKPVDLKVKGEFISQPYVMMTLELMNKFGVKVDFDKTENSFHIEPQSYKPADYTVEGDYSSASYIIAAAAALKSDVKIKNLMKDSKQGDKIILDIVRDMGCEVKVKNNEVEIKGQGMLNGVDVNLQNAPDLLPTVAALGAMADGTTNIYGVEHARFKETDRIHTCSTELSKLGVSLIEKRDGIQINGGVHGGVVKSHMDHRLAMAFYIVGLKVGGICIKDASVYKVSFPDFPDIMKKLTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC48</accession>
    <name>F0TC48_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ordered locus">Metbo_0952</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644550.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0952"/>
    <dbReference type="EnsemblBacteria" id="ADZ09199">
        <property type="protein sequence ID" value="ADZ09199"/>
        <property type="gene ID" value="Metbo_0952"/>
    </dbReference>
    <dbReference type="GeneID" id="10277401"/>
    <dbReference type="KEGG" id="mel:Metbo_0952"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OMA" id="CPNRNCI"/>
    <dbReference type="OrthoDB" id="101957at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="PTHR35805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="SSF54893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="SSF57825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PyrI" evidence="8">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="PyrI_C" evidence="9">
        <location>
            <begin position="103"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="155" mass="17404" checksum="3A23CD1E52C2BFF3" modified="2011-05-03" version="1">MKTPRELKVKPIKNGTVIDHITANKALNVLKILGLPNKETSVTIAMNVKSSQMFAKDIVKIEGRELKSGEVDKIALIAPYATINIIRNYEILDKGKVKLTNNVKGILKCPNPNCITNTPEPVKTKFQVIDKNPLILRCYYCERIVNNDDIGIQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB7</accession>
    <name>F0TCB7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645735.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10384">
        <property type="protein sequence ID" value="ADZ10384"/>
        <property type="gene ID" value="Metbo_2169"/>
    </dbReference>
    <dbReference type="GeneID" id="10278632"/>
    <dbReference type="KEGG" id="mel:Metbo_2169"/>
    <dbReference type="eggNOG" id="arCOG14016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175258_0_0_2"/>
    <dbReference type="OMA" id="YHSNKYT"/>
    <dbReference type="OrthoDB" id="114004at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="12421" checksum="5F6415C1F84A5B89" modified="2011-05-03" version="1">MAKNTDNKNEWKEIKDAEDVVWKPVRMGDEISGKYIRMEEDVGMYHSNKYTLETEDGEVDVFGSTVLDNKFKDVPLGYEVKIIYQGEKPSAPPKKPFKSFQVFLREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC3</accession>
    <name>F0TCC3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645741.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2175"/>
    <dbReference type="EnsemblBacteria" id="ADZ10390">
        <property type="protein sequence ID" value="ADZ10390"/>
        <property type="gene ID" value="Metbo_2175"/>
    </dbReference>
    <dbReference type="GeneID" id="10278638"/>
    <dbReference type="KEGG" id="mel:Metbo_2175"/>
    <dbReference type="HOGENOM" id="CLU_1821033_0_0_2"/>
    <dbReference type="OrthoDB" id="136248at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="16441" checksum="BD5EA88CE8DC5013" modified="2011-05-03" version="1">MIEEIEQRIVDNFHRDLLKSRGEKLVKCQRILPKLVEGNTEIMEKIVLSFEDTDQIDIENTYNVCIKATNMDFKACDNFNNPVDIEEIIGRRLLDYGEIYFDTFNSETSGSLRTKLIFEDDYEIKVDGNNLEIEAHIEDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG8</accession>
    <name>F0TCG8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-(Apurinic or apyrimidinic site) lyase pyrimidine dimer DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644596.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0998"/>
    <dbReference type="EnsemblBacteria" id="ADZ09245">
        <property type="protein sequence ID" value="ADZ09245"/>
        <property type="gene ID" value="Metbo_0998"/>
    </dbReference>
    <dbReference type="GeneID" id="10277447"/>
    <dbReference type="KEGG" id="mel:Metbo_0998"/>
    <dbReference type="eggNOG" id="arCOG05276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120482_0_0_2"/>
    <dbReference type="OMA" id="MRLWSIH"/>
    <dbReference type="OrthoDB" id="103700at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004260">
        <property type="entry name" value="Pyr-dimer_DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03013">
        <property type="entry name" value="Pyr_excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="17194" checksum="5DFB8633E5AEBF1E" modified="2011-05-03" version="1">MRLWSLHPKYMDSKGLVALWREGLLARKVLLGETKGYKNHPQLIRFKNHKYPLKAIDTYLFNVYLESKLRNYNFNRSKIGNKFTEELVEVTETQLVYELDHLRIKLETRDHDKYLELNDIIVPDPNPFFRKVDGDIETWEIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH3</accession>
    <name>F0TCH3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxiredoxin</fullName>
            <ecNumber evidence="5">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Thioredoxin peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644601.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1003"/>
    <dbReference type="EnsemblBacteria" id="ADZ09250">
        <property type="protein sequence ID" value="ADZ09250"/>
        <property type="gene ID" value="Metbo_1003"/>
    </dbReference>
    <dbReference type="GeneID" id="10277452"/>
    <dbReference type="KEGG" id="mel:Metbo_1003"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="OMA" id="FWYPKDF"/>
    <dbReference type="OrthoDB" id="98508at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="19"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="5 6">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-221); in linked form" evidence="5">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="5">
        <location>
            <begin position="215"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with Cys-61); in linked form" evidence="5">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="26499" checksum="0E294ACC21810D5C" modified="2011-05-03" version="1">MGEKIYVTKKIKHKEKGMPLIGDKFPKMEVQTTKGTVKLPKAFKGKWFVLFSHPADFTPVCTTEFFAFQKRYEQFKELNCELIGLSIDQIFAHLKWIEWIKDNLDVEIEFPVIADTGCVANKLGLIHPAKATNTVRAVFIIDPKGIIRAILYYPQELGRNIDEILRMIEGFNQIEEKKVAIPANWPKNELIGKGLIIPPVPDIKSGKKLADKYKCYDWWLCYRNYYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK8</accession>
    <name>F0TCK8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644636.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09285">
        <property type="protein sequence ID" value="ADZ09285"/>
        <property type="gene ID" value="Metbo_1038"/>
    </dbReference>
    <dbReference type="GeneID" id="10277487"/>
    <dbReference type="KEGG" id="mel:Metbo_1038"/>
    <dbReference type="eggNOG" id="arCOG14001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191061_0_0_2"/>
    <dbReference type="OMA" id="CYPDPCH"/>
    <dbReference type="OrthoDB" id="113564at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025475">
        <property type="entry name" value="DUF4326"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14216">
        <property type="entry name" value="DUF4326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="103" mass="11928" checksum="6522444F4EEA8E81" modified="2011-05-03" version="1">MTIRVRNKKTWENEGGEFVEYVGKGSVLENPYTHGCRRKLKDQYKSYLTKLNEDSPEWKRINELKKLHETGVDLNLLCSCYPDPCHSNVISDAITGKIKPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP6</accession>
    <name>F0TCP6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="5">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pan</name>
        <name evidence="8" type="ordered locus">Metbo_2222</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645787.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="gene ID" value="Metbo_2222"/>
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    <dbReference type="KEGG" id="mel:Metbo_2222"/>
    <dbReference type="eggNOG" id="arCOG01306">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OMA" id="YNMTTFE"/>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005937">
        <property type="entry name" value="26S_Psome_P45-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01242">
        <property type="entry name" value="26Sp45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="7">
        <location>
            <begin position="184"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="410" mass="45762" checksum="DC028028511EE190" modified="2011-05-03" version="1">MENMSPNVLKKIEDLKKEIKILKEDNTKTKRNLMWKVRKLEKDKVLIENEKMRLDREVKSLRGEIERFRSPPLVIATVTEVLDDGRVVVKSSTGPNFVIGYSRFLDEKSLEPGVRVALNQQTFSIVHVLPSEKDPLVTGMEVDEKPDVEYAQIGGLEEQVVEIKETVELPLKKPELFTNIGIEPPKGVLLYGPPGTGKTLLAKAVAHETNATFIKIVASEFVKKYIGEGARLVRGVFELAKEKSPSIIFIDEIDAIAAKRLKSSTSGDREVQRTLMQLLAEMDGFEGRGDVGIVAATNRPDILDPALLRPGRFDRFIEVPIPNEDGRMEILKIHTKNMSLDEDVDVRLVASLSEGASGADLKAICTESGMFAIREERTTVTMNDFMDAIDKIVGMEHDEEMKKEAGVMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR5</accession>
    <name>F0TCR5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S cluster domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2241</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645806.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2241"/>
    <dbReference type="EnsemblBacteria" id="ADZ10455">
        <property type="protein sequence ID" value="ADZ10455"/>
        <property type="gene ID" value="Metbo_2241"/>
    </dbReference>
    <dbReference type="GeneID" id="10278707"/>
    <dbReference type="KEGG" id="mel:Metbo_2241"/>
    <dbReference type="eggNOG" id="arCOG00295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087825_0_0_2"/>
    <dbReference type="OMA" id="CMMQKVH"/>
    <dbReference type="OrthoDB" id="63018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
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        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="25867" checksum="735A6AC9ABD2111D" modified="2011-05-03" version="1">MSSVNSNQRSKVTKLLPGFNCGICGYARCEEFAHSLLKRKSDLGKCRFLFQEIFNENRNELEEFLRQENIITPEKKPVGVLDGYEADFILNPLPKEESCREVLYPFTRKLLKKDDIIRYRPLGCPIIHFARILDEDNGLITVHMIGPCHRMDPDSEFEYMDIGICMVGGFEGLVEGPLPSVGETVRFLPGHCMMQKVHSGVVVQVVGKRVIIEGIDLKVWAPPVKGSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT8</accession>
    <name>F0TCT8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase</fullName>
            <ecNumber evidence="2">6.3.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645829.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2264"/>
    <dbReference type="EnsemblBacteria" id="ADZ10478">
        <property type="protein sequence ID" value="ADZ10478"/>
        <property type="gene ID" value="Metbo_2264"/>
    </dbReference>
    <dbReference type="GeneID" id="10278730"/>
    <dbReference type="KEGG" id="mel:Metbo_2264"/>
    <dbReference type="eggNOG" id="arCOG02818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629483_0_0_2"/>
    <dbReference type="OMA" id="VEVCDMT"/>
    <dbReference type="OrthoDB" id="51090at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="100"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="407" mass="44587" checksum="7B1E1A7FAC72AE26" modified="2011-05-03" version="1">MKVAVLGLGIEGKKSVKALLDYGASVYASDLDQSLDLSEFKSSDLDVDLGFHDKTKVDASDCVVLSPSLWNKSAINQIVGKNKLFSDVLKGHKTIFTIGVTGTNGKTTSCHMLNEILVEAGYKVLMGGNAGGGFDGYTQLTIQANESNYDVMIVEVCDMTLDFASRIFEFDMVLVTNMGYDHMEHHQSMENYRSSVDKFLKDVDQVVLNGNDPMLKGCKSRNAQYFDSEVRDLKLFGSFNQENASGAFKVASILGVEPKLINEVLSSFEAVEGRIQTLKLNGSEIIIGKTDNPDSTSAVLSEAQPDIVIIGTPRRNEKFRYNILKEVAMADPSEVVLFPGLDDTTEFARKVLINFGYSGTIIVTEDRSNLVDLVLDYTENNGKIFIGGNGQAKIMSLQSSLLQLVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT9</accession>
    <name>F0TCT9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2265"/>
    <dbReference type="EnsemblBacteria" id="ADZ10479">
        <property type="protein sequence ID" value="ADZ10479"/>
        <property type="gene ID" value="Metbo_2265"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2265"/>
    <dbReference type="eggNOG" id="arCOG06470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701346_0_0_2"/>
    <dbReference type="OMA" id="PYTYGVK"/>
    <dbReference type="OrthoDB" id="23710at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="397" mass="41968" checksum="D5BE0C950E020765" modified="2011-05-03" version="1">MKGGIILTNYLENPILILLVLIIIVVGLGTLGKSLATNIPAQNPTEFSAVNIGDTVVSGENVVNSNNIRKVPVIYHPDYLIDDLKNQEYGDFYTALTTGSVETPINEITSGNVTNTGIAKGFSGPGMVTVNGSKLVVNAPGTFVWGFKTPYTVAVKTAHGVDIKVENKTVKSVATADISNSTVSGTNMTTNSIKSWYNSSDVGDNLTIDYSLAKFNDGRNTVTPSEITSLFGANTTKYMAGYPSGSPVMVYNGATNQTVVGSGNSVLGYYAEYDNSLREVNARVFVDGWNNTIVPPHTTAYGKTNVTFVGVYDPELKSYPSHGACPSGRALRAAVMDAGFPLPSGMSSGFYAVSVDANPSSGISIYNPTDYPVKVVLWTVGGGTGMSIYAQAIQYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU1</accession>
    <name>F0TCU1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645832.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2267"/>
    <dbReference type="EnsemblBacteria" id="ADZ10481">
        <property type="protein sequence ID" value="ADZ10481"/>
        <property type="gene ID" value="Metbo_2267"/>
    </dbReference>
    <dbReference type="GeneID" id="10278733"/>
    <dbReference type="KEGG" id="mel:Metbo_2267"/>
    <dbReference type="eggNOG" id="arCOG02559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277665_0_0_2"/>
    <dbReference type="OrthoDB" id="109792at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010620">
        <property type="entry name" value="SBBP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06739">
        <property type="entry name" value="SBBP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="727"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="832"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="933"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="1034"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1141" mass="121763" checksum="F66421EA5B34688E" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV6</accession>
    <name>F0TCV6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645847.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2282"/>
    <dbReference type="EnsemblBacteria" id="ADZ10496">
        <property type="protein sequence ID" value="ADZ10496"/>
        <property type="gene ID" value="Metbo_2282"/>
    </dbReference>
    <dbReference type="GeneID" id="10278748"/>
    <dbReference type="KEGG" id="mel:Metbo_2282"/>
    <dbReference type="eggNOG" id="arCOG02644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_1_2"/>
    <dbReference type="OMA" id="CTAEIGK"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="236" mass="27498" checksum="20F5DFB5CF917B79" modified="2011-05-03" version="1">MSITDRREREKKQRQHDIINAAEKLFFSRGYDEVSMNDIALEVELSKATLYLYFQNKKSLFFAIVLRGTKILNSIIREAVKKEDKGIDKIAAYRNAYNEFTWNYPDHINIYNYFRSGRFNMEPDGSDERKTKSKSNDNSIPYEIACETEISKLRKERFSILCQSVQTGLDDGSIRQDVDPVEVSILLSAISKSLSNIPADHLQALEKRGINSESYFRDVSDLIHHMIMNWDYEQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U9</accession>
    <name>F0T5U9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP019262</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644693.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1097"/>
    <dbReference type="EnsemblBacteria" id="ADZ09342">
        <property type="protein sequence ID" value="ADZ09342"/>
        <property type="gene ID" value="Metbo_1097"/>
    </dbReference>
    <dbReference type="GeneID" id="10277547"/>
    <dbReference type="KEGG" id="mel:Metbo_1097"/>
    <dbReference type="eggNOG" id="arCOG03213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078138_0_0_2"/>
    <dbReference type="OMA" id="SKRRRMY"/>
    <dbReference type="OrthoDB" id="66534at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016754">
        <property type="entry name" value="UCP019262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09894">
        <property type="entry name" value="DUF2121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019262">
        <property type="entry name" value="UCP019262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="309" mass="34878" checksum="BE88CACE3B0513FE" modified="2011-05-03" version="1">MSLIVTYIGSKGCIMAGDKRRIGFYGSPEARETLEDELYAGKIKTEEDLIKKAEEQGINLKISDDTTKIREMDNVLVGEVISRATHETKRKRIYAISGAYSMVELTGSNIDTVKSGGRSIIVYGNKYTQELAEKTIKKYWKPKMKFVDVERIMELVMSEVSENTPSVSKSFDLMIKYNSMDQKLAKEVIRTTVLEDVKNLEAFRNQLREENIKATKNIQMATKIVIEGNIGTVTRVNGNDVEIILDQTVEALDFNWEPVAKPAERVILTSENPQNVVMGDLAVIENENLCIKRTKESLNCGFILCRSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W5</accession>
    <name>F0T5W5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1113</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644709.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1113"/>
    <dbReference type="REBASE" id="33847">
        <property type="entry name" value="M.Msp21ORF1113P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09358">
        <property type="protein sequence ID" value="ADZ09358"/>
        <property type="gene ID" value="Metbo_1113"/>
    </dbReference>
    <dbReference type="GeneID" id="10277563"/>
    <dbReference type="KEGG" id="mel:Metbo_1113"/>
    <dbReference type="eggNOG" id="arCOG02632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018284_5_0_2"/>
    <dbReference type="OMA" id="LGAYQYI"/>
    <dbReference type="OrthoDB" id="45297at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HsdM_N" evidence="4">
        <location>
            <begin position="6"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="N6_Mtase" evidence="3">
        <location>
            <begin position="139"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="505" mass="58404" checksum="EA35F7F70BF90B0D" modified="2011-05-03" version="1">MLDPELKSKINKLWDMFWSSGISNPLQAIEQMSYLIYMKRLDDDERAKEQNAKFSEEEYESLFKDCSDCRWSKWNNMAADEMLIHVREKVFPFLRDLGDENSLYRRYMKDAVFAIPTGSLLVETTSIIDEMHIKEQNLDTKGDIYEYLLSELKTSGKNGQFRTPRHIIQMMVKIVDPKVNEIICDPACGTAGFLVNSYRHILKANTSQELIKIDDEGQEYNFKGDKLSKSEYLALKNNSLFGFDFDQTMVRISLMNLMMHGISNPQIDQINTISMRYNQNPNFDVVLANPPFKGSINKDELNDDFSINTTKTEILFLELMYNILNIGGRCAVIVPQGVLFGNSRAHKSIRKKLLEDCRLDAVISMPSGVFRPYAGVSTGILIFTKGEPTAKVGFYDMEADGYTLDDKRTFIDGKGDIPDIIEKFKNSEPELFQDRKAKHFYVPYEEIKENDYSLSISNYKEIEYDEIEYESPDVIKEKILELEKKIIKGLEELDFQMNNRNNLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T656</accession>
    <name>F0T656_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Saccharop_dh_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645914.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2350"/>
    <dbReference type="EnsemblBacteria" id="ADZ10563">
        <property type="protein sequence ID" value="ADZ10563"/>
        <property type="gene ID" value="Metbo_2350"/>
    </dbReference>
    <dbReference type="GeneID" id="10278816"/>
    <dbReference type="KEGG" id="mel:Metbo_2350"/>
    <dbReference type="eggNOG" id="arCOG04422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056125_0_0_2"/>
    <dbReference type="OMA" id="GHKHHIY"/>
    <dbReference type="OrthoDB" id="22696at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="CHP00300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00300">
        <property type="entry name" value="TIGR00300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharop_dh_N" evidence="1">
        <location>
            <begin position="3"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="407" mass="44478" checksum="D028C0023609CAF3" modified="2011-05-03" version="1">MYNREVKLSGHIIDSLILPKALDRILDMGGDFKFMEFTVGKLKGDISHAKLLVEASNETLLGEILDELSEIGAVVVELKDVELLVSEKDKTLPADFYSTTHHPTEIHYNGKWITVEDIEMDCMIVVDKTAERALCRPIGQINKGDHIVVGRDGIKVMPPERPRGKQGVFEFMSSEASSEKPIESIIKKIASEIKEIKKRGGKIAVVSGPAVVHTGSGPILASMIKEGIVDIIFAGNALATHDIESALYGTSLGTCVKTGEAVRRGHRHHIYAINEINKAGSIKEAIDKGVLKKGIMYECIKNDVPYVLAGSIRDDGPLPDVITDVIEAQEEMRKYVQDVDMVIMISTMLHSIATGNLLPSHVKSICVDINPATVTKLSDRGSAQVVGIVTDVGTFLPMLYNELKTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T657</accession>
    <name>F0T657_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Adenine deaminase</fullName>
            <shortName evidence="5">Adenase</shortName>
            <shortName evidence="5">Adenine aminase</shortName>
            <ecNumber evidence="3 5">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ade</name>
        <name evidence="8" type="ordered locus">Metbo_2351</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenine + H(+) + H2O = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="3 5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645915.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2351"/>
    <dbReference type="EnsemblBacteria" id="ADZ10564">
        <property type="protein sequence ID" value="ADZ10564"/>
        <property type="gene ID" value="Metbo_2351"/>
    </dbReference>
    <dbReference type="GeneID" id="10278817"/>
    <dbReference type="KEGG" id="mel:Metbo_2351"/>
    <dbReference type="eggNOG" id="arCOG00693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027935_0_0_2"/>
    <dbReference type="OMA" id="TDHECFT"/>
    <dbReference type="OrthoDB" id="12286at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01295">
        <property type="entry name" value="AdeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="PTHR11113:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01178">
        <property type="entry name" value="ade"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="42"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Adenine_deam_C" evidence="7">
        <location>
            <begin position="364"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="538" mass="58056" checksum="86331371FDD88CA3" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T665</accession>
    <name>F0T665_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide methionine sulfoxide reductase MsrB</fullName>
            <ecNumber evidence="2">1.8.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptide-methionine (R)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">msrB</name>
        <name evidence="4" type="ordered locus">Metbo_2359</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2359"/>
    <dbReference type="EnsemblBacteria" id="ADZ10572">
        <property type="protein sequence ID" value="ADZ10572"/>
        <property type="gene ID" value="Metbo_2359"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2359"/>
    <dbReference type="eggNOG" id="arCOG02815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_5_2"/>
    <dbReference type="OMA" id="DEQWRAE"/>
    <dbReference type="OrthoDB" id="88729at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01400">
        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="PTHR10173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="3">
        <location>
            <begin position="31"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17130" checksum="E2524390DE861AE8" modified="2011-05-03" version="1">MTSNSNNENLVPIYSVKSGNVEMVEKVVKTDKEWREILSSDSYSVARKQGTELAFTGKYHDCHEDGIYECVCCGTDLFDSKAKFDSGTGWPSFWEPVASQNVKEHEDKSLGMIRIEVLCARCEAHLGHVFNDGPKPTGLRYCMNSAALKLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T679</accession>
    <name>F0T679_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644745.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1150"/>
    <dbReference type="EnsemblBacteria" id="ADZ09394">
        <property type="protein sequence ID" value="ADZ09394"/>
        <property type="gene ID" value="Metbo_1150"/>
    </dbReference>
    <dbReference type="GeneID" id="10277600"/>
    <dbReference type="KEGG" id="mel:Metbo_1150"/>
    <dbReference type="eggNOG" id="arCOG06473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159733_0_0_2"/>
    <dbReference type="OMA" id="HIYNITL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="14285" checksum="5648595FFD4C1A46" modified="2011-05-03" version="1">MDQRGQISIEYVLLVSIVLIIVVVFGLIITDQSEQNNLASAVQLGASNATANLVFGSTSQSPVKVTNVAMTGTGQNISVVVHFSRPVTDQQSTIIASIVKSLNSSGFTNVTSTTSSVTVTTSATATGTHHIYNITLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T684</accession>
    <name>F0T684_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644750.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1155"/>
    <dbReference type="EnsemblBacteria" id="ADZ09399">
        <property type="protein sequence ID" value="ADZ09399"/>
        <property type="gene ID" value="Metbo_1155"/>
    </dbReference>
    <dbReference type="GeneID" id="10277605"/>
    <dbReference type="KEGG" id="mel:Metbo_1155"/>
    <dbReference type="eggNOG" id="arCOG03821">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149768_0_0_2"/>
    <dbReference type="OrthoDB" id="126243at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="15232" checksum="16DA2385C7E591B4" modified="2011-05-03" version="1">MINDDRGQFSAEFILISLIVLIILGGLISLVGSTMDKTQTADNGGARIMGEKIAETINTAYVSGNGYSIDLDLRTMNQELSSSGNPFSFSATITNTGTGYVVLVSNAGSSSSVSVIPKRFNGTSTMNNNNLYHVKNVNGTIQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A9</accession>
    <name>F0T6A9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nitrogenase</fullName>
            <ecNumber evidence="9">1.18.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1180</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644775.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1180"/>
    <dbReference type="EnsemblBacteria" id="ADZ09424">
        <property type="protein sequence ID" value="ADZ09424"/>
        <property type="gene ID" value="Metbo_1180"/>
    </dbReference>
    <dbReference type="GeneID" id="10277630"/>
    <dbReference type="KEGG" id="mel:Metbo_1180"/>
    <dbReference type="eggNOG" id="arCOG00590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="TEIAQCY"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="31238" checksum="0FF4F6992EC803E8" modified="2011-05-03" version="1">MSKRKQIAIYGKGGIGKSTTTSNLSAALSDLGYSVMQIGCDPKNDSTTTLRKGKPIPTVMDTVRSGSNKLDNLIFEGYNGVHCVEAGGPEPGVGCAGRGIIAAIELLDNNGIIDDIDPDIVIYDVLGDVVCGGFAIPIRQGIAEQVYTVTSSDYMAIYAVNNLFKGILKYSGSGGALLGGIIGNSISKISQKEMITDFSEKTETNVIEFVPRSSTVTKCELDGVTTIEGAPNSEQAEVYRQLARNVINNKDSYIPKPFEAQDLADWAASWINNLLKEEKIEQNGIRSGSGEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B0</accession>
    <name>F0T6B0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644776.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1181"/>
    <dbReference type="EnsemblBacteria" id="ADZ09425">
        <property type="protein sequence ID" value="ADZ09425"/>
        <property type="gene ID" value="Metbo_1181"/>
    </dbReference>
    <dbReference type="GeneID" id="10277631"/>
    <dbReference type="KEGG" id="mel:Metbo_1181"/>
    <dbReference type="eggNOG" id="arCOG00592">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_4_0_2"/>
    <dbReference type="OMA" id="VVPITHC"/>
    <dbReference type="OrthoDB" id="14587at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="1">
        <location>
            <begin position="29"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="451" mass="49232" checksum="AA7E6A6EEF3772FF" modified="2011-05-03" version="1">MADNNLQTTKQTEVTDPSIHVIEAPRYSCSLAGVYGTVLGLYDAVPILHSGAGCGVGQLFGLLYGGGQNAGGDFGGTATPCSCLVEEHVIFGGENKLRSLVDSSIDLLNAELFVVISGCVPSLIGDDVDAVVGEFKDKAPVLHINAPGFKANSYEGYELFFEGLADQLLTEKPVQKGRVNILGVVPYQHIFWKGELKAIKDLLATIGIDANIIFTEYDTLEKLNDIPAAEYNVVVSPWNGHRAAKKLEDKFGTPFISFPGVPVGPKQTSEFLREVSKALNTYSDKVENVIAKEERRTYRILEYPGDAIILVRPHSYFAVAADSSTAVSLTKFLTNEIGYLPDIIQITDNPPEEFREAIIREITENLETGYSPDIIFEVDSHKIRENFKDRPFLFLFGSSLEGPIAMSEFGALSVTVAFPSYNRMVLVDNYVGYDGGARLVEHVVTTFAGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B1</accession>
    <name>F0T6B1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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            <strain evidence="2 3">AL-21</strain>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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    </dbReference>
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    </evidence>
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    </evidence>
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    <reference evidence="4" key="1">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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            <end position="179"/>
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    </feature>
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            <dbReference type="SMART" id="SM00014"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="201" mass="22563" checksum="8BF3F2B27A6C31E5" modified="2011-05-03" version="1">MNSLLSALSYLNILSFYFINHGLDNRLFDIVMPFITDFGSVLAWFLILGLMFVFGDQKTRKIAFLGVMALLIANVVVYSLKFLVAEPRPFLTLANVDLLVHAEETYSFPSGHAASSFAAAFVIGSKYKLNLKGKSYSLLYPLMIFAAVVGFSRIYIGVHYPYDVVVGAIIGILSGYFALKFWNNNLTEKISRINIKYRLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D1</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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    <dbReference type="STRING" id="877455.Metbo_1203"/>
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    </dbReference>
    <dbReference type="GeneID" id="10277653"/>
    <dbReference type="KEGG" id="mel:Metbo_1203"/>
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16649" checksum="B092BACE747F83F7" modified="2011-05-03" version="1">MKSRKRDQLKEFMDTKLVNKLRLYTAILVIMTVIVVAEVLRSKFSPELAVVGFISGMVVGILVARMYKLSWDESNNNVTGTVDVIGGIILVLYFTYIFTRTYYLEQWVSGIPLFAFILSMTAGTMFGRIINTRHDVFKILKALKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D7</accession>
    <name>F0T6D7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644803.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1209"/>
    <dbReference type="EnsemblBacteria" id="ADZ09452">
        <property type="protein sequence ID" value="ADZ09452"/>
        <property type="gene ID" value="Metbo_1209"/>
    </dbReference>
    <dbReference type="GeneID" id="10277659"/>
    <dbReference type="KEGG" id="mel:Metbo_1209"/>
    <dbReference type="eggNOG" id="arCOG02232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="OMA" id="ANACYAC"/>
    <dbReference type="OrthoDB" id="43345at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="441" mass="47686" checksum="D75D646EB585C02E" modified="2011-05-03" version="1">MIYDVIIVGTGAGGATVAHELCNENLNLLILDKGSKIPRGNAVNKIKITNLNLEIEEETDNTEYEFLSQSAELMEIVGVGGTTPVSLANACYACTSCYKNSATAQFKGHDFELFEELVDASKELKVGSLPSHMMGPATTRLMREGEKLGYFMEPMPKFIDFSKCDSCGLCILGCTKGAKWDATDFIRDVKSSENPPEIETDFTVTKILHNKGKVQGVEGIDKNGDLKVYQSKKVVLGAGALNTPQILIKSDISQDVGEGLFTDLFITVGGFLKDIELNKEIPMGVKSEFGPYFLSPHYAGQLVGMIKDKGFDVEESDVMGIMIKIADESNGKIHDDGTITKKLTEKDLKLFKKGYKKCVELLTAMGVDPDSISSTAIRGAHPGGTAAIGKVVNKNLETKIAGLYITDASVIPQAPGRPPILTITALAKRLSNNIKNEFNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F1</accession>
    <name>F0T6F1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_2372</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645936.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2372"/>
    <dbReference type="EnsemblBacteria" id="ADZ10585">
        <property type="protein sequence ID" value="ADZ10585"/>
        <property type="gene ID" value="Metbo_2372"/>
    </dbReference>
    <dbReference type="GeneID" id="10278838"/>
    <dbReference type="KEGG" id="mel:Metbo_2372"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="OMA" id="EIRCAKV"/>
    <dbReference type="OrthoDB" id="44076at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="52"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="256" mass="28914" checksum="F3A713430563FA74" modified="2011-05-03" version="1">MCSKVSKADNDLRKLLSEKRLKGDEDRKVGSEVFDRLTLETLYKLAKMGYVNQLQGAISTGKEANVFKGVDDQGNFVAVKIYRVSTSDFKKMQQYIQGDPRFNVKTSNKRQIINTWVTKEMRNLKRALEVGVKVPKPLVAKNNVLVMEFIGDDIGNPAQLMRQTIISNPDYVADKILNYVKILYNNAKLVHGDLSGYNILIDDGEPVIIDISQGVTVDNPISRELLNRDITNLVNDFKKMGVEISKDEIKSKIMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F7</accession>
    <name>F0T6F7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10591">
        <property type="protein sequence ID" value="ADZ10591"/>
        <property type="gene ID" value="Metbo_2378"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2378"/>
    <dbReference type="HOGENOM" id="CLU_3147977_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="SSF57903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5666" checksum="5ADACE73FC174362" modified="2011-05-03" version="1">MAETETVKCGRCDEWFELEREKIAKIKKEGKVKEYICPDCDEEIKANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I9</accession>
    <name>F0T6I9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645974.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2410"/>
    <dbReference type="EnsemblBacteria" id="ADZ10623">
        <property type="protein sequence ID" value="ADZ10623"/>
        <property type="gene ID" value="Metbo_2410"/>
    </dbReference>
    <dbReference type="GeneID" id="10278882"/>
    <dbReference type="KEGG" id="mel:Metbo_2410"/>
    <dbReference type="eggNOG" id="arCOG02447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="NSPQYCK"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="70" mass="7691" checksum="597664242A52F581" modified="2011-05-03" version="1">MIMVEIALDHAKCKGKECGICAYICPTNVFAIKDSEVLVNSPQYCKLCYECLETCPTAALKIKKTEGNCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J9</accession>
    <name>F0T6J9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645984.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2420"/>
    <dbReference type="EnsemblBacteria" id="ADZ10633">
        <property type="protein sequence ID" value="ADZ10633"/>
        <property type="gene ID" value="Metbo_2420"/>
    </dbReference>
    <dbReference type="GeneID" id="10278892"/>
    <dbReference type="KEGG" id="mel:Metbo_2420"/>
    <dbReference type="eggNOG" id="arCOG02465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065705_0_0_2"/>
    <dbReference type="OMA" id="EVIWGPG"/>
    <dbReference type="OrthoDB" id="73714at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PTHR43064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="1">
        <location>
            <begin position="119"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="27437" checksum="469089885583116D" modified="2011-05-03" version="1">MKDILQKLKNGEISVESAEKMLRNDHIVELEDFAKMDIHRELRTGIPEAVFAEGKDDAELLKIIKSSSNNGIIVTRLTNKRYQQMEEGINQVLTPNMAIEYNQKARILVIRTQEVKKIGKIGLITAGTSDIPVAEETRIVAEESGCEVLKSYDVGVAGIHRLFSQIRLMLDADVEVIVVVAGMEGALPSVVAGLVDVPVVGVPTSVGYGVGEGGFTALYAMLQSCAPGIAVVNIDNGFGAAIFASKIIKSRKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L3</accession>
    <name>F0T6L3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IMP cyclohydrolase</fullName>
            <ecNumber evidence="3">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purO</name>
        <name evidence="5" type="ordered locus">Metbo_2434</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2434"/>
    <dbReference type="EnsemblBacteria" id="ADZ10647">
        <property type="protein sequence ID" value="ADZ10647"/>
        <property type="gene ID" value="Metbo_2434"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2434"/>
    <dbReference type="eggNOG" id="arCOG04727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352116_0_0_2"/>
    <dbReference type="OMA" id="HVDPIAE"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="SSF75569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP_cyclohyd" evidence="4">
        <location>
            <begin position="26"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="24874" checksum="197A028BD6426387" modified="2011-05-03" version="1">MFHNPNNKFYGLFIIIIKSKKGDDVYLGRILALGTTETGNFVAYRVSSRSFPNRTAKTFEDRVSIVPTEGNEKDVFKNPYIAYNSIRIIEDKAVVSNGGHTDVIADKIASGMSIRDSMALSLLSMDYEKDDFNTPRIAGVTTLSGDSYIGIVTHEDLIVEKLEKGTCAYISTYEQIKPKTVKYDANNAQEAAQYIMDQGKFSEFTNPVTSTAAFGTDKWNLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M9</accession>
    <name>F0T6M9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heavy metal translocating P-type ATPase</fullName>
            <ecNumber evidence="10">3.6.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0009</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643613.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0009"/>
    <dbReference type="EnsemblBacteria" id="ADZ08262">
        <property type="protein sequence ID" value="ADZ08262"/>
        <property type="gene ID" value="Metbo_0009"/>
    </dbReference>
    <dbReference type="GeneID" id="10276431"/>
    <dbReference type="KEGG" id="mel:Metbo_0009"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02763">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OMA" id="HWMLPAW"/>
    <dbReference type="OrthoDB" id="21472at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016887">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
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        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIGR00003"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="773"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="76"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="811" mass="87148" checksum="4E3043E07AA47FF1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R7</accession>
    <name>F0T6R7_METLA</name>
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        <submittedName>
            <fullName evidence="5">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0047</name>
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    <organism evidence="5 6">
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    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0047"/>
    <dbReference type="EnsemblBacteria" id="ADZ08300">
        <property type="protein sequence ID" value="ADZ08300"/>
        <property type="gene ID" value="Metbo_0047"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0047"/>
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    </dbReference>
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    <dbReference type="OMA" id="FHCHTYG"/>
    <dbReference type="OrthoDB" id="18243at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CbiA_synth"/>
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    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="GATase_3"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="4">
        <location>
            <begin position="34"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CbiA" evidence="3">
        <location>
            <begin position="256"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="497" mass="54204" checksum="F6D85581B5D40293" modified="2011-05-03" version="1">MKKIGFLYVKGALPAFEDFGHLPTHIVGSNGTVNGFKMHKELDGLIIPGGSIVESQSVGRDIEREIHKMNAEGKFIFGMCSGFQLLANQTDIGRKSPCPVEKKGMGILDVSFSPMVGTDRVKAKIVDDSFLTKNLTGTLVSGFHCHTYGNITGDAKTVLKSLVKRTDYQNNPREITSGVTNDEGNAVGIMLHGALDENPMLRTNMLDFIGADDKDQTEIKDANEVLLKQIKAEMGIETGIKVINSKQEPAHGTKMIMMASTGSDSGKTFLTTGIIGALRKRGMRVAAIKVGPDIRDIVPSLYLNKEKMENFSSIKIGDLGWMDLDHVIKAVNSNNYDLVIVEGVMSIFTGLLNEKVPFSSAEIALAANLPVIMVSPCNKGGIETAAIDLTAHAEMMEKLGINTVGLILNKVYDSKIAGEAEKHIKSRINPEYFATIPKVKLKERGNMPEVEIKLEDFCLNAIKTVENYLDLDEIVKLAATPNFSGYTDFNALLKSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R9</accession>
    <name>F0T6R9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gatA</name>
        <name evidence="9" type="ordered locus">Metbo_0049</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="protein sequence ID" value="ADZ08302"/>
        <property type="gene ID" value="Metbo_0049"/>
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    <dbReference type="GeneID" id="10276472"/>
    <dbReference type="KEGG" id="mel:Metbo_0049"/>
    <dbReference type="eggNOG" id="arCOG01717">
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="459" mass="50037" checksum="9B2D7846EBB62E51" modified="2011-05-03" version="1">MKLLDKSELIKDHEITALDNLEMFCRTIGHKNDDYNAFLEINEGSAHERAEAIDKKIENGEKVGKLAGLVIGIKSNINVEDFHITAASKTLENYLGSYDATVVKRIKQEDGIIIGMTNMDEFAAGSSTETSYFGHTENPAAPGRIPGGSSGGSAVAVAAEMCDLSIGSDTGGSIRNPASHCGVVGFKPTYGVVSRQGLLDLAMSFDQIGPFSRDVSGAALLMDVIGGYDETECTSVKWDSPKFTDQLGQNSLEGTSLGVVKQFFDVSDDKIVNIVEESIDKMKDMGAEVHELSFDYIDLCLPTYYLINYVEFFSATRKYDGRKYGERIEEVCGEEVLRRIYMGSYISQKEFSGKYYKKALQARSLIKREVNKLLQNVDVIVGPTVPMLPHKIGTSLEPMDMYSYDVLTVIANLAGIPAGSMKAGEVNGIPVGIQIQGKPFEDSKILGIMSALDDADPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T7</accession>
    <name>F0T6T7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643671.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0067"/>
    <dbReference type="EnsemblBacteria" id="ADZ08320">
        <property type="protein sequence ID" value="ADZ08320"/>
        <property type="gene ID" value="Metbo_0067"/>
    </dbReference>
    <dbReference type="GeneID" id="10276490"/>
    <dbReference type="KEGG" id="mel:Metbo_0067"/>
    <dbReference type="eggNOG" id="arCOG02177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086260_0_0_2"/>
    <dbReference type="OMA" id="GYWEQHP"/>
    <dbReference type="OrthoDB" id="32299at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DUF63"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="265" mass="29355" checksum="B7AE4000A343A675" modified="2011-05-03" version="1">MIPQIMQYIRENFIYLHPGYTTLNTVVFGLILGISILLIIKMFKYIEKDPADLIIPLTPFIFFGSGARALVDNNIYPLTYLLVTPGIYLLTGFIAIITVLSAYFIEKKTQIDYRYIIFGVGAVICIPNIIFLGPINVVAALQVLGIWALISSIFVLLRNKWSLLSNKINLTVLMAHLFDASSTYVAVDFYGYSEQHVLPSALTGLVGTAAVMFPLKIAVILAALYVIDSYIEDKTIANMLKLAIFILGFAPGLRNFLSLIMGTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W7</accession>
    <name>F0T6W7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1245</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644838.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1245"/>
    <dbReference type="EnsemblBacteria" id="ADZ09487">
        <property type="protein sequence ID" value="ADZ09487"/>
        <property type="gene ID" value="Metbo_1245"/>
    </dbReference>
    <dbReference type="GeneID" id="10277696"/>
    <dbReference type="KEGG" id="mel:Metbo_1245"/>
    <dbReference type="eggNOG" id="arCOG01475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_2"/>
    <dbReference type="OMA" id="NYGHEKA"/>
    <dbReference type="OrthoDB" id="110591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="213"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="7">
        <location>
            <begin position="309"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="44809" checksum="F46B93ACC6EDAF39" modified="2011-05-03" version="1">MSNNKQIDLKKGEKAAKYSTYANLALALMKLVVGLLSGSIALLADAIHSFSDIFASVAVYIGLKLSQKGPDEKFQYGYYKFETLASLIISIIIIVTGFEIIIESVNGIISPQQMTIPSLVIAVSLISILVSYLLSRYKEKVGTEINSPALINDGKHSFVDVFSSLIVFLGILFSYLGYPIFQGFAGFVVALLIIYIGFKYGKQAVLILLDANLDPKTVQTIKSIALSQEGILGVHDIKIRSSGPYVLAELHIETEKRLTIEKADLISKKLEKLIRKELKDLDSLLIKVDPCEKTTVKIAVPIDQVNGLNSRISKHFGKAPYYLIADVDKDNIRSYALKGNPADTNEQKKGIKTAQFLINENVDILLFNGNINEGPSYALQAEMISVIKPNVENLGDSLLNAAHNDLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X4</accession>
    <name>F0T6X4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644845.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1252"/>
    <dbReference type="EnsemblBacteria" id="ADZ09494">
        <property type="protein sequence ID" value="ADZ09494"/>
        <property type="gene ID" value="Metbo_1252"/>
    </dbReference>
    <dbReference type="GeneID" id="10277703"/>
    <dbReference type="KEGG" id="mel:Metbo_1252"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065301_0_0_2"/>
    <dbReference type="OMA" id="WSPARFE"/>
    <dbReference type="OrthoDB" id="26322at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004453">
        <property type="entry name" value="QueG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30002">
        <property type="entry name" value="PTHR30002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="352" mass="39470" checksum="F8C3A3408CA9E558" modified="2011-05-03" version="1">MQEKQDNWNEIVMDMAKTLGASHVGITTKNSLGDSHQTTDLDYILDGAESAVTFAVPFGDSDLDNDIDMYLKKQDHKKLETAKVRATTLANGIALEISGLLNQVGYETVPVHANFVYRKDSPTEYRVPPLSHKLLAFRGGIGHIGYSGMIITKEYGANIALASVVTNAKLKATEALPEDDNYCNNCKLCSKVCLGDYMINEEYEDKIDGIKYKTAKKAHPMRCSYVCTGSTGYKNGNWSTWSPARFEIPESDDELIEIFQKKAIPAQNERNKINGIEGGFFHPFYPGYKIEYTCSMCQIICHPKKEVRNDRYNKLVKSGVIIEENGEKKSVTPKKADQIFDSISIDKKKLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y8</accession>
    <name>F0T6Y8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="6">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thiI</name>
        <name evidence="9" type="ordered locus">Metbo_1266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThiI family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644859.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1266"/>
    <dbReference type="EnsemblBacteria" id="ADZ09508">
        <property type="protein sequence ID" value="ADZ09508"/>
        <property type="gene ID" value="Metbo_1266"/>
    </dbReference>
    <dbReference type="GeneID" id="10277717"/>
    <dbReference type="KEGG" id="mel:Metbo_1266"/>
    <dbReference type="eggNOG" id="arCOG00038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="OMA" id="SMPEFCG"/>
    <dbReference type="OrthoDB" id="57901at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="56"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="182"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="387" mass="43158" checksum="5150B047566A9CB4" modified="2011-05-03" version="1">MDKERPIIVRYGEIGVKSPVVRKRFEKKLISNIKVLIDCKININQGRIFLFPENFEEALKSLKKIFGIVSYSPTLKTKTDHDTIKLAVQSYIKELVEKGEFDPEKTFAVKCRRVGEHKFSSREMAGFCGAAVIETTHAPVDLSNPDFTLYIEVRDDDTYIFHKKIKGAGGLPIGTQGRMISLVSGGIDSPVATYLMMKRGVDITIVNFNNHPFTSGSNEKIVKIYHKLKEYASGSDFRLLQVDYGEFLKKCTEEAPPRMTCVLCKSGMYQIAEKIAKQEKALAIIDGSSVGQVASQTLPNILATRYSTSMPVLSPLIGLDKLEISEIAEKIGTFQYSILPDSGCEAAPKHPETNAVLEKVLEVQEEIEMDSEVEKVLSTLHKVDLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z3</accession>
    <name>F0T6Z3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1271</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644864.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1271"/>
    <dbReference type="EnsemblBacteria" id="ADZ09513">
        <property type="protein sequence ID" value="ADZ09513"/>
        <property type="gene ID" value="Metbo_1271"/>
    </dbReference>
    <dbReference type="GeneID" id="10277722"/>
    <dbReference type="KEGG" id="mel:Metbo_1271"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_2"/>
    <dbReference type="OMA" id="TFCGYFA"/>
    <dbReference type="OrthoDB" id="98664at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="19"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="34064" checksum="60D6A908F4F3760B" modified="2011-05-03" version="1">MNLDVIGFGALNLDKLYHVNSIAREDEESYIQDYTESCGGSAANTVIGISRLGNKTGFVGKVGADPEGSLLLNNLQNENVNTNMVQIADTDRSGTVNGYIDEEGQRALYVDPGVNDCIKLDDVDLEVLNSSKILHLSSFVGKKYLDSIETQKTVLKNVSNAVKVSLDPGRLYVEKGFEFMDQFLSRTDILLLNLEELKFLKTNLDTGDDVVKGCDELHEEYSIDIMVVKLGSEGAYVSSNSKSEFINAFEVKCVDTTGAGDAFNAGFLHSQLKGEDVLKSGLVGNFVASNCVTEYGATRGLPDISKVEDILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T719</accession>
    <name>F0T719_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644890.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09539">
        <property type="protein sequence ID" value="ADZ09539"/>
        <property type="gene ID" value="Metbo_1297"/>
    </dbReference>
    <dbReference type="GeneID" id="10277748"/>
    <dbReference type="KEGG" id="mel:Metbo_1297"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_6_2_2"/>
    <dbReference type="OMA" id="NIMGIQI"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="333"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="489" mass="55325" checksum="A98D86A5A8606E63" modified="2011-05-03" version="1">MSSVQRLAKNTTLLFVSQMISYILGFFITMYSARYLGTAGFGVLSTALSLAYIFAVFTDLGLSTIITREVSRDKSLQTKYFTNTTILRLGLALLTFVLVVLFVNIVNLVHHQYPPQTVLVIYIIVASIIFNGLSGTFTAIFQALQKMEYQSISLILNSVLMLIGTIVVIYFNKDILFFALLYVIANVACLIFILSVYFWKYSLPKWDFDLNFIKITLIAALPLSIVSIFTLIAFRVDTVLLSLLKSSVDVGIYSAPYRLFEAFLFLPAAFTISVFPIFSQFFISSNDSLKFTYQKSFKYLTILSLPIAVGVTLLAEPIILLIYKSSFLQSVIVLQILIWTVPITFLNYIFGSILPAMNRQTTLLKITFISMIFNIVLNLWAIPHYSYIGASIVTVLTEIIVIILCFFVLSKSFCSVNLKNVIFKPAVASAVMACFILTVNINLFLEIIISIIIYFAVILLIQTFDREDHDILAQIIPIDRFKFLDRFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T747</accession>
    <name>F0T747_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646032.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2468"/>
    <dbReference type="EnsemblBacteria" id="ADZ10681">
        <property type="protein sequence ID" value="ADZ10681"/>
        <property type="gene ID" value="Metbo_2468"/>
    </dbReference>
    <dbReference type="GeneID" id="10278940"/>
    <dbReference type="KEGG" id="mel:Metbo_2468"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_0_2"/>
    <dbReference type="OMA" id="ITQSCII"/>
    <dbReference type="OrthoDB" id="83765at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="72"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="13651" checksum="DE7E23D34242FC69" modified="2011-05-03" version="1">MKDNLVAEEIMIEDVHVTSRTDVLAAAKLKMMRCNVGGLPVVDEGKLVGMITHRDILLAGGEALTLKVDDLMSKDLMVADKKTPIVEITKIMADKGYQRIPVIDDHGDLVGLITQSSLIRALAGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T752</accession>
    <name>F0T752_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2473</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646037.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2473"/>
    <dbReference type="EnsemblBacteria" id="ADZ10686">
        <property type="protein sequence ID" value="ADZ10686"/>
        <property type="gene ID" value="Metbo_2473"/>
    </dbReference>
    <dbReference type="GeneID" id="40927939"/>
    <dbReference type="KEGG" id="mel:Metbo_2473"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923202_0_0_2"/>
    <dbReference type="OMA" id="ANGWAPP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="41"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="301" mass="31530" checksum="B08278EE7D0DE84A" modified="2011-05-03" version="1" precursor="true">MKFITKILLLLVFLSSFGVSYADNDTVVYPIDPATLYSGSLTVQINPTPIHVNDTIMIIVTATNTGTADWGQLKIYMPVPKGTQFVSFVVPDRNLQNYDPDTGIWNVVQMKHFDRGQQKTAILTVKVLPSAAGKKLRATASFNTLILEGYGINMAGQASNARADVQTVLIKGDGPGDGPGDGPGDGPGDGPGDGPGDGPGDGPGDGPGTTDNANNGNTNSQDPLIHSNGDSKVISELNNYTNSQPSPLTSQSGGGGGGGKKVYDVSLPSISKNDFYSYLIAVLLIIGMIVAGYYYGIKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T758</accession>
    <name>F0T758_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646043.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2479"/>
    <dbReference type="EnsemblBacteria" id="ADZ10692">
        <property type="protein sequence ID" value="ADZ10692"/>
        <property type="gene ID" value="Metbo_2479"/>
    </dbReference>
    <dbReference type="GeneID" id="10278953"/>
    <dbReference type="KEGG" id="mel:Metbo_2479"/>
    <dbReference type="eggNOG" id="arCOG00965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387588_0_0_2"/>
    <dbReference type="OMA" id="QCGRCTA"/>
    <dbReference type="OrthoDB" id="95459at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="23303" checksum="FC33DE5115E57A74" modified="2011-05-03" version="1">MGNNCKISEKTSVRRITLEGRSGSIKDLRFKKSGFKKCIQCGRCTGSCPSTVIHKDFNPRDMMRRFMFEDLNSDHVNEIIWKCGQCYSCRSRCPRNCKAGLGVVALQHKSYVDGKAPKNIVEIAKKIRNNLYSRGETITPESYNLNLKEEFGEKTAERCSDNHKKREKLGFMPEDAREIPIPEDSLDGVRYILRSTGFKETNHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T765</accession>
    <name>F0T765_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein family UPF0066</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2486</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646050.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2486"/>
    <dbReference type="EnsemblBacteria" id="ADZ10699">
        <property type="protein sequence ID" value="ADZ10699"/>
        <property type="gene ID" value="Metbo_2486"/>
    </dbReference>
    <dbReference type="GeneID" id="10278960"/>
    <dbReference type="KEGG" id="mel:Metbo_2486"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_0_2"/>
    <dbReference type="OMA" id="VCRLEIF"/>
    <dbReference type="OrthoDB" id="117039at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="6"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="18737" checksum="47FCBD3F6724468A" modified="2011-05-03" version="1">MKTIEFKTIGTIHTPFKDLEGMPIQPIGARGVKGQIELEKEYEDGLKDLDGFSHIILIYHLHLCNGHLLEVKPFLDTEKRGIFATRSPKRPNQIGISVVRLENIEGSTLHVQDVDVVDGTPLLDIKPYIPHFDKHEADEVSIGWFEDKHQEASDKRSDRRFIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T795</accession>
    <name>F0T795_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin-guanine dinucleotide biosynthesis protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646080.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2516"/>
    <dbReference type="EnsemblBacteria" id="ADZ10729">
        <property type="protein sequence ID" value="ADZ10729"/>
        <property type="gene ID" value="Metbo_2516"/>
    </dbReference>
    <dbReference type="GeneID" id="10278990"/>
    <dbReference type="KEGG" id="mel:Metbo_2516"/>
    <dbReference type="eggNOG" id="arCOG00532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_0_1_2"/>
    <dbReference type="OMA" id="HCGYNCK"/>
    <dbReference type="OrthoDB" id="62565at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="133"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="236" mass="26448" checksum="71F854E70519D874" modified="2011-05-03" version="1">MRIIAVVGTKNTGKTTLVTKIVAELVERGFSVGTVKHTHHQFDLPERDTGKHKAAGAEIVAGIGSETFISIKDSMDLEKVLLMMKFMKDLDFVVIEGLKESKYAKISTSEFKDEFTIANVDVFNMDDAKLSTTIDLVLDRSFGMVNDLNCKKCGYESCDDFVHAKLQGRADAVYCRSESDRVILKINDTMIPLNPFVRTFIDETVLGMVKSLKTDEFGAENLNKIELLIRDKHEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C6</accession>
    <name>F0T7C6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="5">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0102</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643706.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0102"/>
    <dbReference type="EnsemblBacteria" id="ADZ08355">
        <property type="protein sequence ID" value="ADZ08355"/>
        <property type="gene ID" value="Metbo_0102"/>
    </dbReference>
    <dbReference type="GeneID" id="10276527"/>
    <dbReference type="KEGG" id="mel:Metbo_0102"/>
    <dbReference type="eggNOG" id="arCOG04184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="OrthoDB" id="94889at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20955" checksum="E5CE95F0CABDFA1E" modified="2011-05-03" version="1">MKITFITGNQHKVKEARGIFDNFGIELEHVDFGYPEIQGDLVDVAKFGAKHVAVRLGKPVIVEDAGIFIKSLKWFPGTYSSYVQDTLGNEGILKLMSDNTDRYAEFRSAVGFCTPKTEPEIFLGTVSGRIDNSELGENGFAYDPLFIPEGYSESFGELSTQEKNKFSHRRKSLEKFAVWYKDYVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G9</accession>
    <name>F0T7G9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643749.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0146"/>
    <dbReference type="EnsemblBacteria" id="ADZ08398">
        <property type="protein sequence ID" value="ADZ08398"/>
        <property type="gene ID" value="Metbo_0146"/>
    </dbReference>
    <dbReference type="GeneID" id="10276572"/>
    <dbReference type="KEGG" id="mel:Metbo_0146"/>
    <dbReference type="eggNOG" id="arCOG04064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="OMA" id="PWDQLPY"/>
    <dbReference type="OrthoDB" id="94875at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PTHR13325"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="208"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="45629" checksum="859FD75CEA4A0291" modified="2011-05-03" version="1">MNALWYYAIGFLIIWILAFAFKDKLKIDISGPILMRRTKRLRNFIDSTAQRSPRFWRGVMSLGIPVAFFFMVLMVVVLLISLQSLLSSPQIGIILPGVDIPGSQIYVPFGVGIIALMTVMVVHEFGHGILARVEGVRIKSIGVLLLAVLPGAFVEPDEEDIEKSKRISKLRIYAAGSVFNLGLALVSLILFLLISVTMLGSIFMGIPGISVPGTTIKTPNIGYNISGPIYPTFHEQGMQISSVVPGSPADGILKEGMVIQSINGIPTTNTTDYVNLQPSIQKGETLTIQTDQGTEKVTTGTNPNNATKGYIGVRSQSNLVVNQDVASKYGTILPWFFYDLSNLLYWIFLLNFAVGTFNLLPLKPLDGGLMLEELLRYRLSENIVQKIINPLSYFLIIVIVVSIVYSLGRGLFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M0</accession>
    <name>F0T7M0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09588">
        <property type="protein sequence ID" value="ADZ09588"/>
        <property type="gene ID" value="Metbo_1348"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1348"/>
    <dbReference type="HOGENOM" id="CLU_3353900_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="36" mass="4285" checksum="4910A8B15F54CE52" modified="2011-05-03" version="1">MVVKNKVCNTRKKQRRLLQKYIKGKKPVKAVEKCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M9</accession>
    <name>F0T7M9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA-adding enzyme</fullName>
            <ecNumber evidence="8">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cca</name>
        <name evidence="11" type="ordered locus">Metbo_1357</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644948.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1357"/>
    <dbReference type="EnsemblBacteria" id="ADZ09597">
        <property type="protein sequence ID" value="ADZ09597"/>
        <property type="gene ID" value="Metbo_1357"/>
    </dbReference>
    <dbReference type="GeneID" id="10277808"/>
    <dbReference type="KEGG" id="mel:Metbo_1357"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="OMA" id="DVDLVPC"/>
    <dbReference type="OrthoDB" id="82755at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="PTHR39643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="SSF55003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0692">RNA repair</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="9">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_NucTransf2" evidence="10">
        <location>
            <begin position="156"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="52759" checksum="D11F406120411CBC" modified="2011-05-03" version="1">MSELDYDTILKGIKPSKQEHEEVMKLSGDLVNVINDTASELGVEVEAALVGSVSKGTWLSGNADIDILMKFPLDTSTKLLKKYGLEIGHETIKKMEGAAEERYASHPYVTGYINDYYIDFVPCYDIKSYKELKSAVDRTLLHTKYIQANLTPKQEDEVVLLKKFMESVGTYGSEFKVGGFSGYLCELMILEYGSFKETIQNAALNWKYGHTIDLMNYQTGGNFKDPLVAVDPVDPNRNVAAALTLQKMSEFVVAAVNFHQEPNESYFKQKTHEMDEEILKELFNNRKTKTVLITFKPPNIPADAVYPQIKKTEHSIVRMVESEGFEVFGSASWTDEINEAVMLIEFEVWELPVLKKHLGPKIWIREHQKRFLDKYPDNAWIEEDRWVVGVPREYTQVLTLLSDLMSGKKSGYLQFGKHLKNRIMADHQILDVKNILDSQSNTSGFIKFLYEYLHPGDKIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S5</accession>
    <name>F0T7S5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643763.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0160"/>
    <dbReference type="EnsemblBacteria" id="ADZ08412">
        <property type="protein sequence ID" value="ADZ08412"/>
        <property type="gene ID" value="Metbo_0160"/>
    </dbReference>
    <dbReference type="GeneID" id="10276586"/>
    <dbReference type="KEGG" id="mel:Metbo_0160"/>
    <dbReference type="eggNOG" id="arCOG06493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101012_1_1_2"/>
    <dbReference type="OMA" id="ADPRSCE"/>
    <dbReference type="OrthoDB" id="104311at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14182" checksum="B097484DAEF09EF2" modified="2011-05-03" version="1">MTEGKRIDFLVEELQNDDWVVREDAAELLAEIGDPRAVDPLIKTLKDDDWHVREAAALSLAVFAEPKSLDPLTELMKDEKASVRYAAAIGLSSIADPRSCEVLKKALEDESPVVQRVAKVGIEAIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z0</accession>
    <name>F0T7Z0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643828.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0225"/>
    <dbReference type="EnsemblBacteria" id="ADZ08477">
        <property type="protein sequence ID" value="ADZ08477"/>
        <property type="gene ID" value="Metbo_0225"/>
    </dbReference>
    <dbReference type="GeneID" id="10276653"/>
    <dbReference type="KEGG" id="mel:Metbo_0225"/>
    <dbReference type="eggNOG" id="arCOG06491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639987_0_0_2"/>
    <dbReference type="OMA" id="GREGYMA"/>
    <dbReference type="OrthoDB" id="102921at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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                <person name="Brauer S.L."/>
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        </source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T825</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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        <reaction evidence="1">
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        </reaction>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ADZ09651">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09651.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A7</accession>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643870.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0267"/>
    <dbReference type="EnsemblBacteria" id="ADZ08519">
        <property type="protein sequence ID" value="ADZ08519"/>
        <property type="gene ID" value="Metbo_0267"/>
    </dbReference>
    <dbReference type="GeneID" id="10276699"/>
    <dbReference type="KEGG" id="mel:Metbo_0267"/>
    <dbReference type="eggNOG" id="arCOG03077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171166_1_0_2"/>
    <dbReference type="OMA" id="ISYIYNW"/>
    <dbReference type="OrthoDB" id="117506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10335" checksum="8A104667BDD5B511" modified="2011-05-03" version="1">MSEGIRNIIAGLALLVFAATLFQSIFHFSTMIYPGISYIYNWVGPHIAPNMVTNVVFDWRGYDTLGEALILVTAVIVTLLIFGRGQVDLGGDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H8</accession>
    <name>F0T8H8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1497</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645080.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09729">
        <property type="protein sequence ID" value="ADZ09729"/>
        <property type="gene ID" value="Metbo_1497"/>
    </dbReference>
    <dbReference type="GeneID" id="10277948"/>
    <dbReference type="KEGG" id="mel:Metbo_1497"/>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="OMA" id="EVWINDS"/>
    <dbReference type="OrthoDB" id="109784at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015217">
        <property type="entry name" value="Invasin_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09134">
        <property type="entry name" value="Invasin_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Invasin_D3" evidence="4">
        <location>
            <begin position="388"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="5">
        <location>
            <begin position="560"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="665" mass="69555" checksum="6D670D781AD0214C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I6</accession>
    <name>F0T8I6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645088.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1505"/>
    <dbReference type="EnsemblBacteria" id="ADZ09737">
        <property type="protein sequence ID" value="ADZ09737"/>
        <property type="gene ID" value="Metbo_1505"/>
    </dbReference>
    <dbReference type="GeneID" id="10277956"/>
    <dbReference type="KEGG" id="mel:Metbo_1505"/>
    <dbReference type="HOGENOM" id="CLU_2461789_0_0_2"/>
    <dbReference type="OrthoDB" id="138950at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="88" mass="10100" checksum="AE9872105AFDD658" modified="2011-05-03" version="1">MGITLWDLVGKVESIENPTVYVENYTNEEDPSKEGEIHRVEINVEDTDTEIMTGPYILEADSKQALDQQVKFTLSGYFRKVDVVNKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q1</accession>
    <name>F0T8Q1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CDP-alcohol phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643944.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0341"/>
    <dbReference type="EnsemblBacteria" id="ADZ08593">
        <property type="protein sequence ID" value="ADZ08593"/>
        <property type="gene ID" value="Metbo_0341"/>
    </dbReference>
    <dbReference type="GeneID" id="10276778"/>
    <dbReference type="KEGG" id="mel:Metbo_0341"/>
    <dbReference type="eggNOG" id="arCOG09739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599026_0_0_2"/>
    <dbReference type="OMA" id="RIARYFK"/>
    <dbReference type="OrthoDB" id="117761at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="180" mass="20352" checksum="82E9307786A410C3" modified="2011-05-03" version="1">MSPNISEHKNKLPHLITSIRILLAPIFFFSVLNLSQLDSIFIFLLTIVTDGLDGYTARKLGTTTSQGAYFDVFADFIWILAAFSAFSIVGLYPAWLIILFTFMFVQFIVTSRFKVPIYDPVGKYYGSFLFLTVFISLIFISNWINTLLTATIIIFSATSLGSRFYFLIKHEAGGNNLKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q3</accession>
    <name>F0T8Q3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3 7">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">comB</name>
        <name evidence="8" type="ordered locus">Metbo_0343</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643946.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0343"/>
    <dbReference type="EnsemblBacteria" id="ADZ08595">
        <property type="protein sequence ID" value="ADZ08595"/>
        <property type="gene ID" value="Metbo_0343"/>
    </dbReference>
    <dbReference type="GeneID" id="10276780"/>
    <dbReference type="KEGG" id="mel:Metbo_0343"/>
    <dbReference type="eggNOG" id="arCOG04871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_0_1_2"/>
    <dbReference type="OMA" id="RLFMSTT"/>
    <dbReference type="OrthoDB" id="84054at2157"/>
    <dbReference type="UniPathway" id="UPA00355">
        <property type="reaction ID" value="UER00470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00490">
        <property type="entry name" value="ComB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027639">
        <property type="entry name" value="ComB_archaeal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00298">
        <property type="entry name" value="TIGR00298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="23861" checksum="143738F5381BC547" modified="2011-05-03" version="1">MKVSLSLDSSYSEDVAVVVDVLRASTTITVAAQNFGKIIPVKSIDDAQYLAKKYDAILAGERRGAAIKGFDTGNSPREISNFHGDVLVITTSNGTRIMEGIKAKILIGSFVNARAVAKEALNLASSHIELVMAGVEGRFAIEDYLGAGEIISHLQEHNLDENAMGAYMASLNENMVNNAVKNSRSARRLGELGLSEDVNFCLKRNLYDHVPVYANGSITGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q9</accession>
    <name>F0T8Q9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0349</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643952.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0349"/>
    <dbReference type="EnsemblBacteria" id="ADZ08601">
        <property type="protein sequence ID" value="ADZ08601"/>
        <property type="gene ID" value="Metbo_0349"/>
    </dbReference>
    <dbReference type="GeneID" id="10276786"/>
    <dbReference type="KEGG" id="mel:Metbo_0349"/>
    <dbReference type="eggNOG" id="arCOG04860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="OMA" id="TAMFEMG"/>
    <dbReference type="OrthoDB" id="25508at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_beta_N" evidence="9">
        <location>
            <begin position="6"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_beta" evidence="8">
        <location>
            <begin position="187"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="441" mass="46771" checksum="985BA89A8AEDEE09" modified="2011-05-03" version="1">MAKFDDKIDLYDDRGNLVEEQVPLEALSPLRNSAIKAIVQGVKRTVAVNLEGVENALKTGKLGGGKILGRELDLDIVANAEAIAAKAAEMIQVEEGDNTNVELLVGGKRALVQIPQARFDAGAEYSAGPLVTASAFIQSIIDVCDVSMYDANMVKAAILGRYPQSVDYMGGNLATMLDVPQKLEGPGYSLRNIMVNHVVATTLKNTMNSAALSSLLEQAAMFEMGDAVGKFERMHLLGLAYQGMNADNIVFDLVKANGKEGTVGSVINDMIDRAKEDGVIGVEKDLSGFNVYGTDDMAKWNAYAAAGMMAATMVNQAAARAAQGVSSTLLYYNDILEFETGLPGVDFGRVEGTAVGFSFFSHSIYGGGGPGIFNGNHVVTRHSKGFAIPCVAAAMSLDAGTQLFSPEATSGLIKDVFSQVDAFRNPLKYVNEAAIDLKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R0</accession>
    <name>F0T8R0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-coenzyme M reductase operon protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643953.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0350"/>
    <dbReference type="EnsemblBacteria" id="ADZ08602">
        <property type="protein sequence ID" value="ADZ08602"/>
        <property type="gene ID" value="Metbo_0350"/>
    </dbReference>
    <dbReference type="GeneID" id="10276787"/>
    <dbReference type="KEGG" id="mel:Metbo_0350"/>
    <dbReference type="eggNOG" id="arCOG04859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="OMA" id="GIRRMMI"/>
    <dbReference type="OrthoDB" id="94905at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="16258" checksum="9425E759CE0484B8" modified="2011-05-03" version="1">MDIEIFPHRLLNPETTESLLNALDEIDGVKTIVLQGQRLPPEAINSDHTTITVKGEEVDLQVKTGRVLMEIDTEDTIELVRQKCEENLSFGFNVHVGTFIRKQKTVSDKIKYGEALENVPDEMVGLTDQNALLSERATILKRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R7</accession>
    <name>F0T8R7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 17">Tetrahydromethanopterin S-methyltransferase subunit B</fullName>
            <ecNumber evidence="8 17">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mtrB</name>
        <name evidence="19" type="ordered locus">Metbo_0357</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AL-21</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 17">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 17">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cell membrane</location>
            <topology evidence="3 17">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 17">Belongs to the MtrB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="8 17"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643960.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0357"/>
    <dbReference type="EnsemblBacteria" id="ADZ08609">
        <property type="protein sequence ID" value="ADZ08609"/>
        <property type="gene ID" value="Metbo_0357"/>
    </dbReference>
    <dbReference type="GeneID" id="10276794"/>
    <dbReference type="KEGG" id="mel:Metbo_0357"/>
    <dbReference type="eggNOG" id="arCOG04867">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171544_0_0_2"/>
    <dbReference type="OMA" id="TNMVYGF"/>
    <dbReference type="OrthoDB" id="114178at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01094">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008690">
        <property type="entry name" value="MtrB_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05440">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005518">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04166">
        <property type="entry name" value="methano_MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11 17 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ADZ08609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="10527" checksum="B0A0156AF5DB367D" modified="2011-05-03" version="1">MVEMLPLVKVAPELNLTLDPSTGIIGASLGREVLILSMDDINEQITELEAAADDIASALDPSTVSPGAYPGREGVYATAGKLTNMVYGFVLGLVLLVAILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T5</accession>
    <name>F0T8T5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1533"/>
    <dbReference type="EnsemblBacteria" id="ADZ09763">
        <property type="protein sequence ID" value="ADZ09763"/>
        <property type="gene ID" value="Metbo_1533"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1533"/>
    <dbReference type="eggNOG" id="arCOG11305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184181_0_0_2"/>
    <dbReference type="OMA" id="ELVGAYM"/>
    <dbReference type="OrthoDB" id="138970at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10635" checksum="B6EB466F6186ABBC" modified="2011-05-03" version="1">MLFVAEHTHPASECPMLSDAGKDMIKELFSEENINKAGIELVGAYMSCPVDESSDHKGYFIIEAPDKETIVKFFGPMKLLELREVKPFSEIAKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T941</accession>
    <name>F0T941_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644014.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0411"/>
    <dbReference type="EnsemblBacteria" id="ADZ08663">
        <property type="protein sequence ID" value="ADZ08663"/>
        <property type="gene ID" value="Metbo_0411"/>
    </dbReference>
    <dbReference type="GeneID" id="10276848"/>
    <dbReference type="KEGG" id="mel:Metbo_0411"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137753_1_0_2"/>
    <dbReference type="OMA" id="PEVHWHF"/>
    <dbReference type="OrthoDB" id="93754at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="18033" checksum="F6593749C68895D1" modified="2011-05-03" version="1">MICAYCGSIGYGRLISERSYWTIHLAPSQRYLGTCVVAIKRQCSNLSELETEEWKEFTEIVREMENALNHIFRPTLFNWSCFKNASYRDKTPHPQVHWHLIPRYDEIKFFEGIKFEDLDFGYIPQPISRKVSEDAMETMWIKISEQLNNSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T968</accession>
    <name>F0T968_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">V-type ATP synthase beta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">V-ATPase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpB</name>
        <name evidence="11" type="ordered locus">Metbo_0438</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644041.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0438"/>
    <dbReference type="EnsemblBacteria" id="ADZ08690">
        <property type="protein sequence ID" value="ADZ08690"/>
        <property type="gene ID" value="Metbo_0438"/>
    </dbReference>
    <dbReference type="GeneID" id="10276875"/>
    <dbReference type="KEGG" id="mel:Metbo_0438"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="8899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="PTHR43389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="10">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="9">
        <location>
            <begin position="136"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="463" mass="51338" checksum="DC4822BF595F4D93" modified="2011-05-03" version="1">MNTNIKTREYTTVSEVSGPLMIVEGVEGVAYSEIVDIETPAGEKRRGQVLEVKDDLAVVQVFEGTSDLNTLTTKVRFTGETAKLGVSPDMLGRIFNGTGVPIDGGPEIIPDQELDINGNPMNPSAREFPAEFIETGISTIDGMNTLVRGQKLPIFSGSGLPHNELAAQIARQAKVVGEETEFAVIFAAMGITHEEANYFMRDFERTGALERVTVFMNLADDPAIERIITPKMALTTAEYFAFELNMHVLVILTDLTNYCEALREISAARDEVPGRRGYPGYMYTDLATMYERAGRIVGKEGSITQMPILVMPQDDITHPIPDLTGYITEGQIVLSRDLFRKGIYPPVDVLPSLSRLMSGGIGEGQTREDHSGVSDQLYSAYAEGRDLRDLMAVVGEEALTERDRKFLTFADEFEKLFITQTSDEDRSIQETLDLGWQLLGLLPEAELKRVKAEHIPKYHPNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T970</accession>
    <name>F0T970_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF22 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644043.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0440"/>
    <dbReference type="EnsemblBacteria" id="ADZ08692">
        <property type="protein sequence ID" value="ADZ08692"/>
        <property type="gene ID" value="Metbo_0440"/>
    </dbReference>
    <dbReference type="GeneID" id="10276877"/>
    <dbReference type="KEGG" id="mel:Metbo_0440"/>
    <dbReference type="eggNOG" id="arCOG04850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152996_1_0_2"/>
    <dbReference type="OMA" id="LLICPYG"/>
    <dbReference type="OrthoDB" id="106591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002572">
        <property type="entry name" value="DUF22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01629">
        <property type="entry name" value="DUF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF22" evidence="1">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="13922" checksum="A999D4C3B687C9E9" modified="2011-05-03" version="1">MRIITRLDAVKKELAQAKAEKLDFQVGSISGNLRAIIANEDMEFQSGNIKSIEIKEIPIPANYLSYLSGYGSNSYGHTLAVDEEIPLPLSMERKADHAMFAAAKDCEIKKDDLLGVLILLPVHLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T974</accession>
    <name>F0T974_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold domain protein, DUF201-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ08696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="877455.Metbo_0444"/>
    <dbReference type="EnsemblBacteria" id="ADZ08696">
        <property type="protein sequence ID" value="ADZ08696"/>
        <property type="gene ID" value="Metbo_0444"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0444"/>
    <dbReference type="eggNOG" id="arCOG01595">
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    <dbReference type="OMA" id="FLCFNKQ"/>
    <dbReference type="OrthoDB" id="44157at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
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    </dbReference>
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        <property type="entry name" value="CPSASE_2"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="116"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="42586" checksum="A1976E423B8CF4E1" modified="2011-05-03" version="1">MENILVVGASTRPVAAALNSAGFNVYSADYFGCEDLLPIVKEYRSILKYESHESTGFFSEKFTHNSLNKVAAEFVDDVDHIVCCSGADPSQFPKNKILGNQEINDVENKYKLYKTLKKRFEGLINIPSTFFVDKLEDANCIVQDNPEKKFLIKPLEGSGGVGITKFTPPLETIDNNGAILQEIVDGQNISTSVISSVDEARTILVNQQLIGNMGLGQLEKYGYCGNITPFTGLDQESIHNLKEISEEIILDLGLIGSNGLDMVLKDDGTPYIIEVNPRLQGSFEVAELSLGINMAQTHINACDGSLIENVKPRCFAIKQIVFAKERSLVGNMNHEGISDLPAKNVIIEKGEPVVTVVNSGMILENTMKTSHELVASVYQNLQPAPEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A7</accession>
    <name>F0T9A7_METLA</name>
    <protein>
        <recommendedName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645209.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1632"/>
    <dbReference type="EnsemblBacteria" id="ADZ09858">
        <property type="protein sequence ID" value="ADZ09858"/>
        <property type="gene ID" value="Metbo_1632"/>
    </dbReference>
    <dbReference type="GeneID" id="10278089"/>
    <dbReference type="KEGG" id="mel:Metbo_1632"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168032_1_0_2"/>
    <dbReference type="OMA" id="RDEHDAN"/>
    <dbReference type="OrthoDB" id="113856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10980" checksum="1AC3A3E624D97F3C" modified="2011-05-03" version="1">MIYLMAIQKVEDFDKWKKVFEEHSTQRRVRGSKGAEILRDSNNPNEIVVITKWEDEDTAKKFSSSHDLKNVMKKAGVVGFPELKFLDEINQTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B1</accession>
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    </protein>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanomada group</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09862">
        <property type="protein sequence ID" value="ADZ09862"/>
        <property type="gene ID" value="Metbo_1636"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1636"/>
    <dbReference type="eggNOG" id="arCOG12085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2985693_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="57" mass="6345" checksum="F62E7FF63F08D085" modified="2011-05-03" version="1">MSNLTHKSDNGIMKVVNNGIKCQKCGYFVNKNDITKAYLKGKIDLPIQKGPATMDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C1</accession>
    <name>F0T9C1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645223.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09872">
        <property type="protein sequence ID" value="ADZ09872"/>
        <property type="gene ID" value="Metbo_1646"/>
    </dbReference>
    <dbReference type="GeneID" id="10278103"/>
    <dbReference type="KEGG" id="mel:Metbo_1646"/>
    <dbReference type="eggNOG" id="arCOG14007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811452_0_0_2"/>
    <dbReference type="OMA" id="FHWEIET"/>
    <dbReference type="OrthoDB" id="98022at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="16882" checksum="2787B82EEBAC7050" modified="2011-05-03" version="1">MEEHEQVEVKIDDDFVILVDKGLEDVVENFFHWEIETCNSCIDYKGSVWIEFCDFEEWQKFLQLALKNKIETNAVPETDTLWNFLQEKVNVKLVYDEELMEDPNNEDEVLGTGVLIICVGLTFPREYLDEFKKLFFQVFKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E8</accession>
    <name>F0T9E8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-demethylubiquinone-9 3-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1673"/>
    <dbReference type="EnsemblBacteria" id="ADZ09899">
        <property type="protein sequence ID" value="ADZ09899"/>
        <property type="gene ID" value="Metbo_1673"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1673"/>
    <dbReference type="eggNOG" id="arCOG09469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_22_1_2"/>
    <dbReference type="OMA" id="DQDEVDY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009725">
        <property type="entry name" value="3_dmu_93_MTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06983">
        <property type="entry name" value="3-dmu-9_3-mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021700">
        <property type="entry name" value="3_dmu_93_MTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="3-dmu-9_3-mt" evidence="1">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="18311" checksum="E0E09B2C5DFE1517" modified="2011-05-03" version="1">MAEIIQRITPFLWFGEDACEAAKFYVSIFKDSGIKKVVRYSAAGPGQAGSVMTVQFELEGMEFTALNGVTDIEFNQAVSLVINCESQDEIDYYWEKLTAEGEEQVCGWLKDKYGLSWQVVPKILVEYLSDSNPQRVKNVTEAMLKMVKINIEELKQAYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J1</accession>
    <name>F0T9J1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate synthase</fullName>
            <ecNumber evidence="3">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644090.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0487"/>
    <dbReference type="EnsemblBacteria" id="ADZ08739">
        <property type="protein sequence ID" value="ADZ08739"/>
        <property type="gene ID" value="Metbo_0487"/>
    </dbReference>
    <dbReference type="GeneID" id="10276927"/>
    <dbReference type="KEGG" id="mel:Metbo_0487"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="OMA" id="ATGCMEV"/>
    <dbReference type="OrthoDB" id="29720at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="42"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="31194" checksum="7A6A0CC661E279AB" modified="2011-05-03" version="1">MELPEKEFLAPGHRACAGCGATIGVRLALKMLGENTVAVSATGCLEVITTPYPETAWEIPWMHVAFENAAAVASGVESALKAQGKTDTNVVVFGGDGGTADIGLQALSGAMERGHNLIYICYDNEAYMNTGIQRSGATPYGASTTTSPHGKESFGEDKPKKNMPMIMAAHGIPYVATASISYPEDFMKKVKKAAEVDGPAYIHLQQPCTTGWGYSPAKTIDLGRLAVETGSWILYEIEDGEFKVTYRPIQRKKVSEYLNAQKRFKHLSDLEKETIQSHVDAICVELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N8</accession>
    <name>F0T9N8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645268.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1692"/>
    <dbReference type="EnsemblBacteria" id="ADZ09917">
        <property type="protein sequence ID" value="ADZ09917"/>
        <property type="gene ID" value="Metbo_1692"/>
    </dbReference>
    <dbReference type="GeneID" id="10278149"/>
    <dbReference type="KEGG" id="mel:Metbo_1692"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="GCFGCRK"/>
    <dbReference type="OrthoDB" id="60124at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="232" mass="24940" checksum="93061AC5C4C83BE6" modified="2011-05-03" version="1">MNYNKTQNETLSSKIKELIDLEISPVAVKLFIDKSEIPEGVTMVEGGEHRHCEMVQNATKGDIFYATASELQGCKGGVGALGLMDMPEPIKTGAMHHSMGKFSSRAAAKKTVDAIPKIEPTMDAIAYAPLESAPFDPDVVIIIAKPLQAMKISQGLLYNLGGRLEANFGGIQSICSDTTAGPYNTGKPNFTLGCYGSRKFAKVQADEIIVGMNGENLSELVSALEEREPEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q1</accession>
    <name>F0T9Q1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="2">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1705</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1705"/>
    <dbReference type="EnsemblBacteria" id="ADZ09930">
        <property type="protein sequence ID" value="ADZ09930"/>
        <property type="gene ID" value="Metbo_1705"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1705"/>
    <dbReference type="eggNOG" id="arCOG01076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_0_1_2"/>
    <dbReference type="OMA" id="TWAREHR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000210">
        <property type="term" value="F:NAD+ diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015376">
        <property type="entry name" value="Znr_NADH_PPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09297">
        <property type="entry name" value="zf-NADH-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="163"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="290" mass="33372" checksum="6171238FA4DC2F64" modified="2011-05-03" version="1">MNNYLSIFDEILVFMKRESIYNRYEPSVKPVSYSEEQSYWFIFKAENILINPKSSLKIPFHNDLDEISLVPLRKHYMGTLDDHPVYVVEVDAETEPWDGMEFLDLRSSYDILDEDIYLLAGRAVQIMNWDKNHRFCGKCGTETITLEDENAKICPECGFTSFTRISPAVITAIIKDDKLLMAKHSYGLKNRYALVAGFLEAGETLEEAVKREVMEEVGLEVDEIQYFGSQPWPFPNSLMVGFTAKYAGGEIKVDGKEIVDAKWFDANEVSRFPSKISIASELVEWFLDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U4</accession>
    <name>F0T9U4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645324.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1750"/>
    <dbReference type="EnsemblBacteria" id="ADZ09973">
        <property type="protein sequence ID" value="ADZ09973"/>
        <property type="gene ID" value="Metbo_1750"/>
    </dbReference>
    <dbReference type="GeneID" id="10278207"/>
    <dbReference type="KEGG" id="mel:Metbo_1750"/>
    <dbReference type="HOGENOM" id="CLU_1901946_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15359" checksum="B2F61B567F4D9CCA" modified="2011-05-03" version="1">MGGKHWTPEEDKIIISLHKQNFGPDQIYKSEKLPGRSYASILQRCIKLKIGSINKWTDKEIWMAYILYKHGLNVHEIADLLPNRSKAAVNAKLTRDALYYHKPPCEKPCDFNETKTLEKLIGNKNKKKAPKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V3</accession>
    <name>F0T9V3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Probable thymidylate kinase</fullName>
            <ecNumber evidence="2 4">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmk</name>
        <name evidence="6" type="ordered locus">Metbo_0524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="2 4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644127.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0524"/>
    <dbReference type="EnsemblBacteria" id="ADZ08776">
        <property type="protein sequence ID" value="ADZ08776"/>
        <property type="gene ID" value="Metbo_0524"/>
    </dbReference>
    <dbReference type="GeneID" id="10276969"/>
    <dbReference type="KEGG" id="mel:Metbo_0524"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="OMA" id="QMAYLFA"/>
    <dbReference type="OrthoDB" id="114844at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="5">
        <location>
            <begin position="5"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21575" checksum="7085B5A4BA72DF75" modified="2011-05-03" version="1">MYICLEGIDGSGKTTQIKLLESWIQKCGIDVLRVFEPTDNEVGKLIRKLLQNPDAINENFQITLALLFAADRTVLMRDITEAEALAKIVISDRSFYSSMVYQNDSEWIAEINKHAKQPDLVILLDINPETAVSRCDGMDSFEEQNFLASTAKKYLELADHQNFMVVNANNGINKVHDDIKRIVAHKLGMCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W3</accession>
    <name>F0T9W3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Metbo_0534</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AL-21</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644137.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0534"/>
    <dbReference type="EnsemblBacteria" id="ADZ08786">
        <property type="protein sequence ID" value="ADZ08786"/>
        <property type="gene ID" value="Metbo_0534"/>
    </dbReference>
    <dbReference type="GeneID" id="10276979"/>
    <dbReference type="KEGG" id="mel:Metbo_0534"/>
    <dbReference type="eggNOG" id="arCOG02044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018380_0_0_2"/>
    <dbReference type="OMA" id="WDNGHIY"/>
    <dbReference type="OrthoDB" id="2200at2157"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006486">
        <property type="term" value="P:protein glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="PTHR13872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="443"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ADZ08786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="816" mass="89871" checksum="0D3D3964E922F900" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z7</accession>
    <name>F0T9Z7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0569</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in the regulation of nitrogen fixation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644171.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0569"/>
    <dbReference type="EnsemblBacteria" id="ADZ08820">
        <property type="protein sequence ID" value="ADZ08820"/>
        <property type="gene ID" value="Metbo_0569"/>
    </dbReference>
    <dbReference type="GeneID" id="10277014"/>
    <dbReference type="KEGG" id="mel:Metbo_0569"/>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="OMA" id="HQIEVNF"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12495" checksum="C3A2A255BAF51F38" modified="2011-05-03" version="1">MKEITAVIRPDKLEVVKEALQEIQCNGVTVTEVKGRGRQLGITESYRGRDYKVDLLPKTRLEIIVNDDYVEKVIDAIVKTAQTGDIGDGKIFVAPVEEVIRIRTGERGEKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA08</accession>
    <name>F0TA08_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644182.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0580"/>
    <dbReference type="EnsemblBacteria" id="ADZ08831">
        <property type="protein sequence ID" value="ADZ08831"/>
        <property type="gene ID" value="Metbo_0580"/>
    </dbReference>
    <dbReference type="GeneID" id="10277025"/>
    <dbReference type="KEGG" id="mel:Metbo_0580"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101012_1_1_2"/>
    <dbReference type="OMA" id="EDYHVRE"/>
    <dbReference type="OrthoDB" id="103108at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20450" checksum="3954DC1424AAC698" modified="2011-05-03" version="1">MEREANIERLIQTLKDDDELVQTQAMGLLEEIGEPAVDKLMIAADDDDKDIRKGSIRVLGIIADPSAIDVLIESLNDPNKWVRREASTALGNMGDPAVDPLIKTLKDENWRVRGGAAWALGKIANKKAVEPLIVAMDDESGFVRGGAAWALGNMGDERGIEPLKKALNDKSSYVRRVSQKFLDKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA76</accession>
    <name>F0TA76_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Biotin-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1804</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1804"/>
    <dbReference type="EnsemblBacteria" id="ADZ10026">
        <property type="protein sequence ID" value="ADZ10026"/>
        <property type="gene ID" value="Metbo_1804"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1804"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629484_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="BPL_N" evidence="2">
        <location>
            <begin position="69"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="3">
        <location>
            <begin position="289"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="435" mass="45755" checksum="F54D36112A9BC07A" modified="2011-05-03" version="1">MGLTVSLSVLSASAANVNLNSNSSSINTLSSNNISHTVSTSKTTTSTSKTTVSTSKTTVSTTKVKTIRVLIYSGTGAINSCVSGVINGLHYSNNKNLVPGYKFSCGTTRTISSKTLSGYNVLVMPGGTSGLNYIKNVDGNAIKKFVSSGHGYVGICAGAYSGSKYVKGLYYGWGLAPHVYCNHISHEGNLLVKTSTAISSLLGPSKTLTLAHYNGPAMYTRGGNTVTFAYYADNKTGYKGQKAIVGDYYGSGRTVLSGPHPELQPTNSSLLAKMVLWAAHVNKVQQIFAVSSVNPSNGAVYVAANKQITVKYTESVKLANSNIKLQTSTGTSVPITTSVSGNTLTISHPLLSTGVKYIFTVASGTVVSSSGKTLNSYSSSFTVSPLTIAQMKDGINRVHAFQSKNNRLPNYVSFGTKQIPIATFKEIIAAYGLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB7</accession>
    <name>F0TAB7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent F420 reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644220.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0618"/>
    <dbReference type="EnsemblBacteria" id="ADZ08869">
        <property type="protein sequence ID" value="ADZ08869"/>
        <property type="gene ID" value="Metbo_0618"/>
    </dbReference>
    <dbReference type="GeneID" id="10277063"/>
    <dbReference type="KEGG" id="mel:Metbo_0618"/>
    <dbReference type="eggNOG" id="arCOG00457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_1_0_2"/>
    <dbReference type="OMA" id="IDTDVMV"/>
    <dbReference type="OrthoDB" id="83005at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="23528" checksum="30BC467DE7C8229D" modified="2011-05-03" version="1">MKIAIIGGTGDQGLGLALRFVKAGEDVIVGSRDIKKAETAVDLMKDILGSEDLPNLTAMTNQDAASNGDIIILTVPLQAQMVTLKSIKDNLKGEIFVDATSPLEGCLGGKPTRYVDLWEGSAAERSAEFLGDNAVVVSAFCNISASSLTTVDEDVECDCLVSGDDHEANLIVMELAEKISGVKSIECGALENARIVEKLTPLLINLNIRNKVKNAGIRISNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE7</accession>
    <name>F0TAE7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0648"/>
    <dbReference type="EnsemblBacteria" id="ADZ08899">
        <property type="protein sequence ID" value="ADZ08899"/>
        <property type="gene ID" value="Metbo_0648"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0648"/>
    <dbReference type="HOGENOM" id="CLU_2257388_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="103" mass="12018" checksum="5ED7BB402D041E9C" modified="2011-05-03" version="1">MITPILLIAIHIWADNNGEIKTAFSFREIFDRIGNIGWKNLIIWSIIIGSVYFVTETIRTNFDHLILGYDFVPLLVIPFLYMLMARSLTIFTAIRKIIRLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF8</accession>
    <name>F0TAF8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644261.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08910">
        <property type="protein sequence ID" value="ADZ08910"/>
        <property type="gene ID" value="Metbo_0659"/>
    </dbReference>
    <dbReference type="GeneID" id="10277104"/>
    <dbReference type="KEGG" id="mel:Metbo_0659"/>
    <dbReference type="eggNOG" id="arCOG00892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="OMA" id="WAAFTIY"/>
    <dbReference type="OrthoDB" id="44748at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="251" mass="27987" checksum="2E0566CD20BC1C95" modified="2011-05-03" version="1">MIDAHIHADTRPYEDFERMSVSGIKKAVTCAHDPLKMSTSDVVLDHIHRIMENDTKRAGENGLKLYSAVGVHPRSICNDYAIVIEKISELVEDKNVVAIGEIGLETTSKSEVEVFKEQLKLAQDLDVKVIVHTPRTHKAEVTMVTASIIEENIDTSLVLIDHVDNSIIDRVKDFDGILGLTVQPHKMTSDEAVEIMDKYGYDKFVLDSDMSSSPSDPLSVPKTVHKLRKAGVESKNIKKVSYNNAAKFFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG4</accession>
    <name>F0TAG4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644267.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0665"/>
    <dbReference type="EnsemblBacteria" id="ADZ08916">
        <property type="protein sequence ID" value="ADZ08916"/>
        <property type="gene ID" value="Metbo_0665"/>
    </dbReference>
    <dbReference type="GeneID" id="10277110"/>
    <dbReference type="KEGG" id="mel:Metbo_0665"/>
    <dbReference type="eggNOG" id="arCOG02625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065947_2_2_2"/>
    <dbReference type="OMA" id="YVVFHEM"/>
    <dbReference type="OrthoDB" id="115539at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="1">
        <location>
            <begin position="71"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="23089" checksum="254A142F471D0930" modified="2011-05-03" version="1">MIEKTLINDIEVEYEVVHRKVKNARLEIKTDKIRLVMPLNHFNTTEIIKNHEKWIYNKVVRIKQMQAESENRKLNFDLGEEEFKTMVKELILEFTKDLGVDFNRVFFKKMRSRWGSCSSKKNLNINKYLRFLPNHLIEYVVYHEVAHLVEMSHNKTFWNIIAFKYPDYKQVEDELLIYWLCIKKCLETLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG9</accession>
    <name>F0TAG9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SSS sodium solute transporter superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0670</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644272.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0670"/>
    <dbReference type="EnsemblBacteria" id="ADZ08921">
        <property type="protein sequence ID" value="ADZ08921"/>
        <property type="gene ID" value="Metbo_0670"/>
    </dbReference>
    <dbReference type="GeneID" id="10277115"/>
    <dbReference type="KEGG" id="mel:Metbo_0670"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_2_2"/>
    <dbReference type="OMA" id="AYMTMVT"/>
    <dbReference type="OrthoDB" id="8968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="534" mass="57363" checksum="C0CA6EAF9FD2945A" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN3</accession>
    <name>F0TAN3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase associated with various cellular activities AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645456.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1884"/>
    <dbReference type="EnsemblBacteria" id="ADZ10105">
        <property type="protein sequence ID" value="ADZ10105"/>
        <property type="gene ID" value="Metbo_1884"/>
    </dbReference>
    <dbReference type="GeneID" id="10278342"/>
    <dbReference type="KEGG" id="mel:Metbo_1884"/>
    <dbReference type="eggNOG" id="arCOG00441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051820_1_0_2"/>
    <dbReference type="OMA" id="YVYETRE"/>
    <dbReference type="OrthoDB" id="62125at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="36"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="33065" checksum="99E82E64BFE637C1" modified="2011-05-03" version="1">MNTHDLSMEELEEKLIENNYIPDKNTLITVYLAFKLKKPLIVEGPPGVGKTELSKAISRSFNMDFFRVQCYEGITFEQIVGEWNYQKQLLHLEMNRIKEIKETDLDVFHEEFFIKRPLLQGFINPDPSIILIDEIDKADEEVESFLLQALGESQITVNDLGTFELNNDLMVIMTSNSQRQLLDETKDRCLFLYIDYPTPEREMEIVKATLPDASQKLIESVVKRINELRNMNLVKKPSIRATIDWVKTLMNFNETELNQENFKDTVTVVLKSEDDKKKVMKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR3</accession>
    <name>F0TAR3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogenesis marker protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644290.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0688"/>
    <dbReference type="EnsemblBacteria" id="ADZ08939">
        <property type="protein sequence ID" value="ADZ08939"/>
        <property type="gene ID" value="Metbo_0688"/>
    </dbReference>
    <dbReference type="GeneID" id="10277133"/>
    <dbReference type="KEGG" id="mel:Metbo_0688"/>
    <dbReference type="eggNOG" id="arCOG02679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666680_0_0_2"/>
    <dbReference type="OMA" id="ATCFRCA"/>
    <dbReference type="OrthoDB" id="22876at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017676">
        <property type="entry name" value="Methan_mark_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03286">
        <property type="entry name" value="methan_mark_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="3">
        <location>
            <begin position="148"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="410" mass="43602" checksum="F599769829B0661C" modified="2011-05-03" version="1">MVKIAQISCGTDYSGVQKEIEKAAETFGAEIIIPEADLDYIDEAYEKFGFNAASTGIRLMIARATSIVEGKTDADAVFIATCFRCAEGALVRNEVRRFIQNNTNLPVVTYSFTERTKADELFIRMEALSTIVARKSLLAREKQEGLTLGIDSGSSTTKAVLMENNKVIGTGWLPTADVLETANIATNQALEGLDYKLDDIEGIGVTGYGRLRIGHQLNAQLIQEELSVNSKGAVYLAGKQKGEATVLDIGGMDNKVITVNDGIPDNFTMGGICAGASGRFLEMTSRRLGVDISELGPLALKGNYKNAALNSYCIVFGIQDLVTSLAAGGSKEDVAAAACYSVAEQVYEQQLQEIDLREPLIQVGGTSLIGGLVEAVSTVLGGIEVIVPEYSQYIGAVGSALLVSGLGVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU0</accession>
    <name>F0TAU0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644317.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0715"/>
    <dbReference type="EnsemblBacteria" id="ADZ08966">
        <property type="protein sequence ID" value="ADZ08966"/>
        <property type="gene ID" value="Metbo_0715"/>
    </dbReference>
    <dbReference type="GeneID" id="10277162"/>
    <dbReference type="KEGG" id="mel:Metbo_0715"/>
    <dbReference type="eggNOG" id="arCOG01355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_3_2"/>
    <dbReference type="OMA" id="PESYNWS"/>
    <dbReference type="OrthoDB" id="5945at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="202"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="515" mass="58698" checksum="60342FAAC273B0A2" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW6</accession>
    <name>F0TAW6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644343.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0741"/>
    <dbReference type="EnsemblBacteria" id="ADZ08992">
        <property type="protein sequence ID" value="ADZ08992"/>
        <property type="gene ID" value="Metbo_0741"/>
    </dbReference>
    <dbReference type="GeneID" id="10277188"/>
    <dbReference type="KEGG" id="mel:Metbo_0741"/>
    <dbReference type="eggNOG" id="arCOG05042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183480_0_0_2"/>
    <dbReference type="OMA" id="LTNYVYT"/>
    <dbReference type="OrthoDB" id="109850at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043941">
        <property type="entry name" value="EMC6-arch"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19094">
        <property type="entry name" value="EMC6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11106" checksum="92020F0ACF68B0F3" modified="2011-05-03" version="1">MEVDAKVTILHVIGGLIAAYLSYGLTTGLFNGINNEAIAIIIALIILYGFGQLAERIFGKEAVGGFKGWIWSGIVPFFFVWLVVWIILFNINPVPPINTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW8</accession>
    <name>F0TAW8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644345.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0743"/>
    <dbReference type="EnsemblBacteria" id="ADZ08994">
        <property type="protein sequence ID" value="ADZ08994"/>
        <property type="gene ID" value="Metbo_0743"/>
    </dbReference>
    <dbReference type="GeneID" id="10277190"/>
    <dbReference type="KEGG" id="mel:Metbo_0743"/>
    <dbReference type="HOGENOM" id="CLU_2127842_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="13267" checksum="071223FD39E2E27E" modified="2011-05-03" version="1">MKPKLKLNIEDGVKPGPFEGTKYLIYYTKDKFDKTDLDEVAKGLETWRMYTTSKEVEVKFSGVRQNEGKTYFALTMKFPDLFLEKHQLNDLINQVTDSFSSFLDQRIEDIIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ1</accession>
    <name>F0TAZ1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein Metbo_1917</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645488.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1917"/>
    <dbReference type="EnsemblBacteria" id="ADZ10137">
        <property type="protein sequence ID" value="ADZ10137"/>
        <property type="gene ID" value="Metbo_1917"/>
    </dbReference>
    <dbReference type="GeneID" id="10278375"/>
    <dbReference type="KEGG" id="mel:Metbo_1917"/>
    <dbReference type="eggNOG" id="arCOG03215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089549_1_0_2"/>
    <dbReference type="OMA" id="YYCPVKN"/>
    <dbReference type="OrthoDB" id="104709at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="20576" checksum="67DCBBF1EF8F833B" modified="2011-05-03" version="1">MAKSIEELDLTHKLSRNELKDVVKSEAGNIHIKDIMLAASFLRDDAQYMPKSYRDDYIERFSKSFFSRVKEIKDDKKNYPGDVNRKDLVEFLELLDEQSKNARTQQEKCFVKIARIISTYTTFVCQEPIHPVGTKFPGGFVLHIENGNYMCPVKQRQMKNPSALCKFCVSVQDKKAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB62</accession>
    <name>F0TB62_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LmbE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644364.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0762"/>
    <dbReference type="EnsemblBacteria" id="ADZ09013">
        <property type="protein sequence ID" value="ADZ09013"/>
        <property type="gene ID" value="Metbo_0762"/>
    </dbReference>
    <dbReference type="GeneID" id="10277210"/>
    <dbReference type="KEGG" id="mel:Metbo_0762"/>
    <dbReference type="eggNOG" id="arCOG03460">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025055_0_0_2"/>
    <dbReference type="OMA" id="VRYNIEM"/>
    <dbReference type="OrthoDB" id="35605at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="445" mass="50240" checksum="8527C10C86AB0C27" modified="2011-05-03" version="1" precursor="true">MDKKAKLTFFLTLILLFSFAATSYSYNNQVNNYPKYPQINSSDRVLIFAPHPDDESLSSAGLIRYCVEHNVPVHVVVVTNGGKGKLGSVRHSETLDATAKLGLSSNNVTFLDYPQVVNRLFNENWDPNHAYFDGSHHNSFSYDQNSSYCGESLESNIETMIANYNPTIIIYPAVDDDNTDHWGTGSFVDYAVNDMDYQTKMYSYLVHDDETLWPFPRSYFPQSKLLPPSYLANQTNWLIFPLNNSLEQYKFDAVNCYQSQMQKDSVFLKSFIKSNELFAVAPIKNVTRQDTSNDYINSHGFPTTVFHDPVGDILNQPNNQIYSVLDSQSSYDIGDVGFEIDNNTLWISLKTTGGISKNGIYQFHLRGFGDYGISRVDFQVQNGKASYIMPSKNSVTPTLQLRSDASGIVVGIPAMLDDHTFMIDVESENSTHVFDRTGYFNIDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB78</accession>
    <name>F0TB78_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">30S ribosomal protein S19P</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps19p</name>
        <name evidence="7" type="ordered locus">Metbo_0778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644380.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0778"/>
    <dbReference type="EnsemblBacteria" id="ADZ09029">
        <property type="protein sequence ID" value="ADZ09029"/>
        <property type="gene ID" value="Metbo_0778"/>
    </dbReference>
    <dbReference type="GeneID" id="10277226"/>
    <dbReference type="KEGG" id="mel:Metbo_0778"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_2"/>
    <dbReference type="OMA" id="VIRTHCR"/>
    <dbReference type="OrthoDB" id="99236at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_S19A/S15e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="15848" checksum="0CEC94CEB5491B8D" modified="2011-05-03" version="1">MARKVFSYRGYSLEELQDMPLDDVIQLLPSRQRRSLKRGFLPRQKKVLEKIRKIKKEGPRKDGRPQIIKTHCRDMIVLPEMVGMTFGIYSGKEFVDVTIQPEMIGCYFGEFAVTRQRVQHGDPGMGATRSSMFVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB1</accession>
    <name>F0TBB1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps9</name>
        <name evidence="5" type="ordered locus">Metbo_0811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644413.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0811"/>
    <dbReference type="EnsemblBacteria" id="ADZ09062">
        <property type="protein sequence ID" value="ADZ09062"/>
        <property type="gene ID" value="Metbo_0811"/>
    </dbReference>
    <dbReference type="GeneID" id="10277260"/>
    <dbReference type="KEGG" id="mel:Metbo_0811"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OMA" id="FMGQAEA"/>
    <dbReference type="OrthoDB" id="115425at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_S9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_S9_archaeal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="14912" checksum="6BF002650BC4D063" modified="2011-05-03" version="1">MKKVIHTSGKRKTAIARGKFRAGKGRVRINKFPVELYDPELAKLKIKEPLTLAGDLLNDVDIDVRVIGGGVMGQAEAARMVIAKGLVQWTSDMELKEKFNQYDRTMLVGDPRRSEPKKYGGPGARARKQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC5</accession>
    <name>F0TBC5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644427.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09076">
        <property type="protein sequence ID" value="ADZ09076"/>
        <property type="gene ID" value="Metbo_0825"/>
    </dbReference>
    <dbReference type="GeneID" id="10277274"/>
    <dbReference type="KEGG" id="mel:Metbo_0825"/>
    <dbReference type="eggNOG" id="arCOG04886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140211_0_0_2"/>
    <dbReference type="OMA" id="AFWDHES"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="13156" checksum="622839104B03244A" modified="2011-05-03" version="1">MKENSVISRSPLQERAKRETKSNKPKKQQVDVDETIVKKVFKKIDNKPRLAFWDHESAAVLHYLKETQPNFSMSAETAKLMRKAIKDEHPDIWRAVTTQLRDNKLDPQKNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD9</accession>
    <name>F0TBD9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645559.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10208">
        <property type="protein sequence ID" value="ADZ10208"/>
        <property type="gene ID" value="Metbo_1988"/>
    </dbReference>
    <dbReference type="GeneID" id="10278448"/>
    <dbReference type="KEGG" id="mel:Metbo_1988"/>
    <dbReference type="eggNOG" id="arCOG11304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157717_0_0_2"/>
    <dbReference type="OrthoDB" id="131979at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11583" checksum="EAE2CC670F04AE9D" modified="2011-05-03" version="1" precursor="true">MRRYEMMARLRLVLILAIVLAVVAALLYLVYGFALVWQALASGIIIAILCILIILLLGVSIFLWTKNFLIKRELKRSEMELAQCRAQLKMAVAFKEEDKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ6</accession>
    <name>F0TBJ6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Indole-3-glycerol phosphate synthase</fullName>
            <ecNumber evidence="4">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2046</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645616.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2046"/>
    <dbReference type="EnsemblBacteria" id="ADZ10265">
        <property type="protein sequence ID" value="ADZ10265"/>
        <property type="gene ID" value="Metbo_2046"/>
    </dbReference>
    <dbReference type="GeneID" id="10278508"/>
    <dbReference type="KEGG" id="mel:Metbo_2046"/>
    <dbReference type="eggNOG" id="arCOG01088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_2"/>
    <dbReference type="OMA" id="RGPHDLI"/>
    <dbReference type="OrthoDB" id="98284at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="IGPS" evidence="10">
        <location>
            <begin position="9"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="278" mass="30599" checksum="28608167AF2B89AE" modified="2011-05-03" version="1">MKTRPSISEILIKRRETLEELKEKVPLETLLDRLDSDKGNSKNNQSFKDFRGSINHDEDVAVICEFKPASPSKGHISDSKIQDALEVFKKADASAVSILTEENYFNGSLENIKIAQNIIELPILRKDFLMDEYQIYEAKLAGANAVLLMNDVYPDIKEGISLCSELELGYIVECKNREDIEHSLAADADVVGVNNRNFQNFSVDLKTTQKLSNSVPPEVVLVSESGVQGPEDAKKVAGYGADAILVGSAIMGTANTEDMHRAAKNIIKSVKGLKVGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL4</accession>
    <name>F0TBL4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0841"/>
    <dbReference type="EnsemblBacteria" id="ADZ09091">
        <property type="protein sequence ID" value="ADZ09091"/>
        <property type="gene ID" value="Metbo_0841"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0841"/>
    <dbReference type="eggNOG" id="arCOG12739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346417_0_0_2"/>
    <dbReference type="OMA" id="ENFDTHE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="22702" checksum="B325DC7A1448BF64" modified="2011-05-03" version="1" precursor="true">MILVNKSKIGIIILLIIVLGSATAYITRDSLMSYAFNQMNYNYTGYASIPNNTHNGGSLGGTYNVYGKGEHFYFKVDMKGADQYEDPLCYTSSGLIGNGTVDTINVTSNTVMDLFKKDFKSAMFETPVTGHFDMKCAAWTGYSNFTNNGTDFIGYFKINGVDTDFEGTYRFVPQGTRIAVVSSYLWWNVNSTNKTQINKTYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM3</accession>
    <name>F0TBM3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transglut_core3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644451.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0850"/>
    <dbReference type="EnsemblBacteria" id="ADZ09100">
        <property type="protein sequence ID" value="ADZ09100"/>
        <property type="gene ID" value="Metbo_0850"/>
    </dbReference>
    <dbReference type="GeneID" id="10277299"/>
    <dbReference type="KEGG" id="mel:Metbo_0850"/>
    <dbReference type="eggNOG" id="arCOG10853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129168_0_0_2"/>
    <dbReference type="OMA" id="FRPHAWL"/>
    <dbReference type="OrthoDB" id="127623at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032708">
        <property type="entry name" value="McjB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13471">
        <property type="entry name" value="Transglut_core3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Transglut_core3" evidence="2">
        <location>
            <begin position="53"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16221" checksum="D5EBE15DA19EFB70" modified="2011-05-03" version="1">MYQIKNFFELPYYKKSLLIKSLVLTIFVQTVLYIIPFSRIQTIISRITKIQIKQKKPNQIEDIIWAVLVVSSYIPRATCLVQAIAVHILLAHNGYDSTIKIGVNNLKNFEAHAWVEKKNNIILGKSEMEFVPLLNLKIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ7</accession>
    <name>F0TBQ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644485.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0885"/>
    <dbReference type="EnsemblBacteria" id="ADZ09134">
        <property type="protein sequence ID" value="ADZ09134"/>
        <property type="gene ID" value="Metbo_0885"/>
    </dbReference>
    <dbReference type="GeneID" id="10277334"/>
    <dbReference type="KEGG" id="mel:Metbo_0885"/>
    <dbReference type="eggNOG" id="arCOG02835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907971_0_0_2"/>
    <dbReference type="OrthoDB" id="77334at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007354">
        <property type="entry name" value="CruF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39419">
        <property type="entry name" value="PTHR39419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04240">
        <property type="entry name" value="Caroten_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="34547" checksum="E429A3CB3881825B" modified="2011-05-03" version="1">MEIIFFTKLLVVLIAIALILIVKPLQLKKTVYPIYFGFLIVTFLSYILTLMGVTNWLVDFVGSLWIFAGLAFVIIHSSITMGKTKTGLFFSIALIFGLVSEVLGVKYGWIYGHYYYNPMLTPAFFGLVPVINVLSWALIIYISYVFANIILDYGKNRFNPKNESSLLVFIVLILISMISAMVATNLDMLVDPVVVATHAWFWIDGGEYFGIPISNFVGWFVVVFAVSFIFRGIELFMDKKSMATEETPTHSATIISLYIMFLLIYGYTALLIGHPEYLLVGVTAMGPFILITMLITYLKFKKEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR6</accession>
    <name>F0TBR6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intimin/invasin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0894"/>
    <dbReference type="EnsemblBacteria" id="ADZ09143">
        <property type="protein sequence ID" value="ADZ09143"/>
        <property type="gene ID" value="Metbo_0894"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0894"/>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_282087_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003344">
        <property type="entry name" value="Big_1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02369">
        <property type="entry name" value="Big_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="3">
        <location>
            <begin position="343"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1108" mass="121494" checksum="D90B117F93EA4BDB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX0</accession>
    <name>F0TBX0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nitrogenase iron protein</fullName>
            <ecNumber evidence="12">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nifH</name>
        <name evidence="15" type="ordered locus">Metbo_2093</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">The reversible ADP-ribosylation of Arg-98 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 14">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645663.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2093"/>
    <dbReference type="EnsemblBacteria" id="ADZ10312">
        <property type="protein sequence ID" value="ADZ10312"/>
        <property type="gene ID" value="Metbo_2093"/>
    </dbReference>
    <dbReference type="GeneID" id="10278556"/>
    <dbReference type="KEGG" id="mel:Metbo_2093"/>
    <dbReference type="eggNOG" id="arCOG00590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="YVCDYYL"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12 13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="12 13">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ10312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="29296" checksum="D5FFBEC0DD42DB31" modified="2011-05-03" version="1">MSKTKKIAIYGKGGIGKSTTVSNIAAARSKDSEVLVIGCDPKADTTRTLVGRRIPTILDILKLKKKDLNQEDIIVKGFGDVKCVESGGPEPGVGCAGRGVIVAMNLLERLGVFKENLDLIIYDVLGDVVCGGFAVPLRENFADEVYIVTSGEYMSLYAANNICKGIKKLKGNLGGIICNCRGIENELEIVEEFAKIVNTKIIGVMPRNELVQKAEIEAKTVIEKFPDSEQAELYIELGENIYNNQKFDIPKPMEVDDFEKFFRKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX3</accession>
    <name>F0TBX3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645666.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2096"/>
    <dbReference type="EnsemblBacteria" id="ADZ10315">
        <property type="protein sequence ID" value="ADZ10315"/>
        <property type="gene ID" value="Metbo_2096"/>
    </dbReference>
    <dbReference type="GeneID" id="10278559"/>
    <dbReference type="KEGG" id="mel:Metbo_2096"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_2"/>
    <dbReference type="OMA" id="MQITMLV"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="13"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="484" mass="52088" checksum="B47A2DC57DC1D854" modified="2011-05-03" version="1">MEPSKNEFNISLVMAGLMIGLLVAALDNSIMATAMPKVISSLGGMVYYVWPFTIYMLTSTIAIILFGKLSDLYGRKRILIAGIVLFVLSSVACGFSSNMLQLIIFRGIQGIGGGILITIPFIVVAELFPPRQRGKYTGILASVFGISNVLGPILGGVITDFMGWQWVFFVNVPVGVAAIVMLSIYFPHLKQVVREKVIDYAGLITMSLSFSSLCVALTYVRSSYLPEYVVGFLFIFAALMFILFIHVERRAVEPLLPMHLFKISVFNLSAVAMFLSNAVMFCGIIYIPLFIQKVLGISASGSGALITPMLVSLTVASLIAGQVISKTGTYKKMGVMAFGLITVGMVMATTITQSTGTAEILIYTTILGIGSGIMYPVFTVAIQNAVLRRDIGIATASSQFFRNVGATITLPIFGLIVNLTMNMDINAVSSVPVGPMITAIHNVFTFGIVMCIIGLIASLMLKDAVLSNSNDFNVIDEPVGTEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY3</accession>
    <name>F0TBY3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Thioesterase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645676.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2106"/>
    <dbReference type="EnsemblBacteria" id="ADZ10325">
        <property type="protein sequence ID" value="ADZ10325"/>
        <property type="gene ID" value="Metbo_2106"/>
    </dbReference>
    <dbReference type="GeneID" id="10278569"/>
    <dbReference type="KEGG" id="mel:Metbo_2106"/>
    <dbReference type="eggNOG" id="arCOG01137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_2_1_2"/>
    <dbReference type="OMA" id="VIPVWFE"/>
    <dbReference type="OrthoDB" id="128167at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="139" mass="16265" checksum="9E19F054A0FAD116" modified="2011-05-03" version="1">MFKTVVTPRFGDIDGLRHVNNTVLAIWFEEARNPVFRMFTPDLDLSYENWKLIMVRTEFDFVGEMYYGEDIEIRTFVEKVGNSSFTLGHEAWQRGKLNVRGKAVVVHFDFIEKKSMPIPESIRSQLEEHLLPHGTQSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC22</accession>
    <name>F0TC22_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644524.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09173">
        <property type="protein sequence ID" value="ADZ09173"/>
        <property type="gene ID" value="Metbo_0924"/>
    </dbReference>
    <dbReference type="GeneID" id="24964526"/>
    <dbReference type="KEGG" id="mel:Metbo_0924"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901980_0_0_2"/>
    <dbReference type="OrthoDB" id="114649at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="26"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="30835" checksum="CBFD38867528DE9C" modified="2011-05-03" version="1" precursor="true">MSVLLLFSLALILNVNTSAAASSNATLKVSSVDPANNAIIHDSQSIKVKYNKTVKSGKVWVVLKKGTGKSVTVNTEVKGNTLTITPKQALGIGKYSLTIHSGTVKDENGIGNKLLKTSFTVSPLTLAQMKDGIKRAEKYYANNGRLPNTVSYGSTKYSITQFNQIISTQGLTIKKPKVVSAAAAVSDVMKKASKYSYSSAAHTGKDMERIGSGDCWAMSDYLYTHLTAKGVKSRIIQYGTAYSSNHRSVQYYSNNRWVDVPYRTYFTTSMFNNTQSYGTVIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC35</accession>
    <name>F0TC35_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644537.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0939"/>
    <dbReference type="EnsemblBacteria" id="ADZ09186">
        <property type="protein sequence ID" value="ADZ09186"/>
        <property type="gene ID" value="Metbo_0939"/>
    </dbReference>
    <dbReference type="GeneID" id="10277388"/>
    <dbReference type="KEGG" id="mel:Metbo_0939"/>
    <dbReference type="eggNOG" id="arCOG00429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018957_0_0_2"/>
    <dbReference type="OMA" id="IGYGPDF"/>
    <dbReference type="OrthoDB" id="1910at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="124"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="724" mass="79745" checksum="0B445824F1F37A0A" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA5</accession>
    <name>F0TCA5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage/plasmid primase, P4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645723.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2157"/>
    <dbReference type="EnsemblBacteria" id="ADZ10372">
        <property type="protein sequence ID" value="ADZ10372"/>
        <property type="gene ID" value="Metbo_2157"/>
    </dbReference>
    <dbReference type="GeneID" id="10278620"/>
    <dbReference type="KEGG" id="mel:Metbo_2157"/>
    <dbReference type="eggNOG" id="arCOG06914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_466632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006500">
        <property type="entry name" value="DNA_primase_phage/plasmid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045455">
        <property type="entry name" value="DUF5906"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014015">
        <property type="entry name" value="Helicase_SF3_DNA-vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014818">
        <property type="entry name" value="Phage/plasmid_primase_P4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08706">
        <property type="entry name" value="D5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19263">
        <property type="entry name" value="DUF5906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01613">
        <property type="entry name" value="primase_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51206">
        <property type="entry name" value="SF3_HELICASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SF3 helicase" evidence="2">
        <location>
            <begin position="303"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="584" mass="67629" checksum="3187C8F5F6E76534" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC6</accession>
    <name>F0TCC6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0440 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645744.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2178"/>
    <dbReference type="EnsemblBacteria" id="ADZ10393">
        <property type="protein sequence ID" value="ADZ10393"/>
        <property type="gene ID" value="Metbo_2178"/>
    </dbReference>
    <dbReference type="GeneID" id="10278641"/>
    <dbReference type="KEGG" id="mel:Metbo_2178"/>
    <dbReference type="eggNOG" id="arCOG05854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182712_0_0_2"/>
    <dbReference type="OMA" id="YLNPISR"/>
    <dbReference type="OrthoDB" id="130322at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024502">
        <property type="entry name" value="DUF3194"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035954">
        <property type="entry name" value="MTH677-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11419">
        <property type="entry name" value="DUF3194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110783">
        <property type="entry name" value="SSF110783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="86" mass="9735" checksum="4CEF589C9B7338AB" modified="2011-05-03" version="1">MRKLSDQELDEISELAVKSAETFIYSRVSKKEILDLDIKVELDYDKGLDVDIDIDIKFDPLSKSDNTIAEEAANHAITQIDSYLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN6</accession>
    <name>F0TCN6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644664.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09313">
        <property type="protein sequence ID" value="ADZ09313"/>
        <property type="gene ID" value="Metbo_1067"/>
    </dbReference>
    <dbReference type="GeneID" id="10277516"/>
    <dbReference type="KEGG" id="mel:Metbo_1067"/>
    <dbReference type="HOGENOM" id="CLU_2476027_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="87" mass="10631" checksum="A58557B588ABA71F" modified="2011-05-03" version="1">MKKNLTNTPMNSNQSIKENKKRIIVLEARKDRLNEEIEKLEKKTNKNLIEKTKIEHLKHELKSRYDQIDEHRRYLDRSKELLEDMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR4</accession>
    <name>F0TCR4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F420_ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645805.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2240"/>
    <dbReference type="EnsemblBacteria" id="ADZ10454">
        <property type="protein sequence ID" value="ADZ10454"/>
        <property type="gene ID" value="Metbo_2240"/>
    </dbReference>
    <dbReference type="GeneID" id="10278706"/>
    <dbReference type="KEGG" id="mel:Metbo_2240"/>
    <dbReference type="eggNOG" id="arCOG02715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965090_0_0_2"/>
    <dbReference type="OMA" id="WSKYLWG"/>
    <dbReference type="OrthoDB" id="73785at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012030">
        <property type="entry name" value="UCP006563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006563">
        <property type="entry name" value="UCP006563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_ligase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="30906" checksum="F18AA1651D6AAC6F" modified="2011-05-03" version="1">MEYRTIPVNTGYIKPNESYDIIIDNASSLLEDGDFLVISETPISVSQGRLVDESEFEPSILSILLADVWSKYLWGYLLGPIFRIKPRTIKNLRKLPNEARTHKKVILEYYGIKHALKPASEAGVDLSNVPGSMVSLLPDDPTTVSKDISSKIKLNHGKNVTVLIIDTDASYQIFNTKFTSLPIAVEGIRSDMGFFGYLLGRFSTLLGPTPLGISESIDLEQILEIAAAAENFHKSNETDIETVYDMSKAFEGDIADVTIEMLDSVKHTPAVIVRSLKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS2</accession>
    <name>F0TCS2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secE</name>
        <name evidence="10" type="ordered locus">Metbo_2248</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645813.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2248"/>
    <dbReference type="EnsemblBacteria" id="ADZ10462">
        <property type="protein sequence ID" value="ADZ10462"/>
        <property type="gene ID" value="Metbo_2248"/>
    </dbReference>
    <dbReference type="GeneID" id="10278714"/>
    <dbReference type="KEGG" id="mel:Metbo_2248"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191921_0_1_2"/>
    <dbReference type="OMA" id="DFLHQCR"/>
    <dbReference type="OrthoDB" id="131124at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="SSF103456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="61" mass="6604" checksum="9C312B81B7B17CA2" modified="2011-05-03" version="1">MNLNKKSLVDFIKLCQRVLHVSRKPGREEFLNVAKITGLGVIIIGAIGFIISIGAQLLSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT4</accession>
    <name>F0TCT4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2260"/>
    <dbReference type="EnsemblBacteria" id="ADZ10474">
        <property type="protein sequence ID" value="ADZ10474"/>
        <property type="gene ID" value="Metbo_2260"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2260"/>
    <dbReference type="HOGENOM" id="CLU_3210747_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="44" mass="5099" checksum="D153076B214C5F9D" modified="2011-05-03" version="1">MVMILRDSQAIESPKNPQNFFQKKTISKSQMAPEIKVSNLLFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU4</accession>
    <name>F0TCU4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L40e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl40e</name>
        <name evidence="5" type="ordered locus">Metbo_2270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645835.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2270"/>
    <dbReference type="EnsemblBacteria" id="ADZ10484">
        <property type="protein sequence ID" value="ADZ10484"/>
        <property type="gene ID" value="Metbo_2270"/>
    </dbReference>
    <dbReference type="GeneID" id="10278736"/>
    <dbReference type="KEGG" id="mel:Metbo_2270"/>
    <dbReference type="eggNOG" id="arCOG04049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_205640_0_0_2"/>
    <dbReference type="OrthoDB" id="127293at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_L40e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_L40e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="PTHR39649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L40e" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5564" checksum="D19663CABD62024E" modified="2011-05-03" version="1">MARFEEAENRLFKIKICLKCNARNPPTAKTCRKCGYKGLRYKAKEARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S7</accession>
    <name>F0T5S7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644671.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09320">
        <property type="protein sequence ID" value="ADZ09320"/>
        <property type="gene ID" value="Metbo_1075"/>
    </dbReference>
    <dbReference type="GeneID" id="10277524"/>
    <dbReference type="KEGG" id="mel:Metbo_1075"/>
    <dbReference type="HOGENOM" id="CLU_1313134_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="209" mass="23322" checksum="D6C103D79FA3F135" modified="2011-05-03" version="1" precursor="true">MKEYKKIGNFKLGLLFLGILFVFSLSVSPSAATHVDTIYVISHGNCNGHSLVYNDKTFSNPKATQLNSTKSIQNKGNTDDRAVKTSEKGPSKYKAPAVPGGQITSPSNEYNGYVYPGSIDVVKGEKFFVQRPFDNWIVCNKGSIFWDDQGYWNPIYDTGKGITLLEVNVFGAWFECDGIISNGTSIKWKTMDYTNPYRVNVRMKVFGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V9</accession>
    <name>F0T5V9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644703.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1107"/>
    <dbReference type="EnsemblBacteria" id="ADZ09352">
        <property type="protein sequence ID" value="ADZ09352"/>
        <property type="gene ID" value="Metbo_1107"/>
    </dbReference>
    <dbReference type="GeneID" id="10277557"/>
    <dbReference type="KEGG" id="mel:Metbo_1107"/>
    <dbReference type="eggNOG" id="arCOG03127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044435_0_0_2"/>
    <dbReference type="OMA" id="DLTCKYG"/>
    <dbReference type="OrthoDB" id="17294at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="392" mass="45858" checksum="5C6ABA1D59A02692" modified="2011-05-03" version="1">MRCKYKYEYFPCYEDTYKNYDYCILHLQPNMDFNEDDMYKIEKLKHIKINEKIKNGDFNFDGIFVRDIYFENMEISKDMSFRGALFEEFHFNSSIVTGNIDFSDAKITKWVDFTKSKIDGNAIFTSAKLPYKGLFEKFKVKYNVDFENCSLEGYLALNESNIGGRLNFASTKMESLNLNSSRIGSLILDHTQLTGNLYAQNAAIWGMVVNNSSAKFMNFDESKITKDVELYSNSFKEGISFDKTSFIVPLSQEKTSRYVKNIWEKLGDRNKADYYFYREMEAKRLQKGKLKRFLEYVFVQWAFEYGSNPYKVLGLWLILILGFSLIYGILNAVEGIANPLDYIYFSVFAGTGFDYASYHLKDGFQFLASIEVIFAIFIWGAFLVIFSRKYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X5</accession>
    <name>F0T5X5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09368">
        <property type="protein sequence ID" value="ADZ09368"/>
        <property type="gene ID" value="Metbo_1123"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1123"/>
    <dbReference type="HOGENOM" id="CLU_2340201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12258" checksum="05B0BA62E0ECB1D3" modified="2011-05-03" version="1">MKSYISIIIIIIGVIAFLVTRIVHDPSYNFSLGAIIIIIIGLIWMYFDSKGPDRKFNFRKTDSVDNCTKCKYYNKRSFNGVNADCKFLYIKVDEFHVCDLYKKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y2</accession>
    <name>F0T5Y2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644726.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09375">
        <property type="protein sequence ID" value="ADZ09375"/>
        <property type="gene ID" value="Metbo_1131"/>
    </dbReference>
    <dbReference type="GeneID" id="10277581"/>
    <dbReference type="KEGG" id="mel:Metbo_1131"/>
    <dbReference type="HOGENOM" id="CLU_1811451_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="15277" checksum="B15E5F4FFECD9BD8" modified="2011-05-03" version="1">MNDISIIFGILAIGILIVFIVLLLNDKLKLHLYNPIIATAIGVVTVFIITPSITELAFYFHLSFFVITISFGIGAFVGGFITVLLSNKSPLIGILPIIPYIITVLLTIGAVESALIISSINIIFALVGSYIGNKTKKSIINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y8</accession>
    <name>F0T5Y8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1137"/>
    <dbReference type="EnsemblBacteria" id="ADZ09381">
        <property type="protein sequence ID" value="ADZ09381"/>
        <property type="gene ID" value="Metbo_1137"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1137"/>
    <dbReference type="HOGENOM" id="CLU_3227814_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="5307" checksum="27863296537168C6" modified="2011-05-03" version="1">MIQQLTEELKSEYNKYRNKKKLPVNIRRVKRFLEIEGVLKEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z4</accession>
    <name>F0T5Z4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UvrABC system protein C</fullName>
            <shortName evidence="6">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">uvrC</name>
        <name evidence="11" type="ordered locus">Metbo_1143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1143"/>
    <dbReference type="EnsemblBacteria" id="ADZ09387">
        <property type="protein sequence ID" value="ADZ09387"/>
        <property type="gene ID" value="Metbo_1143"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1143"/>
    <dbReference type="eggNOG" id="arCOG00873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_2_2"/>
    <dbReference type="OMA" id="HIECFDN"/>
    <dbReference type="OrthoDB" id="5341at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 6" id="KW-0228">DNA excision</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 6" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="GIY-YIG" evidence="9">
        <location>
            <begin position="13"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="8">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="UVRC" evidence="10">
        <location>
            <begin position="245"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="590" mass="67884" checksum="A7C55FDF30140ACA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T612</accession>
    <name>F0T612_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645870.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10519">
        <property type="protein sequence ID" value="ADZ10519"/>
        <property type="gene ID" value="Metbo_2305"/>
    </dbReference>
    <dbReference type="GeneID" id="10278771"/>
    <dbReference type="KEGG" id="mel:Metbo_2305"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444562_0_0_2"/>
    <dbReference type="OMA" id="TANIANC"/>
    <dbReference type="OrthoDB" id="143805at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="592" mass="61377" checksum="05B15AEF59F52B4F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T625</accession>
    <name>F0T625_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PASTA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645883.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10532">
        <property type="protein sequence ID" value="ADZ10532"/>
        <property type="gene ID" value="Metbo_2318"/>
    </dbReference>
    <dbReference type="GeneID" id="10278784"/>
    <dbReference type="KEGG" id="mel:Metbo_2318"/>
    <dbReference type="HOGENOM" id="CLU_992516_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="2">
        <location>
            <begin position="89"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="31609" checksum="8BCD3D73CC7013EC" modified="2011-05-03" version="1">MSDEKELTKFLGSSVLIILISLLISYIIPQSLTNQSAIYWLICIIIICLVMVTFLLLFKWYFNTSMKAFILILSIFIVFTIAFSYYFYSNDFATTPNLISKSSGDAENLLNKTGLTYNVTYKIVNNSSYVNTVLSQNPKDNVLVHKNSMINIVVGQLESIIFINQPQNHDYVEQNITVSGKVKNLQNGDKVYLLVQPQPISGDGPYEWYIQPTPTHPISVDVQTDGTWKCNAYLGSQGDIGRQFKIVAIVTNLDYSNNKVGFDLPNYKSISNIIYVTRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T632</accession>
    <name>F0T632_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BioY protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BioY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645890.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2326"/>
    <dbReference type="EnsemblBacteria" id="ADZ10539">
        <property type="protein sequence ID" value="ADZ10539"/>
        <property type="gene ID" value="Metbo_2326"/>
    </dbReference>
    <dbReference type="GeneID" id="10278792"/>
    <dbReference type="KEGG" id="mel:Metbo_2326"/>
    <dbReference type="eggNOG" id="arCOG02986">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077931_2_0_2"/>
    <dbReference type="OMA" id="AGYLWSY"/>
    <dbReference type="OrthoDB" id="92598at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="PTHR34295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="22328" checksum="3FB1536C92BC5A97" modified="2011-05-03" version="1">MEITIDNYFEKRYTLFKWRSETSNANKLVMAFFMACLTGLMAQIIIPLPWTPVPITAQTFAVLFAGIVLGRYWGGLSMLIYIAIGLVGVPWFGGMTSGVEILFGASGGFLVGFVLAALVVGYLSDRYVKVRNFMPMFTLLVFANFLLIYVPGVIGLAVFMYIANGTVPGVWTLLAMGVLPFVIGDLVKIGGAAALAKAVTPKDSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T635</accession>
    <name>F0T635_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase associated with various cellular activities AAA_5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2329</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645893.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2329"/>
    <dbReference type="EnsemblBacteria" id="ADZ10542">
        <property type="protein sequence ID" value="ADZ10542"/>
        <property type="gene ID" value="Metbo_2329"/>
    </dbReference>
    <dbReference type="GeneID" id="10278795"/>
    <dbReference type="KEGG" id="mel:Metbo_2329"/>
    <dbReference type="eggNOG" id="arCOG02162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550564_0_0_2"/>
    <dbReference type="OMA" id="HDPYGGH"/>
    <dbReference type="OrthoDB" id="7657at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="AAA" evidence="5">
        <location>
            <begin position="45"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="503" mass="57019" checksum="9799BEAB801085B1" modified="2011-05-03" version="1">MYVNMKKEFLRDINTTKDILIPKDPLERVIGHEDIIRSVKIAAKQRRNLLLVGPPGIGKSLIAQAISFHLKKPNEEITVVQNPERPERPFVETKTRNEMEHEKRDLERAEGELVSPENVPEAVADRLGFRCVNCGGYNNAYQSICPNCGADKYSHINARRKHLGDLLGMFEMNEGSVNIPQERVTTTRMKNGREEVVIYERVDGEHIKILDQHALEKRRELVEEKPKNVIIPIDRKLFIQATGASETELLGDVRHDPYGGHPELGTQPYERVVPGAVHEAHEGVLFIDEIIHIAPLQRYILSAMQDKVFPIVGRNPQSAGSSVRVDNVPCDFIFIAACNIRDIQHILPPLRSRIQGEGYEILMNTTMPDNDENRAKLAQFVAQEIKLDGKIPHATRRAVEVLIEEASRRAESIDEQKNSLTLRLRDLGGCLRMAGDMAVMESSDLIMENHMSFAIKNALSIEDQIIKRYRTFENAFNKDISSSQNMGTGQKQNPNENVDRSYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T650</accession>
    <name>F0T650_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ordered locus">Metbo_2344</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645908.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2344"/>
    <dbReference type="EnsemblBacteria" id="ADZ10557">
        <property type="protein sequence ID" value="ADZ10557"/>
        <property type="gene ID" value="Metbo_2344"/>
    </dbReference>
    <dbReference type="GeneID" id="10278810"/>
    <dbReference type="KEGG" id="mel:Metbo_2344"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_2_2"/>
    <dbReference type="OMA" id="VNLGHVG"/>
    <dbReference type="OrthoDB" id="104144at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="135"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="176"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30704" checksum="D5B9CD617E87E253" modified="2011-05-03" version="1">MRIGVVSRFDVEEAVEMAEKIVEFLMENEVETLIDSQLATHLKQYEGIASDLEDMDVDLVVAVGGDGTILRTQGIINHKKIPLVGINKGAVGFLTEIDPEDAVTALEQVLNGDYFVEKRTQLKICHNKQLHSALNEVVLMTQKPAKMLHIEISVDDEVVEELRADGLIVATPSGSTAYSMSAGGPIVDPRVDAFIIVPICPFKLGARPFVVPNDSVIKVKFLRKGKKAVAVIDGQQVEEIDYMDEIIFRKADNYAYFVRLSKNFYKRVREKLQGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T652</accession>
    <name>F0T652_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="6">PvlArgDC</shortName>
            <ecNumber evidence="6">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdaD</name>
        <name evidence="7" type="ordered locus">Metbo_2346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H(+) + L-arginine = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="6">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645910.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2346"/>
    <dbReference type="EnsemblBacteria" id="ADZ10559">
        <property type="protein sequence ID" value="ADZ10559"/>
        <property type="gene ID" value="Metbo_2346"/>
    </dbReference>
    <dbReference type="GeneID" id="10278812"/>
    <dbReference type="KEGG" id="mel:Metbo_2346"/>
    <dbReference type="eggNOG" id="arCOG04490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114389_2_0_2"/>
    <dbReference type="OMA" id="AAALWYK"/>
    <dbReference type="OrthoDB" id="98606at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PTHR40438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="SSF56271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00286">
        <property type="entry name" value="TIGR00286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_5023532077" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023532076" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="6">
        <location>
            <begin position="38"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="6">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="15702" checksum="68C41BA9D2E6E8CE" modified="2011-05-03" version="1">MRISITSGRAEGPTKLNTFDNALLNAGIGDVNLIKVSSILPKDAEIVPVPKLTPGDMINSVLSYVSSDNEGDIISAFVAVAIADEFGCVVENTGINRNPDDVKAEAEEMARYMVDVRGMKLKEIIIEGKSHIVKKQGAVVAAVVYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T653</accession>
    <name>F0T653_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2347"/>
    <dbReference type="EnsemblBacteria" id="ADZ10560">
        <property type="protein sequence ID" value="ADZ10560"/>
        <property type="gene ID" value="Metbo_2347"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2347"/>
    <dbReference type="HOGENOM" id="CLU_3057129_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="6316" checksum="93E9441946325AFA" modified="2011-05-03" version="1">MLSFKIIFKNTINHNIKKKLISLSIVLIKKVYISKNRYDYDNVDAILNNLKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T668</accession>
    <name>F0T668_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645926.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2362"/>
    <dbReference type="EnsemblBacteria" id="ADZ10575">
        <property type="protein sequence ID" value="ADZ10575"/>
        <property type="gene ID" value="Metbo_2362"/>
    </dbReference>
    <dbReference type="GeneID" id="10278828"/>
    <dbReference type="KEGG" id="mel:Metbo_2362"/>
    <dbReference type="eggNOG" id="arCOG02197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103619_0_0_2"/>
    <dbReference type="OMA" id="GEMEICA"/>
    <dbReference type="OrthoDB" id="104653at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="SSF143870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="173" mass="19260" checksum="19D0D560359B275C" modified="2011-05-03" version="1">MNNKTSEEIDTKRIQIAGFKSDLTNFKAVMEELKLNYSGCVIQLMDADAVAGVQHVLNSTVHAIKSFSRNENIASDMGLEICVRTSGQRQISQAIKMLGIRNGKMNICAVAMDCKSDIMEDLVNILGLRDDHVLEPDTDKLRSIYKISDTQLKTNPDITKILMEKTALLIIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B4</accession>
    <name>F0T6B4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MoaD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644780.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1185"/>
    <dbReference type="EnsemblBacteria" id="ADZ09429">
        <property type="protein sequence ID" value="ADZ09429"/>
        <property type="gene ID" value="Metbo_1185"/>
    </dbReference>
    <dbReference type="GeneID" id="10277635"/>
    <dbReference type="KEGG" id="mel:Metbo_1185"/>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_2"/>
    <dbReference type="OMA" id="NGKAIEW"/>
    <dbReference type="OrthoDB" id="119587at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="93" mass="10496" checksum="F06B4354C3516F53" modified="2011-05-03" version="1">MQKVEVKFLTRFIDITGEKTTTIEDAGDISQLIEALCEKYDETFRDVLLDEDGTVRDYLKVMVNGEDIRDLDGTETKLNDGDEIVMFQTIAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B9</accession>
    <name>F0T6B9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644785.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09434">
        <property type="protein sequence ID" value="ADZ09434"/>
        <property type="gene ID" value="Metbo_1190"/>
    </dbReference>
    <dbReference type="GeneID" id="10277640"/>
    <dbReference type="KEGG" id="mel:Metbo_1190"/>
    <dbReference type="eggNOG" id="arCOG06479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078975_0_0_2"/>
    <dbReference type="OMA" id="MHPRELH"/>
    <dbReference type="OrthoDB" id="100939at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="24460" checksum="BFE40623C4DA3784" modified="2011-05-03" version="1" precursor="true">MKGITKILLVLIVCILIAAAFFQVYRTNVGSNSTGYVTKQVDAYPLPHQTKIAVITGMHPRELHATDVVPKAALAYALQNNVEIDNYQVTVTDEPRDFNVGRANGQALVAGFVNPDIIRSNYSMVIICHDHESGYGSGYYFATPTHDSKSVALGNKVASLLPNFNYYSGSSSASYEATSISQVDLPLTNAGIPVFVYEIPENDDNNTAYSMTYDLLNASFKALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D9</accession>
    <name>F0T6D9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644805.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1211"/>
    <dbReference type="EnsemblBacteria" id="ADZ09454">
        <property type="protein sequence ID" value="ADZ09454"/>
        <property type="gene ID" value="Metbo_1211"/>
    </dbReference>
    <dbReference type="GeneID" id="10277662"/>
    <dbReference type="KEGG" id="mel:Metbo_1211"/>
    <dbReference type="eggNOG" id="arCOG00518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118461_1_0_2"/>
    <dbReference type="OMA" id="PIGFARP"/>
    <dbReference type="OrthoDB" id="97005at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="131" mass="14776" checksum="155529E058C61F23" modified="2011-05-03" version="1">MSMTNEMMDAIEKDLVFLATATEDGIPNVVPIGFARPLDEDTILIADNYMNKSRKNLEKNPWISLVTKDSQKNPFQFKGKVEIFESGKYFDTVTEWGQNAMTKLTPKAAILMKVEEIYSIQPGPEAGKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H2</accession>
    <name>F0T6H2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusA</name>
        <name evidence="7" type="ordered locus">Metbo_2393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645957.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2393"/>
    <dbReference type="EnsemblBacteria" id="ADZ10606">
        <property type="protein sequence ID" value="ADZ10606"/>
        <property type="gene ID" value="Metbo_2393"/>
    </dbReference>
    <dbReference type="GeneID" id="10278860"/>
    <dbReference type="KEGG" id="mel:Metbo_2393"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_0_0_2"/>
    <dbReference type="OMA" id="TDQIKYI"/>
    <dbReference type="OrthoDB" id="99842at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH" evidence="6">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15936" checksum="870B56244D966905" modified="2011-05-03" version="1">MTVKFSTNEIRYIALFESMTGAMVKDLLVDEENGKLTFLVKKGDMGLAIGKRGSTVTKVQKTVDKGVEVIEHSDDPVEFITNLMAPAKLRSIRILQKENGEKIATIEADSRNKRTAIGKAGQNIERARLLAKRQHNINNIIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K9</accession>
    <name>F0T6K9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mreB</name>
        <name evidence="2" type="ordered locus">Metbo_2430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645994.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2430"/>
    <dbReference type="EnsemblBacteria" id="ADZ10643">
        <property type="protein sequence ID" value="ADZ10643"/>
        <property type="gene ID" value="Metbo_2430"/>
    </dbReference>
    <dbReference type="GeneID" id="10278902"/>
    <dbReference type="KEGG" id="mel:Metbo_2430"/>
    <dbReference type="eggNOG" id="arCOG03061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_2"/>
    <dbReference type="OMA" id="MVICIPS"/>
    <dbReference type="OrthoDB" id="43317at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="357" mass="37742" checksum="220E78303D62F44E" modified="2011-05-03" version="1">MFDFLKKNDEVDVKKKALTDTLGIDLGTLNTVVAKPSGDKFDLYKIPSVVAVKKEDPAYVLAVGEEAKSMLGRTPEDIIAVRPLRKGVIESIAQAEALLVYAIDKGSGEKNASIDRIVVGIPGDASEVEKRAVEEIGIKAGANYVLVISEGLSAAIGAGLPIAEAAGTMVIDIGAGSSDIVVISLGGITDIETIRDGGDDIDKNIVEKVKELYNVEIGIHEAEKAKIAVGMVHSDADVENLINTAIGKSMETNKPKQVEIDSKIVADAAEPIVKNLIEALAVVLERMSPELISGVYNKTVVVGGTSQLRGLKERIYQEVGVPVEISDDPMTVVAKGAAIVAAEPRALEPEVRLKAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L1</accession>
    <name>F0T6L1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MotA/TolQ/ExbB proton channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645996.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2432"/>
    <dbReference type="EnsemblBacteria" id="ADZ10645">
        <property type="protein sequence ID" value="ADZ10645"/>
        <property type="gene ID" value="Metbo_2432"/>
    </dbReference>
    <dbReference type="GeneID" id="10278904"/>
    <dbReference type="KEGG" id="mel:Metbo_2432"/>
    <dbReference type="eggNOG" id="arCOG03209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_996096_0_0_2"/>
    <dbReference type="OMA" id="AYIGYNH"/>
    <dbReference type="OrthoDB" id="51457at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="82"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="31070" checksum="6598EF0CC111AA98" modified="2011-05-03" version="1">MIIEFFTGSFATILEMFKSGGIITYIITIIGIYGVILSFEKILYLRKVSKVSLPQIMSTVNDSMERGGSLEALRSLGKYQNPISKITSEALKIGYRNNVEVEDAMERVFIVEMGNMTKGLNSLKTIIEIAPLLGLIGTVIGIWVTFKAMGISANTSQMAEGIYIALITTIAGLTVAIVILPMYSYINSKIEEEIDKIDIAKKMTNWRTAEIRIKVDSCIENAIEALQESNGVIEVRHISDPEANIWISIDPNRLDRSINNIVKEKCNLDPQIIESKLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P0</accession>
    <name>F0T6P0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arsenite-activated ATPase ArsA</fullName>
            <ecNumber evidence="2">3.6.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643624.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0020"/>
    <dbReference type="EnsemblBacteria" id="ADZ08273">
        <property type="protein sequence ID" value="ADZ08273"/>
        <property type="gene ID" value="Metbo_0020"/>
    </dbReference>
    <dbReference type="GeneID" id="10276442"/>
    <dbReference type="KEGG" id="mel:Metbo_0020"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_2"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="OrthoDB" id="84406at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="Anion-transp_ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="PTHR10803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA_ATPase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="332" mass="37535" checksum="7D5E4828EDFDC1A7" modified="2011-05-03" version="1">MAFKDIFKFNKGKTTFVFIGGKGGVGKTTISAATALWFARQGKHTLIISTDPAHSLSDSYERNIGHNPTPIAENLEALEIDPEIAMQDYQAKMQEQQALNPGMDMGMMQDQMDMASMAPGIDEAAAFDKFLQYMMTDEYDIVIFDTAPTGHTLRLLSMPEMMDSWVGKMITIRRQVGSMAKAFKNIMPFMGDEEEEDKALEDMEETKKRIKEARGIMADPMRTTFKTVVIPEEMSIYESERAMQALKKYNMTTDGVIVNQIQPEEADCEFCAARRKIQEQRLKTIQEKFGQQVIAEIPLQKHEVKGMERLTEIGDILYGDHLDDGVKAIPMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q6</accession>
    <name>F0T6Q6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name evidence="4" type="ordered locus">Metbo_0036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643640.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0036"/>
    <dbReference type="EnsemblBacteria" id="ADZ08289">
        <property type="protein sequence ID" value="ADZ08289"/>
        <property type="gene ID" value="Metbo_0036"/>
    </dbReference>
    <dbReference type="GeneID" id="10276458"/>
    <dbReference type="KEGG" id="mel:Metbo_0036"/>
    <dbReference type="eggNOG" id="arCOG04115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052299_1_0_2"/>
    <dbReference type="OMA" id="YVEVKGC"/>
    <dbReference type="OrthoDB" id="97928at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="PTHR30545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SfsA_N" evidence="3">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="SfsA" evidence="2">
        <location>
            <begin position="84"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="238" mass="27173" checksum="60322518FCA4FEBD" modified="2011-05-03" version="1">MKIENIFEGIFVERPNRFLVHFKLNSADKTVEMAHLRDPGRLTELLTPNIKLLLRKATNPNRKTGYDVIAVHNNNRWILINSGFHSDLASELIKSGLIRDINDYSIDRREFSYGNSRIDFLLKAATSIPMLLEVKGCTLVEEGLAKFPDAPTLRGKKHLDELSKSTKNGYEAAVLFLIIRDDAEEFTPNTPMDPDFAASLKNADENRVKIIAYSFQNIYKNNILEIRPFKRVPIVLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T4</accession>
    <name>F0T6T4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0064</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643668.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0064"/>
    <dbReference type="EnsemblBacteria" id="ADZ08317">
        <property type="protein sequence ID" value="ADZ08317"/>
        <property type="gene ID" value="Metbo_0064"/>
    </dbReference>
    <dbReference type="GeneID" id="10276487"/>
    <dbReference type="KEGG" id="mel:Metbo_0064"/>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_0_2"/>
    <dbReference type="OMA" id="ESKTRHR"/>
    <dbReference type="OrthoDB" id="103133at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="143"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="251" mass="28083" checksum="DFD0BD5DF3D3EFC5" modified="2011-05-03" version="1">MTPVDILVVEDEGITAKDIKNSLEKAYYNVIAVVNNGEDAVKFSQEKKPDLVIMDIKLEGKLDGVDAAQIIESKFGIPIIYLTSYSDKNTIERVKSTHPSAFIVKEPFEFLHKPFEENELYTAIDIILYKNKGVSESYNAKGHYKLLESVLKTVSEGAIATDLKGRIIFINPVAANFVEISNESFIGTSVGELIPDIDLNDIIGRFNSTEVIEQNFSIKSNDKNYTSMECTFNPILNELDILDGVIIMFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T6</accession>
    <name>F0T6T6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Isopropylmalate/citramalate/homocitrate synthase</fullName>
            <ecNumber evidence="7">2.3.1.182</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0066</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643670.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0066"/>
    <dbReference type="EnsemblBacteria" id="ADZ08319">
        <property type="protein sequence ID" value="ADZ08319"/>
        <property type="gene ID" value="Metbo_0066"/>
    </dbReference>
    <dbReference type="GeneID" id="10276489"/>
    <dbReference type="KEGG" id="mel:Metbo_0066"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="6">
        <location>
            <begin position="16"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="505" mass="54685" checksum="FBD26C9A9F71E424" modified="2011-05-03" version="1">MYIETVKQTMNLPDTVKILDTTLRDGEQTPGVALTVDEKIRIAKKLDKLGVNTMEVGFPAASDGEVRATKEILKLELDSRVCGLARPLRVDLDAAIDCGVDYIHTFIGTSPLHREYKLKMDKEEILNKSVEGVEYIKDHGITAEFSAEDATRTEFDYLKEVYLAVEDAGADFINVPDTVGVMIPSSMNYLITELKKILKTPISVHCHDDFGLAVANSLSAVEAGASQVHSTINGLGERAGNASLEEVVMALTSQYNIKTSIDTKLLVDTSEFVSRITGIKMPPNKAIIGENAFAHEAGIHVHGVLQNAETYEPITPEMVGHTRRIVLGKHTGANAIKAKLDEYGIELNEDQFIRVFDQVKRLGDKGKCVTDADLKAMSETVLGKAHKEIVKLEGFSVMTGDNVMPTATVKLDINGKVKTAAKTGVGPVDAAINAIQSLVGETADIELKEYNIEAITGGTNALAEVFVIMGDKKGNKATGRSTTDDVVMASVEAVLDSINKILIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U0</accession>
    <name>F0T6U0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ordered locus">Metbo_0070</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643674.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0070"/>
    <dbReference type="EnsemblBacteria" id="ADZ08323">
        <property type="protein sequence ID" value="ADZ08323"/>
        <property type="gene ID" value="Metbo_0070"/>
    </dbReference>
    <dbReference type="GeneID" id="10276493"/>
    <dbReference type="KEGG" id="mel:Metbo_0070"/>
    <dbReference type="eggNOG" id="arCOG01432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_2"/>
    <dbReference type="OMA" id="QWGMAVS"/>
    <dbReference type="OrthoDB" id="24741at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="PTHR48077:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="74"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ08323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="430" mass="47295" checksum="85410ED46A2A095E" modified="2011-05-03" version="1">MYKITLSEKETPKKWYNIAADLPVELPPSTQTEEGHQIDNLPKLFSKYVLQQEMSTERWIDIPKEVRKVYKQIGRPSALFRATGLEKYLDTPAKIYYKREDQSPVGSHKLNTAIAQAYYAKKAGAERLTTETGAGQWGSALSLACSLLGLDCTVYMVNVSFKQKPYRKTVMHLYDGEVLPSPSNRTEFGRKILAEDPNHPGSLGIAISEAMEDAFNDDSAFYTLGSVLNHVLLHQTVIGLEAQKQFAKIDEKPDILVGCVGGGSNFGGAAFPFMKDKLSGDLDCQFLAAEPASCPTLTQGEYKYDYGDTAGLTPLMKMYTLGHEFVPPSVHAGGLRYHGMAPLVALLVNQGLVEGRTVNQTELFQAGKLFANTEGVIPAPETCHAIKVAIDEANKCKKTGEEKNIMITFSGHGLLDLQGYDDFIEGHLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U3</accession>
    <name>F0T6U3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644814.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09463">
        <property type="protein sequence ID" value="ADZ09463"/>
        <property type="gene ID" value="Metbo_1221"/>
    </dbReference>
    <dbReference type="GeneID" id="10277672"/>
    <dbReference type="KEGG" id="mel:Metbo_1221"/>
    <dbReference type="eggNOG" id="arCOG02644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_1_2"/>
    <dbReference type="OMA" id="RATIYLY"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="26052" checksum="BA1052DCEBCF6EB5" modified="2011-05-03" version="1">MAIPERKQREKIQRRESMIDAAEILFFKKGYDNVTMKDIADEVELNRATIYLYFENKEALCFAVILKGMRILNGMIKKNVKRTTARRMINAVGSTYYMFFQMYPQYFKVYNYFQSGRFEVEDLLSSTNGQHKSSVWDLKEILKLQREIFNILLEIITNLDKKTISVDMDPKLLTIIILSTLDGMISPSPVMKMEFKELNLNEYQNFNIIFLNFVNKLLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U6</accession>
    <name>F0T6U6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644817.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1224"/>
    <dbReference type="EnsemblBacteria" id="ADZ09466">
        <property type="protein sequence ID" value="ADZ09466"/>
        <property type="gene ID" value="Metbo_1224"/>
    </dbReference>
    <dbReference type="GeneID" id="10277675"/>
    <dbReference type="KEGG" id="mel:Metbo_1224"/>
    <dbReference type="HOGENOM" id="CLU_1727257_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="17104" checksum="57217E805B15A7B9" modified="2011-05-03" version="1">MLNKKMGYIGAAIFVALVIFTLVYHPHVDTNVFENKYVEFKLPNNIKVVDESNETCDLYFFNSTPGTGDKSDPNFIADMRTVKTNYINNASNITDVTLVDGINATEFVDKSSNTFNLFVPVKKRNNALLFEIFLNQSQVTYETVKNSLIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y4</accession>
    <name>F0T6Y4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644855.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1262"/>
    <dbReference type="EnsemblBacteria" id="ADZ09504">
        <property type="protein sequence ID" value="ADZ09504"/>
        <property type="gene ID" value="Metbo_1262"/>
    </dbReference>
    <dbReference type="GeneID" id="10277713"/>
    <dbReference type="KEGG" id="mel:Metbo_1262"/>
    <dbReference type="eggNOG" id="arCOG06477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074023_0_0_2"/>
    <dbReference type="OMA" id="WNGRETL"/>
    <dbReference type="OrthoDB" id="88447at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="28260" checksum="1B8EF367071ECF38" modified="2011-05-03" version="1">MVAFRRDAIFPAELFIAQDNFYGKNAEIQFKVCGGLFFHTVITSDGWIIASGGFGTSSNQKLMTLASQIMNNGSITQELLENTKPILSNMGLGHFLIKSPDNYVAFEIYFDGTPLNKFFKMNNGEFISVPNDPQYYREGLYSEFHSNPLTAAAEIEGTDLWGVNRRNVILHDVEKNNDSTLLKIWAAYDDGSLLERNEGKGGPDNIRFLDDLIINGKDLPIIPSMVKIGEINLLNQEEPDNSKTAIKAIKNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y5</accession>
    <name>F0T6Y5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="3">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pdxS</name>
        <name evidence="6" type="ordered locus">Metbo_1263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644856.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1263"/>
    <dbReference type="EnsemblBacteria" id="ADZ09505">
        <property type="protein sequence ID" value="ADZ09505"/>
        <property type="gene ID" value="Metbo_1263"/>
    </dbReference>
    <dbReference type="GeneID" id="10277714"/>
    <dbReference type="KEGG" id="mel:Metbo_1263"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="OMA" id="RYANRGW"/>
    <dbReference type="OrthoDB" id="64452at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PTHR31829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00343">
        <property type="entry name" value="TIGR00343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="SOR_SNZ" evidence="5">
        <location>
            <begin position="7"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribose 5-phosphate binding" evidence="3">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="31387" checksum="3F5013F5344279C2" modified="2011-05-03" version="1">MLHGTDVLKKGFAKMTKGGVIMDVVNAEQAAIAEEAGAVSVMALEKVPADIRAEGGVARMADPSKVREIIDAVSIPVMAKVRIGHFVEAQVLQSLGVDMIDESEVLTPADEKYHIDKKNFTIPFVCGARNLGEALRRIDEGAAMIRTKGEAGTGNIVEAVRHSRQISSTIRELQNKTEEELWSVAREIESPLELVKLTAKEGRLPVVNFAAGGVATPADAALMMQLGSDGVFVGSGIFKSEQPSLVAKAIVEATNNFKDAELIAEVSTDLGKAMPGLEISTIPEEEKLQKRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T724</accession>
    <name>F0T724_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646009.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2445"/>
    <dbReference type="EnsemblBacteria" id="ADZ10658">
        <property type="protein sequence ID" value="ADZ10658"/>
        <property type="gene ID" value="Metbo_2445"/>
    </dbReference>
    <dbReference type="GeneID" id="10278917"/>
    <dbReference type="KEGG" id="mel:Metbo_2445"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="OMA" id="HIHISGI"/>
    <dbReference type="OrthoDB" id="51470at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="278" mass="31150" checksum="9803DE6AA0245AE5" modified="2011-05-03" version="1">MKVRFGPAGRPINFSGNAKDACAYIKAEGLDAYEYQATYGVKISQSSAKDLKSSSKSENVLVSMHAPYYINLSSNKDDVIERSIQRLVQSAKAAEWMGAYRIVFHPGFYTTYTPEEALIRCKNAVTDILDELDSLGVKDFCFAPETTGKKSQLGNLDEIIELCRSFDKFQPTVDFAHVHARNGGGIASRDDYAGIFEKLENELGINCLHSHFTHIEYTDAGERKHHILGDHNYGPPLKPFIEEIIECGWKVTVICESPLIDQDAIVMKDLYKDLVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T746</accession>
    <name>F0T746_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="10">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="10">HMP-P synthase</shortName>
            <shortName evidence="10">HMP-phosphate synthase</shortName>
            <shortName evidence="10">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thiC</name>
        <name evidence="11" type="ordered locus">Metbo_2467</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646031.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2467"/>
    <dbReference type="EnsemblBacteria" id="ADZ10680">
        <property type="protein sequence ID" value="ADZ10680"/>
        <property type="gene ID" value="Metbo_2467"/>
    </dbReference>
    <dbReference type="GeneID" id="10278939"/>
    <dbReference type="KEGG" id="mel:Metbo_2467"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="25235at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="424" mass="45929" checksum="80C47175B363B860" modified="2011-05-03" version="1">MTQMQDALRGNITPEMKLVAESEGIDVQKIVRGLSDGRIIIPKNVEGKTKAVAIGKGLKTKINANVGSSSELEKVKWEVEKAKAAVKYGADTIMDLSTGPDYQNVLNAIMEAVNVPIGTVPIYEAGITASNSKGAVVNMDEDDMFNAITNQAKAGVDFITVHCGITKDLIGKVQDSNRIMGIVSRGGSFLAAWILQNELENPLYKNYDYLLEIASEYDVTLSLGDGLRPGCLHDASDVSQIQELVTLGELVTRARNEGVQVMVEGPGHVPINQIKANIEIQKTVCKEAPFYVLGPLVTDLAPGYDHITSAIGGAIAASAGADFLCYVTPAEHLAIPNIDDVKEGVIASKIAAEAADVAKGVKGAWDREVEMANARKNFDWEKQFELAFDQEKPRKCRERKPISEDDMCTMCGEFCALRLVRNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T786</accession>
    <name>F0T786_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646071.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2507"/>
    <dbReference type="EnsemblBacteria" id="ADZ10720">
        <property type="protein sequence ID" value="ADZ10720"/>
        <property type="gene ID" value="Metbo_2507"/>
    </dbReference>
    <dbReference type="GeneID" id="10278981"/>
    <dbReference type="KEGG" id="mel:Metbo_2507"/>
    <dbReference type="eggNOG" id="arCOG05038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340162_0_0_2"/>
    <dbReference type="OMA" id="HYVDENE"/>
    <dbReference type="OrthoDB" id="109265at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="11801" checksum="A990F70AB1A3B6B2" modified="2011-05-03" version="1">MEKEITLNQDELIEYVKNEVKTYDTLEISYNRIFLPTEVIDIEHYVDENELESLNLVLQVNGELLNDTIQLDLIEIKEEIVEIRHIKNEDDLTVLVVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D5</accession>
    <name>F0T7D5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cobD</name>
        <name evidence="13" type="ordered locus">Metbo_0111</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the CobD/CbiB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643715.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0111"/>
    <dbReference type="EnsemblBacteria" id="ADZ08364">
        <property type="protein sequence ID" value="ADZ08364"/>
        <property type="gene ID" value="Metbo_0111"/>
    </dbReference>
    <dbReference type="GeneID" id="10276536"/>
    <dbReference type="KEGG" id="mel:Metbo_0111"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="OMA" id="NSGYTMA"/>
    <dbReference type="OrthoDB" id="101304at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="309" mass="34938" checksum="67881D79B57A12FA" modified="2011-05-03" version="1">MNLIIIILAAVLIDILFGELPSRIHPVVLIGKLINYLIRSLNNHNTKFTGLIITILTLIIPLIIIYTLLDLSKFNYYIYLLVAAIVLSTTFAIRSLITSVKKIEEDLNEDIEKAKHSISYLVSRETSDLEESDIISAAIETLTENITDSITAPLIYTFIFGILGAVFYRVVNTLDAMMGYKTPKYINTGWYPAKLDDLLNYVPARITGFLMVMAAWILRMNWKNSYKIMVRDARKTPSPNSGYTMAATAGALEIQLEKKGVYQLGEQIHPLRIETISRTILLTKVVVLIFLIISVLCYTILTIIIMNWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I2</accession>
    <name>F0T7I2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644901.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1308"/>
    <dbReference type="EnsemblBacteria" id="ADZ09550">
        <property type="protein sequence ID" value="ADZ09550"/>
        <property type="gene ID" value="Metbo_1308"/>
    </dbReference>
    <dbReference type="GeneID" id="10277759"/>
    <dbReference type="KEGG" id="mel:Metbo_1308"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_4_1_2"/>
    <dbReference type="OMA" id="SSRIWHK"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="96"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="318" mass="36932" checksum="0DAA23D10683698E" modified="2011-05-03" version="1">MNPKVKIIILNWNGWEDTIECLESLYQIDYPNFDVLVVDNDSKDESIDKIHMYLDGKLKVESKFLNYDPDNKPIKYLEYSEDRYPQLDTSDGVDAKNLYILKNNENYGFAEGNNIAIKVALRSNPDYIMLLNNDTVVEHDFLNKLVEVAEEDEQVGVVGPTIYYYDYKGRTDVPSNICGVVNIKHYPGYYDMVDQDPTVKPGNLECDWVSGAAMMIKTKEVPIKYLNNQLFFGNEDIDMCINLKTMGYKIINVHSSRIWHKEGVSRKKRSSAVIKKVKMEIEVNLKFLKLHNKHYYWYLPVYLLQVAALYTKVLVGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M1</accession>
    <name>F0T7M1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1349"/>
    <dbReference type="EnsemblBacteria" id="ADZ09589">
        <property type="protein sequence ID" value="ADZ09589"/>
        <property type="gene ID" value="Metbo_1349"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1349"/>
    <dbReference type="eggNOG" id="arCOG03886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529242_0_0_2"/>
    <dbReference type="OrthoDB" id="119483at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="175" mass="20123" checksum="2A8711F8E1C726C1" modified="2011-05-03" version="1">MGDWLKRITISIESELDMKFRKKASQKYQFERGWYSIAVAEAMTYWAEGKDQEIASNKSGPNTPLKDHLDQAMLDKINSELNLEDENLFENFESLINHINNDTQHKLLIEREKGNIVIKMGNDNVSDLKTNLESFMFLYRSLGVILSALEETSKEKFGIVGMGEIPPVYIKKITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N6</accession>
    <name>F0T7N6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644955.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09604">
        <property type="protein sequence ID" value="ADZ09604"/>
        <property type="gene ID" value="Metbo_1364"/>
    </dbReference>
    <dbReference type="GeneID" id="10277815"/>
    <dbReference type="KEGG" id="mel:Metbo_1364"/>
    <dbReference type="HOGENOM" id="CLU_2678948_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="74" mass="8639" checksum="F9A7256F9087E167" modified="2011-05-03" version="1">MSYGENEGEKRKKLKTKPKSDEVLDEESEMFPDAEEERMKVAPADLLEKERPDLLEKEYGKNVKVNPQKNKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N8</accession>
    <name>F0T7N8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="6">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="6">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="6">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aspS</name>
        <name evidence="8" type="ordered locus">Metbo_1366</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644957.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1366"/>
    <dbReference type="EnsemblBacteria" id="ADZ09606">
        <property type="protein sequence ID" value="ADZ09606"/>
        <property type="gene ID" value="Metbo_1366"/>
    </dbReference>
    <dbReference type="GeneID" id="10277817"/>
    <dbReference type="KEGG" id="mel:Metbo_1366"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="OrthoDB" id="24288at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="PTHR43450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="7">
        <location>
            <begin position="144"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="6">
        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="410"/>
            <end position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="439" mass="50213" checksum="66C14ED878120781" modified="2011-05-03" version="1">MTDLLEDCRRTHYSKQIKPEMKDEDVVIMGWIHEMRDLGGIIFVLLRDRDGVTQVTAPSKKIEPELFEELKKLKKESVIAVKGRVQESAKAPGGVEIIPETIKLLSESKLPLPLDTTEKVRAEIDTRLDSRFIDLRKHSVSAIFKVKSRMLHSVRNFLESENYTEINTPKLVGSATEGGTELFPITYFEREAFLGQSPQLYKQMMMASGFDNVYEIAPIFRAEEHDTLRHLNEVISIDVETAFTDQIDAMNILEKMVVKAITDVKEHCKDALDTLEFDLQIPETPFPRIEYDEMVEMVNNKGVKMEHGEDMSRAAEKVMGELMDGYYFITAWPTDIKPFYVQPSAEDPAKSCAFDLMYKDLEISSGAMRIHNHDLLVEKIKSKGLNPDSFNRYLAAFEYGMPPHAGWGVGAERFTMTMTGQTNIRETVLFPRDRRRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U7</accession>
    <name>F0T7U7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643785.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0182"/>
    <dbReference type="EnsemblBacteria" id="ADZ08434">
        <property type="protein sequence ID" value="ADZ08434"/>
        <property type="gene ID" value="Metbo_0182"/>
    </dbReference>
    <dbReference type="GeneID" id="10276608"/>
    <dbReference type="KEGG" id="mel:Metbo_0182"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="OMA" id="IYYGTKF"/>
    <dbReference type="OrthoDB" id="120594at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF35_N" evidence="2">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="OB_aCoA_assoc" evidence="1">
        <location>
            <begin position="50"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="15063" checksum="44CB0CF0107A4DB1" modified="2011-05-03" version="1">MKDIVRGWRHIQQRYNLIGSKCSQCGNVFFPSRVLCPECRRKGKIEEFKFSGDGKIHTFSVIHTPTDEFKTLAPYVVAIVELEEGAKITSQIVDCDPDAIEIGDEVEMVFRKIKEEGDDGVISYGYKFKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U9</accession>
    <name>F0T7U9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643787.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0184"/>
    <dbReference type="EnsemblBacteria" id="ADZ08436">
        <property type="protein sequence ID" value="ADZ08436"/>
        <property type="gene ID" value="Metbo_0184"/>
    </dbReference>
    <dbReference type="GeneID" id="10276610"/>
    <dbReference type="KEGG" id="mel:Metbo_0184"/>
    <dbReference type="eggNOG" id="arCOG07613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876067_0_0_2"/>
    <dbReference type="OMA" id="GIMANIT"/>
    <dbReference type="OrthoDB" id="49811at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="317" mass="35540" checksum="11F5CD2ECA87FA36" modified="2011-05-03" version="1">MDHKGFIFSLDVFLSLLIMILILGISADAMDIAGNRIDNFYSEQSHQRIADDACDILIKTSGSPDYWEEMRSASGTSPGLADTNNSSGTNKLSSKKLEALKNNPELMDKLIPDGFKCSVTIYPFDSSLPILSIVNQTNSFNTEIYVANRTIVYNYNSYVIYSKLSIEQSLEANKSHYKCPHSEINHYAHDLPDFNDKIPGWVCDVFKIGQDTLNSTDFYLMTDPPHILDQGALWMIDSPNNLSATSERFEACPMDVTRRVRDLGKDGVLILHIHTSADRNMPFKVYILGVPSGTPVSDVKINYLGLKSGYFVLKIWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W4</accession>
    <name>F0T7W4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">csl4</name>
        <name evidence="6" type="ordered locus">Metbo_0199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643802.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0199"/>
    <dbReference type="EnsemblBacteria" id="ADZ08451">
        <property type="protein sequence ID" value="ADZ08451"/>
        <property type="gene ID" value="Metbo_0199"/>
    </dbReference>
    <dbReference type="GeneID" id="10276625"/>
    <dbReference type="KEGG" id="mel:Metbo_0199"/>
    <dbReference type="eggNOG" id="arCOG00676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_1_1_2"/>
    <dbReference type="OMA" id="CGNVETR"/>
    <dbReference type="OrthoDB" id="111542at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="66"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0204"/>
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        <property type="gene ID" value="Metbo_0204"/>
    </dbReference>
    <dbReference type="GeneID" id="24964514"/>
    <dbReference type="KEGG" id="mel:Metbo_0204"/>
    <dbReference type="eggNOG" id="arCOG11270">
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    </dbReference>
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    <dbReference type="OrthoDB" id="117285at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
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    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23414" checksum="361ED166BDEB101B" modified="2011-05-03" version="1">MVEDSQRKEEESELTADSSGNNVDGSETVSEVSSKASDSEPKNGSDGDTQTKSSIIEKQDDIKSGSDQTPNHDKAADHHEDHAINFHEIMGHDLFKSIRSRKDQILRYVAVAIGVILIIYGVVLISASVTKVADNVIFGEGASFAAFSMLLGFLIIVGAFSQKILNKTFLKNINSELEIAEGKTEAADEKTEDISGKKDPNKVEDGKDNIVGEDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T852</accession>
    <name>F0T852_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09678">
        <property type="protein sequence ID" value="ADZ09678"/>
        <property type="gene ID" value="Metbo_1440"/>
    </dbReference>
    <dbReference type="GeneID" id="10277891"/>
    <dbReference type="KEGG" id="mel:Metbo_1440"/>
    <dbReference type="eggNOG" id="arCOG02008">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112363_0_0_2"/>
    <dbReference type="OMA" id="LWNIMGA"/>
    <dbReference type="OrthoDB" id="135174at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="22631" checksum="62A44341FFF38373" modified="2011-05-03" version="1">MKKIYPLIGILGPIIYILAVFVGGALRHDYSALYNAISELSMANAPNKLLMDILFGLYNVFILIFGVGAFLDPDFKSKKFKSGALMLFVIGVLGLMVLVFTQDPRGTPATLFGTLHIALSGVTAALTIISVIVIGLSFKDYVGMKSFSWYSYLTAIFIFLSGGAGAASIAVNSPFGGLFERITIFLFMVWVIVFSYILYKGNLKPVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T864</accession>
    <name>F0T864_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645041.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1453"/>
    <dbReference type="EnsemblBacteria" id="ADZ09690">
        <property type="protein sequence ID" value="ADZ09690"/>
        <property type="gene ID" value="Metbo_1453"/>
    </dbReference>
    <dbReference type="GeneID" id="10277904"/>
    <dbReference type="KEGG" id="mel:Metbo_1453"/>
    <dbReference type="eggNOG" id="arCOG02232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="OrthoDB" id="43345at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="399" mass="42788" checksum="9E2ECBCFD7DD31AE" modified="2011-05-03" version="1">MNVLVVGSGAAGGTVARELSKNGISVTIIEKGPAIKVKNAYKEYIIQNVGTEISNTVCLGGTTLVTLGNGMKDCADDFKEFGIDLNPEFEEIERELFVSHLPDSHFGDGTKRIMESAESIGFEVEKMPKFIDPTSCKPCGKCALGCPQDAKWTSMTFIEEAEKYGARIIENTPVTDIIVSEGKVRGVKSYEEIFEADIVILAAGAIETPRLLQKIGMNAGNNLFVDTFVTVGGILENIKFNTELQMNAIIKFDNFIIGPHFTEVLVNDLNAHNARKKDVIGLMVMIKDEPSGKVTPDEIIKLNTGNDLAKLAQGSAIASSILIEAGVDPKTIVSTHARGAHLGGTASIGDLVNKNLQTEVEGLYVGDASIFPKAPGAPPVLTIIALAKRLAKHVMEMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T873</accession>
    <name>F0T873_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0233"/>
    <dbReference type="EnsemblBacteria" id="ADZ08485">
        <property type="protein sequence ID" value="ADZ08485"/>
        <property type="gene ID" value="Metbo_0233"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0233"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081793_1_0_2"/>
    <dbReference type="OMA" id="NEMIDPH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="231" mass="26021" checksum="0CAF667947636DF3" modified="2011-05-03" version="1">MLDEWLDEFSRNLGADYYGVADITNAGDFIFQQGKINVEQYPRAISIGILLLNTIVDDLPRRSEHSVAVNYHHTYNIVNLQLDLIASRLARSIQKQGYTALPVPASERYDDEMICAVFSHKLAANLAGLGWIGKSCLLITPENGPRVRWTTILTNVPLTPTGKPMKNLCGDCRKCVDICPVKAFTGSNFKEDEDRDVRYDARKCELYLENLEKQEKLPVCGLCIYNCPFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A6</accession>
    <name>F0T8A6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643869.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0266"/>
    <dbReference type="EnsemblBacteria" id="ADZ08518">
        <property type="protein sequence ID" value="ADZ08518"/>
        <property type="gene ID" value="Metbo_0266"/>
    </dbReference>
    <dbReference type="GeneID" id="10276698"/>
    <dbReference type="KEGG" id="mel:Metbo_0266"/>
    <dbReference type="eggNOG" id="arCOG05076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173001_0_0_2"/>
    <dbReference type="OMA" id="LVSIFYW"/>
    <dbReference type="OrthoDB" id="130238at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12558" checksum="964B4C4CE04831A9" modified="2011-05-03" version="1">MNIYDFILKKIKDVKMETGDDGPVTNISATSMLTAEITIISSLLIAVVMLRLLNRALMMALVVIVFLIIFMAMPIMPKLKREQNDSLATMSFYVIVALAIIVSLFYWGNLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D5</accession>
    <name>F0T8D5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0295"/>
    <dbReference type="EnsemblBacteria" id="ADZ08547">
        <property type="protein sequence ID" value="ADZ08547"/>
        <property type="gene ID" value="Metbo_0295"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0295"/>
    <dbReference type="eggNOG" id="arCOG00602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_4_0_2"/>
    <dbReference type="OMA" id="RQLMRDY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="157"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="225"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="289" mass="32055" checksum="A79513C6BD2AC072" modified="2011-05-03" version="1">MCLMVNLFVRSVKKIWMVINLMTVGEIMSSDPVTVNVDTQATKVRSIFREGWFRSIPVLSKNRLEGIISRGDIMYLSSTKSNIEARVIMKRPKLLATPEMDIRDVAKNLIKSDSVQAAVVESTENMRVVGILSMGDILKELIGSESSTDDSTITEIVTKKVVKANYDDHLSKVWALMDESGFSGLPVVKKDKLIGIITRKDIIKSGHAIKGLDNVDKSIKVEKVMVTPPIVVTNESSVKQAAELMLNNDIGRLPVVENPVYIKKEPQRAKESNIIGIIAREDILGTYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D6</accession>
    <name>F0T8D6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643899.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0296"/>
    <dbReference type="EnsemblBacteria" id="ADZ08548">
        <property type="protein sequence ID" value="ADZ08548"/>
        <property type="gene ID" value="Metbo_0296"/>
    </dbReference>
    <dbReference type="GeneID" id="10276728"/>
    <dbReference type="KEGG" id="mel:Metbo_0296"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_0_0_2"/>
    <dbReference type="OMA" id="GSDWAFN"/>
    <dbReference type="OrthoDB" id="67420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="35"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="178"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="262"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="316" mass="34984" checksum="89A67D117FE678F1" modified="2011-05-03" version="1">MRRKETINLVKSRDRGPLEFESHASEHEGDVMSIAKKTVVTAPQTTTIKEAAETMVKNKFRRLPITNPGTGQILGIVTSMDILDFLGGGNKYKILEEKYPGNFLGAINESVKEIMTRDVEVITHKDSIDHAIDIMRKKEIGALPVVDADHKMVGIVSERDFVILLSGVLTDEVVEDYMTKNVIATTPGTRIEGASKIMVRNKLRRIPVVGEERKTSHPEKDKIMGIVTATDILEFLGKNSAFERMISNDAEDVLNTTITEIMEKNVVATTANTRLGDLCTSMEHENIGGLPVVYNGELEGIITERDILKAVSKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D8</accession>
    <name>F0T8D8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643901.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0298"/>
    <dbReference type="EnsemblBacteria" id="ADZ08550">
        <property type="protein sequence ID" value="ADZ08550"/>
        <property type="gene ID" value="Metbo_0298"/>
    </dbReference>
    <dbReference type="GeneID" id="10276730"/>
    <dbReference type="KEGG" id="mel:Metbo_0298"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_2_0_2"/>
    <dbReference type="OMA" id="QDSRIKN"/>
    <dbReference type="OrthoDB" id="67420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="80"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="143"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="217"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="30532" checksum="0F597E89EE3AF056" modified="2011-05-03" version="1">MHVKDIMKEEIVLVDKDQNIPDALKLMKKHKISRLPVVNTNSDHVRELVGMVTEKDIAMRLGSSKYGKLPPSHFHVSTVMEQDPLVVEADQSLGTVAQIMIQEKLDGMPVVSKDEVIGVLTKTSFLEICKGKPYNVTKISDRMQDEIITVSPTDRLVHARRCLIDNGVGRLPVVEDDVLVGILTAKDVANAMISFRKIVPDKYKNSRIRNLLVEDVMTQNVRTIDPESTLEQVSTMMLENRYSGIPVEDEGSLVGIITKTDLIKYIAELEEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G7</accession>
    <name>F0T8G7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645069.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1485"/>
    <dbReference type="EnsemblBacteria" id="ADZ09718">
        <property type="protein sequence ID" value="ADZ09718"/>
        <property type="gene ID" value="Metbo_1485"/>
    </dbReference>
    <dbReference type="GeneID" id="10277936"/>
    <dbReference type="KEGG" id="mel:Metbo_1485"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="11300" checksum="9048A13C75136261" modified="2011-05-03" version="1">MKETVLWLLTGSRGGKNRVRIINLIYKKPLNAYRISEELNLNYNTVNYHLKILKEHKLLYCNKNVKYGALYFVTDNMNSCRETFDEILENLNLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H7</accession>
    <name>F0T8H7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1496"/>
    <dbReference type="EnsemblBacteria" id="ADZ09728">
        <property type="protein sequence ID" value="ADZ09728"/>
        <property type="gene ID" value="Metbo_1496"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1496"/>
    <dbReference type="HOGENOM" id="CLU_1458206_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="21532" checksum="98BE7FCDFC88177A" modified="2011-05-03" version="1">MSNSTEFKTLLGLITIVAVFFVVVINGDSQIYGYALINIFYFFMAVAFVITLVWYLLRNKSKITIRNIKKECYDYINALKNVRKLPDSCRNTVLTNYISLILILVGLILNIIAHLLDGTVNENWVGMLTVIILCIGYLLFFYTLVIMAIKMKKIENKPLLQIHSNWIWRIFIVYLFLNFILTIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M3</accession>
    <name>F0T8M3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643916.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0313"/>
    <dbReference type="EnsemblBacteria" id="ADZ08565">
        <property type="protein sequence ID" value="ADZ08565"/>
        <property type="gene ID" value="Metbo_0313"/>
    </dbReference>
    <dbReference type="GeneID" id="10276750"/>
    <dbReference type="KEGG" id="mel:Metbo_0313"/>
    <dbReference type="eggNOG" id="arCOG00659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833177_0_0_2"/>
    <dbReference type="OMA" id="CKFCYMS"/>
    <dbReference type="OrthoDB" id="41361at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="26"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="37545" checksum="DC8345576663F14D" modified="2011-05-03" version="1">MNLINKIETYDVLDLLQKANKINIENHGNLVTLERAIFLSWYCEKGDCAFCYMSSQKSKIKDPQKARRTVESVLAEAEVTRRMGWNIEFLSGGYGLFKTPEIKTISQEIYSITGKPVWLNLGITRDIEGFGDEIAGITGAVEVANPELHKKICPSKSIADIVDMLELAGDLGFKKAITIILGLGEKPEDLEYVWELIEDLKIHRVTFYSLNPHKDTVYENSPQPASLYYAGVVAATRIKFPALEIVTGTWIDNLANIGPLLLAGANGITKFPLFKMYGTRYGKRVEEEVKWAGKVLQGTFTDINRLECEDFSNETLEPHVNQYIRRCLGKKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N9</accession>
    <name>F0T8N9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="3">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mptA</name>
        <name evidence="4" type="ordered locus">Metbo_0329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + diphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="3">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643932.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0329"/>
    <dbReference type="EnsemblBacteria" id="ADZ08581">
        <property type="protein sequence ID" value="ADZ08581"/>
        <property type="gene ID" value="Metbo_0329"/>
    </dbReference>
    <dbReference type="GeneID" id="10276766"/>
    <dbReference type="KEGG" id="mel:Metbo_0329"/>
    <dbReference type="eggNOG" id="arCOG04301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="OMA" id="PCSQGMS"/>
    <dbReference type="OrthoDB" id="109805at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003933">
        <property type="term" value="F:GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00294">
        <property type="entry name" value="TIGR00294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="May be catalytically important" evidence="3">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="321" mass="36142" checksum="DD004A00C7379135" modified="2011-05-03" version="1">MDKCFPDTQDKIPTIPVHLTRVGVKGVKKLLKIEREEKRPIVLLPTFNAFVDLPSTQKGIHMSRNPEAISEVLEQALEGTTLEIESLCAKIASCLLKKHKYAKRVEVSMKSDYMIMKKSPVTQIKTQEMTNIMADAIGVRNEDGIVIRKMIGAEVVGMTVCPCAQESTKESSKQELLKFLDEETTEKVLETVSFASHNQRGRGMIMMEVPENYVIRAEDLIRIIEDSMSSPVCELLKRSDENAVVVHAHKHPMFVEDCVRNMVQSIANEYSHLPDDALVTVRQTNEESIHRHNAFAEKVATMGELKREINNTEAADIDAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U2</accession>
    <name>F0T8U2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645121.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09770">
        <property type="protein sequence ID" value="ADZ09770"/>
        <property type="gene ID" value="Metbo_1540"/>
    </dbReference>
    <dbReference type="GeneID" id="10277995"/>
    <dbReference type="KEGG" id="mel:Metbo_1540"/>
    <dbReference type="HOGENOM" id="CLU_1551836_0_0_2"/>
    <dbReference type="OrthoDB" id="144557at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030987">
        <property type="entry name" value="AbiV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18728">
        <property type="entry name" value="HEPN_AbiV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04498">
        <property type="entry name" value="AbiV_defense"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="172" mass="20289" checksum="892FE6A038E10C0B" modified="2011-05-03" version="1">MTSKKLPKDVKNHFEKYWKLCIKFFEDSEYQLASFFAITLIEEIGKLSIIRNKSLGGSFDDKGFYNHHKKYIYAAFDNLSNNYRVTRIYSKKESKFAELVLKKKLFDMRNNSLYLEEDMVTPEKVISRDDAFLLVCFAGEIYAEIQGESIGPSPDEWLKILEEVNQFREHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X5</accession>
    <name>F0T8X5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1576</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645154.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1576"/>
    <dbReference type="EnsemblBacteria" id="ADZ09803">
        <property type="protein sequence ID" value="ADZ09803"/>
        <property type="gene ID" value="Metbo_1576"/>
    </dbReference>
    <dbReference type="GeneID" id="10278032"/>
    <dbReference type="KEGG" id="mel:Metbo_1576"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="OMA" id="WINIENT"/>
    <dbReference type="OrthoDB" id="35869at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol_B_exo1" evidence="7">
        <location>
            <begin position="32"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol_B" evidence="6">
        <location>
            <begin position="382"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="579" mass="67100" checksum="EDF6161170C8C57E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y4</accession>
    <name>F0T8Y4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Polyphosphate kinase</fullName>
            <ecNumber evidence="6 7">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-polyphosphate phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Polyphosphoric acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ppk</name>
        <name evidence="12" type="ordered locus">Metbo_1585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="6 7">An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the polyphosphate kinase 1 (PPK1) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645163.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1585"/>
    <dbReference type="EnsemblBacteria" id="ADZ09812">
        <property type="protein sequence ID" value="ADZ09812"/>
        <property type="gene ID" value="Metbo_1585"/>
    </dbReference>
    <dbReference type="GeneID" id="10278042"/>
    <dbReference type="KEGG" id="mel:Metbo_1585"/>
    <dbReference type="eggNOG" id="arCOG04535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009678_3_0_2"/>
    <dbReference type="OMA" id="NIKWARK"/>
    <dbReference type="OrthoDB" id="17467at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009358">
        <property type="term" value="C:polyphosphate kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00347">
        <property type="entry name" value="Polyphosphate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003414">
        <property type="entry name" value="PP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041108">
        <property type="entry name" value="PP_kinase_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024953">
        <property type="entry name" value="PP_kinase_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036830">
        <property type="entry name" value="PP_kinase_middle_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025200">
        <property type="entry name" value="PPK_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025198">
        <property type="entry name" value="PPK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036832">
        <property type="entry name" value="PPK_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30218">
        <property type="entry name" value="PTHR30218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02503">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13090">
        <property type="entry name" value="PP_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17941">
        <property type="entry name" value="PP_kinase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13089">
        <property type="entry name" value="PP_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015589">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140356">
        <property type="entry name" value="SSF140356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03705">
        <property type="entry name" value="poly_P_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 6 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PP_kinase_N" evidence="9">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase" evidence="8">
        <location>
            <begin position="124"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C_1" evidence="11">
        <location>
            <begin position="334"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C" evidence="10">
        <location>
            <begin position="505"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphohistidine intermediate" evidence="6">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="566"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="704" mass="82216" checksum="EE2DCDC195CE21B6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T919</accession>
    <name>F0T919_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643992.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0389"/>
    <dbReference type="EnsemblBacteria" id="ADZ08641">
        <property type="protein sequence ID" value="ADZ08641"/>
        <property type="gene ID" value="Metbo_0389"/>
    </dbReference>
    <dbReference type="GeneID" id="10276826"/>
    <dbReference type="KEGG" id="mel:Metbo_0389"/>
    <dbReference type="eggNOG" id="arCOG03424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="KYTIIEI"/>
    <dbReference type="OrthoDB" id="79499at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13663" checksum="8B90C1F91A718235" modified="2011-05-03" version="1">MKVHLRVFVEIENLAKAMNALTDAGITGFYILEYRGMSPQDWKGFSIKEDPESAIGIIRDYAVDAVLVCSVVDEDLVDSIVKSVEIALKGEKYTILEVPIRRIIVSYGGSKKKIDENQNELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T965</accession>
    <name>F0T965_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">V-type ATP synthase subunit C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">V-ATPase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">atpC</name>
        <name evidence="11" type="ordered locus">Metbo_0435</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644038.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0435"/>
    <dbReference type="EnsemblBacteria" id="ADZ08687">
        <property type="protein sequence ID" value="ADZ08687"/>
        <property type="gene ID" value="Metbo_0435"/>
    </dbReference>
    <dbReference type="GeneID" id="10276872"/>
    <dbReference type="KEGG" id="mel:Metbo_0435"/>
    <dbReference type="eggNOG" id="arCOG02459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059311_0_0_2"/>
    <dbReference type="OMA" id="LRKFDVW"/>
    <dbReference type="OrthoDB" id="75042at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_su_c/d_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_c_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_su_c/d_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_suC/d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="SSF103486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02923">
        <property type="entry name" value="AhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="385" mass="43253" checksum="AA4FEC71F9DF16C7" modified="2011-05-03" version="1">MVEDITTLVTALGFPSYEIFLAVMILAIIVIGAIVVITIIRPVLGFFPYTYPNARVRARTGRLFNEKEFSEIVESQNVEEVKNYLRGVPDYAKYIDTYPLEKALDSQLAETYDLIARITPDDSKDSFKFLLKKWDIKNIKSIIIAKEAGLSQEQTVDLLVPFGELSNKLDSLIDTDSVEEVLNALEGTEYPKIVEDALPIYKETGLLLPLEASMDKYLLENLLKTSATPEDENTTLLHNYIGTMVDVANIKIILRAKADNIKYEDIEPYMVSDGYEIREWKLKELMEAEDVSGVISGLEGTDYAPMLSDAMSEFNETGSMATFEKLLDSYVVKTAKTISLKNQFGIGPMIGFLSRKETEVKNLKIIARGKREAGFSPSMIKEMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T976</accession>
    <name>F0T976_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644049.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0446"/>
    <dbReference type="EnsemblBacteria" id="ADZ08698">
        <property type="protein sequence ID" value="ADZ08698"/>
        <property type="gene ID" value="Metbo_0446"/>
    </dbReference>
    <dbReference type="GeneID" id="10276884"/>
    <dbReference type="KEGG" id="mel:Metbo_0446"/>
    <dbReference type="eggNOG" id="arCOG04219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438122_0_0_2"/>
    <dbReference type="OMA" id="EWKHNEL"/>
    <dbReference type="OrthoDB" id="102053at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="188" mass="21886" checksum="D495524F75857908" modified="2011-05-03" version="1">MVKVVKSAVKCYKKKTKKTVGGKRKVYEYNQYLVPLKRSDNFQCSEDVLIIPRSEMEDLALANKTTVAEFFDNVGYGKNSIAAYERELAELEWKHNELSRSYKELVSKHSKTNKRIRLDGEKITTLENENNAVQREFEALKKDHDELKSRFQLEFQKKKADEEENAQKDDIWSSLKNRLGSKKDGEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B0</accession>
    <name>F0T9B0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645212.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1635"/>
    <dbReference type="EnsemblBacteria" id="ADZ09861">
        <property type="protein sequence ID" value="ADZ09861"/>
        <property type="gene ID" value="Metbo_1635"/>
    </dbReference>
    <dbReference type="GeneID" id="10278092"/>
    <dbReference type="KEGG" id="mel:Metbo_1635"/>
    <dbReference type="eggNOG" id="arCOG02589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451424_0_0_2"/>
    <dbReference type="OMA" id="IIIFDFP"/>
    <dbReference type="OrthoDB" id="87451at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="54"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="21712" checksum="B837AF139634A924" modified="2011-05-03" version="1">MSRKDEWKSVEKILNNVFNEEDTENLVYSMKTSVKHEIEQIVKGFAKKINEPVEILLVGNDPKFMQEIISTFGRSNLITTIRFTGSIEEAHSMIFQQGVYTDFPIANIIIFDFPTLSGKNGIKILEEIMVKDSIIQNVPVFILNDDFEKVKHLAKYHPDLFLTKPDNFEEYKNVIEEIKEFWLTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C7</accession>
    <name>F0T9C7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09878">
        <property type="protein sequence ID" value="ADZ09878"/>
        <property type="gene ID" value="Metbo_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1652"/>
    <dbReference type="HOGENOM" id="CLU_2366217_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10335" checksum="DE8E15DE9D513AFF" modified="2011-05-03" version="1">MDMGSWQVLYASIRKHISGDSLIDLNNNLHMIIGLVGIGGLAVSMLLVGVLMFTLSAFYLRKPMIAGLIGNLSSFTVAIGVILLFIRKDLNKNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D5</accession>
    <name>F0T9D5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Taurine-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1660</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.36" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645237.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1660"/>
    <dbReference type="EnsemblBacteria" id="ADZ09886">
        <property type="protein sequence ID" value="ADZ09886"/>
        <property type="gene ID" value="Metbo_1660"/>
    </dbReference>
    <dbReference type="GeneID" id="10278117"/>
    <dbReference type="KEGG" id="mel:Metbo_1660"/>
    <dbReference type="eggNOG" id="arCOG00193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="YSLFPWR"/>
    <dbReference type="OrthoDB" id="64935at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="28679" checksum="E247F6B9A2F6D5F8" modified="2011-05-03" version="1">MVFKIKKVNKNFQNKTMEMEILKDIDLTIEDGEFLVLLGPSGCGKTTLLRMIAGLDFPTTGTITENDVPVLNPSPERGFVFQQYSLFPWRTVLDNVAFGLEVNGIDEEERYEKAKNYIEMVGLSAFLESYPNQLSGGMKQRVAIARALVNEPNSLLMDEPFAALDVLSRHKLQKEIIQIWEQETKTIIFVTHNVDEAVFLADRILVFSERPGRIIETFNIEQERIRDRTSEEYLAIKRKITGLLETNQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F7</accession>
    <name>F0T9F7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin resistance ABC transporter ATPase subunit</fullName>
            <ecNumber evidence="6">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08705.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ08705">
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        <property type="gene ID" value="Metbo_0453"/>
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    <dbReference type="KEGG" id="mel:Metbo_0453"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="236"/>
        </location>
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    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
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        </location>
        <ligand>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="35207" checksum="DE65C0D290C64829" modified="2011-05-03" version="1">MEYIIETHDISKVYDDFTAVNSINLKVPKNSVYGVLGPNGAGKTTLISMLCTILHPTSGTATVNGYDVMKNSKEVRQSIGVVFQSRALDDILTGREHLEMHAALYGVPMDLRKQRIEEVLDLIALGDKADEYTKTYSGGMKRRLEIGRGLIHHPKVLFLDEPTLGLDPQTRENIWEYIQELNKTEDITVLMSTHYMEEADKLCDEIAIINHGQIITADSPKNLKRDLRADVITVKVDDEEKFINSAEKLDFIKDIIVADSEIKLMVERGENLVATVVNFANKNDIIVKSIELEHPNLEDVFINLTGKRIVDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J2</accession>
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    </organism>
    <reference evidence="4" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ08740">
        <property type="protein sequence ID" value="ADZ08740"/>
        <property type="gene ID" value="Metbo_0488"/>
    </dbReference>
    <dbReference type="GeneID" id="10276928"/>
    <dbReference type="KEGG" id="mel:Metbo_0488"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="OMA" id="WNDQQDS"/>
    <dbReference type="OrthoDB" id="29908at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PFOR_II"/>
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    </dbReference>
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    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="238"/>
        </location>
    </feature>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="382" mass="42267" checksum="5010CF28002C4C1F" modified="2011-05-03" version="1">MVLKVMSSNQAIAEAVKLAKPKVIPVYPITPQTTISEYLAKYVADGDIKAEYIRVESEHSAISACLGASGTGVRVFTATSSQGLALMHEIIFAAAGLRAPIVMADANRALSAPLSIWNDQQDSISERDSGWMQIYAENGQEALDSVLMSYKISENKEVLLPTMVCVDGFILTHTVDPVDVPEEEEVDKFLPDYNPDHAYLDPERPMSIGTFTDPDYYMEARHDMEVAMEKAKTVISDVSQEFAETFGRKYDFIEKYRSEDADTILIAMGSICSTIKDVIDELREEGEKVGLVRVRVFRPFPVEEIYDAIKGASNVGVIDKNFSFSIGGVLHNNIKSMMDVNAAGFIIGLGGRDIKPSHIREVVEKTKNPSNKPLWIGLKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K3</accession>
    <name>F0T9K3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0499"/>
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        <property type="protein sequence ID" value="ADZ08751"/>
        <property type="gene ID" value="Metbo_0499"/>
    </dbReference>
    <dbReference type="GeneID" id="10276939"/>
    <dbReference type="KEGG" id="mel:Metbo_0499"/>
    <dbReference type="eggNOG" id="arCOG10462">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200894_0_0_2"/>
    <dbReference type="OMA" id="IVMTFCR"/>
    <dbReference type="OrthoDB" id="126958at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="7434" checksum="B6C38EE11F4CBB10" modified="2011-05-03" version="1">MKFEEHAQECNEALNELVGKKVLDIKFKPYDNNCWRIYINTDGGKMVMSFCKDWSCPEVESRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N4</accession>
    <name>F0T9N4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="566" mass="59835" checksum="54C43B4E4AFA0625" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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    </evidence>
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    </evidence>
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    <accession>F0T9S3</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <sequence length="109" mass="12365" checksum="0FEEAAFC3198DD0D" modified="2011-05-03" version="1">MTLELIFINRMIILAAVIVGIANVGLLSGLFYFYWNSYHELKSKFTTGLIYFAVILLVQNVLAIMALSLFALLGIEMHERGATEVYFVLLLVETAQLIAFTILFKITWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA36</accession>
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="232" mass="25909" checksum="8A911A0FF54453A6" modified="2011-05-03" version="1">MVELKKQSELFKNLLELKYEPVAVIFTNDQVSTGKYEKTPICRAIKLASEGGCYIVDKETSTCPGGSRYCGFSGTPTMDEKRRLQNFLTRGEKLTSSIVSFERMQKLSVEPPTGLSDRIIICPLSLAEKRPDVVLFLVNPEQACRLVTLDTYWDGLSPDQRIIGALCQTSISYSLMTGNTNLSMGDWTARHHQEFDPDILFLSIPYERMDNLIKAVPKCSAGDASVLLPDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA55</accession>
    <name>F0TA55_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
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    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
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                <person name="Imachi H."/>
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</entry>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
            <begin position="21"/>
            <end position="519"/>
        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
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        </alternativeName>
    </protein>
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        </lineage>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="OMA" id="KGPGIDH"/>
    <dbReference type="OrthoDB" id="98477at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="PTHR21227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="SSF53032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="SSF55267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_int_endo_N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_int_endo" evidence="2">
        <location>
            <begin position="76"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="18991" checksum="7596C8E47840B888" modified="2011-05-03" version="1">MNAELSGDIVIVKNGNTAKIHEKSHFGNSSEDGLQLSFVEALYLMEKGKLEVEADGEEISIEEMFGFIRKTSAFSNYIVYRDLRNRGYIVKTGFKYGSEFRLYDRGKSPGDGHSNYLVKVASENSEFMMSDLSSYVRVAHGVNKKLLFAVVDEENDITYYNVEWTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC3</accession>
    <name>F0TAC3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="5">SDH</shortName>
            <ecNumber evidence="2 5">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aroE</name>
        <name evidence="9" type="ordered locus">Metbo_0624</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="2 5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644226.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0624"/>
    <dbReference type="EnsemblBacteria" id="ADZ08875">
        <property type="protein sequence ID" value="ADZ08875"/>
        <property type="gene ID" value="Metbo_0624"/>
    </dbReference>
    <dbReference type="GeneID" id="10277069"/>
    <dbReference type="KEGG" id="mel:Metbo_0624"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="OrthoDB" id="98644at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="6">
        <location>
            <begin position="116"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="8">
        <location>
            <begin position="248"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="5">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="129"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="30774" checksum="0DBE979724F1EE1C" modified="2011-05-03" version="1">MITGKTNVFGIIGDPVEHSLSPGMHNAAFKELGMDNIYVPFQVKAEHLEDAILGAQSLGVKGFNVTIPHKTEVINYLDYLDIAAGLIGAVNTIEFGKNGAVGHNTDGIGAVMAIEEVTPVKNKKVVILGAGGASRAIAFQLLLSGVDNLVIANRTVEKAGELKTDLVEKLDHEVRVSGLDENLTQELSDTAILINTTPVGMYPNVDEKPVVTAEMMHPDMVVNDIVYNPLKTGLLNEADKAGAKSISGIKMLMYQGVEAFKIWTGIEPPVEIFQRALMNELDLDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC9</accession>
    <name>F0TAC9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type I dehydroquinase</fullName>
            <shortName evidence="1">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroD</name>
        <name evidence="2" type="ordered locus">Metbo_0630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0630"/>
    <dbReference type="EnsemblBacteria" id="ADZ08881">
        <property type="protein sequence ID" value="ADZ08881"/>
        <property type="gene ID" value="Metbo_0630"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0630"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064444_2_1_2"/>
    <dbReference type="OMA" id="ATMAMGE"/>
    <dbReference type="OrthoDB" id="75928at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="3-dehydroquinate binding" evidence="1">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="25108" checksum="C41B371331E4F2E3" modified="2011-05-03" version="1">MKQLFNKTRICVPILEKTYESALESSKSSINAGADILELRIDFIENSDPDAVKNLIQEINFPLIATNRRREENGFFKGSESDRIEILLEAAKVAEIVDIELGTELDNLNKIVKKSKLAIVSYHDFEKTPDKDFLLEIVNKELKLGDIAKFAVMPKTMADTLIVLNVLSEVENTVAISMGDIGSYTRVVAPLFGSPLTFASYENSSAPGQLNIETTRDFINKLLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH6</accession>
    <name>F0TAH6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644279.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0677"/>
    <dbReference type="EnsemblBacteria" id="ADZ08928">
        <property type="protein sequence ID" value="ADZ08928"/>
        <property type="gene ID" value="Metbo_0677"/>
    </dbReference>
    <dbReference type="GeneID" id="10277122"/>
    <dbReference type="KEGG" id="mel:Metbo_0677"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184949_0_0_2"/>
    <dbReference type="OMA" id="TIVPYEM"/>
    <dbReference type="OrthoDB" id="113119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9137" checksum="B7DFAE355BB1A994" modified="2011-05-03" version="1">MRIIEEIIGKEVLDSSAIIIGKVKDVEVNFETKTLESFILGKGGISEGLGISKGETIVPYEMVKKIGDKILLKSAAEDIDEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI0</accession>
    <name>F0TAI0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645403.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1830"/>
    <dbReference type="EnsemblBacteria" id="ADZ10052">
        <property type="protein sequence ID" value="ADZ10052"/>
        <property type="gene ID" value="Metbo_1830"/>
    </dbReference>
    <dbReference type="GeneID" id="10278287"/>
    <dbReference type="KEGG" id="mel:Metbo_1830"/>
    <dbReference type="eggNOG" id="arCOG06500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170805_0_0_2"/>
    <dbReference type="OMA" id="RAYEETH"/>
    <dbReference type="OrthoDB" id="122359at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="13525" checksum="83D3F9AC5CA9CEBA" modified="2011-05-03" version="1">MDSTNLETDIKITEEDVLELLHLFTKVPSLILKGVVARRSNVVKSFEGKILEYKEQLSEDELAKIRVVLEMPTSELQKILHNAYEKTGKKQLKILSEKNAEPFIEKNLDALEKILFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL4</accession>
    <name>F0TAL4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1865</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645437.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1865"/>
    <dbReference type="EnsemblBacteria" id="ADZ10086">
        <property type="protein sequence ID" value="ADZ10086"/>
        <property type="gene ID" value="Metbo_1865"/>
    </dbReference>
    <dbReference type="GeneID" id="24964580"/>
    <dbReference type="KEGG" id="mel:Metbo_1865"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04446">
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
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    <sequence length="481" mass="51753" checksum="9E8530AD89106E16" modified="2011-05-03" version="1">MFKKLFLKKSIEETLSHKPDRSLKRSIGPTGLTLLGLGSIVGAGIFIVTGVASAKFAGPAIIVSFIVSAIACSFTALCYAEFASMLPISGSVYTYTYVTMGEVWAWMIGWVLIFEYLISASAVAVGWSSYVVELLSSVGINLPHMITASIFAGGLINVPAVLIILTLTMILFIGARESTRVNGAIVLINMAIIVIFIIFGLKFINPANYHPFTPYGITGIFQGAAMVFFAYIGFDAVSTAAEETKDPQKNLPKGIIGSLLISSVLYIIVAAVLTGMVPYNLLDTAAPVTFALNYVGLETVASIVGVGAIFGITSVLLTSLFGQTRIFFSMSRDGLLPGLFSKVHPRFRSPVTSILLVGFVASFIAALVPLAAIIELVNIGTLSGFIFLAISIIILRRQNPDLERGFKCPFVPAVPILSIISCVFLITQLSLKTVEYFIISLIIGFSVYMVYGFRNSKIQSTTKSIETMKIESDNVLDTDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM5</accession>
    <name>F0TAM5_METLA</name>
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        </submittedName>
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    <gene>
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    </gene>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ10097.1"/>
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        <property type="gene ID" value="Metbo_1876"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="338" mass="37221" checksum="EBE0529B95B842EC" modified="2011-05-03" version="1">MTENAIEIKNLTKEFGDFKAVDDLSLNVKAGEIFGFLGPNGAGKSTTIRMLCTLAQPTSGSATISGYDLVKDAAKVRENIGLVAEKMIMYDALTAAENLRFFGKLYRIPKVELERRIDELLEMVNMQEWKDTQINKFSTGMKQRINVIRALLPEPKIIFMDEPTLGLDPQTTFSIREIIRDINKKGVTVILTTHAMVEAEALSDRVAIIDHGKIAALDTPENLKNMISDENTTIFSVKITNLTSHLIESIKNLENVTALAQQDDHTLKISATGENALNIIIDSIRAKGGNIFSITNSNESTLEDVFLALTGKEIRDHASEKAAPTSHGHGAKAQARVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM6</accession>
    <name>F0TAM6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1877</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645449.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1877"/>
    <dbReference type="EnsemblBacteria" id="ADZ10098">
        <property type="protein sequence ID" value="ADZ10098"/>
        <property type="gene ID" value="Metbo_1877"/>
    </dbReference>
    <dbReference type="GeneID" id="10278335"/>
    <dbReference type="KEGG" id="mel:Metbo_1877"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_2"/>
    <dbReference type="OMA" id="ANIYRLG"/>
    <dbReference type="OrthoDB" id="71648at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="394" mass="43008" checksum="892F18B63261F1BA" modified="2011-05-03" version="1">MDLIKLFKDSYHIMAKDLLSLRRNKMSLAALFIMPVAFLVMFGFIFPSGGTQTNMPVGMVNLDGGQGSTEFISQLNALNSNNSYLKIQNFTSTDAAKTQINQGKLYGTFIIPPGFSYNMSHGQSANVIMYVDNSNPQMATQMESVTSNTITMMNGQQAVYNVVNMSETTGQTVNPQAVVFPYTPNIATTIPGQNNYFNFLAPGLMIMIVMMTVMTGIPEAISKEKEIGTFDGMLSAPINQISIILGKTAALCVRGFGQCILILVLAVLFFGVTIQGSILLAFFMLLLGIFSFVGIGIVAVSMSEDQASGTMIVNLLMFPMMFLAGIFYPLQQMPWFMQDISKVIPLTYAADAMRKIMLLNAGLGDVITQVLILIAFGVVTMAIAVPLFRRSMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR6</accession>
    <name>F0TAR6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0691"/>
    <dbReference type="EnsemblBacteria" id="ADZ08942">
        <property type="protein sequence ID" value="ADZ08942"/>
        <property type="gene ID" value="Metbo_0691"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0691"/>
    <dbReference type="eggNOG" id="arCOG01123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="34246" checksum="024903997B09BC33" modified="2011-05-03" version="1">MDVTNLKTMYWKDNKLFLIDQTLLPDQIVYHECENYHDVIDAIKTMKVRGAPAIGVSAAFAMALAELAGENMETAAKEIKAARPTAVNLFWAVDRVLNADSALDEAMLMYDEDVETNRKIGKYGASIIDDGDTLLTHCNAGALACVDYGTALGVFRAAKEEGKNINVICDETRPLCQGARLSVFEMQQESIPVRLAVDSAAGHLMQKGMIDKVVIGADRIAKGGVANKIGSLMVALAAKRFDVPFYVAAPKSTFDMENSIFDVEIEERDPEEVIYFGSTRVAPEGTEVENPAFDIVPSDLITGIITEEGILKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB17</accession>
    <name>F0TB17_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645514.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1943"/>
    <dbReference type="EnsemblBacteria" id="ADZ10163">
        <property type="protein sequence ID" value="ADZ10163"/>
        <property type="gene ID" value="Metbo_1943"/>
    </dbReference>
    <dbReference type="GeneID" id="10278403"/>
    <dbReference type="KEGG" id="mel:Metbo_1943"/>
    <dbReference type="eggNOG" id="arCOG02680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110112_1_0_2"/>
    <dbReference type="OMA" id="KHERSFK"/>
    <dbReference type="OrthoDB" id="117224at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="PTHR42195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="23562" checksum="DF066F7F21609E87" modified="2011-05-03" version="1">MNCTQCGSESCEILKSKGKKSKELLLQCNECGNIFRETVEVEKPLKVRVVVSEYESSHKTSVDLYPDEVLEFEGMLDLGGRPAQITSIENNRGGRVMVANVSDIDTIWASYVDIPARIGISVDYGGRILSRKVEVDREFEFTIGDVVKMGTEIFKIRAMKTQDRKMRKGFAKAFVIKRVYGRPDKDLRRYNYDLTSKIVEISNGSVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB31</accession>
    <name>F0TB31_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD synthase</fullName>
            <ecNumber evidence="3">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ribL</name>
        <name evidence="5" type="ordered locus">Metbo_1957</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645528.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1957"/>
    <dbReference type="EnsemblBacteria" id="ADZ10177">
        <property type="protein sequence ID" value="ADZ10177"/>
        <property type="gene ID" value="Metbo_1957"/>
    </dbReference>
    <dbReference type="GeneID" id="10278417"/>
    <dbReference type="KEGG" id="mel:Metbo_1957"/>
    <dbReference type="eggNOG" id="arCOG01222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_2_1_2"/>
    <dbReference type="OMA" id="FAKKHAD"/>
    <dbReference type="OrthoDB" id="79845at2157"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="4">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="5"/>
            <end position="6"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16728" checksum="02FB99EEE6D4CA1F" modified="2011-05-03" version="1">MATGTFDIIHPGHGYYLEESKKIGGEDAHLVVVVARDSTVRSEKRLPVVDENQRLEVVQMLKPVDEAYLGDENDMFKVVEKIKPDIITIGPDQKHNVEKLKNQLKERGIESDVVKVTGYRKAELDSTCKIIKKVQSMEFDPKIFEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB84</accession>
    <name>F0TB84_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rps17</name>
        <name evidence="4" type="ordered locus">Metbo_0784</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644386.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0784"/>
    <dbReference type="EnsemblBacteria" id="ADZ09035">
        <property type="protein sequence ID" value="ADZ09035"/>
        <property type="gene ID" value="Metbo_0784"/>
    </dbReference>
    <dbReference type="GeneID" id="10277232"/>
    <dbReference type="KEGG" id="mel:Metbo_0784"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OMA" id="KKYERYE"/>
    <dbReference type="OrthoDB" id="108145at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_S17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_S17_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_S17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="12149" checksum="1651774D489469D0" modified="2011-05-03" version="1">MVGIDVKEPKNKCDDPNCPFHGTLPVRGQILEGIVTSEKAERTITVERSFYKFITKYERYEKRKSKIKAHKPDCLDVKIGDSVKIAECRPLSKTKHFVLVEVKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB92</accession>
    <name>F0TB92_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L32e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0792</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644394.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0792"/>
    <dbReference type="EnsemblBacteria" id="ADZ09043">
        <property type="protein sequence ID" value="ADZ09043"/>
        <property type="gene ID" value="Metbo_0792"/>
    </dbReference>
    <dbReference type="GeneID" id="10277240"/>
    <dbReference type="KEGG" id="mel:Metbo_0792"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_3_1_2"/>
    <dbReference type="OMA" id="HPSGYEE"/>
    <dbReference type="OrthoDB" id="110802at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_L32e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_L32e_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="PTHR23413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="SSF52042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="114" mass="13293" checksum="C8FB461A70C39A27" modified="2011-05-03" version="1">MQKPKKPNFKRQEWFRYKKLGDKYRKPKGKTSKRRRYEARKPAMPSIGYRTPKNIRGLHPSGYRDVLVSNLKDLENLDPLTQAGRISATVGARKKANMLERAKELKIKILNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD2</accession>
    <name>F0TBD2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09083">
        <property type="protein sequence ID" value="ADZ09083"/>
        <property type="gene ID" value="Metbo_0832"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0832"/>
    <dbReference type="HOGENOM" id="CLU_2010116_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="14484" checksum="2741F086397DD009" modified="2011-05-03" version="1">MIIGDIMLETKTKEILKNNTFFFQFVDEDHKELSPDQSEALRKFKTVDGSKYKMALRSNAMPKSVQIDLKIIYPPDFYHPNIYELLDFNETMFQSFYNDQMNFSNANEYTKVSESNSTKIRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK0</accession>
    <name>F0TBK0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase assembly chaperone hypC/hupF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645620.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2050"/>
    <dbReference type="EnsemblBacteria" id="ADZ10269">
        <property type="protein sequence ID" value="ADZ10269"/>
        <property type="gene ID" value="Metbo_2050"/>
    </dbReference>
    <dbReference type="GeneID" id="10278512"/>
    <dbReference type="KEGG" id="mel:Metbo_2050"/>
    <dbReference type="eggNOG" id="arCOG04427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159381_2_2_2"/>
    <dbReference type="OMA" id="MCLAIPC"/>
    <dbReference type="OrthoDB" id="119258at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="82" mass="8937" checksum="17128ADF9D258F68" modified="2011-05-03" version="1">MCIAAPARIVDITDNVATVDFGGVKQSAKLDLVGDLEIGTHVLVHSGYAIEVLSEQEAKESLEAWDLLLEALDEEDKERNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR0</accession>
    <name>F0TBR0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644488.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0888"/>
    <dbReference type="EnsemblBacteria" id="ADZ09137">
        <property type="protein sequence ID" value="ADZ09137"/>
        <property type="gene ID" value="Metbo_0888"/>
    </dbReference>
    <dbReference type="GeneID" id="10277337"/>
    <dbReference type="KEGG" id="mel:Metbo_0888"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_1_2"/>
    <dbReference type="OMA" id="AYHIPRR"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="15"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="43010" checksum="3700DDE015F0777C" modified="2011-05-03" version="1">MKVLLVNYLETTFPGGISKTVNELAQNLSKNHEVTVLQPNPSNLRAEETNNGFKIIRVSSALDNTKYFYGLNFEIYKYLKNHYKDLNPDIVHVHGYHHLMPIESIHIIKRMDPNVPIIFSPYLDVARGRLAGKYLWNLYNIFARPVFSKSVSITSCSNFESANIQNIGVEREKITVIPLGVDEINLKKDRKDKNSKKTINLLYTGHIIPRKGVDYILKSLHSLVHKKGADNVVLTVVGDGPEKNKLIKMADELEIADHINWMSFLSREELIDLIRESDIYMLLSRSEAYGIAVAEALAMGTPCVISNSTALTEFSNEIGCYAVDYPPVPDDVADTVLKIYAEDRGVGPLSDRIRTWNEVSKDYENLYQSVVSANKIDKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR1</accession>
    <name>F0TBR1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644489.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0889"/>
    <dbReference type="EnsemblBacteria" id="ADZ09138">
        <property type="protein sequence ID" value="ADZ09138"/>
        <property type="gene ID" value="Metbo_0889"/>
    </dbReference>
    <dbReference type="GeneID" id="10277338"/>
    <dbReference type="KEGG" id="mel:Metbo_0889"/>
    <dbReference type="eggNOG" id="arCOG00563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587455_0_0_2"/>
    <dbReference type="OMA" id="PPLFHIM"/>
    <dbReference type="OrthoDB" id="27401at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="525" mass="59008" checksum="9FF5EC1E66E0A2AD" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT4</accession>
    <name>F0TBT4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0912</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0912"/>
    <dbReference type="EnsemblBacteria" id="ADZ09161">
        <property type="protein sequence ID" value="ADZ09161"/>
        <property type="gene ID" value="Metbo_0912"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0912"/>
    <dbReference type="eggNOG" id="arCOG00252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_2"/>
    <dbReference type="OMA" id="GHCLPID"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="9">
        <location>
            <begin position="322"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="46627" checksum="BE37C6D0D0F46609" modified="2011-05-03" version="1">MNNQTRIALYGLGYIGLPTASLLAKNGYKVVGVDINPQTVEKINSGISPIMEPGLETIISDMVANKMLSATTNGVEAAVNSNIMIVVVPTPVDNNNCSDLTAVISACETISKGLKKDDLVIIESTSPPYTCENVVIPILEKSGLKSGKDFGVAYTPERALPNNTIYEMTHNARIIGGINPESSAQAAEIYGSITEGKVVTVKDTVTAEMVKLMENTYRDTNIALANELAMVCESINIDVIEAIEASNYHPRVNIHTPGPGVGGHCLSIDPYFIVEIAKENGVPARLIQTAREINEHMPNHVASLVEKSLKNKGKSISSSTVGVLGVAYKGNVADYRETPSKNLIDTLNGLGAKVLANDPYVDPEIIRRMEAEPVDLKEALNCDCVVLMTDHDDYRSITPEMIKKSILICNRPILDQDLFKSSGIEFKGVGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ0</accession>
    <name>F0TBZ0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Succinate dehydrogenase or fumarate reductase, flavoprotein subunit</fullName>
            <ecNumber evidence="11">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2113</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2113"/>
    <dbReference type="EnsemblBacteria" id="ADZ10332">
        <property type="protein sequence ID" value="ADZ10332"/>
        <property type="gene ID" value="Metbo_2113"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2113"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_2"/>
    <dbReference type="OMA" id="DPIPIQP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="9">
        <location>
            <begin position="35"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="10">
        <location>
            <begin position="473"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="596" mass="65819" checksum="0059C7AD6D4B1888" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC02</accession>
    <name>F0TC02_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="6">1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2125"/>
    <dbReference type="EnsemblBacteria" id="ADZ10344">
        <property type="protein sequence ID" value="ADZ10344"/>
        <property type="gene ID" value="Metbo_2125"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2125"/>
    <dbReference type="eggNOG" id="arCOG01484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="OMA" id="CECGQEV"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="10">
        <location>
            <begin position="9"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="24963" checksum="C9FE1B72C8876430" modified="2011-05-03" version="1">MSPKNYEKPYTILNSAMTLDGKIATKTGSSEISGQEDLVRVHELRMEVDAIMVGINTVLADDPRLTVHKIPSSKSDNPLRIVVDSRARTPLNFRILNSDAKTLLAVTQAADPVKVNKLKQLVDVVVCGEKHVDLKNLMKHLYELGIKTLMLEGGSTLNYSMLSEGLVDEVRVCIAPMVAGGKYAKTLADGDGFDEMKDSVKLEFKKSYTLGDDLIVEYKVLPRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC20</accession>
    <name>F0TC20_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644522.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0922"/>
    <dbReference type="EnsemblBacteria" id="ADZ09171">
        <property type="protein sequence ID" value="ADZ09171"/>
        <property type="gene ID" value="Metbo_0922"/>
    </dbReference>
    <dbReference type="GeneID" id="10277371"/>
    <dbReference type="KEGG" id="mel:Metbo_0922"/>
    <dbReference type="HOGENOM" id="CLU_2056067_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="12487" checksum="8C0E31B322F1D5E5" modified="2011-05-03" version="1">MDKDYKLTAVLIPIILILSLTLYFFPESSGMVTNFTAPVVNQTNNTTINQTASNSTAVESATSTQNTQTSQSTDTSYNSNSGSDSGSGDTPTEPTEPTDPTEPTDPTEPTDPTDTGNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC45</accession>
    <name>F0TC45_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644547.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09196">
        <property type="protein sequence ID" value="ADZ09196"/>
        <property type="gene ID" value="Metbo_0949"/>
    </dbReference>
    <dbReference type="GeneID" id="10277398"/>
    <dbReference type="KEGG" id="mel:Metbo_0949"/>
    <dbReference type="eggNOG" id="arCOG06690">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067844_0_0_2"/>
    <dbReference type="OMA" id="TWFYIAV"/>
    <dbReference type="OrthoDB" id="111439at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13912" checksum="8DB45A65BE4C5474" modified="2011-05-03" version="1">MEMTWFYIAVFLAISDEIHSRIMWGVFADFYILLAGVIKESVASNIRLWIVHESMEAFFHFVILSLVFLSLEIGLLAALIHMLVDIYHEISGIELNPLGHRCLHFVIESIFFIVIFAAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC49</accession>
    <name>F0TC49_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Na+/Ca+ antiporter, CaCA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0953</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644551.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0953"/>
    <dbReference type="EnsemblBacteria" id="ADZ09200">
        <property type="protein sequence ID" value="ADZ09200"/>
        <property type="gene ID" value="Metbo_0953"/>
    </dbReference>
    <dbReference type="GeneID" id="10277402"/>
    <dbReference type="KEGG" id="mel:Metbo_0953"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007948_0_3_2"/>
    <dbReference type="OMA" id="TRDYGVM"/>
    <dbReference type="OrthoDB" id="45269at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00367">
        <property type="entry name" value="TIGR00367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="7">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="7">
        <location>
            <begin position="195"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="339" mass="36048" checksum="B1CBFE69D620E88D" modified="2011-05-03" version="1">MIDLIIFIIILIVALIIVIKSADLFVDSLVEVGESLGISQIILGVTASAIGTSLPEFGSAMIAVFSGNPDIGVGVVIGSNIWNIAGILGISSLAAGYIFVKKEELKRDGLMTLLTAAILLIFMVLFNDITRIVGVVLVVVYIIYLYILIKSQHKKSNSEDSEEESSESKKPEISGSGNDLVEEVMVSGRSRNKNILISIAGLAGLILGSRLLVYSGVEIGELTGVPQIIMGLFALAIGTSMPELVVTVRSAMKKMHSLSIGTVLGSNIFNILIGIGIPSLFMTIPVDKLAVSFDAPVMIGVTALLIILSYRKSKLSRIGGAVLISAYVAYIVTRIYLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD2</accession>
    <name>F0TCD2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rrp42</name>
        <name evidence="5" type="ordered locus">Metbo_2184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2184"/>
    <dbReference type="EnsemblBacteria" id="ADZ10399">
        <property type="protein sequence ID" value="ADZ10399"/>
        <property type="gene ID" value="Metbo_2184"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2184"/>
    <dbReference type="eggNOG" id="arCOG01574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_2"/>
    <dbReference type="OMA" id="PESVFEM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_PH" evidence="3">
        <location>
            <begin position="30"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="RNase_PH_C" evidence="4">
        <location>
            <begin position="194"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="28637" checksum="2E90C9CF624114C2" modified="2011-05-03" version="1">MVHIVPEIIKESVTNLVKNGERTDGRAFDEYREISLEAGVIEKAEGSARVKIGNTQIIIGAKPQIGTPFGDTPNVGVLMTNTELLPMAAPNFESGPPDENSVELSRVTDRCIREGEVVDLEKLCIVPGEKVWMIFLDLHVVDYDGNLMDAAVLGSVAALMNTRLPEAKIIDDEVVLDYDNLIPLPINDTALMSTFAKIGEELIIDPSLEEEEVMGARLSIGITKDGNICAMQKGGEKTLTQEEVLKAVSIAKIKTAELRKLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH9</accession>
    <name>F0TCH9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644607.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1009"/>
    <dbReference type="EnsemblBacteria" id="ADZ09256">
        <property type="protein sequence ID" value="ADZ09256"/>
        <property type="gene ID" value="Metbo_1009"/>
    </dbReference>
    <dbReference type="GeneID" id="10277458"/>
    <dbReference type="KEGG" id="mel:Metbo_1009"/>
    <dbReference type="eggNOG" id="arCOG04444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136790_2_1_2"/>
    <dbReference type="OMA" id="INIEYLY"/>
    <dbReference type="OrthoDB" id="89042at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045739">
        <property type="entry name" value="ACT_dom_pair"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19571">
        <property type="entry name" value="ACT_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15780" checksum="1F4704F42298A2E4" modified="2011-05-03" version="1">MKLKQISVFLENRKGRLWKALNILSNAKINIRALSIADTSEFGILRMIVSDTELAKQILEESNFVVKVNEVIAVGVSDNPGGLEGVLEIFNEMDVNVEYIYAFVEKNGEKAVVVIRTEDIDAGVNALKNGGITILSEEEIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ4</accession>
    <name>F0TCJ4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1024"/>
    <dbReference type="EnsemblBacteria" id="ADZ09271">
        <property type="protein sequence ID" value="ADZ09271"/>
        <property type="gene ID" value="Metbo_1024"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1024"/>
    <dbReference type="eggNOG" id="arCOG04964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352083_0_0_2"/>
    <dbReference type="OMA" id="LMQYGEK"/>
    <dbReference type="OrthoDB" id="88686at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="21070" checksum="B496F53B1C81B526" modified="2011-05-03" version="1">MFVDDAVNIVKCLKGVLGVQLLSKDAKGALLKIESFHEDDFIPVINEGLNKCLKREFCLVMFKTAEFRIPPEPTVILKTDTGKILGRELISNEDKENYSNRNDVIFLSNNFILFKPEKSKNRDVIEKQQFILPPIPFPELDELGEITDIISGTPSTMGDEYIKNIYGYPEDPKIATVLIGFSKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP7</accession>
    <name>F0TCP7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Precorrin-6x reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2223</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645788.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2223"/>
    <dbReference type="EnsemblBacteria" id="ADZ10437">
        <property type="protein sequence ID" value="ADZ10437"/>
        <property type="gene ID" value="Metbo_2223"/>
    </dbReference>
    <dbReference type="GeneID" id="10278689"/>
    <dbReference type="KEGG" id="mel:Metbo_2223"/>
    <dbReference type="eggNOG" id="arCOG04852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068627_0_0_2"/>
    <dbReference type="OMA" id="THPFAIN"/>
    <dbReference type="OrthoDB" id="94583at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="PTHR36925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00715">
        <property type="entry name" value="precor6x_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="28837" checksum="18686E3870167BF0" modified="2011-05-03" version="1">MNVMVMSGTSDAVKIINYLHAIDDTVITATTTTSYGAKLASDAGADEVKVGGLGVEAMVELIQQKEIDLLLDATHPFASQATETALDAANSAKIEYLRFERPATQHHTIKGIHVVKDFKEASKTASELVSKTKNGRIMHLAGVSTLPQVLEKISAHSIIVRVLPAVYSINKCLELGIPSENIVAMQGTFSSDFNRALMEEFNVNVVITKESGESGGTLSKLEAASQLEIPVVMVKRPEIKQLKKSKVYSDIPELINEVKRFKSNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV4</accession>
    <name>F0TCV4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_2280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645845.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2280"/>
    <dbReference type="EnsemblBacteria" id="ADZ10494">
        <property type="protein sequence ID" value="ADZ10494"/>
        <property type="gene ID" value="Metbo_2280"/>
    </dbReference>
    <dbReference type="GeneID" id="10278746"/>
    <dbReference type="KEGG" id="mel:Metbo_2280"/>
    <dbReference type="eggNOG" id="arCOG04858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="OMA" id="GHRNPGE"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="30293" checksum="2BFBFE4540995F76" modified="2011-05-03" version="1">MSYEVQYTPGNTKIAENRRKHMDPDFELKKIREIADEDIVKILGHRNPGEGYKSVHPPLEEMDFEEDLMKEMVEPIEGAKEGNRTRYVQFTDSMYNAPAQPYDRARTYMWRFRGVDTGTLSGRQVIEIRERDLEKISKTLVETELFDPAKTGIRGATVHGHSLRLDENGLMFDALQRYVFDEKTGSVSYIKDQVGRPLDEPVDMGAPLDEENLKEITTIYRSDNISMRDDPEAIETVEAIHAARTDGGFGLEVFKDDLKRKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S1</accession>
    <name>F0T5S1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644665.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1068"/>
    <dbReference type="EnsemblBacteria" id="ADZ09314">
        <property type="protein sequence ID" value="ADZ09314"/>
        <property type="gene ID" value="Metbo_1068"/>
    </dbReference>
    <dbReference type="GeneID" id="10277517"/>
    <dbReference type="KEGG" id="mel:Metbo_1068"/>
    <dbReference type="eggNOG" id="arCOG01522">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026719_1_0_2"/>
    <dbReference type="OMA" id="YHEEVSS"/>
    <dbReference type="OrthoDB" id="68194at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="537" mass="61982" checksum="4D04E3309D87D6F8" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S3</accession>
    <name>F0T5S3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1801 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09316">
        <property type="protein sequence ID" value="ADZ09316"/>
        <property type="gene ID" value="Metbo_1070"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1070"/>
    <dbReference type="eggNOG" id="arCOG14002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133756_0_0_2"/>
    <dbReference type="OrthoDB" id="114435at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014922">
        <property type="entry name" value="DUF1801"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08818">
        <property type="entry name" value="DUF1801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1801" evidence="1">
        <location>
            <begin position="42"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14953" checksum="FB0DBDFB989E322B" modified="2011-05-03" version="1">MNKSTENIGSKKKPVKRGVKMDGETAVLEKINDMAEPYQTMGKKIHKLIKTTAPELSPRTWYGMPAYSKEGKVLCFFRSGDKFKERYMTLGFNDSANLDEGNMWPVAFALTELTLSEEKRIVDLIKKAANTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T4</accession>
    <name>F0T5T4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Triphosphoribosyl-dephospho-CoA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644678.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1082"/>
    <dbReference type="EnsemblBacteria" id="ADZ09327">
        <property type="protein sequence ID" value="ADZ09327"/>
        <property type="gene ID" value="Metbo_1082"/>
    </dbReference>
    <dbReference type="GeneID" id="10277531"/>
    <dbReference type="KEGG" id="mel:Metbo_1082"/>
    <dbReference type="eggNOG" id="arCOG04238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="OMA" id="GNTHFGA"/>
    <dbReference type="OrthoDB" id="62406at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="305" mass="33495" checksum="225E6DBD30AE7CA7" modified="2011-05-03" version="1">MDPDFIAKTAQIASVLEVSGYPKPGNVHRTQDFSDMVFEDFLISGVVIGNIMKKAAKLGQIHGSNSREMSQIEVGKLIKEAVVETDKWIKNNTNLGIIMLLTPISAAAGMSDEIDELQYSVDEILRATTPQDAVDLYEAINIAEAGGMGVQDDLDVSNDNAKAELIEKNINMFDVLDLSSQWDMLANELTRTMPITFELGYPTFKEVRKDHGINKATVQTFLTILSIYPDTLIARKYGLETAQEVSVDAGLIIKEGGALTDNGMDMLMEFDNDLVKKKLNPGTTADLTASSVMVALLDEYKTNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U7</accession>
    <name>F0T5U7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Copper-translocating P-type ATPase</fullName>
            <ecNumber evidence="6">3.6.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.4" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644691.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1095"/>
    <dbReference type="EnsemblBacteria" id="ADZ09340">
        <property type="protein sequence ID" value="ADZ09340"/>
        <property type="gene ID" value="Metbo_1095"/>
    </dbReference>
    <dbReference type="GeneID" id="10277544"/>
    <dbReference type="KEGG" id="mel:Metbo_1095"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_11_2_2"/>
    <dbReference type="OMA" id="ACGMNMV"/>
    <dbReference type="OrthoDB" id="21472at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="658"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="685" mass="74509" checksum="305427F72C67DDBE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W3</accession>
    <name>F0T5W3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644707.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1111"/>
    <dbReference type="EnsemblBacteria" id="ADZ09356">
        <property type="protein sequence ID" value="ADZ09356"/>
        <property type="gene ID" value="Metbo_1111"/>
    </dbReference>
    <dbReference type="GeneID" id="10277561"/>
    <dbReference type="KEGG" id="mel:Metbo_1111"/>
    <dbReference type="eggNOG" id="arCOG14845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1458205_0_0_2"/>
    <dbReference type="OrthoDB" id="138263at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="21506" checksum="7928495EB7115648" modified="2011-05-03" version="1">MQKKGFLGFFKYIFGGFVILYWMFITGIPSILGYVYIPNGITLDRPSLAILTIIIFTLIFLCSWLIYQGWQVYHTSFAQNDLPKIVDFLRSDNYGEEFVFLVENESNINSGKIAELRRYSHGVEVSFALIEFMEKNSKGQFQAKHIWFSPGHLRELKLNCFSVSEITVDLNVNINTLNKYMEELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X6</accession>
    <name>F0T5X6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09369">
        <property type="protein sequence ID" value="ADZ09369"/>
        <property type="gene ID" value="Metbo_1124"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1124"/>
    <dbReference type="HOGENOM" id="CLU_1745567_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17282" checksum="D04BF7FF8AC19E3F" modified="2011-05-03" version="1">MFTGNLFKTFKLIYSERVIMEISAIFVLIMSILVLIFSILVINHYRLHPEKDGTILSISLVTLGIILVIGAITDLTSLLILLYLIIFLLYYKYTLNKNPEIRKNREKGKEILKKHPTYKILRISQYIMLACAIFLSLIISIIVIFNIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T601</accession>
    <name>F0T601_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2294"/>
    <dbReference type="EnsemblBacteria" id="ADZ10508">
        <property type="protein sequence ID" value="ADZ10508"/>
        <property type="gene ID" value="Metbo_2294"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2294"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="17317" checksum="19BC1CE8A4F7BA4F" modified="2011-05-03" version="1">MKMGSKKYTSFEGSVVNGNVSIDEKSSVWYNAVIRGDIEPIKIGKCSNVQDNCVLHSSKGYPLTIGNKVSVGHAAVLHGCDIEDNCLIGMNATVLNGALIRKNSIVGAGALVTENHEFPENSLILGVPAKAVRTLKNEEIENITNNAIRYSKMALKSDLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T616</accession>
    <name>F0T616_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645874.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10523">
        <property type="protein sequence ID" value="ADZ10523"/>
        <property type="gene ID" value="Metbo_2309"/>
    </dbReference>
    <dbReference type="GeneID" id="10278775"/>
    <dbReference type="KEGG" id="mel:Metbo_2309"/>
    <dbReference type="eggNOG" id="arCOG14543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987639_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="14807" checksum="A50B4F8484AEA43C" modified="2011-05-03" version="1">MIFDRVFLDFDAENEEARKIKNKLIKLRCYGLNYKKSFQNKLQEKLQKLVINEKISKPAVDKAKDFVQNFKETFGKPPILFFSGFKGCHAYLFFKPVEFQNINLAISWFAKNTKKAYNYSTLIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T644</accession>
    <name>F0T644_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridylate kinase</fullName>
            <shortName evidence="6">UK</shortName>
            <ecNumber evidence="6">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Uridine monophosphate kinase</fullName>
            <shortName evidence="6">UMP kinase</shortName>
            <shortName evidence="6">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrH</name>
        <name evidence="8" type="ordered locus">Metbo_2338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645902.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2338"/>
    <dbReference type="EnsemblBacteria" id="ADZ10551">
        <property type="protein sequence ID" value="ADZ10551"/>
        <property type="gene ID" value="Metbo_2338"/>
    </dbReference>
    <dbReference type="GeneID" id="10278804"/>
    <dbReference type="KEGG" id="mel:Metbo_2338"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OMA" id="MGGTHPG"/>
    <dbReference type="OrthoDB" id="48934at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="24471" checksum="26BA31C5CB9B4A30" modified="2011-05-03" version="1">MKIVVTIGGSILIKDHDYKKFRDYANVLKKLNQDNEVFVVVGGGKTARDYIDIARSLEVSEAMCDDVGIEVTRLNAKLLIAALGKDAYPDVPHNFREALTYANSGNIIVMGGTEPAHSTDAVGSILAEFVGSDLLINATSVDGLYNKDPNKYPDAVMFKEVKPSKMMELMSHNDVKAGTYEFFDMTAIQIIKRSSIKTVIVNGEDPKNILIANEGNIGTTIIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T648</accession>
    <name>F0T648_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="4">PBG</shortName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="4">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hemC</name>
        <name evidence="7" type="ordered locus">Metbo_2342</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="4">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645906.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2342"/>
    <dbReference type="EnsemblBacteria" id="ADZ10555">
        <property type="protein sequence ID" value="ADZ10555"/>
        <property type="gene ID" value="Metbo_2342"/>
    </dbReference>
    <dbReference type="GeneID" id="10278808"/>
    <dbReference type="KEGG" id="mel:Metbo_2342"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="95523at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="5">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="6">
        <location>
            <begin position="217"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="4">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="290" mass="31875" checksum="DD1B866EC53DF5E0" modified="2011-05-03" version="1">MKVGTRGSSLATYQTKTIINELSIVTDEKIDVEIIKSTGDKIQNSQLYNIDAKGIFTRELDDAVLDGTVDFAVHSLKDLPTELEGDLEIVAVPERESPNEVLVSKLGWDELPKGATVGTSSIRREAFCNHHDKEFNIKPIRGNIDTRIKKVFSGEYDATIMAEAGLNRLGLTEYIKQRFSIEYFTPAAGQGAIAVVARSDSDVKDDLQKINHLKSFQEVTAEKRVLLEMGVGCQSPLGVSARINENKLKLCAVLLTQEGKIVSRVMVEGSADKASNLGLKAAKLMMEDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I7</accession>
    <name>F0T6I7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase</fullName>
            <ecNumber evidence="3">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645972.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2408"/>
    <dbReference type="EnsemblBacteria" id="ADZ10621">
        <property type="protein sequence ID" value="ADZ10621"/>
        <property type="gene ID" value="Metbo_2408"/>
    </dbReference>
    <dbReference type="GeneID" id="10278880"/>
    <dbReference type="KEGG" id="mel:Metbo_2408"/>
    <dbReference type="eggNOG" id="arCOG01607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_1_2"/>
    <dbReference type="OMA" id="DFIQGDE"/>
    <dbReference type="OrthoDB" id="34838at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="17"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="274"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="375" mass="41475" checksum="7ADE06C3F2C7EA94" modified="2011-05-03" version="1">MKIEQYFIQGNEACARGAIHAGCRFFAGYPITPSTEIAEDMAVFLPKEGGTFIQMEDEIAALGAVIGAVWGGVKGMTATSGPGFSLMQEHIGYATMTETPLVIVNMQRGSPSTGQPTMASQSDMMQARWGSHGDYEIIALSPSSVQECFDFTVRAFNLAEEYRVPVMVMADEIVGHMREKITIPETTNITPRKMPDESPETFLPYKADPEGTSKMPAFGDGYKLQITGLTHDERGYPDASNPKTHSKLVNRLVNKIIKNTDKLASYKSFNTEDADVIIVSYGAPSRSVTTAVKNAREEGIKAGYIKLETVWPFPEDILRKVAKNAQRVIVVEMNLGQIYHEVQRVLKDHDVELIPKIGGEMHKPDEILKIIEKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M0</accession>
    <name>F0T6M0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2441</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2441"/>
    <dbReference type="EnsemblBacteria" id="ADZ10654">
        <property type="protein sequence ID" value="ADZ10654"/>
        <property type="gene ID" value="Metbo_2441"/>
    </dbReference>
    <dbReference type="GeneID" id="10278913"/>
    <dbReference type="KEGG" id="mel:Metbo_2441"/>
    <dbReference type="eggNOG" id="arCOG01044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_2_2"/>
    <dbReference type="OMA" id="THVVVPY"/>
    <dbReference type="OrthoDB" id="94240at2157"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:siroheme biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
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        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="cysG_Nterm"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="23353" checksum="B86E79C13961E952" modified="2011-05-03" version="1">MGWTPLYLNMADKKVLIVGSGEVGSRRCERFLDAGAKVVLIGDHVNDDLVQLGAIVKSIDELGLWIDWADIVVAASGDHELNNRVGEMSGDKLINRADNPNDGNLIVPSSFFIGDVQICIFTGGKSPLMSKELRKKIQKVIKPEDIYQLELQYYARNILKQNIDDQKIRREYLYKILEDLKIKELLNKGDLESAKKHASTIIDTIDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q4</accession>
    <name>F0T6Q4_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ08287">
        <property type="protein sequence ID" value="ADZ08287"/>
        <property type="gene ID" value="Metbo_0034"/>
    </dbReference>
    <dbReference type="GeneID" id="10276456"/>
    <dbReference type="KEGG" id="mel:Metbo_0034"/>
    <dbReference type="eggNOG" id="arCOG13989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881076_0_0_2"/>
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    <dbReference type="OrthoDB" id="130801at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13157" checksum="EA43BFC6F927ECA6" modified="2011-05-03" version="1">MNWKLLGLGACVNAILTGIITIIYLPLAFIGPFTGGFLVSYLSKGYEDYNGIDWKDGAVVGAISGLIGGLILALLFIWLGTWNAALESLTGNNLILISYVILQVTVLLSFILGLIGGVVGVALKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V7</accession>
    <name>F0T6V7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1235"/>
    <dbReference type="EnsemblBacteria" id="ADZ09477">
        <property type="protein sequence ID" value="ADZ09477"/>
        <property type="gene ID" value="Metbo_1235"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1235"/>
    <dbReference type="eggNOG" id="arCOG02358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OMA" id="NDILWEF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="HisK_dim/P"/>
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    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS-assoc_C"/>
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    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
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            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="228"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="282"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="437"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="557"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="769" mass="87891" checksum="1A7F4ACA52133706" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y3</accession>
    <name>F0T6Y3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase M24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644854.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1261"/>
    <dbReference type="EnsemblBacteria" id="ADZ09503">
        <property type="protein sequence ID" value="ADZ09503"/>
        <property type="gene ID" value="Metbo_1261"/>
    </dbReference>
    <dbReference type="GeneID" id="10277712"/>
    <dbReference type="KEGG" id="mel:Metbo_1261"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_10_0_2"/>
    <dbReference type="OMA" id="RMRAFNG"/>
    <dbReference type="OrthoDB" id="28553at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="2">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="1">
        <location>
            <begin position="143"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="398" mass="44774" checksum="039765D189E67FA5" modified="2011-05-03" version="1">MITKTPLKELESRMKNFRSFMDEQCPGWEFAVLFSKINLYYFTGTMQDGMLIIEKNGSTFWIRKSFERAKIESLFEDLKPMNSYRDAAAMYADLPETVYIETEVVPLAMYSRFQRHFPFKNFESLDHVVARLRSIKSHYELSLMRRAGEIHRHVLEDLVPEILVDGMSETEFASDLFKLMMDEGHHGVSRFGMFDTEMVLGQLGFGESSIHPSYFNGPGGNSGISPAVPLIGSRDRKLQGGDLVFVDVGCGVEGYNTDKTMTYMYQAPLKKEAIEAHEICVEIQNNIASMLKPGAVPSEIYRTVIDDLDPEFLENFMGYENRTVKFLGHGIGLLIDETPVIAKGFNEPIEEGMVFAVEPKKGIKGVGMVGIENTFIVTKKGGDCITGKNPGLIPVDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T701</accession>
    <name>F0T701_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644872.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1279"/>
    <dbReference type="EnsemblBacteria" id="ADZ09521">
        <property type="protein sequence ID" value="ADZ09521"/>
        <property type="gene ID" value="Metbo_1279"/>
    </dbReference>
    <dbReference type="GeneID" id="10277730"/>
    <dbReference type="KEGG" id="mel:Metbo_1279"/>
    <dbReference type="eggNOG" id="arCOG04834">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366257_0_0_2"/>
    <dbReference type="OMA" id="MNEVPFM"/>
    <dbReference type="OrthoDB" id="110204at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019211">
        <property type="entry name" value="EhaL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09877">
        <property type="entry name" value="DUF2104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="11659" checksum="2451D5D48C1EB550" modified="2011-05-03" version="1">MNDISYLIYILSFIIGSAMGLVLSYKKYTAPFVTKNIDMVALILAIVGWVLAINSQLISAWVSPVITITVGIFLIAIVMGMRPGYGRYETVIGFAISALIWVGTVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T731</accession>
    <name>F0T731_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate/ketoisovalerate oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2452</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646016.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2452"/>
    <dbReference type="EnsemblBacteria" id="ADZ10665">
        <property type="protein sequence ID" value="ADZ10665"/>
        <property type="gene ID" value="Metbo_2452"/>
    </dbReference>
    <dbReference type="GeneID" id="10278924"/>
    <dbReference type="KEGG" id="mel:Metbo_2452"/>
    <dbReference type="eggNOG" id="arCOG01600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042621_0_0_2"/>
    <dbReference type="OMA" id="RGGTSNC"/>
    <dbReference type="OrthoDB" id="24786at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="3">
        <location>
            <begin position="68"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="310"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="481" mass="52370" checksum="3BDABEDD846DF930" modified="2011-05-03" version="1">MSNELKDEKILGKPETILSEFPRKGGSAPTATHYCSGCGHGILHKLIGEAVDDLKIQDRMVMTSPVGCAVFAYYYFDCGHVQVAHGRAPAVGTGLSRAEEDAVVILYQGDGDLASIGLNETIQAANRGEKMAVFFINNTVYGMTGGQMAPTTLIGEVTVTCQDGRDPRYTGYPLHMSELLNNLKAPVFIERVSLSDPSHIRKARRAVRKALEIQRDGKGYAFVELLSPCPTNLRQDAEGAEKFLNEQMEVEFPLANFRDRTAEVEPLCRESSDFSRESLDKIFDVGEESSLGAIDDPEFETKVVKISGFGGQGVLSMGLILAEAACKARRHVSWYPSYGPEQRGGTSNCTVIVSGEIIGSPVVYRSDILVALNRPSLEEFAERVKQGGTILYDEEIGDFKPPEGVKAIGVPALKIAKENGVGKAANTAMLGVLMEVSDDMSVDDFKSAISQTFAKKPHLIDLNLEVLYAAAEWARENFTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T754</accession>
    <name>F0T754_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PKD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2475"/>
    <dbReference type="EnsemblBacteria" id="ADZ10688">
        <property type="protein sequence ID" value="ADZ10688"/>
        <property type="gene ID" value="Metbo_2475"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2475"/>
    <dbReference type="eggNOG" id="arCOG02508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02516">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260893_0_0_2"/>
    <dbReference type="OMA" id="NVKDYNW"/>
    <dbReference type="OrthoDB" id="109at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="361"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="616"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="699"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="782"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="863"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1208" mass="129833" checksum="910ADF427210CA22" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T759</accession>
    <name>F0T759_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646044.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2480"/>
    <dbReference type="EnsemblBacteria" id="ADZ10693">
        <property type="protein sequence ID" value="ADZ10693"/>
        <property type="gene ID" value="Metbo_2480"/>
    </dbReference>
    <dbReference type="GeneID" id="10278954"/>
    <dbReference type="KEGG" id="mel:Metbo_2480"/>
    <dbReference type="eggNOG" id="arCOG03302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_13_1_2"/>
    <dbReference type="OMA" id="CKMSSNY"/>
    <dbReference type="OrthoDB" id="90656at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="53"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="357" mass="38857" checksum="9B8F25DB0BC470F6" modified="2011-05-03" version="1">MKDQKIIIISLIAILAVVGLIWTYVNLQDTSPNNGQITDMAGRTVSVPSEINKTYSTAGSVTILLYMLAPDTMIGWNSQNGTNNYMPDKYKTLPVLGGGQGQGEGTANYEGVISKGPDIVFVGHGGDNDTINKIQQKFGEIPALDIEGDNNLTNIIPSIKFMGTALGNQNQANDLINFYTTVLDQVNSTVSTIPDSEKKKVYYARSSDGLTTFAAGSPQTQLIAISGGKSVVQAPVASGGMAVSMEMVISWNPDVIITSSSEFYNSVYNNQLWQNITAVKNKQVYLTPQDPFNWFESPPGANTIIGIPWTAKVLYPDKFQDLDLNNLTQTFYSKFYHYNLTEEQVSNILSSSGLGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T799</accession>
    <name>F0T799_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643679.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0075"/>
    <dbReference type="EnsemblBacteria" id="ADZ08328">
        <property type="protein sequence ID" value="ADZ08328"/>
        <property type="gene ID" value="Metbo_0075"/>
    </dbReference>
    <dbReference type="GeneID" id="10276498"/>
    <dbReference type="KEGG" id="mel:Metbo_0075"/>
    <dbReference type="eggNOG" id="arCOG02573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="OMA" id="NLGATFW"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="21975" checksum="DB808A1BF4A63EBD" modified="2011-05-03" version="1">MKVLGICGSPRKQATHHVLNEALQMLDEKGFETEFYSLRGKNISPCRHCDYCMKHKECVVDDDMQDIYPLITEAEGIIMATPVYNGGLSAQLKAVMDRCRALGARDFDFLRYKVGMAIAVGGDRIGGQELAIQQIMTFFILTGAIPVSGGAYGANMGANFWSKDTLEGVKQDEEGFKSLRKTVKRFSNFIENFEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A8</accession>
    <name>F0T7A8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <sequence length="109" mass="12657" checksum="A26DF6966B24AF17" modified="2011-05-03" version="1">MHLGFLLTKTPSEEGYNTFLKFIEMYKSHDLTIYLVGNGVYNFRKNYGASEHLLELLKNSPSLKIYSCEDDFEARGIGKETLIEDIIPFKTYDTIVVDIMENQDQIFSF</sequence>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">AL-21</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1333"/>
    <dbReference type="EnsemblBacteria" id="ADZ09574">
        <property type="protein sequence ID" value="ADZ09574"/>
        <property type="gene ID" value="Metbo_1333"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1333"/>
    <dbReference type="eggNOG" id="arCOG03821">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763884_0_0_2"/>
    <dbReference type="OMA" id="WIVIKEV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16740" checksum="5F33EB519545C8F9" modified="2011-05-03" version="1">MDSKGWLSADALIAFLVLIILITSLINIVGNRIDTVNSIEEASESRVLGENIAQQIETTHSNGPGYYTIYRTPSTIFHEYYHVHINSSGLYIFVNGKTCYSYLSFMRVTGSDYMRDLEVTLKPDTSYNITSKQDTLKNTWIVIKEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P9</accession>
    <name>F0T7P9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="13">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_2518</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646082.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2518"/>
    <dbReference type="EnsemblBacteria" id="ADZ10731">
        <property type="protein sequence ID" value="ADZ10731"/>
        <property type="gene ID" value="Metbo_2518"/>
    </dbReference>
    <dbReference type="GeneID" id="10278992"/>
    <dbReference type="KEGG" id="mel:Metbo_2518"/>
    <dbReference type="eggNOG" id="arCOG02059">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_2_2"/>
    <dbReference type="OMA" id="CDDVIMD"/>
    <dbReference type="OrthoDB" id="29942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="557"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="587"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="619" mass="67488" checksum="44B49436B1EF40BB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T2</accession>
    <name>F0T7T2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Replication factor-A domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0167</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643770.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0167"/>
    <dbReference type="EnsemblBacteria" id="ADZ08419">
        <property type="protein sequence ID" value="ADZ08419"/>
        <property type="gene ID" value="Metbo_0167"/>
    </dbReference>
    <dbReference type="GeneID" id="10276593"/>
    <dbReference type="KEGG" id="mel:Metbo_0167"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019441_0_0_2"/>
    <dbReference type="OMA" id="NAFSRKW"/>
    <dbReference type="OrthoDB" id="8528at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="5">
        <location>
            <begin position="660"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="793" mass="90227" checksum="07F51383C247F557" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U1</accession>
    <name>F0T7U1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643779.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0176"/>
    <dbReference type="EnsemblBacteria" id="ADZ08428">
        <property type="protein sequence ID" value="ADZ08428"/>
        <property type="gene ID" value="Metbo_0176"/>
    </dbReference>
    <dbReference type="GeneID" id="10276602"/>
    <dbReference type="KEGG" id="mel:Metbo_0176"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478695_0_0_2"/>
    <dbReference type="OMA" id="FIYMHFP"/>
    <dbReference type="OrthoDB" id="17570at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="27"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="571" mass="63421" checksum="564A25450F7B0842" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U6</accession>
    <name>F0T7U6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="4">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">CbiXS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Sirohydrochlorin nickelchelatase</fullName>
            <ecNumber evidence="4">4.99.1.11</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbiX</name>
        <name evidence="4" type="synonym">cfbA</name>
        <name evidence="5" type="ordered locus">Metbo_0181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:52796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="EC" id="4.99.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="4"/>
    <dbReference type="EC" id="4.99.1.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643784.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0181"/>
    <dbReference type="EnsemblBacteria" id="ADZ08433">
        <property type="protein sequence ID" value="ADZ08433"/>
        <property type="gene ID" value="Metbo_0181"/>
    </dbReference>
    <dbReference type="GeneID" id="10276607"/>
    <dbReference type="KEGG" id="mel:Metbo_0181"/>
    <dbReference type="eggNOG" id="arCOG02246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="OMA" id="HEHEKLE"/>
    <dbReference type="OrthoDB" id="93992at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="78"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="16640" checksum="8A3D70F165DDBD1D" modified="2011-05-03" version="1">MDTNSSSKIKTGVILVGHGSRLPYGKDVVSQIAEIYRTKQDYMVEVGFMNMNKPSIPEAINKLAKNGAERIVVTPVFLANGVHTTEDIPKILGLKEDTHEEGHSHHGHAHSHDEEEKVEIEFDGEIIYTEPLGADERIADIINDRVNDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X8</accession>
    <name>F0T7X8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643816.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0213"/>
    <dbReference type="EnsemblBacteria" id="ADZ08465">
        <property type="protein sequence ID" value="ADZ08465"/>
        <property type="gene ID" value="Metbo_0213"/>
    </dbReference>
    <dbReference type="GeneID" id="10276639"/>
    <dbReference type="KEGG" id="mel:Metbo_0213"/>
    <dbReference type="eggNOG" id="arCOG13991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784124_0_0_2"/>
    <dbReference type="OMA" id="FMESMKE"/>
    <dbReference type="OrthoDB" id="143611at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7469" checksum="EBD2D9C45A43580C" modified="2011-05-03" version="1">MEIKATVGVCTKLKNNNEDQLVVVPKDGEMFEKNQVVLVISAAEFENLSSELQSMINMVKGAQAVDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T821</accession>
    <name>F0T821_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Drug resistance transporter, EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644998.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09647">
        <property type="protein sequence ID" value="ADZ09647"/>
        <property type="gene ID" value="Metbo_1408"/>
    </dbReference>
    <dbReference type="GeneID" id="10277859"/>
    <dbReference type="KEGG" id="mel:Metbo_1408"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_2"/>
    <dbReference type="OMA" id="PMYLQLV"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="17"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="477" mass="51544" checksum="D292D6993677E6AC" modified="2011-05-03" version="1">MNSTNTQNNLIKHYKLILSGLIISLLIVAIDSTITTTAMPKIIASLGGLKYYVWPFTIYLTTVIISAMLSGKLTDFYGSKKILIVALMVFIVGSVLCGFSKNMGELILFRALQGLGAGIIVTVPKKIIAEMFPPRQRGKYMGLFGVAGGISTVVGPTLGGFITDFLGWQWIFFVNVPIGIITLSLIIPYLPELGVIVKDKIIDYNGLITFTVALTSFLAALTFSQQSTVAPIYVELLWLVSLIMLAGFIYAEKTAKEPVLPLYLFKNSVFTVSTVSVFLLGAITLAGTVYIPVFLQLVQGMGPQISGLFLTPLMFSMIVTAILSGQIISKTGTYKKLAIVSFATGALGMFLLSTLTPSTTNLELIIYELITGIGAGLAMPIFMIAVQNAVSKRDLGVVTSSSMYFEQLGSVIGLAVLGSIMNTTLNYNLQNSTANMASTLLVTALHNVFIAALIMNLLGFVICFFLKDIYMSNEFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T880</accession>
    <name>F0T880_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4">1.5.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643843.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0240"/>
    <dbReference type="EnsemblBacteria" id="ADZ08492">
        <property type="protein sequence ID" value="ADZ08492"/>
        <property type="gene ID" value="Metbo_0240"/>
    </dbReference>
    <dbReference type="GeneID" id="10276670"/>
    <dbReference type="KEGG" id="mel:Metbo_0240"/>
    <dbReference type="eggNOG" id="arCOG00455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_2_0_2"/>
    <dbReference type="OMA" id="AKQTCLG"/>
    <dbReference type="OrthoDB" id="70966at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006561">
        <property type="term" value="P:proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="2">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="3">
        <location>
            <begin position="161"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="272" mass="30031" checksum="15CDEB16575E2D1E" modified="2011-05-03" version="1">MKKIGFIGYGSMGHVILNGFLNSGMLKPFNVTVSTRTPSKLSELEKNHPEVEIANDNIQTAQNSDIIFLFTGTSDVKYVIEEIKTSLSEKTHLIYISAALGMDLVGTIFDGKITKVIPSITSEVHEGVSLICHQESVTTEEADFVDNLFRTVGEVKLVDEVDLDVGADITSCSPAFIAKIFQEFSVQASNNSNFSKEEAEKMIISTLYGTSKLLYEKGYGVDDLISAVATKGGITEEGVKILDSELPELFSELFKTTIKKHQIIRRELEEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T886</accession>
    <name>F0T886_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643849.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08498">
        <property type="protein sequence ID" value="ADZ08498"/>
        <property type="gene ID" value="Metbo_0246"/>
    </dbReference>
    <dbReference type="GeneID" id="10276678"/>
    <dbReference type="KEGG" id="mel:Metbo_0246"/>
    <dbReference type="eggNOG" id="arCOG06761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978643_0_0_2"/>
    <dbReference type="OrthoDB" id="104690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C39" evidence="1">
        <location>
            <begin position="133"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="284" mass="30967" checksum="4E4F8535AD557C3A" modified="2011-05-03" version="1" precursor="true">MLAVLMIFGLALVMNVGSVSAANTTNTTVQSHTTVQSTVTTSKNVTTTVKKTTTKKVATNSYGLTTAQIKDGLNRAQTFYNKNNRLPNYVSYGTTKIPIASFQKILTAYGLKIVVKTYANGWVTVKNLVYDHQDTSYTCGPSSLKMELSDYGLSLNEMTLASYSSSNSNVGTTHSGLINAVKKVNSIYGTKLSAWDSKFSSVGWAGLYKYISTNHPVILHIRSFLNPNTSGHYVVLMGLNMNSGLAKIADPSYGYRTLSFNALLTRMNWVVSTGRTTKPVIFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T888</accession>
    <name>F0T888_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0248"/>
    <dbReference type="EnsemblBacteria" id="ADZ08500">
        <property type="protein sequence ID" value="ADZ08500"/>
        <property type="gene ID" value="Metbo_0248"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0248"/>
    <dbReference type="eggNOG" id="arCOG06480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848915_0_0_2"/>
    <dbReference type="OMA" id="WNTILVI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="334" mass="37127" checksum="54D71CAC7A985DF6" modified="2011-05-03" version="1" precursor="true">MHINRLSIIGLIMIVFSLVLIAVNTFDKLILELTYPFLMGSSKGKSIIFFMLMGSLLILSQLIQSSKFPKKLNIFNKPGNYYLKLLIIIISITYVVGLLIEIWIRLKFGVSIFTTFVSTSPQIGTSSIIHSHVYKSMLGILISDIGLNLPSNIHTAISIAAYIPRFAAVIFVVFPVVYILGVLSTEKRPDIQNIILIFAITTTLIGMLDGGLFSAPAMVGLAGLLGIYSIKKPFSPRDLVKPSMIIVLLLILRIIISFMGSSPDVYEITILDEHENVTVKGFNVINEMAVGNNTVITVPANMNEFTVLNTSFDVLKDKYHLFFVSWDSYSFFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T893</accession>
    <name>F0T893_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0253"/>
    <dbReference type="EnsemblBacteria" id="ADZ08505">
        <property type="protein sequence ID" value="ADZ08505"/>
        <property type="gene ID" value="Metbo_0253"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0253"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01968">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_430009_0_0_2"/>
    <dbReference type="OMA" id="ILKHGCT"/>
    <dbReference type="OrthoDB" id="11337at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02829">
        <property type="entry name" value="3H"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75500">
        <property type="entry name" value="SSF75500"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="124"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="646" mass="72895" checksum="65413C8E0226EB69" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B9</accession>
    <name>F0T8B9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide pyruvyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643882.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0279"/>
    <dbReference type="EnsemblBacteria" id="ADZ08531">
        <property type="protein sequence ID" value="ADZ08531"/>
        <property type="gene ID" value="Metbo_0279"/>
    </dbReference>
    <dbReference type="GeneID" id="10276711"/>
    <dbReference type="KEGG" id="mel:Metbo_0279"/>
    <dbReference type="eggNOG" id="arCOG04826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637152_0_0_2"/>
    <dbReference type="OMA" id="VEGSCYM"/>
    <dbReference type="OrthoDB" id="48792at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PS_pyruv_trans" evidence="1">
        <location>
            <begin position="63"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="50033" checksum="65C65E04A63DFBB1" modified="2011-05-03" version="1">METEKNKLKDNTNSKKFKKVLLMGYNGANNTGSETRLISIIEDIRTVLGKDVKLTIPTLNEKNLRRYIEEDEHLKIVPIPSIYFFALKEIVKKQDLVLLVEGSCYMDTWTSALLWAFLWTTRCAYNQGIPCIAYAVDSGELSRFNKFLVKKEANKTDLIITRTTYARERLIDIGVKAPIVSTADCAFTFETKLEAKNSLKKIWNSENPASTEGFVGMAVVDFHLWPVVIRPWGRKVNLYKWPYYFSRSRSRVLRSENLSRNWAREADRIVELHSKNIIIICMEELDEPLAIEIKNRMKHSEKAQIVSAGTYNASQITEVLRSLELLITSRYHAGVLSMKASVPQIGIGHDQRLKGLYKDIKIEKELFIDYPEDNSKVEVIWKSLSKKVDQLIENPKHTKELIRKCYQKQVELSLNNKKYLKNFLFEHEDVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C7</accession>
    <name>F0T8C7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RecB family exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643890.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0287"/>
    <dbReference type="EnsemblBacteria" id="ADZ08539">
        <property type="protein sequence ID" value="ADZ08539"/>
        <property type="gene ID" value="Metbo_0287"/>
    </dbReference>
    <dbReference type="GeneID" id="10276719"/>
    <dbReference type="KEGG" id="mel:Metbo_0287"/>
    <dbReference type="eggNOG" id="arCOG00793">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006891_0_0_2"/>
    <dbReference type="OMA" id="MISEFMF"/>
    <dbReference type="OrthoDB" id="84826at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_Cas4" evidence="1">
        <location>
            <begin position="149"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="268" mass="30971" checksum="420F5715DB8ECE8C" modified="2011-05-03" version="1">MITVTNIKEYLYCPEKLNLRLENLDITTEEQISGKISKEAFKGFDSIVQRNLWVLNGKMNIRDILNELFKDMPSFLDTIYRRYQEEGIEDPSPIFESLKEDMRFNSWLIAIKTQKLLNDGINGSEAVKILYPPSFIEFKIENPEVGLVGMIDKIEIIDGTYYPIAIKTNLPPLNGVWESDAIQSSAYSFLMEEEFNKDVAVGFINYTKIGSKQPVVNSPLLTNKFIEIFEKLCDMIYEGKKPEIHININKCRLCEYSTMCSYCNQHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D3</accession>
    <name>F0T8D3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="5">CDG synthase</shortName>
            <ecNumber evidence="5">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">queE</name>
        <name evidence="7" type="ordered locus">Metbo_0293</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0293"/>
    <dbReference type="EnsemblBacteria" id="ADZ08545">
        <property type="protein sequence ID" value="ADZ08545"/>
        <property type="gene ID" value="Metbo_0293"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0293"/>
    <dbReference type="eggNOG" id="arCOG02173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_1_0_2"/>
    <dbReference type="OMA" id="IPQTHKM"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="18"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="26264" checksum="80AE53A46F7E5585" modified="2011-05-03" version="1">MKTNINEVFSSFQGEGTLIGRRQIFVRFSGCNLDCSYCDTPESRNPQYGELISTDELYSKVNSIITPDFHSISFTGGEPLLHSNFIKKFLEEYEFKSMLETNGTLPAELNKIAHLLDYVSLDIKLPEHGASNNYADILDLELKSIKLLRDEEINTYIKVVVMPQTEVELISSIASRIADEVEKTSELVMVLQPVSPITVWADGTQKLFELSEVAGKYIDVLTIPQVHKLLNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E2</accession>
    <name>F0T8E2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-oxoacid:acceptor oxidoreductase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1456</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645044.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1456"/>
    <dbReference type="EnsemblBacteria" id="ADZ09693">
        <property type="protein sequence ID" value="ADZ09693"/>
        <property type="gene ID" value="Metbo_1456"/>
    </dbReference>
    <dbReference type="GeneID" id="10277907"/>
    <dbReference type="KEGG" id="mel:Metbo_1456"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OMA" id="AGYYIYG"/>
    <dbReference type="OrthoDB" id="41678at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
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        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
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        <location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01855"/>
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    </evidence>
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        <source>
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        <source>
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    <sequence length="573" mass="63678" checksum="06A7F41EB26527AA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="38" xmlns="http://uniprot.org/uniprot">
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        </submittedName>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1478"/>
    <dbReference type="EnsemblBacteria" id="ADZ09712">
        <property type="protein sequence ID" value="ADZ09712"/>
        <property type="gene ID" value="Metbo_1478"/>
    </dbReference>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="648" mass="75556" checksum="47AA426CFDB270E1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K6</accession>
    <name>F0T8K6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1527"/>
    <dbReference type="EnsemblBacteria" id="ADZ09757">
        <property type="protein sequence ID" value="ADZ09757"/>
        <property type="gene ID" value="Metbo_1527"/>
    </dbReference>
    <dbReference type="GeneID" id="24964561"/>
    <dbReference type="KEGG" id="mel:Metbo_1527"/>
    <dbReference type="eggNOG" id="arCOG02879">
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    </dbReference>
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    <dbReference type="OrthoDB" id="101775at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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            <end position="41"/>
        </location>
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            <end position="219"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="439" mass="48610" checksum="9ECCFED78294E339" modified="2011-05-03" version="1">MNLKEVVIDAVKYPFLSVKNVLILFIFILIGFFVVLTSFFVNGYGYRIIKSSIVGEDEPPVFNRLGGMFLDGVKVFMVSFGYAIPVVVMIIIYLAAFVSSVMSYPNNTSIFVKFGGVGIVVAIVSTVYSIIITPIFAMALVYMADNNDQFSAAFRFREILNKIKNLGWKKLIIWYLTILLASIITIELVVVSTFISNMLNLPVQLLSAFIFTILFMYICRSTALFYTSDSPGYLVCEQCGGYYKLKSGESPEDFDTCECGGKLIYAASNPNLNESEESITNETVNRGIFGLSKIKKNKDSIILIGIVVLSILAIPVLIHSTQNQTPTNYTLLASYNVNNLTNGSKGVNIPQGTKNIKVDYNISWVDPGDLSSYFNLNAYDVYIGEGATAGMLNRVSNKELNLKKGQNKTGTYYFNKPPVKSIKLYGNGIQGTVNIYTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q0</accession>
    <name>F0T8Q0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643943.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0340"/>
    <dbReference type="EnsemblBacteria" id="ADZ08592">
        <property type="protein sequence ID" value="ADZ08592"/>
        <property type="gene ID" value="Metbo_0340"/>
    </dbReference>
    <dbReference type="GeneID" id="10276777"/>
    <dbReference type="KEGG" id="mel:Metbo_0340"/>
    <dbReference type="eggNOG" id="arCOG05509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187355_0_0_2"/>
    <dbReference type="OMA" id="MYLIFRC"/>
    <dbReference type="OrthoDB" id="123631at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015166">
        <property type="entry name" value="DUF1922"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036304">
        <property type="entry name" value="MTH1184"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09082">
        <property type="entry name" value="DUF1922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57821">
        <property type="entry name" value="SSF57821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="74" mass="8378" checksum="2EA7C237BBD98D4D" modified="2011-05-03" version="1">MYIVFRCDCGRAVYSKQGTATKKCVCGKTLKVKERRILAMTEEAEQASDKVRELQEEKYGGAIFTTADKVPNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q2</accession>
    <name>F0T8Q2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TraB family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643945.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0342"/>
    <dbReference type="EnsemblBacteria" id="ADZ08594">
        <property type="protein sequence ID" value="ADZ08594"/>
        <property type="gene ID" value="Metbo_0342"/>
    </dbReference>
    <dbReference type="GeneID" id="10276779"/>
    <dbReference type="KEGG" id="mel:Metbo_0342"/>
    <dbReference type="eggNOG" id="arCOG02142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032780_1_0_2"/>
    <dbReference type="OMA" id="WMLSYVQ"/>
    <dbReference type="OrthoDB" id="52124at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005230">
        <property type="entry name" value="TraB_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PTHR21530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00261">
        <property type="entry name" value="traB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="384" mass="42589" checksum="7033AA254C95A5C2" modified="2011-05-03" version="1">MAPENLKLIGTAHVSKESIEEVRAAIIENQPKVVAVELDLNRFQNMMAERNGQEKQDINLREIIKGDKLSIFLVSMFLSYMQRKIGDKLGVKPGSEMLAAVDTAEEIGANVALIDRDISLTLKRAIEKMSWIEKAKFLYGIITSFFSSEEIDDVESIKDGDELTEVMEYFKEMSPKAYDVLVSERDAYMSHRLLEINNESVVAVVGAGHKKGITRYMEHPEELPPIEDLLTLKKSRIPIGKIILYSIPLVFVIIFALAILHGINVQTSIINFILLTGTFSFLGCILSGSKIYSAITAFVVAPLTTIHPLLAAGWFSGIVEAKMRHISMDDAVEISKVDSFRELWGNNLFRVLLVVVGTNIGASIGLFLTIPNVVLPLFGKIFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T966</accession>
    <name>F0T966_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">V-type ATP synthase subunit F</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">V-ATPase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpF</name>
        <name evidence="7" type="ordered locus">Metbo_0436</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644039.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0436"/>
    <dbReference type="EnsemblBacteria" id="ADZ08688">
        <property type="protein sequence ID" value="ADZ08688"/>
        <property type="gene ID" value="Metbo_0436"/>
    </dbReference>
    <dbReference type="GeneID" id="10276873"/>
    <dbReference type="KEGG" id="mel:Metbo_0436"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_2_0_2"/>
    <dbReference type="OMA" id="QIPDKFG"/>
    <dbReference type="OrthoDB" id="113093at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="SSF159468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11730" checksum="2E0A359CF6A9623E" modified="2011-05-03" version="1">MNSNIAVMADEDIVTGFMLGGIKEGHPVKDMDEAEKTLKELVSRNFSVIITTEKIGDALRKTINKVTNESALPMIIEIPDKTGSIRRESDPMSELIKRVIGVEMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T986</accession>
    <name>F0T986_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1610"/>
    <dbReference type="EnsemblBacteria" id="ADZ09837">
        <property type="protein sequence ID" value="ADZ09837"/>
        <property type="gene ID" value="Metbo_1610"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1610"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OMA" id="NEMSHYS"/>
    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10964" checksum="1AC8FBB66953CBC8" modified="2011-05-03" version="1">MKGYLTFLILWTLKKSEMNGAEISREFEKRRGTKPSPGTIYPALKELRKNELITVDEAKTYALTEKGELELKSACKFFCKIFYDVNEMSHYSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F4</accession>
    <name>F0T9F4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645256.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1679"/>
    <dbReference type="EnsemblBacteria" id="ADZ09905">
        <property type="protein sequence ID" value="ADZ09905"/>
        <property type="gene ID" value="Metbo_1679"/>
    </dbReference>
    <dbReference type="GeneID" id="10278136"/>
    <dbReference type="KEGG" id="mel:Metbo_1679"/>
    <dbReference type="eggNOG" id="arCOG00734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085608_0_0_2"/>
    <dbReference type="OMA" id="GDYMITE"/>
    <dbReference type="OrthoDB" id="85881at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016723">
        <property type="entry name" value="Tscrpt_reg_ArsR_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018357">
        <property type="entry name" value="Trans_reg_ArsR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="169"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="29030" checksum="F4639C39F37A8DB7" modified="2011-05-03" version="1">MVNVDEYEVMTTSNVNEKLDKIHQDIKRLMEKSNQDYLELMLNNLRKDFTRSIITYLKDDAQTRLDNNMEICEMKPTCKEVFTDFLNGNTELLKNNNATETSIKEKKDELARIKDKAPYTKCETCFSEVQTLFNQQLNLIRSNDIYNSDSAQDEKPKISSIHEKKLVKEVLDPISNKQRLQILKEMSSKAQSFSALSELTGLRGGNLLFHIQKLQDHDLIIQRHERGDYMITNKGFNILMLLAEFQKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H0</accession>
    <name>F0T9H0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit delta</fullName>
            <shortName evidence="1">ACDS complex subunit delta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhD</name>
        <name evidence="3" type="ordered locus">Metbo_0466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). Probably maintains the overall quaternary structure of the ACDS complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644069.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0466"/>
    <dbReference type="EnsemblBacteria" id="ADZ08718">
        <property type="protein sequence ID" value="ADZ08718"/>
        <property type="gene ID" value="Metbo_0466"/>
    </dbReference>
    <dbReference type="GeneID" id="10276904"/>
    <dbReference type="KEGG" id="mel:Metbo_0466"/>
    <dbReference type="eggNOG" id="arCOG01980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040403_0_0_2"/>
    <dbReference type="OMA" id="VMDPTTC"/>
    <dbReference type="OrthoDB" id="72026at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01135">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004486">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00381">
        <property type="entry name" value="cdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdhD" evidence="2">
        <location>
            <begin position="75"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="386" mass="42977" checksum="032A68EAFE1E46B1" modified="2011-05-03" version="1">MDKMTQLLKLLENTDTIEINDFRMDFDELEIQIMPAVQRMVQQVAQKQAEVAREVLPTMDPFAPPVHNWPGEVAEVQLGAGSRKSVFLGGQKALYRFEEPQPNPPVVTFDVFDIPMPGLPKPIREHFGDVMEHPGEWAKKAVKDYGANMITIHLIGTGPKVMDKTPRQAAQDIEEVLQAVKVPLVIGASGDPVKDPIVLEAAAQAAEGERCLLASANLDLDYKRVAKAAVDYNHAVLSWAITDVNMQKTLNKYLMKEGLTQNDIVMDPTTCALGYGIEFSIDVITRTRLAALKGDTDLQMPMSSGTTNAWGSREAWMKKDEWGPTAYRGPIWEIITGLTMMLCGVDIFMMLHPSSVKMLKEIGNTFTRDYLSTDVPDITNWITELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H6</accession>
    <name>F0T9H6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644075.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0472"/>
    <dbReference type="EnsemblBacteria" id="ADZ08724">
        <property type="protein sequence ID" value="ADZ08724"/>
        <property type="gene ID" value="Metbo_0472"/>
    </dbReference>
    <dbReference type="GeneID" id="10276910"/>
    <dbReference type="KEGG" id="mel:Metbo_0472"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_6_2"/>
    <dbReference type="OMA" id="MEVQSEN"/>
    <dbReference type="OrthoDB" id="123760at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="58" mass="6035" checksum="879C211512C7B851" modified="2011-05-03" version="1">MEVQSENCGYCGSCVTVCPEGIIELAENGLNVEIGCTDCGNCVYVCPLGALMVGKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I7</accession>
    <name>F0T9I7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0483</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644086.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0483"/>
    <dbReference type="EnsemblBacteria" id="ADZ08735">
        <property type="protein sequence ID" value="ADZ08735"/>
        <property type="gene ID" value="Metbo_0483"/>
    </dbReference>
    <dbReference type="GeneID" id="10276923"/>
    <dbReference type="KEGG" id="mel:Metbo_0483"/>
    <dbReference type="eggNOG" id="arCOG00232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_2_0_2"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="OrthoDB" id="76473at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="3">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="3">
        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="234" mass="27012" checksum="6A8A626D175D97F7" modified="2011-05-03" version="1">MKKYPRIQFQKRLDQLRNDVEEMGQTSLDSYKNAIDAFKNYDSELVNKVIRNHQKLQEMNYNLEQEAMSIIASEQPVAKDLRFIETSIKVASHLKRMGGLASNIADVAEHIKDEEIPEKPMNDITHMSDIVDGMVSKSLSAFLNEDMNLVRELKRDDDKVDDLFDQALNDISNSMFQEKEAISYMISLLFVARFLERIADRAENIGDRTIYMITCEKPGVADKLNQLINDEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J4</accession>
    <name>F0T9J4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate/ketoisovalerate oxidoreductase, gamma subunit</fullName>
            <ecNumber evidence="3">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644093.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0490"/>
    <dbReference type="EnsemblBacteria" id="ADZ08742">
        <property type="protein sequence ID" value="ADZ08742"/>
        <property type="gene ID" value="Metbo_0490"/>
    </dbReference>
    <dbReference type="GeneID" id="10276930"/>
    <dbReference type="KEGG" id="mel:Metbo_0490"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="OMA" id="KWGQAIP"/>
    <dbReference type="OrthoDB" id="95610at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="10"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="177" mass="18922" checksum="36389ABBC76A7C91" modified="2011-05-03" version="1">MIEVRFHGRGGQGAVTAAEILAKAAFEDGKYCQAFPFFGVERRGAPVMAFTRIDDKPIRRRYQVYNPDYVVVLDDGLLEVVDVFSGIKEGGNVIINTNNGVENSNGVKINKIDATGIALDILGVPIVNTILLGAFAGVTGQVSIESLIKIINETFSGSVAEKNVKAAKMAYDAAKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K9</accession>
    <name>F0T9K9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF815 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0505</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644108.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0505"/>
    <dbReference type="EnsemblBacteria" id="ADZ08757">
        <property type="protein sequence ID" value="ADZ08757"/>
        <property type="gene ID" value="Metbo_0505"/>
    </dbReference>
    <dbReference type="GeneID" id="10276945"/>
    <dbReference type="KEGG" id="mel:Metbo_0505"/>
    <dbReference type="eggNOG" id="arCOG05101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039512_1_1_2"/>
    <dbReference type="OMA" id="FIIYMDD"/>
    <dbReference type="OrthoDB" id="16939at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF815" evidence="2">
        <location>
            <begin position="185"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="438" mass="50522" checksum="95827BE537389494" modified="2011-05-03" version="1">MYHLTSKLVIYKNINEDSILFRLAAICQQLDNGNYVREDLISDIYIEINRLLDLSTQYGFDKNLWHNYLAFILAMAQNPFTIVSEKVGTNTGTVNKFVKNDYKIFKQLFDYDFSKLENELGIDCFSIITDYDAVVKSEQIFNKNVSEKVQQLSNSIEQAKDEKELYKIVTDFYKLHGVGDIGLNKAFRVSTDGNSSILCPITTTSDIKLEDLVGYESQKKELLQNTEAFVIGRKANNVLLYGDAGTGKSASIKAVLNQYYDQGLRMIEVYKHEFKELPKIIDVIKNRNYRFIIYMDDLSFEEFEIEYKYLKAVIEGGLESKPENVLIYATSNRRHLIRETWSDRSDMSEDELHRSDTVNEKVSLAERFGVKIGYYNPQRKDFFNIVTTLARRHPEIKLTDEELESLANKWEIRHGGMSGRTAQQFIDSLLGTVGNPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M1</accession>
    <name>F0T9M1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein family UPF0066</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0517</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0517"/>
    <dbReference type="EnsemblBacteria" id="ADZ08769">
        <property type="protein sequence ID" value="ADZ08769"/>
        <property type="gene ID" value="Metbo_0517"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0517"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_0_2"/>
    <dbReference type="OMA" id="QIQYFAR"/>
    <dbReference type="OrthoDB" id="117039at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15570" checksum="93E968D7D1D6B4D9" modified="2011-05-03" version="1">MDIKLEPIGIIRSPYTNKNNSPRQGRYSNETSVLTVYKKYMDGIEGIDLHSHYMIFYWQDHAIRNQLKVVPHGKTEKRGVFSTRAPVRPNPIGFCLVEVVNIEDNQITVRWLDAWDGSPLLDIKPYWPEIDTPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M9</accession>
    <name>F0T9M9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645259.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09908">
        <property type="protein sequence ID" value="ADZ09908"/>
        <property type="gene ID" value="Metbo_1682"/>
    </dbReference>
    <dbReference type="GeneID" id="10278139"/>
    <dbReference type="KEGG" id="mel:Metbo_1682"/>
    <dbReference type="HOGENOM" id="CLU_1485893_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="181" mass="20243" checksum="8DA10BF70F5A9944" modified="2011-05-03" version="1" precursor="true">MDKKDLILILAVFSVLVMCSTVFATGNVNSSKISSSTNLGNSKYPKLIDAGVTLTKGYGVKKNSAGHYSIGIADKIYPGMNKPTKYYWKTYLYQNGTIVIYTHFYHTTLKKEIYQTIVIGNYATKNGNIVYHSVSPKSWGASSYKTLDGLWSTPLQFYWNKSNGYPSFRERMINNAPTGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P1</accession>
    <name>F0T9P1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645271.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09920">
        <property type="protein sequence ID" value="ADZ09920"/>
        <property type="gene ID" value="Metbo_1695"/>
    </dbReference>
    <dbReference type="GeneID" id="10278152"/>
    <dbReference type="KEGG" id="mel:Metbo_1695"/>
    <dbReference type="eggNOG" id="arCOG10450">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193452_0_0_2"/>
    <dbReference type="OMA" id="SYVEIMM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="83" mass="9405" checksum="C2CE134814475FC9" modified="2011-05-03" version="1">MSFDITTTLNLCFDLIIVVLGLLAFKRKQYVLALYVALGFAMFAFSYVEIMMGLSSQNISVVFLRILGYLVIIYALLKEVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R4</accession>
    <name>F0T9R4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645294.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1719"/>
    <dbReference type="EnsemblBacteria" id="ADZ09943">
        <property type="protein sequence ID" value="ADZ09943"/>
        <property type="gene ID" value="Metbo_1719"/>
    </dbReference>
    <dbReference type="GeneID" id="10278176"/>
    <dbReference type="KEGG" id="mel:Metbo_1719"/>
    <dbReference type="eggNOG" id="arCOG04946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127592_3_1_2"/>
    <dbReference type="OMA" id="PRVIHFE"/>
    <dbReference type="OrthoDB" id="114177at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="14236" checksum="A2B428C57C3912B6" modified="2011-05-03" version="1">MSRVIWFEIPADDPERAAKFYEDVFGWEIEKWEGPFDYWLIRTGSDEEPGIHGAIMTKDMGEVVRDTIAVDSYDEFSKKIEMQGGEMLTEKIEVPNMGYMGSFKDTEGNIFAIIESNMTETSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W5</accession>
    <name>F0T9W5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="4">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name evidence="5" type="ordered locus">Metbo_0536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644139.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0536"/>
    <dbReference type="EnsemblBacteria" id="ADZ08788">
        <property type="protein sequence ID" value="ADZ08788"/>
        <property type="gene ID" value="Metbo_0536"/>
    </dbReference>
    <dbReference type="GeneID" id="10276981"/>
    <dbReference type="KEGG" id="mel:Metbo_0536"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OMA" id="MWGGGMM"/>
    <dbReference type="OrthoDB" id="61905at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="55"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28107" checksum="DEA7E653A05A54CB" modified="2011-05-03" version="1">MKLDDIIVSKAIIEEYMQDFLDYTDMDVAIGGGGPAGLTAGYYLAKAGYKVALFEKKLSMGGGMWGGGMMFNKIVVQEESKRILDEFGIKTKEYSEGYFVADSIECVSTICSKAVQAGLKIFNLMAIEDVMMKDKGVEGVVLNWAAVQMGGLHVDPLTVRSKAVIDATGHPCEVVKILENKMEVELETETGKIMGEKSMWADKAESLVVDNTTEVYPGLYVTGMAANAVHGSPRMGPIFGGMLLSGERVAEVIIEKFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA39</accession>
    <name>F0TA39_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645340.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1766"/>
    <dbReference type="EnsemblBacteria" id="ADZ09989">
        <property type="protein sequence ID" value="ADZ09989"/>
        <property type="gene ID" value="Metbo_1766"/>
    </dbReference>
    <dbReference type="GeneID" id="10278223"/>
    <dbReference type="KEGG" id="mel:Metbo_1766"/>
    <dbReference type="HOGENOM" id="CLU_2447697_0_0_2"/>
    <dbReference type="OrthoDB" id="140273at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10306" checksum="44ED06317E1976E7" modified="2011-05-03" version="1">MSTKKQPLRSTTGRVDDSETVKLLDGSHDLKPDDVVVVIHAEDYQKTFQKMLERINEMDKKVDDQSKILKESADQEKKGFIGRFRKPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA60</accession>
    <name>F0TA60_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645361.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1788"/>
    <dbReference type="EnsemblBacteria" id="ADZ10010">
        <property type="protein sequence ID" value="ADZ10010"/>
        <property type="gene ID" value="Metbo_1788"/>
    </dbReference>
    <dbReference type="GeneID" id="10278245"/>
    <dbReference type="KEGG" id="mel:Metbo_1788"/>
    <dbReference type="eggNOG" id="arCOG00528">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137964_2_1_2"/>
    <dbReference type="OMA" id="EAYFWTM"/>
    <dbReference type="OrthoDB" id="89010at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16200" checksum="69E93BDFC41B653C" modified="2011-05-03" version="1">MNYDDCIKFANDTPVCYLASAEGDQPRVRALGFWFADETGFYFQIGAVKDMYAQLQANPKVEVCFWQPGEYSGTMMRVAGEVEFVDDPELKKKVLEDRPFLKEFGMTFDHPGLVIFRIPSGEAYFWTMESNFEPKKMIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA68</accession>
    <name>F0TA68_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645369.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10018">
        <property type="protein sequence ID" value="ADZ10018"/>
        <property type="gene ID" value="Metbo_1796"/>
    </dbReference>
    <dbReference type="GeneID" id="10278253"/>
    <dbReference type="KEGG" id="mel:Metbo_1796"/>
    <dbReference type="HOGENOM" id="CLU_2519777_0_0_2"/>
    <dbReference type="OrthoDB" id="141305at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9707" checksum="55CDDC50F06F87B5" modified="2011-05-03" version="1">MENKKTAGKKLLDSIKDRRKMFHSKARFPNAILINSNDYSVLKDFINIDTKESLTQILDLAIFISDDVPSFQLIRTYDHDDCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA84</accession>
    <name>F0TA84_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LemA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1812</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645385.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1812"/>
    <dbReference type="EnsemblBacteria" id="ADZ10034">
        <property type="protein sequence ID" value="ADZ10034"/>
        <property type="gene ID" value="Metbo_1812"/>
    </dbReference>
    <dbReference type="GeneID" id="10278269"/>
    <dbReference type="KEGG" id="mel:Metbo_1812"/>
    <dbReference type="eggNOG" id="arCOG04574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_0_1_2"/>
    <dbReference type="OMA" id="VETQYQR"/>
    <dbReference type="OrthoDB" id="78720at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="183" mass="20793" checksum="09393DC31B313E8D" modified="2011-05-03" version="1">MIIEIIIVLILIVIVVAVILLYNGLVNLRNRVKNAWSQIDVQLKRRTDLIPNLVETVKGYASHEKGVFENVTKARSSLMNASGVQESAEANNQLTGALKSLFAVAENYPDLKASQNFRELQAQLTETEDKIAYSRQFYNDTVLRYNNKVQMFPSNILASLFKFNEAEFFEMAESERAVPEVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC4</accession>
    <name>F0TAC4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644227.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0625"/>
    <dbReference type="EnsemblBacteria" id="ADZ08876">
        <property type="protein sequence ID" value="ADZ08876"/>
        <property type="gene ID" value="Metbo_0625"/>
    </dbReference>
    <dbReference type="GeneID" id="10277070"/>
    <dbReference type="KEGG" id="mel:Metbo_0625"/>
    <dbReference type="eggNOG" id="arCOG00893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="OMA" id="CAVQLHT"/>
    <dbReference type="OrthoDB" id="47746at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="PTHR42206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="281" mass="31234" checksum="22D63092193A98D2" modified="2011-05-03" version="1">MDKIPVTDNHIHVDPVNGEGPLETANKFSRAGGSFMIVPNKPTWTVNTTDYKEAMELVIKYVNEINTETDVKAFPVVGVHPAELSRLIKAGMDVSQSEQKIKNALEIAQKLVLDGEAVAIGEVGRPHYEVSPEELDAQNRLLVYAMELAKEANCPVQLHTESAGPKQYLEFAEMADRVGLPRNKVIKHFSGPFVLEEENHGLLPSLIATKDVVKTGLKKGKNILMETDYLDDKTRPGAVLGPKTVPRRTKEMIDQGLLSEDDAYRIHVENVEKVYNLNLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD1</accession>
    <name>F0TAD1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="14" type="ordered locus">Metbo_0632</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644234.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0632"/>
    <dbReference type="EnsemblBacteria" id="ADZ08883">
        <property type="protein sequence ID" value="ADZ08883"/>
        <property type="gene ID" value="Metbo_0632"/>
    </dbReference>
    <dbReference type="GeneID" id="10277077"/>
    <dbReference type="KEGG" id="mel:Metbo_0632"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_4_0_2"/>
    <dbReference type="OMA" id="NAFAFGR"/>
    <dbReference type="OrthoDB" id="62484at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="12">
        <location>
            <begin position="74"/>
            <end position="318"/>
        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
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        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ08883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="319" mass="35676" checksum="221463F1A2339ACC" modified="2011-05-03" version="1">MVRSSTLLLKLRLFLAPVILFAILYLILALVLSYFHIGYTYIYVGLAILLVLVQYLLGPKMVESSMRVHYVSEQEAPKLHAMITDLAMKAGVPKPKVGISEINVPNAFAFGRTKKDGRICVTRGILRILDEGELEAVLGHEMSHIKHSDMIVMTLISVVPLICYYIFISMLFQDRDNGYGMIIGLGALGAYLVGQLLVLFISRTREYYADQGSVDLGCKPHNLASALYKLVYGSATSNKDEIKEVEGYKAFFVNDVTDARNEINDLQQLDLNMDGKISEEELSRLRYSDTKVGTGQSVMELLSTHPNMVKRVKRLSELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI5</accession>
    <name>F0TAI5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate 4-decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ10057">
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    </dbReference>
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    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038911_0_0_2"/>
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    </dbReference>
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        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="171"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="546" mass="62320" checksum="28163D4A0CE0ED72" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ4</accession>
    <name>F0TAJ4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter, ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="Metbo_1844"/>
    </dbReference>
    <dbReference type="GeneID" id="10278301"/>
    <dbReference type="KEGG" id="mel:Metbo_1844"/>
    <dbReference type="eggNOG" id="arCOG01746">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021720_2_0_2"/>
    <dbReference type="OMA" id="IRDRRMQ"/>
    <dbReference type="OrthoDB" id="18379at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019195">
        <property type="entry name" value="ABC_ATPase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149">
        <property type="entry name" value="PTHR38149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09818">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="569" mass="63749" checksum="7F925EFCE207CCFB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK6</accession>
    <name>F0TAK6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1857"/>
    <dbReference type="EnsemblBacteria" id="ADZ10078">
        <property type="protein sequence ID" value="ADZ10078"/>
        <property type="gene ID" value="Metbo_1857"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1857"/>
    <dbReference type="eggNOG" id="arCOG01792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052868_1_1_2"/>
    <dbReference type="OMA" id="RGSYVGC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="PTHR43675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="80"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="28187" checksum="F99E789B44AC2EC9" modified="2011-05-03" version="1">MIEGVTMESNEIKEHIKDRYSKIAIKKSDNDSCTSCSVNSVIEQAKSAGYSVEDLKSIPENAMYGLGCGNPTALVELKPGDVVLDLGSGGGIDVFLAANKVGESGKVIGIDMTEEMVRNATKNALEGKYENVEFKLGEIEDLPIETDSIDVIISNCVINLTPDKLVAYKEAFRVLKPGGKILVSDLVTEEELPKNIMQKFQAWSECSAGAVELEKYLNTIKEAGFNSIEIVEQHYFTDTNLDERLVGKIISIQVKATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP6</accession>
    <name>F0TAP6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetate/CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1898</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645469.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1898"/>
    <dbReference type="EnsemblBacteria" id="ADZ10118">
        <property type="protein sequence ID" value="ADZ10118"/>
        <property type="gene ID" value="Metbo_1898"/>
    </dbReference>
    <dbReference type="GeneID" id="10278356"/>
    <dbReference type="KEGG" id="mel:Metbo_1898"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OMA" id="AIKASWP"/>
    <dbReference type="OrthoDB" id="24323at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="6">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="4">
        <location>
            <begin position="91"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="5">
        <location>
            <begin position="532"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="633" mass="71898" checksum="723EB0309BA051FA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ0</accession>
    <name>F0TAQ0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1902"/>
    <dbReference type="EnsemblBacteria" id="ADZ10122">
        <property type="protein sequence ID" value="ADZ10122"/>
        <property type="gene ID" value="Metbo_1902"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1902"/>
    <dbReference type="eggNOG" id="arCOG10883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870751_0_0_2"/>
    <dbReference type="OrthoDB" id="143392at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="15346" checksum="EC098239D19C5E59" modified="2011-05-03" version="1">MVTNEQIKLRLRNKRDGILSEGYLVCDNCGGFYELQPGEKIEDFNCNCDCGGTLKYFKQNPYPPNNITEQEPTSTLAYVGYVSIIFFALASIVIGIILYRRGGNDKQHGILILIISSVLVLPVLLISMLIIYRTYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS8</accession>
    <name>F0TAS8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP037052</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644305.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0703"/>
    <dbReference type="EnsemblBacteria" id="ADZ08954">
        <property type="protein sequence ID" value="ADZ08954"/>
        <property type="gene ID" value="Metbo_0703"/>
    </dbReference>
    <dbReference type="GeneID" id="10277148"/>
    <dbReference type="KEGG" id="mel:Metbo_0703"/>
    <dbReference type="eggNOG" id="arCOG04906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507380_0_0_2"/>
    <dbReference type="OMA" id="QWVVLSE"/>
    <dbReference type="OrthoDB" id="92769at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017098">
        <property type="entry name" value="UCP037052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09869">
        <property type="entry name" value="DUF2096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037052">
        <property type="entry name" value="UCP037052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="180" mass="21022" checksum="9C40ADCAD1068956" modified="2011-05-03" version="1">MNDLPLEQTWMVLVELLTDLRKKNVEIDPSIPEDIRMAKTTINFYKVNPADPERMKELKRINDFINAVQDKLLDLAENEGKEYRLKWIEKLTKASRGEKIYDAHQEKSKFVVGAPPGFSMVRITFKAPIHEERLQEIAEYHNVIIEFQTDTLIVIYGDKANIQESLKEISSFFKEQIEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW9</accession>
    <name>F0TAW9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit</fullName>
            <ecNumber evidence="4">4.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0744</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644346.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0744"/>
    <dbReference type="EnsemblBacteria" id="ADZ08995">
        <property type="protein sequence ID" value="ADZ08995"/>
        <property type="gene ID" value="Metbo_0744"/>
    </dbReference>
    <dbReference type="GeneID" id="10277191"/>
    <dbReference type="KEGG" id="mel:Metbo_0744"/>
    <dbReference type="eggNOG" id="arCOG04406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098588_0_0_2"/>
    <dbReference type="OMA" id="IYHCGPL"/>
    <dbReference type="OrthoDB" id="88282at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="2"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="19683" checksum="F10B5E55632D43E7" modified="2011-05-03" version="1">MNVKLKTPISSEETSNLRIGDIVYISGTIFTARDSAHKRIIEEGAPLDIEGAVIFHAGPIIKKTDDGYDMVAVGPTTSTRMNPYEPEVINMGAGLIVGKGGMDEETAKALKNNKKAYLSAVGGCAALYVKSILKINNVNWLDLGVPEAIWELEVKDFGPLIVTMDSQGGNLYDEVRKQGNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB19</accession>
    <name>F0TB19_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="4">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="4">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-hydroxybenzoate + 5-phospho-alpha-D-ribose 1-diphosphate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645516.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1945"/>
    <dbReference type="EnsemblBacteria" id="ADZ10165">
        <property type="protein sequence ID" value="ADZ10165"/>
        <property type="gene ID" value="Metbo_1945"/>
    </dbReference>
    <dbReference type="GeneID" id="10278405"/>
    <dbReference type="KEGG" id="mel:Metbo_1945"/>
    <dbReference type="eggNOG" id="arCOG01026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="OMA" id="RLNGAWY"/>
    <dbReference type="OrthoDB" id="47243at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="PTHR20861:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="5">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="6">
        <location>
            <begin position="230"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="35438" checksum="BE1EEC550EE97918" modified="2011-05-03" version="1">MIVETPSRLHITLIDLNGVHGRIDGGVGLTIDDPCIQIEAEPADNGISTIFSNKSQMIPGLLEDYSKKIEGAASRVIQARKYTEGFEFKVDAAYPPHSGLGSGTQLSLAVAKLMTELNGESLPAHELAKIVGRGGTSGIGVESFENGGFIIDGGHNSHDKSSFLPSSASSASPPPIIARYNFPEDWKVVLTIPDVEKGVSGSKEIDAFQKHCPIKLEEVEKLSHLILMKLMPAVVESDLDSFGSAINIIQNTGFKKIENKLQSVHIKRIMEELRNSGAAAVGMSSFGPTIYSVTDTNVKTVVKEAKNSIKDIGGQVIQTQARNHGAKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB28</accession>
    <name>F0TB28_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8 10">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 8 10">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisA</name>
        <name evidence="11" type="ordered locus">Metbo_1954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8 9">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 8 10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645525.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1954"/>
    <dbReference type="EnsemblBacteria" id="ADZ10174">
        <property type="protein sequence ID" value="ADZ10174"/>
        <property type="gene ID" value="Metbo_1954"/>
    </dbReference>
    <dbReference type="GeneID" id="10278414"/>
    <dbReference type="KEGG" id="mel:Metbo_1954"/>
    <dbReference type="eggNOG" id="arCOG00618">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="OrthoDB" id="89881at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="245" mass="26242" checksum="AA6930E7986E22F4" modified="2011-05-03" version="1">MILLYIIPAVDIKNGKCVQLVQGKPGTEQVIIDNPHEVAKNWEDKGAEILHVINLDGAFGENSKNREVIEKIIDTVSVPVQLGGGIRTVDDAANLLDLGVEKVILGTMAVENPELVNQLSDDYGSDSIIVALDSKNSEVVVKGWTESTGKTAPELGLSLQKHGAGGILFTNVDVEGLLSGFNMDPLLELLDAVNIPVIYSGGVTTLEDIETLSKTKTFGTVIGSALYKGRIDFETALKFQAVHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB35</accession>
    <name>F0TB35_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645532.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1961"/>
    <dbReference type="EnsemblBacteria" id="ADZ10181">
        <property type="protein sequence ID" value="ADZ10181"/>
        <property type="gene ID" value="Metbo_1961"/>
    </dbReference>
    <dbReference type="GeneID" id="10278421"/>
    <dbReference type="KEGG" id="mel:Metbo_1961"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="OMA" id="INDQWHR"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="39"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17451" checksum="A2D4F385A833FB33" modified="2011-05-03" version="1">MKRNDAEEVFNDIYTTLLEKQREIGDMVDSYITNTPKNTPSTIIQPAVDILDEDETVVVNVDLPGIEREKIKLDITERSIELTADYRDEREAEKNYLTRERYSEIIERKISLPEGLDINNTAAKFEGGVLSVTIPKIVETPMEENNFEVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB40</accession>
    <name>F0TB40_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NIL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645537.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1966"/>
    <dbReference type="EnsemblBacteria" id="ADZ10186">
        <property type="protein sequence ID" value="ADZ10186"/>
        <property type="gene ID" value="Metbo_1966"/>
    </dbReference>
    <dbReference type="GeneID" id="10278426"/>
    <dbReference type="KEGG" id="mel:Metbo_1966"/>
    <dbReference type="eggNOG" id="arCOG02460">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152999_0_0_2"/>
    <dbReference type="OMA" id="TGVCPTE"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14148" checksum="27E5C1057340C521" modified="2011-05-03" version="1">MKAWLYFSPKMANKAVITDLVKNFDVNFNILRADIDSQGGKMLMEINGPEAEQGIAYIENAGISVSPIKRVVKKDEEMCIDCGACVSLCPVHAIIVEDDWTVEVKDKECIGCKLCSYSCPTKAIKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB89</accession>
    <name>F0TB89_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein S14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0789"/>
    <dbReference type="EnsemblBacteria" id="ADZ09040">
        <property type="protein sequence ID" value="ADZ09040"/>
        <property type="gene ID" value="Metbo_0789"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0789"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_2_2_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="40S_S29/30S_S14z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="PTHR12010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="47" mass="5657" checksum="8FDBE3A300E0209F" modified="2011-05-03" version="1">MPRKYGKASRKCSRCNDHSALVRRYNLMLCRQCFRELADKIGFKKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB93</accession>
    <name>F0TB93_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl19e</name>
        <name evidence="9" type="ordered locus">Metbo_0793</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644395.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0793"/>
    <dbReference type="EnsemblBacteria" id="ADZ09044">
        <property type="protein sequence ID" value="ADZ09044"/>
        <property type="gene ID" value="Metbo_0793"/>
    </dbReference>
    <dbReference type="GeneID" id="10277241"/>
    <dbReference type="KEGG" id="mel:Metbo_0793"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="OrthoDB" id="109335at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_L19/L19e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_L19/L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_L19/L19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_L19/L19e_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_L19e_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="RPL19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="PTHR10722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="SSF48140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L19e" evidence="8">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="17000" checksum="929FFC9DC26594F0" modified="2011-05-03" version="1">MNLTTQKRLAADILKVGVNKVWIDPESVEEVSRAITRESVKQLIDSGAIKARQKKGISSYRSKKIAEQKSKGRRKGRGSIKGAKGARNPKKKAWMTTIRALRTDLKDMRDKREINKTTYRKLYRMAKGGAFRSKSYMKTYARDHDLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA3</accession>
    <name>F0TBA3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">50S ribosomal protein L14e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644405.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0803"/>
    <dbReference type="EnsemblBacteria" id="ADZ09054">
        <property type="protein sequence ID" value="ADZ09054"/>
        <property type="gene ID" value="Metbo_0803"/>
    </dbReference>
    <dbReference type="GeneID" id="10277251"/>
    <dbReference type="KEGG" id="mel:Metbo_0803"/>
    <dbReference type="eggNOG" id="arCOG04167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="OMA" id="RRCNIKH"/>
    <dbReference type="OrthoDB" id="118643at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="76" mass="8291" checksum="67AEDDC575C34113" modified="2011-05-03" version="1">MPAIEVGRICVKIAGREAGEKCVIVEIIDDNYVEVVGSTIKNRKCNLKHLEPVDQTIEVKSDDVEAIKKELAAAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB0</accession>
    <name>F0TBB0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl13</name>
        <name evidence="5" type="ordered locus">Metbo_0810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644412.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0810"/>
    <dbReference type="EnsemblBacteria" id="ADZ09061">
        <property type="protein sequence ID" value="ADZ09061"/>
        <property type="gene ID" value="Metbo_0810"/>
    </dbReference>
    <dbReference type="GeneID" id="10277259"/>
    <dbReference type="KEGG" id="mel:Metbo_0810"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OMA" id="GMLPWKT"/>
    <dbReference type="OrthoDB" id="87367at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_L13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="PTHR11545:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="15763" checksum="6635171C9FF5C241" modified="2011-05-03" version="1">MIIDGEGLVLGRLASKVSKLILAGESVDVLNAEKIIISGSKDWAYAKYKQRVDRASISNPRDMGPKYPRRPDDIFRRTVRGMIPYKQPKGRDAFKLLKVYVGVPKEFETQEKTRIVEAEPRNIVKSVKLGTVSELLGSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD7</accession>
    <name>F0TBD7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645557.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10206">
        <property type="protein sequence ID" value="ADZ10206"/>
        <property type="gene ID" value="Metbo_1986"/>
    </dbReference>
    <dbReference type="GeneID" id="10278446"/>
    <dbReference type="KEGG" id="mel:Metbo_1986"/>
    <dbReference type="HOGENOM" id="CLU_2420062_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="91" mass="11234" checksum="BB535A2DE3D43DCF" modified="2011-05-03" version="1">MPKRKPRLIILKQEISKFDNWFKRVLLREKSAIYAHKKNELNYKFDQYHRFLDALESYDMITVQDFNQSLNEINQKRIKEEEDLAKTIWQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE3</accession>
    <name>F0TBE3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1992"/>
    <dbReference type="EnsemblBacteria" id="ADZ10212">
        <property type="protein sequence ID" value="ADZ10212"/>
        <property type="gene ID" value="Metbo_1992"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1992"/>
    <dbReference type="HOGENOM" id="CLU_3075344_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="6145" checksum="AA76EE068623055E" modified="2011-05-03" version="1">MNFLMNFGKLVVSQIMSNVIHTFVLIIEHLIIERLINTLFNIFLIIIFLIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF3</accession>
    <name>F0TBF3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645573.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2003"/>
    <dbReference type="EnsemblBacteria" id="ADZ10222">
        <property type="protein sequence ID" value="ADZ10222"/>
        <property type="gene ID" value="Metbo_2003"/>
    </dbReference>
    <dbReference type="GeneID" id="10278463"/>
    <dbReference type="KEGG" id="mel:Metbo_2003"/>
    <dbReference type="eggNOG" id="arCOG02008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080422_2_0_2"/>
    <dbReference type="OMA" id="GGMERMI"/>
    <dbReference type="OrthoDB" id="88126at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="24087" checksum="281BCDCDE3262ABD" modified="2011-05-03" version="1">MKFQRGSIFRPEKDYYKYAGTVLIVATIQFFLAITLAETQFPGYSVSKNSLSYLGGSIPLVEPSAMIFNVSVILFGLLGILAVYLILKSGGCRLFSICLLISSLGAVGVGLFPEYTGSTHLFFALTTFLFGSLATIFSYRLGLNIPMVIISLVLGIGSLLTIILLLVLGSTPASNPLIAYLGVGGNERFIAYPIILYLTALGGYLTSRGKDWVRIRFTNGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG3</accession>
    <name>F0TBG3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
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                <person name="Brauer S.L."/>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
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                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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</entry>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
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    </reference>
    <reference evidence="7 8" key="2">
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            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="382" mass="41286" checksum="E963D0A179DCF64C" modified="2011-05-03" version="1" precursor="true">MNLSRTLAVTKRVFKDVKNDKRTIALILIAPIFAMFVFGLAFSGNVEDVSTIIVNHDNGFVSPSGQNTSISKDIISNLNTSVMNITYMTDDAAATKEVQDGKSYAVIIFPENFTQEIIQSQTGTNSGNSSSSTEITIKDDESIVNIKTAITGSVADAVTTTMENNGVNPPVKLSTDPIYGQNAQFIDFVVPGVMGFIVYLLTTLLTLLAFVGERTSGTLERLLSSPIRESEMVGGYAIAFGIIGTVQAAFLLLVAIAVFRITVIGDVFLAFLVVALLAVVSQALGILLSNLAKTEGQAVQFVPFIILPVFLLSGVFWPIQAIPVWLRPLSYLVPPTYAVDACRAVLLKGWGLSRIWPDILALILFAVLFLILATLSLKRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL6</accession>
    <name>F0TBL6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644444.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0843"/>
    <dbReference type="EnsemblBacteria" id="ADZ09093">
        <property type="protein sequence ID" value="ADZ09093"/>
        <property type="gene ID" value="Metbo_0843"/>
    </dbReference>
    <dbReference type="GeneID" id="10277292"/>
    <dbReference type="KEGG" id="mel:Metbo_0843"/>
    <dbReference type="eggNOG" id="arCOG13996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176004_0_0_2"/>
    <dbReference type="OMA" id="SASSCWH"/>
    <dbReference type="OrthoDB" id="131845at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8177" checksum="3773ADE7DBAF7086" modified="2011-05-03" version="1">MISTVTTTTTTVTTTEVMAYSIIAVIALIALLALKEILSSETENKTMKSFVTGSNIAIVPLLLVFVAIVAYKVVTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS2</accession>
    <name>F0TBS2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644500.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09149">
        <property type="protein sequence ID" value="ADZ09149"/>
        <property type="gene ID" value="Metbo_0900"/>
    </dbReference>
    <dbReference type="GeneID" id="10277349"/>
    <dbReference type="KEGG" id="mel:Metbo_0900"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782140_0_0_2"/>
    <dbReference type="OMA" id="RITHRTE"/>
    <dbReference type="OrthoDB" id="96512at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="354" mass="40771" checksum="AC9922BADB658495" modified="2011-05-03" version="1">MVNKKIFSISMVKNEMDIIESFVRYNVNYLDGMIILDNGSTDKTLEMLKSLKNEGLPIFVIEDDTRAFDKILKMNMLLNKAVHEYNADIVTPLDADEFMLSSDGGSPREHIEKLTSPNYFVAKWKVYVPDFTKNQEERFIPRKIVMARDDSTRDWHTLYKVIIPRELVTDYGVTVTRGSHSLVIPPEHKNNLKRVVNPDLRIAHFPIRSKEQTVSKVAVGWLNAISSVEKKPNDSFHWKNIFSQLKSQKEIADEDVVAIASEFSCENDDTPKLLTEDPMDLSFCTNIELKYTNEKINPMSNLLESCEYMAQSCVDAKKLSIKQQNKLNQMENSISWKITSPLRKAKAWAKRLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU7</accession>
    <name>F0TBU7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">leuC</name>
        <name evidence="8" type="ordered locus">Metbo_2070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645640.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2070"/>
    <dbReference type="EnsemblBacteria" id="ADZ10289">
        <property type="protein sequence ID" value="ADZ10289"/>
        <property type="gene ID" value="Metbo_2070"/>
    </dbReference>
    <dbReference type="GeneID" id="10278532"/>
    <dbReference type="KEGG" id="mel:Metbo_2070"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OMA" id="GIEHCLL"/>
    <dbReference type="OrthoDB" id="15714at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="414" mass="44638" checksum="F68E560D0F9232A6" modified="2011-05-03" version="1">MNITEKILARAAGKEEVHPGEIIEVDVDLAMAHDGTSPPTINNFKKIQDKVWDPEKIVIICDHTVPPNTIGSAEFHKVSREFARDQGIKNYLNHGEGICHQVLPEYGFVKPGEVVVGADSHTCTHGAFGAFATGVGATDMAFIFATGQTWFRVPEAYKITVDGKLGNFVSAKDVILNVIGEIGTYGATYKSLEFCGNTIENMDVAGRMTICNMAIESGAKNGIMEPNKATLQYLKDRNVKGFDVVTSDPDYTYEKEYFFDVDDMEPQVACPHNVDNVKPLSKVTGKTINQAFIGSCTNGRMEDLVAAAEVLEGKRVHPDVRLLIIPASAEIYRKAINSGLVDIFMDSGAIICNPGCGPCLGGHMGVLAAGEVCVATTNRNFLGRMGDPESEVYLANPAVVASSALSGEINDPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX6</accession>
    <name>F0TBX6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TOPRIM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645669.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2099"/>
    <dbReference type="EnsemblBacteria" id="ADZ10318">
        <property type="protein sequence ID" value="ADZ10318"/>
        <property type="gene ID" value="Metbo_2099"/>
    </dbReference>
    <dbReference type="GeneID" id="10278562"/>
    <dbReference type="KEGG" id="mel:Metbo_2099"/>
    <dbReference type="eggNOG" id="arCOG00383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965121_0_0_2"/>
    <dbReference type="OMA" id="EMVGHIE"/>
    <dbReference type="OrthoDB" id="72755at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="8"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="296" mass="34067" checksum="66F5A6BEED6DE185" modified="2011-05-03" version="1">MDAINPIDVRIIVEGASDVEIVSRAMQNIALGAEYHITISSIIPTTNPEIAKKAVQGADIVLIATDVDAPGRELAEKFRKCLKDKVGHVERMKFPFGHDVEYMDPSIIRKEIKNAIIRTGLISIANVQKFRELENNLTESRENILILAKEKKNLETENQNLVSVNEDLTNSNSKLTEKLRIVEDEFRIAKHKHADIKNKYQFIKSKQLFERFSLKDLWKELFDEELNDEEQVYFISNEFKPEKIVVGQGYIAATSKEAAKEWLNIIRAVLIFYDSKIEDLKDEFTDEKFNPNLLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC28</accession>
    <name>F0TC28_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0932</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644530.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0932"/>
    <dbReference type="EnsemblBacteria" id="ADZ09179">
        <property type="protein sequence ID" value="ADZ09179"/>
        <property type="gene ID" value="Metbo_0932"/>
    </dbReference>
    <dbReference type="GeneID" id="10277381"/>
    <dbReference type="KEGG" id="mel:Metbo_0932"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="OrthoDB" id="1231at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="4">
        <location>
            <begin position="388"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="4">
        <location>
            <begin position="490"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="592" mass="62670" checksum="5FB4BFA4AACFF417" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC50</accession>
    <name>F0TC50_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="11" type="ordered locus">Metbo_0954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644552.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0954"/>
    <dbReference type="EnsemblBacteria" id="ADZ09201">
        <property type="protein sequence ID" value="ADZ09201"/>
        <property type="gene ID" value="Metbo_0954"/>
    </dbReference>
    <dbReference type="GeneID" id="10277403"/>
    <dbReference type="KEGG" id="mel:Metbo_0954"/>
    <dbReference type="eggNOG" id="arCOG03055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_5_1_2"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="OrthoDB" id="14000at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="405"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="435" mass="46077" checksum="293CC7C9E52BD35A" modified="2011-05-03" version="1">MNFKEFIKDYRVILLIVLLIVSIGAISVFGIQEGLDLKGGSIIQIHLDQPVDTDTMSKVTNVLDKRLNTFGVTDVKVRASGNQDVVVEIANVAPDQVAKVVGTPGKFEAKIGNETVLTGTDITTVKAYEVTGNQWNVPFTVTSDGASKFAKAANGKAGTPVDMYLDDQIVSSPEISAELANGVPSTDVQVSGTSQTNQAAKDQAKSLQVVLQSGALPVKVSIVGVSSVSAELGSQFKTGALIAGGLALLVIAFIVFLRYRTPVLVIPIIFTSVAELIIILGVASIIKWNIDLAAIAGILAAIGTGVDDQIIITDEVLKKGVATKRTRTALKFKINNAFFIIFASAATLIAAMLPLAYVGFSRGATGIGLLSGFAFTTILGVLIGIFITRPVYAKFIELLLGKDRKEEKETKPSRSKSSEVGKKPKNKKKGKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC89</accession>
    <name>F0TC89_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645707.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2138"/>
    <dbReference type="EnsemblBacteria" id="ADZ10356">
        <property type="protein sequence ID" value="ADZ10356"/>
        <property type="gene ID" value="Metbo_2138"/>
    </dbReference>
    <dbReference type="GeneID" id="10278601"/>
    <dbReference type="KEGG" id="mel:Metbo_2138"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136066_0_0_2"/>
    <dbReference type="OMA" id="MFRTIRV"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="190"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="28740" checksum="C581725B10A39286" modified="2011-05-03" version="1">MVKREPLKDMQIELFATSEGIYAINSPVRVKILSMLRKGELSFDQLVSLSEKAKSTVSVHLRRMVNEGIIGSKTDPQDARKKIFFIKSEYLGKLSGKKDVTGNIEEFVSSYVDSEGDPYEFFRLIFHTIRVSLIRQGIDIDPILYEAGVKVGETLYNKVKDPDINKLAENIAMFWETHNLGHVEVKQLDPLIIDVSDCFECQSLPPIGRPACAFDSGILKAVFSAHFQEEFVVNETKCYTMGDNQCSFTIKNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA9</accession>
    <name>F0TCA9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2161"/>
    <dbReference type="EnsemblBacteria" id="ADZ10376">
        <property type="protein sequence ID" value="ADZ10376"/>
        <property type="gene ID" value="Metbo_2161"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2161"/>
    <dbReference type="HOGENOM" id="CLU_1943869_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="129" mass="15102" checksum="BE595807024C6C0E" modified="2011-05-03" version="1">MMNKDTIPGYRNSKDWNKGFKLFMDYFRDSLKFIRCGGDGSKYHSDGETLEEMNQNFSKVVDKLVNGAMALQYISDKDFYYKNEDVEIIIKMSDQSNDINLSKLLEKVFNILKSEEKQFVRIVNALETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF9</accession>
    <name>F0TCF9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrogenase maturation protease HycI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2212"/>
    <dbReference type="MEROPS" id="A31.003"/>
    <dbReference type="EnsemblBacteria" id="ADZ10426">
        <property type="protein sequence ID" value="ADZ10426"/>
        <property type="gene ID" value="Metbo_2212"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2212"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_4_1_2"/>
    <dbReference type="OMA" id="EMCAANP"/>
    <dbReference type="OrthoDB" id="124075at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06067">
        <property type="entry name" value="H2MP_MemB-H2evol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004420">
        <property type="entry name" value="Pept_A31_hyd_mat_HycI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00142">
        <property type="entry name" value="hycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="18947" checksum="FE89550C33783E96" modified="2011-05-03" version="1">MLEQELKKFIQNHENIIFLCIGNEMRGDDAAGPIIAEKFGQEIQKHPDKYLMMSVVNAGTVPENYTGMIRSKQPSHIIFVDAVEMGLDPGSLRLVHQDEIASYSISTHAMPLSFMIKYLKSFSDAEIILIGIQPKSLEMFEEISEELKEGIESLMIELIRIFQCQDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL2</accession>
    <name>F0TCL2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644640.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09289">
        <property type="protein sequence ID" value="ADZ09289"/>
        <property type="gene ID" value="Metbo_1042"/>
    </dbReference>
    <dbReference type="GeneID" id="10277491"/>
    <dbReference type="KEGG" id="mel:Metbo_1042"/>
    <dbReference type="HOGENOM" id="CLU_2433882_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="10418" checksum="259E4ED314FA8A06" modified="2011-05-03" version="1">MEPEPISGIVKGCKVICQPKELKEYEEGVKVVVVSEKLFSKLMSSLSEMEEDLMRLNDLHLDDTIKNIEKVRLKIENNLKELSYSEYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL5</accession>
    <name>F0TCL5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644643.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1045"/>
    <dbReference type="EnsemblBacteria" id="ADZ09292">
        <property type="protein sequence ID" value="ADZ09292"/>
        <property type="gene ID" value="Metbo_1045"/>
    </dbReference>
    <dbReference type="GeneID" id="10277494"/>
    <dbReference type="KEGG" id="mel:Metbo_1045"/>
    <dbReference type="HOGENOM" id="CLU_049046_5_0_2"/>
    <dbReference type="OMA" id="IVTIKLH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039981">
        <property type="entry name" value="AHSA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="PTHR13009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14871" checksum="46DF57F8C6A810A8" modified="2011-05-03" version="1">MRETITKIYKIRSSIREVWKALVDQSVIDKWGGGPSKMTETVGTDFELWNGDIYGRNIKVEHESQLVQEWFGGDWAKPSIVTFNLKEQYPYVILELEQINVPDPDVEDIDAGWDDFFLGPMKKMLETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL7</accession>
    <name>F0TCL7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Capsule synthesis protein, CapA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1047"/>
    <dbReference type="EnsemblBacteria" id="ADZ09294">
        <property type="protein sequence ID" value="ADZ09294"/>
        <property type="gene ID" value="Metbo_1047"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1047"/>
    <dbReference type="eggNOG" id="arCOG07503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038823_2_2_2"/>
    <dbReference type="OMA" id="LYGCGDF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07381">
        <property type="entry name" value="MPP_CapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019079">
        <property type="entry name" value="Capsule_synth_CapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09587">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00854">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGA_cap" evidence="1">
        <location>
            <begin position="42"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="38426" checksum="D08BF33018D985A5" modified="2011-05-03" version="1">MKKVAILILVVVVLLVAYGVNSLFNSSSNNSMSTSVSKSNITMYFTGDVMLGRNVESVLASGQNVFANVDEMFKNSDGAVINLEDPMTTSSTPYKSTIPLKANPAYAHVLKDNNIIVACLANNHIMDYGDTGLNDTISALKASGINYTGAGNNVDQSSKPVYLNIKDRKMAILNYMDNTTFTGFSASEMAAATSSSAGYAPADPNLIKKDIDEAKKNSDMVIVVFHYGNEYSTQPNTYQVTLSHACIDDGADMVIGSHPHVIQTIETYNGKPIFYSLGNFVFDMSNSATHESLMVEMDLNNNTANIKVHPMVLVNYMPQLMNNASSTQLLENLKNESPVNMTITGGEGVISFPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM2</accession>
    <name>F0TCM2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09299">
        <property type="protein sequence ID" value="ADZ09299"/>
        <property type="gene ID" value="Metbo_1052"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1052"/>
    <dbReference type="HOGENOM" id="CLU_3003155_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="56" mass="6407" checksum="C3C4DA8E5D8D67DA" modified="2011-05-03" version="1">MADEKCDVDLSKKKKISKDNKFHDGTNPELLLGPKGLDSERIKKKNKVDNLKRDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR8</accession>
    <name>F0TCR8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphosulfolactate synthase</fullName>
            <ecNumber evidence="2">4.4.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.19" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645809.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2244"/>
    <dbReference type="EnsemblBacteria" id="ADZ10458">
        <property type="protein sequence ID" value="ADZ10458"/>
        <property type="gene ID" value="Metbo_2244"/>
    </dbReference>
    <dbReference type="GeneID" id="10278710"/>
    <dbReference type="KEGG" id="mel:Metbo_2244"/>
    <dbReference type="eggNOG" id="arCOG04896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062679_2_0_2"/>
    <dbReference type="OMA" id="MFEASNA"/>
    <dbReference type="OrthoDB" id="37705at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043817">
        <property type="term" value="F:phosphosulfolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022370">
        <property type="entry name" value="Arch_ComA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="SSF102110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03849">
        <property type="entry name" value="arch_ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="28446" checksum="CE51571A5DF287B7" modified="2011-05-03" version="1">MNAFDFLTKDRIRPSGEGVTMMLDKGMGPNMVEDFLEISGNYVDLAKFGWGTSAVHERELIKYKIDTYISNNVVPYPGGTLFEQAYITNKFNEFLKEAQELGFGAIEISDGTVDISAVERSDIIGMVKDAGFMVLTEVGKKDPAKDHLLSPGDRVKLVEQDLESGADKVIIEAREGGKDLGIYNGKGEVKEEDMQILIDALDVNQLIWEAPQKNQQTYMILKFGPNVNLGNIAPEEVTALETMRRGLRGDTLGKVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS1</accession>
    <name>F0TCS1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="10" type="ordered locus">Metbo_2247</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645812.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2247"/>
    <dbReference type="EnsemblBacteria" id="ADZ10461">
        <property type="protein sequence ID" value="ADZ10461"/>
        <property type="gene ID" value="Metbo_2247"/>
    </dbReference>
    <dbReference type="GeneID" id="10278713"/>
    <dbReference type="KEGG" id="mel:Metbo_2247"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="OrthoDB" id="41117at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 6 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="8">
        <location>
            <begin position="45"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="9">
        <location>
            <begin position="238"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="53"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="382" mass="40772" checksum="34F059DB905BB907" modified="2011-05-03" version="1">MKSLINNTIKESEKRKERSTMEERNSFDDNIDSDLKEIIQRSRAKIFVVGTGGAGNNTVSRLAEIGVEGAGTLSVNTDAQDLFYSKSDHKILIGRSTCGGLGAGGIPDIGEESAEESEEQIKEKLEGADMVFVTCGLGGGTGTGSAPVISKLAKKIGALTIAVATMPFSAEGLRRRENAEKGLEKLQDAADTVIVIPNDKLLEVAPNLPINKAFMVADELLGRAVKGITELITKPGLVSLDFADIRSIMMGSGMAMIGMGESDSGDRAIESVHEALNSPLLDLDISNAKGALINICGSSDLTLHEAEKVVQIVADELDPDANIIWGTQIQEDLENVIRTTIVVAGVKSPHIFGMPGEKEYVEEKQRESVPESSLEEFIDGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV7</accession>
    <name>F0TCV7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2283</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2283"/>
    <dbReference type="EnsemblBacteria" id="ADZ10497">
        <property type="protein sequence ID" value="ADZ10497"/>
        <property type="gene ID" value="Metbo_2283"/>
    </dbReference>
    <dbReference type="GeneID" id="10278749"/>
    <dbReference type="KEGG" id="mel:Metbo_2283"/>
    <dbReference type="eggNOG" id="arCOG04921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_1_1_2"/>
    <dbReference type="OMA" id="EWQEFVN"/>
    <dbReference type="OrthoDB" id="72293at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="290" mass="33965" checksum="F3BE6E9B6F329D17" modified="2011-05-03" version="1">MTVPKVIAHQEKTAQILNKIFILIQNKTGHDFSAYKKSTINRRIQKRMKTCQVDEICDYLEYLHENPDEIHLLYNELLINVTQFFRDPEAYESLKSKFFINILEAKDDDVIRIWVPGCSSGEEVYSIAIMISELFEEMNQNFKVQIFGTDIDESVIRKARSGKYKISSNIHYDRLCKYFYKNGDEYTIKNEIREMMIFSTHNVITDPPFTRLDMISCRNLLIYLESSAQQQVISNFVNALNKDGILFLGPSENIGKFLDTFKIEDNKWNIFRGANSGRMRLNLKTNFLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S6</accession>
    <name>F0T5S6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644670.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09319">
        <property type="protein sequence ID" value="ADZ09319"/>
        <property type="gene ID" value="Metbo_1074"/>
    </dbReference>
    <dbReference type="GeneID" id="24964529"/>
    <dbReference type="KEGG" id="mel:Metbo_1074"/>
    <dbReference type="eggNOG" id="arCOG06789">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088478_1_0_2"/>
    <dbReference type="OMA" id="LRANTIW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30748" checksum="FE8A1FBE24EB72E5" modified="2011-05-03" version="1">MKTEVSAGTYRLKPTLLNALLIVVVYAAILVVIEMFMGVPYTEFSKSTNNMFMGVLIPIAMGSVILTSIALWSGWWNELWRDKYHIKDNGWMHIFLVLFIVAISMNFLVGHISSLRPTYILVTLMATILVGYSEELLTRGLLVCGARGSGYSEVKVFYIVMIVFGFIHGINFINGQSLGLTIQQMFMAGLLGGVFYTIFRKTGFLIVPMIVHALWDFSLFTQGFNFVGELITTSTGTYNPLQIIGIMAIYSTYLLLILAVRNFNVEKSELISLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W8</accession>
    <name>F0T5W8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type I site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="2">3.1.21.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.21.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1116"/>
    <dbReference type="REBASE" id="33845">
        <property type="entry name" value="Msp21ORF1113P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09361">
        <property type="protein sequence ID" value="ADZ09361"/>
        <property type="gene ID" value="Metbo_1116"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1116"/>
    <dbReference type="eggNOG" id="arCOG00880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009326_0_0_2"/>
    <dbReference type="OMA" id="NNFEYWN"/>
    <dbReference type="OrthoDB" id="2473at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013670">
        <property type="entry name" value="EcoEI_R_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08463">
        <property type="entry name" value="EcoEI_R_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="182"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="911" mass="106013" checksum="7788C9E05FBF2AFB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T619</accession>
    <name>F0T619_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645877.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10526">
        <property type="protein sequence ID" value="ADZ10526"/>
        <property type="gene ID" value="Metbo_2312"/>
    </dbReference>
    <dbReference type="GeneID" id="10278778"/>
    <dbReference type="KEGG" id="mel:Metbo_2312"/>
    <dbReference type="HOGENOM" id="CLU_2379423_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10712" checksum="68C5462B53D12A11" modified="2011-05-03" version="1">MPIQSNAQQTYSGYIEDSPNTITYYKDDKTTVSVHSLENCYWNESDPVHDIWSFTSITNVLTKSLPAFDDSTSFITQINDVLEVAYDYIISVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T658</accession>
    <name>F0T658_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2352"/>
    <dbReference type="EnsemblBacteria" id="ADZ10565">
        <property type="protein sequence ID" value="ADZ10565"/>
        <property type="gene ID" value="Metbo_2352"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2352"/>
    <dbReference type="eggNOG" id="arCOG04458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110565_0_0_2"/>
    <dbReference type="OMA" id="TVHATRY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="205" mass="22940" checksum="C4B6093B89D6DADE" modified="2011-05-03" version="1">MDLKDIGMERGLLYETVVTTVDSNGKPNAAPIGIICKNNLEVVCYLHHGSTTVKNIKDTKSFVVNVLKDPMIFVESTLGNLPIEKYESHGDKFYIGSTDAFFVADVTSIKDVERKDQFGVSVTSVLKADVSDITKFKQCVEPLNRAIYGIVEGLVYLTRMNMVSGDMEKLYRHRMAEIARIVSKVGAKDHKNAMDMIMEAFKDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T660</accession>
    <name>F0T660_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2354"/>
    <dbReference type="EnsemblBacteria" id="ADZ10567">
        <property type="protein sequence ID" value="ADZ10567"/>
        <property type="gene ID" value="Metbo_2354"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2354"/>
    <dbReference type="eggNOG" id="arCOG14641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_213598_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5691" checksum="9994A1972D70BBA7" modified="2011-05-03" version="1">MELQDLIYIVVALFLALILFYIFIWLVPIIIVLIIAYVIYIFLKGPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T667</accession>
    <name>F0T667_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645925.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2361"/>
    <dbReference type="EnsemblBacteria" id="ADZ10574">
        <property type="protein sequence ID" value="ADZ10574"/>
        <property type="gene ID" value="Metbo_2361"/>
    </dbReference>
    <dbReference type="GeneID" id="10278827"/>
    <dbReference type="KEGG" id="mel:Metbo_2361"/>
    <dbReference type="eggNOG" id="arCOG00116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893144_0_0_2"/>
    <dbReference type="OMA" id="SPKIINC"/>
    <dbReference type="OrthoDB" id="85237at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="35669" checksum="B3270EB54FDF3602" modified="2011-05-03" version="1">MELFYRRPSAKTRNAMCTAAQNLKHTPGKDEEISLAEDAIGKLTQHENVHVLNSGNSAIMAVMSCFKNGVMIPDQGGWYGFRKMAEFFGIETVKVPTNLGLIDLENLDQNIKQNNPEALFLTSFAGYTAEQPLKEIYEICEDNGIMLVEDASGGIGDPQGRLGNGNHAHVIVGSTGSPKTVNVGNGGFLSTNQPEISDNANYLLKILRADPVTCAGIASESEKAGEIFEKTSAATKRLKNNTSNFREVIHPTSRGLNILLPDRNQKLLGSEIRQNLNVHGGSIVSLSPNYNRIKARAVCIELKNLDVKCLTSKNLDEITDIIKLAGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T681</accession>
    <name>F0T681_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644747.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1152"/>
    <dbReference type="EnsemblBacteria" id="ADZ09396">
        <property type="protein sequence ID" value="ADZ09396"/>
        <property type="gene ID" value="Metbo_1152"/>
    </dbReference>
    <dbReference type="GeneID" id="10277602"/>
    <dbReference type="KEGG" id="mel:Metbo_1152"/>
    <dbReference type="eggNOG" id="arCOG07613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985567_0_0_2"/>
    <dbReference type="OMA" id="WILLANN"/>
    <dbReference type="OrthoDB" id="51989at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="32797" checksum="172B3CABDF0C025E" modified="2011-05-03" version="1">MFDILSEDSRGFAISMDLLLALIPLTLVLGFVAADMDNILYQMEDTVFRGATDRAASDAVSTLVTTSGDPVNWETTGVANVVGLAQYDTSKGAPVEGTIDPVKLAAVTDSQIQSIIGPNYHYYMNITTLNQTNTPVTIKLMGNASYPNAKDVVKVERVVLSSKFKVVTSLIQQIRFTGGGKTYTTSPFQTSYTSTNSYDYWILLANNQGFTAATVSVNNNVFSFNSANMGTPQKINSTFLTTNSTIPNQYFNNTVTFNGTGSFPSSIDLYIVQTPKNTGASEITSDTVKLKPSTFDLYLWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T695</accession>
    <name>F0T695_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644761.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1166"/>
    <dbReference type="EnsemblBacteria" id="ADZ09410">
        <property type="protein sequence ID" value="ADZ09410"/>
        <property type="gene ID" value="Metbo_1166"/>
    </dbReference>
    <dbReference type="GeneID" id="10277616"/>
    <dbReference type="KEGG" id="mel:Metbo_1166"/>
    <dbReference type="eggNOG" id="arCOG05786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929427_0_0_2"/>
    <dbReference type="OMA" id="YRDFFLM"/>
    <dbReference type="OrthoDB" id="84045at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018663">
        <property type="entry name" value="DUF2101_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09874">
        <property type="entry name" value="DUF2101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="303" mass="34671" checksum="EF5E61A9DB682CAA" modified="2011-05-03" version="1">MNIFSIIGHYVIKLFSLIGTVIMTIPKIPRLIRNFDSSEIKQHLDSENIKENISKIREETAIDERISKIREETAIDERISKIRGSETFKHYESSDDDSGFIMIGGAFSAEEKERTVLILQILSALFIVLSILSIFNFISAIIYIPLGIVVVGYIVYLIYNKIKLMYKSDFNAYRDFFLMYIAVGVILYVINFNTNLVMSFSFEVLPSLTVLIFALVAVIAVFLIFRVRYYRNFTYGTVLDSGKNTAHVKVDYDIRSNVKPDIYIVENHIGAVEGEQVKLQLEEKLMSMSGNRPVNIIETANRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B5</accession>
    <name>F0T6B5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644781.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09430">
        <property type="protein sequence ID" value="ADZ09430"/>
        <property type="gene ID" value="Metbo_1186"/>
    </dbReference>
    <dbReference type="GeneID" id="10277636"/>
    <dbReference type="KEGG" id="mel:Metbo_1186"/>
    <dbReference type="eggNOG" id="arCOG03730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773199_0_0_2"/>
    <dbReference type="OMA" id="LIHYRER"/>
    <dbReference type="OrthoDB" id="129930at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="17400" checksum="D7C0AD9AD8100857" modified="2011-05-03" version="1">MVSINDDDSKKSKKDYDAIIEELEIELAKKQVTIQNLKEGIQDANNRVSDMVEKNSSLESRINEYELQDLAMKFGKFEKLKDDNKKLDHRVKVTKKHLDSAREYLKFTYVVILDMKNRSIFDRVFRRYPDSYHRYVNDLNQESDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E3</accession>
    <name>F0T6E3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644809.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1215"/>
    <dbReference type="EnsemblBacteria" id="ADZ09458">
        <property type="protein sequence ID" value="ADZ09458"/>
        <property type="gene ID" value="Metbo_1215"/>
    </dbReference>
    <dbReference type="GeneID" id="24964540"/>
    <dbReference type="KEGG" id="mel:Metbo_1215"/>
    <dbReference type="eggNOG" id="arCOG01426">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_840950_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="16"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="38878" checksum="74B89491F93C45BE" modified="2011-05-03" version="1">MIKELIKKQIKKTGTYREVEIETQKLEKQIQTLDQVRQTQITALEKQLETSKNDKNKLNNQIETLQKDKNKLNNQIETLQKDKNKLNNQIETLQKDKNKLNNQIETLQKDKNKLNNQMQTLQKDKQALKKERPRLLGYSMKSRPRGVSADVGKPVILKYILENIKKNQKVLDVGFGSGVYGKLLRTFYYRYVDGIDVFDKHLEEFGLDLIYDNIYIENILEFEFEFYDLIIMGDVLEHIELEAAKKLLTKFIEEKKCDHLIVSIPFESEQGDVHENVHEIHLQDNVTAKYMEKHYPYLDLIDLYYNSELGNIKAVYIWNKDLKTNPTSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M7</accession>
    <name>F0T6M7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0007"/>
    <dbReference type="EnsemblBacteria" id="ADZ08260">
        <property type="protein sequence ID" value="ADZ08260"/>
        <property type="gene ID" value="Metbo_0007"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0007"/>
    <dbReference type="HOGENOM" id="CLU_1933265_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="13219" checksum="94615EC2F2CF34A2" modified="2011-05-03" version="1">MIGDGLVEFKEFGCGLFLAIVMVVLLGLLIGTYGTYLGMGIAGFVTGLISGSEIMDGLFNGIFVGMGTGLILSIFALILAMIYGVTAGLGAGLLAGNIGYGAEIYLIVVYIILASVGSLGGSLTLKLIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q0</accession>
    <name>F0T6Q0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkylhydroperoxidase like protein, AhpD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0030"/>
    <dbReference type="EnsemblBacteria" id="ADZ08283">
        <property type="protein sequence ID" value="ADZ08283"/>
        <property type="gene ID" value="Metbo_0030"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0030"/>
    <dbReference type="eggNOG" id="arCOG06151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115251_0_0_2"/>
    <dbReference type="OMA" id="FMERIML"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CMD" evidence="2">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="23604" checksum="B0460930B68E7C03" modified="2011-05-03" version="1">MNIKEKHKIRNIEPEFGKNLYSVRESYWIFYKGIRTMKYMFEAKRKKELSPKFIERIMLAVTEVNGCEICSYAHTKRALESGMSNEEIKNMLSGIIDDVPTNEVAAVLFAQHYADTRGNPTSESWQRIVDIYGISKANGILGSIRSIMIGNAYGIAWSSFFNRLRSRPDQRSSLLYEVSMMLGIILTPISIIHALISDLFRIPIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S6</accession>
    <name>F0T6S6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643660.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0056"/>
    <dbReference type="EnsemblBacteria" id="ADZ08309">
        <property type="protein sequence ID" value="ADZ08309"/>
        <property type="gene ID" value="Metbo_0056"/>
    </dbReference>
    <dbReference type="GeneID" id="10276479"/>
    <dbReference type="KEGG" id="mel:Metbo_0056"/>
    <dbReference type="eggNOG" id="arCOG09452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119491_2_0_2"/>
    <dbReference type="OMA" id="FYNFVMH"/>
    <dbReference type="OrthoDB" id="85171at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="18456" checksum="B061DFDB9D4A8E8A" modified="2011-05-03" version="1" precursor="true">MEFILILIILTILVALTTLIRNKIKNRSLNGLIIIKTFLLSFLVIFVGIGSIWAFIGHAFMPVLVAESIGWATGSPFQTEVAFANLAIGILGILCYFFRDNFWVATVIASSIFLLGAAYVHLINIISYSNHSVGNVGTIFFMDIIGPVILVVLLILYKLYERKQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U1</accession>
    <name>F0T6U1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional enzyme Fae/Hps</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="3">5,6,7,8-tetrahydromethanopterin hydro-lyase</fullName>
                <ecNumber evidence="3">4.2.1.147</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">Formaldehyde-activating enzyme</fullName>
                <shortName evidence="3">Fae</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">3-hexulose-6-phosphate synthase</fullName>
                <shortName evidence="3">HPS</shortName>
                <ecNumber evidence="3">4.1.2.43</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">D-arabino-3-hexulose-6-phosphate formaldehyde lyase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">fae-hps</name>
        <name evidence="5" type="ordered locus">Metbo_0071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10-methylenetetrahydromethanopterin.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:24678"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="EC" id="4.2.1.147"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25201"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58542"/>
            <dbReference type="EC" id="4.1.2.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the formaldehyde-activating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.147" evidence="3"/>
    <dbReference type="EC" id="4.1.2.43" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_0071"/>
    <dbReference type="EnsemblBacteria" id="ADZ08324">
        <property type="protein sequence ID" value="ADZ08324"/>
        <property type="gene ID" value="Metbo_0071"/>
    </dbReference>
    <dbReference type="GeneID" id="10276494"/>
    <dbReference type="KEGG" id="mel:Metbo_0071"/>
    <dbReference type="eggNOG" id="arCOG00103">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:hexulose-6-phosphate synthase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01268">
        <property type="entry name" value="Fae_Hps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020868">
        <property type="entry name" value="Fae/Hps"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014826">
        <property type="entry name" value="HCHO-activating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037075">
        <property type="entry name" value="HCHO-activating_enzyme_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08714">
        <property type="entry name" value="Fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03126">
        <property type="entry name" value="one_C_fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="4">
        <location>
            <begin position="174"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Formaldehyde-activating enzyme" evidence="3">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-hexulose-6-phosphate synthase" evidence="3">
        <location>
            <begin position="162"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="3">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="44724" checksum="C4DDB605DFA1F1C1" modified="2011-05-03" version="1">MYQIGEALIGNGNEIAHVDLVIGDKNGPVGTAFVNNMASMSIGHTPLLSVIRPNLLTKPATLIVPKVTVRDLEDADKIFGPAQTAVGRAVADAVDEGIIPKEDVEDIVIIANVFIHPGAKDFRKIYQYNYGATKLAIQRAMEGYPSVKKVLAEKDRGTHPIMGFKVTRLWNPPYLQVALDLDNMEEMERIIKTLPDRERILIEAGTPLVKKFGVGIIGKIRELKKDAFIIADLKTLDVGRIEVKMAADETADAIAISGLGTVESIEKAIHEAKKQGIYSILDMMNVENFVEKLEAVQFKPDIVLLHRNVDLETMQAERGEELAEVTEWGNIHQIKSILGEGKLVAVAGGITPDRVDKAIDSDADIIVVGRYIIGSRDVRRSAQDFLDRMPQDPDTMRLAMDEDESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W8</accession>
    <name>F0T6W8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644839.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1246"/>
    <dbReference type="EnsemblBacteria" id="ADZ09488">
        <property type="protein sequence ID" value="ADZ09488"/>
        <property type="gene ID" value="Metbo_1246"/>
    </dbReference>
    <dbReference type="GeneID" id="10277697"/>
    <dbReference type="KEGG" id="mel:Metbo_1246"/>
    <dbReference type="eggNOG" id="arCOG02734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_1_2"/>
    <dbReference type="OMA" id="GGCMAPV"/>
    <dbReference type="OrthoDB" id="120742at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="15"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="13275" checksum="C48CC8F5340EC1A2" modified="2011-05-03" version="1">MIICVPSMESTGLLSKISTHFGKTPYFTILDMENNEIKTLETKKIESRHAGGKKTPAEIIIEINPDVVLCANLGLKAVEMLKEHGIKIYVEASGTVENSLKAYLNNELKLADENTACSEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z2</accession>
    <name>F0T6Z2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644863.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1270"/>
    <dbReference type="EnsemblBacteria" id="ADZ09512">
        <property type="protein sequence ID" value="ADZ09512"/>
        <property type="gene ID" value="Metbo_1270"/>
    </dbReference>
    <dbReference type="GeneID" id="10277721"/>
    <dbReference type="KEGG" id="mel:Metbo_1270"/>
    <dbReference type="eggNOG" id="arCOG02179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097041_0_0_2"/>
    <dbReference type="OMA" id="CVKCEIC"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="155"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="192"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="27527" checksum="DCEDA0E8236B7BDE" modified="2011-05-03" version="1">MDITFKKRKDALVGDVVAKSNDLEHGVEDFKAEIENCSLEQKFITISPECVRCNLCAQECPVNAIDEAKLTKPAKILDNCVKCEICSQTCPVNAIKVIESKSDLEEDVTYKLKTKKVPHRTLRMEKIDVDPVKCTSCGNCVKFCPTGAIKLFDEGPAVVDKDSCIGCGACANVCGENAVSLERRLGSVFKTKKLAIDEDICVACGMCEENCPVEAIKLENGKIVYSKNKCITCEVCSKKCPVAALNVEEVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T704</accession>
    <name>F0T704_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Energy-converting hydrogenase A subunit I EhaI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644875.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1282"/>
    <dbReference type="EnsemblBacteria" id="ADZ09524">
        <property type="protein sequence ID" value="ADZ09524"/>
        <property type="gene ID" value="Metbo_1282"/>
    </dbReference>
    <dbReference type="GeneID" id="10277733"/>
    <dbReference type="KEGG" id="mel:Metbo_1282"/>
    <dbReference type="eggNOG" id="arCOG06464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730476_0_0_2"/>
    <dbReference type="OMA" id="ADWIVYA"/>
    <dbReference type="OrthoDB" id="124359at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="7982" checksum="996D6593D5CD3716" modified="2011-05-03" version="1" precursor="true">MDNLKTTGWILLIASFASIVYAIIFNPTSWIVYAISLVSIPLFILSLGLVTMAKGSKDEEEEKRREPFIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T716</accession>
    <name>F0T716_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.47" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644887.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1294"/>
    <dbReference type="EnsemblBacteria" id="ADZ09536">
        <property type="protein sequence ID" value="ADZ09536"/>
        <property type="gene ID" value="Metbo_1294"/>
    </dbReference>
    <dbReference type="GeneID" id="10277745"/>
    <dbReference type="KEGG" id="mel:Metbo_1294"/>
    <dbReference type="eggNOG" id="arCOG01373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="FVKSSWQ"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019673">
        <property type="term" value="P:GDP-mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="PTHR43715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="409" mass="45590" checksum="C5A5492A88C88313" modified="2011-05-03" version="1">MNWNDKNIFITGVGGFAGSYLAEELLNQGANVFGLVRRRADGTKPKNLIDHGIQNDVTALEGDLTNITSLANALDESEPDYIFHLAAQSFVPRSFENSAETQIINCIGTSNLLEASRVKDTDAKIVFAGSSEEYGLVISSENQYESATKEYGTIFPKPETIPELPIKETNPLRPMSPYAVSKVYGDHLMQNYYHSYGLDTTVSRAFNHEGAGRGLMFVTSVVTNQIMKLKFGEIENITIGNLNAFRDWSHVKDIVQGYMTLATKGKSGEVYNQGSMRTNSVLSYILLGLEEAGWNIESAETFKGEKKISNPTESDNSSVYGVNFEKTVVDRMLLEGELEYTLSDQGINLETDKGQVKIQFNPDRFRPAEVPILFSNTEKIQKIGATIQHSLKDIVKDQLNFYLKKDNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T730</accession>
    <name>F0T730_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-oxoacid:acceptor oxidoreductase, alpha subunit</fullName>
            <ecNumber evidence="5">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2451"/>
    <dbReference type="EnsemblBacteria" id="ADZ10664">
        <property type="protein sequence ID" value="ADZ10664"/>
        <property type="gene ID" value="Metbo_2451"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2451"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OMA" id="LNPFPKN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="3">
        <location>
            <begin position="18"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="POR_N" evidence="4">
        <location>
            <begin position="213"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="571" mass="62977" checksum="B4FDAA360321BD95" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T784</accession>
    <name>F0T784_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit E region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2505"/>
    <dbReference type="EnsemblBacteria" id="ADZ10718">
        <property type="protein sequence ID" value="ADZ10718"/>
        <property type="gene ID" value="Metbo_2505"/>
    </dbReference>
    <dbReference type="GeneID" id="10278979"/>
    <dbReference type="KEGG" id="mel:Metbo_2505"/>
    <dbReference type="eggNOG" id="arCOG00763">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112307_0_0_2"/>
    <dbReference type="OrthoDB" id="62422at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
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    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FmdE" evidence="1">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="22833" checksum="6BF564E74E774BF3" modified="2011-05-03" version="1">MEDVLNKIDNPSMKEDIKKLIKFHGYLSSGALIGYQMLNIAKRELDIHDDEKIYVTCETLNCVPDPFQILRSSTIGNKGLKVLDYDKMAVTVNKGAGKDEKFVKGIRIYFNAKKTEKYPILHDWYMNYKKVRHEEVVPILLEAGEDVYSWEFVEIEVPEKKKKNVLLCEKCGESFVSWDKSTICIPCSKDENNNTANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T785</accession>
    <name>F0T785_METLA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="877455.Metbo_2506"/>
    <dbReference type="EnsemblBacteria" id="ADZ10719">
        <property type="protein sequence ID" value="ADZ10719"/>
        <property type="gene ID" value="Metbo_2506"/>
    </dbReference>
    <dbReference type="GeneID" id="10278980"/>
    <dbReference type="KEGG" id="mel:Metbo_2506"/>
    <dbReference type="eggNOG" id="arCOG05038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340162_0_0_2"/>
    <dbReference type="OMA" id="TIERCET"/>
    <dbReference type="OrthoDB" id="109265at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11863" checksum="0EB0AA9AE0D1D694" modified="2011-05-03" version="1">MEKILKMDCDEAISYVKDNVNDYDYLELSYNRVFTPGEVISVETDHLKGKDVCNVMIQVKGDTVRNTILIDLEEVKDDLIEMRHVPKDKEEGILIEIERCEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H3</accession>
    <name>F0T7H3_METLA</name>
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        <submittedName>
            <fullName evidence="3">Peptidase T-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644892.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1299"/>
    <dbReference type="EnsemblBacteria" id="ADZ09541">
        <property type="protein sequence ID" value="ADZ09541"/>
        <property type="gene ID" value="Metbo_1299"/>
    </dbReference>
    <dbReference type="GeneID" id="10277750"/>
    <dbReference type="KEGG" id="mel:Metbo_1299"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_6_0_2"/>
    <dbReference type="OMA" id="GHNFHGK"/>
    <dbReference type="OrthoDB" id="14646at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010162">
        <property type="entry name" value="PepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42994:SF2">
        <property type="entry name" value="PTHR42994:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01883">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="178"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="42229" checksum="DF8A5CF12F6A726C" modified="2011-05-03" version="1">MNTKRLINTFLDLISIYSPSLQERYVCDYLTRRLKDLGFEVYEDLVGEQIGGNSGNLYGYLEGDESLEPLLFCAHMDTVEPARGKKVIMHDDGMITSDGKTILGSDAMSGVSAILEAISTLKEDNNNHRPIEVLFCVAEEIYGLGSKFFDYSAIKSKQSYTLDLSGDVGSAAFKAPSLLTFDMIVEGKSGHSNFKSDNTINAIIVACKAVSQLKTGKIDTRSTLNIGLIQGGRAPNIIPDRCAVKGEIRSFSNDETYVLLEKVIKLFTRVTEEFGAKLEVSYEMHIEAYETPLNHPVVMRFQEICEKLDIPCKLCSTFGGSDNNNIEHHGIKGLVLAAAMNSCHSNDEYTSVSELERITEVTIELMKGEIKKPRKLNSYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H9</accession>
    <name>F0T7H9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1305</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1305"/>
    <dbReference type="EnsemblBacteria" id="ADZ09547">
        <property type="protein sequence ID" value="ADZ09547"/>
        <property type="gene ID" value="Metbo_1305"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1305"/>
    <dbReference type="HOGENOM" id="CLU_3210746_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="44" mass="5250" checksum="E1675F163B220456" modified="2011-05-03" version="1">MVYKGISKADKLKPRVYQLLFTFMKRANEYKLTILIHKYRGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K4</accession>
    <name>F0T7K4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644923.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09572">
        <property type="protein sequence ID" value="ADZ09572"/>
        <property type="gene ID" value="Metbo_1331"/>
    </dbReference>
    <dbReference type="GeneID" id="10277782"/>
    <dbReference type="KEGG" id="mel:Metbo_1331"/>
    <dbReference type="eggNOG" id="arCOG03944">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978644_0_0_2"/>
    <dbReference type="OrthoDB" id="109844at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="284" mass="30890" checksum="A4B1096F3C3D0E91" modified="2011-05-03" version="1" precursor="true">MFGRTGHGGGIIKRKLFLSVLLLFGLALVLNVNFASATNVTTDKAKPKVTSVNPVNNSVVLSDKSKKVTIKVNFNEKIKSGTGSVELSANGKSKPVTTTVNGNTLTITSKNALSTSTKYSLIIHSNTVTDSSGNGNQLLRYTFTVSPISKAQMKDGISRVQSFYNKNNRLPNTVSFGAKKITISEFQKIIATQGLKIYTVKSKAVSNSNVKTVTAYGWNSCSKGWYKTGGTWKDYCPFCHSYNCLTYNPKHTYEGEWTCSKCDADFCNCGRCKASGSQVYLVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M3</accession>
    <name>F0T7M3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1351</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644942.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1351"/>
    <dbReference type="EnsemblBacteria" id="ADZ09591">
        <property type="protein sequence ID" value="ADZ09591"/>
        <property type="gene ID" value="Metbo_1351"/>
    </dbReference>
    <dbReference type="GeneID" id="24964552"/>
    <dbReference type="KEGG" id="mel:Metbo_1351"/>
    <dbReference type="eggNOG" id="arCOG02349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_0_2"/>
    <dbReference type="OrthoDB" id="35919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="149"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="247" mass="28511" checksum="6AA4A12E0FA0AACA" modified="2011-05-03" version="1">MSRKILIVEDEGILRVGTTLQLMSLGFEVVGHFQTGEEAVDNVVDLNPDLILMDIQLAGKMNGIETVREIQKKIDVPVVYLSAYSSHELIEEAETTKPFRYLVKPLDEMELKFTIETAIDTYQRQKRVEIKDEVLDNLHGLLYRFFVKDKKVNFINGSSESIIGLSERELTDFEGHFLEQFIIEEDQENVLNTMNNSLNSKVSFTMNYRIRNNNKINYFHEMGQPVYGDEGEITHIDGIIFDLNTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X5</accession>
    <name>F0T7X5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ordered locus">Metbo_0210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643813.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0210"/>
    <dbReference type="EnsemblBacteria" id="ADZ08462">
        <property type="protein sequence ID" value="ADZ08462"/>
        <property type="gene ID" value="Metbo_0210"/>
    </dbReference>
    <dbReference type="GeneID" id="10276636"/>
    <dbReference type="KEGG" id="mel:Metbo_0210"/>
    <dbReference type="eggNOG" id="arCOG00906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="OMA" id="CGEYTLQ"/>
    <dbReference type="OrthoDB" id="130364at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="SSF144210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="54" mass="6209" checksum="587E8765EA4D1AC6" modified="2011-05-03" version="1">MKPKMKKCKSCGEYTLTDKCPCGGEVGVIYPPKYSPEDKYGKYRRMLKEQLSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y2</accession>
    <name>F0T7Y2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08469">
        <property type="protein sequence ID" value="ADZ08469"/>
        <property type="gene ID" value="Metbo_0217"/>
    </dbReference>
    <dbReference type="GeneID" id="10276645"/>
    <dbReference type="KEGG" id="mel:Metbo_0217"/>
    <dbReference type="eggNOG" id="arCOG09730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143945_0_0_2"/>
    <dbReference type="OMA" id="QNSETLW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="15162" checksum="89E89B76B001C352" modified="2011-05-03" version="1" precursor="true">MLDKKILISIVIVLVIGVAAASYQATTKTPGVWQSSVAEQQLPTEQGSTGSDGSSALTSTSSSSGIVQKESQSSSSSGHSGSNGITTEQAKALSIAKGGIEDSSVTAGTPVLIGTSTYRVTLYNADGVDSGYFIVDTNTGKITEGAGGAPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T833</accession>
    <name>F0T833_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase_H_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645010.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1420"/>
    <dbReference type="EnsemblBacteria" id="ADZ09659">
        <property type="protein sequence ID" value="ADZ09659"/>
        <property type="gene ID" value="Metbo_1420"/>
    </dbReference>
    <dbReference type="GeneID" id="10277871"/>
    <dbReference type="KEGG" id="mel:Metbo_1420"/>
    <dbReference type="eggNOG" id="arCOG03130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_759948_0_0_2"/>
    <dbReference type="OMA" id="MVPEFYE"/>
    <dbReference type="OrthoDB" id="61485at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_H_2" evidence="1">
        <location>
            <begin position="146"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="317" mass="36461" checksum="56A1FAAD72E23995" modified="2011-05-03" version="1">MSFNGSLETEELRSKLIKEYMETSMDDAFNGEILETAHGSCYKISDSRKLKLNTLPKSKAKSNLLSDLKIIKGIGNFKEQKLNSEGYLTVEDLKEHPTYSGEACKLLELLDEENTSNITDYVTCRYPKSHPSALYSSCFSSDDNFLFMDIETLGLKSVPLILIGVSRFENKKITVDQFLLRDLKDEVSALEAFNSTVDEDTIFVSFNGQTFDIPYIKDRLNHHHIKTDLQRNHLDLMHFSRRAWKNHLPNCKLQTIEKHLFDFEREDDVPSSMVPSFYKTYLETGNIGTLIPIVEHNREDVITLARILSRLNTEMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T841</accession>
    <name>F0T841_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1429</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645018.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1429"/>
    <dbReference type="EnsemblBacteria" id="ADZ09667">
        <property type="protein sequence ID" value="ADZ09667"/>
        <property type="gene ID" value="Metbo_1429"/>
    </dbReference>
    <dbReference type="GeneID" id="24964556"/>
    <dbReference type="KEGG" id="mel:Metbo_1429"/>
    <dbReference type="eggNOG" id="arCOG02348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="TGMNDEK"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="131"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="207"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="341"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="394"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="468"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="634"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="728" mass="83089" checksum="B5F47DA84BE21F4E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T878</accession>
    <name>F0T878_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643841.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0238"/>
    <dbReference type="EnsemblBacteria" id="ADZ08490">
        <property type="protein sequence ID" value="ADZ08490"/>
        <property type="gene ID" value="Metbo_0238"/>
    </dbReference>
    <dbReference type="GeneID" id="10276668"/>
    <dbReference type="KEGG" id="mel:Metbo_0238"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_44_0_2"/>
    <dbReference type="OMA" id="FAMINSA"/>
    <dbReference type="OrthoDB" id="69240at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="16"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="29737" checksum="B8854E97F43E34D9" modified="2011-05-03" version="1">MKLVFDETGNENKESIVFLHPGGMSGWMWEQQIKHFKNYHCIIPDLPEHGRSASVKPFTITRTSEIVAKLVEDCPNERVNMVGIGLGGQIILQTMSKYPEIVDRAMVSGTLVRNHQYKSLIARLNELLMIYKSLKNNEFYIKAYMRTYNMPKVFFEKFKISTHQIGTSSIERILLENHTFKLPPILETDERKLLVMAGEKDYNIIKDSSKKIMNNYHARGALALGGGHLWNMETPDNFNEVLDAWLQNQPLPKALISPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C8</accession>
    <name>F0T8C8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_0288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643891.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0288"/>
    <dbReference type="EnsemblBacteria" id="ADZ08540">
        <property type="protein sequence ID" value="ADZ08540"/>
        <property type="gene ID" value="Metbo_0288"/>
    </dbReference>
    <dbReference type="GeneID" id="10276720"/>
    <dbReference type="KEGG" id="mel:Metbo_0288"/>
    <dbReference type="eggNOG" id="arCOG00813">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_0_2"/>
    <dbReference type="OMA" id="QNGWSYE"/>
    <dbReference type="OrthoDB" id="102435at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="12">
        <location>
            <begin position="350"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="426" mass="46565" checksum="417A31B4AF0EB44B" modified="2011-05-03" version="1">MKDTVNIGLIGFGTIGSGVVETLNKNIDLVENKVGKKVKLKKVVDLDITTDRGVEISSDVLSTDINDILDDPDIEIVIELMGGYQPALKFITKAMENGKHVVTANKALLAKHWDEIISTAQKNDVRICFEASVGGGIPLLQPLNEGLSANNIQSIYGIINGTANYILTKMTDEGLEFEDVLKEAQKKGYAEADPSFDIEGDDTAQKLIILTILSFGKYIKQDNFHVEGITCITPEDISFAKEELNSCIKLLAIAKLVDGKLEVRVHPTIVPLDHLLSSVNGVFNGVYLVGDVVGPVMMYGPGAGMMPTASAVVGDCIDIIENMEKPVLYGPKSCTVEKIKDISDLESKYYLRLITKDEPGVLHKISGILSKYNISIGSMTQKQHDKPGIPIFMVTHSALERDMRSAVEEIDQLKCVNDKTVFIRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E0</accession>
    <name>F0T8E0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0300"/>
    <dbReference type="EnsemblBacteria" id="ADZ08552">
        <property type="protein sequence ID" value="ADZ08552"/>
        <property type="gene ID" value="Metbo_0300"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0300"/>
    <dbReference type="eggNOG" id="arCOG00255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="10"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="195"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30483" checksum="7DFABB5F114A65C1" modified="2011-05-03" version="1">MIETTQKQELIGFFGPSGTFTEQAASQVGNDLLGFDSILDVLEAVKNGDVNLGVVPIENSIEGPVGVTLDLMVHDYDLKIKREIIIPISHNLLINTDASIEDIKYVYSHIQALSQCRKFTDSMGVVVNSTPSTSAAAEMVRGRKDSAAIGTRRAAEIYGLKIAASDIQDYKNNLTRFIVLGKTDHEFTGNDKTSVVFSLMEDKPGGLYHILELFAKLDINLTKIESRPSKEKLGNYIFFIDFDGHRTDESISNILDVIKSKVGFLKVLGSYPAQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G6</accession>
    <name>F0T8G6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1483</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09717">
        <property type="protein sequence ID" value="ADZ09717"/>
        <property type="gene ID" value="Metbo_1483"/>
    </dbReference>
    <dbReference type="GeneID" id="10277934"/>
    <dbReference type="KEGG" id="mel:Metbo_1483"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444562_0_0_2"/>
    <dbReference type="OMA" id="VTCSTFT"/>
    <dbReference type="OrthoDB" id="58020at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
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        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
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        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="POMP_repeat"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="614" mass="63834" checksum="EE6594339A0677CF" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H1</accession>
    <name>F0T8H1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Metbo_1489"/>
    </dbReference>
    <dbReference type="GeneID" id="10277940"/>
    <dbReference type="KEGG" id="mel:Metbo_1489"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157711_1_0_2"/>
    <dbReference type="OMA" id="SVCANER"/>
    <dbReference type="OrthoDB" id="112624at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020075">
        <property type="entry name" value="Uncharacterised_AF2234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10967">
        <property type="entry name" value="DUF2769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="12217" checksum="AE99FA310FD9E070" modified="2011-05-03" version="1">MTIVDYDKKNLERCLCVKCPVQMESVCANERKLGVLDLLKNLKENDLMPEPTHIAGLYCSIGKSTCADIDTDQECQCAKCPVFKEYSLKTGYYCKLGKEEDILKNDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I0</accession>
    <name>F0T8I0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SOUL heme-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645082.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09731">
        <property type="protein sequence ID" value="ADZ09731"/>
        <property type="gene ID" value="Metbo_1499"/>
    </dbReference>
    <dbReference type="GeneID" id="10277950"/>
    <dbReference type="KEGG" id="mel:Metbo_1499"/>
    <dbReference type="eggNOG" id="arCOG04820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068699_0_1_2"/>
    <dbReference type="OrthoDB" id="96329at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_haem-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="PTHR11220"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="23142" checksum="6AAA0A345F717A56" modified="2011-05-03" version="1">MVETLAYEVESKDGNFEIRKYGDHILAHVDIEAPFNEAMNMGFSVLANYIFGGNKKRSSIEMTAPVEEEKRKSEKIPMTAPVTEESLKESEKIKMTTPVTEEENGNIHRISFVMPSKYTMEALPEPEDERIKFEEIKEEKMAVLKFKGRVKEKLAAEKIEEMKRWLKENNIQPESNFVVAQYNHPAVPGFLRRNEIMVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K0</accession>
    <name>F0T8K0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1521</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645102.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1521"/>
    <dbReference type="EnsemblBacteria" id="ADZ09751">
        <property type="protein sequence ID" value="ADZ09751"/>
        <property type="gene ID" value="Metbo_1521"/>
    </dbReference>
    <dbReference type="GeneID" id="10277972"/>
    <dbReference type="KEGG" id="mel:Metbo_1521"/>
    <dbReference type="eggNOG" id="arCOG09456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110620_1_0_2"/>
    <dbReference type="OMA" id="HHLLALW"/>
    <dbReference type="OrthoDB" id="139622at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17281" checksum="57B82E52CCA9DCB3" modified="2011-05-03" version="1">MRDKRFVSQHRGGPLTREQHFQLIQWACEVAQHVLYLYGETTNPRLLNALKVANDWKEGKASVGDARYASVDAIKVANEASNTVQTMVARSVGHAVATAHMADHSIVAAWYALKAVKIADKSVDNERIWQNNQLPPKIKELVLSARMDSKFEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P1</accession>
    <name>F0T8P1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0331</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0331"/>
    <dbReference type="EnsemblBacteria" id="ADZ08583">
        <property type="protein sequence ID" value="ADZ08583"/>
        <property type="gene ID" value="Metbo_0331"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0331"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OMA" id="HVSGDYD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="19"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="19118" checksum="1078AC1A9B0ABAEB" modified="2011-05-03" version="1">MFNNGEIMKNNDENQIVKIDETDKKILNLFNEDGRMSYRKISRKLDISIGTVHNRIEKLVKSGVIKKFAPVIDHSKLGYNFTTIIGVRVKGGVLKNWEDKTSYHKNVLCMYDVTGEFDALIVARFKDTTELDEFVKNLLQEPDVQRTYTQTVLNILKEDLGSSKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q7</accession>
    <name>F0T8Q7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0347"/>
    <dbReference type="EnsemblBacteria" id="ADZ08599">
        <property type="protein sequence ID" value="ADZ08599"/>
        <property type="gene ID" value="Metbo_0347"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0347"/>
    <dbReference type="eggNOG" id="arCOG01156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_0_1_2"/>
    <dbReference type="OMA" id="DTHYGPI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="145"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="386" mass="43713" checksum="36DB0EE5CBE64534" modified="2011-05-03" version="1">MRTIIRFAGFMSFFFLGFLAVNYTIFYGTYTLFGVEPGLGFYTLLVIAAVSYPLAAMIERVVSNNYTRIFYTAASSWMGISFYVLTFMAVYWILSIFIKIPGEAAGTIIIVLSALLSGYSLINSSRIDIKHVKIPLKGLERQVKAVQLSDIHIGSIRNSEYLEEVVEKTNKLDPEVVFITGDMVDGSARLHTHTFNAINKLKAPVFFIMGNHETYEGLDEVLRVLNGVKMKILRDQKVEFNGIQIIGVEYSFERNHMKNVLSKVDLDPSKPSIVLYHTPTELEATADADVGLQLSGHTHAGQMLPFNYLVRLMFRYMNGIYKYKDTYLYVSPGTGTWGPPMRLGSRCEITALDLEASKPKTNPKESLMKYKKINQWHSKKLLETQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U4</accession>
    <name>F0T8U4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hypoth_Ymh domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645123.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09772">
        <property type="protein sequence ID" value="ADZ09772"/>
        <property type="gene ID" value="Metbo_1542"/>
    </dbReference>
    <dbReference type="GeneID" id="10277997"/>
    <dbReference type="KEGG" id="mel:Metbo_1542"/>
    <dbReference type="eggNOG" id="arCOG11925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948677_0_0_2"/>
    <dbReference type="OrthoDB" id="50706at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012654">
        <property type="entry name" value="CHP02391"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09509">
        <property type="entry name" value="Hypoth_Ymh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02391">
        <property type="entry name" value="hypoth_ymh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hypoth_Ymh" evidence="1">
        <location>
            <begin position="162"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="33913" checksum="63D5355D68312D9D" modified="2011-05-03" version="1">MFYQLLIDFLENFFGGGFILFDKESNRIYLRLYNKINEIIGGNCFEENLVAVDFPFIHLRSLDPDAPETDWIYGGQQLCGEQLSSIEDLFIKTGENVYELDEEDSNLISSINDYMEDISNYKKEENLRLLKIAKERTKHANTLSNSKKIMEENNIIDFWDLIHENIVKVSKSRFNNEHYADAVESAFKEVNIRVKRIVENKIGQELDGKHLMDTAFSFSNTSNPTIILDDLSKKTGKNIQRGYKQLFSGSMQAIRNPKAHENIEINKNEAIHFIFLASSLMFKLDNAIEINED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y9</accession>
    <name>F0T8Y9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645168.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09817">
        <property type="protein sequence ID" value="ADZ09817"/>
        <property type="gene ID" value="Metbo_1590"/>
    </dbReference>
    <dbReference type="GeneID" id="10278047"/>
    <dbReference type="KEGG" id="mel:Metbo_1590"/>
    <dbReference type="HOGENOM" id="CLU_2581442_0_0_2"/>
    <dbReference type="OrthoDB" id="141442at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="9268" checksum="8DC8E944A2A29F65" modified="2011-05-03" version="1">MDENTKKKILKLLSKAKKENPENPAVSCNDLEDELNMDDHELDVYIRDLEYRGYIEYEGSKTYDGQDITLTVKGEKIAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T939</accession>
    <name>F0T939_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644012.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0409"/>
    <dbReference type="EnsemblBacteria" id="ADZ08661">
        <property type="protein sequence ID" value="ADZ08661"/>
        <property type="gene ID" value="Metbo_0409"/>
    </dbReference>
    <dbReference type="GeneID" id="10276846"/>
    <dbReference type="KEGG" id="mel:Metbo_0409"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="DYAIFAM"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="40"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="115"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="374" mass="43177" checksum="B8BAC655C970D867" modified="2011-05-03" version="1">MPFNPDEHPDWNTLRDKIIGLGESSIQKSYYPELQKRLAELERFRSLLDETNDHIFLLNIPSGKFSDVNKSASERFGLNSKEVPITSIHDLVDKDEIEDFNELFNQLANKSQDRKTFYSMFLDSGGIKIPVEISASTVNFGDELYIVMVVRDITERKEAENKIKKSLEEKKVLLREIHHRVKNNMQIISSLLNLQTTYTNDENVVDILMESQNRVKSMAMIHEKLYQSTELARIDFRDYIYQLTMFLRQSYVYMNSSITIETEVDDIHLNIDTAVPCGLIINELVTNSIKHGFPNGMPGLIRVKLFESNGEYTLCVKDNGVGFPETIDFRNTDTLGLQLITNLVLQLDGTIDVIFDHGTEFKINFQPMEYIQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T957</accession>
    <name>F0T957_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MmgE/PrpD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0427</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644030.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0427"/>
    <dbReference type="EnsemblBacteria" id="ADZ08679">
        <property type="protein sequence ID" value="ADZ08679"/>
        <property type="gene ID" value="Metbo_0427"/>
    </dbReference>
    <dbReference type="GeneID" id="10276864"/>
    <dbReference type="KEGG" id="mel:Metbo_0427"/>
    <dbReference type="eggNOG" id="arCOG04285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_1_2"/>
    <dbReference type="OMA" id="ECTKHLG"/>
    <dbReference type="OrthoDB" id="26171at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="PTHR16943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="SSF103378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE_PrpD" evidence="2">
        <location>
            <begin position="4"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE_PrpD_C" evidence="3">
        <location>
            <begin position="266"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="444" mass="48702" checksum="2500221C36594301" modified="2011-05-03" version="1">MITEDLASKIAEINYDELPKEVVSQAKLCFLDFLAVAMRGSNSPSGKIVKEIFNCAKDEVIVDLDKLSVMDAAFCNGVFAHSLDLDDGHRHAQLHPGCAVIPASLAAADFHKKTGKELLEAIVAGYQVSILMGKLSNPMHRNNGFHSTGTCGTLGAAAAVCKIMELNEEKIINALGLAGTQAAGLLESDHAGTMGKHLHAGKAAHAGVIAAVLAEKGFTGAKSIIDGKQGFYMAMVSDSDISILENSWKEMDKDRYHILDIYFKQYPLCRHLHSSIDAARFIKDKMETEGSNLDDIRRIYIKTYKIAAEHDNYQPETVEDLRQSLPFAVAISLIKGAPNTENMEITDLTRSLASKTVIETDAKLDGSYPSKRPSEISVETGNGVYNCQVDLPLGEPENPLDLQDLTQKFQELNPHVNVELMNPIEQIESIYIHDFMEMLNLNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T962</accession>
    <name>F0T962_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">V-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0432</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644035.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0432"/>
    <dbReference type="EnsemblBacteria" id="ADZ08684">
        <property type="protein sequence ID" value="ADZ08684"/>
        <property type="gene ID" value="Metbo_0432"/>
    </dbReference>
    <dbReference type="GeneID" id="10276869"/>
    <dbReference type="KEGG" id="mel:Metbo_0432"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_1_0_2"/>
    <dbReference type="OMA" id="MAVNIMA"/>
    <dbReference type="OrthoDB" id="14889at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="PTHR11629"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="121"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="661" mass="73362" checksum="E198130B9280D8E5" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F0</accession>
    <name>F0T9F0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645252.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1675"/>
    <dbReference type="EnsemblBacteria" id="ADZ09901">
        <property type="protein sequence ID" value="ADZ09901"/>
        <property type="gene ID" value="Metbo_1675"/>
    </dbReference>
    <dbReference type="GeneID" id="10278132"/>
    <dbReference type="KEGG" id="mel:Metbo_1675"/>
    <dbReference type="eggNOG" id="arCOG01151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010534_0_0_2"/>
    <dbReference type="OMA" id="WELANNR"/>
    <dbReference type="OrthoDB" id="88711at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="PTHR34990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="29949" checksum="73A2CAE3A07BA2B9" modified="2011-05-03" version="1">MMLVISDLHLGYEKSNKDAFYNFLDDYNTKLDHLIILGDFFDFWRKNNSEIVLENEKILEKLLDLEAKKIHYIIGNHDYYMLKLKERYGDNFPFEVKRNLRLEDSGKKFYFTHGYEFEAIQLEPFTIDMYEDFSEKMCFSEDFIGGVAGQIWDIIQGSDIKERLEKDPRKRFETSEESLQIYKMAISTGKCYVFGIEPHERLVFGHTHGPFINQSKTVVNSGSWTDELKNKDYQNSYVEIDDGNMDLKFYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H1</accession>
    <name>F0T9H1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA decarbonylase/synthase complex subunit gamma</fullName>
            <shortName evidence="5">ACDS complex subunit gamma</shortName>
            <ecNumber evidence="5">2.1.1.245</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">ACDS complex methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Corrinoid/iron-sulfur component large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cdhE</name>
        <name evidence="9" type="ordered locus">Metbo_0467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,6,7,8-tetrahydrosarcinapterin + methyl-Co(III)-[corrinoid Fe-S protein] = 5-methyltetrahydrosarcinapterin + Co(I)-[corrinoid Fe-S protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45196"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59924"/>
            <dbReference type="ChEBI" id="CHEBI:64267"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.1.1.245"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>corrinoid</name>
            <dbReference type="ChEBI" id="CHEBI:33913"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <dbReference type="EC" id="2.1.1.245" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644070.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0467"/>
    <dbReference type="EnsemblBacteria" id="ADZ08719">
        <property type="protein sequence ID" value="ADZ08719"/>
        <property type="gene ID" value="Metbo_0467"/>
    </dbReference>
    <dbReference type="GeneID" id="10276905"/>
    <dbReference type="KEGG" id="mel:Metbo_0467"/>
    <dbReference type="eggNOG" id="arCOG01979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050002_0_0_2"/>
    <dbReference type="OMA" id="SCMAFAT"/>
    <dbReference type="OrthoDB" id="31154at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046356">
        <property type="term" value="P:acetyl-CoA catabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01136">
        <property type="entry name" value="CdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016218">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023427">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000376">
        <property type="entry name" value="AcCoA_decarb_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4Fe-4S" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methoxybenzimidazolylcob(I)amide binding" evidence="6">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>5-methoxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:157765"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000376-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000376-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="462" mass="51094" checksum="5839F40C6E405AFE" modified="2011-05-03" version="1">MQVTAMEIYRLLPKTNCAKCGEASCMAFATKLSDKETDLELCTQLAANEMEELENLLAPAVREITIGKRDKTFVVGGDEVLYRYELTYYNQTPIIIDVNDDMDEEALAQRVKIIEESEFERTGETLVLDGIALRNSSGNADKFAETASKLKSSKLPLVLCSLDPVAMKAALENVGDERPLIYAATENNFDEMAELALEYDCPLAIFVPNDLEKMKEISNILRSKGVHELVLDPGTYVNDGIGDTLDNFVMIRRLAIEERDEDFRFPIMGIPALTWLYEKDEIQGGIKEATVAATLMNKYADMLIIHGTNIWELIPVLTLRQSVFTDPRKPQAVDAGLYEFGELNEDSPVALTTNFALTYYTVEGDLKSGKSNCYLLVLDTEGRAVDVSLAGGQLTGASVAELIKDSGIEDKVKTRTLIIPGLAAPISGEIEDDSGWEVLVGPRDSSGMPDFFHKLEEKQEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J3</accession>
    <name>F0T9J3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate ferredoxin/flavodoxin oxidoreductase, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0489</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644092.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0489"/>
    <dbReference type="EnsemblBacteria" id="ADZ08741">
        <property type="protein sequence ID" value="ADZ08741"/>
        <property type="gene ID" value="Metbo_0489"/>
    </dbReference>
    <dbReference type="GeneID" id="10276929"/>
    <dbReference type="KEGG" id="mel:Metbo_0489"/>
    <dbReference type="eggNOG" id="arCOG01604">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="OMA" id="GSWRTFK"/>
    <dbReference type="OrthoDB" id="125632at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PTHR43724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="8964" checksum="A0EDDBD5B9350B25" modified="2011-05-03" version="1">MVAIGAAVKEPGSTRKNKTGSWRTFKPILDKETCIDCENCVMFCPEGCIDKNYDIDYDYCKGCGICAEECPVKAIKMERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L7</accession>
    <name>F0T9L7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644116.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0513"/>
    <dbReference type="EnsemblBacteria" id="ADZ08765">
        <property type="protein sequence ID" value="ADZ08765"/>
        <property type="gene ID" value="Metbo_0513"/>
    </dbReference>
    <dbReference type="GeneID" id="10276958"/>
    <dbReference type="KEGG" id="mel:Metbo_0513"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_6_2_2"/>
    <dbReference type="OMA" id="SHEAVMH"/>
    <dbReference type="OrthoDB" id="111072at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="268" mass="28046" checksum="4819D7BCB29F5009" modified="2011-05-03" version="1">MDILLYIIALIITGAMVGFASGLLGVGGGFIMVPVQFFLLTSIGVDPTIAIRVAFGTSLAVILPTAISGTIGHKRRNAVLVRPTILMGISGVLASFAGGTLATNIPGDYLKLAFGVLVFLAAVWMLVAKYPDNDTKPKEGVIPYILIGLFAGSLSGLLGIGGGIVLVPILVFLMNFNLRTAIGTSTAVLVFTAIGGVVSYIINGINVPGIPPYSIGYINLVQLVLLACISTPVAQLGVRVSHNIPEKQLKYIYIVVMIYISLKMMGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P4</accession>
    <name>F0T9P4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645274.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1698"/>
    <dbReference type="EnsemblBacteria" id="ADZ09923">
        <property type="protein sequence ID" value="ADZ09923"/>
        <property type="gene ID" value="Metbo_1698"/>
    </dbReference>
    <dbReference type="GeneID" id="10278155"/>
    <dbReference type="KEGG" id="mel:Metbo_1698"/>
    <dbReference type="eggNOG" id="arCOG02967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098571_0_0_2"/>
    <dbReference type="OMA" id="IKIYEAI"/>
    <dbReference type="OrthoDB" id="87674at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="278" mass="31414" checksum="794E5200D389DEAC" modified="2011-05-03" version="1">MDQSKIKQINREKRGQITDEYLSQFKELSDESIIQMLKNKNPQKRTAAAKILGKTLNSKNVAILCGAMKNEKAIYSRIAMSESLGEMGEIAVPNLIELLGKIGNNQEKELPTKYFKKKNFPLQRDLAARTLVKVGKPALPHLIQTITSTHEVFTKEQAVDAVGGITYKTMDQSAVDTIIQTIHENTKNSDNTLLLWKCVRALSGFQKNRRAFDLLMEMLEHETKPPILWETLRSLGKVGISNTQLTTIVENFETENPEIKLAYDGMLTDLNLKIKIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q0</accession>
    <name>F0T9Q0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1704</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645280.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1704"/>
    <dbReference type="EnsemblBacteria" id="ADZ09929">
        <property type="protein sequence ID" value="ADZ09929"/>
        <property type="gene ID" value="Metbo_1704"/>
    </dbReference>
    <dbReference type="GeneID" id="10278161"/>
    <dbReference type="KEGG" id="mel:Metbo_1704"/>
    <dbReference type="eggNOG" id="arCOG01006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_0_2"/>
    <dbReference type="OMA" id="RIDLMAY"/>
    <dbReference type="OrthoDB" id="73309at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="27924" checksum="6022ADC5AB3582F3" modified="2011-05-03" version="1">MLEIFQYPFMQKAIVAAVLISVACGVVGSYVVIKRIVSLSGAISHAAFGGVGLGYFIGVNPVLAAIPFSIVSAMAIGGVRQVAKISEDTAIGILWSVGMAIGVIFINLTPGYAPDLFSYLFGSILTVSNSDLLIMLVLDMIIVAVVYIFRREFLAVSFDEEFSNVTGVHALAIYLLLLALVALSVVVLIKAVGVILLIALFTIPAAIAKQYSYNIRNLMILATVLGIILTTTGLVLSYIFDLASGATIVMVLAAAFAISYYLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R2</accession>
    <name>F0T9R2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipolytic protein G-D-S-L family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645292.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1717"/>
    <dbReference type="EnsemblBacteria" id="ADZ09941">
        <property type="protein sequence ID" value="ADZ09941"/>
        <property type="gene ID" value="Metbo_1717"/>
    </dbReference>
    <dbReference type="GeneID" id="10278174"/>
    <dbReference type="KEGG" id="mel:Metbo_1717"/>
    <dbReference type="eggNOG" id="arCOG05121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088167_0_0_2"/>
    <dbReference type="OrthoDB" id="92863at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="9"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="23720" checksum="DFF43B1CE17FC3D2" modified="2011-05-03" version="1">MIKRRRVLCFGDSITWGFNPMDQMRMNEDKRWTGILDSGLGDEYTVIEEGLNSRTTVWDDPLKGSQKNGLKYLIPCLASHKPLDLVVLMLGTNDMKHRFSLPTTEIARGINVLVEVILRSESGTNGLSPEILLLAPPFLLEATGFDDEFRNSYNKSQKLPGYVEKIAKEHGCYFLDTSKIMVASELDGVHPAVVEHKKLGRALIKEVSSILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W9</accession>
    <name>F0T9W9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps19e</name>
        <name evidence="5" type="ordered locus">Metbo_0540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644143.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0540"/>
    <dbReference type="EnsemblBacteria" id="ADZ08792">
        <property type="protein sequence ID" value="ADZ08792"/>
        <property type="gene ID" value="Metbo_0540"/>
    </dbReference>
    <dbReference type="GeneID" id="10276985"/>
    <dbReference type="KEGG" id="mel:Metbo_0540"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OMA" id="YIDGPVG"/>
    <dbReference type="OrthoDB" id="92409at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_S19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_S19e_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_S19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="PTHR11710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="16302" checksum="49B891C9DE1A9C0F" modified="2011-05-03" version="1">MTTIYDVPADSLISEVAKELNENKSINTPEWATFVKTGVHKERRPDNPDWWYVRCASVLRKVYMDGPVGVNSLKTYYGGKKDRGTSPEKFKKGSGAVIRGALHQLEDAGYIRKVGEGRLVTPEGKSFLDKTSHKIIKDIPELSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y2</accession>
    <name>F0T9Y2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08805">
        <property type="protein sequence ID" value="ADZ08805"/>
        <property type="gene ID" value="Metbo_0553"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0553"/>
    <dbReference type="eggNOG" id="arCOG10441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_216164_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5687" checksum="6FF713852DD7C407" modified="2011-05-03" version="1">MPVNQNETNLQAITVTIAHLEKQEDRDEEMLKKLKHERQSILKQMNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y4</accession>
    <name>F0T9Y4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable S-methyl-5'-thioinosine phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioinosine phosphorylase</fullName>
            <shortName evidence="1">MTI phosphorylase</shortName>
            <shortName evidence="1">MTIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + S-methyl-5'-thioinosine = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:30643"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.44" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644158.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0555"/>
    <dbReference type="EnsemblBacteria" id="ADZ08807">
        <property type="protein sequence ID" value="ADZ08807"/>
        <property type="gene ID" value="Metbo_0555"/>
    </dbReference>
    <dbReference type="GeneID" id="10277000"/>
    <dbReference type="KEGG" id="mel:Metbo_0555"/>
    <dbReference type="eggNOG" id="arCOG01327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="OMA" id="MTQCPEA"/>
    <dbReference type="OrthoDB" id="63298at2157"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="1">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="27952" checksum="343A7E0A11B26F63" modified="2011-05-03" version="1">MIGIIGGTGIYEIVTLGDEIDTKVIKTPYGKSPEVSIFELHGKDVVFMPRHAKGHSNPPHMVNYRANVYAMKELGVERIIATNAVGSLEKTVKPGDFMVPNDFIDFTKLRPGTFYDSRTVHVDVTEPYCNYMRRIISSSGDVVENGVYVCTEGPRFETPAEIQMFKLMGGTVVGMTGIPEAVLARELEICYASICTVSNYAASISPNNLTIDEVFEIMEKKKHDLVSLMDTAISKMGEERDCDCKHALKGAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y9</accession>
    <name>F0T9Y9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0560"/>
    <dbReference type="EnsemblBacteria" id="ADZ08812">
        <property type="protein sequence ID" value="ADZ08812"/>
        <property type="gene ID" value="Metbo_0560"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0560"/>
    <dbReference type="eggNOG" id="arCOG04055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="OMA" id="WLSELLY"/>
    <dbReference type="OrthoDB" id="125788at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="PTHR12682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="SSF69819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="6">
        <location>
            <begin position="13"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17899" checksum="41B640D506ECC45C" modified="2011-05-03" version="1">MFTKNDSEENLKYEFFDVTADVGFKAYGDTIDVAFSNAAIAMFEVITDTSKVEHRIIKKIKIEAEDECSTLYEWLSELLFLHDTENLVFSKFNVKLYRKLKNETKIFYLEALAFGEQFNPEVHEKRSEVKAVTYHMMEVNLEDTCELQVILDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA31</accession>
    <name>F0TA31_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1758</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645332.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1758"/>
    <dbReference type="EnsemblBacteria" id="ADZ09981">
        <property type="protein sequence ID" value="ADZ09981"/>
        <property type="gene ID" value="Metbo_1758"/>
    </dbReference>
    <dbReference type="GeneID" id="10278215"/>
    <dbReference type="KEGG" id="mel:Metbo_1758"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_113_1_2"/>
    <dbReference type="OMA" id="INIPMIF"/>
    <dbReference type="OrthoDB" id="18304at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038318">
        <property type="entry name" value="KdpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025201">
        <property type="entry name" value="KdpD_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003852">
        <property type="entry name" value="Sig_transdc_His_kinase_KdpD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13493">
        <property type="entry name" value="DUF4118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13492">
        <property type="entry name" value="GAF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02702">
        <property type="entry name" value="KdpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="KdpD" evidence="8">
        <location>
            <begin position="27"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Usp" evidence="7">
        <location>
            <begin position="257"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4118" evidence="10">
        <location>
            <begin position="406"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="9">
        <location>
            <begin position="531"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="651" mass="74099" checksum="5FE872CF7495867F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA35</accession>
    <name>F0TA35_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645336.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1762"/>
    <dbReference type="EnsemblBacteria" id="ADZ09985">
        <property type="protein sequence ID" value="ADZ09985"/>
        <property type="gene ID" value="Metbo_1762"/>
    </dbReference>
    <dbReference type="GeneID" id="10278219"/>
    <dbReference type="KEGG" id="mel:Metbo_1762"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_8_5_2"/>
    <dbReference type="OMA" id="IERNDTH"/>
    <dbReference type="OrthoDB" id="108184at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="13055" checksum="8EFAD0E91AC929B5" modified="2011-05-03" version="1">MKVKYATIAVSDMEESVKFYTEVMGFNVDHTLNPYPGFNITFLANEGDAMVELIENKNEPQTPGIFLIGMEVEDMDKTAAELIDKGAEFTRGPLEVGDGAKLAFLKDPNGVEIELIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA62</accession>
    <name>F0TA62_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645363.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10012">
        <property type="protein sequence ID" value="ADZ10012"/>
        <property type="gene ID" value="Metbo_1790"/>
    </dbReference>
    <dbReference type="GeneID" id="10278247"/>
    <dbReference type="KEGG" id="mel:Metbo_1790"/>
    <dbReference type="eggNOG" id="arCOG06478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133049_1_0_2"/>
    <dbReference type="OMA" id="ICMIMEG"/>
    <dbReference type="OrthoDB" id="129151at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="18141" checksum="E14D00785C998F29" modified="2011-05-03" version="1">MAKEGNVLLGILAIILGILVIIFPLISVFTVSLIAGIGILFLGIWLLIQSFGVWASNKGVSISYLILGILAIIVGIGLFGNVLAFSFLVSLALYIAGFFLLISGFLSLFSKEGTAGKGSGVLGIILGILYMILAAYAWNPIYLAFLIGIWLIIDGIALFFVNPMEVAEAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA78</accession>
    <name>F0TA78_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Metbo_1806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1806"/>
    <dbReference type="EnsemblBacteria" id="ADZ10028">
        <property type="protein sequence ID" value="ADZ10028"/>
        <property type="gene ID" value="Metbo_1806"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1806"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054151_0_0_2"/>
    <dbReference type="OMA" id="WAQVWIR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="2">
        <location>
            <begin position="331"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="419" mass="45187" checksum="915B8B2627EA5309" modified="2011-05-03" version="1">MTGGGIINRKSISILLLLLTIAIPLNVDAISAAATSQTNTDQKTSPSVTNNYIQNTTTKTSNTTTTNKSQVSTTKNTTTKNYAAAGTVKTVSSTKTTSLSFKNIVAASTSVKNFINTNKRLPSYVTISGVKITMPQMLYYLSQAIVKINSGTKTSSTLITVKAPTVNNTETVKAGNLTKNQYLSEASSVYNYILKNRMAPSTISSSLGKIRYESAIFSFSKVVSFYGTNTRLPKFVAIVPLAKSTVKPSTGTSTNGSSSGYVENVVPTSLKSYLLPTANCQSDNSQIKTLAASITKGLNTPMQRATAIYNWVRDHISYSFYYNTQKGALGTLSARTANCVDTAHLVVALERAAGFAAQYEHVTAQFSSGTWYGHVIALVYVNGVWYKADGTSYRNSFGVVNNWNTNTATVHGKYRELPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA93</accession>
    <name>F0TA93_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1821"/>
    <dbReference type="EnsemblBacteria" id="ADZ10043">
        <property type="protein sequence ID" value="ADZ10043"/>
        <property type="gene ID" value="Metbo_1821"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1821"/>
    <dbReference type="HOGENOM" id="CLU_3401572_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="30" mass="3449" checksum="50E069C43B825621" modified="2011-05-03" version="1">MADKKVQTDTLNKRSLKKTGTKDLKEKTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA7</accession>
    <name>F0TAA7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1 3">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name evidence="5" type="ordered locus">Metbo_0608</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1 3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644210.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0608"/>
    <dbReference type="EnsemblBacteria" id="ADZ08859">
        <property type="protein sequence ID" value="ADZ08859"/>
        <property type="gene ID" value="Metbo_0608"/>
    </dbReference>
    <dbReference type="GeneID" id="10277053"/>
    <dbReference type="KEGG" id="mel:Metbo_0608"/>
    <dbReference type="eggNOG" id="arCOG04313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="106462at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NDK" evidence="4">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17126" checksum="1CF439B97F92A103" modified="2011-05-03" version="1">MKQRSFVMLKPDAVKRRLAGEVLGRFENRGLKILAAKLLVITPELAKEHYGEHSERPFFKDLVEYITSGPVLATVIEGEECISLIRKMVGATNPQEADLGTIRGDYAIDTGRNIIHASDSETSAAREIALFFEDSEIYDYSMPDEEMIYEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD0</accession>
    <name>F0TAD0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08882">
        <property type="protein sequence ID" value="ADZ08882"/>
        <property type="gene ID" value="Metbo_0631"/>
    </dbReference>
    <dbReference type="GeneID" id="10277076"/>
    <dbReference type="KEGG" id="mel:Metbo_0631"/>
    <dbReference type="eggNOG" id="arCOG04920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182218_0_0_2"/>
    <dbReference type="OMA" id="PHGIKVM"/>
    <dbReference type="OrthoDB" id="121990at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019240">
        <property type="entry name" value="DUF2196"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40069">
        <property type="entry name" value="PTHR40069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09962">
        <property type="entry name" value="DUF2196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03833">
        <property type="entry name" value="TIGR03833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="66" mass="7354" checksum="735B1F4A499B97B8" modified="2011-05-03" version="1">MNGKNRKDIKPGKEVFIVLKKDQRTGKRSKGVVADILTNKSFHPHGIKVRLTDGRVGRVQEIVGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE1</accession>
    <name>F0TAE1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644244.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0642"/>
    <dbReference type="EnsemblBacteria" id="ADZ08893">
        <property type="protein sequence ID" value="ADZ08893"/>
        <property type="gene ID" value="Metbo_0642"/>
    </dbReference>
    <dbReference type="GeneID" id="10277087"/>
    <dbReference type="KEGG" id="mel:Metbo_0642"/>
    <dbReference type="eggNOG" id="arCOG02461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="CLSCHEI"/>
    <dbReference type="OrthoDB" id="110830at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="83" mass="9330" checksum="1EAF7857764A544E" modified="2011-05-03" version="1">MVEIEIDENACVGCGSCVDDCPNDVYEMDEAKNKTKVVNADDCMACLSCHEICPAEAMEHKNIHVAKRLYIDRRVNDILNKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE3</accession>
    <name>F0TAE3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0644</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0644"/>
    <dbReference type="EnsemblBacteria" id="ADZ08895">
        <property type="protein sequence ID" value="ADZ08895"/>
        <property type="gene ID" value="Metbo_0644"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0644"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_1_0_2"/>
    <dbReference type="OMA" id="NNDHYWQ"/>
    <dbReference type="OrthoDB" id="58511at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="PTHR30221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="27937" checksum="B1C2841734DB6B99" modified="2011-05-03" version="1" precursor="true">MTIESILSNPLLKDVVVSLIIIGAALGIVKWTSIFITKTGTKFDSEATLIQVVNEIIRYSVYAIALTLILNELGINITAIALSLGIVGIAVGFAARDTISNFIAGLFVLADKSFKVGDIIEISDYKGKVHIMGFRVTKLITADNNIVSIPNSNFSKNVHVNHTPLEEKRVDLAITVPYQVKVEGLREKFIEILDKHEWALDDPLPNFNINELADTGIKSTLSFWVVDPWKVEEYRSIIASEIGTLLVVDIEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH7</accession>
    <name>F0TAH7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644280.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0678"/>
    <dbReference type="EnsemblBacteria" id="ADZ08929">
        <property type="protein sequence ID" value="ADZ08929"/>
        <property type="gene ID" value="Metbo_0678"/>
    </dbReference>
    <dbReference type="GeneID" id="10277123"/>
    <dbReference type="KEGG" id="mel:Metbo_0678"/>
    <dbReference type="eggNOG" id="arCOG06415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202923_1_0_2"/>
    <dbReference type="OrthoDB" id="123772at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="SSF57903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="5792" checksum="4F74653ACDB65421" modified="2011-05-03" version="1">MELTGICSLCGKTGKIHTCQLCGSLVCEECYNPSKGICRSCNKRNIPDIKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI8</accession>
    <name>F0TAI8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645411.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1838"/>
    <dbReference type="EnsemblBacteria" id="ADZ10060">
        <property type="protein sequence ID" value="ADZ10060"/>
        <property type="gene ID" value="Metbo_1838"/>
    </dbReference>
    <dbReference type="GeneID" id="10278295"/>
    <dbReference type="KEGG" id="mel:Metbo_1838"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_1_2"/>
    <dbReference type="OMA" id="PERHEKT"/>
    <dbReference type="OrthoDB" id="121018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029478">
        <property type="entry name" value="TM1586_NiRdase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14512">
        <property type="entry name" value="TM1586_NiRdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="TM1586_NiRdase" evidence="2">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="19154" checksum="DE3C79491BE8B041" modified="2011-05-03" version="1">MDVFEAIESRRSIRKYLTKEVEQDKIDKILESARIAPSAANRQEWKFVVVKDQQSRDKLVDACMGQKFVAQAPVFIAACSTESEKVMPCGQYAYSVDLSIALSFMILEATELGLGTCWLGAFKEDEVKSILNIPEEVRLVGLTTLGYSDESPDKRPRKSFEEVVTYEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI9</accession>
    <name>F0TAI9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645412.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1839"/>
    <dbReference type="EnsemblBacteria" id="ADZ10061">
        <property type="protein sequence ID" value="ADZ10061"/>
        <property type="gene ID" value="Metbo_1839"/>
    </dbReference>
    <dbReference type="GeneID" id="10278296"/>
    <dbReference type="KEGG" id="mel:Metbo_1839"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_9_1_2"/>
    <dbReference type="OrthoDB" id="121018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="220" mass="25335" checksum="7FDC7D4FAC52D640" modified="2011-05-03" version="1">MNVSDALKSRFTCRSFKQKPVSREKITEILEEALHAPSWANTQPWEIYVANAEKLDKIRAEYLEYFDQDKPRNPDVALARTWPDKYQKRIRDLADEMYNHLKISGTDEKERNASWRSNFEFFNAPAVIYLCMNQELGEWSMFDMGAISQSIMLAATEAGLDSAPAINLVVHPEILRSELDIPMELSIVLGIALGYKNDEAPQSSFKSSRRSLEEVVKYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ7</accession>
    <name>F0TAJ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1847"/>
    <dbReference type="EnsemblBacteria" id="ADZ10069">
        <property type="protein sequence ID" value="ADZ10069"/>
        <property type="gene ID" value="Metbo_1847"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1847"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_3_2"/>
    <dbReference type="OMA" id="YRQFYFR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="183"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="472" mass="53247" checksum="A861F2EE6784179C" modified="2011-05-03" version="1">MKVALINPAQLDSKYKFMGVVAPPLGLAYMAAVLEENDVEVIIIDACALEMDLVSVGRQLREFSPDIIALTALTPTIAKALETAEYSKSVCKDSLIVMGGYHPSFNYKEILKYDFVDVVTIGEGEETLLDLTRSLEYDLPLTSVNGIAFDDVVTPPRQLIMDLDSLPLPARHLLPMDSYKLLNMDTKMSTMITSRGCPMQCSFCSSAALHGSKLRMRSVDKILDEMEYLVNEMGIETIAFMDDTFTISKKRVIELCEGIEERNIDVMWGCTSRVDSLNKELLRKMKKAGCITVFMGVESADQQMLDTVNKQTTIERIKDAFEVSRQEKIRTIASVVLGMPGDTHESIKNTINFVKELNPSYAIFSLATPYPGTRFYQQTLEKDLIKVKDWSKYTLISPILETVDCSLDELKKLQASAFKKFYLRPMYLIRQVIMDGPILVKTISGVMRNIMFPAKKNDDVPMKFQKRMVDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM3</accession>
    <name>F0TAM3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645446.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1874"/>
    <dbReference type="EnsemblBacteria" id="ADZ10095">
        <property type="protein sequence ID" value="ADZ10095"/>
        <property type="gene ID" value="Metbo_1874"/>
    </dbReference>
    <dbReference type="GeneID" id="10278332"/>
    <dbReference type="KEGG" id="mel:Metbo_1874"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="OMA" id="GNHDTML"/>
    <dbReference type="OrthoDB" id="105276at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="CHP00024_Pesterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF5">
        <property type="entry name" value="PTHR30337:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="245" mass="28651" checksum="015DAF1C216BF6C5" modified="2011-05-03" version="1">METNTIYNAEIADLGLIINDHLIISDIHIGYEGALNREGIMVPRFQYKKILERINEIIRKYEPKKVIVNGDLKHEFGRITSQEWKESKDFIEFLKTNFQDTILIKGNHDNFTKFIAEKTGLEVYESYELNEHVIMHGDKLPDETTLKNSKTIIIGHEHPCIGIRNGERLEKIKCYMKGSYQDKNMIVMPSFNFVTEGSDILHEKPLSPLLKNNKTDEFEVYGVENFEVLYFGRIKNILKVKDKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW7</accession>
    <name>F0TAW7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2070 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644344.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0742"/>
    <dbReference type="EnsemblBacteria" id="ADZ08993">
        <property type="protein sequence ID" value="ADZ08993"/>
        <property type="gene ID" value="Metbo_0742"/>
    </dbReference>
    <dbReference type="GeneID" id="10277189"/>
    <dbReference type="KEGG" id="mel:Metbo_0742"/>
    <dbReference type="eggNOG" id="arCOG04351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441213_0_0_2"/>
    <dbReference type="OMA" id="PSTHDFN"/>
    <dbReference type="OrthoDB" id="10400at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="9"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="607" mass="65765" checksum="9B59D2CFFF41AB31" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY4</accession>
    <name>F0TAY4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Shikimate kinase</fullName>
            <shortName evidence="12">SK</shortName>
            <ecNumber evidence="5 12">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">aroK</name>
        <name evidence="14" type="ordered locus">Metbo_1910</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="5 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645481.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1910"/>
    <dbReference type="EnsemblBacteria" id="ADZ10130">
        <property type="protein sequence ID" value="ADZ10130"/>
        <property type="gene ID" value="Metbo_1910"/>
    </dbReference>
    <dbReference type="GeneID" id="10278368"/>
    <dbReference type="KEGG" id="mel:Metbo_1910"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OMA" id="WDVLVWT"/>
    <dbReference type="OrthoDB" id="98200at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="PTHR20861:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="13">
        <location>
            <begin position="83"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="301" mass="31670" checksum="6D5EC4ADD64509CA" modified="2011-05-03" version="1">MKTIAQSPGSATIINAISTGSGSAFGIKLYVTAEVKLTESKSTINCSTDRNVDTTLMEKCVKKVLDKFKENFPEHEIDTGVSVKTASTLPVASGLSSSSATSNAVIMATHEALKTKYLDGSTEDYLNSHDLINMGVDASLEAGVTITGAFDDASASFFGGYTVTNNVNRKILKMDKLDEQNILVFMPNRKSLTAQSDVSRMKLISPQVKLAFEEALKGNLYDALTLNGILYSAALGFNPKMALDAIGAGARAAGLSGTGPSFVAVAGDGDIDNIADVWSSYPGEIIRTEVDNTGTRVIGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB16</accession>
    <name>F0TB16_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="4">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcm</name>
        <name evidence="5" type="ordered locus">Metbo_1942</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645513.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1942"/>
    <dbReference type="EnsemblBacteria" id="ADZ10162">
        <property type="protein sequence ID" value="ADZ10162"/>
        <property type="gene ID" value="Metbo_1942"/>
    </dbReference>
    <dbReference type="GeneID" id="10278402"/>
    <dbReference type="KEGG" id="mel:Metbo_1942"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OMA" id="TISAIHM"/>
    <dbReference type="OrthoDB" id="88476at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23947" checksum="1A97B85014248E90" modified="2011-05-03" version="1">MNKRRELVNTLRNMGYIKTDKVKKAMLNVPREEFMTPETRDLAYLDRPIPLKHGQTISAPHMVAMICEQLSLKPGMNVLEIGTGFGYNAAVVAEILGPEGHVYTIERIESLKERAEKNLIKTGFTNVAVIHGDGTAGYPEKAPYDRIYATASAPKIPDPLVDQLKVGGRLLAPIGEDPNYQELICILRGGKTEYQTFNLGGVVFVPMIGEHGWPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB27</accession>
    <name>F0TB27_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645524.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1953"/>
    <dbReference type="EnsemblBacteria" id="ADZ10173">
        <property type="protein sequence ID" value="ADZ10173"/>
        <property type="gene ID" value="Metbo_1953"/>
    </dbReference>
    <dbReference type="GeneID" id="10278413"/>
    <dbReference type="KEGG" id="mel:Metbo_1953"/>
    <dbReference type="eggNOG" id="arCOG00273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042632_1_0_2"/>
    <dbReference type="OMA" id="EPHSHWH"/>
    <dbReference type="OrthoDB" id="80550at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="174"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="312" mass="34286" checksum="9D8CF6B6D7DFFACB" modified="2011-05-03" version="1">MSSSSKAAVSNVKWGYLSLVAVTVLFGIWNPINKILLQDLDPLALSALIYSIAGIFLFLVRYSGINNHLMSMMDSNQEAETVFKTKDYMLIFITAVSGSVIAPLVYLSGLNNITAVNASLLMNVEVLFTIAFGIVLLKERFRKKELIGVFLIVLGTVLLATNGSSLEFTASEGLGSILIVVSTFFWSLDTILSKFLSFKRDLLMICATKCTVGGLILLTLSLMLHKNLTLPLDHIIYLLFAGVFIIGCTVVMILYSIRQIGSARTGSIFPFAALSGAIFSFIILKEPLTIMQLLYGMLMIVGVFIIYKFQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB73</accession>
    <name>F0TB73_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644375.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0773"/>
    <dbReference type="EnsemblBacteria" id="ADZ09024">
        <property type="protein sequence ID" value="ADZ09024"/>
        <property type="gene ID" value="Metbo_0773"/>
    </dbReference>
    <dbReference type="GeneID" id="10277221"/>
    <dbReference type="KEGG" id="mel:Metbo_0773"/>
    <dbReference type="eggNOG" id="arCOG04069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017233_1_0_2"/>
    <dbReference type="OMA" id="FPIHKDL"/>
    <dbReference type="OrthoDB" id="58391at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="PTHR12150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30811" checksum="2A5350A39AF59ADE" modified="2011-05-03" version="1">MEKKRLSIFVPASILSETKDLRIKTYKIGLIGRSAAIFRADRIVVYNDNSDEKEVKFISDVLTYMNTPQYLRKKVFPITRELRNVGILPPLRTPHHPTGELHEGDYRQGLTIKRTKKGTVVDIGADKFALCKEKLSVNKVLSFKVIKLGKEIILDPDVPDFYWGYKVLATNKDLYNSILMMKPGPDLVIGTSRYAPSITSVLDEVKTKLKGSKHTAILFGGPYSGLEELISDQNEKEIIDLEVNTVPEQGTKTVRTEEAVLATLSVFNLLLNTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB76</accession>
    <name>F0TB76_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl23</name>
        <name evidence="5" type="ordered locus">Metbo_0776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644378.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0776"/>
    <dbReference type="EnsemblBacteria" id="ADZ09027">
        <property type="protein sequence ID" value="ADZ09027"/>
        <property type="gene ID" value="Metbo_0776"/>
    </dbReference>
    <dbReference type="GeneID" id="10277224"/>
    <dbReference type="KEGG" id="mel:Metbo_0776"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OMA" id="MDPYKVI"/>
    <dbReference type="OrthoDB" id="113971at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_L23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_L23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9667" checksum="BBB6C0BB36466EDB" modified="2011-05-03" version="1">MDAYKVLIKPHLTEKSMNSIDQKNELTFVVLRTANKTQIKSAFESLYDVKVMRVNTQILNGQKLAYIKLSEDDSAEDIAVKMGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB79</accession>
    <name>F0TB79_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl22</name>
        <name evidence="7" type="ordered locus">Metbo_0779</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="4 6">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644381.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0779"/>
    <dbReference type="EnsemblBacteria" id="ADZ09030">
        <property type="protein sequence ID" value="ADZ09030"/>
        <property type="gene ID" value="Metbo_0779"/>
    </dbReference>
    <dbReference type="GeneID" id="10277227"/>
    <dbReference type="KEGG" id="mel:Metbo_0779"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OMA" id="ANAEYKG"/>
    <dbReference type="OrthoDB" id="103467at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_L22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_L22/L17_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="PTHR11593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17190" checksum="50D71ACCB34CD158" modified="2011-05-03" version="1">MAKIQYAYQDEYDGKTAKAAGKALKISPKHSVEICRTIRGMYLDDAREFLEAVIRKETPVPFKRHNKKVGHRKGLKGWPTGRYPVKAATQILQVLDNAEANAEYKGLETEDLKIVHISSHRGYIVRGWTARAFGRASPFNTPTTHIQVVLGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD8</accession>
    <name>F0TBD8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645558.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1987"/>
    <dbReference type="EnsemblBacteria" id="ADZ10207">
        <property type="protein sequence ID" value="ADZ10207"/>
        <property type="gene ID" value="Metbo_1987"/>
    </dbReference>
    <dbReference type="GeneID" id="10278447"/>
    <dbReference type="KEGG" id="mel:Metbo_1987"/>
    <dbReference type="eggNOG" id="arCOG01078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_19_3_2"/>
    <dbReference type="OMA" id="NPGKWEL"/>
    <dbReference type="OrthoDB" id="116493at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="18858" checksum="F1A8AFA5CFA38647" modified="2011-05-03" version="1">MINHVYGLAVRVLLVDENGKVLILKRSTDSKTNPGKWELPGGKVNQDESFDHALLREVYEETGLKIELGHVVGASEQNLHIIRAVHIIMSGKIVEGELTLSSEHEGYAWVLMETLGDYELADWLEDYLNKSTTFHFKDPEEVDKTDNSFDPLIKSIKTTFDKMSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH0</accession>
    <name>F0TBH0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2020"/>
    <dbReference type="EnsemblBacteria" id="ADZ10239">
        <property type="protein sequence ID" value="ADZ10239"/>
        <property type="gene ID" value="Metbo_2020"/>
    </dbReference>
    <dbReference type="GeneID" id="10278480"/>
    <dbReference type="KEGG" id="mel:Metbo_2020"/>
    <dbReference type="eggNOG" id="arCOG08127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168019_0_0_2"/>
    <dbReference type="OMA" id="DINGPPE"/>
    <dbReference type="OrthoDB" id="115155at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="117" mass="12777" checksum="0B8643DCA4CA4FAF" modified="2011-05-03" version="1">MDINGPPENGSFNRSGAERPPLDDPSLAIFAAVVCAANIGLLLILLFTYVTSYRKLKSQFTLGLIIFATLLIVQNILFMFFLLAREGFHGAGMGFPVLSLSIIEFGALLVLLKTTWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH6</accession>
    <name>F0TBH6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DZANK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2026</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645596.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2026"/>
    <dbReference type="EnsemblBacteria" id="ADZ10245">
        <property type="protein sequence ID" value="ADZ10245"/>
        <property type="gene ID" value="Metbo_2026"/>
    </dbReference>
    <dbReference type="GeneID" id="10278487"/>
    <dbReference type="KEGG" id="mel:Metbo_2026"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056069_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="5">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13323" checksum="28EECF33F8E727E5" modified="2011-05-03" version="1">MIKIRKCNIIQDEKGAFFVFGPLWFVILVMIVGFIATLIKNNNGKNNPEKVYDYNPNQIVNPLKSRNICPKCGQNNSKSAKFCNKCGSNLLPKNKSCSKCGSINQTNANFCQDCGNRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL8</accession>
    <name>F0TBL8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0845"/>
    <dbReference type="EnsemblBacteria" id="ADZ09095">
        <property type="protein sequence ID" value="ADZ09095"/>
        <property type="gene ID" value="Metbo_0845"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0845"/>
    <dbReference type="eggNOG" id="arCOG07350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073290_2_0_2"/>
    <dbReference type="OMA" id="EDLVAYP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="296" mass="34213" checksum="FAF7F524164C531E" modified="2011-05-03" version="1">MGSVELDKRFKYEAFGLNILSDFYLPHLISTNFDKPDVKIINSKFDLDLKQVNQSGPNFYIDKQYIYRFWDFVGKFKINNNTIIVDPKLDVDQNILNNFILGTIFATLLRLRGLFVLHASAVNIHGFAIAFSGFKGYGKSTTAMAFYNRGYPIISDDYIAIDFHNEIPMISQGFSCLKLSLNSAQYLHKNKVINSFEKKRYFSVPQAYVTKKLPLKKIYFIERNEYSKIYDITGQNAFVELIKNTFGINMFKNTELAINFLQCNKIFQTVNLSKLIISDNLNDLDKLVKLVEEDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN1</accession>
    <name>F0TBN1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644459.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0858"/>
    <dbReference type="EnsemblBacteria" id="ADZ09108">
        <property type="protein sequence ID" value="ADZ09108"/>
        <property type="gene ID" value="Metbo_0858"/>
    </dbReference>
    <dbReference type="GeneID" id="10277307"/>
    <dbReference type="KEGG" id="mel:Metbo_0858"/>
    <dbReference type="eggNOG" id="arCOG03188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_447335_0_0_2"/>
    <dbReference type="OMA" id="ILTHQVS"/>
    <dbReference type="OrthoDB" id="5877at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="610" mass="69763" checksum="AF9BB82453A8A135" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN3</accession>
    <name>F0TBN3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644461.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0860"/>
    <dbReference type="EnsemblBacteria" id="ADZ09110">
        <property type="protein sequence ID" value="ADZ09110"/>
        <property type="gene ID" value="Metbo_0860"/>
    </dbReference>
    <dbReference type="GeneID" id="10277309"/>
    <dbReference type="KEGG" id="mel:Metbo_0860"/>
    <dbReference type="eggNOG" id="arCOG02878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_3_0_2"/>
    <dbReference type="OrthoDB" id="98160at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="62"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="266"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="403" mass="47993" checksum="704ED45DCE675688" modified="2011-05-03" version="1">MFKIPVPVIMYHSVGIPCNKWHYNFLTCPHKTFENQLKWLRKKNFNSISLNQLYESMKKGTDLPKNPFLITFDEGYLDNWTYAYPLLKKYGFKATIYVNPEFISNNNVHENLENVWNGDTNFDNIENVGFLSWKEIRIMEEDNVIDIQSHTLTHTSYPISPKIIDFRHPKDLYGWMSWNDNIDKKPYLQIDNENFINFGQPVYENERSIGAKIYYPDVNLDKILIDYVKENGEKEFFESKNWKSLLLNIANEYRENNLFEEIYENEKQRQDRIVYELKKSKEIIEKKLNKKVEFLCWPGGVTNLESLRKASELGYKSSTAGKDIKYERKMMKNVYGEDPSRINRVGATIYWDGYEGFGSKVKYFDGYTLIKFLHKFQNRNKFFVYPVLGSVYLATKFFYNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ6</accession>
    <name>F0TBQ6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0884</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644484.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0884"/>
    <dbReference type="EnsemblBacteria" id="ADZ09133">
        <property type="protein sequence ID" value="ADZ09133"/>
        <property type="gene ID" value="Metbo_0884"/>
    </dbReference>
    <dbReference type="GeneID" id="10277333"/>
    <dbReference type="KEGG" id="mel:Metbo_0884"/>
    <dbReference type="HOGENOM" id="CLU_867707_0_0_2"/>
    <dbReference type="OrthoDB" id="114753at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="320" mass="36359" checksum="18DD31B3F1A8189E" modified="2011-05-03" version="1">MNELFKSFVMTYRASRSLKYGGYFITILPFIAILYYNGFINSPWALLYLLPLVPAFAGGFMWNAIGDADKDPKSKNPVTQGLISVKFLYVTIAVLYLLSILFLLLIYTSFASLMALLLLLLLAAIYSGFKLRMKESILGPVVASFGFFVLPSIMLLAEFNYFSLGTVTLLVGLFVIYFAHEIKHTIIEYDLDLSFDCRTFAVIVGKKNANIIEYASIILGFIFLTASIYYLLPNSYMISNYYIPTVYVMGVFIIFFALSLFSTLFYGFKTQFDQKGDVILNMLPYIASRTCYITIGLFLLNLPMLIIILALWLLFTDKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR2</accession>
    <name>F0TBR2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644490.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0890"/>
    <dbReference type="EnsemblBacteria" id="ADZ09139">
        <property type="protein sequence ID" value="ADZ09139"/>
        <property type="gene ID" value="Metbo_0890"/>
    </dbReference>
    <dbReference type="GeneID" id="10277339"/>
    <dbReference type="KEGG" id="mel:Metbo_0890"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_703243_0_0_2"/>
    <dbReference type="OrthoDB" id="106297at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="392" mass="44840" checksum="7EBA3B96BC34DF42" modified="2011-05-03" version="1">MKIAIIAPMDPKTGISNYSETLAIELIKLGQDVDVVSPENSCNTYLTSQERMNHVKPQDYSVNDYDVTHFQLANSPLHEFQLHILLDNNEDLIKNDSIVTTVHDARNFDVFQYKCSKCRSYGFNSFRSKLSFPHDLVDRGFQRVSNVLLFHNNSAITEYKDRYKLDNMKFKRVLHPAYRIPGQNIINKTNNETKTVLAPGYISPYKGQDILIKAVAGIDLDFKLVFMGNILDPDYGTYLNQLVHENELEDKVEFLGFVTSEEFIYNIDNAEAILVPRLTSPWLLNKPFFRIRRSMGLDTLINQSTSGVLTKALASGKPVICSQNKGFADYVQEDMGLMCDDNVESWSNAIKYMLVNPNKVKEMSINSKRFADNVLDPAKIAEEHLNIYREYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR8</accession>
    <name>F0TBR8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0896</name>
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    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ09145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0896"/>
    <dbReference type="EnsemblBacteria" id="ADZ09145">
        <property type="protein sequence ID" value="ADZ09145"/>
        <property type="gene ID" value="Metbo_0896"/>
    </dbReference>
    <dbReference type="GeneID" id="10277345"/>
    <dbReference type="KEGG" id="mel:Metbo_0896"/>
    <dbReference type="eggNOG" id="arCOG02859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709134_0_0_2"/>
    <dbReference type="OMA" id="MDINYTI"/>
    <dbReference type="OrthoDB" id="139386at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17758" checksum="69CED8D0673096A0" modified="2011-05-03" version="1" precursor="true">MIKLVKDRRFIFFGLLIVLIILSVYFASNYQNHLENPNTSIIVKNYPLGETVAVSGVVSDVHDGGFNLSDEYHGMDINYTITSGEHVSKGDQVEVLGILQPSYRVNATKILVITSFEYSFMLLRSALVALIFLFFFFRYWEFDLKKLEFRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT2</accession>
    <name>F0TBT2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0910</name>
    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
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    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Metbo_0910"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="64322at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="107"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09159.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="640" mass="75736" checksum="812F8AB4E73F2986" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW7</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="15157" checksum="7FE8C4842E0406E2" modified="2011-05-03" version="1">MEYSFILKGHPNVTSRHKTTLEFTKDPEIGLKADCIVGVGSGVSMENFPTGMIDAIQDENTKITVKLETENAKDEIHGFGHPDLTLDHPTDMVVRKSEFKCSRTLMINADKAACDLKLKLVRDLADSKQLKVTIIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY2</accession>
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    <gene>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2105"/>
    <dbReference type="EnsemblBacteria" id="ADZ10324">
        <property type="protein sequence ID" value="ADZ10324"/>
        <property type="gene ID" value="Metbo_2105"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_2"/>
    <dbReference type="OMA" id="KQSDMKA"/>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
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    <dbReference type="InterPro" id="IPR045851">
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    </dbReference>
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        <property type="entry name" value="AMP-binding_CS"/>
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    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="453"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="552" mass="62977" checksum="DE22F6AF3D7E2A99" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC60</accession>
    <name>F0TC60_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644562.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0964"/>
    <dbReference type="EnsemblBacteria" id="ADZ09211">
        <property type="protein sequence ID" value="ADZ09211"/>
        <property type="gene ID" value="Metbo_0964"/>
    </dbReference>
    <dbReference type="GeneID" id="10277413"/>
    <dbReference type="KEGG" id="mel:Metbo_0964"/>
    <dbReference type="eggNOG" id="arCOG01153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063034_1_0_2"/>
    <dbReference type="OMA" id="CGHCHIP"/>
    <dbReference type="OrthoDB" id="49505at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="4"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="28216" checksum="3F0BA37192B94FD8" modified="2011-05-03" version="1">MPFIAQISDLHVGSLNFQENLLDKAIDEINSVDPDVTIVTGDITENGYYLEYERVVPFLDRIKSPILVVPGNHDARHVGDECFEDLIKNRYGTLEDKKHGLKVIGLDSSEPDLDYGRIGRSQQSWMENELRIADEENLYKIIALHHHIIPVPKTGRERNVLSDAGDILQSLMKGKADLVLSGHKHMPHVWMMEDTTFITAGTVSSLRLRGKALSSYNTIDIEDEFIEIILNRADGTRKCLAKYENTCLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC80</accession>
    <name>F0TC80_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="4">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="4">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="4">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mobA</name>
        <name evidence="6" type="ordered locus">Metbo_0984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MobA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0984"/>
    <dbReference type="EnsemblBacteria" id="ADZ09231">
        <property type="protein sequence ID" value="ADZ09231"/>
        <property type="gene ID" value="Metbo_0984"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0984"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_2_2"/>
    <dbReference type="OMA" id="HENGYIE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="22936" checksum="9A27538A3292960E" modified="2011-05-03" version="1">MKSVIVLCGGLSTRMGQDKGSMNYHGKPMIVHVLESLNQVADEVVLVLRNEVQYNKYKNLLSKNAFLESSNFKILKDTIKDKGPLGGIYTGISAINSDKALIAPCDSPFISKELVTQLFEISKNYPDFESFVPIWSDGNMEPLHSIYPVRSKEIIEDLLLKDQKNVKALIKRLNVKYIKIDEFDLPKKSFLNLNSPQDISKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC84</accession>
    <name>F0TC84_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="3">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiT</name>
        <name evidence="4" type="ordered locus">Metbo_0988</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + CO2 + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644586.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0988"/>
    <dbReference type="EnsemblBacteria" id="ADZ09235">
        <property type="protein sequence ID" value="ADZ09235"/>
        <property type="gene ID" value="Metbo_0988"/>
    </dbReference>
    <dbReference type="GeneID" id="10277437"/>
    <dbReference type="KEGG" id="mel:Metbo_0988"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="OMA" id="RCKFVYA"/>
    <dbReference type="OrthoDB" id="82864at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="3">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="187" mass="20482" checksum="5062EDF4F90EC641" modified="2011-05-03" version="1">MIKDSDFIKNPKVPGPTKEEIRCILLCKSEVSEEDVVVDIGCGTGGLTVEFAKKAKKVYSIDLNPLATQTTQENVNKHQVKNKVEVIQADGLEALEDLEEFDVLMIGGSSGKLPQLIKKGYKKLNKNGRILVTSILLETATEAISTFKELSLKSEVVNIMISKGTPGQRGTMMYSNNPITVVITEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE7</accession>
    <name>F0TCE7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645765.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2200"/>
    <dbReference type="EnsemblBacteria" id="ADZ10414">
        <property type="protein sequence ID" value="ADZ10414"/>
        <property type="gene ID" value="Metbo_2200"/>
    </dbReference>
    <dbReference type="GeneID" id="10278663"/>
    <dbReference type="KEGG" id="mel:Metbo_2200"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117563_0_0_2"/>
    <dbReference type="OMA" id="NEWWGGR"/>
    <dbReference type="OrthoDB" id="116082at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017255">
        <property type="entry name" value="AcTrfase_GNAT_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037663">
        <property type="entry name" value="Acetyltransf_GNAT_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18321" checksum="D9B61E8ECB8C694D" modified="2011-05-03" version="1">MDLDIRNVVTDDFMGIARLAENCGHMSTMPNAVYHLFTRHFKKTSFVVEDNSNHKILGFLLGFISQDDPEESYIHLLCLDSELRGGGIGKALMNQFAETVSKNGCKKIKLVTKPHNKISINFYLKMGFKSCKSSETFESEGLNVYKDFDGPDEDKIIFIKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH8</accession>
    <name>F0TCH8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenylacetate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644606.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1008"/>
    <dbReference type="EnsemblBacteria" id="ADZ09255">
        <property type="protein sequence ID" value="ADZ09255"/>
        <property type="gene ID" value="Metbo_1008"/>
    </dbReference>
    <dbReference type="GeneID" id="10277457"/>
    <dbReference type="KEGG" id="mel:Metbo_1008"/>
    <dbReference type="eggNOG" id="arCOG02620">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_1_2"/>
    <dbReference type="OMA" id="IHCTPSY"/>
    <dbReference type="OrthoDB" id="17209at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047475">
        <property type="term" value="F:phenylacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05913">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011880">
        <property type="entry name" value="PA_CoA_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006444">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="78"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C_2" evidence="2">
        <location>
            <begin position="334"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="48583" checksum="4524C1B7A0992FB4" modified="2011-05-03" version="1">MIWNEKAECMSEDDLKELQLQRLQAVVKMAYEKVPYYNKRFTDLKIEPEDIETLEDIEKLPFTSKTDLRDAYPFGMFAVDTEDIVEVHTTSGTTGKPTVSGYTEADLDLWGEVMARALTMTGVKKNDKVQNSYGYGLFTGGMGVHYGGQKIGATVIPISAGNTTRQLEIMQDFGTTVLTCTPSYGLYLAEVAEKEGIDPSKLKLKAGCFGAEMWTEEMRNKLEERLNISAQNIYGLTEVIGPGVAMECPEKNGLHIFEDHFYPEIINPKTLNQLPEGETGELVLTTLTREGMPVIRFRTKDITALRRGVCECGRTQVKMDRITGRSDDMLKVRGVIVFPSQIEKALLKIEGLEPQYQIIVTRPKHLDELEVQVETSPALFSDEVKHIEEVKKDIENRIHSEIGLRVNISLVEPQSLPRSEGKAVRVVDKRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI1</accession>
    <name>F0TCI1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase</fullName>
            <ecNumber evidence="2">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.4.7" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644609.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1011"/>
    <dbReference type="EnsemblBacteria" id="ADZ09258">
        <property type="protein sequence ID" value="ADZ09258"/>
        <property type="gene ID" value="Metbo_1011"/>
    </dbReference>
    <dbReference type="GeneID" id="10277460"/>
    <dbReference type="KEGG" id="mel:Metbo_1011"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_0_2"/>
    <dbReference type="OMA" id="DNRHTHG"/>
    <dbReference type="OrthoDB" id="64383at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="1">
        <location>
            <begin position="10"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="26771" checksum="42682C036AFD7367" modified="2011-05-03" version="1">MIALSIAGYDPSGGAGILNDVKTFQAMGIYGTGVVTVLTAQNPERVEAIEPVSTEFIEKQLETLLNVYPIKYCKTGMLYNKENLKLVGAKTKEHNLKVVVDPVIVAGCGAELSVDGYTKELKKYLLPNATLTTPNIYEAELLSGQKIESIDDAVEAAVKIGKICDVVITGGNLNGSNVVFDGTLKVIENELIGDVEVHGTGCSFSAAVTVGLLKNNSLKRSVEDAVEFVKHGVEHGKWGTLDQFHGKRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI6</accession>
    <name>F0TCI6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644614.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1016"/>
    <dbReference type="EnsemblBacteria" id="ADZ09263">
        <property type="protein sequence ID" value="ADZ09263"/>
        <property type="gene ID" value="Metbo_1016"/>
    </dbReference>
    <dbReference type="GeneID" id="10277465"/>
    <dbReference type="KEGG" id="mel:Metbo_1016"/>
    <dbReference type="eggNOG" id="arCOG07495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203781_1_0_2"/>
    <dbReference type="OMA" id="KRCYYAP"/>
    <dbReference type="OrthoDB" id="126490at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="6940" checksum="983E87944F1601F9" modified="2011-05-03" version="1">MDNKEKVIKTFKESEEPLNATKVSQLSGVEKKEVDKIMKELKKDETIYSPKRCYYALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM8</accession>
    <name>F0TCM8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative ABC-type transport system, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1058</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1058"/>
    <dbReference type="EnsemblBacteria" id="ADZ09305">
        <property type="protein sequence ID" value="ADZ09305"/>
        <property type="gene ID" value="Metbo_1058"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1058"/>
    <dbReference type="eggNOG" id="arCOG01462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091276_0_0_2"/>
    <dbReference type="OMA" id="VPIGVVW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="242" mass="27416" checksum="621D048B8AD6CE06" modified="2011-05-03" version="1">MNFSKSWVIANKDFSIFMKKKRILYTLIILPLIFSIILPLSIRSLTNETDPADVIALINAFSYFYVILVYIQSTTLASYSILGEKIEQSLEPLLATPTTESELLLGKTVASFLPSVAIIYANSVIFMVLIDLFTKTSFGYLFYPNWTMAFILLVVVPLSSIFSIQLNVIISSRVNDVRTANQLGFLMFLPFMGLYIPLVTNSLSLNIVNLTVLSFTILLIDIILFYISKSTFNRDKILTKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN1</accession>
    <name>F0TCN1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09308">
        <property type="protein sequence ID" value="ADZ09308"/>
        <property type="gene ID" value="Metbo_1061"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1061"/>
    <dbReference type="HOGENOM" id="CLU_2748204_0_0_2"/>
    <dbReference type="OrthoDB" id="131634at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="70" mass="8159" checksum="94C4AB8A92339EB5" modified="2011-05-03" version="1">MSKREHETLFERKTNVGPFSNCADIKPQKEGFNDAICEVCEKVFKTNENVYICPECIKKEKYKGQVDLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR7</accession>
    <name>F0TCR7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645808.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2243"/>
    <dbReference type="EnsemblBacteria" id="ADZ10457">
        <property type="protein sequence ID" value="ADZ10457"/>
        <property type="gene ID" value="Metbo_2243"/>
    </dbReference>
    <dbReference type="GeneID" id="10278709"/>
    <dbReference type="KEGG" id="mel:Metbo_2243"/>
    <dbReference type="eggNOG" id="arCOG03228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072513_0_0_2"/>
    <dbReference type="OMA" id="HAESRMV"/>
    <dbReference type="OrthoDB" id="45551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="28085" checksum="8086108C5D31BE20" modified="2011-05-03" version="1">MIEKVTVLGGYNKQGEEESVKKVVIKKGEIVGVVGPTGSGKSSLIGDIEQLAQKDTFSKRKILVNDEEPSYDDRTNPRKKMVAQLSQNMNFLADMSVGDFLNLHAKCRGASSKCADAVIKLANTLTGEPIKEEHDLTILSGGQSRALMVADVAIISDSPIVLIDEIENAGIRKHDALNVLSGHGKIVMVVTHDPVLALMTDRRIVMRSGGMEKVISTTESEKSISKKLNRIDDLLLNLRDKVRNGDVIEDINLDGVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS5</accession>
    <name>F0TCS5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl1</name>
        <name evidence="6" type="ordered locus">Metbo_2251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Probably involved in E site tRNA release (By similarity). Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645816.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2251"/>
    <dbReference type="EnsemblBacteria" id="ADZ10465">
        <property type="protein sequence ID" value="ADZ10465"/>
        <property type="gene ID" value="Metbo_2251"/>
    </dbReference>
    <dbReference type="GeneID" id="10278717"/>
    <dbReference type="KEGG" id="mel:Metbo_2251"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="70269at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_L1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_L1_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="23493" checksum="FD5CAD5349A3A5C3" modified="2011-05-03" version="1">MKEEILEAVKKAKEDSKPRNFTQSIDVVITIKDLDVKKPENRIDEEVFLPNGRGKDVSVVFIADGELALQAENAGASLVITKEGLESFGKDRKAAKKVANQNDFFVAQADFMPLVGRFLGPVLGPRKKMPKPVPASAKPEPLMERLKNTAKVRIKDQPVIQAIVGSQEMDDEQIAENIEAILQILDQKLEKGRSQIKSMYIKTTMGPVARVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCW2</accession>
    <name>F0TCW2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645853.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2288"/>
    <dbReference type="EnsemblBacteria" id="ADZ10502">
        <property type="protein sequence ID" value="ADZ10502"/>
        <property type="gene ID" value="Metbo_2288"/>
    </dbReference>
    <dbReference type="GeneID" id="10278754"/>
    <dbReference type="KEGG" id="mel:Metbo_2288"/>
    <dbReference type="eggNOG" id="arCOG00519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105338_0_0_2"/>
    <dbReference type="OMA" id="CRGWDTN"/>
    <dbReference type="OrthoDB" id="95849at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12641">
        <property type="entry name" value="Flavodoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="16445" checksum="5D327234451D05AE" modified="2011-05-03" version="1">MKVLIIYNSVHRGNTEKIARAMADSIDADILSYSEVDGYNVLDYDLVGFGSGIYYGKPGKDFVEFIDSLPIVKNKKAFVFTTSGKGDSNYSEKLAAKVSENGFKIVGEFSCKAFDAWGPLKLVGGINKGRPNSDDLKSAHNFICQLVGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S8</accession>
    <name>F0T5S8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644672.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09321">
        <property type="protein sequence ID" value="ADZ09321"/>
        <property type="gene ID" value="Metbo_1076"/>
    </dbReference>
    <dbReference type="GeneID" id="10277525"/>
    <dbReference type="KEGG" id="mel:Metbo_1076"/>
    <dbReference type="HOGENOM" id="CLU_1830607_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="15754" checksum="71CA748163B2534F" modified="2011-05-03" version="1" precursor="true">MYNEQKKSIWIIIPVILWTLILVSNISTVAAVNTEMDPISPITCKQGDVVGITAQLWIKGEFWNNPLIGEEVTFCIYDGNLKILFYETTKTNWINGKAGVEVNTKNFEPENYILSVYYFGNTGLLNFDPCGTDSKFSVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U6</accession>
    <name>F0T5U6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum-pterin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644690.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09339">
        <property type="protein sequence ID" value="ADZ09339"/>
        <property type="gene ID" value="Metbo_1094"/>
    </dbReference>
    <dbReference type="GeneID" id="10277543"/>
    <dbReference type="KEGG" id="mel:Metbo_1094"/>
    <dbReference type="eggNOG" id="arCOG00228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118993_1_2_2"/>
    <dbReference type="OMA" id="MIISARN"/>
    <dbReference type="OrthoDB" id="124036at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00638">
        <property type="entry name" value="Mop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mop" evidence="3">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="7398" checksum="FF607B3D98E27802" modified="2011-05-03" version="1">MKISARNVVEGTVEGVEEGAVMANVKIKIESPDVITAIITKESIKDLDIKVGDTVKAIIKSTEVMIAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X7</accession>
    <name>F0T5X7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644721.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09370">
        <property type="protein sequence ID" value="ADZ09370"/>
        <property type="gene ID" value="Metbo_1125"/>
    </dbReference>
    <dbReference type="GeneID" id="10277575"/>
    <dbReference type="KEGG" id="mel:Metbo_1125"/>
    <dbReference type="eggNOG" id="arCOG10898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147142_0_0_2"/>
    <dbReference type="OrthoDB" id="142751at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="11594" checksum="CB58328BA58934C7" modified="2011-05-03" version="1">MVEIKWMPVIIGLVIAIILSIILGLFLGSWGDIIGFLIATIYVGYSVGGDYMNGVVHGALVGIVAAIMVGILAILGLSPILGAVAVAGNMSALILGIIVDGIIGAIGGVIGVLIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T626</accession>
    <name>F0T626_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PAS sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645884.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2319"/>
    <dbReference type="EnsemblBacteria" id="ADZ10533">
        <property type="protein sequence ID" value="ADZ10533"/>
        <property type="gene ID" value="Metbo_2319"/>
    </dbReference>
    <dbReference type="GeneID" id="10278785"/>
    <dbReference type="KEGG" id="mel:Metbo_2319"/>
    <dbReference type="eggNOG" id="arCOG02393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835785_0_0_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="213"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="266"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="332" mass="38257" checksum="7EB22CD7EEBF5F15" modified="2011-05-03" version="1">MNKKKFGRRLKISAIKIFLVCNVDSVVKDLETTIESLGHSVTMVKRLQKLELKPDIILVDSSLNKKFLEATISMINKLDLPFIYISTPEEGFNQEVVDQVNYFGTIIKPVDPELLKTSIEFALQKHLKEKELKDRINKLEKRESRYHALIESSFDSVYIMSPDWGEMKHLEGRNFLNSTLNPSKSWINTYIPPEEREKVLTTINNAIKDKTKFELEHKVIAADGTIGQTLSRAVPVLDTNGEIIEWIGTAKDITKRTNIEWELTETKNMLESVIESIPDAVFVSDDEGNFLKFNKAFATYHKFKSKKDVKKTLNEYPDFLEVYGDEKYIIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A0</accession>
    <name>F0T6A0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murT</name>
        <name evidence="4" type="ordered locus">Metbo_1171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:59488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with GatD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurT subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1171"/>
    <dbReference type="EnsemblBacteria" id="ADZ09415">
        <property type="protein sequence ID" value="ADZ09415"/>
        <property type="gene ID" value="Metbo_1171"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1171"/>
    <dbReference type="eggNOG" id="arCOG02821">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041534_0_0_2"/>
    <dbReference type="OMA" id="WLWDVDY"/>
    <dbReference type="OrthoDB" id="43066at2157"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02214">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043703">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013564">
        <property type="entry name" value="MurT_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08353">
        <property type="entry name" value="MurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="54"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="MurT_C" evidence="3">
        <location>
            <begin position="321"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="463" mass="52377" checksum="0F0B3D5004DA95F1" modified="2011-05-03" version="1">MFDIHPSVIAGRLTIFGLKLFGMKGTALPGKVAMKLDPDILKLIDSRCKKKLIITGTNGKTTTNNLINHILGENYPNSLSNLMGANMPQGIASAFLNNLKEEYDWGIFEVDEGSFEEIVKHIKPDYVLITNFFRDQLDRFGEIENTAKMVYDAIKPLDTTLILNADDPLVVKFKDLNKPSIYYGVQKTHFSSLNQSVVESRFCPECNEGLEYEYYNYGQLGKYQCDNCGFKNPDYQYKISSIEYINNSYEFEIVNSQGDSSTINFGYDGIYNAYNCCAAVAFARETGLEMDTIKSRIGNFDYKLGRMENFSHKSKIIKIVLVKNPIGLTEVLNSITNDERKKSLLFILNDNPADGTDVSWIWDADVETVQNIKNLENIFFSGTRADDMALRLKYSGLTVKPELINTNIEDSINDAVNSAVEIVYILPTYTAVYNTREIVINLINSNNPKIQRFREVLRSKLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E6</accession>
    <name>F0T6E6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644812.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1219"/>
    <dbReference type="EnsemblBacteria" id="ADZ09461">
        <property type="protein sequence ID" value="ADZ09461"/>
        <property type="gene ID" value="Metbo_1219"/>
    </dbReference>
    <dbReference type="GeneID" id="10277670"/>
    <dbReference type="KEGG" id="mel:Metbo_1219"/>
    <dbReference type="eggNOG" id="arCOG03230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173284_0_0_2"/>
    <dbReference type="OrthoDB" id="124622at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025698">
        <property type="entry name" value="2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13239">
        <property type="entry name" value="2TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="2TM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10767" checksum="4A42D56614F4DAA8" modified="2011-05-03" version="1">MDENYVQAKNRMHQLKAFYKNLISYVVVMIFLVVINYFTSPNFWWVLFPALIWGIFVIIQGVSVYSKRGLFSKEWEDKKIQEYMDENED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G3</accession>
    <name>F0T6G3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645948.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2384"/>
    <dbReference type="EnsemblBacteria" id="ADZ10597">
        <property type="protein sequence ID" value="ADZ10597"/>
        <property type="gene ID" value="Metbo_2384"/>
    </dbReference>
    <dbReference type="GeneID" id="10278850"/>
    <dbReference type="KEGG" id="mel:Metbo_2384"/>
    <dbReference type="eggNOG" id="arCOG02408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100862_1_1_2"/>
    <dbReference type="OMA" id="MLRKEFC"/>
    <dbReference type="OrthoDB" id="88507at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00288">
        <property type="entry name" value="TIGR00288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="165" mass="18434" checksum="6CEB3EE550B89D17" modified="2011-05-03" version="1">MHRFEKLSSFKDYIPLIRENSQGKNIGLLVDGPNMLRKEFDCDLEIVRDLMLEHGNVKVGKVFLNQYASDKLIEAVVNQGFSPMIVSGETDVQMAIEAFELIHNPNIDIIALMTRDVDFFPLINVAKENGKQTIVIGAEPGFSAALKNSADDTITLKNSHRPHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I3</accession>
    <name>F0T6I3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645968.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2404"/>
    <dbReference type="EnsemblBacteria" id="ADZ10617">
        <property type="protein sequence ID" value="ADZ10617"/>
        <property type="gene ID" value="Metbo_2404"/>
    </dbReference>
    <dbReference type="GeneID" id="10278876"/>
    <dbReference type="KEGG" id="mel:Metbo_2404"/>
    <dbReference type="eggNOG" id="arCOG04854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772958_0_0_2"/>
    <dbReference type="OMA" id="SHHEFTH"/>
    <dbReference type="OrthoDB" id="69327at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="360" mass="39941" checksum="A95D8E6E942F05C6" modified="2011-05-03" version="1">MLIAENHLQFIIEIAILIHAAVLLMFNIIIIPLSLVLFLSMVLTIIIAALFGIDTIFLFLPFVSHHEFTHPFGPLAVFAWVTFFSSASLLTELEIRSASIKTLAIILFFIIAVAGGMMHRSFLLLWLLGWFIGTLLISRSFKRTSQITLKRVFAVIAAGLAGFGLLEVLSRILNFTILSPLLRISRVEEFAMPSLVTVINNTTLWGHVPGSCYWGAKCLGGSDGYLTLPMNFVNMFTLPFPLFAGVLVVKKDAIDYMLPGIFGVAFDFGYLGLFALMTWVMIVTVTGLYILREYRSKRLGGSRNYIGREALLIGALTAFISQSIIGLFLFNRSFNGAAMIIYILLSALVVAHIMSIKRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L9</accession>
    <name>F0T6L9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker domain 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646004.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2440"/>
    <dbReference type="EnsemblBacteria" id="ADZ10653">
        <property type="protein sequence ID" value="ADZ10653"/>
        <property type="gene ID" value="Metbo_2440"/>
    </dbReference>
    <dbReference type="GeneID" id="10278912"/>
    <dbReference type="KEGG" id="mel:Metbo_2440"/>
    <dbReference type="eggNOG" id="arCOG04853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727260_0_0_2"/>
    <dbReference type="OMA" id="PCPFRDS"/>
    <dbReference type="OrthoDB" id="96489at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017671">
        <property type="entry name" value="Methan_mark_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03277">
        <property type="entry name" value="methan_mark_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="158" mass="17714" checksum="F7B314BF4680F6C0" modified="2011-05-03" version="1">MAWEDSPSHVCRGGDKRALSFCCPPVKPCPVIYALEDAELTAQKYVEIKEKFAEETKLGQGEGTCFGSLVWCCKPSKPCPLRDMVMRRIDMSVDEYMDLKKQLSEQLVGQPESNTKESVVALAETFNVTGEEAFKVLKECDNDVKKAAKLLKMKDLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N8</accession>
    <name>F0T6N8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CutA1 divalent ion tolerance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643622.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0018"/>
    <dbReference type="EnsemblBacteria" id="ADZ08271">
        <property type="protein sequence ID" value="ADZ08271"/>
        <property type="gene ID" value="Metbo_0018"/>
    </dbReference>
    <dbReference type="GeneID" id="10276440"/>
    <dbReference type="KEGG" id="mel:Metbo_0018"/>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
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        <property type="entry name" value="N-reg_PII-like_a/b"/>
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    </dbReference>
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        <property type="entry name" value="PTHR23419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="12301" checksum="B9180339C78110FC" modified="2011-05-03" version="1">MYSSIYVTTSDISESRKIARVLVQERLAACVNIVPAIESIYRWNGEIEEDSESLIFIKTRSDLVENVIKRVEEIHSYDTPCVLELSIKRGSKKYFKWLDTEVDKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T732</accession>
    <name>F0T732_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-methyl-2-oxobutanoate dehydrogenase (Ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.7" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646017.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2453"/>
    <dbReference type="EnsemblBacteria" id="ADZ10666">
        <property type="protein sequence ID" value="ADZ10666"/>
        <property type="gene ID" value="Metbo_2453"/>
    </dbReference>
    <dbReference type="GeneID" id="10278925"/>
    <dbReference type="KEGG" id="mel:Metbo_2453"/>
    <dbReference type="eggNOG" id="arCOG01607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_0_2"/>
    <dbReference type="OMA" id="EAAINMV"/>
    <dbReference type="OrthoDB" id="34838at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043807">
        <property type="term" value="F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="246"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="38197" checksum="4A73B2481266E9F8" modified="2011-05-03" version="1">MATQLIKGNTAVIIGAMYAGCDCYFGYPITPATEILHEASKYFPMVGRKFVQAESEEAAINMVYGASAAGHRVMTASSGPGISLKQEGISFLAGAELPCVIVDIMRAGPGLGNIGPEQADYTQLVKGGGHGNYKNIVLAPDSVQEMCDFTMKAFDLADKYKNPVIILADGVLGQMVEPLQFPSEAVKPEIDTSWAVCGNHETKNNLVTSIFLDFDQLEDFNFKLQEKYDEIKAKEVDYDTYLVEDADIILVSYGISSRISRSAVEKARSMGIKAGLFRPKTLFPFPEKALNDIAQRDCSFISVEMSNGQMMEDIVLAIGCKRPVELVNRMGGNLVDIETVTNKIIEVSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T741</accession>
    <name>F0T741_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleic acid binding OB-fold tRNA/helicase-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646026.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2462"/>
    <dbReference type="EnsemblBacteria" id="ADZ10675">
        <property type="protein sequence ID" value="ADZ10675"/>
        <property type="gene ID" value="Metbo_2462"/>
    </dbReference>
    <dbReference type="GeneID" id="10278934"/>
    <dbReference type="KEGG" id="mel:Metbo_2462"/>
    <dbReference type="eggNOG" id="arCOG03400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157010_0_0_2"/>
    <dbReference type="OMA" id="HITDINR"/>
    <dbReference type="OrthoDB" id="123749at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="47"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="14777" checksum="918E2E8A050F950D" modified="2011-05-03" version="1">MNDETIYKVALTTSILGIVGIIIISGQLYPQEYHITDINRGMLDKQVSIHGVVEEIKKSKTSDTYFLELADETGKIDVVVFHQTVEDYEKYNLKISELIKRRIRIMGTVTEHDGHLEIILTDSKSVKLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T745</accession>
    <name>F0T745_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646030.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2466"/>
    <dbReference type="EnsemblBacteria" id="ADZ10679">
        <property type="protein sequence ID" value="ADZ10679"/>
        <property type="gene ID" value="Metbo_2466"/>
    </dbReference>
    <dbReference type="GeneID" id="10278938"/>
    <dbReference type="KEGG" id="mel:Metbo_2466"/>
    <dbReference type="eggNOG" id="arCOG07599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662398_0_0_2"/>
    <dbReference type="OMA" id="FEYCEGG"/>
    <dbReference type="OrthoDB" id="118723at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026493">
        <property type="entry name" value="Cys-rich_pep"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04165">
        <property type="entry name" value="methano_modCys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9581" checksum="ECBE99C3012AE827" modified="2011-05-03" version="1">MKAENLNQKCPVCGCTDKDISRKRDAGHNDEAFYIPHVPQGTVGVIKCSNCGHCFEYCTDEQCLVEIHKLVLGENKDKQDEKKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T771</accession>
    <name>F0T771_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646056.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2492"/>
    <dbReference type="EnsemblBacteria" id="ADZ10705">
        <property type="protein sequence ID" value="ADZ10705"/>
        <property type="gene ID" value="Metbo_2492"/>
    </dbReference>
    <dbReference type="GeneID" id="10278966"/>
    <dbReference type="KEGG" id="mel:Metbo_2492"/>
    <dbReference type="eggNOG" id="arCOG03208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158570_1_0_2"/>
    <dbReference type="OMA" id="FAIMAVH"/>
    <dbReference type="OrthoDB" id="109266at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018676">
        <property type="entry name" value="DUF2149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09919">
        <property type="entry name" value="DUF2149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="10956" checksum="60887EB80E7DDD80" modified="2011-05-03" version="1">MSKKRRFLDDSDEEDPTSGIINLTDCMLVLAVGFLVFAIMAMHSNPTLLSESSGTVQNTVTVNGQSQSLNNSPENGSNAGNGYEQVGTVYKDPETGKLIMIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T773</accession>
    <name>F0T773_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2494"/>
    <dbReference type="EnsemblBacteria" id="ADZ10707">
        <property type="protein sequence ID" value="ADZ10707"/>
        <property type="gene ID" value="Metbo_2494"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2494"/>
    <dbReference type="eggNOG" id="arCOG04841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096712_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017199">
        <property type="entry name" value="UCP037409_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09930">
        <property type="entry name" value="DUF2162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="215" mass="24025" checksum="FB24FFFCB69CDA6B" modified="2011-05-03" version="1">MNGLGIALKKISKLNFAFITLIYAIVIFVLLFIIGLNNYLSYDFFKHYIAEITGILGLLIFLAGILTIKNWKCNKEITYLNNPAFTVPVIGGTVGVTSVYVLLIGPNFGNNIELILTIILGLLIISFIAFGVSKFLKKLEKPYPIIVTNFMVLVGFFFIMFAAFIPNIATLSNLHMNPLYLRSTDYVAFLVIAVAGIFLCGVFIQNMRMQKETML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T775</accession>
    <name>F0T775_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646060.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2496"/>
    <dbReference type="EnsemblBacteria" id="ADZ10709">
        <property type="protein sequence ID" value="ADZ10709"/>
        <property type="gene ID" value="Metbo_2496"/>
    </dbReference>
    <dbReference type="GeneID" id="10278970"/>
    <dbReference type="KEGG" id="mel:Metbo_2496"/>
    <dbReference type="eggNOG" id="arCOG02526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04746">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_457599_0_0_2"/>
    <dbReference type="OrthoDB" id="43071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Beta_helix" evidence="3">
        <location>
            <begin position="129"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="2">
        <location>
            <begin position="460"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="2">
        <location>
            <begin position="570"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="673" mass="72321" checksum="6C15D1E4957869A1" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A3</accession>
    <name>F0T7A3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643683.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0079"/>
    <dbReference type="EnsemblBacteria" id="ADZ08332">
        <property type="protein sequence ID" value="ADZ08332"/>
        <property type="gene ID" value="Metbo_0079"/>
    </dbReference>
    <dbReference type="GeneID" id="10276502"/>
    <dbReference type="KEGG" id="mel:Metbo_0079"/>
    <dbReference type="eggNOG" id="arCOG04311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_672030_0_0_2"/>
    <dbReference type="OMA" id="ASKWEFD"/>
    <dbReference type="OrthoDB" id="41653at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="194"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="47961" checksum="5FA4B48129D22676" modified="2011-05-03" version="1">MIEDLIERLYEAASMNRWNDHIRPVELTELDKQAHKMVLAYVIAKFEEKDRNADVNWQKLIEGGIFEFLHRTILTDLKPPIFHKMMEEKAKDLNDHVISKLKGDFKGLKPQFEANFKRYLFDTEYSKFERKILKAAHYLATNWEFRIIYHSSPFIYGIEDTKAEIENQIEDHYDLIGVQKLSLEKKSFGFIDLCGQLRFQERWAHSPRVPKTSVLGHMYIVAITSYLFSLENEACPKRLYNNFFAGLFHDLPEVLTKDIISPVKSSVKGLEDLIKEFEENQMKTRLLPLLPVSWRREMNYFTKDEFVNKVRVEDSIKKGIDFKEINRKYNKNEYYPLDGELIKACDKLAAFIEADLSIKHGITSKKLEEGRMDIYKKFKGKKISGIDFGYLFDYFFKRTFTMESNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B5</accession>
    <name>F0T7B5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4 9">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">purD</name>
        <name evidence="12" type="ordered locus">Metbo_0091</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643695.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0091"/>
    <dbReference type="EnsemblBacteria" id="ADZ08344">
        <property type="protein sequence ID" value="ADZ08344"/>
        <property type="gene ID" value="Metbo_0091"/>
    </dbReference>
    <dbReference type="GeneID" id="10276514"/>
    <dbReference type="KEGG" id="mel:Metbo_0091"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="58022at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="105"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ08344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="441" mass="48291" checksum="99634749906EA1C9" modified="2011-05-03" version="1">MNILVVGTGAREHAICNSIKDAELYSIMSNKNPGIARISKYQIGNEKDIEGVKKFAKSNNIDMAIIGPEAPLEMGIVNELIEEGIGCVGPTKEAARIETDKAFMRDLFETHKIDGSIVYKVFDNVEDAGEFIDNFDKDVVIKPIGLTGGKGVKIVGEHLEDGDAAKKYVDEIINNKISGHAQVVIEERLIGEEFTVQAFVDGDKILPMPAAQDHPHAYEGDQGPITGGMGSYSDATGLLPFLDHKSYRKAVKIMEDTVKAVKTEADPYKGILYGQFMLCSDGPKMVEYNARFGDPEAMNVLPLLKTDFVEICEGIVDGNLKKAEFEHKSTVCKYIVPKGYPETAKAGQVIEVDETKINDAGAMVYYAAVNEKDNKIFTSASRALGIVGVGNTISEAEEICEKATSYVKGDVYHRSDVGTEELVQKRVANMQKLRFEAGLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B7</accession>
    <name>F0T7B7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetolactate synthase small subunit</fullName>
            <shortName evidence="6">AHAS</shortName>
            <shortName evidence="6">ALS</shortName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0093</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0093"/>
    <dbReference type="EnsemblBacteria" id="ADZ08346">
        <property type="protein sequence ID" value="ADZ08346"/>
        <property type="gene ID" value="Metbo_0093"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0093"/>
    <dbReference type="eggNOG" id="arCOG04445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_2"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:acetolactate synthase regulator activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="8"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="18340" checksum="ACC3245B9938B7CD" modified="2011-05-03" version="1">MDENKSHVISALVLHKPGVLQRVAGLFTRRGFNIETITVGPSENEGLARMTIISKGDEKVLEQITKQLNKLIDVIKVRDLEPGITVKRELCLIKVHTATEKARSEVIQYTNIFRGRVVDVSPETLTVEITGDPEKIEALIDLVSVFGIKEIARTGPTAITRGTKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E1</accession>
    <name>F0T7E1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-3B C17-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.131</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.131" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643721.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0117"/>
    <dbReference type="EnsemblBacteria" id="ADZ08370">
        <property type="protein sequence ID" value="ADZ08370"/>
        <property type="gene ID" value="Metbo_0117"/>
    </dbReference>
    <dbReference type="GeneID" id="10276542"/>
    <dbReference type="KEGG" id="mel:Metbo_0117"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_0_0_2"/>
    <dbReference type="OMA" id="VWDDRMV"/>
    <dbReference type="OrthoDB" id="38468at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030789">
        <property type="term" value="F:precorrin-3B C17-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007212">
        <property type="entry name" value="Zf-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04071">
        <property type="entry name" value="zf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="zf-like" evidence="6">
        <location>
            <begin position="260"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="353" mass="39011" checksum="FDE65CCC94AFBAB4" modified="2011-05-03" version="1">MISVIGIGPSREDITIKAMNAIKAADVIITYKSYIKNIQDLTEGKEVILKGMGDEIKRAELAITKSKEGKRVALISSGDPGVFGMGNVIFQIIGKFSGVEVEVVPGVSAANFAAGKLGAPLHDFAVISFSDILTPLSEIKRKIKAASEADFVIAIYNPLSKTRTKPFEESVKILLETRNPDTPVGIVKSLETGVNITITTLKDLPDSDINMSTTIIVGNSMTYVEEGYMITPRGYKVSSSIHPLSREFYENYLEGELNNGPNRSCEYYPCHSHPQNCMFCYCPFYPCGEGSTGGKWIKDKGVWSCEDCEWIHSDDTVECIQTKLPEILGEVDDLKNKKKELLKLRRECIFNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E5</accession>
    <name>F0T7E5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643725.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0121"/>
    <dbReference type="EnsemblBacteria" id="ADZ08374">
        <property type="protein sequence ID" value="ADZ08374"/>
        <property type="gene ID" value="Metbo_0121"/>
    </dbReference>
    <dbReference type="GeneID" id="10276546"/>
    <dbReference type="KEGG" id="mel:Metbo_0121"/>
    <dbReference type="eggNOG" id="arCOG04767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_4_2"/>
    <dbReference type="OMA" id="ELYNDHA"/>
    <dbReference type="OrthoDB" id="81003at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15165" checksum="F419157AD3938104" modified="2011-05-03" version="1">MKKAIIETDKGNIELTLFDKEAPNTVANFEKLAKSGFYNGLTFHRVLPNFVIQGGCPKGNGTGGPGYTIKCEINEHKHGTGALSMAHAGKDTGGSQFFITHSPQPHLDGVHTVFGKVIKGMDVVNKIQANDVMNKVTVVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E8</accession>
    <name>F0T7E8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643728.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0124"/>
    <dbReference type="EnsemblBacteria" id="ADZ08377">
        <property type="protein sequence ID" value="ADZ08377"/>
        <property type="gene ID" value="Metbo_0124"/>
    </dbReference>
    <dbReference type="GeneID" id="10276549"/>
    <dbReference type="KEGG" id="mel:Metbo_0124"/>
    <dbReference type="HOGENOM" id="CLU_1425091_0_0_2"/>
    <dbReference type="OMA" id="YCWKCPM"/>
    <dbReference type="OrthoDB" id="108692at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="24375" checksum="2015F4213639CA3C" modified="2011-05-03" version="1">MAGISDNLEIFRKLSKKHYADLVESLEGKYCWKCPMRTNNSETFCREVDSWVRLSIAFEMGIHDHLREMEIPYGCLEVITAKFVEKQISQGNASNKLQKLNLIQMDKSMGFGIDGGDYLLVQQGPKKLRSGDKVLLPNSCPLSNLWYTKNCTEELPLKVFLVTKVFHKQGVKYIQTSEGLEVPFEYVYGFILKIIKEEDPIYTDLHLNQALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F6</accession>
    <name>F0T7F6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase class III protein subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643736.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0132"/>
    <dbReference type="EnsemblBacteria" id="ADZ08385">
        <property type="protein sequence ID" value="ADZ08385"/>
        <property type="gene ID" value="Metbo_0132"/>
    </dbReference>
    <dbReference type="GeneID" id="10276558"/>
    <dbReference type="KEGG" id="mel:Metbo_0132"/>
    <dbReference type="eggNOG" id="arCOG01730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_2_1_2"/>
    <dbReference type="OMA" id="RTEHFAP"/>
    <dbReference type="OrthoDB" id="72542at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016701">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LigB" evidence="5">
        <location>
            <begin position="38"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="29038" checksum="03D8FE7A3BA47E25" modified="2011-05-03" version="1">MVKKKLTTIFVSHGAPTLPFEDVPARTFLSDLGSKYGNFEAVLCISAHWETVSPVLNAVEINETIHDFYGFAPELYQVEYHAEGSPELAKHTQNLITDAGISCDLDFTRGLDHGAWIPMLLMYPDRDVPIFQLSIQQGLDPKQHLALGRALEPLTEERVLIVGSGGAVHPLGYASLHPDAFTDDWAIEFNEWLKIALTTGDTESLVSYRDRSPYSERAHPYPDHFMPLLTAMGAAGEGATGKLIHDSWYWGDLGMGAYEFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J9</accession>
    <name>F0T7J9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644918.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09567">
        <property type="protein sequence ID" value="ADZ09567"/>
        <property type="gene ID" value="Metbo_1326"/>
    </dbReference>
    <dbReference type="GeneID" id="10277777"/>
    <dbReference type="KEGG" id="mel:Metbo_1326"/>
    <dbReference type="HOGENOM" id="CLU_2597752_0_0_2"/>
    <dbReference type="OrthoDB" id="139954at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="79" mass="9193" checksum="BD447AB8341CBDFA" modified="2011-05-03" version="1">MTKINKEQEPSLNTLNRVLNGTLDIRNEYIIAYEGELPRLAVYLIQHDIPFEFSTKGRQIKIPIENVHIESNEIENNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L9</accession>
    <name>F0T7L9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">priS</name>
        <name evidence="13" type="ordered locus">Metbo_1347</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="12">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644938.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1347"/>
    <dbReference type="EnsemblBacteria" id="ADZ09587">
        <property type="protein sequence ID" value="ADZ09587"/>
        <property type="gene ID" value="Metbo_1347"/>
    </dbReference>
    <dbReference type="GeneID" id="10277798"/>
    <dbReference type="KEGG" id="mel:Metbo_1347"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_1_0_2"/>
    <dbReference type="OMA" id="GFDHTGE"/>
    <dbReference type="OrthoDB" id="95578at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="PTHR10536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 10 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 10 11" id="KW-0639">Primosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="325" mass="37566" checksum="DA4B826F4EB3B3BA" modified="2011-05-03" version="1">MVKIEYLTPATPEERKRYYKEEWNIKQVPDFISDTIQDREFGFDHVGRGPNDRYKIFRTPDFLRRFLKVRTPFAAYSSVAFYDKPQRRDGWNGAELVFDVDAKDIPVRTCECEGVCEICLNEAREIVCGLIDVLKGDLGLKDIHVVYSGRGYHLRILDPTVLKADSDVRAQIVKYIVGADVPENEFSLDFERVSYEHFVVPFGYPKVFTDRVKFTILHLNKKAQLDDVNKKLLSDVLKNRNLIANNQWGVFKGKIGPQRYKKVVKGIAALNMSLVDAKVSIDLKRILRLPSSLHSMVSMKCMEVKSIEDFDPFKEAVPKFVYERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M2</accession>
    <name>F0T7M2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1350"/>
    <dbReference type="EnsemblBacteria" id="ADZ09590">
        <property type="protein sequence ID" value="ADZ09590"/>
        <property type="gene ID" value="Metbo_1350"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1350"/>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_248839_0_0_2"/>
    <dbReference type="OMA" id="WTANTEP"/>
    <dbReference type="OrthoDB" id="17702at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010221">
        <property type="entry name" value="VCBS_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="SSF49313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01965">
        <property type="entry name" value="VCBS_repeat"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1475"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="220"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1178"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1179"/>
            <end position="1200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1497" mass="153384" checksum="6AEB25EFD6A7E694" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q3</accession>
    <name>F0T7Q3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho 3-hexuloisomerase</fullName>
            <ecNumber evidence="3">5.3.1.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2522"/>
    <dbReference type="EnsemblBacteria" id="ADZ10735">
        <property type="protein sequence ID" value="ADZ10735"/>
        <property type="gene ID" value="Metbo_2522"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2522"/>
    <dbReference type="eggNOG" id="arCOG00068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094236_1_0_2"/>
    <dbReference type="OMA" id="KTIQPMG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043800">
        <property type="term" value="F:hexulose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="PTHR43443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="39"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="22439" checksum="B2334E9C8736167A" modified="2011-05-03" version="1">MNINKLFGEEMIINDAIEEIIENVMAVSAETDEEHVNDMMDILTSSKNVFLLGQGRSGLVARAFAMRLMHLGISVYVVGETITPAIGEEDCLLAISGSGETSYIISTAMIAKKRGAKIVAVTSYEKSTLGTISDLIMHIKGRTKVDSEKNYIKRQMNGKHLSLSPLGTLFEVSTLIFLDALIAQLMDKMGKTEDDLKKRHTVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S6</accession>
    <name>F0T7S6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein, MJ0208 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643764.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0161"/>
    <dbReference type="EnsemblBacteria" id="ADZ08413">
        <property type="protein sequence ID" value="ADZ08413"/>
        <property type="gene ID" value="Metbo_0161"/>
    </dbReference>
    <dbReference type="GeneID" id="10276587"/>
    <dbReference type="KEGG" id="mel:Metbo_0161"/>
    <dbReference type="eggNOG" id="arCOG01705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="OMA" id="IVWCITG"/>
    <dbReference type="OrthoDB" id="90535at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901285">
        <property type="term" value="P:5,6,7,8-tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
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    </dbReference>
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        <property type="entry name" value="DmrX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02700">
        <property type="entry name" value="flavo_MJ0208"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="24684" checksum="DB7E4C090846B066" modified="2011-05-03" version="1">MRIAWGFTGAGHLLKESVDALVKLSKDHEVTVMTSGAGEEVLKMYGLFETVKNLTGGKYRELVLESDQRESYPMTGRFSLATYELLVVSPATSNTIAKIVNGIADSLITNAVAQAGKGKVKTYIVPVDLESGDVETILPSKLELETCKNCEPCQAAAACPQDAITPGVEINLLKCEGCGTCQTACPFNAVTGGKIITIHMREIDVENTSKLNNIEGINILSHPKELNNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U8</accession>
    <name>F0T7U8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0183"/>
    <dbReference type="EnsemblBacteria" id="ADZ08435">
        <property type="protein sequence ID" value="ADZ08435"/>
        <property type="gene ID" value="Metbo_0183"/>
    </dbReference>
    <dbReference type="GeneID" id="24964513"/>
    <dbReference type="KEGG" id="mel:Metbo_0183"/>
    <dbReference type="eggNOG" id="arCOG06495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887438_0_0_2"/>
    <dbReference type="OMA" id="YYHESHD"/>
    <dbReference type="OrthoDB" id="97166at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="36734" checksum="6E50E6173C7E6797" modified="2011-05-03" version="1">MDNKGYVMGGIAFLLIIPALILANILASAVITDEVHIIPFKSDKLHQLSGDVESNISLITLQILNETSDTVSRTGEPVPNSKFIIKSRIQSKIDESYLEYQRETGINVTCKINSVDNSKDPYKVCVNYTLYANYHEGYIAKNMSQDVQFACSDFTDSQTCKIKDPLPFIKLKGYGSLKTEGDTIFYGASLSNYLKSKGIENSTAYDNASSPLYFKRCPYDPYPFHGHSNSYSTLKNCIDNGYYHLSAAGPCIFCRFEGNSVCNHTGLETFVVPGTSNLSCEYAPCSVDHVIFGASPSEIYLGKLLSYDSNTPQWGMFLDKGHRDKYGLPCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V7</accession>
    <name>F0T7V7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643795.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0192"/>
    <dbReference type="EnsemblBacteria" id="ADZ08444">
        <property type="protein sequence ID" value="ADZ08444"/>
        <property type="gene ID" value="Metbo_0192"/>
    </dbReference>
    <dbReference type="GeneID" id="10276618"/>
    <dbReference type="KEGG" id="mel:Metbo_0192"/>
    <dbReference type="eggNOG" id="arCOG04130">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OMA" id="IGKKLMW"/>
    <dbReference type="OrthoDB" id="112466at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="PTHR40734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="194" mass="22359" checksum="060ABA3C76417AD3" modified="2011-05-03" version="1">MEDNAIILDFLPLGYVKEGKPSFKRKPIAQAIGTEEFTLLELIPKERIQLDIHERVYIGAGKRDKIARVNTRLMFDKLTATSRVELDYVIEEIIKENEAKFVEFFNEAGPISTRLHQLELLPGIGKKHMWDIINATKEAKFTSFEDIKTRVPMLADPVKLIAKRVHMELEAAGDRRGKMKYIIFTRPPKPQKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X7</accession>
    <name>F0T7X7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643815.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0212"/>
    <dbReference type="EnsemblBacteria" id="ADZ08464">
        <property type="protein sequence ID" value="ADZ08464"/>
        <property type="gene ID" value="Metbo_0212"/>
    </dbReference>
    <dbReference type="GeneID" id="10276638"/>
    <dbReference type="KEGG" id="mel:Metbo_0212"/>
    <dbReference type="eggNOG" id="arCOG04881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060249_0_0_2"/>
    <dbReference type="OMA" id="HAFTPWT"/>
    <dbReference type="OrthoDB" id="29545at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00375">
        <property type="entry name" value="TIGR00375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="387" mass="43217" checksum="6501BD5ABFC2DA77" modified="2011-05-03" version="1">MIINADLHIHGCYSMATSKNMTPLVMAPQAKLKGLDVVATGDALHQKYLKIIEDTTEESSDGIFSIKPDLVEDETAESKFILTTEVEDTKRVHHLIIFPSIEAVKETRTKLRGNMDADGRPRIRMNGREIMEVAHETGCIIGPAHAFTPWTSIYKAHDSLVECYGETPDFLELGLSADSDMADKIEELQDLTFLTNSDAHSPWPHRLGREFNEMDVDELTFAGIKDSIANNKITANYGFDPRLGKYHVTACSKCHVKYPVEDAIKMKMKCPCGGRIKKGVDYRIHELSNWEEPHHPPHRPPYIHILPLAEIISITHGKGVTTVFVQNIWKEMINNFENEINALIHAPIQDVVAVDPEVAAVIKSFRNGTLEISSGGGGRYGEIIVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T805</accession>
    <name>F0T805_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644982.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1391"/>
    <dbReference type="EnsemblBacteria" id="ADZ09631">
        <property type="protein sequence ID" value="ADZ09631"/>
        <property type="gene ID" value="Metbo_1391"/>
    </dbReference>
    <dbReference type="GeneID" id="10277842"/>
    <dbReference type="KEGG" id="mel:Metbo_1391"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137753_1_0_2"/>
    <dbReference type="OMA" id="LHWHFIP"/>
    <dbReference type="OrthoDB" id="93754at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17988" checksum="873B1FB7EA1D7122" modified="2011-05-03" version="1">MDCSYCKKLEKYDFGDFIMETTHWIIFLAPQQSNIGTCVVALNRHESDLSGLTREEWLDFGELVREMEETLKKCFDVTLFNWGSLMNASYLKETPDPHVHWHLIPRYDHAVEFEGLVFEDIYFGTMKPRPFRGLPDDVRREIINFIKANHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T838</accession>
    <name>F0T838_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MIP family channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1426</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645015.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1426"/>
    <dbReference type="EnsemblBacteria" id="ADZ09664">
        <property type="protein sequence ID" value="ADZ09664"/>
        <property type="gene ID" value="Metbo_1426"/>
    </dbReference>
    <dbReference type="GeneID" id="10277877"/>
    <dbReference type="KEGG" id="mel:Metbo_1426"/>
    <dbReference type="eggNOG" id="arCOG04431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_2_2"/>
    <dbReference type="OMA" id="IFKALMY"/>
    <dbReference type="OrthoDB" id="45822at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="PTHR45724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="SSF81338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="25529" checksum="EB730A00A2498C24" modified="2011-05-03" version="1">MANLTKRCLAEFIGTFFLVFMGTGAAVIALMISNGAATPNSFNIGIGALGGLGDWLAIGLAFGLAIAAAIYALGNVSGCHINPAVTIGLWSVKKFPGRDVVPYIVSQVLGASLGSFLLAGIIGMGAVTVGGLGATAPFPGIGYYQAMLAEIVGTFLLMLAIMGVAVDRRAPPGFAGIIIGLTVAGVITTVGNISGSSLNPARTFGPYLGDAVLGGNSLWAFLPIYIIGPVIGAVLAALAYNYMTSEEKAIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T898</accession>
    <name>F0T898_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643861.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0258"/>
    <dbReference type="EnsemblBacteria" id="ADZ08510">
        <property type="protein sequence ID" value="ADZ08510"/>
        <property type="gene ID" value="Metbo_0258"/>
    </dbReference>
    <dbReference type="GeneID" id="10276690"/>
    <dbReference type="KEGG" id="mel:Metbo_0258"/>
    <dbReference type="eggNOG" id="arCOG04880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140581_0_0_2"/>
    <dbReference type="OMA" id="WKGHTIL"/>
    <dbReference type="OrthoDB" id="61635at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="25372" checksum="5AF311BE21BEA804" modified="2011-05-03" version="1">MPSYKEHVLAAIIMVFPFFQDVFYIALAVIGASMIDMDHQVNQKNITIVGLLGIILALILYILHLPYLVGILIAVMALLFYLSEHRGFMHSLLGIIFISACVGFFVLGAYTLMTDYGISLKITLIVISLILGIVILNKKLVPIYAFIIILALLFTSNQVFNTYYVFIALLIGAISHLILDLFTKAGVELLSPITSQKFHKLAGFTLLGLYLGAVMVSVVMYGNHFMWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D4</accession>
    <name>F0T8D4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0294</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643897.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0294"/>
    <dbReference type="EnsemblBacteria" id="ADZ08546">
        <property type="protein sequence ID" value="ADZ08546"/>
        <property type="gene ID" value="Metbo_0294"/>
    </dbReference>
    <dbReference type="GeneID" id="10276726"/>
    <dbReference type="KEGG" id="mel:Metbo_0294"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_7_0_2"/>
    <dbReference type="OMA" id="YGICELC"/>
    <dbReference type="OrthoDB" id="83765at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="4">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20171" checksum="A9B757881E450EE6" modified="2011-05-03" version="1">MEMDTKVTVHDAMTPSVITADPKTTVVDAAVLMSRFKIGSLVIKGETGPLGIITESDIIAKVVAKNLTANEINIGQIMTKDLIFIDPGSELNQAARIMAKNSIRRLPVIDNGILVGILTSTDVLMVSPELTELLVENARMENQKEFTDSDSSVPGTCEVCGNYVDYLDVFDGKFVCEECKEDLDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E6</accession>
    <name>F0T8E6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645048.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1460"/>
    <dbReference type="EnsemblBacteria" id="ADZ09697">
        <property type="protein sequence ID" value="ADZ09697"/>
        <property type="gene ID" value="Metbo_1460"/>
    </dbReference>
    <dbReference type="GeneID" id="10277911"/>
    <dbReference type="KEGG" id="mel:Metbo_1460"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_745169_0_0_2"/>
    <dbReference type="OMA" id="WPISTLP"/>
    <dbReference type="OrthoDB" id="88040at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="371" mass="39703" checksum="F7242577388CED26" modified="2011-05-03" version="1">MENENFKAMVSNIARKGFKNKQTLGFAVVAPLIVLIVLGYMVTMAGYTEPVKIGVINNDKGIGNFSAATSIVNEIKNQENVTVLYINEADVKNDLDDKTITAAVTFPENFTTSLAKNNADLNIELEGTDQTANMLVSKAVSTSVSTVAAKTTNQTSPLKINVDNLYGNGLDFTDLFMYRFMTIVTLVLSVLLGLFSIMGDKETEVFKNRAATPIKTVFAYITGLSVFALILALITLAFGIYALGLTIVGNTGAAALLMFLIAMVGLSLGVLIASITRTRTQGMGLFGLLLILQIVFSGTFVPVSKFNSLTQLISYSLPLTYSLDSMKSIFIKGFTLGEVGTDITALIIIFAVALILSMIGLKTVQGTGNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F0</accession>
    <name>F0T8F0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1464"/>
    <dbReference type="EnsemblBacteria" id="ADZ09701">
        <property type="protein sequence ID" value="ADZ09701"/>
        <property type="gene ID" value="Metbo_1464"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1464"/>
    <dbReference type="HOGENOM" id="CLU_3245336_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="42" mass="4668" checksum="47673A17D0537A09" modified="2011-05-03" version="1">MSKNESEERLQILEMVEVGQISASEAMTLLDALEILRTKGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F5</accession>
    <name>F0T8F5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
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    <dbReference type="KEGG" id="mel:Metbo_1470"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="459" mass="50895" checksum="45B2AF48FABCC104" modified="2011-05-03" version="1">MKIGEIVKDSLKYPFSDWKKFLIFGILVMFNSLNAIFQSLGMDRLLISMLGIIGLLFGVLVFGYEIRILKLSLNGVAKLPPFNDWFDIIINGIFVIVVAIAYSIPLIMILMFGGLSLGLTIGSIGTNGATIFLVILIAIIFLYLIIISPIFLMSLANMAFYNDDLGAAFKFRDIFYKISNIGWGNLIIWYITIAIICMGILFLGAVIEVFFNLIGLKVIGVVLIPLIVLPYLSIYIFRSVALFYLSEDQGFLECEKCGGYYELQPGESLEDFEKCQCGGELKYNKQPDKRDNRSFIENLKSLNKKQGLIIVGILALIIGMPFIFAQHMPQHIITTNSTLIGTYNVSNLNNPNSVGTFVVIPPGTTNIKVEYNLSWTPAKTGANGFDITGYNTNITGWDSLPDNKNVIYNKGISLFENGQNKTGTLNLDGNTIRSLVISGNGVNGTVKIYAIKTKFTLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1493"/>
    <dbReference type="EnsemblBacteria" id="ADZ09725">
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        <property type="gene ID" value="Metbo_1493"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OMA" id="IWGMVIG"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
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    </dbReference>
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        <location>
            <begin position="17"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    <sequence length="471" mass="51076" checksum="8309BF7E29F6C340" modified="2011-05-03" version="1">MNEKEVVKKQLNVGWFPLIIVTLAAFIISIDISFLNVAITNLVNDLHTTVVTIQSIIVVYSLVLASLTLLSGELQKVLGRRKTFLLGAFIFGVGNFIAALSINSTMLLIGWSVLEGAGGALMWVSMYSIVIGSYTGNRRATALGLSGSIGSLGLVVGPFIGGFLTTYLSWRYAFGLEFIIILLILVFSRHIPSFPATMHWSDINIVGGINSALGIFLLVYGLILLNDPSTLYIGPYVILAGIILLAVFFYDQRSRIKNDKHPLVDIRIFKIRSFALGNIFLFLYGSVVSGIRFVIPVFVQSVLGYSALLTGYLFAAMALPMFFVTFTTGRISGRFHPRHIISIGFIVCLIGAGYLIYAFSSNPSFIEMIIGMAVIGLGIGIIAPHGSTYAFSEIKHDKQPDASAIRSTNSNLNSSVGTAIFGLILLMGAANALNVEQLSTGMLDSIYAMGILLFVGLIMTQFIKMNKITES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K5</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    <reference evidence="3" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Metbo_1526"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="38" mass="4306" checksum="AE517E8B08A7EDCE" modified="2011-05-03" version="1">MIQTVTTTTTTIRMPLIVIVSIILILLIIIILIKRKKS</sequence>
</entry>
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    <accession>F0T8L8</accession>
    <name>F0T8L8_METLA</name>
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        <submittedName>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="4">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_0308"/>
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    <dbReference type="KEGG" id="mel:Metbo_0308"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OrthoDB" id="67226at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
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        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
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        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
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        <property type="entry name" value="FAD-bd_FR_type"/>
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        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
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        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="29055" checksum="4AEC0BC0B7D490BB" modified="2011-05-03" version="1">MHVPKIVEIKRVVKESPTVKTFIFDWEVHEEVPGNFMMVWNFKDEKPMSISLIDPVNNEIGISIRGVGEFTNQVHDLKEGDLLGLRGPYGRGFHMAGPKILAVGGGVGMAPIVAFTEEASRRGFSVDVVSAAGTANELLFVERIKKTGSKLLTCTDDGSHGFCGFGTELADIALSETNYDMVVACGPEVMMKKLYSTVLELDIPAQFSLERYMKCGMGLCGQCCVDDLGWRVCVEGPVFWSDELKMISEFGKYKRDASGVKHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N6</accession>
    <name>F0T8N6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643929.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0326"/>
    <dbReference type="EnsemblBacteria" id="ADZ08578">
        <property type="protein sequence ID" value="ADZ08578"/>
        <property type="gene ID" value="Metbo_0326"/>
    </dbReference>
    <dbReference type="GeneID" id="10276763"/>
    <dbReference type="KEGG" id="mel:Metbo_0326"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="IVMANEM"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="132"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="203"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="267"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="52563" checksum="7974DC7F56C2FACB" modified="2011-05-03" version="1">MNKKNLLIVEDEGVTALEIQNKVEEWGYSVVGVLSSGEEAITVALDKRPDLILMDVVLKEKVNGIEAANIIKNSYDVPIIYLTAYDDEKTIEEAKITFPQAYLLKPFNDQELKLTIEIALYKHQMEARLKRSEETYRTLAENAEDIIFIISLEDRVDYVNKFAANILGFEPEDIVGKLRQNLFPMDISEKQRKSIDKAIETQSPVRSEIKMALPKGEMWLDTKIIPLKDKNDKIFAVMGISRDVTTQKNTEAALRSSLNEKVVLLKEIHHRVKNNMQIISSLINLQSDYATDESTIKMFEDSKHRIRTMALIHEKLYMSEDISLINFSKYIKSLTTKLLEFYSLKSRLITLKVISDDITMDIDSAIPCGLLINELLSNSIKYAFPDGREGNIVIKMHINDGYYVLSVEDDGVGFPEEIDFMNPNTLGLQIVQTLAQQLDGNIELETNGFTRFKISFKVENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P2</accession>
    <name>F0T8P2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643935.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0332"/>
    <dbReference type="EnsemblBacteria" id="ADZ08584">
        <property type="protein sequence ID" value="ADZ08584"/>
        <property type="gene ID" value="Metbo_0332"/>
    </dbReference>
    <dbReference type="GeneID" id="10276769"/>
    <dbReference type="KEGG" id="mel:Metbo_0332"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079270_3_0_2"/>
    <dbReference type="OMA" id="AVFRMFG"/>
    <dbReference type="OrthoDB" id="96137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="240" mass="26802" checksum="CFA7C44C8F15C12E" modified="2011-05-03" version="1">MDIGYVISDSMKYPSTNWKNVIILGLLFMFSFLIVPLFLLYGYVFRVIKASLAGIEDLPDFEEWGEMLVDGIKLFLVHIIYMLPAMILVIYSIFAFAMALHSFSNLNPTTTLNPTLIYSLIGGSAAVGLVLAAIYSLIIYPIMTVGIGNMAFHNGDLGSAFKIGDILTIISEIGWVDLIIWYVAMIIICITIFVAGTIIAMIPLLGWLLLTLIVYPYLYLFFGRALAWLYASAFTEEYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R3</accession>
    <name>F0T8R3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyl-coenzyme M reductase subunit alpha</fullName>
            <ecNumber evidence="10">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metbo_0353</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="10">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643956.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0353"/>
    <dbReference type="EnsemblBacteria" id="ADZ08605">
        <property type="protein sequence ID" value="ADZ08605"/>
        <property type="gene ID" value="Metbo_0353"/>
    </dbReference>
    <dbReference type="GeneID" id="10276790"/>
    <dbReference type="KEGG" id="mel:Metbo_0353"/>
    <dbReference type="eggNOG" id="arCOG04857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="OMA" id="AMLYDQI"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_alpha_N" evidence="14">
        <location>
            <begin position="3"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_alpha" evidence="13">
        <location>
            <begin position="316"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Pros-methylhistidine" evidence="12">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="12">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ08605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="550" mass="60359" checksum="4D976CC6EAAC6EBA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R9</accession>
    <name>F0T8R9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetrahydromethanopterin S-methyltransferase subunit F</fullName>
            <ecNumber evidence="14">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mtrF</name>
        <name evidence="16" type="ordered locus">Metbo_0359</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cell membrane</location>
            <topology evidence="3 14">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the MtrF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="14"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643962.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0359"/>
    <dbReference type="EnsemblBacteria" id="ADZ08611">
        <property type="protein sequence ID" value="ADZ08611"/>
        <property type="gene ID" value="Metbo_0359"/>
    </dbReference>
    <dbReference type="GeneID" id="10276796"/>
    <dbReference type="KEGG" id="mel:Metbo_0359"/>
    <dbReference type="eggNOG" id="arCOG03381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204306_0_0_2"/>
    <dbReference type="OMA" id="ATRIYGM"/>
    <dbReference type="OrthoDB" id="122652at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01099">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011307">
        <property type="entry name" value="MeTrfase_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013347">
        <property type="entry name" value="MeTrfase_F_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09472">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 14" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="8 14 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 16" id="KW-0808">Transferase</keyword>
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    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="61"/>
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        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ08611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="67" mass="7357" checksum="23DA2CE9C07F3292" modified="2011-05-03" version="1">MILSNKPNVRGIKSTVKDIKYRTGLIGRDQRLFAGLITTRVYGMAVGFIFALLLVGLPVIYKTMVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S5</accession>
    <name>F0T8S5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0365"/>
    <dbReference type="EnsemblBacteria" id="ADZ08617">
        <property type="protein sequence ID" value="ADZ08617"/>
        <property type="gene ID" value="Metbo_0365"/>
    </dbReference>
    <dbReference type="GeneID" id="10276802"/>
    <dbReference type="KEGG" id="mel:Metbo_0365"/>
    <dbReference type="eggNOG" id="arCOG02155">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163105_0_0_2"/>
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    <dbReference type="OrthoDB" id="111827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="16"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="10030" checksum="12D4E11AE78F717E" modified="2011-05-03" version="1">MVVEERKLIKGEEKVWSEIKGYQVATNNARILGELEELVINGKTGKITDVVIKVEKGRNVHIKGAKKKGDNLLVPFGKVEKVGEFIIISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V0</accession>
    <name>F0T8V0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645129.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1550"/>
    <dbReference type="EnsemblBacteria" id="ADZ09778">
        <property type="protein sequence ID" value="ADZ09778"/>
        <property type="gene ID" value="Metbo_1550"/>
    </dbReference>
    <dbReference type="GeneID" id="25394734"/>
    <dbReference type="KEGG" id="mel:Metbo_1550"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016295_0_0_2"/>
    <dbReference type="OMA" id="PREYETK"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="353"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="487"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="684" mass="78154" checksum="E48312AB1481DC8E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T917</accession>
    <name>F0T917_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylglycerophosphatase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0387"/>
    <dbReference type="EnsemblBacteria" id="ADZ08639">
        <property type="protein sequence ID" value="ADZ08639"/>
        <property type="gene ID" value="Metbo_0387"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0387"/>
    <dbReference type="eggNOG" id="arCOG01869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064440_0_0_2"/>
    <dbReference type="OMA" id="ICTDDIT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PgpA" evidence="1">
        <location>
            <begin position="226"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="356" mass="40177" checksum="7CF4171C07B194A2" modified="2011-05-03" version="1">MNICIDKVKIKKDEQTMLIYREDGFEVMGSTTTGNGHGHVNSIVYHGLDNQKHPVPYNDEEIEEHERVMERNEYLTTVLDFKGVKEPAASILLPLKNFEFEICSDTDYTILVSAVKDSALFLNILVFVDHDLNPKTMVKMYKSIIESKMAALWDLGVINHFSFDPVDTDDDESILIAAKGSSDEFNYKLDHDIISRVQRSVRETVKTALNKEGFPKGVIEFMRDVGVEVDDLVDAGMQLIVGVDETTELREKLKAQIYHSLEDLNVVSFIIAGIRLEEDYEKHRVRGVDVDDDPAYLYSDEVFGMSVANQIAGTKAIFNFKRYDEEKPGIISELGPVLDDIFAGVVAGCMSKIFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T921</accession>
    <name>F0T921_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative nickel insertion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LarC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643994.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0391"/>
    <dbReference type="EnsemblBacteria" id="ADZ08643">
        <property type="protein sequence ID" value="ADZ08643"/>
        <property type="gene ID" value="Metbo_0391"/>
    </dbReference>
    <dbReference type="GeneID" id="10276828"/>
    <dbReference type="KEGG" id="mel:Metbo_0391"/>
    <dbReference type="eggNOG" id="arCOG02701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028523_2_1_2"/>
    <dbReference type="OMA" id="TCLGLDW"/>
    <dbReference type="OrthoDB" id="69338at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01074">
        <property type="entry name" value="LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="PTHR36566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="DUF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00299">
        <property type="entry name" value="TIGR00299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 2" id="KW-0533">Nickel</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="44310" checksum="9D3F38B650A84E36" modified="2011-05-03" version="1">MTLIVDPQNSGISGNMVVGALVDFGVDNEVLKEVMEHYGSFFGKVEVTINKTIKAGISSSFVQVKARDSQAVTYKELIETLDGIEHPEITEEIREFAKKVYKTMAEAESHVHGTTMDKVHFHEVGAADAVADVIGASYLFHKLELKKAKVYGMPVALGGGRIDSMHGKLAVPAPATLEILKTVPTFGGPVDKELTTPTGAALYMNMVDEFIKFYPLLSNKTVGYGAGKMELEYPNVLRMIKGVGEMEHENVSILETNVDDVSGEVLGHIFEGLMKSGARDVSIIPTITKKNRPGHLLRVIAKPKDTEQISEELIRETGTLGVRVLPYAHRNIAQRKIVPMNVIIDGKPYKIDIKVGMIGEDVISVKPEYEDVKHVAYLTGRQLKYVTDIAVQQFRNSEFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T937</accession>
    <name>F0T937_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644010.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0407"/>
    <dbReference type="EnsemblBacteria" id="ADZ08659">
        <property type="protein sequence ID" value="ADZ08659"/>
        <property type="gene ID" value="Metbo_0407"/>
    </dbReference>
    <dbReference type="GeneID" id="10276844"/>
    <dbReference type="KEGG" id="mel:Metbo_0407"/>
    <dbReference type="eggNOG" id="arCOG02882">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056369_0_0_2"/>
    <dbReference type="OMA" id="CHLHPEI"/>
    <dbReference type="OrthoDB" id="42075at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017667">
        <property type="entry name" value="Methan_mark_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03266">
        <property type="entry name" value="methan_mark_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00702">
        <property type="entry name" value="TIGR00702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="69"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="399" mass="44679" checksum="DE800C1F88C3E330" modified="2011-05-03" version="1">MLNEVPVKYFGCTHRAVAPEKTIENVEPKLRAAGVTRVAEITHLDRIGIPVYSAIRPGAAEGAVSIYAGKGATKSQAKASAMMESFERFSAEITDLDRKNFVRGNFEESDLHNYLDPDKLILPKLGFNSKTEGLEWVKAVDITNDKTVFVPANAVYHPYDSENISKLFQSNTNGLASGNLIEEAIFHGMMEVVERDAWSIFEARHKPKPEINLETIENPLINNILHLFKKAGIHVKLVNLTADVEITTIAAVSDDTVLKDPALLTLGVGTHLDPEVAVIRALTEVAQSRATQIHGTREDTVRAVFMRKAGYERMKRINKHWFGESQSEVDLKEIRNYSGKTFKEDIETSQKLLGKQGFKDILYVDLTRQEIQIPVVRVLIPEMELFSVDVNRAGKRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T938</accession>
    <name>F0T938_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644011.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0408"/>
    <dbReference type="EnsemblBacteria" id="ADZ08660">
        <property type="protein sequence ID" value="ADZ08660"/>
        <property type="gene ID" value="Metbo_0408"/>
    </dbReference>
    <dbReference type="GeneID" id="10276845"/>
    <dbReference type="KEGG" id="mel:Metbo_0408"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="OMA" id="SEFRAWY"/>
    <dbReference type="OrthoDB" id="118207at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="23407" checksum="28C0127B71842152" modified="2011-05-03" version="1">MVSFTAREVRDILISMFVIAAIFAYLFSKGLLANGTEIFLNNFILLIPALLVSVGLGFVLHELAHKFVAVRYGFYAEFRMWVEGLMFALFTAFIFSVVFVAPGAVYIHGTNISKEQNGKISIAGPLVNIALALFFLSLFPLVSHNPTDIVGYELHVIVTYGFIINSLLAVFNLIPFGPLDGGKVLSWNPVIWILIFIMAIMLAIKAYFGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T946</accession>
    <name>F0T946_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">T-RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0416</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644019.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0416"/>
    <dbReference type="EnsemblBacteria" id="ADZ08668">
        <property type="protein sequence ID" value="ADZ08668"/>
        <property type="gene ID" value="Metbo_0416"/>
    </dbReference>
    <dbReference type="GeneID" id="10276853"/>
    <dbReference type="KEGG" id="mel:Metbo_0416"/>
    <dbReference type="eggNOG" id="arCOG04359">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110988_0_0_2"/>
    <dbReference type="OMA" id="WDTSKDY"/>
    <dbReference type="OrthoDB" id="88074at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041169">
        <property type="entry name" value="Alpha_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18489">
        <property type="entry name" value="Alpha_Helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="126"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="237" mass="26190" checksum="BC876D34504D8767" modified="2011-05-03" version="1">MWDSSKDYRLRVLEKSVELFIKTAEGANLKGIWNKKKCLTSARSMLPEIQIMYFSYMEPAALAESPQVEDMKLKVKDIIDALGGDQWHVQFLNLVNKDEKEKLVESIAKMKFALNTISTVDERLKLGPINDPVIGIDIKTGEIVSIQKKDNLMVCNVNLGTRAITVVTNDLTVKEGNKVGVAVLPPSVFQGVTSEGMFLGAGEGILKNVEGELGTMPHGIPLDALNETRNLVETFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T952</accession>
    <name>F0T952_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0422</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644025.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0422"/>
    <dbReference type="EnsemblBacteria" id="ADZ08674">
        <property type="protein sequence ID" value="ADZ08674"/>
        <property type="gene ID" value="Metbo_0422"/>
    </dbReference>
    <dbReference type="GeneID" id="10276859"/>
    <dbReference type="KEGG" id="mel:Metbo_0422"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_2"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="OrthoDB" id="24697at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="453"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="525" mass="57697" checksum="C629DC0B933FE69C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T953</accession>
    <name>F0T953_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein Metbo_0423</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644026.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0423"/>
    <dbReference type="EnsemblBacteria" id="ADZ08675">
        <property type="protein sequence ID" value="ADZ08675"/>
        <property type="gene ID" value="Metbo_0423"/>
    </dbReference>
    <dbReference type="GeneID" id="10276860"/>
    <dbReference type="KEGG" id="mel:Metbo_0423"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_1_1_2"/>
    <dbReference type="OMA" id="VDFEISM"/>
    <dbReference type="OrthoDB" id="121009at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="12356" checksum="3B35FA01C090EB7B" modified="2011-05-03" version="1">MVSVDEFIIVSSNYVPGYEILETKGFVYGLTVRSRGVGGQLGAGIRSMFGGEIKEYVQMMQESRNEALERCIDHAREMGANAIISARFDSDAISDIMQEILAYGTAVVAQPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T971</accession>
    <name>F0T971_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644044.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08693">
        <property type="protein sequence ID" value="ADZ08693"/>
        <property type="gene ID" value="Metbo_0441"/>
    </dbReference>
    <dbReference type="GeneID" id="10276878"/>
    <dbReference type="KEGG" id="mel:Metbo_0441"/>
    <dbReference type="eggNOG" id="arCOG10458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870768_0_0_2"/>
    <dbReference type="OrthoDB" id="130945at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="16119" checksum="952ACEE1BF7A2878" modified="2011-05-03" version="1">MIDYYYKTIGELVEINVEANSIAESRRLISEINERENILMKLREEVRRDIRSLESNFLKEKARLRTKYSMKQNSGLASIIMGSPKKKLIKELKSLESKSNRDIEELKHIRCVIDDLLIQFNDLREPLNSSMRARFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T993</accession>
    <name>F0T993_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TM2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645195.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09844">
        <property type="protein sequence ID" value="ADZ09844"/>
        <property type="gene ID" value="Metbo_1617"/>
    </dbReference>
    <dbReference type="GeneID" id="10278074"/>
    <dbReference type="KEGG" id="mel:Metbo_1617"/>
    <dbReference type="eggNOG" id="arCOG03293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191146_1_0_2"/>
    <dbReference type="OMA" id="VYAGNIM"/>
    <dbReference type="OrthoDB" id="131056at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="7822" checksum="8BAD0A358B43DF8E" modified="2011-05-03" version="1" precursor="true">MANPILALIISLFLPGIGTVYAGNIMKGIIIFIVALILGALATVFLLGIIAWILYVIVWLYGMYDAYTTASAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T996</accession>
    <name>F0T996_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645198.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1620"/>
    <dbReference type="EnsemblBacteria" id="ADZ09847">
        <property type="protein sequence ID" value="ADZ09847"/>
        <property type="gene ID" value="Metbo_1620"/>
    </dbReference>
    <dbReference type="GeneID" id="10278077"/>
    <dbReference type="KEGG" id="mel:Metbo_1620"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958500_0_0_2"/>
    <dbReference type="OMA" id="GFNIMYI"/>
    <dbReference type="OrthoDB" id="112827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="282" mass="30638" checksum="57EF2CCADB523652" modified="2011-05-03" version="1">MVATVKNLKEKRWGYIAVLAGFLVGFTSAYFCILWHLVIFGFNIMYIISPLIAGVVETLIARQKYGKSTGAISALITFILINIYGWFLPASVIDPTKEPATLSLITIIAIILTLQAAFPTLVNYILMVTAVGFVKKIIGFLLYIPRRLMGKGVKPEESIQIDTATPGVDEKFLDDLPIPILSIAADISKIEKYMGMVSGEAVATERESEGTIEKLTSIIEPVELGDYDMASAREMAISRMLDEAKAIGANSVIEVLVEYVSMGGLQGSSLIITATGTAVKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B4</accession>
    <name>F0T9B4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1639</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645216.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1639"/>
    <dbReference type="EnsemblBacteria" id="ADZ09865">
        <property type="protein sequence ID" value="ADZ09865"/>
        <property type="gene ID" value="Metbo_1639"/>
    </dbReference>
    <dbReference type="GeneID" id="10278096"/>
    <dbReference type="KEGG" id="mel:Metbo_1639"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="OMA" id="MFQVTDP"/>
    <dbReference type="OrthoDB" id="49614at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="PTHR10264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="3">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28926" checksum="4EADB9ABE5B4C7F2" modified="2011-05-03" version="1">MDLITLIIILIVILVILGLSIRIVNQYERGVVFRFGKVIGVKEPGLRLLIPFVDRMVKPSLQIITMPIQSQKIITEDNVSIDVAAVAYFKIIDPYKAVVEIENYTAAVNQISQTTVRSVVGQFNLDEILSVTPKINLKIKEIIDKHSEPWGINVTTVEIKDITLPENMKRVIGLQAEAEREKRAKIIAAEGEYLSASKLGDAADIISEHPIALQLRIMQVLNQIAVEKNSTIIFPAPLMNSITDISNFLKTENMEKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D8</accession>
    <name>F0T9D8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1663"/>
    <dbReference type="EnsemblBacteria" id="ADZ09889">
        <property type="protein sequence ID" value="ADZ09889"/>
        <property type="gene ID" value="Metbo_1663"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1663"/>
    <dbReference type="eggNOG" id="arCOG01153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063034_1_0_2"/>
    <dbReference type="OMA" id="GEHNRNY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="5"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="265" mass="30378" checksum="7725710797C62897" modified="2011-05-03" version="1">MMDKKLIHISDLHFGEYKFDEKLKNNLKFQIEYENPDLIVVAGDITALGYLEDYNLAREFLYELNNIAETYVVPGNHDSCNVGLVHFRKLIGERRFVATDKNEDIVLIGLDSSEPDIHDGKIGFDQIEWLNDVLNKIPRDRFTAVTFHHHLLPIPQTGRERNILLDSGDVLKLLVSHGVNLVLNGHKHVSNAWKIDDMVILNSGTATTNRLYGDNSPSYNKVLVDDETITAYKIKTETGEEKLLAHYTYEQQIHNRSKIPTIFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I2</accession>
    <name>F0T9I2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0478</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644081.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0478"/>
    <dbReference type="EnsemblBacteria" id="ADZ08730">
        <property type="protein sequence ID" value="ADZ08730"/>
        <property type="gene ID" value="Metbo_0478"/>
    </dbReference>
    <dbReference type="GeneID" id="10276918"/>
    <dbReference type="KEGG" id="mel:Metbo_0478"/>
    <dbReference type="eggNOG" id="arCOG00167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_2"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="37194at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="67"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="291" mass="31123" checksum="EE600D85994C36F8" modified="2011-05-03" version="1">MTEKTEEKFVKWVLFATGLSSIAIILLIILFIFNEGIPAITNIGFFNFVFGMTWAPSNGQYGVFPMVIGTLGITALALLLAVPLSICCAVFLSEIAPPTMRKILKPTIQGLAGIPSVIYGFFGLVVLVPFMRVHYGGTGFSLLAASLILTIMILPTITSISEDALRSIPMEYKEASFALGATQWQTIKNVLLPAAVPGVITAIILGMGRAIGETMAVIMVAGNVVQIPGSIFDPVRALTSNIALEMGYATGLHYNALFATGIILIGVIIILLLIANYFNYKKRVTIGGGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N6</accession>
    <name>F0T9N6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1690</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1690"/>
    <dbReference type="EnsemblBacteria" id="ADZ09915">
        <property type="protein sequence ID" value="ADZ09915"/>
        <property type="gene ID" value="Metbo_1690"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1690"/>
    <dbReference type="eggNOG" id="arCOG03209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088835_0_0_2"/>
    <dbReference type="OMA" id="NRWYEEY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="199" mass="21532" checksum="0E7F67702338158D" modified="2011-05-03" version="1">MPVLVILLAVLVYVVIEAGGIISEYSMRKKTEFIEVENFINNISDPGTPENIENVVNASVLPKEQKEILIKLAGSTGIGDKSREVMARKMIEEEELKAIKRTEKTDIIAKIGPALGLIATLIPMGPGLAALGAGDINTLSQNLIIAFDAAILGLTSAAIAYTISKVRKRWYEDQISTLEALAESILEVLENAKETKKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q4</accession>
    <name>F0T9Q4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645284.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1709"/>
    <dbReference type="EnsemblBacteria" id="ADZ09933">
        <property type="protein sequence ID" value="ADZ09933"/>
        <property type="gene ID" value="Metbo_1709"/>
    </dbReference>
    <dbReference type="GeneID" id="10278166"/>
    <dbReference type="KEGG" id="mel:Metbo_1709"/>
    <dbReference type="eggNOG" id="arCOG03571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129665_1_0_2"/>
    <dbReference type="OMA" id="APCGMNC"/>
    <dbReference type="OrthoDB" id="92010at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024227">
        <property type="entry name" value="DUF3795"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12675">
        <property type="entry name" value="DUF3795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="16032" checksum="1B2057DAA1D859BB" modified="2011-05-03" version="1">MNTREYSLIAPCGMNCALCMAFLRDRNKCPGCRGPDDNKSITKVNCKIKKCEIFKTGDAEFCFECEDFPCNRLVHLDKRYKKKYHMSMITNLKSIKSNGLENFLVDEKEKWTCSECGGTICVHKGYCYSCRRVYFEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R8</accession>
    <name>F0T9R8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_1723</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645298.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09947">
        <property type="protein sequence ID" value="ADZ09947"/>
        <property type="gene ID" value="Metbo_1723"/>
    </dbReference>
    <dbReference type="GeneID" id="10278180"/>
    <dbReference type="KEGG" id="mel:Metbo_1723"/>
    <dbReference type="eggNOG" id="arCOG04887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090238_3_2_2"/>
    <dbReference type="OMA" id="PWGWAVM"/>
    <dbReference type="OrthoDB" id="87417at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="210" mass="23785" checksum="FB6BEC83A68503F2" modified="2011-05-03" version="1">MTDSTLNLPTNRIETLADGIFAIAMTLLVLGISVPNLPSSTDPQVFNAYIFSLIPEILVYILSFILLAVFWLNHHVFFVIKKSDITLLWINIFFLMSIAIVPFTTSLIGKYGQFHLSVLIFDLNMLIIGILFYSIFAYAKRHDFLVGSAQKKSASIQRSNLILPILAGVAIIISFFNSKWSVFIFFLLPITFTLYNFARHLFVRNKDHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U2</accession>
    <name>F0T9U2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MtN3 and saliva related transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645322.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1748"/>
    <dbReference type="TCDB" id="2.A.123.1.25">
        <property type="family name" value="the sweet, pq-loop, saliva, mtn3 (sweet) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09971">
        <property type="protein sequence ID" value="ADZ09971"/>
        <property type="gene ID" value="Metbo_1748"/>
    </dbReference>
    <dbReference type="GeneID" id="10278205"/>
    <dbReference type="KEGG" id="mel:Metbo_1748"/>
    <dbReference type="HOGENOM" id="CLU_2447696_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_sugar_transpr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10361" checksum="5D9793B60709B7F0" modified="2011-05-03" version="1">MSINAIEVIGSLACLFTFLMYLSTIDQMRTVLKTENSEEVSEILYWVMFFNCFFWSIYGLYLSNNYILIPNFVGCVLSLTTAAVVWKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V7</accession>
    <name>F0T9V7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2b</name>
        <name evidence="11" type="ordered locus">Metbo_0528</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644131.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0528"/>
    <dbReference type="EnsemblBacteria" id="ADZ08780">
        <property type="protein sequence ID" value="ADZ08780"/>
        <property type="gene ID" value="Metbo_0528"/>
    </dbReference>
    <dbReference type="GeneID" id="10276973"/>
    <dbReference type="KEGG" id="mel:Metbo_0528"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="OMA" id="FIRYYVV"/>
    <dbReference type="OrthoDB" id="96453at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="PTHR23001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="SSF100966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="SSF75689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eIF2B_5" evidence="10">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="15601" checksum="3F2D97DB3CC191FC" modified="2011-05-03" version="1">MADYNELLDRALDQLPQGVEETKRFNVPNAYSMIQGNRTMIQNFTEVTEALNRDPQHVLKFLLRELGTAGNLEGTRAILQGKFTHYLINEKLDDYVKRFIMCHECNRPDTKIIREDRIFILKCEACGAKAPLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X6</accession>
    <name>F0T9X6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pfdA</name>
        <name evidence="7" type="ordered locus">Metbo_0547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644150.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0547"/>
    <dbReference type="EnsemblBacteria" id="ADZ08799">
        <property type="protein sequence ID" value="ADZ08799"/>
        <property type="gene ID" value="Metbo_0547"/>
    </dbReference>
    <dbReference type="GeneID" id="10276992"/>
    <dbReference type="KEGG" id="mel:Metbo_0547"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091867_1_1_2"/>
    <dbReference type="OMA" id="ELQNQFM"/>
    <dbReference type="OrthoDB" id="105878at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PTHR12674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00293">
        <property type="entry name" value="TIGR00293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="15719" checksum="96C90B58A0A912CA" modified="2011-05-03" version="1">MEDRQRLEALMNELNTYQGQAEVIQQQVENLNASIAELTVALDTLEVVKDEDKEETLVPIGAGSFLITELKNTDEVIIGLGAGVAVKKKIADAKETIGEQKTELEELRDKMTSDLQKISDYIRQRSPEAEALMQKVEGEGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y5</accession>
    <name>F0T9Y5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="9">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rtcB</name>
        <name evidence="10" type="ordered locus">Metbo_0556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8 9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644159.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0556"/>
    <dbReference type="EnsemblBacteria" id="ADZ08808">
        <property type="protein sequence ID" value="ADZ08808"/>
        <property type="gene ID" value="Metbo_0556"/>
    </dbReference>
    <dbReference type="GeneID" id="10277001"/>
    <dbReference type="KEGG" id="mel:Metbo_0556"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="OMA" id="QTRGVEC"/>
    <dbReference type="OrthoDB" id="22545at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="PTHR11118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="SSF103365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="6">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="203"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="329"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="378"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="404"/>
            <end position="407"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="481" mass="52625" checksum="DEE77942163A35EA" modified="2011-05-03" version="1">MVMEELKKVRDCVWEIPSSYKKGMRVPGRIYQDDEGIEDLEKGAADQVANVATLPGIQKFSIGLPDIHFGYGFSIGGVAAFSSRTGVISPGGVGFDINCGVRMVRTNLTEDDVKPRIKELIDTLFKNVPSGVGSKGKVRLKEGEIDDVLNNGAEWAVENGYGWESDLKFLEENGKMESADSEAVSAKAKKRGIPQLGSLGSGNHFLEIQKMEEIYDEKAAKAFGIEEGQITIMIHSGSRGCGHQICADYLRTMDKAYKAYNIDLPDRQLACAPVDSQEAQDYFKAMSCAANYAWTNRQMILHWVRESFEEVYKKDSQDMNMGVVYDVAHNIAKKEKHTIKGIETEVYVHRKGATRAFGPGREEIPEEYRKIGQPVMIPGTMGTASYILKGTETAMEETFGSTAHGAGRKLSRAGAKREFNGDEVKSMLASKGITLKANSMPVVAEEAPGAYKDVDQVVNVCHKAGISLLVGKMIPIGVAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA54</accession>
    <name>F0TA54_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645355.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10004">
        <property type="protein sequence ID" value="ADZ10004"/>
        <property type="gene ID" value="Metbo_1782"/>
    </dbReference>
    <dbReference type="GeneID" id="10278239"/>
    <dbReference type="KEGG" id="mel:Metbo_1782"/>
    <dbReference type="HOGENOM" id="CLU_2327287_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="11569" checksum="3B2D88089588F1C1" modified="2011-05-03" version="1">MIANIYGQYGVNVQEVFMNIDGFLEENFDKFEDKNQILNQENLMRIKILICDLTAMEGLSPKEIKHRETLIYELISILNMYYLNNKDSLDPEIFFLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA61</accession>
    <name>F0TA61_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10011">
        <property type="protein sequence ID" value="ADZ10011"/>
        <property type="gene ID" value="Metbo_1789"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1789"/>
    <dbReference type="HOGENOM" id="CLU_3094107_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="51" mass="5851" checksum="C7027079E4E491F6" modified="2011-05-03" version="1" precursor="true">MNDSTVGAMMFVLGVVLAILAVAVPGLFYWLFWIVVIILIVYGLYQWLFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA69</accession>
    <name>F0TA69_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1797"/>
    <dbReference type="EnsemblBacteria" id="ADZ10019">
        <property type="protein sequence ID" value="ADZ10019"/>
        <property type="gene ID" value="Metbo_1797"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1797"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054151_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="241"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="35636" checksum="0AB39570D94ECD90" modified="2011-05-03" version="1">MVVNVNTTTAASVNQTNGSSSVYKVGNNNTVSNITLTDTSKSTTPISKVTVNSTNKSKINSTMAAGEPVKVNGLTLTQMKDGISRAEAFYKKNGRLPNYLSFGTRNISITTFQKNIATQGLKINTTTTTKINGLTIAQIKDGISRAEAFYKKNGRLPNYLSFGTRNISITTFQKNVSTAGLKINTSGSGNKIDTSSVSALAASLKKGSSSQYQTAVNIFNWVRDHISYSFYYNSKYGASGTLKNRIGNCCDTANLMVALARAAGIQARYLHGTCKFSSGTWYGHVWAQLYVNGKWLNADGTSYKNSLGVVKNWNTATYTLHGIYQTLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA96</accession>
    <name>F0TA96_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645397.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1824"/>
    <dbReference type="EnsemblBacteria" id="ADZ10046">
        <property type="protein sequence ID" value="ADZ10046"/>
        <property type="gene ID" value="Metbo_1824"/>
    </dbReference>
    <dbReference type="GeneID" id="10278281"/>
    <dbReference type="KEGG" id="mel:Metbo_1824"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="IQKYPAT"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="18"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="290"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="389" mass="40387" checksum="5892CAC3B360BFE1" modified="2011-05-03" version="1" precursor="true">MSFLSLVVKNPFRNKTRSALAIVGIAIGIMVIVALGMVTGGLKTSTQSTLKAGAAEITALQTGSNSFGSGGTLNESLVNDLLSTNGVKDTAGILRATNTSSSASSLSSNSSQSSFGPGGSISITGIDAGKLSLVGIDSVNGSAFSNGSTDEVIIGKTFAQDQNLTVGNTINLYGKDFKITGTFETGSFLTDAGIYMPLSTLQNLTSNDNKVSAILVKVTDNANVTTVSQNIETSYPNQLTTTTAEATASRINQGLGFIDTASWAISLLAIFIGAVGVINTMIMSVYERTREIGVLKAVGWTDKRILGMILGESIVLTLIAFVVGTIIAVVGVEILLTYSPSVSGIIKPSFALDIFARAFVVAFLVGVIGGLYPAYRASRLSPTEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA98</accession>
    <name>F0TA98_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase C60 sortase A and B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645399.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10048">
        <property type="protein sequence ID" value="ADZ10048"/>
        <property type="gene ID" value="Metbo_1826"/>
    </dbReference>
    <dbReference type="GeneID" id="10278283"/>
    <dbReference type="KEGG" id="mel:Metbo_1826"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884581_0_0_2"/>
    <dbReference type="OMA" id="WNIHTET"/>
    <dbReference type="OrthoDB" id="48740at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35753" checksum="D053F7A90714FA2B" modified="2011-05-03" version="1" precursor="true">MNIQFKHILIIIAIVVVGVAFTTVAFSGKNIKTNHFEKDEVSFDYPDSWQIVNKSRTSEIVAFNDPNSNLNVTVNRWNIVSKYKPFENFTITGPGDQSGLKFVSHKTIDLNGTKADENIYQFNESDKTYQRTEIWINKNNAMYSIIYTTTDKDLNYKSPEIQAVTKNLTIKDSSLPSTPIWGQITIPTQNVTWDIRQDGVNYYGSVFHYTDSFFTGQNGTVGLLGHHTRYSAPFANINLLNTGDKVIITDYLTQRNYIYQVESNADIKSDYKTNPIQFAPGNYELTLVTCYPPGYEEAAFQTHLKLVSIEQINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE8</accession>
    <name>F0TAE8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644251.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08900">
        <property type="protein sequence ID" value="ADZ08900"/>
        <property type="gene ID" value="Metbo_0649"/>
    </dbReference>
    <dbReference type="GeneID" id="10277094"/>
    <dbReference type="KEGG" id="mel:Metbo_0649"/>
    <dbReference type="HOGENOM" id="CLU_2340201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11433" checksum="DE241550E82ED3AF" modified="2011-05-03" version="1">MKSYTGIVIISIGIISFIITQIVNDRFSYFSMHAIGIIIVGIVWIIFNSTDSKQKYNYRETDSENNCMKCKYSDIKYFNELYANCKFLHIRVDKFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ8</accession>
    <name>F0TAJ8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1848</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645421.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1848"/>
    <dbReference type="EnsemblBacteria" id="ADZ10070">
        <property type="protein sequence ID" value="ADZ10070"/>
        <property type="gene ID" value="Metbo_1848"/>
    </dbReference>
    <dbReference type="GeneID" id="10278305"/>
    <dbReference type="KEGG" id="mel:Metbo_1848"/>
    <dbReference type="eggNOG" id="arCOG00066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_2"/>
    <dbReference type="OMA" id="CLQSANH"/>
    <dbReference type="OrthoDB" id="25049at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="8">
        <location>
            <begin position="7"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="42857" checksum="2F96E8FA64768948" modified="2011-05-03" version="1">MEVKYRYLDNSSITRMDERVLEAMKPYFFEKYAIPTSETGYSMGIEAREALEDSRNYLANYIGAGEGELIFTSGSSESSNAAIKGAAIAQKNKKGNHLIVSKIEDFPVLNSFKSLEKQGYEVSYLDVDEYGMVDPETIQKLIKPETSLVSVQHANQEIGTIQDIKTIAEICNEKNVLLHTDATHTFTKLPLDLHEIKADMVTISAHTLHGPSGIGALYLKKGTPFNKYLDGGFQEFNKRGGLENIPGAVGFKKAVELTSSEENDKFIQMRDHLMQRVLNEIPHTTLNGHPTERIPQNANITFDYVEGESITLHLDMRGFAVSTGSACFSRSLQASHVILGIGGDPERAHGSLRITFGRFNSMDDVGAMADALNEIVSKLREISAIGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL1</accession>
    <name>F0TAL1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arsenical-resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645434.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1862"/>
    <dbReference type="EnsemblBacteria" id="ADZ10083">
        <property type="protein sequence ID" value="ADZ10083"/>
        <property type="gene ID" value="Metbo_1862"/>
    </dbReference>
    <dbReference type="GeneID" id="10278320"/>
    <dbReference type="KEGG" id="mel:Metbo_1862"/>
    <dbReference type="eggNOG" id="arCOG02190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022869_0_0_2"/>
    <dbReference type="OMA" id="CTAMVLM"/>
    <dbReference type="OrthoDB" id="60543at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
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        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
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        <property type="entry name" value="PTHR43057"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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            <begin position="338"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="358" mass="39973" checksum="3D870241A2FF13DF" modified="2011-05-03" version="1">MAKDITQQVTDQICDTPKLGFLDRYLTLWIFIAMAIGVSIGYFIPTVELFINKFQVGTTNIAIAIGLILMMYPPLAKVRYEELGEVFRNTKILGLSLFQNWIIGPILMFALAIIFLHNYPEYMVGLILIGLARCIAMVIVWNELAKGDTEYAAGLVAFNSVFQVFFYSVYAWIFITVLPPIFGIYGSVVDISIVQIAESVFIYLGIPFIAGILTRYILVKRKGKEWYHETFIPKISPITLIALLFTIIVMFSLKGNYIVQIPSDVVLIAIPLLIYFVIMFFTTFWMGYKIGADYSKTSALSFTAASNNFELAIAVAVAVFGIGSGVAFAAVVGPLIEVPVLISLVNVALIFQRKYFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS7</accession>
    <name>F0TAS7_METLA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="88" mass="10061" checksum="2BF0F8EB1CA37FC6" modified="2011-05-03" version="1">MFVASLVGIFKFKELPENYGPFAQHKATMEKRTVKDDDEIAILNIAGTESFHVLFLDSYKTIAEINEELKQVDAELNYNTKKILEGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB66</accession>
    <name>F0TB66_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_0766</name>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0766"/>
    <dbReference type="EnsemblBacteria" id="ADZ09017">
        <property type="protein sequence ID" value="ADZ09017"/>
        <property type="gene ID" value="Metbo_0766"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0766"/>
    <dbReference type="eggNOG" id="arCOG10607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086991_0_0_2"/>
    <dbReference type="OMA" id="FFTTAAW"/>
    <dbReference type="OrthoDB" id="56913at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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    </feature>
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        <location>
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            <end position="130"/>
        </location>
    </feature>
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        <location>
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            <end position="179"/>
        </location>
    </feature>
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        <location>
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            <end position="221"/>
        </location>
    </feature>
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        <location>
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            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="292" mass="33037" checksum="32EA1C4C47D18D67" modified="2011-05-03" version="1">MDGNILNKKFDECPDILYHKMPKGWVCREIIETGPFITGALIISPEGLEYKWRSRFHRKHHNLLDSGTVSTWFAPGAIAWWIGVFFAIGSIFFALGSAPPYINWAGPHLDAMTYFLGSIFFTTAAFLQYIETVNVRRVPKSMNIVDEIKIFTWEPRRIDWVASVVQFIGTLFFNFSTFFAVNMLLTVQQINHQVWVPDIYGSICFLVASSLVWIEVGHGLVSFNWKNISWQIAILNLIGSVAFGISAIYAFTLPSTGEPVNFAIQNWGTFVGGVCFFIAAVLLLPERTMEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB82</accession>
    <name>F0TB82_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644384.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0782"/>
    <dbReference type="EnsemblBacteria" id="ADZ09033">
        <property type="protein sequence ID" value="ADZ09033"/>
        <property type="gene ID" value="Metbo_0782"/>
    </dbReference>
    <dbReference type="GeneID" id="10277230"/>
    <dbReference type="KEGG" id="mel:Metbo_0782"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OMA" id="TIRDSWI"/>
    <dbReference type="OrthoDB" id="118816at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="SSF55159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="111" mass="12788" checksum="31C220AF6DB3B609" modified="2011-05-03" version="1">MKVCEICGLPEELCVCEEIAREIQKVKVYTVRRRFGKLMTIVEGIDEHDIDIRELTKELKAKCACGGTAKKGQIELQGDHKRRVKEVLAELGFSSDTIEIRDRDDRGRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB2</accession>
    <name>F0TBB2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpo10</name>
        <name evidence="2" type="synonym">rpoN</name>
        <name evidence="3" type="ordered locus">Metbo_0812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644414.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0812"/>
    <dbReference type="EnsemblBacteria" id="ADZ09063">
        <property type="protein sequence ID" value="ADZ09063"/>
        <property type="gene ID" value="Metbo_0812"/>
    </dbReference>
    <dbReference type="GeneID" id="10277261"/>
    <dbReference type="KEGG" id="mel:Metbo_0812"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_1_1_2"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="OrthoDB" id="123859at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="PTHR23431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="SSF46924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="55" mass="6351" checksum="46506C22D2C98348" modified="2011-05-03" version="1">MIPVRCISCGKVVSAYFDEYQKRTAEGEDPKKVLDDLGVTKYCCRRMLIAHVEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD6</accession>
    <name>F0TBD6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BPL_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1985"/>
    <dbReference type="EnsemblBacteria" id="ADZ10205">
        <property type="protein sequence ID" value="ADZ10205"/>
        <property type="gene ID" value="Metbo_1985"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1985"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057558_2_0_2"/>
    <dbReference type="OMA" id="EIANARC"/>
    <dbReference type="OrthoDB" id="96307at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL_N" evidence="1">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="240" mass="25905" checksum="2595305343A485DB" modified="2011-05-03" version="1">MCIIGIVLFGYSTIKTSSSTSNSVKYVNVLIYDGTGTMSSSVSGLENCLDELNNKSSNKKFIYSTAVEINSQTLQGYDVLIMPGGDASEYIQNSKIDNSSIKQFVSSGKGYLGICAGAFAASNYVDGYYNGWGLEPDVTAETVDYVGDLSISTTSYGKKLSGTSDVTIYHQNGPALYTNDTEHILSTFSDNNTGYEGYADMVGESYGSGRVLLSGSHPELEPENQELLMNMLLWLSKNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI3</accession>
    <name>F0TBI3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="8">DGGGPL reductase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Geranylgeranyl reductase</fullName>
            <shortName evidence="8">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645603.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2033"/>
    <dbReference type="EnsemblBacteria" id="ADZ10252">
        <property type="protein sequence ID" value="ADZ10252"/>
        <property type="gene ID" value="Metbo_2033"/>
    </dbReference>
    <dbReference type="GeneID" id="10278494"/>
    <dbReference type="KEGG" id="mel:Metbo_2033"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OMA" id="SLCDPFL"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="9">
        <location>
            <begin position="5"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="209"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="290"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="294"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="395" mass="42646" checksum="1BE026739DBBC116" modified="2011-05-03" version="1">MKLIETDVLVIGAGPAGSSTAKYAAQNGAKVILMDKKSEIGAPKRCAEGVSKEGLKKLGIEPSSRWVTKELSGVRLVSPNGTDVWLNDDQVKLPEAGYILERKVFDKFMAMDAARAGATIMIKTLARGMRKDSNGYIVSCENMGEDFEIKAKIVVGADGPESRVGRWGGLKTVVKPKNMESGIQFEMAGVEMEDPDCIEFYFGSVAPGGYAWIFPKGGDIANVGLGIVNTVTDKSAYQHLLEFVENCPATQNAQAVELNIGGDPVGGMLKDLVEDNVMIVGDAAGHVNPLTGGGIITALEAGKYAGEVAAEAIKEGDCSKKRLKEYEKICKEEIGDSFNKYFKTKEYMLSLSDEELDSIAEAFQDYEFEKISTSELVKLLIKVSPKALLKLGKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK5</accession>
    <name>F0TBK5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MurG-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645625.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2055"/>
    <dbReference type="EnsemblBacteria" id="ADZ10274">
        <property type="protein sequence ID" value="ADZ10274"/>
        <property type="gene ID" value="Metbo_2055"/>
    </dbReference>
    <dbReference type="GeneID" id="10278517"/>
    <dbReference type="KEGG" id="mel:Metbo_2055"/>
    <dbReference type="eggNOG" id="arCOG07615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796003_0_0_2"/>
    <dbReference type="OMA" id="PESNLFQ"/>
    <dbReference type="OrthoDB" id="67187at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="37914" checksum="429283BCE7E277BF" modified="2011-05-03" version="1">MKALFVVTGRGIGGDAVTALNIAKALEKYGFQCEFALDPTAPGLLFKKHGIEWHKTSIPQAGGHAATKKTLVNAGFKTLKASIKTASLCRKIKPDVVVGVIGGGAVVGCLGAMIAKVPSVGILITPADAKVCTKITTTVVLPESNLFQMDLNNETIHKAYSPVSPDIVVGDRENALKNLPDNFDENLPTILLSSGSTLFEKMAKAASMLGDKNLKANLIVVGHPLEDEYESYLENPNLINLGYIDWIKDLYKVVDIAVVTDDGMMIHEAMAWGIPVVALLGVKYGRYHNLAAVFEGAVLESELENLDTVIDEAFANITTMKQTAKNYGRDVLESSDKIAGIISKRAGGPSSSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP0</accession>
    <name>F0TBP0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644468.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0868"/>
    <dbReference type="EnsemblBacteria" id="ADZ09117">
        <property type="protein sequence ID" value="ADZ09117"/>
        <property type="gene ID" value="Metbo_0868"/>
    </dbReference>
    <dbReference type="GeneID" id="10277317"/>
    <dbReference type="KEGG" id="mel:Metbo_0868"/>
    <dbReference type="eggNOG" id="arCOG01744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329469_0_0_2"/>
    <dbReference type="OrthoDB" id="90412at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="209" mass="23495" checksum="FEDAD97706990FA7" modified="2011-05-03" version="1">MKSYTHIAGALVLFLSFAILKNLNQIFLGVFLAGWISVFPDLLDKILGKHKSYGHSIFWIVPTIIIGYFNFTVGAALTIGLISHSALDVMTTYGTPIFSPLWNTNFVVFNGKRRVKTGTNQDKAFSLTMIFLLIPMMFYLTIPVQNTLTNTILPVNDIYHNNTNTSGVNMEKNNININIQSNHEKNKTIIVDKINETETTIKIKTEPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS8</accession>
    <name>F0TBS8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644506.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0906"/>
    <dbReference type="EnsemblBacteria" id="ADZ09155">
        <property type="protein sequence ID" value="ADZ09155"/>
        <property type="gene ID" value="Metbo_0906"/>
    </dbReference>
    <dbReference type="GeneID" id="24964523"/>
    <dbReference type="KEGG" id="mel:Metbo_0906"/>
    <dbReference type="eggNOG" id="arCOG00385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640324_0_0_2"/>
    <dbReference type="OrthoDB" id="136474at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="428" mass="50189" checksum="2ECD697F15E2B24D" modified="2011-05-03" version="1">MIRKYIKNKIINSNLYSYLTDRNRDLQVQILELQEENKDLKLKVKQFYESRSTIETNISVVKTENMELKKWIDHQKQSRKELENELQKWKQDNSDLKKSIKTHETAESNLQIQLSRLKFLNTQLLKEYQFSMHDTVQLNGNKTVNPAIDEFKSSLKLINENNDLFTLWISTDHTLPEIQHLSIQSMILTGHTVTLYTYEDLDNVPDGVKLKDANQIIDESEIFTYKEGFNKGSYSGFANVFRYKCIEITGKSWFDCDILAIKNINEIYPLENIISSQYNVDNTIYPNNGFLRLSRGDELIINLINAVDSMDLENVKHGETGPRLLKSVMDSENPEHYKYLADPNFVSPINYFEYEDYLKPSKQIIPTLDLDDIWGFHIWNAMFRENGHQNEKTKGGFYHDLKQIILSSNNPEEYSEELKKFLGKFSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT8</accession>
    <name>F0TBT8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2061"/>
    <dbReference type="EnsemblBacteria" id="ADZ10280">
        <property type="protein sequence ID" value="ADZ10280"/>
        <property type="gene ID" value="Metbo_2061"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2061"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="156"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="230"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="277" mass="31736" checksum="70AE139C4F8E4D3E" modified="2011-05-03" version="1">MPDKILIVEDEVITAMDIKNMLKNYGFDVLGIASTGKDAINKAEEFRPDLVLMDISLKGDMDGIEAAEEIKSHYNIPVVYMSAFTDTNTFERIKFTNPYGFVNKPVSSELLLISIETAIYKHNLDKKLAESQEKLKDAHDNLELRVQERTAELENAYKELSDIYNNAPCGYHSLDKDGYFVKINNTELKWLGYTKDEIIGKKKFRDLVTDEGVEKFEMNYPGFLEQGFVHDLEFDMIKKDGSPLTILLSATAIYDDNHNFLISRSTIFDISNRKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV9</accession>
    <name>F0TBV9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645652.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10301">
        <property type="protein sequence ID" value="ADZ10301"/>
        <property type="gene ID" value="Metbo_2082"/>
    </dbReference>
    <dbReference type="GeneID" id="10278544"/>
    <dbReference type="KEGG" id="mel:Metbo_2082"/>
    <dbReference type="HOGENOM" id="CLU_1736431_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="16678" checksum="1780376BFFB7B769" modified="2011-05-03" version="1" precursor="true">MKMRIMSIAMFFVVLMMSFSAVSAAEYNSFTNVNMINSGALNVNTEAHDNATMALHGDLSFKGIQYVDYAAVKAKWTDFVPASETYFIILTINYVDTFGDIADYYEYLYVTNGTSSTSDVMAIHYMDLSKPANVTIEAEKVSYEPCGPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX2</accession>
    <name>F0TBX2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2095"/>
    <dbReference type="EnsemblBacteria" id="ADZ10314">
        <property type="protein sequence ID" value="ADZ10314"/>
        <property type="gene ID" value="Metbo_2095"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2095"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="OMA" id="PMITKNI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="20043" checksum="65ED0F2249187A40" modified="2011-05-03" version="1">MNYYECYHGDKMKQQEMDRLVDSLLIYMPMFYKKLTTAKETDITKSSYKKRTADQYQILGLLEHYNHLPISEIGKRLLLSRPNMTAHLDKLVSEGMVERIPDETDRRVINIGITQNGLDYIKVSRTWVKNNIKETLNVLNNEELVELEHCIETIKSKILMIEGCHHGTIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC23</accession>
    <name>F0TC23_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09174">
        <property type="protein sequence ID" value="ADZ09174"/>
        <property type="gene ID" value="Metbo_0926"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0926"/>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644988_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="425" mass="47256" checksum="8E85A9BABA907D94" modified="2011-05-03" version="1">MTTNISNILLKGLLSSLRIDKLNLLYLGRDFIRLKLVLPVLMLCMIFFTVQGTMDHTSAASTNQTTVSKTNQTVTSAATLKTTTKSTETKLVSSTKKVYFKPAQIRAAATTVKNSILINHKLPSYVTIGSTKVKMPQFLELLTTAIIKINYKDSTSILLKSVTNTTGTETIKTSNFNKTAYLKAAKTLKNYMDTYHKVPGVYKTSNGRMDYSNLIYTYCKIIDFYNVQNRLPNYVSVTTFKKVIALRPVYITSDNIINKTADNARINSIVKGLRALGLYAVNWGLGPNSHVAVLGQTNLPKNALIVDIYGGACAGTLYEMGLDWYKNLRASKKVYTIFWPPSTQITGLDFLPRAHDDNFTPKYGEANGFPDQVDVNKDGIFETGLPGREDGLAHPDQYLHSNGYQYLYSGDISTIVNAIYKQTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC24</accession>
    <name>F0TC24_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0927"/>
    <dbReference type="EnsemblBacteria" id="ADZ09175">
        <property type="protein sequence ID" value="ADZ09175"/>
        <property type="gene ID" value="Metbo_0927"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0927"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592668_0_0_2"/>
    <dbReference type="OrthoDB" id="133539at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="372"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="50429" checksum="71B3713DD7649BA5" modified="2011-05-03" version="1">MDLGVARIKKKSLIIFLLIIGVGLLVNFNVSSAASIQDTELTLNTSSTLNNTNDQVVTPKTLKASNLVSKTVGESPAYQSAGDADVSVRSSASNSSPNYLRNVVLVVIVKNNGPNNVSNINLALKLNKTYLKWISDDSKGLYNPNTGIWSINNLTTGNRTVLHVVEQVLTYDRTVTVMSNFTSGSFTDTDPTNNFSILNLTIPRASDLKVTQSTAKYNVKYLHTFVVAVTVKNSGPSTAKNVVVNCGLDPNVYAPVSYSQNRSYNPSTGNWTVAYLKSGSSITLHIFVRMLAFNSVVSNMVAVSSDTYDFNKSQNRAKMLITTPALTVNSLASSLKIGTSSRYQQAVNIVNWVRDNIVYSFYYNTKYGASGTLQRLKGNCADTAHLVVALARVSGFSARYKHGTCHFIESGHWYGHVWANIYVNGKWYSADATGYKNTLGVIKNWDTSTFTLHGTYNTLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC34</accession>
    <name>F0TC34_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644536.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09185">
        <property type="protein sequence ID" value="ADZ09185"/>
        <property type="gene ID" value="Metbo_0938"/>
    </dbReference>
    <dbReference type="GeneID" id="10277387"/>
    <dbReference type="KEGG" id="mel:Metbo_0938"/>
    <dbReference type="eggNOG" id="arCOG09716">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131314_0_0_2"/>
    <dbReference type="OMA" id="NANTHIS"/>
    <dbReference type="OrthoDB" id="102911at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="18140" checksum="1243D5A59568B898" modified="2011-05-03" version="1" precursor="true">MKNKIVILVVMTIVILFSGCIQSNTSQIDALASTINNHIKNGDQYYNNSGQAVNQFQYSTALSYCNNASSEFNLAQTSAKQGLTYAQNSNDTVYVNYMQDVVNELDAKINATEELKTAISYLQDNDSVNANIHIGNANDLMNNAVQFNNLRQQIIKQNPTKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC43</accession>
    <name>F0TC43_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0947"/>
    <dbReference type="EnsemblBacteria" id="ADZ09194">
        <property type="protein sequence ID" value="ADZ09194"/>
        <property type="gene ID" value="Metbo_0947"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0947"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_394149_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="596"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="699" mass="76795" checksum="84113E415DACAB55" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC44</accession>
    <name>F0TC44_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">M6 family metalloprotease domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0948"/>
    <dbReference type="EnsemblBacteria" id="ADZ09195">
        <property type="protein sequence ID" value="ADZ09195"/>
        <property type="gene ID" value="Metbo_0948"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0948"/>
    <dbReference type="eggNOG" id="ENOG502N59E">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_788968_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03296">
        <property type="entry name" value="M6dom_TIGR03296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="38974" checksum="28DFDFEFDDA3FE5D" modified="2011-05-03" version="1">MMFAGILAFSQVSYALDPPSKQQINNYKKDGTYSARIANATKLENNQFDPVLVEKFNKKHGIKTVSSQTSKIKSVGSSSGTSGNSYSNVPINWRGGLPSTGNVKVPVILIDFPDYLHGVNETVAQADSKFFGNENSNNYPYESLRNYYLRSSYGKLNITGTIYGWYTASHIRSYYSNLSSSGNFGIGNEALIREAVNYYHNHGANFTQFDNDNNGYIDSLFVKWTGPDNGWANFWWAYQSNLESNSSLTVDGKKFNKFVWSWYSNTAQASPTDSIRTDIHETGHLLGLPDYYDYDESTGPDGGVGGLDMMDSNWGDHNCFSKYILGWINPQVVSSGTKKIALNPSGTSNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC93</accession>
    <name>F0TC93_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645711.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2143"/>
    <dbReference type="EnsemblBacteria" id="ADZ10360">
        <property type="protein sequence ID" value="ADZ10360"/>
        <property type="gene ID" value="Metbo_2143"/>
    </dbReference>
    <dbReference type="GeneID" id="10278606"/>
    <dbReference type="KEGG" id="mel:Metbo_2143"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072493_0_1_2"/>
    <dbReference type="OMA" id="CGYTDNP"/>
    <dbReference type="OrthoDB" id="72347at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="30035" checksum="014CF77DFA3B023D" modified="2011-05-03" version="1">MPIKQATINALKILGLTDYESKAYLSLASLISATALEISEDSGVPLSRTYDVLKSLYKKGFLEITRGKPLIYGVVPPQDVFEKSRVKIKEELDEAESEVINIYESQISKSPAPIWLIYGSDKIVKKELEIIGRAKSTLHIASGFMFQEEVEKLNEALNKSLKRGVQTRIIAAPYNITDDEKIDISENIKKLDCEIKTFQIPFIKAIIRDKKEMLLIFCKFKDGSVISQTAIGVWNQYTEFVETITDLYNLVWTMNLFNMATDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB5</accession>
    <name>F0TCB5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RDD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2167"/>
    <dbReference type="EnsemblBacteria" id="ADZ10382">
        <property type="protein sequence ID" value="ADZ10382"/>
        <property type="gene ID" value="Metbo_2167"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2167"/>
    <dbReference type="eggNOG" id="arCOG03633">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155013_0_0_2"/>
    <dbReference type="OMA" id="VSAFMWI"/>
    <dbReference type="OrthoDB" id="118254at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16604" checksum="0245826D0FA16DB2" modified="2011-05-03" version="1">MGNFWGRRLIALIVDIIILTLFMWILSGIVFLITAGIGVFSTLTYWIFIGAIIILVYFTYFEGKTGKTFGKRLFNLKVVAEDGNLTYKKSLIRSLSKILYLPLILDLILGFIFVKSNDRFLDRVSGTQVVPADQESQQRNDLSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC0</accession>
    <name>F0TCC0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2172</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645738.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2172"/>
    <dbReference type="EnsemblBacteria" id="ADZ10387">
        <property type="protein sequence ID" value="ADZ10387"/>
        <property type="gene ID" value="Metbo_2172"/>
    </dbReference>
    <dbReference type="GeneID" id="10278635"/>
    <dbReference type="KEGG" id="mel:Metbo_2172"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="OMA" id="TYHNVKF"/>
    <dbReference type="OrthoDB" id="93004at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="25578" checksum="12BAD99306DB8EE4" modified="2011-05-03" version="1">MIKAVFLDIDDTIYNTSEFAKLARRAALNAMIDAGLPMSHEDAYKLLREIIKEKGSNYNKHLNVLTKRVFGEEKPLLIAIGMITYHNVKFALLRLFPNTMPTLIDLKSKGYKLGVISNGLTIKQYEKLVRLGLHHFFDFVITSEEAGVEKPDVKIYQLAMERMGCDPENSVMIGNNFRDDILGAIDAGMSAIYLTPGLTEQERKTIKEREIDLDIVSDIGEIRKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG3</accession>
    <name>F0TCG3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644591.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0993"/>
    <dbReference type="EnsemblBacteria" id="ADZ09240">
        <property type="protein sequence ID" value="ADZ09240"/>
        <property type="gene ID" value="Metbo_0993"/>
    </dbReference>
    <dbReference type="GeneID" id="10277442"/>
    <dbReference type="KEGG" id="mel:Metbo_0993"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136066_0_0_2"/>
    <dbReference type="OMA" id="LADIWEK"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="190"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="255" mass="28985" checksum="57CFD8123552D860" modified="2011-05-03" version="1">MVQKEPLKDMQIELFSTSEGIYAINSPVRVKILSVLRKGELSFDQLVELSGKAKSTVSVHLRRMVDEGIIGSKVDENDARKKIFFIKSEYLGHLSGKKVISENFDEYVNKYIESEGDPFEFFRLIFHTLRVSLIRQGIDIDPILYEAGVQVGQTLYQKVKDPDIQVLAQNIAKFWQGHSLGTVEVIKLEPLTINVYDCFECQNLPPLGKAACAFDSGILKALFTAHFQEEQIVNETRCYALGDDHCSFTIKSREW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI3</accession>
    <name>F0TCI3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644611.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1013"/>
    <dbReference type="EnsemblBacteria" id="ADZ09260">
        <property type="protein sequence ID" value="ADZ09260"/>
        <property type="gene ID" value="Metbo_1013"/>
    </dbReference>
    <dbReference type="GeneID" id="10277462"/>
    <dbReference type="KEGG" id="mel:Metbo_1013"/>
    <dbReference type="eggNOG" id="arCOG10173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202625_0_0_2"/>
    <dbReference type="OMA" id="RDMRFYQ"/>
    <dbReference type="OrthoDB" id="75707at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="24481" checksum="FCAF95D11C792607" modified="2011-05-03" version="1" precursor="true">MNFRLVLLIFLVVLFAAGTGYLSYSQSTGLPLKEAYDNGNVVITQNTSAGTVPHQVVVVNNGRDPVKVQVGDVLTANSSQDLVVAENKTVSTNSSETIYAYCLDPSTQAVVGSKLKANGTASNAIKQVIDGSNIHDLNNATNAQLELWILTAGVNFNIYSGEPVALVNTQKITYTTLKQMVTNSKSNLASQFNVKVDNIKNINQNSTQSSDGTLKGVYHWFKSMIGMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL6</accession>
    <name>F0TCL6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644644.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1046"/>
    <dbReference type="EnsemblBacteria" id="ADZ09293">
        <property type="protein sequence ID" value="ADZ09293"/>
        <property type="gene ID" value="Metbo_1046"/>
    </dbReference>
    <dbReference type="GeneID" id="10277495"/>
    <dbReference type="KEGG" id="mel:Metbo_1046"/>
    <dbReference type="eggNOG" id="arCOG00519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068890_3_1_2"/>
    <dbReference type="OMA" id="VFCTAGK"/>
    <dbReference type="OrthoDB" id="63381at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="155" mass="16910" checksum="4EDE9B8A915308C9" modified="2011-05-03" version="1">MKTLIVYYSRTGVTKGIAEEISHSLDCDIEEILDKENRSGIIGYIKSGYETVRNKVSEIEPPKNDLSNYELLIIGTPVWAGKMAVPVKTYIEQNRDKIPNLACFCTCGGTGIDGTLNGIAESAQVTPLASFGLKAPELKNGSYSSTIEKFIENIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ3</accession>
    <name>F0TCQ3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-adenosylhomocysteine deaminase</fullName>
            <ecNumber evidence="2">3.5.4.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.28" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645794.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2229"/>
    <dbReference type="EnsemblBacteria" id="ADZ10443">
        <property type="protein sequence ID" value="ADZ10443"/>
        <property type="gene ID" value="Metbo_2229"/>
    </dbReference>
    <dbReference type="GeneID" id="10278695"/>
    <dbReference type="KEGG" id="mel:Metbo_2229"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="OMA" id="TDNFMAN"/>
    <dbReference type="OrthoDB" id="75040at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="50"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="383" mass="41452" checksum="5A1EB939D90FF9F9" modified="2011-05-03" version="1">MITIKNAKVLCREAMELQKLNIIIQDNEIVELTPRGSKGRVVDGQNCVAAPSLINSHVHIGDSVAKDVGDGEPIEKIVKPPNGIKHRILGETSSKEMMNSMRNSMEEMLQTGTTTFVDFREGGVEGIEILEEASKNIPIRKIVLGRHASFQESDDLTQIKMAAKEILNHCDGIGLSGFGEIKDDVARLIVETCDEVGKLSAIHVAEYLKLQSESLNVHGKTEIQRAVDAGFNLLIHMTEPVDHDLETVGRSNATVVSCPRSNGALSVGIPPIKAMLDNGVNVALGTDNLMFNSPNMFSEMEYALKVTRGIYKTHLDPLEIFKMATVNPAKALGLEAGVIEEGKIADIMLIKGVSGDPILSIINRTRSKGINTLIKDGSVVLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T641</accession>
    <name>F0T641_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H/ACA RNA-protein complex component Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2335"/>
    <dbReference type="EnsemblBacteria" id="ADZ10548">
        <property type="protein sequence ID" value="ADZ10548"/>
        <property type="gene ID" value="Metbo_2335"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2335"/>
    <dbReference type="eggNOG" id="arCOG02466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165884_2_0_2"/>
    <dbReference type="OMA" id="HYAKQGF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="31"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="11056" checksum="DEB54C780EABA692" modified="2011-05-03" version="1">MGRQMKQLGKISHISNRNRIIIKSNQTPGFGLPVYTEDNKKFGTVYDVFGPTKGQYISVKVFAKNSRNLEKLIGKDLYIPSSNKIEKRGRRKRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T654</accession>
    <name>F0T654_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ordered locus">Metbo_2348</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645912.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="SMR" id="F0T654"/>
    <dbReference type="STRING" id="877455.Metbo_2348"/>
    <dbReference type="EnsemblBacteria" id="ADZ10561">
        <property type="protein sequence ID" value="ADZ10561"/>
        <property type="gene ID" value="Metbo_2348"/>
    </dbReference>
    <dbReference type="GeneID" id="10278814"/>
    <dbReference type="KEGG" id="mel:Metbo_2348"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OMA" id="QIMDMET"/>
    <dbReference type="OrthoDB" id="98221at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="Transl_elong_IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="PTHR11673"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eIF-5a" evidence="12">
        <location>
            <begin position="69"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="131" mass="14405" checksum="BE20C15C1E17F7A5" modified="2011-05-03" version="1">MSKKVVEVKTLKVGKYVIINDEASKITSISTSSPGKHGAAKARVEAVGIFDNQKRTFVKPVDAKCDVPMIDKNLGQVLAIMGNDVQLMDMETYETFEIEIPEELKGKINEGAEVDYIVAMGNKKLMRIKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T676</accession>
    <name>F0T676_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644742.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09391">
        <property type="protein sequence ID" value="ADZ09391"/>
        <property type="gene ID" value="Metbo_1147"/>
    </dbReference>
    <dbReference type="GeneID" id="24964538"/>
    <dbReference type="KEGG" id="mel:Metbo_1147"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754712_0_0_2"/>
    <dbReference type="OrthoDB" id="115137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="16457" checksum="CECB77DD2A459230" modified="2011-05-03" version="1">MSANSVGAYNVTATHDYVKATAICSCGENTYSSGNNNYIYHTSTFKNYCPRCKSYGTLKFNPKGVPEGEWTCTKCSSDYCAADGREKITGSHYSLVEYNPPQVKVQSKVNSGLNIEMNVITDLNNVNNKSILEADYQRLRNSNYFAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T678</accession>
    <name>F0T678_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644744.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1149"/>
    <dbReference type="EnsemblBacteria" id="ADZ09393">
        <property type="protein sequence ID" value="ADZ09393"/>
        <property type="gene ID" value="Metbo_1149"/>
    </dbReference>
    <dbReference type="GeneID" id="10277599"/>
    <dbReference type="KEGG" id="mel:Metbo_1149"/>
    <dbReference type="eggNOG" id="arCOG10251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200896_0_0_2"/>
    <dbReference type="OMA" id="INTWGIY"/>
    <dbReference type="OrthoDB" id="131367at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8191" checksum="BFA9DE4D1A4C5DFB" modified="2011-05-03" version="1">MSDNNKTIDRDLPVKKRKINTWGIYISLIFIVVGLVWYCVNIGLIPVSLIQEQAGPIVVIIVGVLILIKSVMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F4</accession>
    <name>F0T6F4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6A</name>
        <name evidence="9" type="ordered locus">Metbo_2375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645939.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2375"/>
    <dbReference type="EnsemblBacteria" id="ADZ10588">
        <property type="protein sequence ID" value="ADZ10588"/>
        <property type="gene ID" value="Metbo_2375"/>
    </dbReference>
    <dbReference type="GeneID" id="10278841"/>
    <dbReference type="KEGG" id="mel:Metbo_2375"/>
    <dbReference type="eggNOG" id="arCOG04143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="OMA" id="NWGEARF"/>
    <dbReference type="OrthoDB" id="22842at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="PTHR10848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="SSF56726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TP6A_N" evidence="8">
        <location>
            <begin position="75"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="358" mass="40631" checksum="8CC2F356A2445B31" modified="2011-05-03" version="1">MTSRKEHALKRRDLAVNKLTSLGDRIIDDVAKQTVPAIRVPSRGTSNIVYDEDRRYYVLGDRYGQRSLGNVKQIKKIGQMVYMANFCKGLVQTGKTATLREMYYVSEGWDIDFGDQQESNIVGEDLEVTLGITREDLGLMPEEDGASVYGDITLKDDDVEINAMRMGKSGYTISPTIDEVELLDHNVERVIAVETMGMFHRMVQENAYKKFNTLIVGLKGQAARATRRFLKRVNEELGLPVYICNDGDPWGFHIAMVIISGSAKLAHVNHELATPNAKFLGVTASDIVNYDLPTDPLKDIDVLRLKELYKDPRYRDEFWKVEIKKMLKIGKKAEQQSFSKYGLEYVVDTYIPEKLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F8</accession>
    <name>F0T6F8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="10">GAPDH</shortName>
            <ecNumber evidence="5 10 12">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gap</name>
        <name evidence="14" type="ordered locus">Metbo_2379</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10 12">
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="5 10 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645943.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2379"/>
    <dbReference type="EnsemblBacteria" id="ADZ10592">
        <property type="protein sequence ID" value="ADZ10592"/>
        <property type="gene ID" value="Metbo_2379"/>
    </dbReference>
    <dbReference type="GeneID" id="10278845"/>
    <dbReference type="KEGG" id="mel:Metbo_2379"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OMA" id="NAIVPNP"/>
    <dbReference type="OrthoDB" id="32079at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="7 10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="13">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="338" mass="36700" checksum="1A1CD6B66E67F015" modified="2011-05-03" version="1">MKAVGINGYGTIGKRVADAVSAQDDMKIVGVTKRTPNFEAKRAVELGYDIYMSAPDRENLFEEADIPVKGTINDLYDKVDVIVDCTPGGVGAKNMADYEKAGVKGIFQGGEKHDQIGRSFNSFSNFKESVGADFTRVVSCNTTGLCRTLNPIKELAGINKVRAVMVRRGADPGQVNKGPINSIVPNPPTVPSHHGPDVQTVMKGLKITTMAILVPTTLMHQHNLMVELDSSVSVDDVLDVLEKTPRVIPVEASSGLGSTAEIMEFAKDLGRPRGDLFEIAVWKESLNIVDNELFYMQAIHQESDVVPENVDAIRAMLEMETDPQKSIAKTNKNMGIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L4</accession>
    <name>F0T6L4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coenzyme F420:L-glutamate ligase</fullName>
            <ecNumber evidence="8">6.3.2.31</ecNumber>
            <ecNumber evidence="8">6.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coenzyme F420-0:L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Coenzyme F420-1:gamma-L-glutamate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cofE</name>
        <name evidence="10" type="ordered locus">Metbo_2435</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="8">Monovalent cation. The ion could be potassium.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CofE family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.31" evidence="8"/>
    <dbReference type="EC" id="6.3.2.34" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645999.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2435"/>
    <dbReference type="EnsemblBacteria" id="ADZ10648">
        <property type="protein sequence ID" value="ADZ10648"/>
        <property type="gene ID" value="Metbo_2435"/>
    </dbReference>
    <dbReference type="GeneID" id="10278907"/>
    <dbReference type="KEGG" id="mel:Metbo_2435"/>
    <dbReference type="eggNOG" id="arCOG02714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051152_1_1_2"/>
    <dbReference type="OMA" id="KHGFVCA"/>
    <dbReference type="OrthoDB" id="73785at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052619">
        <property type="term" value="F:coenzyme F420-1:gamma-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01258">
        <property type="entry name" value="F420_ligase_CofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023659">
        <property type="entry name" value="F420_ligase_CofE_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0630">Potassium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_ligase" evidence="9">
        <location>
            <begin position="11"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="27184" checksum="8CAC8FC04BB7118B" modified="2011-05-03" version="1">MCVKLIGIKNIPMIGQGDDVGTIIIEAAKSEGLEIMDHDVFVVAETVVSKAEGNLIKLDSIEVSEQALEISEKTSKDPRIVQTIIDESVEIIKVGPNFIISETKHGFVCANAGIDESNVETGFLKPIPHDSDESASIIRTTIESATGKNVAVILSDTQGRPFREGAVGVALGVSGIDPVWNRQGETDLFERELETTTIAVADELASAASIVMGQAAEGLPVIIVRGVEYAEKLRSTTSNIKPILRPKKFDVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N3</accession>
    <name>F0T6N3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase domain protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643617.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0013"/>
    <dbReference type="EnsemblBacteria" id="ADZ08266">
        <property type="protein sequence ID" value="ADZ08266"/>
        <property type="gene ID" value="Metbo_0013"/>
    </dbReference>
    <dbReference type="GeneID" id="10276435"/>
    <dbReference type="KEGG" id="mel:Metbo_0013"/>
    <dbReference type="eggNOG" id="arCOG02292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_18_0_2"/>
    <dbReference type="OMA" id="EGSGRHK"/>
    <dbReference type="OrthoDB" id="88508at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="25164" checksum="0AB289A6837E2DC6" modified="2011-05-03" version="1">MDRLALFDIDKTLIVGSSCHYNALKKSLTQVYGVENPQSLKNMQGMTDLKIICTTLKNENIDVNTIKSGLEECMIVMHDNFNEALKMGDLKVLKGVKPLLQDMQEHQIPMGLVTGNMEAIAWLKLDKVGLKQYFQFGGFGDRVLKRSGLVKKALEASKNIIGNIDRKHVYLIGDTPRDIWGGQKLNVNTLGVATGDFSEHDLRAAGADYVVKDLTDKDRILSIILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U4</accession>
    <name>F0T6U4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1222</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644815.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1222"/>
    <dbReference type="EnsemblBacteria" id="ADZ09464">
        <property type="protein sequence ID" value="ADZ09464"/>
        <property type="gene ID" value="Metbo_1222"/>
    </dbReference>
    <dbReference type="GeneID" id="24964545"/>
    <dbReference type="KEGG" id="mel:Metbo_1222"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_2"/>
    <dbReference type="OMA" id="FAKFMDP"/>
    <dbReference type="OrthoDB" id="35407at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="20"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Abhydrolase_4" evidence="4">
        <location>
            <begin position="178"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="240" mass="27049" checksum="61C625D1476F60AF" modified="2011-05-03" version="1">MYANVNGIKIYYEIFGEGKPLMIIWGMGGEIGSFVDKLKETKNYKLITFDNRGTGRTDKPNDEYTIEIMAEDAISLLDELKIQKVSILGISMGSRIAITMAAKYPDRVSNLILNVAAAKSAQNSDENSKEAYEKLKQVANNPKLLNAMGKYPPSSTSFLRLFDALTSYDGTKYLKEIKSPTLIVNGTQDSSTPIKYAKELFDRIQNSKLIMAKEDHFFIRTKPELLIDPMNKFIFKKNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V2</accession>
    <name>F0T6V2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644823.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09472">
        <property type="protein sequence ID" value="ADZ09472"/>
        <property type="gene ID" value="Metbo_1230"/>
    </dbReference>
    <dbReference type="GeneID" id="10277681"/>
    <dbReference type="KEGG" id="mel:Metbo_1230"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184949_0_0_2"/>
    <dbReference type="OrthoDB" id="113119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="75" mass="8284" checksum="2A37BCA44E9BEAB0" modified="2011-05-03" version="1">MKIKDEIIGKEVMDNQGALVGKIKDLDLDLKSKKIDAIELEDTGLSGKIGLGEKKILPFDLVEHIGDKVIIKQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W5</accession>
    <name>F0T6W5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="8">SHMT</shortName>
            <shortName evidence="8">Serine methylase</shortName>
            <ecNumber evidence="8">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyA</name>
        <name evidence="11" type="ordered locus">Metbo_1243</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible interconversion of serine and glycine with tetrahydromethanopterin (H4MPT) serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methylenetetrahydromethanopterin + glycine + H2O = 5,6,7,8-tetrahydromethanopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:47104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644836.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1243"/>
    <dbReference type="EnsemblBacteria" id="ADZ09485">
        <property type="protein sequence ID" value="ADZ09485"/>
        <property type="gene ID" value="Metbo_1243"/>
    </dbReference>
    <dbReference type="GeneID" id="10277694"/>
    <dbReference type="KEGG" id="mel:Metbo_1243"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OMA" id="RCQHSEV"/>
    <dbReference type="OrthoDB" id="22518at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="10">
        <location>
            <begin position="8"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8 9">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="421" mass="47017" checksum="BC1FA588D02A7144" modified="2011-05-03" version="1">MENKNYIEQIREYTKDHQNWMKNSINLIASENITSKPIKEVLASDLSHRYAEGLPNKRFYEGCKYIDKIENLTVKLSKKLFKAEHVNVQPTSGVVANLASFFALTKPNDTLMALRVPYGGHISHAEVSAAGIRGLKIRPHPFDNKTMNIDADAMKKDILKTKPKLVLLGGSLFLFPHPVEEAKEAADEVGAKVMYDGAHVLGLIAGERFQDPLKEGADLMVGSTHKTLPGPQGGIIFTSNELKQTIDNAVFPGVVSNHHLHHVAGLGIACAEMLEFGKDYATQTIKNSKKLAECLYDLDFNVLCPDLDFTESHQLVMDIKSVGSSSEISQRLETNNIIVNKNLLPWDEKTETNEPSGIRIGTQEITRRGLKESEMEVVANFINEVVINGKNVKNDAMNFMSDYTTLNYSFKPEEAYEYIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W6</accession>
    <name>F0T6W6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP004962</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644837.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1244"/>
    <dbReference type="EnsemblBacteria" id="ADZ09486">
        <property type="protein sequence ID" value="ADZ09486"/>
        <property type="gene ID" value="Metbo_1244"/>
    </dbReference>
    <dbReference type="GeneID" id="10277695"/>
    <dbReference type="KEGG" id="mel:Metbo_1244"/>
    <dbReference type="eggNOG" id="arCOG04847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682726_0_0_2"/>
    <dbReference type="OMA" id="DIGYRFF"/>
    <dbReference type="OrthoDB" id="89246at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009183">
        <property type="entry name" value="UCP004962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09897">
        <property type="entry name" value="DUF2124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004962">
        <property type="entry name" value="UCP004962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="17743" checksum="F3E80696F1A3D332" modified="2011-05-03" version="1">MEKKTGIVGFTGAFRDNLNGISDGSKIVFTGSAAVCTPFIELLSYAIRDKNHELIFVPNADLKQTKMLKMENNLGYNAVDIKANPSNPDVVVVMGGLAMPKFGCSPDDVLNMVKEISNDHNPKIIGVCFMSIFERSGWINSIPFDVIIDTKMETTIYQHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X0</accession>
    <name>F0T6X0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase like protein, AhpD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644841.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1248"/>
    <dbReference type="EnsemblBacteria" id="ADZ09490">
        <property type="protein sequence ID" value="ADZ09490"/>
        <property type="gene ID" value="Metbo_1248"/>
    </dbReference>
    <dbReference type="GeneID" id="10277699"/>
    <dbReference type="KEGG" id="mel:Metbo_1248"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_4_1_2"/>
    <dbReference type="OMA" id="EEDCCCC"/>
    <dbReference type="OrthoDB" id="120665at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="19"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="99" mass="10573" checksum="284EAECBFE45BF07" modified="2011-05-03" version="1">MKQDKPRSISFTEDVSEELNLAFTNLASEIMKDGALSSKDKSLIALACEVAVKCEHCIKAHKEKALDAGATMDEIKEAAAVAAQVRLGSGLTFASFVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T722</accession>
    <name>F0T722_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646007.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10656">
        <property type="protein sequence ID" value="ADZ10656"/>
        <property type="gene ID" value="Metbo_2443"/>
    </dbReference>
    <dbReference type="GeneID" id="10278915"/>
    <dbReference type="KEGG" id="mel:Metbo_2443"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080151_0_0_2"/>
    <dbReference type="OMA" id="VEGMING"/>
    <dbReference type="OrthoDB" id="88529at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="242" mass="27035" checksum="042A39F3872E7C7A" modified="2011-05-03" version="1">MGRMSIKDIIIDSLKYSASDLKILLLLGIVLVIADLGDELSFLGDISNTVKFTIFVLVVLIAIFESGYVFRIIEDTIKGSSSLPKLNEFRRMFIHGLSEIILIFIYFIVPVALVMVLYVYFFVGDNIDDLSVLSGLILLIIICFAMVILSLFPAVMLHRANNNTDIRSSFDIPAIYHKIRRVGFKRLIIVYSAIILLVAIIRAVIVPDIEGIVPLIIGIISDMLIAPYLLIFTSRVLGLLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T744</accession>
    <name>F0T744_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10678">
        <property type="protein sequence ID" value="ADZ10678"/>
        <property type="gene ID" value="Metbo_2465"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_2465"/>
    <dbReference type="eggNOG" id="arCOG02606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_9_1_2"/>
    <dbReference type="OMA" id="VAYEFEY"/>
    <dbReference type="OrthoDB" id="124641at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="92" mass="10594" checksum="D8DB68CD1D908E63" modified="2011-05-03" version="1">MKMQHVEGQNKGKLVLFALSTCGWCKKTRALIEDLDADYEYVYVDLLKGEEREEAVEMIKKWNPQLSFPTLVINDDVTIIGFKEEEIKEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T769</accession>
    <name>F0T769_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646054.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2490"/>
    <dbReference type="EnsemblBacteria" id="ADZ10703">
        <property type="protein sequence ID" value="ADZ10703"/>
        <property type="gene ID" value="Metbo_2490"/>
    </dbReference>
    <dbReference type="GeneID" id="10278964"/>
    <dbReference type="KEGG" id="mel:Metbo_2490"/>
    <dbReference type="eggNOG" id="arCOG03302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_13_1_2"/>
    <dbReference type="OMA" id="RPRVYYG"/>
    <dbReference type="OrthoDB" id="90656at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="54"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="368" mass="40502" checksum="13A66F61F7F4B9B8" modified="2011-05-03" version="1">MNKNYLILIAAAILIIVSISGYYITAHGSLVGNGNVTITDMANRSVTVPTNVNKVLTTDPTATVMVYMISPDKLLGLNYQTTSEEQKYMPEKYKGLPSVGGWYGAQTGSYEQFVAMEPDLVFEGVTPDTVSQHSSAPSTIEERQQKMGSIPVVGVLDTSNFTSFDPSITFMGKIMGSEDKAAKLVAYNDKVQKEVKDVVYNIPDSEKVTVYYAEDVDGLKTEPPGSVHAQLIDFCGGKNVANVQQNGGNGETDVAMEQVLKWNPEVIITTDPTFFSNVYNNSQWSGVSAVQNKRVYLSPQSPFKWFDRPTGANMIIGIPWVAKILYPDKFQNLNLTSEVKEFYSEFYNYSLTDDEAKNILVGSGLNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T789</accession>
    <name>F0T789_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B</fullName>
            <ecNumber evidence="2">1.2.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646074.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2510"/>
    <dbReference type="EnsemblBacteria" id="ADZ10723">
        <property type="protein sequence ID" value="ADZ10723"/>
        <property type="gene ID" value="Metbo_2510"/>
    </dbReference>
    <dbReference type="GeneID" id="10278984"/>
    <dbReference type="KEGG" id="mel:Metbo_2510"/>
    <dbReference type="eggNOG" id="arCOG01499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="VCIDPHE"/>
    <dbReference type="OrthoDB" id="11387at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="60"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="452" mass="50385" checksum="4A20C2FDEC99F9AF" modified="2011-05-03" version="1">MANYEPPVTDYDSIVENCTCAFCGCNCDDLDYLIKDGHVVGVRHACRLGASKIMEDMDQRLLVPMIRGEDGELAETDWDTALDKAAELIAGAVRPIFYGWSETSIETMHHGLELGELVGAVLDNQATICHGPSLQAVQNAGYPISTLGEVKNRADMCLYTGSNAMNSHPRHMARYSTFPRGYFRPRGRFDRTVVTLDPKYSDTAKMSDIWVGFEQNGDYEFYNAIRAVLKGKKLQKEFISGIPKEDVEELAEAMKNVQFGALFFGLGLTHTLSKQRNIDIAIQMVADLNSYTKWILMPMRGHFNVNGFNIFMAFETGFPYGVDFARGYQRYMNGETNTIDLLTRKECDVFMVIAADPGAHYPGGAVKHLAEIPVIQVDIHWGPSTEIADVVLPGSFVGVEVGGTSYRMDGVPIWMKKAIDKPETCRDDEWIIKEILERVKTIKAEQGALASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T797</accession>
    <name>F0T797_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt transport protein CbiN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0073"/>
    <dbReference type="EnsemblBacteria" id="ADZ08326">
        <property type="protein sequence ID" value="ADZ08326"/>
        <property type="gene ID" value="Metbo_0073"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0073"/>
    <dbReference type="eggNOG" id="arCOG03159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137910_1_0_2"/>
    <dbReference type="OMA" id="SPMPDYA"/>
    <dbReference type="OrthoDB" id="126698at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="PDGLE" evidence="6">
        <location>
            <begin position="14"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="10472" checksum="4861566EE7B4A572" modified="2011-05-03" version="1" precursor="true">MNKKDKTLIIGGVIVCLIIAVLSPFLASPNPDGLEKSAENLAVSDDGLGYQAPFQDYYVAALGDGPLAEVVALAIGILIALGVGFAVAYVLKRRKPKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D6</accession>
    <name>F0T7D6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin (Vitamin B12) biosynthesis CbiG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643716.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0112"/>
    <dbReference type="EnsemblBacteria" id="ADZ08365">
        <property type="protein sequence ID" value="ADZ08365"/>
        <property type="gene ID" value="Metbo_0112"/>
    </dbReference>
    <dbReference type="GeneID" id="10276537"/>
    <dbReference type="KEGG" id="mel:Metbo_0112"/>
    <dbReference type="eggNOG" id="arCOG00651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_0_2"/>
    <dbReference type="OMA" id="GIGCNRG"/>
    <dbReference type="OrthoDB" id="77403at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="SSF159672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiG_N" evidence="2">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG_C" evidence="1">
        <location>
            <begin position="206"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="36456" checksum="EC4AA7C03A5ED22A" modified="2011-05-03" version="1">MKIAIITVTENGKILGESLQNKLLTDNTIIKVQIFHKNVKKTLINSFNKFDCIIGIMATGIMVRNICPLIKTKENDPAVLIIDEKGKHVISLLSGHLGGANKLTHKIADLMGSDPVITTSTDLNSKFGVDCLANKYHFKLIPVSNIKSINSSLINDQKVWIDYNSNYEYLWEDTEVSDSYEHGNNHSQKLLVSNGISDLELVEKKIVLGLGSRKNISTEKVLKAIYLAIANLDIPIERINTLTTGEMKKNEHGILEAAKTINKPLKIISESELKNFTNPDMNDSEFVKSKFGIGGVCEPSSLIEAGTKSRLILRKTSYDGVTVAIALSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D9</accession>
    <name>F0T7D9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase II small subunit</fullName>
            <shortName evidence="12">Pol II</shortName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="12">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">polB</name>
        <name evidence="14" type="ordered locus">Metbo_0115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EC" id="3.1.11.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643719.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0115"/>
    <dbReference type="EnsemblBacteria" id="ADZ08368">
        <property type="protein sequence ID" value="ADZ08368"/>
        <property type="gene ID" value="Metbo_0115"/>
    </dbReference>
    <dbReference type="GeneID" id="10276540"/>
    <dbReference type="KEGG" id="mel:Metbo_0115"/>
    <dbReference type="eggNOG" id="arCOG04455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027850_1_0_2"/>
    <dbReference type="OMA" id="HVQAALI"/>
    <dbReference type="OrthoDB" id="24499at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="PTHR10416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol_E_B" evidence="13">
        <location>
            <begin position="245"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ08368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="501" mass="56653" checksum="A587DF2193FAA1BD" modified="2011-05-03" version="1">MKEDILLKFTNAELLIDDSAYEKIIQQENSVQVADSLIKDISSSNKDFFVLTESVVDNFLQQYLSNYQAPESKLKSDLETNYETQNETVKMPQSKLMDIPLDFHVLKDASKKSYTNGEIKDFTAYFNSRFQKLKAMLTKKPGLRESAPIKEAGNRDGVVNVVGMVNDIRNTKNNHKIMEIEDETGEMTVLIHNENHSLFEQAESIVKDEVIGVIGSKKGTLVIASELIHPSVPRIEEKTMDFSAVFISDIHIGSSTFQEEAFNNFVNWLNGDFGDETQQDIANDVRYLVVAGDVVDGIGIYPHQEEELTIKNIYEQYEEAARLLGQIDNVKMIIAPGNHDACRLAEPQPAIPENYAEDLYKLKNAEFVSNPSLVSLDGFKTLIYHGRSFDDLAMTINGLSHQTSDLIMKELIEKRHLAPIYGERTPLASEYEDHLVIEEIPDIFHTGHVHINAYKKHKGIHMINSGTFQSQTEFQKIYNIMPTCGEVPVLNRGALKVLKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E4</accession>
    <name>F0T7E4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argD</name>
        <name evidence="4" type="ordered locus">Metbo_0120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643724.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0120"/>
    <dbReference type="EnsemblBacteria" id="ADZ08373">
        <property type="protein sequence ID" value="ADZ08373"/>
        <property type="gene ID" value="Metbo_0120"/>
    </dbReference>
    <dbReference type="GeneID" id="10276545"/>
    <dbReference type="KEGG" id="mel:Metbo_0120"/>
    <dbReference type="eggNOG" id="arCOG00914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_2"/>
    <dbReference type="OMA" id="MVPNYNP"/>
    <dbReference type="OrthoDB" id="20774at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="217"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="390" mass="42119" checksum="436DDDB6832DD0C1" modified="2011-05-03" version="1">MNTQEIIEMDSKYVMQTYGRHKIALVKGKDTTVWDADGNSYIDCVAGIAVNNVGHSNPKVGEAICNQVKKLIHTSNLYYTEEQVTLAKLLVDVSPHQKAFFCNSGAEANEGAIKLARKHSGKGEIITMINSFHGRTITTITATGQPKYQKGFEPLTPGFKYAPYGDIEAVKEMISPSTAAVLVEPVQGESGVIVPPEGYLKQLKEVCEDSNVLLIFDEVQTGFGRTGEMFASQTFGVTPDITSLAKAIAGGFPMGAVLANNDVAESFVPGDHAATFGGNPLGCAAAKASINFILEEKLLDKSKENGSYFVEKLLKLQNQHELINEVRGKGLMVGVEMKTDCGCMVDELFKEGIIVNCAAGNVLRFVPPLTITKEQIDTVTAALNNVLSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G0</accession>
    <name>F0T7G0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0136"/>
    <dbReference type="EnsemblBacteria" id="ADZ08389">
        <property type="protein sequence ID" value="ADZ08389"/>
        <property type="gene ID" value="Metbo_0136"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0136"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136066_0_0_2"/>
    <dbReference type="OMA" id="ECYGTGH"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="191"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="28293" checksum="1BFFC01347256742" modified="2011-05-03" version="1">MMTNQDAGTLNQIKIFGTKNGLNIVNSSIKSKILEILEENERSGAEIVKLTGKSKATISAHLNDLVGMGIIESKPNPKDGRSKIFFIKSSYLGDLTRKNDFNKELDDYITEGVVNSEDPFAFFRFVFRTFRVALIEEGVNIDPILHNAGIKVGETFYSKLKDSQTDRFIENIADFWQKNKLGKIKTETLEPLTLKAYDCFECEDLPKIGRSACAFDSGILEAIFSAHFGRKVDVDEIKCFAKGDDYCSFVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J0</accession>
    <name>F0T7J0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644909.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1316"/>
    <dbReference type="EnsemblBacteria" id="ADZ09558">
        <property type="protein sequence ID" value="ADZ09558"/>
        <property type="gene ID" value="Metbo_1316"/>
    </dbReference>
    <dbReference type="GeneID" id="10277767"/>
    <dbReference type="KEGG" id="mel:Metbo_1316"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_1_2"/>
    <dbReference type="OMA" id="AIVFPSH"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="178"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="372" mass="41857" checksum="1B23821C967ACB61" modified="2011-05-03" version="1">MRIVQTPVRFYPFIGGVENYVYYLSKELVNLGHNVKVVCANEPKVEAEQNVDGVEVSRLNYLFKVANTNITPDLPLKLRGEEFDIIHTHIPTPWSADWSRIIANRKNKPLVVTYHNDIIGTGFANHVANFYNKTALKKLLNRADKIIITQPNYLNYSPYLSSYEKKVEVIPNGVDVDKFKPLNIEKKQNTLFFLSLLDEFHGYKGLDYLLEALQIVKKSIADVKLIVGGKGVLMDHYIQKAESMGLKANVEFHGFIPDESMAMYYSQANLFVLPSISSLQEGFGIVALEALACETPVITTDIVGVSADLKEKNAGLSVAPKNSQVLAEAIIKLLDDHELRTKMGVNGRNLVKASYTWKGIARKMEKVYYELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K2</accession>
    <name>F0T7K2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1329"/>
    <dbReference type="EnsemblBacteria" id="ADZ09570">
        <property type="protein sequence ID" value="ADZ09570"/>
        <property type="gene ID" value="Metbo_1329"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1329"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="70"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="205"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="394" mass="45117" checksum="1515544E6AB928B3" modified="2011-05-03" version="1">MIRIKNKKNVEKLRFKAEELLNQKINELEIDDSKMPENINELIHELQIHHIELEMQNEELRKSQIELQTSNIKYFELYNFAPCSYFTIDTDGMIIDVNFAGTSLMGIDKIYLINRAFIQFVAHDSRNKFIKLIENVTSYKEIIKCELELLKEKKPFPVIMEINLNSNSEEKCPSFMITVVDISDLKNAEIKVRKSLNEKKVLLREINHRVKNNLQIISSLLHLQEGCAESEDAMDVLKESQGRVKSMAMVQEKLSQSPNYSNINLKDYVEKLVHDILYSYGSLETIKTDLNIEDLNLNMDTSILLGLIINELVTNIVKYAFKGTEGTISVKIKSKSELIDIIIADNGIGISENIDLENSDTLGLQLVTNLVEQLNGNINLNTSKGTKYNISIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M6</accession>
    <name>F0T7M6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1354</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644945.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1354"/>
    <dbReference type="EnsemblBacteria" id="ADZ09594">
        <property type="protein sequence ID" value="ADZ09594"/>
        <property type="gene ID" value="Metbo_1354"/>
    </dbReference>
    <dbReference type="GeneID" id="24964553"/>
    <dbReference type="KEGG" id="mel:Metbo_1354"/>
    <dbReference type="eggNOG" id="arCOG02392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926262_0_0_2"/>
    <dbReference type="OMA" id="CTIAAIN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="238"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="34205" checksum="9AECF1DE709D7072" modified="2011-05-03" version="1">MVVEDDGILATALQVKLENWGYKSPVAFTSQDAVKQFHKTKPDLVIMDITLKGKLNGIETAELIDSELQTPIIYYSSSTDKELSARLKNIQNRDYVSKTSEDETLRSSIEKHIKLKSNRDFEKLMTQKTSRKDLTDFNKEEGDVNTNRNLNTLKNNVSYPDTESSKPKNVEAMNDKCAEANSVSSVGTFNNVSNFDESIEAKNYGSHQTIETELKKLETNFTKIFKDSKSQHQKIVELNNLVEEYKGLLAEKDIQINELKAEQTKLEERLIESKDQFRTVLNEMCSVNDQINKFIGTLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W0</accession>
    <name>F0T7W0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="4">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="4">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pus10</name>
        <name evidence="6" type="ordered locus">Metbo_0195</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643798.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0195"/>
    <dbReference type="EnsemblBacteria" id="ADZ08447">
        <property type="protein sequence ID" value="ADZ08447"/>
        <property type="gene ID" value="Metbo_0195"/>
    </dbReference>
    <dbReference type="GeneID" id="10276621"/>
    <dbReference type="KEGG" id="mel:Metbo_0195"/>
    <dbReference type="eggNOG" id="arCOG01015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028780_2_0_2"/>
    <dbReference type="OMA" id="DGGLYIK"/>
    <dbReference type="OrthoDB" id="61936at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="PTHR21568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="5">
        <location>
            <begin position="59"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="407" mass="46803" checksum="6AC3C14839ACB734" modified="2011-05-03" version="1">METLIHDKLISAMDLTQGNICNHCLGRIFSKEMEGPGNVIRGERLKQSLEDLDETYSKEDKCWICEDLFDNMDNMMQQALKTVEDEEINFSNFLVGCRVDPDVLEREEYLHQKLGANVENIKKEINREFGKKLASILSKEVEFDSPNLVFMADFKSEKVKITINPIFIESKYRKLVRDIPQTKWPCNRCKGRGCEKCNFTGKMYPVTVEELISDVMIKAAKGTGAKFHGAGREDIDVRMLGSGRTFVLEILEPKIRELDLETLQEEVNNYAESKVEISEMKYVPRNRKAEIKQSSTDTYKVYKALVEVDDEIDPTALDSLKSLDIINQRTPIRVSHRRADKIRTRKIRKLDYNWLEPKIFEITLECEGGLYIKELISGDENRSKPSVAEVLGTNARCVQLDVLEVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W1</accession>
    <name>F0T7W1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="8">SRP54</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">srp54</name>
        <name evidence="10" type="ordered locus">Metbo_0196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0196"/>
    <dbReference type="EnsemblBacteria" id="ADZ08448">
        <property type="protein sequence ID" value="ADZ08448"/>
        <property type="gene ID" value="Metbo_0196"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0196"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="9">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="447" mass="49403" checksum="6E34A735DC933AC8" modified="2011-05-03" version="1">MVRIMLGNLGKNLTKTMKKLAGMAIIDEEVVKEVIKDIQRALIQSDVNIKLVLNLSKTIEDRSLNEEPPKGVTPKEHVVRIVYEELVNLLGSSAQEVEIDKRPYKILFMGLQGSGKTTSIGKLTKYLQKKGFNPAIVSTDTWRPAAYEQLLQLTEPMNVPVYGDHENKDALDLARTGLKAAKKNDVIIVDTAGRHKEESDLLAEMESLSTIVDPDEVIMVIDGTIGQQAGEQALAFNNATKIGSIIITKLDGSAKGGGALSAVAEIGAPIKFIGTGERVEDFEAFDPDRFISRLLGMGDIRTLLERAEEIAEEEGDMGSVDAMLSGKFTLKDMYSQFEMMNKMGPMQQVMNMIPGAGGKLPKNASQVTEEKLSKYKILMDSMTDQELEHPEVIKQSRVKRIARGSGMKNEDVKELLKYYNVTKKAMKGFGKRKMGGPLGQMMKQMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y0</accession>
    <name>F0T7Y0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643818.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08467">
        <property type="protein sequence ID" value="ADZ08467"/>
        <property type="gene ID" value="Metbo_0215"/>
    </dbReference>
    <dbReference type="GeneID" id="10276641"/>
    <dbReference type="KEGG" id="mel:Metbo_0215"/>
    <dbReference type="eggNOG" id="arCOG11271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922809_0_0_2"/>
    <dbReference type="OMA" id="AMYIAVE"/>
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        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
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    </feature>
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        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="131" mass="13780" checksum="E9B3880A6914AA05" modified="2011-05-03" version="1">MVKEMIKRKPLIIGLITVIAFYVVTNILSTLDATITTFLAIGILVGFMVGENYLAGAINGALFGVIGALIVALILVVTYTLYGYGAYLGLVATQLIISCVLYIIVAVVGGVIGAQVRIETDSKDVAPEVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T814</accession>
    <name>F0T814_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09640">
        <property type="protein sequence ID" value="ADZ09640"/>
        <property type="gene ID" value="Metbo_1400"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1400"/>
    <dbReference type="eggNOG" id="arCOG11306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998756_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="14390" checksum="953B72BC2F8FA9A8" modified="2011-05-03" version="1">MVVVGLQAAPAVSAAHVSDKQVTDKFMVKYPGGGQSGDAATYKVYKYALNHFYIHTYYYNYNKKTKKYYRNGASDWMDLKKITANKIRILSPPMEGKPYVDIVYTKHSVTWYYWNDLRKQLKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T844</accession>
    <name>F0T844_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09670">
        <property type="protein sequence ID" value="ADZ09670"/>
        <property type="gene ID" value="Metbo_1432"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1432"/>
    <dbReference type="eggNOG" id="arCOG06478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OMA" id="FIFAINA"/>
    <dbReference type="OrthoDB" id="129151at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="17914" checksum="8E495809929DBE6C" modified="2011-05-03" version="1" precursor="true">MKHNNVLFGILAILLGLAVIAFPLISVFIVSDIVAIGLIFIGIWLLAQSVVTWSESKAMSILALIIGIIGVIVGIGLFGKVLAFSIFAGIIIYLGGIFLIVSGVISLISGKGNAGRAGGFLGIVLGILYLIIGVYALNPFYLALLIGLWLVLTGIFLVLEPNPEVPAPKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T854</accession>
    <name>F0T854_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645031.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1442"/>
    <dbReference type="EnsemblBacteria" id="ADZ09680">
        <property type="protein sequence ID" value="ADZ09680"/>
        <property type="gene ID" value="Metbo_1442"/>
    </dbReference>
    <dbReference type="GeneID" id="10277893"/>
    <dbReference type="KEGG" id="mel:Metbo_1442"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088479_0_0_2"/>
    <dbReference type="OMA" id="KPTIFLM"/>
    <dbReference type="OrthoDB" id="72889at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30197" checksum="F12793E7DA4D85AF" modified="2011-05-03" version="1">MKKINALGIKYPNSKILVENVLKQNMEDEIIINLKKSAEQGLEELSEKYGYNFVIETNGNELVVRMTQKDIEIEKLDVTGQTCPGPVIIVGDKLASMQVGQRVEIDSNMKETLDDLVISAPEMNGVVVEEGETNGNYYVIIEKVETKTVSQGIKRNKVLVVQSNGIGNAERAYATFIFSKAALSMGKEVTIFLLMDGVSIARKGNAEKVKHPAFERLDKLMKPIIEQGAQIYVCELSAEFRGIKQTDLVDGTKLAGAATYITLLSDPSFAVVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T857</accession>
    <name>F0T857_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645034.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1445"/>
    <dbReference type="EnsemblBacteria" id="ADZ09683">
        <property type="protein sequence ID" value="ADZ09683"/>
        <property type="gene ID" value="Metbo_1445"/>
    </dbReference>
    <dbReference type="GeneID" id="25394732"/>
    <dbReference type="KEGG" id="mel:Metbo_1445"/>
    <dbReference type="eggNOG" id="arCOG02356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_282645_0_0_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="139"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="212"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="265"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="337"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="390"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="462"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="578"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="650"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="887"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="505"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1104" mass="126409" checksum="1057A0BEA3D52A93" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T861</accession>
    <name>F0T861_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF362 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645038.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1450"/>
    <dbReference type="EnsemblBacteria" id="ADZ09687">
        <property type="protein sequence ID" value="ADZ09687"/>
        <property type="gene ID" value="Metbo_1450"/>
    </dbReference>
    <dbReference type="GeneID" id="10277901"/>
    <dbReference type="KEGG" id="mel:Metbo_1450"/>
    <dbReference type="eggNOG" id="arCOG02446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762102_0_0_2"/>
    <dbReference type="OMA" id="FHANGPF"/>
    <dbReference type="OrthoDB" id="64454at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007160">
        <property type="entry name" value="DUF362"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04015">
        <property type="entry name" value="DUF362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF362" evidence="1">
        <location>
            <begin position="42"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="363" mass="40472" checksum="8E710715DCA77631" modified="2011-05-03" version="1">MDDRTVFVEGVSSDSDSLYKSIEEVLLKSTNNLDWLCKDDLVLLKPALNSPDPYPSTTHPMAVQVVREILSEKGAEVVVGDQSGLKDVLQDQSGVVHGKTHDNYIKSGMGTSEGRFIGFESEGWDEGYIHHQSKYTRSWPKGFNITKWIEEVDHIINLPRLSTHSQAGVTLGFKNMVGILRDDSRMDFHANGPFNNFIKTEAHGSKLHSIDDGSHSFIEKIVEIHDAIREKLRTTLFVATKAQTTFGPNTNALKIGKLEIGTAHVVKLKPGLIFASNDPVSAESFALAVLKDLRKSLPSLPKFSSQILLFSNSNVKQLDETPINDMTFIYHAIKIGLGEMPNQIVYKNVPDNLKERLNNYIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T872</accession>
    <name>F0T872_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyltransferase</fullName>
            <ecNumber evidence="8">6.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_0232</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643835.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0232"/>
    <dbReference type="EnsemblBacteria" id="ADZ08484">
        <property type="protein sequence ID" value="ADZ08484"/>
        <property type="gene ID" value="Metbo_0232"/>
    </dbReference>
    <dbReference type="GeneID" id="10276662"/>
    <dbReference type="KEGG" id="mel:Metbo_0232"/>
    <dbReference type="eggNOG" id="arCOG01187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009164_0_0_2"/>
    <dbReference type="OMA" id="VQHHYAH"/>
    <dbReference type="OrthoDB" id="6307at2157"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046944">
        <property type="term" value="P:protein carbamoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
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        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="10">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="11">
        <location>
            <begin position="199"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ08484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="768" mass="85356" checksum="ADCFCC2F1ED66DA3" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A8</accession>
    <name>F0T8A8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na+/H+ antiporter MnhB subunit-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0268</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0268"/>
    <dbReference type="EnsemblBacteria" id="ADZ08520">
        <property type="protein sequence ID" value="ADZ08520"/>
        <property type="gene ID" value="Metbo_0268"/>
    </dbReference>
    <dbReference type="GeneID" id="10276700"/>
    <dbReference type="KEGG" id="mel:Metbo_0268"/>
    <dbReference type="eggNOG" id="arCOG03079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101659_3_2_2"/>
    <dbReference type="OMA" id="INPGYLN"/>
    <dbReference type="OrthoDB" id="119875at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="MnhB"/>
    </dbReference>
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        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="157" mass="16828" checksum="03E02FA78E868A84" modified="2011-05-03" version="1">MSTILKLFAFPASVVFMCYGALTILGGHITPGGGFQGGAMIAAGIIFCLVVYGVDKTPIKLSHNFMASLESIGAIGYIFLGLAGLFTTGFFLYNLGVDLYHIVPPYIVNIFDYPDPIHAGIIPYLNFVVGLKVLVGLSAVVITFLESDKLIKKMESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I1</accession>
    <name>F0T8I1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine active site containing 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645083.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09732">
        <property type="protein sequence ID" value="ADZ09732"/>
        <property type="gene ID" value="Metbo_1500"/>
    </dbReference>
    <dbReference type="GeneID" id="10277951"/>
    <dbReference type="KEGG" id="mel:Metbo_1500"/>
    <dbReference type="HOGENOM" id="CLU_054347_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="367" mass="43172" checksum="3A70CB1D3ADEF772" modified="2011-05-03" version="1">MEGFTDFKERLTNNRSKTAIYVWFGSIIFAFLFTVLIWFLGPNLNHFTVTLLPLNNINHYYWKLPDRNFWTMVIVWSFYIANQLLIWGAIYWAQKNLTKEKTNPTYDLTKYNVAVLSITVFFIFLHLIQTHIWFDGLAQDVPIITSQGSVILMLAIALVLLNPVQGLFLGKKAGKPFTARVTEFIRHTHMYIISWALIYTFWFHPMATDPQLLTGFFFMFLLFTQMALAYTKVHLDLRWIVILQSWVAIHALFVAFYNTLFFGNTDMWPMFFSGFAFMFVFTYMYALNVKRNVKFLVTAAYILLVAFIYIPAPYGFGRDPSFLLRLEFLWIPIILYGLAALIALIIFIWLKLRNKPRSIEKLKETPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M2</accession>
    <name>F0T8M2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643915.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0312"/>
    <dbReference type="EnsemblBacteria" id="ADZ08564">
        <property type="protein sequence ID" value="ADZ08564"/>
        <property type="gene ID" value="Metbo_0312"/>
    </dbReference>
    <dbReference type="GeneID" id="10276744"/>
    <dbReference type="KEGG" id="mel:Metbo_0312"/>
    <dbReference type="eggNOG" id="arCOG10258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285074_0_0_2"/>
    <dbReference type="OMA" id="MIRIERT"/>
    <dbReference type="OrthoDB" id="118960at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11859" checksum="A782923496729711" modified="2011-05-03" version="1">MIIMERTCGSYRCDVFQDGKLIGKMDGVNLIQWFVKNSYRYTGTFSRFTTMDPNRNQSNITVDIVFANKRIIIKDARVEWIKNQNQNGTFQATKIESYNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P4</accession>
    <name>F0T8P4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trm1</name>
        <name evidence="9" type="ordered locus">Metbo_0334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643937.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0334"/>
    <dbReference type="EnsemblBacteria" id="ADZ08586">
        <property type="protein sequence ID" value="ADZ08586"/>
        <property type="gene ID" value="Metbo_0334"/>
    </dbReference>
    <dbReference type="GeneID" id="10276771"/>
    <dbReference type="KEGG" id="mel:Metbo_0334"/>
    <dbReference type="eggNOG" id="arCOG01219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="OMA" id="VFYNPVM"/>
    <dbReference type="OrthoDB" id="70865at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="PTHR10631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 8" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="386" mass="42621" checksum="C2E86437426C2C6A" modified="2011-05-03" version="1">MESKVINEGKVSIKVPEYEKISSKAPVFYNPVMELNRDLSVAALTVFSETVDHNITVCDTFGGTGIRGIRYVKEIEGVEKAVVNDLNPVAVEFAKENVNLNGLKGVDVRNEDANIILRKCKGQFDVVDLDPFGTPSYFVDSAAHSIRSGGMICVTATDTSALCGTYTEPCIRKYSAVPLKTEYCHENGLRILAGFVARTFAKYKKFIEIKFSHSTEHYMRIYATVGKGGENTDNSLKALGYIAHCRDCMNRMVIKGLAPIISDKCPICGGDILIGGPLWCGDMYDPEFVSSMIENFKKLELNTEPAAIKLLETCLSESRAPVSFYEIHKVCKNLKIGAPPLQKIMDQLKDGGYFVSKTHFSPTSLKTDAGIIELKKIILNLKSDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P8</accession>
    <name>F0T8P8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP_bind_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643941.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0338"/>
    <dbReference type="EnsemblBacteria" id="ADZ08590">
        <property type="protein sequence ID" value="ADZ08590"/>
        <property type="gene ID" value="Metbo_0338"/>
    </dbReference>
    <dbReference type="GeneID" id="10276775"/>
    <dbReference type="KEGG" id="mel:Metbo_0338"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OMA" id="IGDEACP"/>
    <dbReference type="OrthoDB" id="57500at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="32"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="31653" checksum="E8B6AE187C997CAD" modified="2011-05-03" version="1">MKIDGDAFNQRIIAGAREVIDEYKLIDPNELVVVALSGGKDSVLTLHILADLMGELEFDLVAVSIDEGVAGYRSEGVEAARLNCESLGIKLVEGSFKDEFGFKLDDLHGMYQSSCIPCGVFRRQLLNKISHQLGADKLATGHNLDDEIQSFLMSLAKAEIRRFYKFGPKLDKIHPDMVPRIKPLWKTHEKDVGIWAVLNNIEVHFAECPYSATSLRSKIKGYLNQLEEKRPGTKLSMLKSFSKSFKFEKKDVQMGVCSLCGEPSSLDVCMACEMMAEINDRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X6</accession>
    <name>F0T8X6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645155.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1577"/>
    <dbReference type="EnsemblBacteria" id="ADZ09804">
        <property type="protein sequence ID" value="ADZ09804"/>
        <property type="gene ID" value="Metbo_1577"/>
    </dbReference>
    <dbReference type="GeneID" id="10278033"/>
    <dbReference type="KEGG" id="mel:Metbo_1577"/>
    <dbReference type="HOGENOM" id="CLU_1987637_0_0_2"/>
    <dbReference type="OrthoDB" id="120126at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="14663" checksum="02DEEAB25FF4B86E" modified="2011-05-03" version="1">MRVSEGVTRGKIAELNNPEIFREREEVVVFKRDEFNRIYRSMMEQINHVNQIHLYLDESETWKLMGYWPKIMERVYIIDRNMDALLKNEPLQCYLDAFLESSVGSFSGEVAASKGKVSVKDMLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z7</accession>
    <name>F0T8Z7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645176.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1598"/>
    <dbReference type="EnsemblBacteria" id="ADZ09825">
        <property type="protein sequence ID" value="ADZ09825"/>
        <property type="gene ID" value="Metbo_1598"/>
    </dbReference>
    <dbReference type="GeneID" id="10278055"/>
    <dbReference type="KEGG" id="mel:Metbo_1598"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_4_0_2"/>
    <dbReference type="OMA" id="TMMTNVV"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="PTHR43078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="7">
        <location>
            <begin position="5"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35774" checksum="BFBB39382442E3DB" modified="2011-05-03" version="1">MLRVLITGVAGFIGSHLATKFIEEGCYVIGIDNFLTGSPENISDIIDHENFEFIEHDIINPIKIENNIDIVLHFACPASPEDYMEYQLETLRVDSFGTHNTLEIAKEKNSRYIFASTSEIYGDPLINPQPESYWGNVNTHGVRSVYDESKRFSEALSMAYFRKNKVDIRIVRIFNTYGPRMKINDGRVVPNFIAQALTHKDLTVYGDGKQTRSFCYVDDLVDGIYKISIKDGLDGEIMNLGNPDEYKIIDFAEIILQKINSNSKIVYQELPEDDPKLRCPDITKVKGLIDWKPTVSLDEGLDNTIKFLKTKLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T925</accession>
    <name>F0T925_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxaloacetate decarboxylase alpha subunit</fullName>
            <ecNumber evidence="4">6.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643998.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0395"/>
    <dbReference type="EnsemblBacteria" id="ADZ08647">
        <property type="protein sequence ID" value="ADZ08647"/>
        <property type="gene ID" value="Metbo_0395"/>
    </dbReference>
    <dbReference type="GeneID" id="10276832"/>
    <dbReference type="KEGG" id="mel:Metbo_0395"/>
    <dbReference type="eggNOG" id="arCOG02095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_4_2_2"/>
    <dbReference type="OMA" id="GYHALEM"/>
    <dbReference type="OrthoDB" id="19729at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005776">
        <property type="entry name" value="OadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01108">
        <property type="entry name" value="oadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="499"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="570" mass="63104" checksum="C96CFCCC666AD183" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T927</accession>
    <name>F0T927_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644000.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0397"/>
    <dbReference type="EnsemblBacteria" id="ADZ08649">
        <property type="protein sequence ID" value="ADZ08649"/>
        <property type="gene ID" value="Metbo_0397"/>
    </dbReference>
    <dbReference type="GeneID" id="10276834"/>
    <dbReference type="KEGG" id="mel:Metbo_0397"/>
    <dbReference type="eggNOG" id="arCOG04213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="OMA" id="GTKEWAD"/>
    <dbReference type="OrthoDB" id="46491at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="199"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="367" mass="40723" checksum="2767F09994AC9B27" modified="2011-05-03" version="1">MEKIKVAIVGIGNCASSLIQGIHYYEDKNPDEAIGLMHWDIGGYKPSDIEVVAAFDIDCRKVGKDVSEAIFAKPNCTQVFCSDIPEYGVEVSMGKILDGVAPHMADHKKEFAFVVSDAKENEKDEVVKIIKDSGAEVLLNYLPVGSEEATRFYAQCALDAGVAFINNMPVFIVSNPEWAAKFEAKGIPIVGDDIKAQIGATITHRTLANLFRDRGVKLERTYQLNTGGNTDFLNMLNRSRLDSKKESKTEAVQSVLDERLEDENIHIGPSDYVTWQKDNKLCFLRMEGKTFGDVPMNIELRLSVEDSPNSAGCVIDAIRCCKLALERGVGGQLKSISAYTMKHPPEQFTDDLAKEMVDEFIEGERER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T987</accession>
    <name>F0T987_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645189.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09838">
        <property type="protein sequence ID" value="ADZ09838"/>
        <property type="gene ID" value="Metbo_1611"/>
    </dbReference>
    <dbReference type="GeneID" id="10278068"/>
    <dbReference type="KEGG" id="mel:Metbo_1611"/>
    <dbReference type="HOGENOM" id="CLU_838408_0_0_2"/>
    <dbReference type="OrthoDB" id="93239at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="38731" checksum="70E46E22A8DA87BC" modified="2011-05-03" version="1">MDRDTLIKHKLMMQSDRINKYLMNRINGLYSLILIALILNIFIYFEFNSINFLFNVENRLELMVFILTVILVDATLALLSFTYLLVIKEDDQNSMDLKGTTILSDKSAGEISYIIKNWKEGLKDKSRHLQSQKKKLLEYIDKIGIKDGNLDSEPRNKHGKHEKRSDVFKLREIDRDSRMPTYVIESKLIQISEDLDVFDDDLEELERNDEDHELKSSLIEARDLYEKLKDLENEILENLTLLEYSMEIYNFKTLKIIGKLFFQSSISIIIGFFILGILVFALQNDVILAVLQSFGSLPLQLIIFFASIFVIDGSWSFLRAVYSFFKLYIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A5</accession>
    <name>F0T9A5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-oxalocrotonate tautomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645207.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1630"/>
    <dbReference type="EnsemblBacteria" id="ADZ09856">
        <property type="protein sequence ID" value="ADZ09856"/>
        <property type="gene ID" value="Metbo_1630"/>
    </dbReference>
    <dbReference type="GeneID" id="10278087"/>
    <dbReference type="KEGG" id="mel:Metbo_1630"/>
    <dbReference type="eggNOG" id="arCOG02240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_5_2_2"/>
    <dbReference type="OrthoDB" id="118012at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7600" checksum="AE67A3736F240944" modified="2011-05-03" version="1">MPMVQINVWKGFEEEKIEYLIKNITQAFVEVDVPAQAVEILVNEVPKSHWGVGGELCSVKFKDVGPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D2</accession>
    <name>F0T9D2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645234.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1657"/>
    <dbReference type="EnsemblBacteria" id="ADZ09883">
        <property type="protein sequence ID" value="ADZ09883"/>
        <property type="gene ID" value="Metbo_1657"/>
    </dbReference>
    <dbReference type="GeneID" id="10278114"/>
    <dbReference type="KEGG" id="mel:Metbo_1657"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101012_1_0_2"/>
    <dbReference type="OMA" id="PDYGIRA"/>
    <dbReference type="OrthoDB" id="21535at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="27219" checksum="14FAE90E261813F6" modified="2011-05-03" version="1">MDLKKILGLQKPDIKKMEREGDIKGLTRLLKFEQDESVRREAAFAIGKITGPNSGMESTDENPKPTKQSVEELVKSLKSDDHELQKQATVQLVEIGSPSVKPLLKSLEDKNWKIRWYASEILGKIGDERAVPGLIETLGDENSGVRNKSMVALVEIGEPSVDLLTSALSNDKWQIRSQAAEALGVIGLKKSVEPLIQTLKDGNSWVRKAAAESLGSIGDKKAVSPLKNLLKDDSLEVQEAVSNALEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J7</accession>
    <name>F0T9J7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ThiamineS protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644096.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0493"/>
    <dbReference type="EnsemblBacteria" id="ADZ08745">
        <property type="protein sequence ID" value="ADZ08745"/>
        <property type="gene ID" value="Metbo_0493"/>
    </dbReference>
    <dbReference type="GeneID" id="10276933"/>
    <dbReference type="KEGG" id="mel:Metbo_0493"/>
    <dbReference type="eggNOG" id="arCOG00535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_9_4_2"/>
    <dbReference type="OMA" id="MEVKIVI"/>
    <dbReference type="OrthoDB" id="122856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="66" mass="7269" checksum="0DFD4618D4DB2E3E" modified="2011-05-03" version="1">MKINVKVGNNEDTVTISGEKTIKDLLESMEIAAETVVVKKNDYIVIDEEILEDGDSIEVIQVIYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L2</accession>
    <name>F0T9L2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl coenzyme M reductase system, component A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0508</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644111.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0508"/>
    <dbReference type="EnsemblBacteria" id="ADZ08760">
        <property type="protein sequence ID" value="ADZ08760"/>
        <property type="gene ID" value="Metbo_0508"/>
    </dbReference>
    <dbReference type="GeneID" id="10276948"/>
    <dbReference type="KEGG" id="mel:Metbo_0508"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="LPGGRMH"/>
    <dbReference type="OrthoDB" id="25719at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="280"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="317"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="530" mass="59113" checksum="8383DF1F3417F936" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L4</accession>
    <name>F0T9L4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="4">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mer</name>
        <name evidence="6" type="ordered locus">Metbo_0510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mer family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644113.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0510"/>
    <dbReference type="EnsemblBacteria" id="ADZ08762">
        <property type="protein sequence ID" value="ADZ08762"/>
        <property type="gene ID" value="Metbo_0510"/>
    </dbReference>
    <dbReference type="GeneID" id="10276950"/>
    <dbReference type="KEGG" id="mel:Metbo_0510"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_2"/>
    <dbReference type="OMA" id="VCWAAEA"/>
    <dbReference type="OrthoDB" id="46136at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03555">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="321" mass="33351" checksum="6F845102DE1C3F92" modified="2011-05-03" version="1">MKFGIEFVPNEPIDKIVKLVKLAEDVGFEYAWITDHYNNKNVYETLALIAEGTETIKMGPGVTNPYVRSPAITAAAVATLDELSNGRATLGIGPGDKATFDALGIEWTKPVSTIKDAITMMSTLMAGEKTESGAQLGGVKAVQEKVPIYMGAQGPMMLKTAGGFSDGALINASNPKDFEAAVPLIKQGAEEAGKSISDVDVAAYTCCSIDDDAGKALGAAKIVVAFIAAGSPPPVFERHGLPTDTGAKFGGMLAKGDFGGAIGAVDDALMEAFSVVGTPKEFVPKIEALAEMGVTQYVAGSPIGPNKEKSIKLLGEVIDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P0</accession>
    <name>F0T9P0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645270.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09919">
        <property type="protein sequence ID" value="ADZ09919"/>
        <property type="gene ID" value="Metbo_1694"/>
    </dbReference>
    <dbReference type="GeneID" id="10278151"/>
    <dbReference type="KEGG" id="mel:Metbo_1694"/>
    <dbReference type="HOGENOM" id="CLU_161125_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13680" checksum="594572B4F2A022D1" modified="2011-05-03" version="1">MNVPVIFLIIYVLCICSLAIIGYYDNPNETLIYEIVEYNTPKITASPNGSSVFITGILRDNSEHTFSNVMVNVAGIGEHGQVVNDKNIEIPHMGNYSNVEYNVSLENDSTVVAGQIQVLNATIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R5</accession>
    <name>F0T9R5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645295.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1720"/>
    <dbReference type="EnsemblBacteria" id="ADZ09944">
        <property type="protein sequence ID" value="ADZ09944"/>
        <property type="gene ID" value="Metbo_1720"/>
    </dbReference>
    <dbReference type="GeneID" id="10278177"/>
    <dbReference type="KEGG" id="mel:Metbo_1720"/>
    <dbReference type="eggNOG" id="arCOG01962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_1_2"/>
    <dbReference type="OMA" id="QIGMFVL"/>
    <dbReference type="OrthoDB" id="47913at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="12"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="397" mass="43037" checksum="0EC637CE2E1CCBB4" modified="2011-05-03" version="1">MISEQFILFGSVLLFICIVISKTSHRLGIPSLLFFLLIGMLAGSEGIGGIYFDNPSITQFIGIIALVIILFSGGLDTKFSEIKPILGQGLILATVGVFITAILTGIFLYWFLHIPLLESLLIGSIISSTDAAAIFAIFNSKKMGLKNNIAPLLELESGTNDPMAYFLVTTLIFLIINPTTSLTAMVILLIKSLGLGILVGFFFGKGSVWIINNIKLHTEGLYSVFTLAIAFLTFSVSYFIGGNGFLSVYIAALILGNSHFVHKTEQIQFFDGIALLMQIIMFLTLGLLVFPSQIVPVLGIGIFVSVVLILVARPVAVFLCLIPFKVGFKDKIFISWVGIKGAVPIIFATYPIVAGIAGSEIIFNIIFFITIISALVQGSTIEILSKYLGLYIPNKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S1</accession>
    <name>F0T9S1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SNARE associated Golgi protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1726"/>
    <dbReference type="EnsemblBacteria" id="ADZ09950">
        <property type="protein sequence ID" value="ADZ09950"/>
        <property type="gene ID" value="Metbo_1726"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1726"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_2_2"/>
    <dbReference type="OMA" id="CGIAKMN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="25158" checksum="2884A5D1D1B02635" modified="2011-05-03" version="1">MISVVELVSNFAIHIIQILGYWGVFIGMTLESACIPLPSEIIMTFSGYVVWQGTTNMTLLGITLVGAVGNLIGSLIAYFVGLKGGRPFLEKYGKYIFISHSKLELADRWFERYGWEAVLISRMLPVIRTFISLPAGIANMDLKKFSLYTFLGSLPWSFALAYIGVQLGPNWDVIESYFHIMDIGVFIGIVGLIIYLVLSYRSKSKTTGSEPEKQNKNRKIRDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U0</accession>
    <name>F0T9U0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645320.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1746"/>
    <dbReference type="EnsemblBacteria" id="ADZ09969">
        <property type="protein sequence ID" value="ADZ09969"/>
        <property type="gene ID" value="Metbo_1746"/>
    </dbReference>
    <dbReference type="GeneID" id="10278203"/>
    <dbReference type="KEGG" id="mel:Metbo_1746"/>
    <dbReference type="HOGENOM" id="CLU_133846_1_0_2"/>
    <dbReference type="OrthoDB" id="136598at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045990">
        <property type="entry name" value="DUF5946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19371">
        <property type="entry name" value="DUF5946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="16789" checksum="4C22B7A25A7F6331" modified="2011-05-03" version="1">MSENYKSKPEMFHELSCYSSSHGDPEFIHQYVVDAFALETADDETKNIRVAFALIGLYLHVEKNFTGKEVQNAHIKLGKQRKTWPRFVLPLNRGDIGIKNVMECPEGFKRDMAIENWCLSVWNAYSSCHEAVRELVQHELWRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V4</accession>
    <name>F0T9V4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644128.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0525"/>
    <dbReference type="EnsemblBacteria" id="ADZ08777">
        <property type="protein sequence ID" value="ADZ08777"/>
        <property type="gene ID" value="Metbo_0525"/>
    </dbReference>
    <dbReference type="GeneID" id="10276970"/>
    <dbReference type="KEGG" id="mel:Metbo_0525"/>
    <dbReference type="eggNOG" id="arCOG07795">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197246_0_0_2"/>
    <dbReference type="OMA" id="GITHTTM"/>
    <dbReference type="OrthoDB" id="119984at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8429" checksum="CA148F03EC283256" modified="2011-05-03" version="1">MVEETIEKYEMRIPPGITGQIVAHVMEEFDLEVKQSDFGPVFLGEMKNLEDARDYIVKELNSRIAELENKKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA22</accession>
    <name>F0TA22_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">YbhB YbcL family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644196.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0594"/>
    <dbReference type="EnsemblBacteria" id="ADZ08845">
        <property type="protein sequence ID" value="ADZ08845"/>
        <property type="gene ID" value="Metbo_0594"/>
    </dbReference>
    <dbReference type="GeneID" id="10277039"/>
    <dbReference type="KEGG" id="mel:Metbo_0594"/>
    <dbReference type="eggNOG" id="arCOG04702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_3_2_2"/>
    <dbReference type="OMA" id="LAWDEVP"/>
    <dbReference type="OrthoDB" id="98388at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="PTHR30289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="SSF49777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00481">
        <property type="entry name" value="TIGR00481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="17137" checksum="7DB1D328613129D8" modified="2011-05-03" version="1">MSIKITSQKFKEGEEIPDRYACKGVNVSPPLEWSPVADAVKYAIICEDPDAAQGIWTHWVIFNIPGELHAVDEWIMEREETKNGAKQGLNDFGTVGYRGPCPPDGTHRYYYRIYALDSEIELPSMITRSQLKKAMEGHIIDEGSLMGRYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA41</accession>
    <name>F0TA41_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-flanked domain DUF304</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645342.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1768"/>
    <dbReference type="EnsemblBacteria" id="ADZ09991">
        <property type="protein sequence ID" value="ADZ09991"/>
        <property type="gene ID" value="Metbo_1768"/>
    </dbReference>
    <dbReference type="GeneID" id="10278225"/>
    <dbReference type="KEGG" id="mel:Metbo_1768"/>
    <dbReference type="eggNOG" id="arCOG04619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094459_0_0_2"/>
    <dbReference type="OrthoDB" id="92108at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="2">
        <location>
            <begin position="90"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="22624" checksum="92A12DAD728BBA7B" modified="2011-05-03" version="1">MSMRRPTHPGEKILLKTRPQFISTLESAFIRLIILLLLLYFFTAIIGIFAAIQGRIDYLTTVPFVQYSTYVLLIIIAVLFFWILWNFLSWRSTWYTVTTQRVQIKSGVLSTKSVYIHFNKMQDIEVTQSLVQRISSSGDIEIFGGRDKTNLILEDIPKPNQVEDLINQRIEVLSREPESRASKRKERYDDERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA49</accession>
    <name>F0TA49_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645350.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09999">
        <property type="protein sequence ID" value="ADZ09999"/>
        <property type="gene ID" value="Metbo_1776"/>
    </dbReference>
    <dbReference type="GeneID" id="10278233"/>
    <dbReference type="KEGG" id="mel:Metbo_1776"/>
    <dbReference type="HOGENOM" id="CLU_2091318_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="14145" checksum="C9324248001653A7" modified="2011-05-03" version="1">MLEYNYSHHKLPPSLPGDLRIHINFIDNEIIDPEEKLYLDLKTQNYISKVSKYDIEMNYNVNHEINYFEIEFILNYAVYTTNFYSIYTKALTHLKKILENTYFPYYQELRNSFIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA9</accession>
    <name>F0TAA9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">30S ribosomal protein S28e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps28e</name>
        <name evidence="4" type="ordered locus">Metbo_0610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644212.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0610"/>
    <dbReference type="EnsemblBacteria" id="ADZ08861">
        <property type="protein sequence ID" value="ADZ08861"/>
        <property type="gene ID" value="Metbo_0610"/>
    </dbReference>
    <dbReference type="GeneID" id="10277055"/>
    <dbReference type="KEGG" id="mel:Metbo_0610"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="OMA" id="MHGEASQ"/>
    <dbReference type="OrthoDB" id="121548at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_S28e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="PTHR10769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7655" checksum="0759ACC6147E5282" modified="2011-05-03" version="1">MEDGTPAEIIEVLKRTGMTGEVMQVKCRILDGRDKGRILTRNVMGAIREGDILMLLDTIREAKEIRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB4</accession>
    <name>F0TAB4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="7">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trpS</name>
        <name evidence="9" type="ordered locus">Metbo_0615</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644217.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0615"/>
    <dbReference type="EnsemblBacteria" id="ADZ08866">
        <property type="protein sequence ID" value="ADZ08866"/>
        <property type="gene ID" value="Metbo_0615"/>
    </dbReference>
    <dbReference type="GeneID" id="10277060"/>
    <dbReference type="KEGG" id="mel:Metbo_0615"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_3_0_2"/>
    <dbReference type="OMA" id="SIYHRFM"/>
    <dbReference type="OrthoDB" id="33997at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="70"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="365" mass="41279" checksum="1C0C3665243099C2" modified="2011-05-03" version="1">MIDPWSSAVVDYENLIEQFGIKPFADLLPEVDDPPVLMKRGIIFGQRDFGRIVKAMHEKSEFAVVSGMMPSGKMHIGHKMIVDQLVWYQKHGADIFIPIADMESFSARDIEFKESRELAIVEYLTNYIALGLDFKQKNIHCYLQSENKTVQSLAYLTGKRVNLNEMKAIYGFSGSTNISHLYVPLVQVADILHPQLEEFGGPKPTVVPVGPDQDPHIRLTRDIADRLKSKFNFIAPSSTYHRFITGLTGDKMSSSKPKTAIFLSDKPEVAEKKIKTAKTGGRESLDEQKKLGGIPEDCVVYEMLLYHLVESDKELVEMYHDCKNGTMMCGECKAKASTMIRIFFKDLSKRRNVAEGEARKILSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD5</accession>
    <name>F0TAD5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Replication factor C large subunit</fullName>
            <shortName evidence="4">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rfcL</name>
        <name evidence="7" type="ordered locus">Metbo_0636</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644238.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0636"/>
    <dbReference type="EnsemblBacteria" id="ADZ08887">
        <property type="protein sequence ID" value="ADZ08887"/>
        <property type="gene ID" value="Metbo_0636"/>
    </dbReference>
    <dbReference type="GeneID" id="10277081"/>
    <dbReference type="KEGG" id="mel:Metbo_0636"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_0_2"/>
    <dbReference type="OMA" id="GGWTRYG"/>
    <dbReference type="OrthoDB" id="24257at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="37"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="403"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="491" mass="55828" checksum="34136F5745BE427B" modified="2011-05-03" version="1">MLWTEKYSPKNFDEVLGNVKIKDEILNWTEDWLLGKTSQCILLIGPPGTGKTTMAHLAANEFSEYIELNASDKRSYDILKNTIGESSLSKSLYGDGLKLIILDEVDGIHGNEDRGGTRAINQIVKDGKHPIIMMANDPYSKRLQSLKPKCNTLIMRKVHTNSIVSLLKKICVKEGVEFEEHVIRNLAKRSNGDLRTAINDLEVIARGQERITSEDLELLSSKDDIVNIFDTVRTVLKSKDPKRVRAAMRVDSEPSFILEIITENIPKEYEKVNEIQSAYEKISQADLYLGRAFNTRIYTYWKYAYELMGLGVALSKAETYKKFSRLTNSSIFPKLSKSRGNRNLEDKVAEKMGHKLHASKKVVKEQFPYMKIMFQNDETAHELATYFDLADDEVKLFRSRKIKVKKSKKKPTKSNKSATSISTPKLSGDPKVETSKKVDVEAKVSSKKKSRSKKVKNEDKAVKKSSKDTENENDSEKTSKDSQMSLFNFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF5</accession>
    <name>F0TAF5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0656</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644258.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0656"/>
    <dbReference type="EnsemblBacteria" id="ADZ08907">
        <property type="protein sequence ID" value="ADZ08907"/>
        <property type="gene ID" value="Metbo_0656"/>
    </dbReference>
    <dbReference type="GeneID" id="10277101"/>
    <dbReference type="KEGG" id="mel:Metbo_0656"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="105856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="27146" checksum="C02780E2035C8066" modified="2011-05-03" version="1">MTRVITVASGKGGVGKTTITANLGVALSTYGEETIVLDADVAMANLELILGMEGKSVTLHDVLSGAASIEDAIYEGPGGVKVVPAGISLEGLRKIKMDRLESALEILVETADILLIDAPAGLEKDALAAIAAAQEMILVTTPEVPSISDALKTKIIANKLGVDIIGVVINREQHDKTFLTISEIETILEVPVIAVIPDDPEVSRAAAFGEPLIIKNPKSPTSNAIMQLGADLIGEEYQPIEPDKKGVISKLVEGLLGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK2</accession>
    <name>F0TAK2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645425.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1852"/>
    <dbReference type="EnsemblBacteria" id="ADZ10074">
        <property type="protein sequence ID" value="ADZ10074"/>
        <property type="gene ID" value="Metbo_1852"/>
    </dbReference>
    <dbReference type="GeneID" id="10278309"/>
    <dbReference type="KEGG" id="mel:Metbo_1852"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_570634_0_0_2"/>
    <dbReference type="OrthoDB" id="101258at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="129"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="478" mass="53276" checksum="7112428814E95678" modified="2011-05-03" version="1" precursor="true">MKKQFIFVSALIFVFLIIGTVSSASASDTLQPKVKSINPTNNSFNVPTYHNLTIKFTEPVKAGNNYITLKNSKGTSVQFKKIISGNTLTIKPTSNLSRGVKYVLYLHTGCVKDLSGNNISIFKSCFTTDSTGPKLTSIDPVKNAFNVNLNKTISLKFTEAIKFGNNQIELVKSNGAHVSFTKMIKGNALYIKPQSLSSGTKYFLTFHTNSLKDISGNGVAYYKSYFVTSSGLNTNHPLSTVKICFIHHSSGGNWLSNGNGNLGAALNRNNYYVTETDYGWSAEDGDNLGDRTDTVNWPEWFKDRKMSHVYQNNYHSAYTNTIGNPGGENDIIIFKSCFPNSKVGSSIDDEKEIYNSLKTYFAAHPNKLFVLITPPGETQVSSYKLTKDLCNWLVDKDGWLKGYTGKNVLVYDFYGTLSETNSHHRYYNGVIQHVYASDYDGISPYHDGDDHPNALGNQKATNEFITLLNIAYNRWKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN1</accession>
    <name>F0TAN1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645454.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1882"/>
    <dbReference type="EnsemblBacteria" id="ADZ10103">
        <property type="protein sequence ID" value="ADZ10103"/>
        <property type="gene ID" value="Metbo_1882"/>
    </dbReference>
    <dbReference type="GeneID" id="10278340"/>
    <dbReference type="KEGG" id="mel:Metbo_1882"/>
    <dbReference type="eggNOG" id="arCOG00349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_9_0_2"/>
    <dbReference type="OMA" id="MCISCGN"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="87" mass="9458" checksum="180B929FEC3D85E7" modified="2011-05-03" version="1">MNSGNSDDNPKFLVVVERDKCTSCGTCEDACPELFELDSESLAHIKGSKRVGNNDELGVETEDCSLDAAESCPVMCIHVYEDGEELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP3</accession>
    <name>F0TAP3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1895</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645466.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1895"/>
    <dbReference type="EnsemblBacteria" id="ADZ10115">
        <property type="protein sequence ID" value="ADZ10115"/>
        <property type="gene ID" value="Metbo_1895"/>
    </dbReference>
    <dbReference type="GeneID" id="25394754"/>
    <dbReference type="KEGG" id="mel:Metbo_1895"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="SIIMDIM"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="258"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="493"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="536" mass="61722" checksum="A543EA6433022BFA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ8</accession>
    <name>F0TAQ8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methanogenesis marker protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644285.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0683"/>
    <dbReference type="EnsemblBacteria" id="ADZ08934">
        <property type="protein sequence ID" value="ADZ08934"/>
        <property type="gene ID" value="Metbo_0683"/>
    </dbReference>
    <dbReference type="GeneID" id="10277128"/>
    <dbReference type="KEGG" id="mel:Metbo_0683"/>
    <dbReference type="eggNOG" id="arCOG00640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073250_0_0_2"/>
    <dbReference type="OMA" id="VPMVGGH"/>
    <dbReference type="OrthoDB" id="57531at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02192">
        <property type="entry name" value="PurM-like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017668">
        <property type="entry name" value="Methan_mark_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011413">
        <property type="entry name" value="UCP036540_AIR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036540">
        <property type="entry name" value="UCP036540_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03267">
        <property type="entry name" value="methan_mark_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="62"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="188"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="35547" checksum="5A5C5809D2BD1813" modified="2011-05-03" version="1">MNLDTLVNSIKTYEGITRKNFIKGATKILDDTYNIAGRTALGFGDDASAIDIGNDKYLLMAADGMWGQLMEGNPQWAGYCSVLVNVNDIAAMGGIPMGMTNVISVNDPELCNEIMIGIKEGVKKFGVPMVGGHLHPDTPYLALDVSITGIVGKEDVITSAGAEVGDVVLVAMDLDGQQHPDFKLNWDTTTHKTAELVQAQIMAMNELGKKHLLTAGKDVSNPGIIGTLGMLLEASEVGATIELGKIPRNSTVDWEEWLKVYPGSGFVLTAKEENAEECIKILEKVNITTQVAGRIIEEKLLNLTHQGQERTVFDFNKDMIIGIKEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR5</accession>
    <name>F0TAR5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FeMo cofactor biosynthesis protein NifB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nitrogenase cofactor maturase NifB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Radical SAM assemblase NifB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_0690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644292.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0690"/>
    <dbReference type="EnsemblBacteria" id="ADZ08941">
        <property type="protein sequence ID" value="ADZ08941"/>
        <property type="gene ID" value="Metbo_0690"/>
    </dbReference>
    <dbReference type="GeneID" id="10277135"/>
    <dbReference type="KEGG" id="mel:Metbo_0690"/>
    <dbReference type="eggNOG" id="arCOG00956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027639_1_0_2"/>
    <dbReference type="OMA" id="PCYNEKL"/>
    <dbReference type="OrthoDB" id="46264at2157"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005980">
        <property type="entry name" value="Nase_CF_NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="PTHR43787:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="21"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="31552" checksum="5BFF7342DD733082" modified="2011-05-03" version="1">MNESRFAHITKVHPCFNEKMHDKVGRIHVPIAPKCNIQCNFCTREINKCEQRPGVASRVMTVDQAVEHVRKVTTEMPIAVVGVAGPGDALFNEETFEFFKRMDEEFPDLIKCMSTNGLLLPEKADEIAALHINTVTVTVNAIDPEIGTQIYSNVYFDKKLYKGEEGFNVLVKNQLEGIKKLAENGVVVKVNSVLIPGVNDKHILDIARKVKEQGASLMNVIPLIPMHKFKDTPKPDCAALSEVRDAVEEIIPVFRACTQCRADAYGVPGKEDKHLDMTPASHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT3</accession>
    <name>F0TAT3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin/nitric oxide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644310.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0708"/>
    <dbReference type="EnsemblBacteria" id="ADZ08959">
        <property type="protein sequence ID" value="ADZ08959"/>
        <property type="gene ID" value="Metbo_0708"/>
    </dbReference>
    <dbReference type="GeneID" id="10277153"/>
    <dbReference type="KEGG" id="mel:Metbo_0708"/>
    <dbReference type="eggNOG" id="arCOG00509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="OMA" id="TIGGHAP"/>
    <dbReference type="OrthoDB" id="72715at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="259"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="45498" checksum="E3BBA9C79C0C9169" modified="2011-05-03" version="1">MKANAIKITEGVYWVGVLDWDLRDYHGYTLDGTTYNAYLVFGEKVALIDNTYPGTSAQLWARMEDAFKKENREFKLDLIIQNHVEKDHSGALAEICRKFPGIPVYCTAAAVKGLKMHYSSLEDVNFNVVKTGDSLDIGGKEFAFLEAQMLHWPDSMFSLLLEDGILFSNDAFGQHLCFKDRYDTDISENVLMHAAAKFYANLLTPLSGLVLNKFKQVNSLDLLGKIQMIAPSHGQIWTDPMKIIDAYTGWATGKCSNKITLVYDTMHGSTQLMSQAFAEGIMSEDVDVAMHFLHSDERSNIVTNILDSKAVVFGVPTMFNQVYPSIGDLIFYLKGLNFARTGLKRKAITFGSMGWSGEAPKILAQELENCGFEVVNQMKVNYKPSEDELEKCYEMGVDLAKLVKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU3</accession>
    <name>F0TAU3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="2">ITPase/XTPase</shortName>
            <ecNumber evidence="2">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="2">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0718</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + ITP = H(+) + IDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + XTP = H(+) + phosphate + XDP</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644320.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0718"/>
    <dbReference type="EnsemblBacteria" id="ADZ08969">
        <property type="protein sequence ID" value="ADZ08969"/>
        <property type="gene ID" value="Metbo_0718"/>
    </dbReference>
    <dbReference type="GeneID" id="10277165"/>
    <dbReference type="KEGG" id="mel:Metbo_0718"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087417_0_1_2"/>
    <dbReference type="OMA" id="ADYWVGI"/>
    <dbReference type="OrthoDB" id="101951at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00258">
        <property type="entry name" value="TIGR00258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTPase_I-T" evidence="3">
        <location>
            <begin position="6"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="176" mass="19337" checksum="B6AFFFF5A162EB2E" modified="2011-05-03" version="1">MKVVVGSKNPVKVNAARNVLKRIYNDLTVHSLNVDSEIPDQPFGLDQTIEGAVNRAKNAFSDEFDLSVGIESGLIEVPQTLTGYIDIQWCAIFDGNLITIGASSGFEYPPTVIKRVMEGFEVGDVMDEVTGVDDLGQKKGAVSHLSHDMLNRTENTEECVLTAMIPRMNEKVYGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX3</accession>
    <name>F0TAX3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0748"/>
    <dbReference type="EnsemblBacteria" id="ADZ08999">
        <property type="protein sequence ID" value="ADZ08999"/>
        <property type="gene ID" value="Metbo_0748"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0748"/>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_14_2_2"/>
    <dbReference type="OMA" id="IECYDEV"/>
    <dbReference type="OrthoDB" id="112387at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="158" mass="17904" checksum="E4D6986D671E5572" modified="2011-05-03" version="1">MSKLSGEWNNEGIEFYENGQYPEALKCYEKALELDKNFVQALNNKGNVLRVLGRYDESLESFDTAILKEPKNAELWVNKGAVYYEMDDYNQAIECYDEALKIEPKNTNALSNKGAALANSGDLDSGMECFNEALKIDPLDVNAHINRDKLKDFMDNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX9</accession>
    <name>F0TAX9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter, inner membrane subunit CbiQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644356.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0754"/>
    <dbReference type="EnsemblBacteria" id="ADZ09005">
        <property type="protein sequence ID" value="ADZ09005"/>
        <property type="gene ID" value="Metbo_0754"/>
    </dbReference>
    <dbReference type="GeneID" id="10277202"/>
    <dbReference type="KEGG" id="mel:Metbo_0754"/>
    <dbReference type="eggNOG" id="arCOG02249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_5_0_2"/>
    <dbReference type="OMA" id="IAMMMYR"/>
    <dbReference type="OrthoDB" id="51220at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="29982" checksum="B60B88C53E98719B" modified="2011-05-03" version="1">MFELTLDGYAHSNELRNLNPYFKVLFAIITMIVSLISTSPVVPLFIFLFFTFLILFEAKIPIKFYLKFLLVPLTFAFITFVFMAIFFGYGQYVYDLGFLNLGVSADGFNRAALVFARIMGGFACLAFLALTTPMTELFAILEAIKIPKIVVELTMLMYRYIFLFLDEAVNMYHSQETRNGYSTMKKSYKSMGMLISNLFIKTWLKGEQTFIAMESRCYDGSIKTMNDFGSVKTIGIKKFSFLIMFEIVLCAAVYFSAGFHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ4</accession>
    <name>F0TAZ4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645491.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1920"/>
    <dbReference type="EnsemblBacteria" id="ADZ10140">
        <property type="protein sequence ID" value="ADZ10140"/>
        <property type="gene ID" value="Metbo_1920"/>
    </dbReference>
    <dbReference type="GeneID" id="10278378"/>
    <dbReference type="KEGG" id="mel:Metbo_1920"/>
    <dbReference type="eggNOG" id="arCOG12324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346404_0_0_2"/>
    <dbReference type="OMA" id="WDYLHEM"/>
    <dbReference type="OrthoDB" id="79090at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="23516" checksum="21981C5206F7C320" modified="2011-05-03" version="1">MDYDLGEKGVDTKKLALESLNNTELFKELIIGIRSKDKTVRTNSFNALMVVADNNGDILYPKWDYLHEMLVSNNSNEQYVAIYLLANLTASDTENKFETIFDDYFSLLGGEKTLPASHVILSSGKIIENKPELRSKIISNLLSTDETFKGRQKDLLKVYVIETLRKIPSDPGDKDRIEEFIKSQLNSSSSRTRSAAKCYVERCFLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ7</accession>
    <name>F0TAZ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1923</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1923"/>
    <dbReference type="EnsemblBacteria" id="ADZ10143">
        <property type="protein sequence ID" value="ADZ10143"/>
        <property type="gene ID" value="Metbo_1923"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1923"/>
    <dbReference type="eggNOG" id="arCOG04846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159088_0_0_2"/>
    <dbReference type="OMA" id="MGSEVCD"/>
    <dbReference type="OrthoDB" id="124865at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019209">
        <property type="entry name" value="DUF2098"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017099">
        <property type="entry name" value="UCP037053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09871">
        <property type="entry name" value="DUF2098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037053">
        <property type="entry name" value="UCP037053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="11972" checksum="625462FEDB44370F" modified="2011-05-03" version="1">MEDVRGIPITKGNHVRYTGTGSTGEVTDLKSDDEGSWVLLDTTELWYKTDSLEVIREEDYLKIKNHTIFKSEKVNEKEDVKDKVQRSKTKFENVDMSNELCDGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB09</accession>
    <name>F0TB09_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645506.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1935"/>
    <dbReference type="EnsemblBacteria" id="ADZ10155">
        <property type="protein sequence ID" value="ADZ10155"/>
        <property type="gene ID" value="Metbo_1935"/>
    </dbReference>
    <dbReference type="GeneID" id="10278393"/>
    <dbReference type="KEGG" id="mel:Metbo_1935"/>
    <dbReference type="eggNOG" id="arCOG03505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363664_0_0_2"/>
    <dbReference type="OMA" id="KIASWIW"/>
    <dbReference type="OrthoDB" id="107986at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021338">
        <property type="entry name" value="DUF2953"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11167">
        <property type="entry name" value="DUF2953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="196" mass="22781" checksum="1476E49085FEE7E9" modified="2011-05-03" version="1">MITILIIVVLVVVFIIACLLMVPFHIILNLENQGLEFKGIMQIKWMKIGIIKREIPSETPEEEKEEKKEEKGEAAKWNLDKIVKVFNLFLESLPHFERILVALYRSIDLERFWLNLRVGLDSPVDTAMIAGVFWSMNSIINTIPRVTVTLNPQFMKPVFEGKVEIEFKIRLLWIVAESIRAVTKKPVRNLIREVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB37</accession>
    <name>F0TB37_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein Metbo_1963</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645534.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1963"/>
    <dbReference type="EnsemblBacteria" id="ADZ10183">
        <property type="protein sequence ID" value="ADZ10183"/>
        <property type="gene ID" value="Metbo_1963"/>
    </dbReference>
    <dbReference type="GeneID" id="10278423"/>
    <dbReference type="KEGG" id="mel:Metbo_1963"/>
    <dbReference type="eggNOG" id="arCOG01336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="OMA" id="RGCIGFP"/>
    <dbReference type="OrthoDB" id="107157at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="PTHR13016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="SSF143447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00296">
        <property type="entry name" value="TIGR00296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21431" checksum="F50A59784B107DC5" modified="2011-05-03" version="1">MLSEEQGQFLLKLAREAITVYLKEGRVIPIPETTDTIMHENRGVFVTLNKENCLRGCIGYPEPVMPLINAVVDAAISAAVRDPRFNCVTLEELETIELELSVLTKPELIPVDDPTEYLDNIIIGEDGLIVESGPYRGLLLPQVATEWGWNKEEFLSNTCNKAGLNSECWLSGDVKIYKFSSEIFEEDLKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB94</accession>
    <name>F0TB94_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl18</name>
        <name evidence="6" type="ordered locus">Metbo_0794</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644396.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0794"/>
    <dbReference type="EnsemblBacteria" id="ADZ09045">
        <property type="protein sequence ID" value="ADZ09045"/>
        <property type="gene ID" value="Metbo_0794"/>
    </dbReference>
    <dbReference type="GeneID" id="10277242"/>
    <dbReference type="KEGG" id="mel:Metbo_0794"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="OMA" id="MAHGPRY"/>
    <dbReference type="OrthoDB" id="108717at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_L18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_L5_euk/L18_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="PTHR23410"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="21218" checksum="955CE6606443A4B1" modified="2011-05-03" version="1">MAQSSRYKLAFRRRREGKTDYKARLNLIGLDKSRMVVRITNQHTIAQIINVGENGDETVVSAHSKEVQKIGWIGSGKNTSGAYLTGYLLAKKALDAGIEAAVLDIGLKTNIKGARIYAALKGAVDGGLQVPHNDVVLPSDERIRGEHVANYAESLTDEEVEKRFSGYIKNGLSPKDLPDHFESIKNKIEEGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB5</accession>
    <name>F0TBB5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644417.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0815"/>
    <dbReference type="EnsemblBacteria" id="ADZ09066">
        <property type="protein sequence ID" value="ADZ09066"/>
        <property type="gene ID" value="Metbo_0815"/>
    </dbReference>
    <dbReference type="GeneID" id="10277264"/>
    <dbReference type="KEGG" id="mel:Metbo_0815"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="ACPAKIL"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="6960" checksum="7B51C6A5E6EC6A2D" modified="2011-05-03" version="1">MVKITINHEKCDGADCAECIDVCPMEILILDGDKVVIQNKEECSLCEVCMDVCPNEAINVEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC7</accession>
    <name>F0TBC7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin/nitric oxide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644429.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0827"/>
    <dbReference type="EnsemblBacteria" id="ADZ09078">
        <property type="protein sequence ID" value="ADZ09078"/>
        <property type="gene ID" value="Metbo_0827"/>
    </dbReference>
    <dbReference type="GeneID" id="10277276"/>
    <dbReference type="KEGG" id="mel:Metbo_0827"/>
    <dbReference type="eggNOG" id="arCOG00524">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094839_1_0_2"/>
    <dbReference type="OMA" id="ADTNIEY"/>
    <dbReference type="OrthoDB" id="82365at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026816">
        <property type="entry name" value="Flavodoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12724">
        <property type="entry name" value="Flavodoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="176" mass="20080" checksum="CD2E85A2B1A1D7FD" modified="2011-05-03" version="1">MKALVIYGSRYGTAEEISERIIDILQKENVDVDLINSEKSQSVKVENYDLVVAGSGIKMGKWTKNTLNFLKKNRKELSNRKVALFVSCGAANQEKNRAEAQEKYLDNIAAKYLDTEPVAKGLFGSVYDPNADHGLLYKLIKGSIEKEMIKMGQDPTQKHDYRDWDYINQWTIDLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF8</accession>
    <name>F0TBF8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0597 protein Metbo_2008</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-cysteine = H(+) + hydrogen sulfide + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24931"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="EC" id="4.4.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the L-cysteine desulfidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UPF0597 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645578.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2008"/>
    <dbReference type="EnsemblBacteria" id="ADZ10227">
        <property type="protein sequence ID" value="ADZ10227"/>
        <property type="gene ID" value="Metbo_2008"/>
    </dbReference>
    <dbReference type="GeneID" id="10278468"/>
    <dbReference type="KEGG" id="mel:Metbo_2008"/>
    <dbReference type="eggNOG" id="arCOG05065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051840_0_0_2"/>
    <dbReference type="OMA" id="GAMIPVM"/>
    <dbReference type="OrthoDB" id="27747at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01845">
        <property type="entry name" value="UPF0597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021144">
        <property type="entry name" value="UPF0597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501">
        <property type="entry name" value="PTHR30501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006054">
        <property type="entry name" value="UCP006054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_alpha" evidence="8">
        <location>
            <begin position="85"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="436" mass="46313" checksum="FE9E9067A203E807" modified="2011-05-03" version="1">MLEEQFFEILNRELVVAMGCTEPIAIVIAAAHAKKHSKGSIEAVNVKASRNIVKNAFSVKIPGTDDCGINLAAALGMYIGASEINMEVLEDIKVEEVDAAKKMVEDGLITVELADTPKKLYVEVTVQTDRSISKAVVEDFHDNLVLVEVDGKKLDVKNSVKPMKNDASMFEDLTLDEILRFVTEVDTDKLGLIKQSIDLNSKISLEGINNCYGIKVGKNIQTNIGGDIFGNDICNHAVALTASGSDARMAGCNLPVMTNSGSGNQGISATTPVVVFGAYVHADEETLIRAVALSNLITIYIKSSLGRLSALCGATVASAGSCCGITYLMGGNNDQIKSALQNILGNVTGIICDGAKAGCALKVATCTTAAIQSVICVMEGQHIQSTDGIVEEDPEDTIKNFCKIGQVGMAEADKIILEIMMDKSNKQKADLESDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL0</accession>
    <name>F0TBL0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0837"/>
    <dbReference type="EnsemblBacteria" id="ADZ09087">
        <property type="protein sequence ID" value="ADZ09087"/>
        <property type="gene ID" value="Metbo_0837"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0837"/>
    <dbReference type="eggNOG" id="arCOG00187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077076_0_0_2"/>
    <dbReference type="OMA" id="CCEICVR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="240"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="33634" checksum="EA2221830CE3D5D6" modified="2011-05-03" version="1">MLKNKLFVVGLITMDIKIYIVSSGEYGLRIINNIAKNGFAASIVGMHEFPDDLPEFIDNPEIYIPGTIPESDLILALGLKGDINLTLPIIASKAGAKAVISEIHDPAQLPPGLQREVEESAGDIKIVFAKPFCSLKPVGDDVVDEFAHYFGKPELKIESNKLIKNVEVICGAPCGSTQFVADELEGFPVEDAELESGNKIHNYPCIASMATDPVVGDTILHLAGYKIKEAVKVGVGFATKSAVVDKDACMGGIDCDHNCIDVCPNVKIGDDTVTVDEEKKAVINPVSCGCCEICVRECPYGAIEIVEEKIDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM0</accession>
    <name>F0TBM0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0847"/>
    <dbReference type="EnsemblBacteria" id="ADZ09097">
        <property type="protein sequence ID" value="ADZ09097"/>
        <property type="gene ID" value="Metbo_0847"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0847"/>
    <dbReference type="HOGENOM" id="CLU_3362564_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="35" mass="3806" checksum="F635AB3CF2136E54" modified="2011-05-03" version="1">MKKVYKKPELKEHGDIKSLTKGGDIGGFDQDNAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP5</accession>
    <name>F0TBP5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09122">
        <property type="protein sequence ID" value="ADZ09122"/>
        <property type="gene ID" value="Metbo_0873"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0873"/>
    <dbReference type="eggNOG" id="arCOG13998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120322_0_0_2"/>
    <dbReference type="OMA" id="MFTAINV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="22954" checksum="2E01BCE2B8AEB17C" modified="2011-05-03" version="1">MEITIKQLKKYFEVFLCKIYTRNIPYSLDMRIENVYKKIFGLNYWGLIKMMTVAIVKRKLKPGKTYEDFRKAWYHTVGFGARTRMFTAINVFDPTEIIVMGFVESEMDEFMDGLKIDVNERLENPLDDIIEPEIVRQFGMVVSEDDFSPAGAIEYQDASINNEKTDLKRVYEDISKIAQMIQVASEDRDKRKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP9</accession>
    <name>F0TBP9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644477.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0877"/>
    <dbReference type="EnsemblBacteria" id="ADZ09126">
        <property type="protein sequence ID" value="ADZ09126"/>
        <property type="gene ID" value="Metbo_0877"/>
    </dbReference>
    <dbReference type="GeneID" id="10277326"/>
    <dbReference type="KEGG" id="mel:Metbo_0877"/>
    <dbReference type="eggNOG" id="arCOG06480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848915_0_0_2"/>
    <dbReference type="OMA" id="THSHVFK"/>
    <dbReference type="OrthoDB" id="61252at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="339" mass="37348" checksum="4DE1517C19E8A34F" modified="2011-05-03" version="1">MKFNKVNILGILMILFAVLLVFTNFLDKIILMLTYHFLEGSSQGKSIILFTVMGSILLLYPLFEVNGFIGSKISNLGSIFKFEPKKYLKITICLLILTYLVGMLTELLIRYKFGVSPFNTFIAYNSNSYSSTALTHSHVFKSMLGLVISNLGIHINAGINAGVALIPYTLPLALIVFVTYPLIYIGGLIAVANEKRFLYQILLAFAVTVSLIGVLDGGLFSTPALVGLATLLGIYFIKKPFKAKDLIKPSVIIIILIILRVSLGILGTNTVSHEITILNPSDHIDLQSYNVTSIQKLDNKTVYSVPGNTNDKVLLMGLTRDLKGNGTGFFLSWNIYSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU2</accession>
    <name>F0TBU2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-inosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">SIHH</shortName>
            <ecNumber evidence="4">3.13.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + S-inosyl-L-homocysteine = inosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:59828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645635.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2065"/>
    <dbReference type="EnsemblBacteria" id="ADZ10284">
        <property type="protein sequence ID" value="ADZ10284"/>
        <property type="gene ID" value="Metbo_2065"/>
    </dbReference>
    <dbReference type="GeneID" id="10278527"/>
    <dbReference type="KEGG" id="mel:Metbo_2065"/>
    <dbReference type="eggNOG" id="arCOG04137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_0_2_2"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="OrthoDB" id="17629at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="6">
        <location>
            <begin position="184"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="213"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="292"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="417" mass="46325" checksum="B5C9C0A37DFFC41E" modified="2011-05-03" version="1">MSNNVKDMSLAPEGIKKIEWVQKHMPVLEHIKKEFQETKPFEGVTIASCLHLEPKTINLGLTLQEGGAEVAMTGCNPLSTQDDATAAGASLGLNMYGWRGETNEEYYENLNKVLDHEPDIIIDDGADMIFLVHKERRDLMDKIIGACEETTTGIHRLKAMHADKALEFPVMAVNDSYMKYLFDNRYGTGQSTFDSIMGSTNVLIAGKTLVVCGYGWCGRGVAMRAAGLGANVIVTEIDPIRALEARMDGYRVMKIREAVKEADMIITVTGNIDVVSGDDFKYMKDGCIMANSGHFNVEINKQDLQKISKTTKLLKPDIEEFTTEDGRKLYLLADGRLVNLAGERGQGHPAEIMDMSFAMQSLSAKYLLENKLDVGVYKTLDETDIDVAKLKLNAMKIDIDSLTERQVAYLKNWDEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY5</accession>
    <name>F0TBY5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645678.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2108"/>
    <dbReference type="EnsemblBacteria" id="ADZ10327">
        <property type="protein sequence ID" value="ADZ10327"/>
        <property type="gene ID" value="Metbo_2108"/>
    </dbReference>
    <dbReference type="GeneID" id="10278571"/>
    <dbReference type="KEGG" id="mel:Metbo_2108"/>
    <dbReference type="eggNOG" id="arCOG10253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379452_0_0_2"/>
    <dbReference type="OrthoDB" id="121049at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="9867" checksum="9206DB45B949BFB2" modified="2011-05-03" version="1" precursor="true">MNPKKSGTLFIVLIIALLAFGVASAANVFGLGSNLIDSSIPSNLSLNGQQQISAIGDTSFTPVYVNKRVVVNITNTTPVVVPTNNSTNYTPTNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC36</accession>
    <name>F0TC36_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endonuclease III</fullName>
            <ecNumber evidence="9">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nth</name>
        <name evidence="11" type="ordered locus">Metbo_0940</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0940"/>
    <dbReference type="EnsemblBacteria" id="ADZ09187">
        <property type="protein sequence ID" value="ADZ09187"/>
        <property type="gene ID" value="Metbo_0940"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0940"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_2"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="OrthoDB" id="124734at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="10">
        <location>
            <begin position="39"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="24818" checksum="15AD6C00A4296863" modified="2011-05-03" version="1">MELIKISRIESIIVNLEDIYTLREFEDSDPFRVLIRTILSQRTRDENTDAASAMLFSKYSTPEEIANAPTEEVEKLIKKSGFYHVKASRVREVSRIIHEDYNDTVPEDMAELLSLPGVGRKTANCVLVYGFHKDAIPVDVHVHRISNRIGLVNTGTPDETEEKLMKIVPKKFWLPLNDLFVQFGQTICKPIGPKHEICPIAEYCDYYKNMKTTNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC51</accession>
    <name>F0TC51_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="10" type="ordered locus">Metbo_0955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644553.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0955"/>
    <dbReference type="EnsemblBacteria" id="ADZ09202">
        <property type="protein sequence ID" value="ADZ09202"/>
        <property type="gene ID" value="Metbo_0955"/>
    </dbReference>
    <dbReference type="GeneID" id="10277404"/>
    <dbReference type="KEGG" id="mel:Metbo_0955"/>
    <dbReference type="eggNOG" id="arCOG03054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060478_0_0_2"/>
    <dbReference type="OMA" id="TWLMNLG"/>
    <dbReference type="OrthoDB" id="63405at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="281" mass="29728" checksum="0A29771BA3016B5E" modified="2011-05-03" version="1">MSMMDKLLDSYKPLIIIPVIITLIALAIVATGGLQEGIDLKGGSIAVVQLEKPVSDDALAALVENGTGNQQVTVTTSGGNTATIEIAGGATDVIALTNALSGTATLQSFRAVGPSLSKQSLTQVYYALAFAFLFMSITVFIIFRNLIPSLAVIFAAFSDIIIAVGGMSLLGIPLSLASVGALLMLIGYSVDTDILLTTRVLKRREGTINERAKDAMKTGFTMAAAAIGSMVALYLVVRFFMPYAQVLDQIASVLIIGLVADVLATWLFNLGVLRWYMEGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC55</accession>
    <name>F0TC55_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylglutamate kinase</fullName>
            <ecNumber evidence="7">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NAG kinase</fullName>
            <shortName evidence="7">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argB</name>
        <name evidence="9" type="ordered locus">Metbo_0959</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644557.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0959"/>
    <dbReference type="EnsemblBacteria" id="ADZ09206">
        <property type="protein sequence ID" value="ADZ09206"/>
        <property type="gene ID" value="Metbo_0959"/>
    </dbReference>
    <dbReference type="GeneID" id="10277408"/>
    <dbReference type="KEGG" id="mel:Metbo_0959"/>
    <dbReference type="eggNOG" id="arCOG00862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_2"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="60029at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="60"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="31617" checksum="EDEF302A48C278CA" modified="2011-05-03" version="1">METVNILIEALPYIKKFHHKKVMIKYGGHAMIDSDAMSSTARDTVLLKYVGMEPMVVHGGGPEISRSMSKLGKEPKFIGGLRITDSETMDIVKMVLVGKINTDIVSKVCLHGGKGIGISGKDNQLIKANKKAPHVMVDNSTGEEITVDLGLVGEIESVNTEIMEMLAENNYIPIVSPIGVDEVGNTLNLNADTVAGDIAAKMEAEKLIILTDVPGILEDPKDPETLIKKVKVDEIKDLIDNGIVKDGMIPKVLTCVNAVENGVKSAHIIDGRIKHSVLLEIFTTKGIGTMITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC61</accession>
    <name>F0TC61_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nac</name>
        <name evidence="4" type="ordered locus">Metbo_0965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644563.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0965"/>
    <dbReference type="EnsemblBacteria" id="ADZ09212">
        <property type="protein sequence ID" value="ADZ09212"/>
        <property type="gene ID" value="Metbo_0965"/>
    </dbReference>
    <dbReference type="GeneID" id="10277414"/>
    <dbReference type="KEGG" id="mel:Metbo_0965"/>
    <dbReference type="eggNOG" id="arCOG04061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146475_0_0_2"/>
    <dbReference type="OMA" id="PRKMKQM"/>
    <dbReference type="OrthoDB" id="105165at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="HYPK_UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00264">
        <property type="entry name" value="TIGR00264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="3">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="117" mass="12997" checksum="F1EAF808DB0C2CD5" modified="2011-05-03" version="1">MLPGAGMNPKQLKQMQRAMKQMGMDNKDIKGVTEVVIKFKTKEIVITSPKVNLMDFMGQQTYQVSGKIVENKIETELVIPDDDVDLVSTQTGVSKEKALETLKETKGDLAEAILRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC97</accession>
    <name>F0TC97_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645715.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10364">
        <property type="protein sequence ID" value="ADZ10364"/>
        <property type="gene ID" value="Metbo_2147"/>
    </dbReference>
    <dbReference type="GeneID" id="10278610"/>
    <dbReference type="KEGG" id="mel:Metbo_2147"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_1_0_2"/>
    <dbReference type="OMA" id="AYAEFVH"/>
    <dbReference type="OrthoDB" id="82596at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="18296" checksum="6319DF0C647F4778" modified="2011-05-03" version="1">MDSIDNLKEAFAGESQANRKYLAFAKKADEEGLFQVARLFRAAAQAETVHANNHLTVLKGVKSTEENLKEAIAGEVEEFKEMYPAFIKVAEEENNKKAHWTFDVANQVEEIHAELYQKALDSIGENVETIYYVCNFCGNTVEKEAPDICSICGAPKVEFKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA4</accession>
    <name>F0TCA4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645722.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2156"/>
    <dbReference type="EnsemblBacteria" id="ADZ10371">
        <property type="protein sequence ID" value="ADZ10371"/>
        <property type="gene ID" value="Metbo_2156"/>
    </dbReference>
    <dbReference type="GeneID" id="10278619"/>
    <dbReference type="KEGG" id="mel:Metbo_2156"/>
    <dbReference type="HOGENOM" id="CLU_1860717_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="16221" checksum="D61E3BE5DF831A5F" modified="2011-05-03" version="1">MYQVADFKKLKTLEDKIKEEYDLYSVIVVDKPLQRYSEVVSVPEKLYNITNAVFPNMRVLDHERYVYEDGKHIFYTEPYNPIRIRRINTKMAERFEVELIPLKRSFHDSTTFPYKIVIGKPGSLEIPDISIGWKGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF1</accession>
    <name>F0TCF1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645769.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2204"/>
    <dbReference type="EnsemblBacteria" id="ADZ10418">
        <property type="protein sequence ID" value="ADZ10418"/>
        <property type="gene ID" value="Metbo_2204"/>
    </dbReference>
    <dbReference type="GeneID" id="10278668"/>
    <dbReference type="KEGG" id="mel:Metbo_2204"/>
    <dbReference type="eggNOG" id="arCOG14017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405989_0_0_2"/>
    <dbReference type="OMA" id="FLDENCK"/>
    <dbReference type="OrthoDB" id="103378at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042425">
        <property type="entry name" value="APCDD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31021">
        <property type="entry name" value="PTHR31021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="21852" checksum="2B4D833AE3A80875" modified="2011-05-03" version="1">MLETQDLQGKWQSPEIETVNNPDGSVMYIQREATFQGDEWGIRIRSYLDKNGKEPFFTIHAKGNYILSGESSYVNNTTCVDFNNTGRYVTAHHTALVDMLNEVSVDTEWNLGEEHDVSITGCALVPSIDNCPVEYDIIQIEDNKIYFGEFTQDQQEDIQKLSRGDVEPSEDSAGICSVDNRPRQLMSYPLVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH1</accession>
    <name>F0TCH1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferroxidase</fullName>
            <ecNumber evidence="2">1.16.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.16.3.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644599.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1001"/>
    <dbReference type="EnsemblBacteria" id="ADZ09248">
        <property type="protein sequence ID" value="ADZ09248"/>
        <property type="gene ID" value="Metbo_1001"/>
    </dbReference>
    <dbReference type="GeneID" id="10277450"/>
    <dbReference type="KEGG" id="mel:Metbo_1001"/>
    <dbReference type="eggNOG" id="arCOG01095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065681_1_2_2"/>
    <dbReference type="OMA" id="CEDKGFE"/>
    <dbReference type="OrthoDB" id="87619at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="PTHR11431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="19236" checksum="B47E96611F9AB35C" modified="2011-05-03" version="1">MDEKMVESLNSQLNAEMYSAYLYLSMGAYFEDLDLGGFANWMRVQAQEEMTHAMKIHDFIIQRGDRVTLTKIEAPPTEWESPVNAFEHVYKHEQKVTGLINQLVNLALSLGDHATNNFLQWFVAEQVEEEESSSGVLKKVKMANDSLSGMLMLDNELSQRIFTPPATTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI5</accession>
    <name>F0TCI5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">egsA</name>
        <name evidence="15" type="ordered locus">Metbo_1015</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NAD(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>NADP(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644613.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1015"/>
    <dbReference type="EnsemblBacteria" id="ADZ09262">
        <property type="protein sequence ID" value="ADZ09262"/>
        <property type="gene ID" value="Metbo_1015"/>
    </dbReference>
    <dbReference type="GeneID" id="10277464"/>
    <dbReference type="KEGG" id="mel:Metbo_1015"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OMA" id="ALHGEQC"/>
    <dbReference type="OrthoDB" id="57545at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NADP+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 13">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ09262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="347" mass="36841" checksum="15E131DFE9FF2BFD" modified="2011-05-03" version="1">MDARKIQLPREIHTGAGMIKETGKICRNLRLDGDVMVVTGPNTRKIGGDAVIESLLEEDYNVNDITIDNPSKSSVEAVQDEIKQCSLVLGVGGGKVIDVAKLASTRAAVNFISVPTAASHDGIASPRASIKDEGGNVSLKAEPPIGVIADTEIISKAPFRLLAAGCGDIVSNYTAILDWKLAHRLLDIDYSDSAAALSEMTAKMTINSADAIKEGLTESAGLVVKALISSGIAISIAGTSRPASGSEHKFSHALDIVAPKPALHGEQCGVGTIMMMYLHGGDWKFIRDTLKVIKAPTTAYEMGIEPEYIIEALQMAHTIRSERYTILGDRGLTHESARVLAEKTGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI7</accession>
    <name>F0TCI7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0179 protein Metbo_1017</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644615.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1017"/>
    <dbReference type="EnsemblBacteria" id="ADZ09264">
        <property type="protein sequence ID" value="ADZ09264"/>
        <property type="gene ID" value="Metbo_1017"/>
    </dbReference>
    <dbReference type="GeneID" id="10277466"/>
    <dbReference type="KEGG" id="mel:Metbo_1017"/>
    <dbReference type="eggNOG" id="arCOG04477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121764_0_0_2"/>
    <dbReference type="OMA" id="CPSHEYC"/>
    <dbReference type="OrthoDB" id="108341at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="PTHR40699"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="16300" checksum="ABE00759967173A7" modified="2011-05-03" version="1">MITLIGKNLANRGLKFMFYGASSECESCRFKNTCIDTLESGRMYIIKQVKNGEQPCQIHEGGKVRVVEVEKAYIKCLVDSKKSFEGSKLVFETPSCDDQDCNMRSKCFPEGLMNQDKCKIIKNLGKPKHECPQGRELNNVLLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK3</accession>
    <name>F0TCK3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644631.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09280">
        <property type="protein sequence ID" value="ADZ09280"/>
        <property type="gene ID" value="Metbo_1033"/>
    </dbReference>
    <dbReference type="GeneID" id="10277482"/>
    <dbReference type="KEGG" id="mel:Metbo_1033"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_2_2"/>
    <dbReference type="OMA" id="ARITFFY"/>
    <dbReference type="OrthoDB" id="99962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16368" checksum="739590390DDA5F52" modified="2011-05-03" version="1">MKKILLPTDGSENSEKAGEYAISLADISGADILVLNVIDTYYLNALPQRDLRKSLEEELRADGKKAVENFKAKLEESQCEGYCKNIKFRVLIKEGKPSEVILKTIDEEGIDQVIMGKSGKHGIEEFLLGQTTERVVRESKVPVNVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL0</accession>
    <name>F0TCL0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1040"/>
    <dbReference type="EnsemblBacteria" id="ADZ09287">
        <property type="protein sequence ID" value="ADZ09287"/>
        <property type="gene ID" value="Metbo_1040"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1040"/>
    <dbReference type="eggNOG" id="arCOG01474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_2"/>
    <dbReference type="OMA" id="IEMHLIV"/>
    <dbReference type="OrthoDB" id="110591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="7">
        <location>
            <begin position="220"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="32729" checksum="EA4564E67760B883" modified="2011-05-03" version="1">MVMLLENQDDLRERIGRKASTVAIVGNIFLTIFNFVIGTLSGSSALIAESAHTLSDVLTSLIAFVGFKIGMKPADDDHQYGHGRAEPLVGLVIVVFLVFVAYEILSGVYIKLISGNALVAPDLIAAGMALVGILTNVVLTKYLLSAGEKIHSPAIIADGQHQKVDIFSCIAIFVGVLGSQLGYTFLDPLVAIIIALIVIKTAIQLGIDNVNILMGKVPSNDIIKEIESKSMKVSGVKGVHGIRINPMGPYCSVVLHIELSGELKLKEAHVIAHNVEHEILTNVSLIKIATVHVCPHEEECGTEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL9</accession>
    <name>F0TCL9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644647.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1049"/>
    <dbReference type="EnsemblBacteria" id="ADZ09296">
        <property type="protein sequence ID" value="ADZ09296"/>
        <property type="gene ID" value="Metbo_1049"/>
    </dbReference>
    <dbReference type="GeneID" id="25394723"/>
    <dbReference type="KEGG" id="mel:Metbo_1049"/>
    <dbReference type="eggNOG" id="arCOG02352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="66"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="139"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="320"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="390"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="605" mass="69847" checksum="EBC469BB3392AC3C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS0</accession>
    <name>F0TCS0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PK_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645811.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2246"/>
    <dbReference type="EnsemblBacteria" id="ADZ10460">
        <property type="protein sequence ID" value="ADZ10460"/>
        <property type="gene ID" value="Metbo_2246"/>
    </dbReference>
    <dbReference type="GeneID" id="10278712"/>
    <dbReference type="KEGG" id="mel:Metbo_2246"/>
    <dbReference type="eggNOG" id="arCOG04343">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095207_1_0_2"/>
    <dbReference type="OMA" id="VTYHQGF"/>
    <dbReference type="OrthoDB" id="104210at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015074">
        <property type="entry name" value="DUF1867"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016138">
        <property type="entry name" value="UCP016138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PK_C" evidence="1">
        <location>
            <begin position="18"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="189" mass="20313" checksum="1E8AB9CD45FBB7A5" modified="2011-05-03" version="1">MEKTIQYFERPGKENTDELIQLVKTRMEELGIKDIVVASASGSSAVKLVKALDNPELNIVNVTHHSGFKGEDIIELEPEYRTELEKNGVKTFIGSHSLSGVGRGISNKFGGITPVEIIAATLRLFSQGVKVGVEISIMTADAGLIPTDTEIIAVGGTGKGLDSAIVLKPAHMGNFFDLKINEIIAMPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV8</accession>
    <name>F0TCV8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645849.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2284"/>
    <dbReference type="EnsemblBacteria" id="ADZ10498">
        <property type="protein sequence ID" value="ADZ10498"/>
        <property type="gene ID" value="Metbo_2284"/>
    </dbReference>
    <dbReference type="GeneID" id="25394757"/>
    <dbReference type="KEGG" id="mel:Metbo_2284"/>
    <dbReference type="eggNOG" id="arCOG02353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_282645_0_0_2"/>
    <dbReference type="OMA" id="INRAFYD"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T6</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
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    </evidence>
    <sequence length="287" mass="32051" checksum="68F0555F992FA051" modified="2011-05-03" version="1">MKKIRFTDISVTVISHTFKQRFLLAKICNTLPPVAWVVKKLFFDGDDIQVLPMDKAVKKNSLVQSIDVDSKVPLSESSPVPSDVLRLMIKQSRYRFILNFCICRTANNCEKFPHDLGCLFLGKGASRISPSVGRPVSIDEAIAHIEKCGEAGLVHIIGRNKIDSIWLNAGKHDELLTICNCCDCCCLWKMATYLPEHIGSSLMPMDGIELVYNRDFCTLCGACTKDECFVHAITMDTDGVTIDKKRCRKCGRCVDTCRHGGLTISMDSNTVNRSLEHVEKLVDVNMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T8</accession>
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        <submittedName>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="93"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="47205" checksum="6656E1A64693C5D1" modified="2011-05-03" version="1">MPLAIIAFLVVMLVPLPGLSYPGHAAIALLIFAIIMWASEAQHLAVTSLILLFLQPILGIASFGNAVIGFANPILFLMIGGFILAVAIGKSGLAKRFTYWMLSKVGTTPNMSIFAAVFSTGLLSAWIENVVAFAMLLPIIKTIIPLFGINDAEKGNSNFAKAMVLGASYGSLAGGFGTEIGTAPNLMAAAYTHLPFANWMVFGFPLAIIMLIVTWKLLGWIFPPEVDGIVGGKETLHKSMSVMGPISKQEKITAVILFFTIGLWVTTGITGLDSYSVALIGAALYFISGVIDWKDAQEGVDWGLIIFFGGALSLGAALLNTGAAAWLIQDLIGLMGNSVSTLAIMLMLMIIAVIFTQVMSNIALAAILVPLSVTLAAAQGQPVGIYAVPVAISCSLSFMFPMADPTVAMAYGTGYVKIKEILKAGIPMVVIGIIITIAVILTIGKPFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V3</accession>
    <name>F0T5V3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644697.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1101"/>
    <dbReference type="EnsemblBacteria" id="ADZ09346">
        <property type="protein sequence ID" value="ADZ09346"/>
        <property type="gene ID" value="Metbo_1101"/>
    </dbReference>
    <dbReference type="GeneID" id="10277551"/>
    <dbReference type="KEGG" id="mel:Metbo_1101"/>
    <dbReference type="eggNOG" id="arCOG02589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_2"/>
    <dbReference type="OMA" id="ANEIVIC"/>
    <dbReference type="OrthoDB" id="103133at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="7"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17389" checksum="2068C4A0CF13DC36" modified="2011-05-03" version="1">MSLDETEILLVEDNPTDAELTMRALKKKNLINKLVWVKDGAEALDFIFAKGEFSDRNPEDLPKLILLDLRMPKVDGLEVLHKIKENENTKNIPVVVLTSSKEDRDIVESYKLGVNSYVSKPVEFDEFIEAVSTMGFYWILINKPPSEVPHGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y5</accession>
    <name>F0T5Y5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZnF_CHCC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1134"/>
    <dbReference type="EnsemblBacteria" id="ADZ09378">
        <property type="protein sequence ID" value="ADZ09378"/>
        <property type="gene ID" value="Metbo_1134"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1134"/>
    <dbReference type="eggNOG" id="arCOG14543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710991_0_0_2"/>
    <dbReference type="OrthoDB" id="133262at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZnF_CHCC" evidence="4">
        <location>
            <begin position="301"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="360" mass="42615" checksum="FE05617A2A9CBC98" modified="2011-05-03" version="1">MKGILSFYESFYGSNFYRHFRRPPDFNKSNFRIQFTVKDPKSLFVHVHRNNGNHPCLIHTYDHGTIDNLKEKKNSIMVFDRVFLDFDVRNEEARKIKNELVKLRSSGLNYKKSLQNSLQEKLQNLVINEKISKPAVNDAKDFAIKIEETFEKPPILFFSGFKGCHAYIFFKPTEFKDINLAVLWFAKHVKKSYGYSTLDLSVTRDAKARLSRVPYSKHQLTDLIVVPFQLSDDYEDIMARSLDPSVENFDIEDYCTNFSEHLQEIDEIEFYNSKIRKTTQKSEMATKNSFDDVTDQLILFKQILGTPVRVYPEKEYVMYHCPFHDHEDKKPSFRVNKKGYYCYGCGRKGNYWDFLKKNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z6</accession>
    <name>F0T5Z6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative circadian clock protein, KaiC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644740.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1145"/>
    <dbReference type="EnsemblBacteria" id="ADZ09389">
        <property type="protein sequence ID" value="ADZ09389"/>
        <property type="gene ID" value="Metbo_1145"/>
    </dbReference>
    <dbReference type="GeneID" id="10277595"/>
    <dbReference type="KEGG" id="mel:Metbo_1145"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OMA" id="GKMERYL"/>
    <dbReference type="OrthoDB" id="76011at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="25643" checksum="905422E7AB1313CB" modified="2011-05-03" version="1">MKRVKSGIEGIDNITGGFPQGRSMLVTGDAGSGKTIFGLQFAKSSCAQGFKTTYITTEEDAKDLYTQGESFNWDMKSQTETGKLNFVELAGVRAKVTEAEMSIDIGAMKGNFTKFVNEIPEDTNTVVIDNIGSYTAKLTPYEFRDRFDLLVYELKKRDITALIILDSATSKEFNEIALFSVYGAMRLMKRENPYTGRRERVMDIVKMRSTKTPTQFISYEINSDGISMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T623</accession>
    <name>F0T623_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10530">
        <property type="protein sequence ID" value="ADZ10530"/>
        <property type="gene ID" value="Metbo_2316"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2316"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_623509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="30"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="439" mass="49452" checksum="F732C5116E54743D" modified="2011-05-03" version="1" precursor="true">MNGGIVRTRFMLVILIFILFSVLGTVSAKDVSKPAVVSMDPVNNSCILSGSPVLKVVFSENIKLGTDYVELRNSNKKDIPINKSLNNSILTIKPNTILTNGSKYTLYLHSGSYTDFAGNSMNIYSTCFTVKTAPSYQIYLEKIQTDMKIKGKSVYAEYAIYQKPTNSTTWNRYLFNHIVNKAKTGVNGPENDYRISKLQNVVLNDNTKNLQIKSSTDLTSPGNWEKAVLLTGKSEKIGGMHGYENYTEIVFYKDGKLLFPNINNPITKCKNLQIKETSNLFNPNNPSQIVAKTITTYEWNGEALKIQNIYNWKINTTVLEAYVAMFPTKNSPTVTSEGKINGLLTENFQTRTTDLHGNSAIAVVWNNLNNLHMSMEITNPIIAFNNYSNTGNTTHGETWFRTASSYIKLYMSRVCQPKTEKITPNTTWNIQTTYKVWNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T646</accession>
    <name>F0T646_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PyrE-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645904.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2340"/>
    <dbReference type="EnsemblBacteria" id="ADZ10553">
        <property type="protein sequence ID" value="ADZ10553"/>
        <property type="gene ID" value="Metbo_2340"/>
    </dbReference>
    <dbReference type="GeneID" id="10278806"/>
    <dbReference type="KEGG" id="mel:Metbo_2340"/>
    <dbReference type="eggNOG" id="arCOG00028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111001_0_0_2"/>
    <dbReference type="OMA" id="DIHVDWS"/>
    <dbReference type="OrthoDB" id="77963at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01214">
        <property type="entry name" value="PyrE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022854">
        <property type="entry name" value="PyrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF41">
        <property type="entry name" value="PTHR19278:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="3">
        <location>
            <begin position="78"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="21609" checksum="36B45A05B9B8D5F7" modified="2011-05-03" version="1">MNEKLIKRAYELRNRGFTTGEIADELNVSKDTSRWLILQGKDNKTSAESEKAPIDFAINWNSLGGSSVRMKYVSAALADMALKYGEPDVIVGITVSGIPFATMMAEILGADISVFHPIKHRKMEEDAKGAVSSNFASVDGRKVVIVDDVTTSGRTIREAARVYKDLGAIPIAVVVLIDKAGIAEVENVPVESLISVSRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T682</accession>
    <name>F0T682_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644748.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1153"/>
    <dbReference type="EnsemblBacteria" id="ADZ09397">
        <property type="protein sequence ID" value="ADZ09397"/>
        <property type="gene ID" value="Metbo_1153"/>
    </dbReference>
    <dbReference type="GeneID" id="10277603"/>
    <dbReference type="KEGG" id="mel:Metbo_1153"/>
    <dbReference type="eggNOG" id="arCOG10249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_398830_0_0_2"/>
    <dbReference type="OMA" id="TVWNFHN"/>
    <dbReference type="OrthoDB" id="3021at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="682" mass="73336" checksum="140040F56C05934F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T687</accession>
    <name>F0T687_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Universal metal-binding-domain/4Fe-4S-binding-domain containing ABC transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644753.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1158"/>
    <dbReference type="EnsemblBacteria" id="ADZ09402">
        <property type="protein sequence ID" value="ADZ09402"/>
        <property type="gene ID" value="Metbo_1158"/>
    </dbReference>
    <dbReference type="GeneID" id="10277608"/>
    <dbReference type="KEGG" id="mel:Metbo_1158"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OMA" id="NYALYWA"/>
    <dbReference type="OrthoDB" id="101215at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005237">
        <property type="entry name" value="MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="PTHR12196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00289">
        <property type="entry name" value="TIGR00289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthami_syn_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="25314" checksum="ADEE1F07B2CFA5A0" modified="2011-05-03" version="1">MKAAVLYSGGKDSTMAVYKAIEEGYNVEYLVSMISDNPESYMFHVPNIHITELSAEAMGIPLLRAKTHGIKEKELDDLKKVLLDLKARGVDAVFAGALASQYQKSRIDGLCEDIGLESKAPLWHWDPEDYMETIIELGFEVILISVSAEGLDENWLGRKLDQELLKEIVDLHGKYGMHMAFEGGEAETLVLDGPIFNKRIKIQETSNVWETDSGYMVISKAELEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D8</accession>
    <name>F0T6D8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="10">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cysS</name>
        <name evidence="12" type="ordered locus">Metbo_1210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644804.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1210"/>
    <dbReference type="EnsemblBacteria" id="ADZ09453">
        <property type="protein sequence ID" value="ADZ09453"/>
        <property type="gene ID" value="Metbo_1210"/>
    </dbReference>
    <dbReference type="GeneID" id="10277660"/>
    <dbReference type="KEGG" id="mel:Metbo_1210"/>
    <dbReference type="eggNOG" id="arCOG00486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_2"/>
    <dbReference type="OMA" id="AKYWMHN"/>
    <dbReference type="OrthoDB" id="20883at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="11">
        <location>
            <begin position="357"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="269"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="467" mass="54576" checksum="72F44800E4E2F428" modified="2011-05-03" version="1">MIKLYNTMTRRKEIFEPIEGNRVKLFVCGPTVYDYSHIGHARTYISFDVIVRYMKYSGYSVFYVQNITDLDDKILKRADELETSPLELARRYEEKYLRDMELLGVEHVNLYARATEHIPEIVRQIGVLVDKGYAYETDKGVYFDESKFEDFGKLSNRNLEDLNVHRIGPDSSKRNPGDFALWKKRERISEEQEMIWDSPWGTGRPGWHIEDTAITETYFGPQYDIHGGGLDLIFPHHEAEIAQMEASSGIKPMVRYWMHTGFLNVKGEKMSKSLGNFITINELLEEYSPEVFRFFVLSTHYRSPIDFSSEILHQSSQGLNRIYKLIEKINSELDSDTNISLDNDSDYIQKLAAVREEFFEAMDNDFNTPAAISTIFDLIRDVNRDIHESNISKNSLTKLKAIIIEFGYVLGLDFSMEPENEENLEVELLDLLKYVREKLRQNKNWELSDQIRDRLNELNITLEDKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G5</accession>
    <name>F0T6G5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ku domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645950.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2386"/>
    <dbReference type="EnsemblBacteria" id="ADZ10599">
        <property type="protein sequence ID" value="ADZ10599"/>
        <property type="gene ID" value="Metbo_2386"/>
    </dbReference>
    <dbReference type="GeneID" id="10278852"/>
    <dbReference type="KEGG" id="mel:Metbo_2386"/>
    <dbReference type="eggNOG" id="arCOG10146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048975_1_1_2"/>
    <dbReference type="OMA" id="IRYRKVC"/>
    <dbReference type="OrthoDB" id="87420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="Ku70/Ku80_beta-barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009187">
        <property type="entry name" value="Prok_Ku"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41251">
        <property type="entry name" value="PTHR41251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006493">
        <property type="entry name" value="Prok_Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SSF100939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ku" evidence="2">
        <location>
            <begin position="52"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="28989" checksum="DCDDB427C33B6646" modified="2011-05-03" version="1">MRSIWSGYLSFGTILVPIRSYAASGNLHISFHQVHKEDCGRVRYKKVCEKDGEELKPEDITKAYIVGGECLQFTDEELNTLKPFSTKIMEIKGFCEMDEIPLEALSKPYYLGTDAPTKGGAGKSFLLLKEAMKRSKKVAIVKWVSRTNEYIGMIMPHGKGLLLKQLLYNEQVRSIDEVEVLESSVTDELVDKGVQAINKMTFSFKWADYTETYTKEVKELIEKRFLGEEVEAEEFSLPETRSIEAELEKMLETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H9</accession>
    <name>F0T6H9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6 9">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1 6 9">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pgk</name>
        <name evidence="10" type="ordered locus">Metbo_2400</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1 6 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645964.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2400"/>
    <dbReference type="EnsemblBacteria" id="ADZ10613">
        <property type="protein sequence ID" value="ADZ10613"/>
        <property type="gene ID" value="Metbo_2400"/>
    </dbReference>
    <dbReference type="GeneID" id="10278872"/>
    <dbReference type="KEGG" id="mel:Metbo_2400"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="46017at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6 7">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6 7">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <begin position="359"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="44653" checksum="FE0DDDE4BEE957FF" modified="2011-05-03" version="1">MSLDFYTIDDFDLDDKTVLVRVDINSPVDPSSGLILDDTRIKLHAETIAELSKKGAKTVVLAHQSRPGKKDFTTLEQHADALSNILKHEVAYVDDIFGSHARNAIGKMKRGDILLLENVRFYSEEILKRDPKLQSETHMVQNLAPMADYFVNDAFAAAHRSQPSLVGFAFALPSAAGRIMERELKALYNAMNNVQKPCIYVLGGVKVDDSIMVMENVLENGSADYILTTGLVANIFLWGSGVNIRKYNKEFIEKKGYCDFVRKSKKLLKNFGDQIILPKDLAVCMGDKRVEFSVEDLPNQPIFDIGTETIKEYAGYIRRANTLFANGPAGVFEKDDFNIGTDDILNAIASSPGFSIIGGGHLAAAANQMGLSKGISHISSGGGASINLLAGEDLPVVTVLRDHAAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L7</accession>
    <name>F0T6L7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIM-barrel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646002.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2438"/>
    <dbReference type="EnsemblBacteria" id="ADZ10651">
        <property type="protein sequence ID" value="ADZ10651"/>
        <property type="gene ID" value="Metbo_2438"/>
    </dbReference>
    <dbReference type="GeneID" id="10278910"/>
    <dbReference type="KEGG" id="mel:Metbo_2438"/>
    <dbReference type="eggNOG" id="arCOG00605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118194_0_0_2"/>
    <dbReference type="OMA" id="ELNCHCR"/>
    <dbReference type="OrthoDB" id="72918at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005270">
        <property type="entry name" value="tRNA_dU_NifR3-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00736">
        <property type="entry name" value="nifR3_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dus" evidence="1">
        <location>
            <begin position="75"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="238" mass="25978" checksum="3F652535CCAF745B" modified="2011-05-03" version="1">MAGVTDGKFCRGMIDYGFDLLTLGGYNAEKASINAGLKIIQRGRKEFHVEEPDLVNHITSEAKYIKDGSNKNVGVSVNLRAITPDPIIKISKIPEVDVVEINAHCRQAELVKSGYGQALMNYPDELYNLVHGTVEGCSKQVSVKIRANVEGVNYLKVLEAIENAGAHYIHVDAMKPGYDSADYDFITWVKRNTDSFVIGNNSIKDIASARKMLSSGADGISIARAAIRGNIPFDMSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M8</accession>
    <name>F0T6M8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643612.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0008"/>
    <dbReference type="EnsemblBacteria" id="ADZ08261">
        <property type="protein sequence ID" value="ADZ08261"/>
        <property type="gene ID" value="Metbo_0008"/>
    </dbReference>
    <dbReference type="GeneID" id="10276430"/>
    <dbReference type="KEGG" id="mel:Metbo_0008"/>
    <dbReference type="eggNOG" id="arCOG00696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_2_2"/>
    <dbReference type="OMA" id="HCIDNGV"/>
    <dbReference type="OrthoDB" id="44408at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="1">
        <location>
            <begin position="57"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="45279" checksum="657FDEF677E2275D" modified="2011-05-03" version="1">MSYTLIRNGTLINGNGGKPIKNAAVLIKNNIIIAAGSEKAIEIPKTDLKIIDAEECFILPGFIDAHVHIMTEGFAREDTIYTPHSLYFYNAIEFMKKTIFAGITTARDAGMADVGVKMAIDKGLITGPRLQISVAPLTITGGHFDFWLTSGFDIKPMYPGLPDGIADGPENVRKKVREVMRSGAEFIKVMVTGGVISANDKPEHPQFTLEELKVMVEEAKFRDLQVVAHAHGNKGIINAVTAGVNSVEHGTCLDDESIGLMLENNTYLVPTFIAMKINKEMAEDETSSIPDWSREDAIRMEKVHETNMRRAYQAGVKIVMGTDSGVVPHGQNLKELGYLCDMGMDPMEAIVAGTKRAAESLRLDSKVGTLEAGKLADVVLSKKNPLEYIKTLGNPDNIVMVIKDGVIVKNIHSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S1</accession>
    <name>F0T6S1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine dehydrogenase</fullName>
            <shortName evidence="1">AlaDH</shortName>
            <ecNumber evidence="1">1.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ala</name>
        <name evidence="2" type="ordered locus">Metbo_0051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(+)-dependent oxidative deamination of L-alanine to pyruvate, and the reverse reaction, the reductive amination of pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-alanine + NAD(+) = H(+) + NADH + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18405"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="1.4.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family. Archaeal alanine dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643655.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0051"/>
    <dbReference type="EnsemblBacteria" id="ADZ08304">
        <property type="protein sequence ID" value="ADZ08304"/>
        <property type="gene ID" value="Metbo_0051"/>
    </dbReference>
    <dbReference type="GeneID" id="10276474"/>
    <dbReference type="KEGG" id="mel:Metbo_0051"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_3_1_2"/>
    <dbReference type="OMA" id="AVKAFTY"/>
    <dbReference type="OrthoDB" id="96993at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000286">
        <property type="term" value="F:alanine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006522">
        <property type="term" value="P:alanine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00935">
        <property type="entry name" value="AlaDH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012742">
        <property type="entry name" value="Ala_DH_archaeglobus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028609">
        <property type="entry name" value="AlaDH_arch-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02371">
        <property type="entry name" value="ala_DH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="335" mass="37132" checksum="2431910B0BEA9518" modified="2011-05-03" version="1">MKDATRYETLLLKQSEIKELIEMKEIIESVETAYTVHATRNVQMPAKKYLFFKKYNGDLRIMPAFIKNMDEAGVKCVNVHPENPVKYNLPTVMGIIQLFDPKSGFPLSVMDGTWITNMRTGAAAGVGTKYLAREDSKTLGIIGAGKQAFTQLMALKEVMDIEHAHVFCRTCSSRENFADMARKRFDIDINAVSTAEEAVKNMDVVVTVTPANKPVIKTEWITEGTHINAMGADAPGKQELEVGILQKARIFIDCWEQARHSGEINVPVHDGIITRKSISSKIGDVIIGNAEGRISDEDITVFDSTGLAVQDMVTGWKVYEEAVKKGVGTVINFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T5</accession>
    <name>F0T6T5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643669.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0065"/>
    <dbReference type="EnsemblBacteria" id="ADZ08318">
        <property type="protein sequence ID" value="ADZ08318"/>
        <property type="gene ID" value="Metbo_0065"/>
    </dbReference>
    <dbReference type="GeneID" id="10276488"/>
    <dbReference type="KEGG" id="mel:Metbo_0065"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_0_2"/>
    <dbReference type="OMA" id="FINPFAE"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="136"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="247" mass="27469" checksum="C9735DB9D1E159A2" modified="2011-05-03" version="1">MGPNILVVEDERITAEDIKSGLECSGYNVIDMVASGKEAIESAGKLQPDLVLMDIKLKGKMDGIEAAGQIKLKYDIPVIYLTAFSDEYTLTRAKATEHSGFVMKDETGLIKKPFEESELHSAIATTLYRHKMEKNHDNLVSAMLKHYNEAVIATNSEGLVKLMNSSAENLTGFKLDKVVGKKVSTVFQPLKTFESFDLTLMKEHLQQNEHILVSNGQEIKVKCDLNSISDNDGQINGFILVFKQKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W4</accession>
    <name>F0T6W4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1242</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ09484">
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        <property type="gene ID" value="Metbo_1242"/>
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    <dbReference type="KEGG" id="mel:Metbo_1242"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
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        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
            <begin position="180"/>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="448"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <sequence length="458" mass="49147" checksum="C6254C54DC005E84" modified="2011-05-03" version="1">MDLKKILLVKKDIKDCTTCDSGLSQTFGSIGLIILGVGAILGAGIFVVTGLAAAVAGPSIIFSFSAAAFVCVMVALCFAEMASIITVTGGSYTYTQFSLGEIWAWIVGWSVVLQIIITGATVAIGWSSYFTGLMVSLNINFPNLISQYTTMINLPALIIVALLTGVVLMGARSSKRVNNVIVIIKLAVVIIFVLMGIKYVNTANYIPFMPNGINSVFQGAAMVFFAYLGFEAVATSSEEAINPQKSIPKGIIGSLVLCAVLYILVVIIMTGMVKYSEYSGVAAPVQYALNAVGANWIMTIVTIGIMAGLTTVILVNLYIIPRVIYSMSRDGLLPSKLSHINTKYKVPIIPIIISGVLIGIIASFVPLGSVFELANIAALTAFIFVAIGIINLRRTCPDVPRKFKCPLVPVLPLITIVICLVLILELKLSTILYFSIWTVLGLTIYFGYKCYKLKKVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y6</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">AL-21</strain>
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    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="27"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="157" mass="16846" checksum="55AB78BD8E2312F1" modified="2011-05-03" version="1">MSKASSSDIKGKIIAICTSSVKGTKKKDIFQGILMENYGLLGDGHAENQSHRQLSLLALESIQKMRDLGLEVNPGDFAENVTTEGVELAKLPIGTRIALGNNSIVEVTQIGKECHTQCEIGKQTGQCIMPTEGIFCRVLEGGKIKAGDSLKVFKLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y9</accession>
    <name>F0T6Y9_METLA</name>
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        <submittedName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7233" checksum="A6905534B1C9BA99" modified="2011-05-03" version="1">MKIIFSEGQTENLNIENVSVKELLEDLEIEPLEVIVKKGSLVLQEDETIQNNDEIMIIKVIHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A9</accession>
    <name>F0T7A9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643689.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0085"/>
    <dbReference type="EnsemblBacteria" id="ADZ08338">
        <property type="protein sequence ID" value="ADZ08338"/>
        <property type="gene ID" value="Metbo_0085"/>
    </dbReference>
    <dbReference type="GeneID" id="10276508"/>
    <dbReference type="KEGG" id="mel:Metbo_0085"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_4_2"/>
    <dbReference type="OMA" id="VQCEYFA"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="28013" checksum="4610307E71F9FA51" modified="2011-05-03" version="1">MAEIIAILNQKGGCGKTTTAVNLAAALALNDKRVLVVDMDPQGNATTGFGIQKNEVDSTIYSVLTGNSSVEDATVSTDISGLDVVPSNIALSGAEIELSKEVGYHTILELAMESVAENYDYIFIDVPPSLGILTINCLVAANSVIIPIQAEFYALEGMADLLEAIGLVERRLKSPSPIKGILLTLYDSRTRLGRDVYSNVKEYFGQSEYVFKTTIPRNVTLAEAPSHGKPCIIYDEESSGSQAYVDLAKEIIKIDGTVDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B6</accession>
    <name>F0T7B6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_0092</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643696.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0092"/>
    <dbReference type="EnsemblBacteria" id="ADZ08345">
        <property type="protein sequence ID" value="ADZ08345"/>
        <property type="gene ID" value="Metbo_0092"/>
    </dbReference>
    <dbReference type="GeneID" id="10276515"/>
    <dbReference type="KEGG" id="mel:Metbo_0092"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OMA" id="CFGTSGP"/>
    <dbReference type="OrthoDB" id="7134at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="190"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="383"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ08345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="577" mass="62466" checksum="6A22FC62A8D5B99B" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L2</accession>
    <name>F0T7L2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644931.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1339"/>
    <dbReference type="EnsemblBacteria" id="ADZ09580">
        <property type="protein sequence ID" value="ADZ09580"/>
        <property type="gene ID" value="Metbo_1339"/>
    </dbReference>
    <dbReference type="GeneID" id="10277790"/>
    <dbReference type="KEGG" id="mel:Metbo_1339"/>
    <dbReference type="eggNOG" id="arCOG03045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901979_0_0_2"/>
    <dbReference type="OMA" id="NIATIYF"/>
    <dbReference type="OrthoDB" id="72935at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="308" mass="35099" checksum="4D4C0DA759C30140" modified="2011-05-03" version="1">MMEFFLVNGFFDRFAGLEKENDPGDESEEYDLDEYLDILNEYRDAEADILINIATIYFENENMEESLKYLEEAIIIYDGLDYLEKKALVMDIMGDIYYNTDEKRTALEYYKEAFKLYSETDSDTKENIISKINETEKDLVSVQSKSTLSSVKPQKKAPLEVSTDDYIGISTEIEEVIGILNGAKTYMSYANHENPIKQLENAYEMSSGIGDNDASATILMIKGNLTLKESKPVEALEDFKKGLELFKLTNNFTGEAVAMLLIGTAHYITGNMDIVAENFRKSIELLRQINNISGEEKAIKLMNTIYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M8</accession>
    <name>F0T7M8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase S26B, signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1356"/>
    <dbReference type="MEROPS" id="S26.017"/>
    <dbReference type="EnsemblBacteria" id="ADZ09596">
        <property type="protein sequence ID" value="ADZ09596"/>
        <property type="gene ID" value="Metbo_1356"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1356"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_4_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="4">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16067" checksum="6311C708ACB26E15" modified="2011-05-03" version="1">MLILISIFAVGIVLAQHLNVVSSESMEPLLHKGDIVIINYETDNIDVGDVVVYNATWFDHKPVIHRVINKQAVNGSYIYTLKGDNNQKEDPEIVYPKAVIAKVVKINHQMLILPKLGYISFWFHELMEYFFGQTILYYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N2</accession>
    <name>F0T7N2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-dehydroquinate synthase</fullName>
            <shortName evidence="3">DHQ synthase</shortName>
            <ecNumber evidence="3">1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroB'</name>
        <name evidence="4" type="ordered locus">Metbo_1360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + H2O + NAD(+) = 3-dehydroquinate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644951.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1360"/>
    <dbReference type="EnsemblBacteria" id="ADZ09600">
        <property type="protein sequence ID" value="ADZ09600"/>
        <property type="gene ID" value="Metbo_1360"/>
    </dbReference>
    <dbReference type="GeneID" id="10277811"/>
    <dbReference type="KEGG" id="mel:Metbo_1360"/>
    <dbReference type="eggNOG" id="arCOG04353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056379_0_0_2"/>
    <dbReference type="OMA" id="HFGMAIK"/>
    <dbReference type="OrthoDB" id="29853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQ_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="entry name" value="PTHR33563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="374" mass="40842" checksum="5FA16C4113332CD1" modified="2011-05-03" version="1">MKFAWIKAEGQNWDEKKLLITAALESGIDHIVDYEDPEKIAKLGNLSVVSNTKDSDIHMVGINGEGDGTLTIPDDLSNSKDLADLTALKASGSTVAAYMEINSKKHEELASKLGKTADYLVLQGKDWTVIPLENIIAELQGQDVKIIAAVKDFDEAKLALETLEYGTDGVLLIPNDISQIKKVAEFIEKIDSETYQLVAANVTKVEPVGSGDRVCVDTCSMMQVGEGMLVGSYSKGLFLVHSESLESEYVASRPFRVNAGPVHAYVMTPGNKTRYLSEIETGDEVLTVDSKGNTKTTVVGRVKIEKRPLMLVEAEHEGVSVRTLLQNAETIRLINQDGDPVSVAQLKVGDRVMVYLDKSARHFGMAIEESIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P1</accession>
    <name>F0T7P1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclic 2,3-diphosphoglycerate synthetase</fullName>
            <shortName evidence="5">cDPGS</shortName>
            <ecNumber evidence="5">6.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpgS</name>
        <name evidence="6" type="ordered locus">Metbo_1369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2R)-2,3-bisphosphoglycerate + ATP + H(+) = ADP + cyclic (2R)-2,3-bisphosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:79081"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.5.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclic 2,3-diphosphoglycerate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.9" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644960.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1369"/>
    <dbReference type="EnsemblBacteria" id="ADZ09609">
        <property type="protein sequence ID" value="ADZ09609"/>
        <property type="gene ID" value="Metbo_1369"/>
    </dbReference>
    <dbReference type="GeneID" id="10277820"/>
    <dbReference type="KEGG" id="mel:Metbo_1369"/>
    <dbReference type="eggNOG" id="arCOG01230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_638764_0_0_2"/>
    <dbReference type="OMA" id="RRCGGGM"/>
    <dbReference type="OrthoDB" id="18931at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036356">
        <property type="term" value="F:cyclic 2,3-diphosphoglycerate synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01908">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016557">
        <property type="entry name" value="Cyc_diphosphoglycerate_synth"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009445">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="459" mass="50771" checksum="CBF954089968F0FB" modified="2011-05-03" version="1">MKTLRRMLCLVDGEHYFPVTKSALDMLDSLEHNEVVAAVFIGGTEKLRDASEDGISKQLERPVHFGPDHHNIPYELIDELIVRYNVDVVMDLSDEPVVDYSKRFKIANIVLSQGIPYEGPDFNFEPVTEHEVLKKPSLKILGTGKRIGKTAVSAYAARLIHKNEYNPCIVAMGRGGPEVPEIVHGDKIEITPEYLMEQSDKGVHAASDHWEDALMSRILTIGCRRCGGGMVGEVFITNMKRGAELANDVDSNFVIMEGSGAAIPPIKTDKQVVLVGANQPIVNIENYFGPYRIKLADLVVITMCEEPMASTEKVETIKNFIQEINPEATVIATVFRPKPLGDVKGKNVLFATTAPDSIKSVLIEHLEDFYGCKVVGTTPYLSNRPLLQKDIEKYIDEADVMLTELKAAAVDVATKDALKAGLEVIYCDNIPIDIEDGNNKNFDKAIIEVVDNAIKSFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P6</accession>
    <name>F0T7P6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09614">
        <property type="protein sequence ID" value="ADZ09614"/>
        <property type="gene ID" value="Metbo_1374"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1374"/>
    <dbReference type="HOGENOM" id="CLU_1763885_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="17238" checksum="5E1C1A5060C39517" modified="2011-05-03" version="1" precursor="true">MDMKKIMPLFSIIMVVGIITMGVQPVVAHTSEIKFLNNDYSVKTDDYVLNKTAEKYNEYNIRAQLYVNGEWQGYRFIYFELLDSNGTCLLSQSGMLTQPFMNTGTYWQIWYYEWKNLQPGTYTVKASYTGYLWYPSTEKTIKLHVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S1</accession>
    <name>F0T7S1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643759.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0156"/>
    <dbReference type="EnsemblBacteria" id="ADZ08408">
        <property type="protein sequence ID" value="ADZ08408"/>
        <property type="gene ID" value="Metbo_0156"/>
    </dbReference>
    <dbReference type="GeneID" id="10276582"/>
    <dbReference type="KEGG" id="mel:Metbo_0156"/>
    <dbReference type="eggNOG" id="arCOG03113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_4_2_2"/>
    <dbReference type="OMA" id="FMLQSID"/>
    <dbReference type="OrthoDB" id="127880at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="14074" checksum="D5314CE073463C7A" modified="2011-05-03" version="1">MKEILIVNLTKGTKIGKVEVADSFFSRFMGLMLRKKVGKGMLLKLPQSRSRHGSAIHMFFMRFPLDIVFADSDRKVVDMVTVDPWKTYTPRAPAKYVIELEKGSIKKFDLELGDDLDFTLECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X0</accession>
    <name>F0T7X0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="3">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pcn</name>
        <name evidence="8" type="ordered locus">Metbo_0205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643808.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0205"/>
    <dbReference type="EnsemblBacteria" id="ADZ08457">
        <property type="protein sequence ID" value="ADZ08457"/>
        <property type="gene ID" value="Metbo_0205"/>
    </dbReference>
    <dbReference type="GeneID" id="10276631"/>
    <dbReference type="KEGG" id="mel:Metbo_0205"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="OMA" id="EMKLINM"/>
    <dbReference type="OrthoDB" id="70433at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PTHR11352:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCNA_N" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PCNA_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="244" mass="27377" checksum="469820078D978026" modified="2011-05-03" version="1">MFKAVLSDSNVLKTSFEAISSIVDEVQMKADGDGLRLDALDRSHITFVHLELKSGLFDIYTCEEPLKINVDTEELMKVLKRAKSDDLVELTVDEGNLILTFEDEARRTFKIRLIDIEYEAPSPPDLDYPSEFEIPFSLLKNSIQDIGIVSDKISLMVDTDKFIASAEGEFGDAKIEYLHGEKINENAKSVFSLEKIKEMLKADKFSETAVLSLGNDMPLNLAMKMVSGDGELKFLLAPRIESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T834</accession>
    <name>F0T834_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645011.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09660">
        <property type="protein sequence ID" value="ADZ09660"/>
        <property type="gene ID" value="Metbo_1421"/>
    </dbReference>
    <dbReference type="GeneID" id="10277872"/>
    <dbReference type="KEGG" id="mel:Metbo_1421"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507404_0_0_2"/>
    <dbReference type="OMA" id="NESTYNT"/>
    <dbReference type="OrthoDB" id="131892at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="176" mass="18662" checksum="BC508DF75C81781E" modified="2011-05-03" version="1" precursor="true">MKKYIFVVIAIVALVVAASGCTSQNGNGNNSTTTKNYSNNSISFSYPANWDIISENSSENGTVVAVGDADIQKNNTTTGNGVVIFKLPNNANNTADLNTLKTQVASLNGTNSTVNIGGITANVTTFNTTSDNVTGQIRLIDFTKGDSLYFIQYTTLSSDFQTQGGLFDIITKSFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T859</accession>
    <name>F0T859_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1448</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645036.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09685">
        <property type="protein sequence ID" value="ADZ09685"/>
        <property type="gene ID" value="Metbo_1448"/>
    </dbReference>
    <dbReference type="GeneID" id="10277899"/>
    <dbReference type="KEGG" id="mel:Metbo_1448"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_1_0_2"/>
    <dbReference type="OMA" id="MYHHIIN"/>
    <dbReference type="OrthoDB" id="77132at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="28364" checksum="00CBF2299ADB9FDC" modified="2011-05-03" version="1">MIDYTLIFLPFVGFLIGLLVTTLGGGGGGLYVPILTLLGVAPQVAVATSLATVLPTTAVGAYSHNRKGNVDVKTGIILGIGGIIGTLIGAYVANLIPPNLLKKGLGLLLLIFAIPMIRRFIGVYKKSKTPKEEHHEIEENLSEDPDRETLKLTGPKRIFASLFGVAGGVLAGVFGLSGTPPISAGLYSLGLPTLMVVGTTVFVLVFNSLAGIGGYLFLGRFDVVLTVLLGGGAVVGSFLGPKLLEKAGKELVEKFIPPVLIGISVIFGLALIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T863</accession>
    <name>F0T863_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1801 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645040.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1452"/>
    <dbReference type="EnsemblBacteria" id="ADZ09689">
        <property type="protein sequence ID" value="ADZ09689"/>
        <property type="gene ID" value="Metbo_1452"/>
    </dbReference>
    <dbReference type="GeneID" id="10277903"/>
    <dbReference type="KEGG" id="mel:Metbo_1452"/>
    <dbReference type="eggNOG" id="arCOG09457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128703_1_0_2"/>
    <dbReference type="OMA" id="PKCGREF"/>
    <dbReference type="OrthoDB" id="97210at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014922">
        <property type="entry name" value="DUF1801"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08818">
        <property type="entry name" value="DUF1801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1801" evidence="1">
        <location>
            <begin position="23"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="14408" checksum="982798E62A3FA61B" modified="2011-05-03" version="1">MKKNKEQIKTFESYRKNYPIEIQKRLDQIQEAIKSAVPEAEEKISYNMPSYRFHGRLIYYGAFKNHIGFYPASGAVFKKFKDELKVYKQSGKGTVQFPYDKPLPIELIKKIAAYRAKENIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T869</accession>
    <name>F0T869_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dnaK</name>
        <name evidence="6" type="ordered locus">Metbo_0229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643832.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0229"/>
    <dbReference type="EnsemblBacteria" id="ADZ08481">
        <property type="protein sequence ID" value="ADZ08481"/>
        <property type="gene ID" value="Metbo_0229"/>
    </dbReference>
    <dbReference type="GeneID" id="10276659"/>
    <dbReference type="KEGG" id="mel:Metbo_0229"/>
    <dbReference type="eggNOG" id="arCOG03060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="entry name" value="Hsp_70_fam"/>
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        <source>
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    <sequence length="622" mass="66817" checksum="F5768F85B0830960" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A9</accession>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="60"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08521.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="82" mass="8859" checksum="A80245BA67976F72" modified="2011-05-03" version="1">MVYIGPLVLGFVIGFILGTRIKPNPESKMYFPASVYVLLAIVVIVMAYQLGPFPYYTDSILANGLISAFVGIVVGKLTFGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1488"/>
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        <property type="protein sequence ID" value="ADZ09721"/>
        <property type="gene ID" value="Metbo_1488"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_1488"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
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        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
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        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="371"/>
        </location>
    </feature>
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        <location>
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            <end position="235"/>
        </location>
    </feature>
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            <begin position="286"/>
            <end position="381"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="42126" checksum="BDB8F03F7FFF9CC5" modified="2011-05-03" version="1" precursor="true">MSFLSFAAKNPFRTKLRTIFIVILLVLGVIAVTASVGVSVYSTKLFGEMFNSGGADLSVLSDGYLSTGDLNNLTGIKDVQSAVGVSGYVISDNQNNTYNLNGFYGSNLLNNTDIPGIGHINLISGDWDNSSTGIILTEKTANRTNKKVGDTFYVSTLSSNQISNMNGTNPTVQVTNRTLNSDFKVVGIIKDTGNIDGIVQIQEANNLLFNSTDLKFNSIYLKTNNGKLDEVETSINSTYPQYHVVVGDALINSIKSFLFYLTSFFIGLGAIIMMIATLKSVSERTREIGVLKAIGWSNRRVMGMIMVESVVQLILAWIAAAVIMAILLVVLAPQFNVNIADLIRDNLSVALYTVLLSFGASLLMPVLGCLIPMIRVLRLNPTEALKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I9</accession>
    <name>F0T8I9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Archaeal Lon protease</fullName>
            <ecNumber evidence="13">3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metbo_1509</name>
    </gene>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645091.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1509"/>
    <dbReference type="MEROPS" id="S16.005"/>
    <dbReference type="EnsemblBacteria" id="ADZ09740">
        <property type="protein sequence ID" value="ADZ09740"/>
        <property type="gene ID" value="Metbo_1509"/>
    </dbReference>
    <dbReference type="GeneID" id="10277960"/>
    <dbReference type="KEGG" id="mel:Metbo_1509"/>
    <dbReference type="eggNOG" id="arCOG02160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="OMA" id="IPCSHIS"/>
    <dbReference type="OrthoDB" id="7657at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="430"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ09740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="640" mass="69299" checksum="27AA33B60DCF85EE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J1</accession>
    <name>F0T8J1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1511</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645093.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09742">
        <property type="protein sequence ID" value="ADZ09742"/>
        <property type="gene ID" value="Metbo_1511"/>
    </dbReference>
    <dbReference type="GeneID" id="10277962"/>
    <dbReference type="KEGG" id="mel:Metbo_1511"/>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313006_0_0_2"/>
    <dbReference type="OMA" id="TIYIANG"/>
    <dbReference type="OrthoDB" id="104512at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032109">
        <property type="entry name" value="Big_3_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16640">
        <property type="entry name" value="Big_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02415">
        <property type="entry name" value="Chlam_PMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="SSF49447"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="POMP_repeat"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="837"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Big_3_5" evidence="6">
        <location>
            <begin position="615"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="5">
        <location>
            <begin position="705"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="861" mass="90434" checksum="89A654CC8447FB17" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M9</accession>
    <name>F0T8M9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, alpha subunit</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.79" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643922.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0319"/>
    <dbReference type="EnsemblBacteria" id="ADZ08571">
        <property type="protein sequence ID" value="ADZ08571"/>
        <property type="gene ID" value="Metbo_0319"/>
    </dbReference>
    <dbReference type="GeneID" id="10276756"/>
    <dbReference type="KEGG" id="mel:Metbo_0319"/>
    <dbReference type="eggNOG" id="arCOG01613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113594_0_0_2"/>
    <dbReference type="OMA" id="IPMGQWT"/>
    <dbReference type="OrthoDB" id="80332at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022502">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03845">
        <property type="entry name" value="sulfopyru_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="1">
        <location>
            <begin position="2"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="17643" checksum="6AECD263F961F61C" modified="2011-05-03" version="1">MDSSQAVYDAIKEAGIDFIVSLPCVNLGRLMGMVEEDPTIIHVPVTREEEGFGICAGAFMGGKKPGILMQNSGLGNSVNALASLFELYKIPVLMIMSHRGTEGEFMGAQIPMGKATPMVLDALKIKYFNPKKPEEALNIIPEAWNMAEADGAPVGILLDIKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T8</accession>
    <name>F0T8T8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyrrolo-quinoline quinone repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1536</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1536"/>
    <dbReference type="EnsemblBacteria" id="ADZ09766">
        <property type="protein sequence ID" value="ADZ09766"/>
        <property type="gene ID" value="Metbo_1536"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1536"/>
    <dbReference type="eggNOG" id="arCOG02492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="OMA" id="YMLLLHT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025666">
        <property type="entry name" value="PQQ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="PQQ_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="PQQ_3" evidence="5">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PQQ_3" evidence="5">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="4">
        <location>
            <begin position="484"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="589" mass="64024" checksum="3450D725FB1C819F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W5</accession>
    <name>F0T8W5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645144.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09793">
        <property type="protein sequence ID" value="ADZ09793"/>
        <property type="gene ID" value="Metbo_1566"/>
    </dbReference>
    <dbReference type="GeneID" id="10278022"/>
    <dbReference type="KEGG" id="mel:Metbo_1566"/>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772964_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="358" mass="40595" checksum="F2CF8155CCA3020C" modified="2011-05-03" version="1" precursor="true">MKRNILFLAVLISFFIFVPTASAVDSTDIVICDVNHKYAGNHANLNIEGYVENSGDWDSPDYWGTPDGYVKLKVVGKNFTKESNQYTSNGDFYFSIPVNSGKYSYFVRYAGNSMFGSSSYNNAYTIPKGNSTLSLNMKLGIKKLTLTTKLTDLNSKTPISGIIQTKLGAKTYFTKTNSQGIATFTIPASSGKQNLLVSFIGSNIYNGKNRTLNFSISPNTIIISKNTTTFVKYVEDNNYLYKKYAKITKNYLFSGLVKTSEKYSYQKINSLRTVNFGSGTSHQIVKTYPDGYLEVDKYSYINQIGIYDYINYDNRYLDYIKICYNNGKTKLITCTHYYSDYWIISSIGVSKIKFYFYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X7</accession>
    <name>F0T8X7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645156.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1578"/>
    <dbReference type="EnsemblBacteria" id="ADZ09805">
        <property type="protein sequence ID" value="ADZ09805"/>
        <property type="gene ID" value="Metbo_1578"/>
    </dbReference>
    <dbReference type="GeneID" id="10278034"/>
    <dbReference type="KEGG" id="mel:Metbo_1578"/>
    <dbReference type="HOGENOM" id="CLU_1665506_0_0_2"/>
    <dbReference type="OrthoDB" id="104788at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="19473" checksum="44AB3E2A52B0B4B5" modified="2011-05-03" version="1">MRYSYGILDQKQILLYHPQLFLRNNDVLIFPTREFHKWHGDIKEYLDGLYQIQLRSGTVKKPLSLDMNDFNLANAVLNNITDEVKDIFDENNILNLSHHYVSTLDEKYKKMVGNNYTTDMKRCDTKILLKPRITPWMKEKHVLEIVDQVSSDVVKIKKNEKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T914</accession>
    <name>F0T914_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Quinoprotein glucose dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.5.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643987.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0384"/>
    <dbReference type="EnsemblBacteria" id="ADZ08636">
        <property type="protein sequence ID" value="ADZ08636"/>
        <property type="gene ID" value="Metbo_0384"/>
    </dbReference>
    <dbReference type="GeneID" id="10276821"/>
    <dbReference type="KEGG" id="mel:Metbo_0384"/>
    <dbReference type="eggNOG" id="arCOG02796">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012253_0_0_2"/>
    <dbReference type="OMA" id="RLWRIPL"/>
    <dbReference type="OrthoDB" id="24949at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008876">
        <property type="term" value="F:quinoprotein glucose dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="GSDH" evidence="2">
        <location>
            <begin position="41"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="345" mass="37754" checksum="77ECB07BED09A9C8" modified="2011-05-03" version="1">MEKKYLVAVIIVLILVVVAILVFPKNYNQTGFQSDVIAQNLQVPWAIDFLPNNTMIFTQRDGEVKIIDPAGSETVGKMAVTAQGESGLLGLAVDPDFSNNNYIYVYYTASDGNRVSRFVFNGTLKNETILLDKIPSNNIHNGGRIKFGPDGLLYITTGDAGNNASAQDLNSLAGKVLRMDKNGSVPVENPYNNYVYTYGHRDPQGLAWSSNGTLYESEHGDNHNDEINILVSGGNYGWPLYQGNNTAPGYILPMIVYTDYTLAPSGIAFYNNKLYVAGLRGSQLRQISLTSNGSSFTGQQALFTQLGRIREVVSHDGYLYIATSNTDGRGVPQLGDDKIIRITVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T922</accession>
    <name>F0T922_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name evidence="2" type="ordered locus">Metbo_0392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643995.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0392"/>
    <dbReference type="EnsemblBacteria" id="ADZ08644">
        <property type="protein sequence ID" value="ADZ08644"/>
        <property type="gene ID" value="Metbo_0392"/>
    </dbReference>
    <dbReference type="GeneID" id="10276829"/>
    <dbReference type="KEGG" id="mel:Metbo_0392"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="OrthoDB" id="90765at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="25026" checksum="AF98657BDA7D4F0D" modified="2011-05-03" version="1" precursor="true">MKKKAITVLSGGLDSTVASAKFREDYQIHALTFNYGQKSAEMEIKSAKAVCKELGAEHTVIELPWLAELGNSALTSEESIPEPEDSELDDIESALKTAKSVWVPGRNVVFTAIANSFAEAENASIIIVGWDLEEAVTFPDNSKEFLEAFNTVLEVGSFDVIKIEAPLIGMNKNEIVEYGDSIGAPMELSYSCYKGLNYHCGVCESCMRRKRAFKNTGVEDKTVYSEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T936</accession>
    <name>F0T936_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="6">PTH</shortName>
            <ecNumber evidence="6">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pth</name>
        <name evidence="7" type="ordered locus">Metbo_0406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644009.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0406"/>
    <dbReference type="EnsemblBacteria" id="ADZ08658">
        <property type="protein sequence ID" value="ADZ08658"/>
        <property type="gene ID" value="Metbo_0406"/>
    </dbReference>
    <dbReference type="GeneID" id="10276843"/>
    <dbReference type="KEGG" id="mel:Metbo_0406"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="OMA" id="GHAAVEC"/>
    <dbReference type="OrthoDB" id="115947at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PTHR12649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="SSF102462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="12657" checksum="87A3D1772052EF67" modified="2011-05-03" version="1">MKQIIVMRADLNMSRGKIAAQACHASLGAYKKADEKSSKKWESEGEKKVVLKVNSLEELFEIYELVKVTNMPIYLVHDAGHTEIPKATVTCLGIGPDEDEKIDKITQDLKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T942</accession>
    <name>F0T942_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase M24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0412</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644015.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0412"/>
    <dbReference type="MEROPS" id="M24.008"/>
    <dbReference type="EnsemblBacteria" id="ADZ08664">
        <property type="protein sequence ID" value="ADZ08664"/>
        <property type="gene ID" value="Metbo_0412"/>
    </dbReference>
    <dbReference type="GeneID" id="10276849"/>
    <dbReference type="KEGG" id="mel:Metbo_0412"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_2_2"/>
    <dbReference type="OMA" id="DIYNAMW"/>
    <dbReference type="OrthoDB" id="14436at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="125"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="37310" checksum="591C06E18E541C6D" modified="2011-05-03" version="1">METKLKQVIESMHEQKIDSMIVLKPENIRYLTGFRPTSSSVLILKDEPLLLASKIDNEDASKNSMVNLEVYKKFDDLKNFLEGCVGVENSMTVETYRKLEDFKVVTTEVVETSRAVKTDWEIKNIEKALEIAEKSLLELDFKGTEVEMAAELEYNMRINGSQRPSFETIMASGARTSLPHGSPTTNKIERPILIDWGAVYNNYSSDTTRTIIETEKQEEIFDIVLEAQQTAIKSIKPGIKCGEIDNIAREVITEYGYGESFIHSTGHGVGLEIHEKPSFSKNDETELQKGMVVTVEPGIYIKGEFGVRIEDMVLVENKAKVLNKIDSKLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T956</accession>
    <name>F0T956_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="1">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="1">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tiaS</name>
        <name evidence="5" type="ordered locus">Metbo_0426</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644029.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0426"/>
    <dbReference type="EnsemblBacteria" id="ADZ08678">
        <property type="protein sequence ID" value="ADZ08678"/>
        <property type="gene ID" value="Metbo_0426"/>
    </dbReference>
    <dbReference type="GeneID" id="10276863"/>
    <dbReference type="KEGG" id="mel:Metbo_0426"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OMA" id="GMCTTYL"/>
    <dbReference type="OrthoDB" id="58019at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF1743" evidence="4">
        <location>
            <begin position="145"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="3">
        <location>
            <begin position="274"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="424" mass="48005" checksum="2CA2AE239F8E3306" modified="2011-05-03" version="1">MNDILLYVGIDDTDSSSGMCTTYVTCVIISKLKDYGFNISGYPRLIRLNPFARFKTRGNGALSFKLKLKTEKEVEEVKKLVLENVQTLSELQDEKTNPGVVFYQNDVTLELEEFADRTIRNIVSITETESILKNIGADYYKFKNGRGIIGALAAIGCNLTDQTFELLAYRTPENYGSKRRIDHESVVEMDTKTYPSTFDNLDHGYIAIEPHTPCPVLYGIRGENPDVLEQARTMVRVMEPVERHCIFQTNQHTDMHLKPIDNISDMEKFKCYIVNGKVMDKPHVIDGGHLIFRLEDESGNIECAAYEPTKQFRNYVRKLEPGDILEVYGGVGEGINNQGTLNIEKFQLKELAKTEKFVNPMCSCGKRMKSAGKNKGFKCPSCSKIERNGEKVAVEVPRNIEKGFYETPPSARRHLSKPLVRDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T963</accession>
    <name>F0T963_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP synthase subunit c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0433</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644036.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0433"/>
    <dbReference type="EnsemblBacteria" id="ADZ08685">
        <property type="protein sequence ID" value="ADZ08685"/>
        <property type="gene ID" value="Metbo_0433"/>
    </dbReference>
    <dbReference type="GeneID" id="10276870"/>
    <dbReference type="KEGG" id="mel:Metbo_0433"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126047_0_0_2"/>
    <dbReference type="OMA" id="NFIQLGA"/>
    <dbReference type="OrthoDB" id="119164at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="7">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="7">
        <location>
            <begin position="95"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="15859" checksum="352445FC281C5FFC" modified="2011-05-03" version="1" precursor="true">MVEIVLGTALAAIGAGLAVGLAGLGSGIGQGIAAAGSVGAVAEDPDMFARGIIFTALPETQAIYGFLIAILLLVFSGIMNPSLTLNVSQGIIAVGAGAAIGFAGLGSGMGQGITASSSVGAVVEEPDMFARGIIFTALSETQAIYGFLIAILLMVFAKILGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T984</accession>
    <name>F0T984_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645186.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1608"/>
    <dbReference type="EnsemblBacteria" id="ADZ09835">
        <property type="protein sequence ID" value="ADZ09835"/>
        <property type="gene ID" value="Metbo_1608"/>
    </dbReference>
    <dbReference type="GeneID" id="10278065"/>
    <dbReference type="KEGG" id="mel:Metbo_1608"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="OMA" id="NENGWIE"/>
    <dbReference type="OrthoDB" id="109784at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="528"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="631" mass="68611" checksum="8A9BE24BD835DA47" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B8</accession>
    <name>F0T9B8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1643</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645220.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1643"/>
    <dbReference type="EnsemblBacteria" id="ADZ09869">
        <property type="protein sequence ID" value="ADZ09869"/>
        <property type="gene ID" value="Metbo_1643"/>
    </dbReference>
    <dbReference type="GeneID" id="10278100"/>
    <dbReference type="KEGG" id="mel:Metbo_1643"/>
    <dbReference type="eggNOG" id="arCOG01964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_6_1_2"/>
    <dbReference type="OMA" id="ANIAAWF"/>
    <dbReference type="OrthoDB" id="117326at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="10">
        <location>
            <begin position="134"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="29286" checksum="ABC5813BB63FD294" modified="2011-05-03" version="1">METKFNIRNLFEAFIIILIIADLVMLMLITFTSVSYSIFVSVIYFDLMVCIILFFDFIHRMRKETDKKQFIKRNWPDIIAMIPFDIFALNPTYVFFARFLRIFRLARLFALFRREWKYISSFFKQTHINFAFGMLLFTLFAGTIIFYIVENGSNPSIHSFWDALWFTVTTMVAGNSNISPATYDGEAISVFMMLIGVSFVGVLTASLASWLMSKSKKTEDEREERIKNIETSLQDIKKEITELSDLLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D3</accession>
    <name>F0T9D3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645235.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09884">
        <property type="protein sequence ID" value="ADZ09884"/>
        <property type="gene ID" value="Metbo_1658"/>
    </dbReference>
    <dbReference type="GeneID" id="10278115"/>
    <dbReference type="KEGG" id="mel:Metbo_1658"/>
    <dbReference type="HOGENOM" id="CLU_2678949_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="74" mass="8801" checksum="7B45DD256F40EB2D" modified="2011-05-03" version="1">MTMKLRKQSKNRAINREIHDDVGKDPEELDQFLENIGKKIDETKELIIHELHKIPETGKPEKKTEPRRSLNKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F9</accession>
    <name>F0T9F9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TspO and MBR like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644058.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0455"/>
    <dbReference type="EnsemblBacteria" id="ADZ08707">
        <property type="protein sequence ID" value="ADZ08707"/>
        <property type="gene ID" value="Metbo_0455"/>
    </dbReference>
    <dbReference type="GeneID" id="10276893"/>
    <dbReference type="KEGG" id="mel:Metbo_0455"/>
    <dbReference type="eggNOG" id="arCOG04434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091805_2_0_2"/>
    <dbReference type="OMA" id="RDNQGWR"/>
    <dbReference type="OrthoDB" id="95433at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PTHR10057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005859">
        <property type="entry name" value="PBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18509" checksum="11703FBDEC5CE70D" modified="2011-05-03" version="1">MEKFNIKELPLLIGSLMIPFLVASLGSYFTFSNITTWYAVLAKPFWAPPNWIFGPVWTVLYFLMGIALFLILRKGLYRQDVKFAVLIFGIQLALNLIWSIVFFGAHSLFGGFIVIMFLWIAIFANIIAFGVLSRNAGLLLIPYIIWVSIASYLNYTVYLLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G1</accession>
    <name>F0T9G1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644060.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0457"/>
    <dbReference type="EnsemblBacteria" id="ADZ08709">
        <property type="protein sequence ID" value="ADZ08709"/>
        <property type="gene ID" value="Metbo_0457"/>
    </dbReference>
    <dbReference type="GeneID" id="59558428"/>
    <dbReference type="KEGG" id="mel:Metbo_0457"/>
    <dbReference type="eggNOG" id="arCOG06477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460522_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="592" mass="65796" checksum="D7BA44F604C517F0" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G7</accession>
    <name>F0T9G7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit epsilon</fullName>
            <shortName evidence="1">ACDS complex subunit epsilon</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex carbon monoxide dehydrogenase subunit epsilon</fullName>
            <shortName evidence="1">ACDS CODH subunit epsilon</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhB</name>
        <name evidence="2" type="ordered locus">Metbo_0463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase. The precise role of the epsilon subunit is unclear; it may have a stabilizing role within the alpha(2)epsilon(2) component and/or be involved in electron transfer to FAD during a potential FAD-mediated CO oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644066.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0463"/>
    <dbReference type="EnsemblBacteria" id="ADZ08715">
        <property type="protein sequence ID" value="ADZ08715"/>
        <property type="gene ID" value="Metbo_0463"/>
    </dbReference>
    <dbReference type="GeneID" id="10276901"/>
    <dbReference type="KEGG" id="mel:Metbo_0463"/>
    <dbReference type="eggNOG" id="arCOG04408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123700_0_0_2"/>
    <dbReference type="OMA" id="ITYYYLA"/>
    <dbReference type="OrthoDB" id="109783at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019385">
        <property type="term" value="P:methanogenesis, from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01134">
        <property type="entry name" value="CdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003704">
        <property type="entry name" value="CO_DH_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02552">
        <property type="entry name" value="CO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006035">
        <property type="entry name" value="CO_dh_b_ACDS_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00315">
        <property type="entry name" value="cdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="170" mass="19329" checksum="457145C6BFB53D54" modified="2011-05-03" version="1">MNERVIPWQPTVIAGPKQALLVTPETAKMMMKKAKRPLLVIGPLAKEEPLLTHSIEIAEKWDLPVVTTADTFKIFSERNVETKSYGTVEILNLLKDPEWKGIRGEGQHDLVIFVGGIYYMASQGLSTLKHFAPHLKTLTLCKYFHSNADASFPNMNDEEWSKYLEKMKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R3</accession>
    <name>F0T9R3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1718</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645293.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1718"/>
    <dbReference type="EnsemblBacteria" id="ADZ09942">
        <property type="protein sequence ID" value="ADZ09942"/>
        <property type="gene ID" value="Metbo_1718"/>
    </dbReference>
    <dbReference type="GeneID" id="10278175"/>
    <dbReference type="KEGG" id="mel:Metbo_1718"/>
    <dbReference type="HOGENOM" id="CLU_736957_0_0_2"/>
    <dbReference type="OrthoDB" id="135358at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="375" mass="42221" checksum="650D26EBE7EBC65B" modified="2011-05-03" version="1">MNMRGLYLVEGYPYPDLVELESRGLTHVFYLDNLLLNNYDAVNKNLKNLFKTIENTNLKVIFVLNAFKSSNSDSLVDPTNTAHRNKLKNALSRLIKDFPDLGGVSFKDFEWQTWDGYTSTEKSDILSDFAGEIVSTLKNLNPSIEFSASMNWRSSTLKSVSSKLDYAVTEIFTSNSEGIPITKAIQTVKEYSEGDVVVELLTHDNAVNLDPRSLSDIYNEISTVIANNGPSYLLYASPWIPFGLGFPEVDYSFKEIYMDLNLVSKNKKIPERSSRIMTISFLDQNNDPPSSDLFKTVAGSYKITDQFTGKIIRDFTSFIPKNHVHELILTGEDNQIVNPDVSQENHFITVSIVYGNGRTENNELSVTVLNLLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R7</accession>
    <name>F0T9R7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645297.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09946">
        <property type="protein sequence ID" value="ADZ09946"/>
        <property type="gene ID" value="Metbo_1722"/>
    </dbReference>
    <dbReference type="GeneID" id="10278179"/>
    <dbReference type="KEGG" id="mel:Metbo_1722"/>
    <dbReference type="HOGENOM" id="CLU_2911530_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="61" mass="6913" checksum="34EC58A52E24B460" modified="2011-05-03" version="1">MGDQIQLSPDSILRQVLNNYWAILSTVIAFYFASRVLDNQTAGENVEQEPVEKSEQELGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U3</accession>
    <name>F0T9U3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine--fructose-6-phosphate transaminase (Isomerizing)</fullName>
            <ecNumber evidence="3">2.6.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645323.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1749"/>
    <dbReference type="EnsemblBacteria" id="ADZ09972">
        <property type="protein sequence ID" value="ADZ09972"/>
        <property type="gene ID" value="Metbo_1749"/>
    </dbReference>
    <dbReference type="GeneID" id="10278206"/>
    <dbReference type="KEGG" id="mel:Metbo_1749"/>
    <dbReference type="eggNOG" id="arCOG00058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_2_1_2"/>
    <dbReference type="OMA" id="HCLILAT"/>
    <dbReference type="OrthoDB" id="42859at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="30"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="341" mass="38741" checksum="2A34B8D94D7D8606" modified="2011-05-03" version="1">MEYQMYQEILEQPGALRKTFKEEKVHMTELAAKMDEFDKIYLIGCGSSFSTCCSAKSAVDYISNKDIEVYTGYEFVYNKNTDYKNSAALLTSQSGETADTLAALRLAKQRGVYTVSITNELNSKMAHEADASIITRGGTEKAILGTKTYTTQLMCLYNLLFQMHHETSGDKLDIRKNVLRDIRKLPNITENLIKRTQSEGLRLGEQFKDEELFYCMGSGPNYGLAYKLAMTMFMEGALKHACPVYSGEFRHGLIERAEKNVPIIFLDADYPGDKITKNAIEFGEKLGTKSIVYNMHDYSNMNSLMSPFTLVIPLEWFIYYLAHFNGEDPGETRHIGKIRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V2</accession>
    <name>F0T9V2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644126.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0523"/>
    <dbReference type="EnsemblBacteria" id="ADZ08775">
        <property type="protein sequence ID" value="ADZ08775"/>
        <property type="gene ID" value="Metbo_0523"/>
    </dbReference>
    <dbReference type="GeneID" id="10276968"/>
    <dbReference type="KEGG" id="mel:Metbo_0523"/>
    <dbReference type="HOGENOM" id="CLU_1954682_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="15042" checksum="5DC66A21FD2EC699" modified="2011-05-03" version="1">MGKPWRLNEEIRLELLVNEKGLSLGEIYRSKLFKGRSYTALSMKLHKMKLKSCRKGVKHYTNEEVWKIWIYYKQGLTAPEIGSKLNRKKKSISNQITRFGMAYSKPYIPCPEYLKPKVDAILQKMNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V6</accession>
    <name>F0T9V6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NMD3 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644130.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0527"/>
    <dbReference type="EnsemblBacteria" id="ADZ08779">
        <property type="protein sequence ID" value="ADZ08779"/>
        <property type="gene ID" value="Metbo_0527"/>
    </dbReference>
    <dbReference type="GeneID" id="10276972"/>
    <dbReference type="KEGG" id="mel:Metbo_0527"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="OMA" id="CHMCGSY"/>
    <dbReference type="OrthoDB" id="105851at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="PTHR12746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMD3" evidence="1">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="39617" checksum="7A9C61A3A5B712C9" modified="2011-05-03" version="1">MFCPRCGSDDEELIEGICKSCFIKEAKIVGMDDDLKITICAHCNSILNGIKWEDSELSDEELVSFAVMENLRAMEHVEDLEISVDILTVRGSNYECLVHAEGKLLGTMVVEEYKVNVKIVKNVCPDCSKFASGYFESVIQIRADNRFPTPEELRVVDEIVRTKLGNLSLKNRMAYVSDVAMLKEGVDYYVGSYKAAKKVAETIKDSLGGVMQESPRLVGRDKSRGKDLYRIWISIRMPDFQRGDFVEYEDHKAEVMSFDGKKIGLKNLESGDSLSVMWRDYGKIKVIARKHDVKTTSVTSKTPTSIQILHPDSYQPVDIDINDEISKLMIGNNVKVLEVEGILYILSKDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X3</accession>
    <name>F0T9X3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L31e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl31e</name>
        <name evidence="6" type="ordered locus">Metbo_0544</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ribosomal protein L31e family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644147.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0544"/>
    <dbReference type="EnsemblBacteria" id="ADZ08796">
        <property type="protein sequence ID" value="ADZ08796"/>
        <property type="gene ID" value="Metbo_0544"/>
    </dbReference>
    <dbReference type="GeneID" id="10276989"/>
    <dbReference type="KEGG" id="mel:Metbo_0544"/>
    <dbReference type="eggNOG" id="arCOG04473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="OMA" id="MEERIYT"/>
    <dbReference type="OrthoDB" id="115384at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_L31e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_L31e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_L31e_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="PTHR10956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="SSF54575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="81" mass="9466" checksum="4606FB2D9FEC63A8" modified="2011-05-03" version="1">MERVYVIPLRDVKRVPRTIRSPKAIRIVREFMQRHMKSDDIVIDSAVNEKIWERGIQKVPPKIKVKATKEEDGSVLVTLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z9</accession>
    <name>F0T9Z9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in the regulation of nitrogen fixation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644173.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0571"/>
    <dbReference type="EnsemblBacteria" id="ADZ08822">
        <property type="protein sequence ID" value="ADZ08822"/>
        <property type="gene ID" value="Metbo_0571"/>
    </dbReference>
    <dbReference type="GeneID" id="10277016"/>
    <dbReference type="KEGG" id="mel:Metbo_0571"/>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="OMA" id="LEMAIHT"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12430" checksum="5C195A96F2675C4D" modified="2011-05-03" version="1">MKKILAIIRPDKLEEVKQALEDIGCNGLTIKEVRGRGIQLGITESYRGHDYKVDLLPKTEIEIVTGEEDLDNIVDTIVKTAQTGDIGDGKIFISPVEEVIRIRTGERGEKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA09</accession>
    <name>F0TA09_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isopropylmalate/isohomocitrate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.85</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0581</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644183.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0581"/>
    <dbReference type="EnsemblBacteria" id="ADZ08832">
        <property type="protein sequence ID" value="ADZ08832"/>
        <property type="gene ID" value="Metbo_0581"/>
    </dbReference>
    <dbReference type="GeneID" id="10277026"/>
    <dbReference type="KEGG" id="mel:Metbo_0581"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="OrthoDB" id="33452at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="4">
        <location>
            <begin position="3"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="35332" checksum="BA8694C2A5ADFB93" modified="2011-05-03" version="1">MYTLTVIPGDGVGPEVTDAVLHIIDALDLNFDYTTADAGEECYKKTGTTIPEETISLAKKSDATLFGAVTTVPGMKSAIITLRKELGLYANLRPVKSYDGIDSLYKDLDILIVRENSEGLYSGIEEYTPEGAIATRVITKVASERICRFAFDQSIKLGRKKVTAVHKANVLKKTDGIFRGEFLKVSEEYPEIETDEKYVDAAAMFMVSKPQIFDVMVTTNLFGDILSDEGAGLVGGLGVAPSANIGDDNALFEPVHGSAPDIAGKGISNPSAMILSAVMMLDYLGEAEQAQELENALLDVLSSSKVTTPDLGGSSKTIEMANEIRSKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA37</accession>
    <name>F0TA37_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645338.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09987">
        <property type="protein sequence ID" value="ADZ09987"/>
        <property type="gene ID" value="Metbo_1764"/>
    </dbReference>
    <dbReference type="GeneID" id="10278221"/>
    <dbReference type="KEGG" id="mel:Metbo_1764"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OMA" id="FHAGIHY"/>
    <dbReference type="OrthoDB" id="99962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="16691" checksum="90E04DAA3B8E45B8" modified="2011-05-03" version="1">MYSKILITTDGSENAEKAANHALWIANESDAEILVLNVFELYSPTLVVPFSTIPGSNQDMYEPLKKEAENISAKFIEKLRSSENFNEETDITMVVRDGKPYQEILKTVEEMDVDLIVMGASGRHGFDRIALGSVTERVVRSAKVPVMVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA74</accession>
    <name>F0TA74_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645375.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1802"/>
    <dbReference type="EnsemblBacteria" id="ADZ10024">
        <property type="protein sequence ID" value="ADZ10024"/>
        <property type="gene ID" value="Metbo_1802"/>
    </dbReference>
    <dbReference type="GeneID" id="10278259"/>
    <dbReference type="KEGG" id="mel:Metbo_1802"/>
    <dbReference type="eggNOG" id="arCOG02527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_810410_0_0_2"/>
    <dbReference type="OMA" id="QYPAISG"/>
    <dbReference type="OrthoDB" id="14198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027618">
        <property type="entry name" value="Beta_prop_Msarc"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04275">
        <property type="entry name" value="beta_prop_Msarc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="342" mass="37161" checksum="42B9A0552B01F0C7" modified="2011-05-03" version="1" precursor="true">MYNKLGMVIITLIFAFVLCGAASAATLKTNHTVPTKTCTVGSSAYDQDYAAIDGSRVVWQQTDSSGHSSIYYKNVVTGYTGKVFNSNYNQFSPAISGTCVVWSQNKTKGIFSIYYKNMATGYCTGVMNSTFSQYAPSISGKTVVWVQNDTSGNFSVYYKNFSTGKYGKVSKNSQVVSRPSISGSRVVWSQVVASSTHAVVYEKNFSTGFYGQLSSSTQDQLNPVISGSRVVWVQISGNVGVIYMKNLSSGTISKVNPSSNLQYNVAVSDQRIIWTQIQGQSSVIYLKNLATGYLAPVVPVTQNPVDNAAISGVRIVWTEYKSDGHTVLYMKNLSTGYKGRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD9</accession>
    <name>F0TAD9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644242.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0640"/>
    <dbReference type="EnsemblBacteria" id="ADZ08891">
        <property type="protein sequence ID" value="ADZ08891"/>
        <property type="gene ID" value="Metbo_0640"/>
    </dbReference>
    <dbReference type="GeneID" id="10277085"/>
    <dbReference type="KEGG" id="mel:Metbo_0640"/>
    <dbReference type="eggNOG" id="arCOG02472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="OMA" id="CKLNEYE"/>
    <dbReference type="OrthoDB" id="44934at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="12"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="303" mass="33289" checksum="9DA1159D0DD373E9" modified="2011-05-03" version="1">MVNIALELLASCTGCEISILDLHEELIDVLEQADLVYAPILMDVKEIPENIDIAIVSGSVRNEENKERLEEIREKAKIVIAYGTCACYGGITCMADLYSPEDVSNRSFKDNPSTESAPEPSEVVPKLLPIVHPAGDLALIDYYLPGCPPKEYLVKDILVPLINGEEPVIPKKSVCADCERTMEHVEFDKIHRRVEGTPDPDLCFLSQGYICMGSATLGRCGALCSSAGVPCHGCGGPSLDILREPNHDVYNCIVNRIAHLSKMPHKDVEKQIYDVGHVIYGFVIGSTIMENKKVSLIPQLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ2</accession>
    <name>F0TAJ2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645415.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1842"/>
    <dbReference type="EnsemblBacteria" id="ADZ10064">
        <property type="protein sequence ID" value="ADZ10064"/>
        <property type="gene ID" value="Metbo_1842"/>
    </dbReference>
    <dbReference type="GeneID" id="10278299"/>
    <dbReference type="KEGG" id="mel:Metbo_1842"/>
    <dbReference type="HOGENOM" id="CLU_1954684_0_0_2"/>
    <dbReference type="OrthoDB" id="122551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="15172" checksum="D9D76B9D3093030C" modified="2011-05-03" version="1">MSLDENINDEITFNKICPKCGVANPDNEANCIICDKDLLETVLYLEDAFFDLEITETEFVEYRKNYYRTRRTGKIKRFKLKKMEEISLGQPIKRINFIYDGKTETYPLKDENYDLLKDFMVKNGYITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ5</accession>
    <name>F0TAJ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645418.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1845"/>
    <dbReference type="EnsemblBacteria" id="ADZ10067">
        <property type="protein sequence ID" value="ADZ10067"/>
        <property type="gene ID" value="Metbo_1845"/>
    </dbReference>
    <dbReference type="GeneID" id="10278302"/>
    <dbReference type="KEGG" id="mel:Metbo_1845"/>
    <dbReference type="eggNOG" id="arCOG01790">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091228_3_0_2"/>
    <dbReference type="OMA" id="FDIVIMH"/>
    <dbReference type="OrthoDB" id="45753at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="36"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25671" checksum="D35DB2AD4E2C9E81" modified="2011-05-03" version="1">METGSGWKQFVGKQTPSTVELNGLFFNKVPTETKVVDFGCAWGRVGFELQKKGYHVCGFDINEHSIEYASEQAKKTNNKYPGKIDFITADALNLPYETNYFGACIMQAFLTTVTNPEARNEVITEAWRVLAPGGILYLADFGQNWNNPIYKARYLRDYSSTGEKGTFFVTEDGKEGGKELFLVHHYTLEELKQLLKNRFAIETTLSTTFKTFHGNNTLGYIIVAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN4</accession>
    <name>F0TAN4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VWA containing CoxE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1885"/>
    <dbReference type="EnsemblBacteria" id="ADZ10106">
        <property type="protein sequence ID" value="ADZ10106"/>
        <property type="gene ID" value="Metbo_1885"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1885"/>
    <dbReference type="eggNOG" id="arCOG06502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716918_0_0_2"/>
    <dbReference type="OMA" id="DCRDWAG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011195">
        <property type="entry name" value="UCP010256"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008912">
        <property type="entry name" value="Uncharacterised_CoxE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05762">
        <property type="entry name" value="VWA_CoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010256">
        <property type="entry name" value="CoxE_vWa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="43833" checksum="4851BC1268E2E39B" modified="2011-05-03" version="1">MKEIVNFSKVLREANFPVSVRTTQAACETYEIFKGDDENLKEALRAVYVKDKVQDKKFDKIFKSVFKGEDGDKSEEVKGADKGSNKTRKFLKAYNYKIRVQDSKPMKINLNESDITDYRPPLDDYLKPPLDDNELMKRDIKHLTTFEPELLDLCQRLGKKIANKRVRRQKLASNVHPDIRKTIRKNLKNGGALIEVVKTKPRIKKSRHFFLNDVSGSCDWVSNWFFCMVYAALNSFKLARTFEFDNKTIETTAALKEPNILDAFIKVRDMRQKNLMIHGTSNMYRAFKEFETVAKLNNKSFVLVLSDCRDWAGPKSEGKPLSSELMEYMVNKSKKVLILNPEPSNKWNIADSCVSDYEAVGAHVHEVRNLEQLADLITLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ5</accession>
    <name>F0TAQ5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1907</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645478.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1907"/>
    <dbReference type="EnsemblBacteria" id="ADZ10127">
        <property type="protein sequence ID" value="ADZ10127"/>
        <property type="gene ID" value="Metbo_1907"/>
    </dbReference>
    <dbReference type="GeneID" id="10278365"/>
    <dbReference type="KEGG" id="mel:Metbo_1907"/>
    <dbReference type="eggNOG" id="arCOG00861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_5_2"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="OrthoDB" id="20371at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="349"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="43880" checksum="F16B76CDCFA6BBCF" modified="2011-05-03" version="1">MEIIVAKFGGTSIGNGDRIRKAAESVVKEYMKGKKVVVVVSAINKTTDDILKTVDDAIGESITPKQLADIVSMGEITSVRIFSSTIESLGVKSEYIDPRAEGWPIITDSDYLNADVNFELTEKKCEELIKILDQGIIPVLCGFVGRDEDGTITTLGRGGSDITAFLLGHCLKAEEVVIVTDVGGVMSTDPNRLQSAKKLDKISVEEMRDLATHGAKVLHPHALKYKDPLINAKIIGFEHGDLSAAGTEIIGPAGNHMLKTTALNVEPISVIAVVGEEILTKTGVLSELTDALAQNSINIYGISTGQNSVTLFIDKSIVDRAHNILHEVVVENQDLSSISVGTDIAMITVTSQDFIDTPGIITRITEPLRKKKINIVEISSSQTSVVVFVEWKDGKRAYELVRSVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ9</accession>
    <name>F0TAQ9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0288 protein Metbo_0684</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0288 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644286.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0684"/>
    <dbReference type="EnsemblBacteria" id="ADZ08935">
        <property type="protein sequence ID" value="ADZ08935"/>
        <property type="gene ID" value="Metbo_0684"/>
    </dbReference>
    <dbReference type="GeneID" id="10277129"/>
    <dbReference type="KEGG" id="mel:Metbo_0684"/>
    <dbReference type="eggNOG" id="arCOG04900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533841_0_0_2"/>
    <dbReference type="OMA" id="FKKWDVV"/>
    <dbReference type="OrthoDB" id="20383at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01089">
        <property type="entry name" value="UPF0288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016466">
        <property type="entry name" value="UPF0288_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005852">
        <property type="entry name" value="UCP005852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03268">
        <property type="entry name" value="methan_mark_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="512" mass="57208" checksum="B48E614C2CE0F28E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS6</accession>
    <name>F0TAS6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CoB/CoM heterodisulfide reductase, subunit B</fullName>
            <ecNumber evidence="6">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0701</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644303.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0701"/>
    <dbReference type="EnsemblBacteria" id="ADZ08952">
        <property type="protein sequence ID" value="ADZ08952"/>
        <property type="gene ID" value="Metbo_0701"/>
    </dbReference>
    <dbReference type="GeneID" id="10277146"/>
    <dbReference type="KEGG" id="mel:Metbo_0701"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OMA" id="PGCVAQG"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="5">
        <location>
            <begin position="147"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="298" mass="32706" checksum="308D092C5E64FD24" modified="2011-05-03" version="1">MAFAYFLGCIMNNRYPGIEKATRVMFDKLGVELNDMEGASCCPAPGVFGSFDKTTWAAIAARNITIAEDMNSDIMTECNGCFGSLYETNHLLKEDEKMKEKINGVLSEAGREFKGEVNVRHFAEILYNEVGVDKLSESVEKPLNLNVAVHYGCHFLKPSAEINIDDPMKPTILDELVEITGAKSIAYKDKMMCCGAGGGLRSRDIDVTLDYTKEKLDNMTAAGVDAIVNVCPFCHLQFDVGQTEVNKKYGTDFAIPVFHLAQLYGLAMGLKSEELTVDAHLTSTDPALKKLDEITGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT1</accession>
    <name>F0TAT1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644308.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0706"/>
    <dbReference type="EnsemblBacteria" id="ADZ08957">
        <property type="protein sequence ID" value="ADZ08957"/>
        <property type="gene ID" value="Metbo_0706"/>
    </dbReference>
    <dbReference type="GeneID" id="10277151"/>
    <dbReference type="KEGG" id="mel:Metbo_0706"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_2_2"/>
    <dbReference type="OMA" id="EDVVFAF"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14488" checksum="2184B72A956E1A03" modified="2011-05-03" version="1">MMLNELKVEDVMSSKVITVSPSEDVVFAFEKLMKHKISSLPVVEDNKLMGIVTATDLGHNLVLDKYEIGTIVEKVMVTDVVCVKPDDDLLTAVKKMNEHGSDEEIINQLVVCSGDKIKGIISDGDIIKSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW0</accession>
    <name>F0TAW0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644337.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0735"/>
    <dbReference type="EnsemblBacteria" id="ADZ08986">
        <property type="protein sequence ID" value="ADZ08986"/>
        <property type="gene ID" value="Metbo_0735"/>
    </dbReference>
    <dbReference type="GeneID" id="10277182"/>
    <dbReference type="KEGG" id="mel:Metbo_0735"/>
    <dbReference type="eggNOG" id="arCOG08027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_603589_0_0_2"/>
    <dbReference type="OMA" id="IYESFEV"/>
    <dbReference type="OrthoDB" id="58216at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="453" mass="50931" checksum="02B20E748319181D" modified="2011-05-03" version="1" precursor="true">MINLLVTIFGGLLLIAGFFGMSFGFATPPDFGLFIVGLISSVFGIILMIFFSGRINLSSKSKKPKKLPKQTVKPVMTKEKKGPIERIEKSIKPPAKSKTTPKIKPKPSPEVSKASKASKASPERPEKQLKKIEPIVSRPVPKKVNTSEEKEILEKTTKPPELSGQTKKPVTVARGLEIQNKEPSKVKPTPKPVTSQPRIVPTPVNKKQPEKTEDNKKSSPVKPVLLKETTNNSETVEDKPKPVPKHVDVSKLNLKTKPASDREDQEFVKNRLNRLKENYIKNAKDIESIIDERLDSFKGTLDTLKSESQEPSIIWSFDAGDVQDALKDTISKANEKVLIMYPWIRNIDVAILKKFMETDSRMIIQEASLDDDTSVELIKLLEENEVKIRTMPHIHTVAVVSDNTNGLIISTDPIYESFEVGVMYKDQKSIEEIERLFEDAWSISKDLDMELKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX1</accession>
    <name>F0TAX1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR locus-related DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644348.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08997">
        <property type="protein sequence ID" value="ADZ08997"/>
        <property type="gene ID" value="Metbo_0746"/>
    </dbReference>
    <dbReference type="GeneID" id="10277193"/>
    <dbReference type="KEGG" id="mel:Metbo_0746"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363668_0_0_2"/>
    <dbReference type="OMA" id="TYNHIRE"/>
    <dbReference type="OrthoDB" id="68515at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="DUF6293"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6293" evidence="1">
        <location>
            <begin position="10"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="22132" checksum="43032CA05A1FE73A" modified="2011-05-03" version="1">MDYTLISTIYSIEPIMVCITQFSPKKVVLLREEDAPQAKLKVEQIISETVGNFIDIETKITSLYDVVRIAKDTVDVIENEKANNRKLFVNISGGRKPQALGALFGCYARHGDIERIVYVTEEDSQTIDLPILNFGLSPTKRMVLEELTKDGKSVKNLAIRIGISRGMTYNHIRELREMGFISPDQLEITTAGKLAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY6</accession>
    <name>F0TAY6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Redox-active disulfide protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645483.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1912"/>
    <dbReference type="EnsemblBacteria" id="ADZ10132">
        <property type="protein sequence ID" value="ADZ10132"/>
        <property type="gene ID" value="Metbo_1912"/>
    </dbReference>
    <dbReference type="GeneID" id="10278370"/>
    <dbReference type="KEGG" id="mel:Metbo_1912"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_20_2_2"/>
    <dbReference type="OMA" id="YGIMAVP"/>
    <dbReference type="OrthoDB" id="110723at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004502">
        <property type="entry name" value="Thio_glut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00411">
        <property type="entry name" value="redox_disulf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="86" mass="9442" checksum="DD2F359DC242D9C0" modified="2011-05-03" version="1">MVVKVEVFTSPSCPYCPMAVQLVEEVKKEMTEDLEVDKIDIMVDQEKAREYGLMAVPAIALNGVVRFVGAPGKEELIEAIKEASKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY8</accession>
    <name>F0TAY8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moaC</name>
        <name evidence="5" type="ordered locus">Metbo_1914</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645485.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1914"/>
    <dbReference type="EnsemblBacteria" id="ADZ10134">
        <property type="protein sequence ID" value="ADZ10134"/>
        <property type="gene ID" value="Metbo_1914"/>
    </dbReference>
    <dbReference type="GeneID" id="10278372"/>
    <dbReference type="KEGG" id="mel:Metbo_1914"/>
    <dbReference type="eggNOG" id="arCOG01530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OMA" id="IWDMVKS"/>
    <dbReference type="OrthoDB" id="104538at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="Mo_CF_biosynth-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaC" evidence="4">
        <location>
            <begin position="16"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="156" mass="17135" checksum="B6C835D81229F572" modified="2011-05-03" version="1">MDNQEFTHVTQKGVRMVDVGDKAITKRSAKASGRIYLQEFTIAKIIKEEIKKGNVLTTAQIAAISAVKSTHHLIPLCHSLKITGVDVDFQVDTEFIDVEVTVKCEGKTGVEMEALTGASVGLLTIWDMVKSVEKDENGQYPSTKISDIVVTEKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB15</accession>
    <name>F0TB15_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="9">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_1941</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645512.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1941"/>
    <dbReference type="EnsemblBacteria" id="ADZ10161">
        <property type="protein sequence ID" value="ADZ10161"/>
        <property type="gene ID" value="Metbo_1941"/>
    </dbReference>
    <dbReference type="GeneID" id="10278401"/>
    <dbReference type="KEGG" id="mel:Metbo_1941"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="18705at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:N6-threonylcarbomyladenosine methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061712">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="134"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="10">
        <location>
            <begin position="366"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="425" mass="47698" checksum="D9A4710213A1B750" modified="2011-05-03" version="1">MKVYIDTVGCTFNQADSQIMAGILKENRVELVDTPEDADTIIMNTCYVKHPTEQKVLTKIKKMQEQYPESKLLISGCMVEIDPEKLADAAPEASWIGPHKIKSTYEIVNSVHEGNIVRETGFSSEPKVGLPKVRTNPIIHIIQICEGCDGFCTYCCTRFARGRIQSYSSEMIKKEAEQAVTEGCKEIQLTAQDTAAYGRDTGESLADLISMISDIDGKFKLRVGMMHPKSIMNQVDPIIKAFKKGKCYKFLHLPIQSGSDTVLHDMNRCHTVDEYKTIVSRFREEIPDISISTDIIVGYPTETDDDFKATLNLIKELEPDFLHISKYMHRPGTTSSQLEEIEHETMKERSKALNDLKMEIAMKKNSMMIGSKQTILVTNKGRKGGYVGRSDGYKTVIIEAAEIGSFVDVVIKDAKPTYLLAELDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB58</accession>
    <name>F0TB58_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644360.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0758"/>
    <dbReference type="EnsemblBacteria" id="ADZ09009">
        <property type="protein sequence ID" value="ADZ09009"/>
        <property type="gene ID" value="Metbo_0758"/>
    </dbReference>
    <dbReference type="GeneID" id="10277206"/>
    <dbReference type="KEGG" id="mel:Metbo_0758"/>
    <dbReference type="eggNOG" id="arCOG06506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172996_0_0_2"/>
    <dbReference type="OMA" id="VRIEIFL"/>
    <dbReference type="OrthoDB" id="122463at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="13189" checksum="6487740A16EFD9DD" modified="2011-05-03" version="1">MATKKNDTITIDKEFKARMKAFKEALKSEEKKTELHESIYGSEVLIRFEVFLPSRDPLKFADGLFLYMNDEGEIVDAEYYIKIENNITVSKLKPKDLDVVKELFKDSFSLEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC8</accession>
    <name>F0TBC8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644430.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0828"/>
    <dbReference type="EnsemblBacteria" id="ADZ09079">
        <property type="protein sequence ID" value="ADZ09079"/>
        <property type="gene ID" value="Metbo_0828"/>
    </dbReference>
    <dbReference type="GeneID" id="10277277"/>
    <dbReference type="KEGG" id="mel:Metbo_0828"/>
    <dbReference type="eggNOG" id="arCOG03183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_16_0_2"/>
    <dbReference type="OMA" id="FLIEQRW"/>
    <dbReference type="OrthoDB" id="96538at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="19719" checksum="0FF33859ED6D0E73" modified="2011-05-03" version="1">MKNSDELMAEHAAGPRLDMEKYILVVLFLIQQRWTYTINHEFKRDNITTKQWLMLIVLSTAFETPPSMQEVADAMSITHQNVKQLAVRLESQGFIKIERDPKNKRILRLVPTEKSNEYWAKRESDHARSIQEYFKDLNNEEVLSLFQIMGKLEKLSGQMYLETKNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD1</accession>
    <name>F0TBD1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Adenylosuccinate synthetase</fullName>
            <shortName evidence="7">AMPSase</shortName>
            <shortName evidence="7">AdSS</shortName>
            <ecNumber evidence="7 8">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purA</name>
        <name evidence="9" type="ordered locus">Metbo_0831</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="7 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644433.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0831"/>
    <dbReference type="EnsemblBacteria" id="ADZ09082">
        <property type="protein sequence ID" value="ADZ09082"/>
        <property type="gene ID" value="Metbo_0831"/>
    </dbReference>
    <dbReference type="GeneID" id="10277280"/>
    <dbReference type="KEGG" id="mel:Metbo_0831"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="35648at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="37039" checksum="01940001E0D22865" modified="2011-05-03" version="1">MTCNVLVGGGWGDEGKGKCITYLCYNDKPDIIARAGVGPNAGHSVEFNGEKYGLRMIPSGFVHTGARLLIGAGVLVDPEVFHHELDYLNKYQVKNRTFADFRSAIIEPKHKEQDRASDYLSKKIGSTGSGCGPANSDRVMRTAKLAGDIPEMQGYTADVPTEINEALDEGKEVFVEGSQGFGLSLYYGTYPYVTSKDTTASSAAADVGIGPTRVDDVIVVFKSYITRVGEGPFKTEISQQKAEDLDIEEYGTVTGRRRRVGTFDMELAKESCMINGATQIALTCVDRIFPNCERVQDYSQLSQEVKNFVAEIEGETGVPVTIISTGPDLVDTIDLRDELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG4</accession>
    <name>F0TBG4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645584.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2014"/>
    <dbReference type="EnsemblBacteria" id="ADZ10233">
        <property type="protein sequence ID" value="ADZ10233"/>
        <property type="gene ID" value="Metbo_2014"/>
    </dbReference>
    <dbReference type="GeneID" id="10278474"/>
    <dbReference type="KEGG" id="mel:Metbo_2014"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OMA" id="MINLVDV"/>
    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="65"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="173" mass="19793" checksum="13652F4ACA39A853" modified="2011-05-03" version="1">MINLVDVLEKLRELEKLGGLKFMVLHVLDKGPKNGTEIMDSIERHRIQVNKVLNRHSHDENAEEICSSLKPSSGAIYPLLKKLVSKGLIVKREDNKYELTEKGSYTLEKISGHLHHSLNEPMNRGTIMVDTALNEIESYIRFLSDIKPEKLQSKKESISKMSKMLIELEKSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU4</accession>
    <name>F0TBU4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645637.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10286">
        <property type="protein sequence ID" value="ADZ10286"/>
        <property type="gene ID" value="Metbo_2067"/>
    </dbReference>
    <dbReference type="GeneID" id="10278529"/>
    <dbReference type="KEGG" id="mel:Metbo_2067"/>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392911_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="93" mass="10286" checksum="C01FAD94E78FE3ED" modified="2011-05-03" version="1" precursor="true">MGQYEFCMNFRRDHSNLMVFTVVISLLGVIFASGMGNVSAASGDTSYDATPKNGGAIYNDGSLNVNNCTFTNNKATDVGAIILWWSYQRPWKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX8</accession>
    <name>F0TBX8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645671.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2101"/>
    <dbReference type="EnsemblBacteria" id="ADZ10320">
        <property type="protein sequence ID" value="ADZ10320"/>
        <property type="gene ID" value="Metbo_2101"/>
    </dbReference>
    <dbReference type="GeneID" id="10278564"/>
    <dbReference type="KEGG" id="mel:Metbo_2101"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="OMA" id="GYDVHMN"/>
    <dbReference type="OrthoDB" id="119206at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00257">
        <property type="entry name" value="Lsm_RuxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="LSM_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="PTHR10553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="8922" checksum="910DC25D00AED99E" modified="2011-05-03" version="1">MTVQKNLNTSRPLDVLGKSLNSQVLIELKGGREFRGLLKSFDMHMNLVLNEAEELDGLETAKRLGIVLIRGDNIVYISPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY4</accession>
    <name>F0TBY4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645677.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2107"/>
    <dbReference type="EnsemblBacteria" id="ADZ10326">
        <property type="protein sequence ID" value="ADZ10326"/>
        <property type="gene ID" value="Metbo_2107"/>
    </dbReference>
    <dbReference type="GeneID" id="10278570"/>
    <dbReference type="KEGG" id="mel:Metbo_2107"/>
    <dbReference type="eggNOG" id="arCOG01865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_3_2_2"/>
    <dbReference type="OMA" id="YDSVVPH"/>
    <dbReference type="OrthoDB" id="107663at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="21287" checksum="BF716F56186364BE" modified="2011-05-03" version="1">MSENRVGEKIRQLRESKNISIEELAENSDLSVEMVEKLESNTVMPSLSPLLKIAKALDVRLGTFLDDAPQSGPVVVKSGKSENVMRFSGKNTDLKESTLEFYSLASGKTDRHMEPFIIDVHPPENEDYQLSSHEGEEFIYVMNGEIEVLYGKEKYLVSKGDSIYYDSVVPHDLHAYGGEAKILAIVYTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC05</accession>
    <name>F0TC05_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="2">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="2">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="2">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">uppS</name>
        <name evidence="3" type="ordered locus">Metbo_2128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645698.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2128"/>
    <dbReference type="EnsemblBacteria" id="ADZ10347">
        <property type="protein sequence ID" value="ADZ10347"/>
        <property type="gene ID" value="Metbo_2128"/>
    </dbReference>
    <dbReference type="GeneID" id="10278591"/>
    <dbReference type="KEGG" id="mel:Metbo_2128"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_2_0_2"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="OrthoDB" id="50844at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="255" mass="29720" checksum="6433F8A5F60A025D" modified="2011-05-03" version="1">MSILKPVYKVYEWYISRNLSPENMPKHVAIIMDGNRRFSRIQGNIDAIEGHKRGIGTLEKFLDWCVDLEIEIVTVYAFSIENFNRTEQEVRGLMNLFKENFEGIAKNEKIHKNKVRIKAVGKMELLPEDVRKAIEIAEDSTADYHERLVNIAIGYDGRLEIVDAIKKIVKDVEDGKVQIDDIDEKLVNQNLYTAGLDDPNLIIRTSGEERLSGFLLWQSSYSELYFCDSLWPELRKVDFLRALRSYQQRERRFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC62</accession>
    <name>F0TC62_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="3">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tgtA</name>
        <name evidence="5" type="ordered locus">Metbo_0966</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>7-cyano-7-deazaguanine + guanosine(15) in tRNA = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0966"/>
    <dbReference type="EnsemblBacteria" id="ADZ09213">
        <property type="protein sequence ID" value="ADZ09213"/>
        <property type="gene ID" value="Metbo_0966"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0966"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="OMA" id="FPCSCPV"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="604"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="678" mass="76200" checksum="05148D64B1CF018C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC63</accession>
    <name>F0TC63_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0967"/>
    <dbReference type="EnsemblBacteria" id="ADZ09214">
        <property type="protein sequence ID" value="ADZ09214"/>
        <property type="gene ID" value="Metbo_0967"/>
    </dbReference>
    <dbReference type="GeneID" id="10277416"/>
    <dbReference type="KEGG" id="mel:Metbo_0967"/>
    <dbReference type="eggNOG" id="arCOG00120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052777_0_0_2"/>
    <dbReference type="OMA" id="ICYGLGY"/>
    <dbReference type="OrthoDB" id="42522at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008471">
        <property type="entry name" value="MnmC-like_methylTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05430">
        <property type="entry name" value="Methyltransf_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_30" evidence="1">
        <location>
            <begin position="174"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="427" mass="47676" checksum="E3BA5242BDD3AE49" modified="2011-05-03" version="1">MTTNEPYEPKVIESNVLEIVENCFISENNGNLNARAEAMKDLESFLVETNEGSYTIKCQDSEETMHTYHGGINESIEKYVKPSHLNEKREMKILDVCSGVGYTAATCIEQLNQPREDGGLPLLNIEMVEISALTLATGLILPSPIKSHMIIKKAIEDQLYSEGFLTNRLVNTKIPENINISVNITDARNLVKNDPNGNGTSKNFVKDNAHQFDAIFLLAFSPFESPELYSLEFLSGLKPLLKPDGMISSFSKSHTMRYSLVKAGYHIGEGPEFGRSGGTIASTALELIEKPISIDDERVVALSDAGVPLRDLTLGDSSLEILERRDEERENVRGKFKFPSTVRCPVFLGKDLKESRLKRRVLNGLKTIGIDNLHSQKSMYLICPQYQDCVCGNSCKPIDNSRDRIIEMEKRLNILAVNQLSSKKNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC74</accession>
    <name>F0TC74_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lactate utilization protein B/C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644576.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0978"/>
    <dbReference type="EnsemblBacteria" id="ADZ09225">
        <property type="protein sequence ID" value="ADZ09225"/>
        <property type="gene ID" value="Metbo_0978"/>
    </dbReference>
    <dbReference type="GeneID" id="10277427"/>
    <dbReference type="KEGG" id="mel:Metbo_0978"/>
    <dbReference type="eggNOG" id="arCOG00335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_3_0_2"/>
    <dbReference type="OMA" id="HCPVYDK"/>
    <dbReference type="OrthoDB" id="43344at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019516">
        <property type="term" value="P:lactate oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="4Fe-4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="PTHR47153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="400" mass="43800" checksum="9F52C3B6E2AE7F87" modified="2011-05-03" version="1">MKQSEIKAMNRSFGILDGRKSNILHSKRTEELKERVNQIRKFSIDNMEELVSRGIEKLEENGVEVVTADKPEDALEAIYSIVHDQSIVAKSKSNTAGEIGLTEFLEFKGVSVIETDLGDRIVQLDPNSRPSHPIGPASHLRMDDIAVILSNHYGVDVEPEPRTILNTVKDDVVKNISKCSVGITGANSVAADDGSIVTVHNEGNISLVSMLDTHIILVGIDKFVETIEDAVSVVKLETIYASGKTVPAYMNIISSPSKTADIEQILLKNMYGSRRVVVVLLDNGRRKAVEEGGECLLCIGCGSCIVTCPIYNVVGNDFGYRRHLGGRGVVLSSFIDDEKTCKDSGLYLCTMCGQCTYECPVGVKTSELIKKLRNDAVIMGLACDEHRIIRENIKKKGSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC76</accession>
    <name>F0TC76_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644578.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0980"/>
    <dbReference type="EnsemblBacteria" id="ADZ09227">
        <property type="protein sequence ID" value="ADZ09227"/>
        <property type="gene ID" value="Metbo_0980"/>
    </dbReference>
    <dbReference type="GeneID" id="10277429"/>
    <dbReference type="KEGG" id="mel:Metbo_0980"/>
    <dbReference type="eggNOG" id="arCOG00528">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137964_2_0_2"/>
    <dbReference type="OMA" id="EICAFNG"/>
    <dbReference type="OrthoDB" id="89010at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="129" mass="15023" checksum="FDE08F588A8B70EB" modified="2011-05-03" version="1">MNEIVKFIKDAGVFYIATIDGDKPRVRPFGFTMEYQKRIYFCTSNKKNVYNQLKSNPYFEACTLSKDMRWIRLQGKAVFDSSTDAKAKAFEVMPGLAKIYTTEDFEVFYVEDAEATMYSMKDEPRTINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC81</accession>
    <name>F0TC81_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thymidylate synthase, methanogen type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644583.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0985"/>
    <dbReference type="EnsemblBacteria" id="ADZ09232">
        <property type="protein sequence ID" value="ADZ09232"/>
        <property type="gene ID" value="Metbo_0985"/>
    </dbReference>
    <dbReference type="GeneID" id="10277434"/>
    <dbReference type="KEGG" id="mel:Metbo_0985"/>
    <dbReference type="eggNOG" id="arCOG03214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084975_0_0_2"/>
    <dbReference type="OrthoDB" id="57461at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="2">
        <location>
            <begin position="74"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="215" mass="25115" checksum="2FA2688B533920F3" modified="2011-05-03" version="1">MFTTESQLATDAWKTVLKQIMEFGDELEDENEEFTKELLNVVVKVNDPINSQPPEGYFTIKKFKRCENHFLDPESRLNGTDYGNRIRSHFGFKIGRDVYSIRTDQIESVLVRLKRNSTTRRAVITIFDPSIDHYKDNVPSLVMIDIIIRKNRIYLTAVCRSVDYYGSWIQNFFALRGLLKYLSNHLKVKMGPITIHSVSAHVYKRNYKDVLNIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC82</accession>
    <name>F0TC82_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644584.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0986"/>
    <dbReference type="EnsemblBacteria" id="ADZ09233">
        <property type="protein sequence ID" value="ADZ09233"/>
        <property type="gene ID" value="Metbo_0986"/>
    </dbReference>
    <dbReference type="GeneID" id="10277435"/>
    <dbReference type="KEGG" id="mel:Metbo_0986"/>
    <dbReference type="eggNOG" id="arCOG03944">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196470_0_0_2"/>
    <dbReference type="OMA" id="KEHYIDM"/>
    <dbReference type="OrthoDB" id="127482at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="75" mass="8737" checksum="ED326262E22B4461" modified="2011-05-03" version="1">MERLTLKQYRQMVEEVIEFKELNGEMPAFTIIEGCKISKSVYVNMIETANKFILEMGRNPEIVEISDSSEINFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB2</accession>
    <name>F0TCB2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645730.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2164"/>
    <dbReference type="EnsemblBacteria" id="ADZ10379">
        <property type="protein sequence ID" value="ADZ10379"/>
        <property type="gene ID" value="Metbo_2164"/>
    </dbReference>
    <dbReference type="GeneID" id="10278627"/>
    <dbReference type="KEGG" id="mel:Metbo_2164"/>
    <dbReference type="eggNOG" id="arCOG01245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041884_1_0_2"/>
    <dbReference type="OMA" id="NIMEMID"/>
    <dbReference type="OrthoDB" id="68190at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="114"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="342"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="416" mass="48775" checksum="4D93E718F9DF0F50" modified="2011-05-03" version="1">MNSELIEKWFIKKDLSESTQSLYRLGLKKYCELIGKSPDELLEEAEREEDEGLRLRKRKIDYYLPKYKKHLEEAGNSPQTIKVYLSAVKSFYKTNQIEIPEVSNKVTDICLEKNEGRLLTRDDIQKMIETSPIREKTIIYLMALTGMSQAEVRNLTIQKFLNTVNESVDKKIETIDDLFENEKLLNDVVLIIEITRQKVHYRYQTFIPPEVTRNIVLYLKDRVYGRNDNVRIIDCNKTLFVSNKGEKLTKQGIASNFNRIGKKAGFTANEKGAYGFWRSHGLRKYFISTIINNTGDHILADYLVGHKISPIKRAYWKADPEKLKEKYLEVLPYLSIDEIKVKDFKTKEYKELKEKNKALQAQVDKINKNMEENMKTINLWKEVLPKKFQNLNKDDGKNGNDTVLAKYKIDYQDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC5</accession>
    <name>F0TCC5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645743.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2177"/>
    <dbReference type="EnsemblBacteria" id="ADZ10392">
        <property type="protein sequence ID" value="ADZ10392"/>
        <property type="gene ID" value="Metbo_2177"/>
    </dbReference>
    <dbReference type="GeneID" id="10278640"/>
    <dbReference type="KEGG" id="mel:Metbo_2177"/>
    <dbReference type="eggNOG" id="arCOG00616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_2_0_2"/>
    <dbReference type="OMA" id="LWVDAGF"/>
    <dbReference type="OrthoDB" id="84942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004650">
        <property type="entry name" value="HisA/F-archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00734">
        <property type="entry name" value="hisAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="23962" checksum="87F2D4F92644FCAC" modified="2011-05-03" version="1">MITPVLDLKNGLAVAGKSGKRETYKPLKTVFTNSSDPLKIAEALKTAGYSELYIADLDSIEGTGSNHEIVKKINKTIPVMLDAGITKHQDIEAVVDSAEKIIVATETLESMEEIGQIFSLNSKNSLVLSVDLVGGEVLSKNMKLNFEDVMGIIEKHNPAEVIILDVSRVGTNSGFDEAVVDRFKHTDTQLIVGGGVTKTTIKELGKLGVNKFLVGTAIHNGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD9</accession>
    <name>F0TCD9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L15e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15e</name>
        <name evidence="7" type="ordered locus">Metbo_2192</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645757.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2192"/>
    <dbReference type="EnsemblBacteria" id="ADZ10406">
        <property type="protein sequence ID" value="ADZ10406"/>
        <property type="gene ID" value="Metbo_2192"/>
    </dbReference>
    <dbReference type="GeneID" id="10278655"/>
    <dbReference type="KEGG" id="mel:Metbo_2192"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_0_0_2"/>
    <dbReference type="OMA" id="KYMQELY"/>
    <dbReference type="OrthoDB" id="85023at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_L15e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_L15e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_L15e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="PTHR11847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="181" mass="21295" checksum="BC95F6953F7B0842" modified="2011-05-03" version="1">MYKYIRDAWKNPSESYVKELMQSRAPVWRRESVINRIDRPTRIDRARSLGYKAKKGYVVVRTRVRRGGMRKSRFTAGRKPKRMGVKKITPKKSIQRIAEERVARKYPNLEVLNSYWLWEDGKFKFYEVILVDPNHPSIKSDSKINWICEPQHTNRVFRGLTSAGKKSRGLRGKGKGAEKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ7</accession>
    <name>F0TCJ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1027</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644625.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1027"/>
    <dbReference type="EnsemblBacteria" id="ADZ09274">
        <property type="protein sequence ID" value="ADZ09274"/>
        <property type="gene ID" value="Metbo_1027"/>
    </dbReference>
    <dbReference type="GeneID" id="10277476"/>
    <dbReference type="KEGG" id="mel:Metbo_1027"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="OMA" id="SGKGFMP"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="33"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="15966" checksum="EC3FF5886CAF48A3" modified="2011-05-03" version="1">MKKGMDSKAVIDKMLEDTSKTIESIKNDIEKTIVDYTFVPGKDIIETDDSIIVRVDLAGIKKENIELNLTETNLKVKADFEDELVGEVRGNNRRPTLIRRTVRFPRKVCPDEAEAKFENGLLTVEVPKLEKKQSFTIDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM3</accession>
    <name>F0TCM3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Carbamate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1053</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the carbamate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644651.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1053"/>
    <dbReference type="EnsemblBacteria" id="ADZ09300">
        <property type="protein sequence ID" value="ADZ09300"/>
        <property type="gene ID" value="Metbo_1053"/>
    </dbReference>
    <dbReference type="GeneID" id="10277502"/>
    <dbReference type="KEGG" id="mel:Metbo_1053"/>
    <dbReference type="eggNOG" id="arCOG00863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076278_0_0_2"/>
    <dbReference type="OMA" id="QPMDVAG"/>
    <dbReference type="OrthoDB" id="77437at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008804">
        <property type="term" value="F:carbamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04235">
        <property type="entry name" value="AAK_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003964">
        <property type="entry name" value="Carb_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30409">
        <property type="entry name" value="PTHR30409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000723">
        <property type="entry name" value="Carbamate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00746">
        <property type="entry name" value="arcC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="325" mass="35477" checksum="759CB2792693E773" modified="2011-05-03" version="1">MYICRITEVISLTTKILVSLGGNAILKHTDKGNAEEQFSNTAKTSHVLVNMLKEDYHIILTHGNGPQVGDILLAYDISKDIVPPMPLDVCVAQTQGMIGFMLQLSLENSLIKEGMKKSIVTILTQTIIDKDDPQLNKPSKPIGPFYSVDESEELRKNHDWVIVDDNGRGYRRVVPSPLPVGFVEESSIRTLYNEDKFVIACGGGGIPVMKNEAGNFEGVEAVVDKDHTSSLLASKIGAEVLLILTDVEKVAVNFGKPDQKYLDHLTVQEAKDYLEDGQFPPGTMGPKIKSTIDFIEGGGKKAIITSIEKSIKALNGQAGTIITRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN7</accession>
    <name>F0TCN7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase</fullName>
            <ecNumber evidence="1">6.3.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase</fullName>
            <shortName evidence="1">CPSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small carbamoyl-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645778.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2213"/>
    <dbReference type="EnsemblBacteria" id="ADZ10427">
        <property type="protein sequence ID" value="ADZ10427"/>
        <property type="gene ID" value="Metbo_2213"/>
    </dbReference>
    <dbReference type="GeneID" id="10278679"/>
    <dbReference type="KEGG" id="mel:Metbo_2213"/>
    <dbReference type="eggNOG" id="arCOG01596">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734905_0_0_2"/>
    <dbReference type="OMA" id="YQRITIR"/>
    <dbReference type="OrthoDB" id="36757at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02221">
        <property type="entry name" value="CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043673">
        <property type="entry name" value="CPSase_Archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="3">
        <location>
            <begin position="111"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="379" mass="42015" checksum="90A4C1C541659340" modified="2011-05-03" version="1">MKILFIGARLFDDVALYTKQNTIKTIITESNSESPNLKLSDQFFEVSRGMEEPMKIAIQEEVDGVVPLIGVDGPLVDVAKMKEILEKDHGIPVASSGVKAASICADKIKTKQFFVDNSIETPEFFKLSKNNNANLLTKLLSKSPVVLKQGSGQGGSGIKIVSKFEEVGNYFDGFDETLAEKFLEGYEVSIEVLRWNGKTIPLVPVHKGKTTINGLHPLKKVKKAPLDIDGVDNDLNNEEIRKIAIKIADTLNLEGTADIDLIFDMKTGKNYVIEINARPSGTRYITGAATNVYIMQELVKMVSGVWDPSELRDRIKNYSSIEVPVGSYSSSKNNYKFREFSGENSWIIHGPENHERITIRGDSMANAVKTAEDLKILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ5</accession>
    <name>F0TCQ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645796.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2231"/>
    <dbReference type="EnsemblBacteria" id="ADZ10445">
        <property type="protein sequence ID" value="ADZ10445"/>
        <property type="gene ID" value="Metbo_2231"/>
    </dbReference>
    <dbReference type="GeneID" id="10278697"/>
    <dbReference type="KEGG" id="mel:Metbo_2231"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="OMA" id="PMDAGWE"/>
    <dbReference type="OrthoDB" id="99962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="15833" checksum="626B4C3A7D1C6E76" modified="2011-05-03" version="1">MYKKILLPTDGSEFANKAAEHAIWIANASGAEIIALNVIETSSLVGLPAEDLIVRIKDMLKEEGRQALEHIFEMASKESVEEGELKVTLKTKEGSPADVILKTIEKEDVDLVVIGTSGKHGLDRFLLGSVTEKVVRSAKCPVLAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS6</accession>
    <name>F0TCS6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl10</name>
        <name evidence="2" type="synonym">rplP0</name>
        <name evidence="5" type="ordered locus">Metbo_2252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645817.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2252"/>
    <dbReference type="EnsemblBacteria" id="ADZ10466">
        <property type="protein sequence ID" value="ADZ10466"/>
        <property type="gene ID" value="Metbo_2252"/>
    </dbReference>
    <dbReference type="GeneID" id="10278718"/>
    <dbReference type="KEGG" id="mel:Metbo_2252"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="OMA" id="MAHVAEW"/>
    <dbReference type="OrthoDB" id="73593at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_L10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="50S_L10_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="RL10_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="RL10P_insert"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RL10P_insert" evidence="4">
        <location>
            <begin position="107"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="286"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="36235" checksum="255DBBA6B4A84342" modified="2011-05-03" version="1">MPHVAEWKKDEVANLKDLINSYEVVGMANLADIPAPQLQQMRRTLKDSATLKMSRKTLMSLALNDSEKAQIENLEDYMEGQPALIFTNMNPFKLYKILESSKTPAPAKAGSIAPEDIIVPKGDTAFKPGPVLGELQKAGIPAKIEKGKIVITNDKVIVEAGEVIPRDVASILTRLEIFPLEVGIDLRAAYEAETIYTSDLLTVDEEKTLADVQKAFTQALNLSVEAVIFNKESTPLIIQKAVSQSLNLALNAEILNEKTRDVLLAKAYAQMLALASEVSAKDENAVDDELKEKLSSTAAKVETPEDNNDEPEDEEEAEEEEGDAAAGLGALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S5</accession>
    <name>F0T5S5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Drug resistance transporter, EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1073"/>
    <dbReference type="EnsemblBacteria" id="ADZ09318">
        <property type="protein sequence ID" value="ADZ09318"/>
        <property type="gene ID" value="Metbo_1073"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1073"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="FKIHTFS"/>
    <dbReference type="OrthoDB" id="71650at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="10"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="474" mass="51770" checksum="160ACD857BE4B3CD" modified="2011-05-03" version="1">MMIKEKKWQILVALALGTIMVPINTSIVNVALPFIAVGFNSNVLNVEWVITSYLISLLGLVLFFGSLGDKIGHEKVYLSGLLFFIFTSLLCSLSPSLLYLNIFRALQGIGGAMMISVSLGMVKNAFPSYEWGKSLGIYAMVIATGLAIGPAIGGILIGLFGWQSIFLANLPIGITSFCICLFVLERKEGVAVKWDVLGIILQFITLFSTILFLNQLNNPTISGSSKILLGSMIAVFLGLFIWREKTAESPLLDLSLFSNHKFSAFNLALFFNYMSLYMIIFALPFYIQKVLHYGPFLTGLVLTVSPLLMMIIAPFSGFSSDRIGSRPLAFFGSLISALAFFLMTKLTIFSSIYIVTFLMIVLGVGTAIFQAPNNRAIMASIPNDTSGQASSILVTMRNLGMIFAVSIGSLLISTTIDYNILNSPILYNLESYDFTQGLHLIALLGSGLSLIMAILSLWGSKHLKEVKPLVKDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W4</accession>
    <name>F0T5W4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ApeA_NTD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644708.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1112"/>
    <dbReference type="EnsemblBacteria" id="ADZ09357">
        <property type="protein sequence ID" value="ADZ09357"/>
        <property type="gene ID" value="Metbo_1112"/>
    </dbReference>
    <dbReference type="GeneID" id="10277562"/>
    <dbReference type="KEGG" id="mel:Metbo_1112"/>
    <dbReference type="eggNOG" id="arCOG09518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039923_0_0_2"/>
    <dbReference type="OMA" id="QGLETYH"/>
    <dbReference type="OrthoDB" id="122918at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041223">
        <property type="entry name" value="ApeA_NTD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18862">
        <property type="entry name" value="ApeA_NTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApeA_NTD1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="464" mass="55900" checksum="A4D4BB85F8AFAD70" modified="2011-05-03" version="1">MEKFEERGVFWVPEKNVKLSGVLRFDPQEKIELEIFGSKGETNYPLFSGKIHHEIMLGVISGRNVTLYHCHQIKGQIKDNEFMTLIFDINTIFLGYHFKNKKDICFNSLDIEYSYLEDWVDRTGFNVYPILNSKRYIEEYTTTYKYPQNVHAKLDNFQIQIRFRFQPLQYKYKIDLKQDTYLEIIPNEVIHFKEYKQKILDNIQNFLTLGISEPLFPINITGKIKKNNVKNDDKYPNYTNIEIFYRTITAHFPKKQMNTWDMFFSLEDINFNECLNNWFRKYENLKPVIQLYFGILNNPSHLENNFINLAQAIESYHRRTFQGKYMPTKEYKKLSKILKEKIPEKIAKDHKNSLKSKIDFGNEFSLQTRLYEIFEKYEDVLKININNQDEFIKDVKNTRNFLTHYNKNLEIKAKTKNEDLFDLIQKLKFMLEVCFLFELGINSTKIMKLVSQDKRYQTKYQFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T622</accession>
    <name>F0T622_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645880.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2315"/>
    <dbReference type="EnsemblBacteria" id="ADZ10529">
        <property type="protein sequence ID" value="ADZ10529"/>
        <property type="gene ID" value="Metbo_2315"/>
    </dbReference>
    <dbReference type="GeneID" id="10278781"/>
    <dbReference type="KEGG" id="mel:Metbo_2315"/>
    <dbReference type="eggNOG" id="arCOG02526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_237855_0_0_2"/>
    <dbReference type="OrthoDB" id="101258at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="130"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="230"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="330"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="428"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1810" mass="197072" checksum="300B9A3791DB0E4B" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T637</accession>
    <name>F0T637_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0292 protein Metbo_2331</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0292 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10544">
        <property type="protein sequence ID" value="ADZ10544"/>
        <property type="gene ID" value="Metbo_2331"/>
    </dbReference>
    <dbReference type="GeneID" id="10278797"/>
    <dbReference type="KEGG" id="mel:Metbo_2331"/>
    <dbReference type="eggNOG" id="arCOG01486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140789_0_0_2"/>
    <dbReference type="OMA" id="VMILTDF"/>
    <dbReference type="OrthoDB" id="99443at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01095">
        <property type="entry name" value="UPF0292"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022972">
        <property type="entry name" value="UPF0292"/>
    </dbReference>
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        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16586" checksum="1FF02AB4C4D87FD0" modified="2011-05-03" version="1">MSFKKLSCIIEELKICAEQGMPILIEGKKDENALKKLGINGTFIKVSGSGLKLFEIAEKSVKLSSKVIILTDFDKKGEQLAKRLAGDIQSLGSYPNLEIRRKIMGITRKFIKDIESLPRHMEQLELEEKPDGVSDNFDHQRFCVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T645</accession>
    <name>F0T645_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prf1</name>
        <name evidence="11" type="ordered locus">Metbo_2339</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645903.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2339"/>
    <dbReference type="EnsemblBacteria" id="ADZ10552">
        <property type="protein sequence ID" value="ADZ10552"/>
        <property type="gene ID" value="Metbo_2339"/>
    </dbReference>
    <dbReference type="GeneID" id="10278805"/>
    <dbReference type="KEGG" id="mel:Metbo_2339"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="OMA" id="GQEMEVV"/>
    <dbReference type="OrthoDB" id="32191at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PTHR10113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="SSF55481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03676">
        <property type="entry name" value="aRF1/eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1_1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="409" mass="46199" checksum="B23DDBF8AE0DE08A" modified="2011-05-03" version="1">MSEVSSKELYEVKRTLKELADKKGRGTELVTVYIPPEKQISDVVKHMREELSQSANIKSKQTKKNVQSAIEVIMQRMKLFPKPPEKGLVLFVGMIPRGGPGTEKMETYMFEPPEPVQTYIYHCDSTFYLKPLEEIIEDKDVYGLAVIDRKEATIAILRGKRIDIIKNLTSGVPGKHKAGGQSQRRFDRLIDLAAHEFLKRIGTHMNEAFLDVPDLKGVIIGGPGHTKEEFVEGDYLHHEIKNKLITTVDTSYTGEFGIREVMEKSMDVLTEIDVVREKKLVQKFLVELIDEHGLASYGEAEVRKNLQMGAVEVLLLSEDLKSKRLNYECPTCGTKAEKTLKSDVAIPEKTCSNCNERMKVEETVDIIDDFVELAEEVGSEVEIISTETEEGMQLFRAFGGVGAILRYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C2</accession>
    <name>F0T6C2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644788.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1193"/>
    <dbReference type="EnsemblBacteria" id="ADZ09437">
        <property type="protein sequence ID" value="ADZ09437"/>
        <property type="gene ID" value="Metbo_1193"/>
    </dbReference>
    <dbReference type="GeneID" id="10277643"/>
    <dbReference type="KEGG" id="mel:Metbo_1193"/>
    <dbReference type="eggNOG" id="arCOG01783">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_5_0_2"/>
    <dbReference type="OMA" id="WYFPSIG"/>
    <dbReference type="OrthoDB" id="42003at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030798">
        <property type="term" value="F:trans-aconitate 2-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023149">
        <property type="entry name" value="Trans_acon_MeTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="30339" checksum="93B15210C26B90A1" modified="2011-05-03" version="1">MFKWDAKEYQKSSSAQQKWAKELIKKMDLNGSEKMLDLGCGDGKITSEIATNLEKGCILGIDSSEDMIKLAKETFPETEQPNLKFKVKDFQDLNYNEEFDLIFSNAALHWVKDHSNILKGIKQSLKPNGKILIQMGGKGNGKEILDISTEITHKPEWNSYFQDFSFPYGFYDTNIYEKWLKEAGLKALRVELISKIMDQNGVEGLKSWMQTVWLPYTQRIPVDLQEKFIDEIAIKYLENHPMDKDGMVHVNMVRLEVEAINSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C4</accession>
    <name>F0T6C4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644790.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1195"/>
    <dbReference type="EnsemblBacteria" id="ADZ09439">
        <property type="protein sequence ID" value="ADZ09439"/>
        <property type="gene ID" value="Metbo_1195"/>
    </dbReference>
    <dbReference type="GeneID" id="10277645"/>
    <dbReference type="KEGG" id="mel:Metbo_1195"/>
    <dbReference type="eggNOG" id="arCOG04440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891443_0_0_2"/>
    <dbReference type="OMA" id="NDISGMF"/>
    <dbReference type="OrthoDB" id="107309at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019235">
        <property type="entry name" value="DUF2178_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09946">
        <property type="entry name" value="DUF2178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15196" checksum="E06A89DA6F7F2484" modified="2011-05-03" version="1">MNEKSLKILFILTSMIQSVLWIVGLLFANIWFVLAAIIVVLIILPLVYIHRNDISGMFQGKDIMEDERTELINEKSSTVTLGALVGIILYAGLIIISLRNSYPDIQLAGYTLFATAVLALIINMISRIYYKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E1</accession>
    <name>F0T6E1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="6">HAT</shortName>
            <ecNumber evidence="6">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Homoserine transacetylase</fullName>
            <shortName evidence="6">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">metXA</name>
        <name evidence="9" type="ordered locus">Metbo_1213</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644807.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1213"/>
    <dbReference type="EnsemblBacteria" id="ADZ09456">
        <property type="protein sequence ID" value="ADZ09456"/>
        <property type="gene ID" value="Metbo_1213"/>
    </dbReference>
    <dbReference type="GeneID" id="10277664"/>
    <dbReference type="KEGG" id="mel:Metbo_1213"/>
    <dbReference type="eggNOG" id="arCOG00627">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_1_2"/>
    <dbReference type="OMA" id="TRFCVVS"/>
    <dbReference type="OrthoDB" id="8770at2157"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="375"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="436"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="489" mass="54456" checksum="A63D4BBA1A302587" modified="2011-05-03" version="1">MKNESIGIVETKYHSLSDDLILESGEKLKNVQVAYETYGKLNKEKSNAILVCHALTGDAHVAGWYEGDKKPGWWDVIIGPGKCLDTEKYFIICSNVIGGCKGSTGPSSINPETNKPYGLDFPIITIKDMVNAQKKLVNSMGVTQLFAVIGGSMGGMQVLQWCVSYPDMVRSAIPIATTAFSSPQQIAFNEVGRRAIISDPNWNNGQYYDSEVPTEGLALARMIAHITYLSNESMYQKFGRRLQDKEDYGFDFETDFEVESYLHYQGNSFTKRFDANSYLYITKAVDYFDLAGEGSLAETFYGLKIKFLVISVDSDWLYPPSLSRDIVMGLNANDINVSYCEIKSSYGHDAFLIEAGQLNYLIAGFLSDTLVKDVMAKEFPKIKEKSSIEHAAELMLHEKVTHLPVVTDNSTLLGIVTAWDISKSVARNYKELDDIMTKEVIVVSPEDPIELAARKMKKYSISSLPVVDESETVIGIVTMDHVSSMMTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F5</accession>
    <name>F0T6F5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645940.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2376"/>
    <dbReference type="EnsemblBacteria" id="ADZ10589">
        <property type="protein sequence ID" value="ADZ10589"/>
        <property type="gene ID" value="Metbo_2376"/>
    </dbReference>
    <dbReference type="GeneID" id="10278842"/>
    <dbReference type="KEGG" id="mel:Metbo_2376"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="12"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="456" mass="49992" checksum="06859F10A5E50208" modified="2011-05-03" version="1">MVSSNRSIERYALALSVLISFLVAYVSSAINIALPEIATTFSLNSVYLTWIPTSYLLFSAIFLIPFGKIADIYGKKRIISYGLIIFVLASITSALSVSGNMLLISRIFQAIGSAMIYGNVYSLLASVHSEGEAGKPLSISVASAYVGLSVGPILGGFLTEYLGWRSIFFFTIPITVFAMLFIYKLKGEWFGEDGEIFDIKSTSVLAISLILVMYGFSVITNILGILCLIIGFMGVIMFIWLQKKVINPLIHPKLLFDRVYIINNLTSLVNYGPGIFTIFLLSLYLQDIRMLNPTQTGLLLCVQSVFIAIASLLVAKLLDFTLPRYIAAVGMALTGIGLALFLFLTETTSLIYIMVALSIIGLGYGFSAASSTEISLKYVEKRFYGVSSATLNTMRVLGQMMGMGVTTAVLAIFIGTASLTQSNQIIFIQTSKIPFLIYAILCFVSIYGYFIIRRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J8</accession>
    <name>F0T6J8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2419"/>
    <dbReference type="EnsemblBacteria" id="ADZ10632">
        <property type="protein sequence ID" value="ADZ10632"/>
        <property type="gene ID" value="Metbo_2419"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2419"/>
    <dbReference type="eggNOG" id="arCOG10208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192657_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9341" checksum="427E22694C1CA829" modified="2011-05-03" version="1">MGYYIVGDIKLSTRFAGDTMSKLSDEDKAFEQSLKGKMGWKCSCSLDIVDKKCSLKEVVCKGCGKTFKTNSDANYCFDCAKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P3</accession>
    <name>F0T6P3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-6y C5,15-methyltransferase (Decarboxylating), CbiE subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643627.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0023"/>
    <dbReference type="EnsemblBacteria" id="ADZ08276">
        <property type="protein sequence ID" value="ADZ08276"/>
        <property type="gene ID" value="Metbo_0023"/>
    </dbReference>
    <dbReference type="GeneID" id="10276445"/>
    <dbReference type="KEGG" id="mel:Metbo_0023"/>
    <dbReference type="eggNOG" id="arCOG00650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_2_0_2"/>
    <dbReference type="OMA" id="DPCFSGL"/>
    <dbReference type="OrthoDB" id="90646at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="22469" checksum="7361DE64874D5877" modified="2011-05-03" version="1">MSNLYLVGIGPGSGKYLTKEAEDVVNSSEIVIGSKRALKLFPDMKAEKIELNAQNMGEMLKLAVSKAADNNKVALLSTGDPGFSGLLKPIQKIKGNLDLEVVPGISSIQLCASKLQIPWDEANIITMHGKGFSSSLLPLLSNGRPTIILPNTTINETVNYLMEKGIEPNRAVAVCENLSYENEKVVKAKLEELLAEKFGYMCVLVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R4</accession>
    <name>F0T6R4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-phosphoserine--tRNA(Cys) ligase</fullName>
            <shortName evidence="1">O-phosphoserine--tRNA ligase</shortName>
            <ecNumber evidence="1">6.1.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical O-phosphoseryl-tRNA(Cys) synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-phosphoseryl-tRNA(Cys) synthetase</fullName>
            <shortName evidence="1">SepRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sepS</name>
        <name evidence="3" type="ordered locus">Metbo_0044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L-seryl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:25678"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Interacts with SepCysS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.27" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643648.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0044"/>
    <dbReference type="EnsemblBacteria" id="ADZ08297">
        <property type="protein sequence ID" value="ADZ08297"/>
        <property type="gene ID" value="Metbo_0044"/>
    </dbReference>
    <dbReference type="GeneID" id="10276467"/>
    <dbReference type="KEGG" id="mel:Metbo_0044"/>
    <dbReference type="eggNOG" id="arCOG00411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_506822_0_0_2"/>
    <dbReference type="OMA" id="RSHMTSG"/>
    <dbReference type="OrthoDB" id="6499at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043816">
        <property type="term" value="F:phosphoserine-tRNA(Cys) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01674">
        <property type="entry name" value="Sep_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005246">
        <property type="entry name" value="O-Pseryl-tRNA(Cys)_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF38">
        <property type="entry name" value="PTHR11538:SF38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00470">
        <property type="entry name" value="sepS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="2">
        <location>
            <begin position="204"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="531" mass="60902" checksum="A6966BB12CA66A2C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S9</accession>
    <name>F0T6S9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0059"/>
    <dbReference type="EnsemblBacteria" id="ADZ08312">
        <property type="protein sequence ID" value="ADZ08312"/>
        <property type="gene ID" value="Metbo_0059"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0059"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_2_0_2"/>
    <dbReference type="OMA" id="VKYYEIT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="2">
        <location>
            <begin position="54"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="155" mass="18133" checksum="D0D72611DD4963A6" modified="2011-05-03" version="1">MEEKGSNPLEDSKSTNHDPNKLENELDCNSELSKLKKYDIKLLKSMKGFGKTLILWIISKERIHGYEIMSKINKVNPAEQEQLQGKPGKIYPILHDLEKAGLIEGTWESHGKRKVKYYEITEEGVQTLERIKKVFKCHRTALLEEFWRDIFDKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T1</accession>
    <name>F0T6T1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643665.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0061"/>
    <dbReference type="EnsemblBacteria" id="ADZ08314">
        <property type="protein sequence ID" value="ADZ08314"/>
        <property type="gene ID" value="Metbo_0061"/>
    </dbReference>
    <dbReference type="GeneID" id="10276484"/>
    <dbReference type="KEGG" id="mel:Metbo_0061"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_2"/>
    <dbReference type="OMA" id="STWYLVP"/>
    <dbReference type="OrthoDB" id="71648at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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            <begin position="196"/>
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            <begin position="253"/>
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            <end position="298"/>
        </location>
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        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="389" mass="42414" checksum="5B9599B865ADB332" modified="2011-05-03" version="1">MVETKKIMWMIKKDLMVLWRHKPRLISLFLFPIIMITLFGYGMGGTINNIPVVVVDQSQGNLSDATLSAIKGNTLYDVKEITTDPNKGLQMVQNGQAKAAIILPTNYDTLNDTSPKSIVVDVDSSDQLAAGTIIPATQGLFTQINQQIAEQKLQSSSISVSSSQVNGTQVAPYQSILNTINVEVNKLYGNISYLNFLAPAIIGMTILFGCVFGMGEIVAGERERGELARLFMTPTSVATVIGGKIISKLVQETGRGIILIVAAIALFGIIINGSMVLTILLLILGALCFIGFGIMVSARVSSQEDFMQTVMPITMPMMFLSGVFYPIETMPWIFQKIAYILPLTYVNDALRAVMLKGSGVGDIWIDIVVLLGFTAIFFYLGVSKFNRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X3</accession>
    <name>F0T6X3_METLA</name>
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        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Archaea</taxon>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1251"/>
    <dbReference type="EnsemblBacteria" id="ADZ09493">
        <property type="protein sequence ID" value="ADZ09493"/>
        <property type="gene ID" value="Metbo_1251"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1251"/>
    <dbReference type="eggNOG" id="arCOG00142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_2"/>
    <dbReference type="OMA" id="FRRRGMY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
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    </feature>
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            <end position="161"/>
        </location>
    </feature>
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        </location>
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        <location>
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            <end position="222"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
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        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
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        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
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        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="513" mass="55940" checksum="4FD3B30FEC293DA6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T711</accession>
    <name>F0T711_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Energy-converting hydrogenase A, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644882.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1289"/>
    <dbReference type="EnsemblBacteria" id="ADZ09531">
        <property type="protein sequence ID" value="ADZ09531"/>
        <property type="gene ID" value="Metbo_1289"/>
    </dbReference>
    <dbReference type="GeneID" id="10277740"/>
    <dbReference type="KEGG" id="mel:Metbo_1289"/>
    <dbReference type="eggNOG" id="arCOG04828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623471_0_0_2"/>
    <dbReference type="OMA" id="FMGNIVC"/>
    <dbReference type="OrthoDB" id="80296at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011314">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaB"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019706">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="17270" checksum="954928F73BF9E1CD" modified="2011-05-03" version="1">MNEILAIAIAAILSWLNFVIIDTWMGLPESPGVKGADVIGRAVKKRGGDLAGGFFQGNIVCSPDASAGTLLASAACFTIGTPEGGLIAAALVYFGNRLCADPGYAGTTGALTITIIIYLASFIGIGPEYFIVGMVLAIVTIQGLHHPLASKLLGKIAKKMGRYTELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T727</accession>
    <name>F0T727_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gatD</name>
        <name evidence="12" type="ordered locus">Metbo_2448</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646012.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2448"/>
    <dbReference type="EnsemblBacteria" id="ADZ10661">
        <property type="protein sequence ID" value="ADZ10661"/>
        <property type="gene ID" value="Metbo_2448"/>
    </dbReference>
    <dbReference type="GeneID" id="10278920"/>
    <dbReference type="KEGG" id="mel:Metbo_2448"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="OMA" id="RKNHTSR"/>
    <dbReference type="OrthoDB" id="61613at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="SSF141300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatD_N" evidence="11">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase" evidence="9">
        <location>
            <begin position="93"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase_C" evidence="10">
        <location>
            <begin position="305"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="437" mass="47772" checksum="48989E8601E5496E" modified="2011-05-03" version="1">MVYKGLSAEFLDSSGLSIGDLIKVEKNGTSYFGILLDRAEDPDEKHIVIKLNSGYNIGIDVNNATAELVESGDKPKIELAPLNIEKDAEKPDISIVSTGGTVASVIDYKTGAVHPAFTAEDLIRANPELLEHANINGKAIFNILSEDMKPEYWVKTARSVADEINNGADGVVIAHGTDTMHYTSAALSFMLKTPVPVVVTGAQRSSDRPSSDAYMNLISSVVAAKSDIAEVSLCMHAEEDDSFCYLHRGTKVRKMHTTRRDTFRSINSLPLAKIQKSKLKLVDKAMVYNKRSTESLIIEDEIEEKVAFIKSYPGIQAELIDYHIDKGYKGMVLEGTGLGHCPEELIPSLERAADESIPVIMTSQCLYGLVNMNVYSTGRRILSAGVISGLDMLPETAFVKLAWALGQTDNIKEVEKIIHTNVAGELGEKSSEKFFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T733</accession>
    <name>F0T733_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646018.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2454"/>
    <dbReference type="EnsemblBacteria" id="ADZ10667">
        <property type="protein sequence ID" value="ADZ10667"/>
        <property type="gene ID" value="Metbo_2454"/>
    </dbReference>
    <dbReference type="GeneID" id="10278926"/>
    <dbReference type="KEGG" id="mel:Metbo_2454"/>
    <dbReference type="eggNOG" id="arCOG00960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_2"/>
    <dbReference type="OMA" id="NERGYHY"/>
    <dbReference type="OrthoDB" id="124457at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11756" checksum="72FB2DC143A24293" modified="2011-05-03" version="1">MTEKKDKNEPYPVINALECKECERCVLACRKGVLKMSDSLNERGYHYVIYTGEGCTGCGDCYYTCPEPLAIEVHIPKKAKKKKEKEEDIEWQHNSLKETQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T742</accession>
    <name>F0T742_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 13">DNA ligase</fullName>
            <ecNumber evidence="13">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">lig</name>
        <name evidence="16" type="ordered locus">Metbo_2463</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646027.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2463"/>
    <dbReference type="EnsemblBacteria" id="ADZ10676">
        <property type="protein sequence ID" value="ADZ10676"/>
        <property type="gene ID" value="Metbo_2463"/>
    </dbReference>
    <dbReference type="GeneID" id="10278935"/>
    <dbReference type="KEGG" id="mel:Metbo_2463"/>
    <dbReference type="eggNOG" id="arCOG01347">
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
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    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="ADZ10691.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="34440" checksum="821A5D147D90673F" modified="2011-05-03" version="1">MDESTKTPSHYLFKSCTAGSIYPGIEISTRYVLDSLELNFINDPRQSSCTGSGVNLGVVPMETNMALNARNLSLAGETDSDVVCICPLSYTNLKHCQKLISKDNKLENNYEKIMDEIGLKYDIKSDVNHISDLFLEYKDDIAGLTQEFSSNIRVATHHGCQYSKFFFKDVSSGTFEHPTVLDEILKGVGFEVVDYDEQFLCCGGGLHRYMADKEYSKKTFRKKFQSLAEVMPDIIVTQCPGCTFNMDYLQENVLEELKMECSIPVLNIAELLALLMGVEPENIGLDMHAVDLEPFIKKLELGRDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T781</accession>
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        <submittedName>
            <fullName evidence="3">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">Metbo_2502</name>
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    <organism evidence="3 4">
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Ivanova N."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="protein sequence ID" value="ADZ10715"/>
        <property type="gene ID" value="Metbo_2502"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_2502"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="36351at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="MvhB-like"/>
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        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005658">
        <property type="entry name" value="FwdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="248"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="334" mass="36463" checksum="B2211323EC7EF64F" modified="2011-05-03" version="1">MSSLERNGDEIRSLKHSNEKCVGCGICSSICPTKSINNGPVLPIARGLVDMDYININKNSCCLCGLCAAACPFDALEFKIDGEDIKKMDEYPKWIKDAAIDSEACIYCKACETACPQDAIRVQRTLPKREDLVIGEIGVDQDKCISCKICEELCPGDAITVQKTGRNEYETVVDEDKCVYCLVCKRACPTDAIKAVCSSCAYQDYKFDPETFKTHGNTILNPDSCVNCGWCQEICPVDAAVVTKPFEGEASFDDEAECKGDSCHACQDVCPCNAIEIVDGKSVIDQRVCVLCGACANVCPQKCIQINRTKINLENVRSKSWNNILSNLTSGTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T782</accession>
    <name>F0T782_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transcriptional regulator, ModE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2503</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ModE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646067.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2503"/>
    <dbReference type="EnsemblBacteria" id="ADZ10716">
        <property type="protein sequence ID" value="ADZ10716"/>
        <property type="gene ID" value="Metbo_2503"/>
    </dbReference>
    <dbReference type="GeneID" id="10278977"/>
    <dbReference type="KEGG" id="mel:Metbo_2503"/>
    <dbReference type="eggNOG" id="arCOG00223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232769_0_0_2"/>
    <dbReference type="OMA" id="RSYAHAQ"/>
    <dbReference type="OrthoDB" id="82570at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Mop" evidence="5">
        <location>
            <begin position="164"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="25970" checksum="0560387492703C05" modified="2011-05-03" version="1">MDRTKNGPQYRLKFDDKIILLNKKKFNLLENIDECGSIMKASKKVNIPYRSALKYIEELENESNKSIVSTQRGGKGGGGESKLTENGKSILKEYRKVESILKMHDDVNEIESNIVDIDQKNKIANIKLNNENVVLPLRGNFEVGDKVLVLISPEDVFVMLEPQESSVRNIFPGKIIGMELKNHLVRLNVDTGEITLFVDVTEYSREKLNLTLGKDIYIGFKAAAIAMVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F5</accession>
    <name>F0T7F5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="4">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">ImGP synthase subunit HisF</fullName>
            <shortName evidence="4">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hisF</name>
        <name evidence="6" type="ordered locus">Metbo_0131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643735.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0131"/>
    <dbReference type="EnsemblBacteria" id="ADZ08384">
        <property type="protein sequence ID" value="ADZ08384"/>
        <property type="gene ID" value="Metbo_0131"/>
    </dbReference>
    <dbReference type="GeneID" id="10276556"/>
    <dbReference type="KEGG" id="mel:Metbo_0131"/>
    <dbReference type="eggNOG" id="arCOG00617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="OrthoDB" id="57066at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30181" checksum="B9916F5131AD1F89" modified="2011-05-03" version="1">MLAKRIIPCLDCDLNVPHGRVVKGVEFKEIRYAGDPVELATRYYEDGADEIVFLDITASHERRETMADVIKATTENVFVPICVGGGIRKPDDYVNMLRAGADKCSTNTAAIHNPDLINEASKIVGSQACVIGIDAKRRYVSDPSEKPEKIVVETKKGYCWFDCSIYGGREFTGIDALAWAIECQERGAGEILLTSMDRDGTKDGYDLELTRTMSENLDIPVIASGGVGNPQHIYEAFEIGKADAALAASIFHFNEYPVPEVKKYLVDKGIPVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G6</accession>
    <name>F0T7G6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643746.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0143"/>
    <dbReference type="EnsemblBacteria" id="ADZ08395">
        <property type="protein sequence ID" value="ADZ08395"/>
        <property type="gene ID" value="Metbo_0143"/>
    </dbReference>
    <dbReference type="GeneID" id="10276569"/>
    <dbReference type="KEGG" id="mel:Metbo_0143"/>
    <dbReference type="eggNOG" id="arCOG02069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167315_0_0_2"/>
    <dbReference type="OrthoDB" id="102151at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="120" mass="13280" checksum="96FF032596266518" modified="2011-05-03" version="1">MESILIVVDKAPYGYEDTFSAFYVSIACLNRGMEADVLLIGDGVYAAIKGQKPGGTVDYPNVGELSYLIFPESKIFVHSSSLERRSIEEGELVEAAEIIDDIQLSQILLSKTKNRAFMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J4</accession>
    <name>F0T7J4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644913.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1320"/>
    <dbReference type="EnsemblBacteria" id="ADZ09562">
        <property type="protein sequence ID" value="ADZ09562"/>
        <property type="gene ID" value="Metbo_1320"/>
    </dbReference>
    <dbReference type="GeneID" id="24964548"/>
    <dbReference type="KEGG" id="mel:Metbo_1320"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447695_0_0_2"/>
    <dbReference type="OrthoDB" id="94468at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10018" checksum="0CC36D85EFDF0B2D" modified="2011-05-03" version="1">MVGGLIGLEEAEFRLPFLLQRFSKTAKNAVALNMLISLITVISTYYFRLNNFDMSLIYPQIFLMVAIIIGSTTGAYYGIGCPKKYRINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q4</accession>
    <name>F0T7Q4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2523"/>
    <dbReference type="EnsemblBacteria" id="ADZ10736">
        <property type="protein sequence ID" value="ADZ10736"/>
        <property type="gene ID" value="Metbo_2523"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2523"/>
    <dbReference type="eggNOG" id="arCOG00225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971843_0_0_2"/>
    <dbReference type="OMA" id="AHRIVWK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="292" mass="32598" checksum="0C6DECB34E516176" modified="2011-05-03" version="1">MKLYVIIMNYDPKLGMVINGQEFSYKIFETLECIAKTYSQREAAKKLGISHSVLNRRIKESEEKINSKLVETTGAGSGLTENGHDLLNKYKSLMKRLEERKKPVICGGFVSTGLMEALIEEYDLNATVLRTNDMSALELSDKDMVDILALDDPVTAFIYDLDIIPVAYDHLVLVGDSAVNDISDLNGKDFVEIPNSSQRLAWNTMDNMGIDYNIVQTARSPQTALNAVKNNSELFTFLNNSFTEGSDVIKNETKHIVGVVLLNKTNSSLKNFVKFVVGEAHQTIENNGFSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q9</accession>
    <name>F0T7Q9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646092.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2528"/>
    <dbReference type="EnsemblBacteria" id="ADZ10741">
        <property type="protein sequence ID" value="ADZ10741"/>
        <property type="gene ID" value="Metbo_2528"/>
    </dbReference>
    <dbReference type="GeneID" id="10279002"/>
    <dbReference type="KEGG" id="mel:Metbo_2528"/>
    <dbReference type="eggNOG" id="arCOG10131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046970_0_0_2"/>
    <dbReference type="OMA" id="NVNDHRS"/>
    <dbReference type="OrthoDB" id="22071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="41924" checksum="8DFCE3255576A53A" modified="2011-05-03" version="1">MVQNNKIVQSDDMCACSIEGLLEEKKDENRLCSSNPNDVEVFVDPDPEKISQKFYLKRMTDGLPIIPPTKERVLKLLKYTDLDPNETVTVLPPRMGIATPEKIAINSVMAGCLPQFMPVVLNCVKSLSCEKFNTAGINATTHPVAICTIMNGPLSKEIGAGSGAGCLGPGNLANATIGRALRLCMINIAGAVPGVGDHATMGSPAKYSYAFGENETENPWDSLSVERGHDPSVSTTTTMAVEAPQNVNDHRSQTAEDLLDTIAKTASTPGSNNSHVPGEFLLIMSPEHAKTVYKHGFSKQDVKEYLHEHCIVDVELADRGGRKIEPEWIEGDHVHLTRSPEDIVLVVAGGVGRHSMLCHGFGTSSESVTLPIMLKKGIIPKSVEDFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X1</accession>
    <name>F0T7X1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643809.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0206"/>
    <dbReference type="EnsemblBacteria" id="ADZ08458">
        <property type="protein sequence ID" value="ADZ08458"/>
        <property type="gene ID" value="Metbo_0206"/>
    </dbReference>
    <dbReference type="GeneID" id="10276632"/>
    <dbReference type="KEGG" id="mel:Metbo_0206"/>
    <dbReference type="eggNOG" id="arCOG00551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067260_0_0_2"/>
    <dbReference type="OMA" id="AVMNVQR"/>
    <dbReference type="OrthoDB" id="74309at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="34140" checksum="4DAEDC6F77148B2F" modified="2011-05-03" version="1">MDEFFQKLREIQKKERSISGLSRVGDNFYTEVSNYLNGLMRKIDDNPFSFESYLLRDAQRIVAEICERREHKIANSAVMNVQRAYQLFKESKAKGSSDNQITVPINSTPEEEFLYLELVESIVKYRGKLTAPLMSYIQKNHGNNGKMPVKSKSRPERSAKSLDIDSLISDVSGVPQPVEDDVKDIPDGDFESQIIERYGSEPSRGAKNKSDDSESVEDKAETPKKNIDSEQYSSEAEEEKPSEKLGSTFIKTLMILDELPSIVGVDKIVYGPVSPQDLITIPEPNAKILVKNKKGIFIQTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T875</accession>
    <name>F0T875_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643838.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0235"/>
    <dbReference type="EnsemblBacteria" id="ADZ08487">
        <property type="protein sequence ID" value="ADZ08487"/>
        <property type="gene ID" value="Metbo_0235"/>
    </dbReference>
    <dbReference type="GeneID" id="10276665"/>
    <dbReference type="KEGG" id="mel:Metbo_0235"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142216_0_0_2"/>
    <dbReference type="OMA" id="YIGYCIS"/>
    <dbReference type="OrthoDB" id="98686at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17695" checksum="3C4B4C897153A92D" modified="2011-05-03" version="1">MITYDETDEKDLELISDMWKKLIHHLQSQSTYFPMDYQCLFFEKRKEQFENIAESGNLRLDIVKDGENYLGYSVSSIVGEKGSVESLYVDKRCRKEGIGNKLMERALDWMKLNEVSDLEILVSYGNEDALKFYEKYEFYPKHLILKKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B7</accession>
    <name>F0T8B7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643880.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0277"/>
    <dbReference type="EnsemblBacteria" id="ADZ08529">
        <property type="protein sequence ID" value="ADZ08529"/>
        <property type="gene ID" value="Metbo_0277"/>
    </dbReference>
    <dbReference type="GeneID" id="10276709"/>
    <dbReference type="KEGG" id="mel:Metbo_0277"/>
    <dbReference type="eggNOG" id="arCOG08232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381412_0_0_2"/>
    <dbReference type="OMA" id="KECTACI"/>
    <dbReference type="OrthoDB" id="103377at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="21769" checksum="28060A56BAA30B28" modified="2011-05-03" version="1">MGIKLRILTVWIPKFLIKRELNGTNNITNNYLDQLIVEQGGAPPKQEVLKGNLADRRKMMAMGHNKRVKLLMDLLGETTAMEEGSKRMFEAGLILGQNARKGLGVGNGVEDTIKAAKILYKILGIEFSTENTDGNIILWVSSCALSKYYTPQTCSILSYADKGVLKGLNKNMDLKFVEKITLGSKRCKACINMEGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B8</accession>
    <name>F0T8B8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643881.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0278"/>
    <dbReference type="EnsemblBacteria" id="ADZ08530">
        <property type="protein sequence ID" value="ADZ08530"/>
        <property type="gene ID" value="Metbo_0278"/>
    </dbReference>
    <dbReference type="GeneID" id="10276710"/>
    <dbReference type="KEGG" id="mel:Metbo_0278"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_308743_0_0_2"/>
    <dbReference type="OMA" id="KTFNTIY"/>
    <dbReference type="OrthoDB" id="1590at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="Far_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_4" evidence="2">
        <location>
            <begin position="7"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="395"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="935" mass="106404" checksum="C0B474350A2859A1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C9</accession>
    <name>F0T8C9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643892.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0289"/>
    <dbReference type="EnsemblBacteria" id="ADZ08541">
        <property type="protein sequence ID" value="ADZ08541"/>
        <property type="gene ID" value="Metbo_0289"/>
    </dbReference>
    <dbReference type="GeneID" id="10276721"/>
    <dbReference type="KEGG" id="mel:Metbo_0289"/>
    <dbReference type="eggNOG" id="arCOG01831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072733_0_0_2"/>
    <dbReference type="OMA" id="FTHTYAG"/>
    <dbReference type="OrthoDB" id="40897at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="38953" checksum="E82F51FCB66B0DAD" modified="2011-05-03" version="1">MRARPRDFIYTTDDLFFATTTYLHPHDRILSFLRYIPDLNGDRSLNSKKYIKVDSKKAYDFLGTNYPDYLFDCDITGVQMMGVPNEKIENVLQPTERLNEIMDGEERNPLLEKVVKLADTFHDHTGLSYNKMGVSGSILPGLYIENASDIDFVIYGLKNHRMIMKSFAEIKEANGVLKAVGEDYWQKLYKKRIVDSSLSYDEFRWYESRKHNRGLIDGTLFDILQTRDWDEISGTFGKTRYSPMGTIEIECTVSDAIAAYDNPAVYKVEDVTVLNGLEVPISEVASYTHTYSGQAVEGERVVARGKLEKVMNETTSYRLIVGTTRESVGEYIKLKNLRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L4</accession>
    <name>F0T8L4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ordered locus">Metbo_0304</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643907.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0304"/>
    <dbReference type="EnsemblBacteria" id="ADZ08556">
        <property type="protein sequence ID" value="ADZ08556"/>
        <property type="gene ID" value="Metbo_0304"/>
    </dbReference>
    <dbReference type="GeneID" id="10276736"/>
    <dbReference type="KEGG" id="mel:Metbo_0304"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="OrthoDB" id="56027at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="5">
        <location>
            <begin position="169"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="174"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="41667" checksum="975849501D14799A" modified="2011-05-03" version="1">MEIVLCVTGSIAATECVKLARELKRQGFDVKCFMSEDACNIIHPNAMEFATGQKVVTELTGDIEHVKYAQTDLILVAPATANIISKFAYKMADNPISTLLITGFGYQTPTVFVPSMHESMYRAVSQNIDGLKEEGILFLEPKREEGKAKFPDINDIVLQALRETSEGKLRGKKVLISAGGTYEAIDSVRGITNMSSGKMGLEIAREAFIQGADVTMVNGRVDVPIPSLFERFDVTSASEMINKLTDLLPENDIFISAAAVSDFKLECSDSQKISSDDDLIIHFKPGPKILNMVKELNPNIFLVGFKAESNISEEELVISARKQIEKSGADFVVANDISIEGSGFGSEKNQVVIVDDDTSSVPLCSKQEVAKKIVEKIIERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M7</accession>
    <name>F0T8M7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0317"/>
    <dbReference type="EnsemblBacteria" id="ADZ08569">
        <property type="protein sequence ID" value="ADZ08569"/>
        <property type="gene ID" value="Metbo_0317"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0317"/>
    <dbReference type="eggNOG" id="arCOG01624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_3_2_2"/>
    <dbReference type="OMA" id="GFGAMHL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="383" mass="43784" checksum="3C39FC111D876C97" modified="2011-05-03" version="1">MVKQMLYRELGVTGERLSILGLGCMRLPVKNGDSGQVDMDLAVPLIRTAIDSGINYLDTGYPYHNGQSEIAISKAVKDGYREKVFIADKLPIWLVENRQDMDKYLDEQLKRLGIECIDFYLLHTVKESYWEKMISNGVLEFLDDAKASGKIKYTGFSYHGELELFFDVVDSYNWDMCQVQYNIVDQNYQAGREGIRYAAANGVGVVVMEPLRGGTLTKNVPEEVQEIWDESRVKRKPAEWALRFVWDLDDVDVVLSGMNTLEELKQNLETADQGYPNSLSSEEKEIIREVKSVYSQRKQYNCTQCGYCVPCPQGVDIPANLLQLNNAYMFQDEDNAKMNYYMGVKEEERAVNCTGCGECEKICPQMVPIQKALMDVRKKFEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M8</accession>
    <name>F0T8M8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, beta subunit</fullName>
            <ecNumber evidence="2">4.1.1.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.79" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643921.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0318"/>
    <dbReference type="EnsemblBacteria" id="ADZ08570">
        <property type="protein sequence ID" value="ADZ08570"/>
        <property type="gene ID" value="Metbo_0318"/>
    </dbReference>
    <dbReference type="GeneID" id="10276755"/>
    <dbReference type="KEGG" id="mel:Metbo_0318"/>
    <dbReference type="eggNOG" id="arCOG01614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117492_1_0_2"/>
    <dbReference type="OMA" id="GSVLMNM"/>
    <dbReference type="OrthoDB" id="84988at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03372">
        <property type="entry name" value="TPP_ComE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022494">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03846">
        <property type="entry name" value="sulfopy_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="35"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="20105" checksum="CEE4B91E6FAD3E85" modified="2011-05-03" version="1">MKRIDAIEKLSYELTDELVVCNIGFPSRELHHLKDSPNQFYMMGSMGLASSIGLGVAMSTTKRVVVIDGDGSVLMNMGSMVTVYNQSPKNLVWVVLDNECYGSTGSQCTYASSFELGDVARSIGFKNVFSFDLNSEFDFSEALSSEGPVFVHIKVEPGNADVPVIPMEPEEIRDRFVQTIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N2</accession>
    <name>F0T8N2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KH-domain/beta-lactamase-domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0322</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643925.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0322"/>
    <dbReference type="EnsemblBacteria" id="ADZ08574">
        <property type="protein sequence ID" value="ADZ08574"/>
        <property type="gene ID" value="Metbo_0322"/>
    </dbReference>
    <dbReference type="GeneID" id="10276759"/>
    <dbReference type="KEGG" id="mel:Metbo_0322"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OMA" id="YLDGMIW"/>
    <dbReference type="OrthoDB" id="5321at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="KH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="95"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="191"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="420"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="634" mass="72244" checksum="4FA325503BECD442" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N8</accession>
    <name>F0T8N8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP921964</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643931.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0328"/>
    <dbReference type="EnsemblBacteria" id="ADZ08580">
        <property type="protein sequence ID" value="ADZ08580"/>
        <property type="gene ID" value="Metbo_0328"/>
    </dbReference>
    <dbReference type="GeneID" id="10276765"/>
    <dbReference type="KEGG" id="mel:Metbo_0328"/>
    <dbReference type="eggNOG" id="arCOG04873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143947_0_0_2"/>
    <dbReference type="OMA" id="IGCDCDY"/>
    <dbReference type="OrthoDB" id="86485at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014515">
        <property type="entry name" value="UCP921964"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09893">
        <property type="entry name" value="DUF2120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021964">
        <property type="entry name" value="UCP921964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="15945" checksum="4FAABC86DB0B9BCC" modified="2011-05-03" version="1">MPTHEIAGKVMNELEAFHGSKPAIDTPQILIVRGMSRKRIDPEELSSIISGTMKGLGARTIDVFSEEATDIIGIMDENIRSTVEIPGETDVYGIYRLKESFESMNCHTEYSLGIIGKLAVFLVLWKDKSGMGPLFVELVVSNAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q6</accession>
    <name>F0T8Q6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643949.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0346"/>
    <dbReference type="EnsemblBacteria" id="ADZ08598">
        <property type="protein sequence ID" value="ADZ08598"/>
        <property type="gene ID" value="Metbo_0346"/>
    </dbReference>
    <dbReference type="GeneID" id="10276783"/>
    <dbReference type="KEGG" id="mel:Metbo_0346"/>
    <dbReference type="eggNOG" id="arCOG03226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890295_0_0_2"/>
    <dbReference type="OMA" id="AKPNMIG"/>
    <dbReference type="OrthoDB" id="33494at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011312">
        <property type="entry name" value="UCP019164_menthan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019164">
        <property type="entry name" value="UCP019164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03274">
        <property type="entry name" value="methan_mark_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="303" mass="33905" checksum="3B856EE48820DA3F" modified="2011-05-03" version="1">MYETLTYTGGIHKHEEMTELIEDLGGFVLQENISQMDLVLTLAVPIEDIDKIEDKAKELLGSIKIAPMAGTEIAVVSPTLARQHLPHSACDISEYLRRYGAKDNMIGLSRGAGKGISRISEDEKKLIEEHDLAVFALGSFRECITNKTHLFEDIEIPVVVTGAPDIDLDDIPGADAYVSGLGRIPRRLKRGENIRALKKLVEVIETIFDEKRREILLDPPIVPPVLVKREIENQVEAIAEVYSPLPVVSQLMGVRVKLDYDEYHQEIEDVMVSDQRLGDISEVVKSKMYGYTLVRLLSETSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S8</accession>
    <name>F0T8S8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methyl-viologen-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0368</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643971.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0368"/>
    <dbReference type="EnsemblBacteria" id="ADZ08620">
        <property type="protein sequence ID" value="ADZ08620"/>
        <property type="gene ID" value="Metbo_0368"/>
    </dbReference>
    <dbReference type="GeneID" id="10276805"/>
    <dbReference type="KEGG" id="mel:Metbo_0368"/>
    <dbReference type="eggNOG" id="arCOG02475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095272_2_0_2"/>
    <dbReference type="OMA" id="KIVMFCC"/>
    <dbReference type="OrthoDB" id="91498at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FlpD" evidence="7">
        <location>
            <begin position="8"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="15955" checksum="ABC319AC57903A57" modified="2011-05-03" version="1">MAEDDVKIVMFCCNWCSYGGADTAGTARMQYPPNVRVIRVMCSGRIEPQFVFKAFREGADGVIVAGCHHGDCHYDAGNYKLDRRMRLIYKLAEDLGIGRERIYHDWISASEGEKFANTVTMMVNRIKGLGKSPLKEQLSVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z1</accession>
    <name>F0T8Z1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645170.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1592"/>
    <dbReference type="EnsemblBacteria" id="ADZ09819">
        <property type="protein sequence ID" value="ADZ09819"/>
        <property type="gene ID" value="Metbo_1592"/>
    </dbReference>
    <dbReference type="GeneID" id="10278049"/>
    <dbReference type="KEGG" id="mel:Metbo_1592"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_11_2"/>
    <dbReference type="OMA" id="EMIVAMG"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13104" checksum="B2BD86472C979FF0" modified="2011-05-03" version="1">MDILVAELEERTVNELKNILPKLGHTIVAVVSSGEEAVQKTVELGPDLVLIDLDLLGAMKGVDAGRKIMEYLAVPVIFITVFTKNCLTMSLQLPEDALTLSKPIKEDHLKYAISNLFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z5</accession>
    <name>F0T8Z5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1596</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645174.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1596"/>
    <dbReference type="EnsemblBacteria" id="ADZ09823">
        <property type="protein sequence ID" value="ADZ09823"/>
        <property type="gene ID" value="Metbo_1596"/>
    </dbReference>
    <dbReference type="GeneID" id="10278053"/>
    <dbReference type="KEGG" id="mel:Metbo_1596"/>
    <dbReference type="eggNOG" id="arCOG01961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008635_6_2_2"/>
    <dbReference type="OMA" id="YGFIAVF"/>
    <dbReference type="OrthoDB" id="32119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="9"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="400" mass="43109" checksum="9D46185511540F64" modified="2011-05-03" version="1">MIELLFFFVVLLFVSIFSAVIGRLPVSFQMIFILAGMAVGWAVTGYIDVSKPPYSTIIFLVAEIALVLVLFTDASRVGLRALRNNLSTRLLMVGLPLTIIFGVVVAVLLFPSIPWWVGGIIGAVLAPTDAALGQIVVQNIKVPERIRRTIEIESGLNDGGAVPFLLVFIAIGLASEVFRPMGYFVEVAVEQIIFGAVVGLLIGIVGGKIISKARENSWITPEYQRIAYLCLALMTFFVADEIGGSGFIAAFIGGLSLGYVIKDAGDMLIDFSEAEGQLLNLAVFFLLGIVILPIIPLITWQVVVYAVLSLTVIRMIPVALSLIGTKQSLDSILFIGWFGPRGLASIVLALLAISELDVFPGDTTFISVVFVTVLLSVFAHGLSASPLSNLYARRIKRKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T908</accession>
    <name>F0T908_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0378"/>
    <dbReference type="EnsemblBacteria" id="ADZ08630">
        <property type="protein sequence ID" value="ADZ08630"/>
        <property type="gene ID" value="Metbo_0378"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0378"/>
    <dbReference type="eggNOG" id="arCOG00587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="OMA" id="GCVCPAS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="28024" checksum="2262EB81A2235DEE" modified="2011-05-03" version="1">MMKIAVTGKGGVGKTTLSGTLACLLSQTYKVFAIDADPDMNLASSLGIHEKITPISSMKELIQERTGATPGSSFGEVFRMNPKISDLPESLSINYDEAGRLKILVMGTVDKGGDGCVCPASVLLKALMRNLILKKDEMVILDMEAGVEHLGRKTAEAVDIMIILVEPGLKSLETAERIKRLANDIGIKKIAAVVNKVSSPEEEIFVKNKLNGLEINVLGTIPRDNRVVEADMEGKALVDYPDSKALASIGNIASKIVEMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T969</accession>
    <name>F0T969_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">V-type ATP synthase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">V-ATPase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpD</name>
        <name evidence="7" type="ordered locus">Metbo_0439</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644042.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0439"/>
    <dbReference type="EnsemblBacteria" id="ADZ08691">
        <property type="protein sequence ID" value="ADZ08691"/>
        <property type="gene ID" value="Metbo_0439"/>
    </dbReference>
    <dbReference type="GeneID" id="10276876"/>
    <dbReference type="KEGG" id="mel:Metbo_0439"/>
    <dbReference type="eggNOG" id="arCOG04101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_2_1_2"/>
    <dbReference type="OMA" id="TQKRANA"/>
    <dbReference type="OrthoDB" id="93236at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="PTHR11671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="213" mass="24452" checksum="6B1CA9DC8C44A94E" modified="2011-05-03" version="1">MAQEMIEGINPTRMELLKLKDREKLAVKGHSLLKEKRNALIMEFFNILERVKGSREDVERTLKEAYEDLTAAQIVMGDLAVKKAALSVNESINIDIDSRSVMGVVVPLVDSEIKERSMVERGYGFLETSAKIDEAASKFEKAIKLIIELGEIEKTIILLAAEIESTKRRVNALEHIIVPRIENTVKYIEMRLEEMERENFVRLKMIKKTMEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A9</accession>
    <name>F0T9A9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645211.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09860">
        <property type="protein sequence ID" value="ADZ09860"/>
        <property type="gene ID" value="Metbo_1634"/>
    </dbReference>
    <dbReference type="GeneID" id="10278091"/>
    <dbReference type="KEGG" id="mel:Metbo_1634"/>
    <dbReference type="HOGENOM" id="CLU_2353234_0_0_2"/>
    <dbReference type="OrthoDB" id="111421at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zinc_ribbon_2" evidence="1">
        <location>
            <begin position="4"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10923" checksum="62ED2CD0A926B0D7" modified="2011-05-03" version="1">MTNCKNCGTEISKKNKFCPECGILTGNIDENSSDRIYDDEIRTNRIENEIKLGQGECCGNCEHSNDWGYECSGKCKLHEIYVSLDQKCKDFSSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E2</accession>
    <name>F0T9E2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-metabolising metallo-beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1667</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645244.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1667"/>
    <dbReference type="EnsemblBacteria" id="ADZ09893">
        <property type="protein sequence ID" value="ADZ09893"/>
        <property type="gene ID" value="Metbo_1667"/>
    </dbReference>
    <dbReference type="GeneID" id="10278124"/>
    <dbReference type="KEGG" id="mel:Metbo_1667"/>
    <dbReference type="eggNOG" id="arCOG00547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031965_0_0_2"/>
    <dbReference type="OMA" id="HGYHPDW"/>
    <dbReference type="OrthoDB" id="12902at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Lactamase_B" evidence="4">
        <location>
            <begin position="86"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="RMMBL" evidence="3">
        <location>
            <begin position="442"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="497" mass="57362" checksum="7D9381185CA9E919" modified="2011-05-03" version="1">MVDIDFYGGVDEIGGNKILVKDGKTSFFLDFGMGFSKANEYLSEFLQPRKSNGILDFVELEFLPYIKGIYREDYLEHVGIKYEPEPSVDGVLLSHSHVDHVAYVHHLREDIPIFLSQESYLILRALEETGASAFSEYLHLKKTFHMGPTKKGDGYKRLKLAVERDIRIVKPYKPFSIGDFSIKSAPVDHSLPGASGYIAVNDEETVVYTGDLRFHGRQPELTNKFVKESKKSRPNTLITEGTRIGDSESLSEFEIEAKAIKEIEDLKDLVVVNFPVRDLDRLVTFYRAAQETGRELVVSLKQAYILNLFEGLGYPKIEDVMVYKPKKGWGLLSDDRYSCVNDEWICSSDLDPKQLMADYKIWEREYINWDNTVTHHDLSQNPEDYIFRCDFFEFKELIDIKPESGTYIKSSTEPFDKQMEIMEEKAETWLNHFNLDIMRGYHASGHASGREILEMVREIKPQRVYPVHTQHVELFDVLEEDGIEVIHPELKKGEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E7</accession>
    <name>F0T9E7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645249.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1672"/>
    <dbReference type="EnsemblBacteria" id="ADZ09898">
        <property type="protein sequence ID" value="ADZ09898"/>
        <property type="gene ID" value="Metbo_1672"/>
    </dbReference>
    <dbReference type="GeneID" id="10278129"/>
    <dbReference type="KEGG" id="mel:Metbo_1672"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582923_0_0_2"/>
    <dbReference type="OMA" id="APMHGYE"/>
    <dbReference type="OrthoDB" id="93342at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="168" mass="19382" checksum="FD40A0497B4891D1" modified="2011-05-03" version="1">MVNSELIVLGLIYFKPSHGYIIKKNVKHYFGNPHYTLNNNVLYNTLKKLEGNGYIKGELVTMEKVNKKVYTITAEGEKHLIELVAKPAEPGIDEFDFKVKAVFFDLIPQESRIAAVKPLYESKLEMLREATEKKDEYGSFMPIIAKKVLDYGIIELKNYVDFLEKLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H8</accession>
    <name>F0T9H8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0474"/>
    <dbReference type="EnsemblBacteria" id="ADZ08726">
        <property type="protein sequence ID" value="ADZ08726"/>
        <property type="gene ID" value="Metbo_0474"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0474"/>
    <dbReference type="eggNOG" id="arCOG06498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340733_0_0_2"/>
    <dbReference type="OMA" id="TIKKSYG"/>
    <dbReference type="OrthoDB" id="105422at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="27626" checksum="C72AB124098BA036" modified="2011-05-03" version="1">MTRIKFKEILEITVIKMSKALDVVTAGIIAGIVAYATAILGIGGTVIGAVLGAILYQVMSHLFKEPLEKMETQNFEARIVYVLPLILIVIVEILYIFAILYLKPGSVFYQLEAATNHNLFRSIGAGLIIMGLYPIIQSEYIKKSYGYIILVVGLIVLLGGFADFNTPLTDFYSVIYAEMGIIISLMVIAAISFVAIAITSEAVKIFKEKKNFNVEKQNVKYGERIDDWLDGNHPKAKSTKKHDKNDDGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P7</accession>
    <name>F0T9P7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator NikR, CopG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1701</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645277.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1701"/>
    <dbReference type="EnsemblBacteria" id="ADZ09926">
        <property type="protein sequence ID" value="ADZ09926"/>
        <property type="gene ID" value="Metbo_1701"/>
    </dbReference>
    <dbReference type="GeneID" id="10278158"/>
    <dbReference type="KEGG" id="mel:Metbo_1701"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_0_1_2"/>
    <dbReference type="OMA" id="YCMELFV"/>
    <dbReference type="OrthoDB" id="95722at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHH_1" evidence="5">
        <location>
            <begin position="4"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="NikR_C" evidence="6">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="127" mass="14737" checksum="656B37E3EEDF81D7" modified="2011-05-03" version="1">MTIISISLSEQLLQEIDQLKDETGFSGRSDVIRASARMLIADNQEKKNLEGNINSILILIHKQKSEDKVTEIKHDFEDIIETQIHSHLKEDKCLELFILEGDAKRMHTLTKMFQTTRKIDYVRLITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P9</accession>
    <name>F0T9P9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1703</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1703"/>
    <dbReference type="EnsemblBacteria" id="ADZ09928">
        <property type="protein sequence ID" value="ADZ09928"/>
        <property type="gene ID" value="Metbo_1703"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1703"/>
    <dbReference type="eggNOG" id="arCOG00201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="OMA" id="GHDHVHP"/>
    <dbReference type="OrthoDB" id="87509at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="248" mass="28078" checksum="424C463CE400C9C1" modified="2011-05-03" version="1">MSVEAVKIRNVDLKYDEHLVLEDICLDINEKDFVAVIGPNGGGKSTLLKLILGILKPDSGEVKVFGRDPLKSRNLMGYLPQHINFDPDFPINVFDTVLTGRYHGLFKGYNTEDREAVLKVLDDMEIRDLKDRQISKLSGGQMQRVFIARAIVREPKLLIMDEPMASIDPEMQSSFYNLMARLKEKMAIVLVSHDVGAVSTHVDKIACLNRKLYYHGDVENSAQGLEEIYHCPIELISHGIPHRILKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T3</accession>
    <name>F0T9T3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09962">
        <property type="protein sequence ID" value="ADZ09962"/>
        <property type="gene ID" value="Metbo_1738"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1738"/>
    <dbReference type="HOGENOM" id="CLU_3302718_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="39" mass="4469" checksum="C188949785338B99" modified="2011-05-03" version="1">MCETEIEFKCVNCGNLYNPKIELECPECYDKGEGIKNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T9</accession>
    <name>F0T9T9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645319.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1745"/>
    <dbReference type="EnsemblBacteria" id="ADZ09968">
        <property type="protein sequence ID" value="ADZ09968"/>
        <property type="gene ID" value="Metbo_1745"/>
    </dbReference>
    <dbReference type="GeneID" id="10278202"/>
    <dbReference type="KEGG" id="mel:Metbo_1745"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_0_2"/>
    <dbReference type="OMA" id="WEEDCSA"/>
    <dbReference type="OrthoDB" id="121018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="183" mass="20637" checksum="E133B019E5FEED22" modified="2011-05-03" version="1">MNQTIETIKNRRSVRNYLPEQVKNDEMKIILEAAVYAPTGHNDQPWHFTVIQNPELINEISRGAKEAMGKTGEKWLENLAQNENYHIFHGAPTVVIVSGKKNATTPHADCSAAVENMLLAAESMNIGSCWIGFAAFYFTNPESLKKFDIPEENDVYFGVTLGYKAKENGKAPKRNRNVVTYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V5</accession>
    <name>F0T9V5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrS</name>
        <name evidence="10" type="ordered locus">Metbo_0526</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644129.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0526"/>
    <dbReference type="EnsemblBacteria" id="ADZ08778">
        <property type="protein sequence ID" value="ADZ08778"/>
        <property type="gene ID" value="Metbo_0526"/>
    </dbReference>
    <dbReference type="GeneID" id="10276971"/>
    <dbReference type="KEGG" id="mel:Metbo_0526"/>
    <dbReference type="eggNOG" id="arCOG01886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_2"/>
    <dbReference type="OMA" id="YIGFEIS"/>
    <dbReference type="OrthoDB" id="59062at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02008">
        <property type="entry name" value="Tyr_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023684">
        <property type="entry name" value="Tyr-tRNA-ligase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="213"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="322" mass="36628" checksum="D324751541563AF6" modified="2011-05-03" version="1">MDIDSKMENIKNGTLEVITEEELKDKLQEDKPVAYIGYEPSGNIHLGHAITVKKMIEIQKAGFHVKILLADLHAYLNGKGTLEEIEEISKYNMRCFRALGLSEKTEFVLGSSYQTREDYTYKVYELALSTTLTRAKRSMAQITRDSADHKVAEVIYPIMQVLDMIFLKVDLALGGMEQRKIHMLARESLPKMGYKAPVCIHTPLLHGTDGSDKMSSSKQNYIAIDDEPEVIKKKIQKSYCPQGVVEANPVIEIAEHFIFTDDDKLLIERPEKFGGNLNLTQEELLEKYSSGDLHPMDLKNSVTNRLIEILEPVREYLKSDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y8</accession>
    <name>F0T9Y8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644162.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08811">
        <property type="protein sequence ID" value="ADZ08811"/>
        <property type="gene ID" value="Metbo_0559"/>
    </dbReference>
    <dbReference type="GeneID" id="10277004"/>
    <dbReference type="KEGG" id="mel:Metbo_0559"/>
    <dbReference type="HOGENOM" id="CLU_1718226_0_0_2"/>
    <dbReference type="OrthoDB" id="139640at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17531" checksum="205E992465EDAF79" modified="2011-05-03" version="1">MMDELMDAEGWIDEEWIEEEEWIEDSWMELEKARGEKVIKCSKRKIKASKNKPAAEQVTLIFEDTDNLDIEETYGIKIIGSKVVVGQHTKNNTAFKEVAAKNINEKRFLEYVAGQIEDVNGPELKLTLAFEKGYSIEITNYVSVEEQKNKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z2</accession>
    <name>F0T9Z2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine--pyruvate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0564</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.6.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644166.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0564"/>
    <dbReference type="EnsemblBacteria" id="ADZ08815">
        <property type="protein sequence ID" value="ADZ08815"/>
        <property type="gene ID" value="Metbo_0564"/>
    </dbReference>
    <dbReference type="GeneID" id="10277009"/>
    <dbReference type="KEGG" id="mel:Metbo_0564"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="OrthoDB" id="20398at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="2">
        <location>
            <begin position="11"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="389" mass="42427" checksum="A60AD3549A08D803" modified="2011-05-03" version="1">MNETLLMIPGPTRVSSRVLKAMSESIVNHRSAVFGEILTETNEMMSEVFQTQNQSYLITGSGTAAMEAALGNVISEGDKVLNIVGGKFGERFMQITETHGGVPVELEVEWGNAANPEDIRYILEEEEEIKAVTMVHNETSTGVENPIEEVGKILKDFDALYVVDTVSSLGGADVQVDNFGIDICVTGSQKCLAAPPGMAAITLNDDAWKVVDKTESKTYYLNLKKYRKSGSKAVPETPYTPSVSLMYAMNEALKIIMEEGLEARIKRHEQAAKATVNGVKAIGLELFASEDASSSTVTAVNMPEGMTDKNFRGTMRNKYRIELAGGQDHLKGNVFRIGHMGNITHREIISTIAAIEMSLKEFGYEVELGTGVAAVQESYMPGNNSLEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA00</accession>
    <name>F0TA00_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0572</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644174.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0572"/>
    <dbReference type="EnsemblBacteria" id="ADZ08823">
        <property type="protein sequence ID" value="ADZ08823"/>
        <property type="gene ID" value="Metbo_0572"/>
    </dbReference>
    <dbReference type="GeneID" id="10277017"/>
    <dbReference type="KEGG" id="mel:Metbo_0572"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_0_2"/>
    <dbReference type="OMA" id="PHNVPFI"/>
    <dbReference type="OrthoDB" id="66605at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="PTHR43029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="7">
        <location>
            <begin position="10"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="409" mass="43213" checksum="4AD672A57F388E87" modified="2011-05-03" version="1">MVVLDSGDTAWMLISTALVILMTIPGVALFYGGLIRRENVLNTIFLSFVSFAIVSIIWFVYGYDLTFGSDMWGFMGSIANPLFGGVIESNTLSTYAPTIPTGLYAIFQMTFAAITVALISGAVVERMKFSAWLAFIPIWATLVYMPVAHWMWGGGWLAQMGALDFAGGTVVHVNCGMAALALVMLLGARKNSNLLPHHLGYSIIGTGLLWFGWFGFNAGSALGATNLAVSAMIVTNISACVGMMTWILMDKLQTGKPTILGALSGAIAGLAAITPAAGYVNVTAAIIIGFVAAICAYFAVSHLKPRFGYDDSLDVFGIHGVCGIVGTIAVGFFATPLINSAIKGGLIAGNPAQIGIQLLAVLVIGAYAFVMTLIIGKVIDKTIGLRVETQDEVQGLDINLHEESGYRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA46</accession>
    <name>F0TA46_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09996">
        <property type="protein sequence ID" value="ADZ09996"/>
        <property type="gene ID" value="Metbo_1773"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1773"/>
    <dbReference type="HOGENOM" id="CLU_3227815_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="5211" checksum="944BA6C078D2D06D" modified="2011-05-03" version="1">MNKRFSEGTKKGRFAEITDPDIFQDHEEVIVFTREEFGWIYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC0</accession>
    <name>F0TAC0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="7">PRA-CH</shortName>
            <ecNumber evidence="7">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisI</name>
        <name evidence="9" type="ordered locus">Metbo_0621</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644223.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0621"/>
    <dbReference type="EnsemblBacteria" id="ADZ08872">
        <property type="protein sequence ID" value="ADZ08872"/>
        <property type="gene ID" value="Metbo_0621"/>
    </dbReference>
    <dbReference type="GeneID" id="10277066"/>
    <dbReference type="KEGG" id="mel:Metbo_0621"/>
    <dbReference type="eggNOG" id="arCOG02676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_0_2"/>
    <dbReference type="OMA" id="TGYRSCF"/>
    <dbReference type="OrthoDB" id="101275at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRA-CH" evidence="8">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="15017" checksum="E571F5BB63560C84" modified="2011-05-03" version="1">MDKLDLNFRHEVGGEKLVIAIAQDHVSSEVLMVAYMNEEALKKTLETGIAHYYSTSRKKLWLKGESSGHTQEVKEVLTDCDEDAVLLKVEQNGGACHEGYYSCFFRKIETDSGELKLVKQKIFEPEQIYGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH3</accession>
    <name>F0TAH3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate butyryltransferase</fullName>
            <ecNumber evidence="3">2.3.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0674</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0674"/>
    <dbReference type="EnsemblBacteria" id="ADZ08925">
        <property type="protein sequence ID" value="ADZ08925"/>
        <property type="gene ID" value="Metbo_0674"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0674"/>
    <dbReference type="eggNOG" id="arCOG00855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056531_0_0_2"/>
    <dbReference type="OMA" id="CIVDGPF"/>
    <dbReference type="OrthoDB" id="54088at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050182">
        <property type="term" value="F:phosphate butyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="78"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="33923" checksum="FA908EDE4CE2E37D" modified="2011-05-03" version="1">MITKFEQVFEKIKEHPKKQIAVAVAHDKTVLEAVHLAEELGITDYILVGNKTKILDISKESNLNIKENKIYDEPNKIKAVAAAVELVKTNRADILMKGFVNTDDFLRGVLHREKGLRTGKIMSHVYVLESSALNRMLFVTDGSVNILPDLETKCSIILNSIYLANIFDIEDPKVAITTAIELANPKMPSTIDAAVLAKMSQRGQFSDKIIDGPLALDNALSPWAAKHKGIGGPVAGKADIIVVPSIEAGNMLCKAHVYLTGGDLAGVVVGASAPIVLTSRADTSQSKLNSIATAVLMVNMERNLSIKFGKVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP1</accession>
    <name>F0TAP1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1893"/>
    <dbReference type="EnsemblBacteria" id="ADZ10113">
        <property type="protein sequence ID" value="ADZ10113"/>
        <property type="gene ID" value="Metbo_1893"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1893"/>
    <dbReference type="eggNOG" id="arCOG02232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="OMA" id="CDAKWIP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="400" mass="42853" checksum="235242D08E4E977D" modified="2011-05-03" version="1">MDMKDVIVVGTGAGGATVGRELIKRGMKVTFIERGPSDTTGNAYKHYDNCDVGVELLKTSCVGGTTLVTAGNAVRTCQEPFKKLGIDLETEFNEIEAEMGVSKLPDSHFGDGTNLIMKTAKELGFNTQKMPKFIEPQECKPCGKCAFGCPRNAKWTSQEYVNDSLNKGAKLVTDTNVTELIIKNNEIKGVKTQDKEFYADHVVLAAGGIETPRILRRSNIEAGNHLFVDSFITVGGLLKDIKFDTEVTMNSLISLGDVILAPHYSEMLVNNLREFDARSSDILGMMVKIGDEPSGKVREDHIQKFSTSKDVGLLAKGAAVAAAILKESGVDPKTLTSTIARGAHPGGTAAIGEVVDKNLETEVSGLFVSDASVFPWAPGAPPVLTIVALAKRLGNYIGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR1</accession>
    <name>F0TAR1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006557, signal transduction</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644288.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0686"/>
    <dbReference type="EnsemblBacteria" id="ADZ08937">
        <property type="protein sequence ID" value="ADZ08937"/>
        <property type="gene ID" value="Metbo_0686"/>
    </dbReference>
    <dbReference type="GeneID" id="10277131"/>
    <dbReference type="KEGG" id="mel:Metbo_0686"/>
    <dbReference type="eggNOG" id="arCOG04902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147797_0_0_2"/>
    <dbReference type="OMA" id="TPICGPY"/>
    <dbReference type="OrthoDB" id="92122at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012029">
        <property type="entry name" value="UCP006557"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09884">
        <property type="entry name" value="DUF2111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006557">
        <property type="entry name" value="UCP006557_sign"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="127" mass="14047" checksum="130D1CF5A571877D" modified="2011-05-03" version="1">MNLTNSSTGEEIVPVAMAIHELVNRLPITMRTQKFGGVRIEEGKVLETDYTGPILEKVLLEGEISNETPQDGPYKGTPVIVVPLIENNEVIAAVGVIDTTKGIYSDIMEMTRRPEQNEPDNTKGEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR8</accession>
    <name>F0TAR8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthine synthase</fullName>
            <ecNumber evidence="6">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dphB</name>
        <name evidence="9" type="ordered locus">Metbo_0693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644295.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0693"/>
    <dbReference type="EnsemblBacteria" id="ADZ08944">
        <property type="protein sequence ID" value="ADZ08944"/>
        <property type="gene ID" value="Metbo_0693"/>
    </dbReference>
    <dbReference type="GeneID" id="10277138"/>
    <dbReference type="KEGG" id="mel:Metbo_0693"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="OMA" id="TAGDPMV"/>
    <dbReference type="OrthoDB" id="102360at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="PTHR10882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6 7">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="29110" checksum="EE962E6086DEA2A4" modified="2011-05-03" version="1">MIYFIGLGLYHETDISLKGLIALKQVDHIYAEFYTAKLFGGNIKNLEELVGQEIQVLTREEVEEGNIPIQKAEVDDVAFLIAGDPLIATTHSEMLIQARKMGIKTNVIHASSILSAAPGIAGLQAYKFGKVTTVPFTEKNYFPHSPYMAIKANMESNLHTLVLLDIRAHEDRYMTANQALEYLLQVESIKNENVTTEDSVAVVVARAGSDEPVVKADKIKNLINQDFGGPLHCLMIPADLHFMEAEALVELADAPKELFEDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW3</accession>
    <name>F0TAW3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP004977</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644340.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0738"/>
    <dbReference type="EnsemblBacteria" id="ADZ08989">
        <property type="protein sequence ID" value="ADZ08989"/>
        <property type="gene ID" value="Metbo_0738"/>
    </dbReference>
    <dbReference type="GeneID" id="10277185"/>
    <dbReference type="KEGG" id="mel:Metbo_0738"/>
    <dbReference type="eggNOG" id="arCOG04416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145183_0_0_2"/>
    <dbReference type="OMA" id="KLIEVTM"/>
    <dbReference type="OrthoDB" id="100319at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012017">
        <property type="entry name" value="UCP004977"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09846">
        <property type="entry name" value="DUF2073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004977">
        <property type="entry name" value="UCP004977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="127" mass="13865" checksum="EBB8CED80437F9F7" modified="2011-05-03" version="1">MDSSDTNALKMDFVSSEALKESSSMEKISMIVDKVKEGDLLVIEGGLTPTEEAELIETTMREIDVENFVGIDIYTLEKDKSSFFGLSKKKAIGITIIGPANVMKTVKKRENFLSMIANLGDSGASLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW5</accession>
    <name>F0TAW5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="3" type="ordered locus">Metbo_0740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644342.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0740"/>
    <dbReference type="EnsemblBacteria" id="ADZ08991">
        <property type="protein sequence ID" value="ADZ08991"/>
        <property type="gene ID" value="Metbo_0740"/>
    </dbReference>
    <dbReference type="GeneID" id="10277187"/>
    <dbReference type="KEGG" id="mel:Metbo_0740"/>
    <dbReference type="eggNOG" id="arCOG01695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="OMA" id="MVYGKRN"/>
    <dbReference type="OrthoDB" id="69351at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="2">
        <location>
            <begin position="457"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="661" mass="75149" checksum="0849E9AC2FA07418" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ0</accession>
    <name>F0TAZ0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein Metbo_1916</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1916"/>
    <dbReference type="EnsemblBacteria" id="ADZ10136">
        <property type="protein sequence ID" value="ADZ10136"/>
        <property type="gene ID" value="Metbo_1916"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1916"/>
    <dbReference type="eggNOG" id="arCOG03215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089549_1_0_2"/>
    <dbReference type="OMA" id="CEFCCCK"/>
    <dbReference type="OrthoDB" id="104709at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="179" mass="20932" checksum="2FE1B7D01BCEA3DE" modified="2011-05-03" version="1">MILKVEDIDDMDELTRVELLEKLKKEAKNIHIKDIMNACNFLFEEGKYVQPSYREKFHNAYLKGFILRIKEVKDDKCTIQQLVDMDELKKSLKLLEEQEIDLLRNHPHDPNFSKIYQIISIYTTFVLEEPIHVVGSEFPGGFKVKFENKTYYCPVKDKQKDNPGAVCGFCIAEQDPDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ3</accession>
    <name>F0TAZ3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645490.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1919"/>
    <dbReference type="EnsemblBacteria" id="ADZ10139">
        <property type="protein sequence ID" value="ADZ10139"/>
        <property type="gene ID" value="Metbo_1919"/>
    </dbReference>
    <dbReference type="GeneID" id="10278377"/>
    <dbReference type="KEGG" id="mel:Metbo_1919"/>
    <dbReference type="eggNOG" id="arCOG06027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137928_0_0_2"/>
    <dbReference type="OMA" id="RRWFGHR"/>
    <dbReference type="OrthoDB" id="101098at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="129" mass="15294" checksum="A7895509AD066AA2" modified="2011-05-03" version="1">MFKLKRIYEEPEEEDGYRVLVDRLWPRGVSKERANLDGWIKDIAPSNELRKWYNHELDKWPEFKTRYLNELNEKKDLLKQLKTMAKSHEKVTLLYSAKDEDHNNAVVLGELLNQPTKTVKTSISRIHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB51</accession>
    <name>F0TB51_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645548.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1977"/>
    <dbReference type="EnsemblBacteria" id="ADZ10197">
        <property type="protein sequence ID" value="ADZ10197"/>
        <property type="gene ID" value="Metbo_1977"/>
    </dbReference>
    <dbReference type="GeneID" id="10278437"/>
    <dbReference type="KEGG" id="mel:Metbo_1977"/>
    <dbReference type="eggNOG" id="arCOG00610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926289_0_0_2"/>
    <dbReference type="OMA" id="PKGGYKA"/>
    <dbReference type="OrthoDB" id="89519at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016436">
        <property type="entry name" value="UCP005063_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="HrcA_DNA-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005063">
        <property type="entry name" value="UCP005063_CBS_MJ1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="176"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="238"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="31852" checksum="548DD56952FA2F80" modified="2011-05-03" version="1">MLTSVQKEILQSLINLYRNADASIKGEEIAEIMNRNPGTIRNQMQSLRSLGLVKGVPGPRGGYKPTIKAYHTLNIQASDNQSLVPIFRDGEKVKDITVSQIEFTSITHPGECEAAIKAVGNIKYLDLGDEIRIGPTPVNKLIVDGIVVGRDDMDNILLLDTTAIRSIPKKTVLEVASTDLIQLDPTDSIRNASKILSDRDIEGAPVVEDGHVIGILTLSDIIRAIGRGDEEQNVSEVMSSKNIITVKQDLMIADAIEIMNKNSIGRVIVVDDDASPIGIVTRTDLLDKIAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB65</accession>
    <name>F0TB65_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644367.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0765"/>
    <dbReference type="EnsemblBacteria" id="ADZ09016">
        <property type="protein sequence ID" value="ADZ09016"/>
        <property type="gene ID" value="Metbo_0765"/>
    </dbReference>
    <dbReference type="GeneID" id="10277213"/>
    <dbReference type="KEGG" id="mel:Metbo_0765"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="OrthoDB" id="119044at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="37"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="22976" checksum="4682A0FA76616373" modified="2011-05-03" version="1">MFKKKRQLEIALQDISPHKNPRVELEQYSTPSVIAADLLWNAMAIGDIEGLKLADLGCGTGIFTIGAALLGANEVVGVDTDSEAVSIAETESSKRNLENTRFVVSEITDFTEEVDTVIQNPPFGAQKSNKTAADVIFLDKALEISPVVYSFHMKKTLDFLLKFVAERNATVSHKFEYKFPIPKIYDFHTRESVEVEVVVLRIEKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB81</accession>
    <name>F0TB81_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl29</name>
        <name evidence="6" type="ordered locus">Metbo_0781</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644383.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0781"/>
    <dbReference type="EnsemblBacteria" id="ADZ09032">
        <property type="protein sequence ID" value="ADZ09032"/>
        <property type="gene ID" value="Metbo_0781"/>
    </dbReference>
    <dbReference type="GeneID" id="10277229"/>
    <dbReference type="KEGG" id="mel:Metbo_0781"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_2_2_2"/>
    <dbReference type="OMA" id="ELRDMTP"/>
    <dbReference type="OrthoDB" id="125807at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7822" checksum="0201DFCDCC45A8C9" modified="2011-05-03" version="1">MVILRSKEIRAMEIDEIQKKLDELKAEHSKNISKSAAAGVYENPGKIKELKRTIARVLTIMNEKQQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB98</accession>
    <name>F0TB98_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secY</name>
        <name evidence="15" type="ordered locus">Metbo_0798</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644400.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0798"/>
    <dbReference type="EnsemblBacteria" id="ADZ09049">
        <property type="protein sequence ID" value="ADZ09049"/>
        <property type="gene ID" value="Metbo_0798"/>
    </dbReference>
    <dbReference type="GeneID" id="10277246"/>
    <dbReference type="KEGG" id="mel:Metbo_0798"/>
    <dbReference type="eggNOG" id="arCOG04169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="OMA" id="KWGIGSG"/>
    <dbReference type="OrthoDB" id="13531at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Plug_translocon" evidence="14">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ09049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="448" mass="47892" checksum="5858EA1F97ED5646" modified="2011-05-03" version="1">MKEALQPIFGLLPQVRSPSYRVPFKEKLKWTGIILILYFILTQVPLFGLSSTAVDQFSSLRAVLAGSFGSIITLGIGPIVTASIVLQLLVGGKILNLDLSQHEDKAFFQGTQKILAIIFTLFEASVLVFTGALPASAAGGQWLLILQITIGGILIIFLDEVVSKWGFGSGVGLFIAAGVSGAIISGALNPLASVTSPGVPQGAIPAFIYSLTAGQPGWYLLLPVIATIVVFLIVVYAESMRVEIPLSYGGVKGARGKYPLKFIYASNMPVILTSALLLNIQLFAAMFQKLGFPILGQVVNGRAINGFAYIMTPPNSLSLLITDPLRVLVYAVIFIGSCILFAILWVELSGIGPKQVSKQLHGMGMQIPGHRSSRAHFEKILKRYIPAITVLGGAFVGLLAFGADLTSALGGGTGVLLTVGILYKLYEEIAQEQLMDMHPMLRKFLGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB9</accession>
    <name>F0TBB9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="10">IPK</shortName>
            <ecNumber evidence="3 10">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Metbo_0819</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="3 10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644421.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
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        <location>
            <begin position="5"/>
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        <ligand>
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        <ligand>
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        <ligand>
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        <location>
            <begin position="183"/>
            <end position="188"/>
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        <ligand>
            <name>ATP</name>
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        </location>
        <ligand>
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        <ligand>
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            <position position="14"/>
        </location>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="29015" checksum="11923CEF5A2F18AD" modified="2011-05-03" version="1">MIILKLGGSVITRKNASEPTLDTENLTRICREISESSYENLIVVHGAGSYGHLYAKEYEIGAEINSKKELERKKQGFSKTQNSVKDLNAMVCEHLQKQGIATVSIQPSSFIKTENKRIIKADLDLIKQYLELGFVPVIYGDVVLDANEKIKMAVVSGDQLIKYLAENLKPELVVLGSDVDGVYNQDPKDNPDAELIKVVRSRKDLLSLDSTKTVDVTGGMGGKIDELLEIAKNGIESEIINANTNNNIKRALNGEKEIGTLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG2</accession>
    <name>F0TBG2_METLA</name>
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        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
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    </protein>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Metbo_2012"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="93" mass="10941" checksum="7213D624F8FED6E5" modified="2011-05-03" version="1">MRKLIWWVFAGTSGGPNRARLVMALKKRPQNAHQLSESLNLNYKTVRHHLKLLEENNIITSSGKNKYGELYFLTTGMEENYDIFEDLWNELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH5</accession>
    <name>F0TBH5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2025</name>
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    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="ADZ10244"/>
        <property type="gene ID" value="Metbo_2025"/>
    </dbReference>
    <dbReference type="GeneID" id="10278486"/>
    <dbReference type="KEGG" id="mel:Metbo_2025"/>
    <dbReference type="eggNOG" id="arCOG07599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204302_0_0_2"/>
    <dbReference type="OMA" id="HIFITRD"/>
    <dbReference type="OrthoDB" id="125808at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026493">
        <property type="entry name" value="Cys-rich_pep"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04165">
        <property type="entry name" value="methano_modCys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7155" checksum="CC21F454BFF7B67F" modified="2011-05-03" version="1">MNLGKLNEKCPKCGCKDKTLKRDLDSEFHAHAKTGSLTCSKCGYVFVSKDDEEQDKKKDKTGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH8</accession>
    <name>F0TBH8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">AL-21</strain>
        </source>
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    </dbReference>
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        <property type="gene ID" value="Metbo_2028"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011434">
        <property type="entry name" value="DUF1535"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07553">
        <property type="entry name" value="Lipoprotein_Ltp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="4">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoprotein_Ltp" evidence="3">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoprotein_Ltp" evidence="3">
        <location>
            <begin position="161"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="21983" checksum="4AEAF22A74270FB8" modified="2011-05-03" version="1">MTKICPKCKTENSDNADFCQNCGTELKGSTDAVNSNETSGNGVIGFWNKQSSRGKLGIGIGGICCIGLILIIAISAFMAPDATTTTTNLNNTPTSPSTPSTDTSTNSSGTSVTAGQEQAAKMAQSYLNTMAFSRSGLIKQLKYEGFTQQEAEYGVDQTNADWNAQAAKMAQSYLDSQSFSRSGLIEQLEFEGFTRQQAEYGVQATGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI1</accession>
    <name>F0TBI1_METLA</name>
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        <submittedName>
            <fullName evidence="6">Amino acid/peptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2031"/>
    <dbReference type="EnsemblBacteria" id="ADZ10250">
        <property type="protein sequence ID" value="ADZ10250"/>
        <property type="gene ID" value="Metbo_2031"/>
    </dbReference>
    <dbReference type="GeneID" id="10278492"/>
    <dbReference type="KEGG" id="mel:Metbo_2031"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004790_0_2_2"/>
    <dbReference type="OrthoDB" id="57252at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:oligopeptide transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Dipep/tripep_permease"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="PTHR11654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00924">
        <property type="entry name" value="yjdL_sub1_fam"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
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            <end position="98"/>
        </location>
    </feature>
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            <end position="206"/>
        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
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            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="5">
        <location>
            <begin position="1"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="471" mass="52402" checksum="3E3D63C25439FABB" modified="2011-05-03" version="1">MFKQHPKGLYLLFTTEMWERFSYYGMRAILSLYMIKALFYSTAFTSSIYGYYTGLVYLTPLIGGYIADRYWGNRKSIITGGFLMAMGQFSLALSSYLYTPQAAGTVNSFFVFNPQTEFFLIGLFLLVFGNGFFKPNISSMVGFLYSENDGRRDSAFTIFYMGINLGALISPLLIGGFADTGDPTNFMYGFLIAGIGMLFGLVVFILGKNRFLVDPEGRSVGVIPNHKYDPEDCAKSEGTLTWVEKQRILVIFILAFFGIFFWAAFEQAGVSLTFLAEQHVDRVVTALNFSIPAAWFQSVNPLAILLFAPIFAALWLKLKDMGKEPSIPLKMAMGLLLLSSGFMLLVFATGSLDHGATSISPLWLIAVYVLFTFGELCISPIGLSMVTKLSPKKFTCLLMGVWFLTSAVANILAGQISTLYPDPSLPTPYLLGMPINSFTSFFMIFVVMSLIAAVLLFLIRKRLETMMHGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP2</accession>
    <name>F0TBP2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0870"/>
    <dbReference type="EnsemblBacteria" id="ADZ09119">
        <property type="protein sequence ID" value="ADZ09119"/>
        <property type="gene ID" value="Metbo_0870"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0870"/>
    <dbReference type="HOGENOM" id="CLU_1987636_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="14058" checksum="9B3A1742729B8CE9" modified="2011-05-03" version="1">MWKYILVVICFIGFIIVGFYIFGYEPTNLILNNGEYSFNKDMNLLNQTGKTDPEALVYINGIPAVVDDDGNFYGMVGINNGLNIINVTAKAPFKSITSNIATVKRTETPHHIDVYYQINNTIQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR5</accession>
    <name>F0TBR5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644493.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0893"/>
    <dbReference type="EnsemblBacteria" id="ADZ09142">
        <property type="protein sequence ID" value="ADZ09142"/>
        <property type="gene ID" value="Metbo_0893"/>
    </dbReference>
    <dbReference type="GeneID" id="10277342"/>
    <dbReference type="KEGG" id="mel:Metbo_0893"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_3_0_2"/>
    <dbReference type="OMA" id="HCQNLYP"/>
    <dbReference type="OrthoDB" id="37404at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015393">
        <property type="entry name" value="DUF1972"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09314">
        <property type="entry name" value="DUF1972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1972" evidence="3">
        <location>
            <begin position="10"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="2">
        <location>
            <begin position="206"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="412" mass="47628" checksum="A8C671FD8A9CF8E1" modified="2011-05-03" version="1">MKFSQKKKSSIAIIGSRGIPNNYGGFECFTENISQKLSKKDYEVYVSCEQPEGVDPPEKFEDVNLFYFPIKHPKSNVLGMIYEIMYDAYSLLYSSLKADQIYMLGYSAAFFFFIPKLFGKTLYLNPDGFEWKRNKFSGMIKLMLKIQERMGVFWADKIIADSMGIKEYYDNKYSKSSKFIAYGVSEIPEIGWNDEYLPGSLRGVIEDSNYWLLVARLEPENNVHTIVESYIKSKTKKPLVIVGNFLNSDYQETINNMIEGMDETKNIVFTGGIYDQKSLNMLRQNCFGYIHGHSVGGTNPSLIEAMVMENILLTHRNQFNEEVCQDSALYFKDSDELATKMDDVDNHPDQYQIMKKLALSRVKQEYSWEKIVAAYDEVFGNEYNNQNRQHSPNKFPLSKGYKSDYNSKNNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT1</accession>
    <name>F0TBT1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Two component regulator three Y domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644509.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0909"/>
    <dbReference type="ESTHER" id="metla-f0tbt1">
        <property type="family name" value="Asp2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09158">
        <property type="protein sequence ID" value="ADZ09158"/>
        <property type="gene ID" value="Metbo_0909"/>
    </dbReference>
    <dbReference type="GeneID" id="10277358"/>
    <dbReference type="KEGG" id="mel:Metbo_0909"/>
    <dbReference type="eggNOG" id="arCOG07139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_582187_0_0_2"/>
    <dbReference type="OrthoDB" id="69240at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022267">
        <property type="entry name" value="Asp2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16929">
        <property type="entry name" value="Asp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="469" mass="54197" checksum="C6AEC64D88382B1F" modified="2011-05-03" version="1">MGIKEYIESNEKTHKKGKNIKYIFCEGKTSNQLIVNFPGFAKAHELPQYNYIRTLKNCNCNRLFILDDYGPRGSYLIGESGDSSIEEGVISLINAICEKYRIKREEVITNGTSKGGFCALYYGIKYNFGYVIAGSPQTKLGDYLCFFPEIADYISGGHEPKDISYLNSLLYNLVEDSDEFPEIYIHVGEGDHHYKNHIIPFFNELDKKNVTYHLELRNYFTHSLIGNYYIEYLLKTLKSIDKSIVDPKTPEIISTSIQSNGNHLEVFCGANGDDLEYSVELYSLNDLVEKTSYQNSPKFMYPISTPGKYHAKIYVKDDTSYNTSLTDEIEIKIEENLRGIENADKSRSSNFLGKKFNSLKSKIGSGHLNFFKSKISADELNPLHELKIEKVSSNSYKFTIQRNQDVESFAWYILLNGERVDTIWYRQENYMEYTFKEPGNYQIKYFVKDENSKLMYLSEPILLDKQDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU1</accession>
    <name>F0TBU1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AAA family ATPase, CDC48 subfamily</fullName>
            <ecNumber evidence="5">3.6.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645634.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2064"/>
    <dbReference type="EnsemblBacteria" id="ADZ10283">
        <property type="protein sequence ID" value="ADZ10283"/>
        <property type="gene ID" value="Metbo_2064"/>
    </dbReference>
    <dbReference type="GeneID" id="10278526"/>
    <dbReference type="KEGG" id="mel:Metbo_2064"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OMA" id="ENFRFAQ"/>
    <dbReference type="OrthoDB" id="2808at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="SSF54585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48_N" evidence="4">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48_2" evidence="3">
        <location>
            <begin position="107"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="236"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="508"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="729" mass="80453" checksum="525A9E6AAEE774A6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW1</accession>
    <name>F0TBW1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 4">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645654.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2084"/>
    <dbReference type="EnsemblBacteria" id="ADZ10303">
        <property type="protein sequence ID" value="ADZ10303"/>
        <property type="gene ID" value="Metbo_2084"/>
    </dbReference>
    <dbReference type="GeneID" id="10278546"/>
    <dbReference type="KEGG" id="mel:Metbo_2084"/>
    <dbReference type="eggNOG" id="arCOG00665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="OMA" id="MHYVRQG"/>
    <dbReference type="OrthoDB" id="78828at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="281" mass="31327" checksum="D068FEB9B9B5CC37" modified="2011-05-03" version="1">MKAVIPAAGLGTRFLPATKAQPKEMLPVFNKPTIQYVVEEAVASGIDDILIITGKGKRSIEDHFDKSFELEYFLKSSGKTERLHEIESISEMADIYYVRQKKQEGLGDAIRCAKKHVDGDAFAVLLGDTIARSKVPCTKQLLDIYNKYEASTIAIEKVPDEKVERYGIIGGKQISEDLYEIQELVEKPKLENAPSNLAITGRYVFAPEIFDHLAHINPGFGGEIQLTDAMKSLTKIYGHIYDGKIYDIGNTVEWLKSSVEIALEDPEVGEDLRSYLTGIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY8</accession>
    <name>F0TBY8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2111"/>
    <dbReference type="EnsemblBacteria" id="ADZ10330">
        <property type="protein sequence ID" value="ADZ10330"/>
        <property type="gene ID" value="Metbo_2111"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2111"/>
    <dbReference type="eggNOG" id="arCOG01750">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_2"/>
    <dbReference type="OMA" id="HDSMGEV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008797">
        <property type="term" value="F:aspartate ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="2">
        <location>
            <begin position="404"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="50372" checksum="A1D2E8AA999E37D8" modified="2011-05-03" version="1">MREEEDFLGKKNLPDSAYYGIQTQRAVENFPVTGRGESKEFILAYVMLKIAAAEANMELDNLDHEQGSAIIKAAQEILGGKLLGEFRVDLFQAGAGTSFNMNVNEVIANRALEILGHSKGDYNYLNPNDHVNRSQSSNDTFPTASHVAIVMEADKLMETLHKLSSSMEDKGKSMISVPKSGRTHLMDAMPITIGDEFIAYSEAIKRALNRIKESRNNLLEIPLGGTATGNGVNTPPLYRETVIKKLSKLTSLPLLPAKNGMEALQSRSIMVAFSNSLAELAVELSRIANDLRLMGSGPTSGLNEIVLPPVQPGSSIMPGKVNPVMAECMNMICFQIMGNNSTVSLAGQAGQFELNVMTPVMISNILETLAMLNNYLPVFQEKCIEGIMANKKTIEDRTGKNPSIATLLSPKIGYSEAAKLSKEALKTDQSIKDLVVMKGLMTMKEADKFFDLDKISKNPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC17</accession>
    <name>F0TC17_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0919</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644519.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0919"/>
    <dbReference type="EnsemblBacteria" id="ADZ09168">
        <property type="protein sequence ID" value="ADZ09168"/>
        <property type="gene ID" value="Metbo_0919"/>
    </dbReference>
    <dbReference type="GeneID" id="10277368"/>
    <dbReference type="KEGG" id="mel:Metbo_0919"/>
    <dbReference type="eggNOG" id="arCOG00664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_9_0_2"/>
    <dbReference type="OMA" id="FTWLDTG"/>
    <dbReference type="OrthoDB" id="61185at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="32785" checksum="E507B24F8F8AF3AA" modified="2011-05-03" version="1">MKGIILAGGSGTRLYPITKAVSKQLLPIYDKPMIYYPLSVLMLAGIREILIISTPTDLPRYKELLGDGTDLGVEFSYRVQDEPRGLADAFIVGEDFIGEESVALVLGDNIFHGHRFSEILKRAASLDKGAVIFGYYVKNPRPFGVVEFDEEGNVLSVEEKPENPKSNYVVPGLYFYDNSVIDIAKNIAPSDRGEIEITSVNDEYLKRKELKVELLGRGMAWLDTGTHIGLLEASNYVEAIQKRQGFYIACLEEISFNNGWIDEDIVLKSAESLEKTDYSKYLVELVKRNGNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC29</accession>
    <name>F0TC29_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644531.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09180">
        <property type="protein sequence ID" value="ADZ09180"/>
        <property type="gene ID" value="Metbo_0933"/>
    </dbReference>
    <dbReference type="GeneID" id="10277382"/>
    <dbReference type="KEGG" id="mel:Metbo_0933"/>
    <dbReference type="eggNOG" id="arCOG10464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712850_0_0_2"/>
    <dbReference type="OMA" id="GYEYPIV"/>
    <dbReference type="OrthoDB" id="141095at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="72" mass="8395" checksum="5C878FFE84E39E52" modified="2011-05-03" version="1">MDVAWVNVQYDKDSEKQYNSISDNSIERISIGSETIKIYYSQESERKETYVKIILINNIAGYEYPIVRGRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC42</accession>
    <name>F0TC42_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644544.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0946"/>
    <dbReference type="EnsemblBacteria" id="ADZ09193">
        <property type="protein sequence ID" value="ADZ09193"/>
        <property type="gene ID" value="Metbo_0946"/>
    </dbReference>
    <dbReference type="GeneID" id="10277395"/>
    <dbReference type="KEGG" id="mel:Metbo_0946"/>
    <dbReference type="eggNOG" id="arCOG07473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183471_0_0_2"/>
    <dbReference type="OMA" id="IWAYAVF"/>
    <dbReference type="OrthoDB" id="129146at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043713">
        <property type="entry name" value="DUF5654"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18898">
        <property type="entry name" value="DUF5654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="87" mass="9334" checksum="D394CA384EA053B8" modified="2011-05-03" version="1">MKEKTEEVKTQVAQTISTLMTVAFGLIAALAWNDAIKAIILQYMPKGSGITGLLIYAVIITIIAVVATIIIARALGKPPVQEVRIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC70</accession>
    <name>F0TC70_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644572.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0974"/>
    <dbReference type="EnsemblBacteria" id="ADZ09221">
        <property type="protein sequence ID" value="ADZ09221"/>
        <property type="gene ID" value="Metbo_0974"/>
    </dbReference>
    <dbReference type="GeneID" id="10277423"/>
    <dbReference type="KEGG" id="mel:Metbo_0974"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753580_0_0_2"/>
    <dbReference type="OMA" id="AINEPFG"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="175"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="367" mass="42887" checksum="B6DF30E69EBBA6F6" modified="2011-05-03" version="1">MDIGIVHPELIYPRGAEKQLCKLSYHLNKMGNNVTIYTFEKNDNYIFDPLLENIEIISLNKKWAVNYLFGFNNVRWNQMIKKLASKIGDHDVINAHNHPAQWISSYTETPTVWMCNEPYYYGGSKLNNYFLDKDRQLSSNVKLTLAFNQRMKDIIREMYPDNKKMELTGSGVDLERKIQHENNEYFDSIFVGPLYPRKRQLDIIKAFGKIKDRIPNSRLHFVGGEVGILSKKYKRTLIQTAAEYQVDIMFYDSISDEELYKLYDTADISVFVPEYEPWGIFPLETILGGITTIISDECGVKEVLPDDSMVVETGNIEELSNKILDVHENMEHYRNWTKKASKLIAEEYSWKAYAKRMENFFKNVITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC91</accession>
    <name>F0TC91_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645709.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10358">
        <property type="protein sequence ID" value="ADZ10358"/>
        <property type="gene ID" value="Metbo_2140"/>
    </dbReference>
    <dbReference type="GeneID" id="10278603"/>
    <dbReference type="KEGG" id="mel:Metbo_2140"/>
    <dbReference type="eggNOG" id="arCOG10208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192657_0_0_2"/>
    <dbReference type="OMA" id="CACQTGI"/>
    <dbReference type="OrthoDB" id="122955at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7441" checksum="860021D7DAC4EEBC" modified="2011-05-03" version="1">MVKEVDEDKAFEQKLKGKMGWKCSCSLDIVDKKCSLKEIACKDCGKVFKTNRDVEYCFDCEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC98</accession>
    <name>F0TC98_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645716.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2149"/>
    <dbReference type="REBASE" id="33855">
        <property type="entry name" value="Msp21MrrP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10365">
        <property type="protein sequence ID" value="ADZ10365"/>
        <property type="gene ID" value="Metbo_2149"/>
    </dbReference>
    <dbReference type="GeneID" id="10278612"/>
    <dbReference type="KEGG" id="mel:Metbo_2149"/>
    <dbReference type="eggNOG" id="arCOG09740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551855_0_0_2"/>
    <dbReference type="OMA" id="HIWTRDH"/>
    <dbReference type="OrthoDB" id="98486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014923">
        <property type="entry name" value="DUF1802"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08819">
        <property type="entry name" value="DUF1802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="170" mass="19813" checksum="40521CC21056EBDB" modified="2011-05-03" version="1">MNSTHKCLKEWNATLEALGHGKQTILVRNYKTNVKEFLLYPTVSYASKDDYLDSFQNKYQDFVKDNALPEKNGDKVLIKYFATLERMVEKPVSRLPSDKFYIWTKDHVKNYMSGKTAYIWILRVNKLKELYWAEPTPGAIKYANLKNEVSLDGMKPVLSDSEFEKTVNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA3</accession>
    <name>F0TCA3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645721.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2155"/>
    <dbReference type="EnsemblBacteria" id="ADZ10370">
        <property type="protein sequence ID" value="ADZ10370"/>
        <property type="gene ID" value="Metbo_2155"/>
    </dbReference>
    <dbReference type="GeneID" id="10278618"/>
    <dbReference type="KEGG" id="mel:Metbo_2155"/>
    <dbReference type="HOGENOM" id="CLU_2820887_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="66" mass="7737" checksum="7A8C2AB5BF181C2D" modified="2011-05-03" version="1">MFKKQINIGIEQKTDLDAIQRQIIENGGKVTLMDLINDAISIFINEYKNKAILKYTPQFYDKKEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC8</accession>
    <name>F0TCC8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2180"/>
    <dbReference type="EnsemblBacteria" id="ADZ10395">
        <property type="protein sequence ID" value="ADZ10395"/>
        <property type="gene ID" value="Metbo_2180"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2180"/>
    <dbReference type="eggNOG" id="arCOG04414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170076_0_1_2"/>
    <dbReference type="OMA" id="RWIKVAM"/>
    <dbReference type="OrthoDB" id="131677at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10611" checksum="DFFDFDF1311B04E0" modified="2011-05-03" version="1">MSSSNVLAGTRTELVFEFEDPEDVEVIMGSIEPEIDGHPSDRTSVSIKKDGNNLRIHIESQDSASFRASLNSYLRWIKLSSEILGLKKIDQNCDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD7</accession>
    <name>F0TCD7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P protein component 3</fullName>
            <shortName evidence="5">RNase P component 3</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnp3</name>
        <name evidence="6" type="ordered locus">Metbo_2190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2190"/>
    <dbReference type="EnsemblBacteria" id="ADZ10404">
        <property type="protein sequence ID" value="ADZ10404"/>
        <property type="gene ID" value="Metbo_2190"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2190"/>
    <dbReference type="eggNOG" id="arCOG00307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074509_0_0_2"/>
    <dbReference type="OMA" id="INRAACE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="PTHR13031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="28214" checksum="4DCA885621ABCC0C" modified="2011-05-03" version="1">MEGNYSTKKNYRENFYDLNISGNKSIVQEAERLGFAGVSLTYNFDDYNSEISKEYDELKYESNIIIRKCLEINCKNPEELKKKVRRSRKKADIVMVRGGDNSINRAACEERNVDILSQPYRNRRDSGMNHVLAKKAAENDVAVEINLSSFLKTNLRYRHRIVSQFRHIMALKRKYGFSVIITSGARSSYDQRKPMDIFALSNCFDMTENESFEALSIIPQHIIDRNDNRNNFVLDGVRTVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH4</accession>
    <name>F0TCH4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1004</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644602.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1004"/>
    <dbReference type="EnsemblBacteria" id="ADZ09251">
        <property type="protein sequence ID" value="ADZ09251"/>
        <property type="gene ID" value="Metbo_1004"/>
    </dbReference>
    <dbReference type="GeneID" id="10277453"/>
    <dbReference type="KEGG" id="mel:Metbo_1004"/>
    <dbReference type="eggNOG" id="arCOG04147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_2_2"/>
    <dbReference type="OMA" id="KWGSFDK"/>
    <dbReference type="OrthoDB" id="74803at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="8">
        <location>
            <begin position="93"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="23877" checksum="07A097056C1A9BE1" modified="2011-05-03" version="1">MVQKNYQLPELPYGYKDLEPYISEEQLKIHHTKHHKAYVDGANAILKKFDNRGSEEFDVKAVSKELTFHVGGYVLHKFFWENMGPADKCGGEPTGTIAEYIKKDFGTFERFKKEFSQAATGVEGSGWAVLTLCRMTNRIFIMQVEKHNVNIIPGFRIMLALDVWEHAYYLDYQNRRPDYVEAFWNLVNWEEVNRKMDVWLSSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK9</accession>
    <name>F0TCK9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644637.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1039"/>
    <dbReference type="EnsemblBacteria" id="ADZ09286">
        <property type="protein sequence ID" value="ADZ09286"/>
        <property type="gene ID" value="Metbo_1039"/>
    </dbReference>
    <dbReference type="GeneID" id="25394722"/>
    <dbReference type="KEGG" id="mel:Metbo_1039"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_58_2"/>
    <dbReference type="OMA" id="KINLMSN"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="360"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="558" mass="63447" checksum="605CBE4FDCFA4518" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL8</accession>
    <name>F0TCL8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADZ09295"/>
        <property type="gene ID" value="Metbo_1048"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1048"/>
    <dbReference type="HOGENOM" id="CLU_3194510_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="45" mass="4903" checksum="CCCFE2D2C87860AC" modified="2011-05-03" version="1">MGFLDAIFTGTAAIVIGFILGIILATVVGFIIYGIVKLRDRNKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM5</accession>
    <name>F0TCM5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1055</name>
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    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1055"/>
    <dbReference type="EnsemblBacteria" id="ADZ09302">
        <property type="protein sequence ID" value="ADZ09302"/>
        <property type="gene ID" value="Metbo_1055"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1055"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="LDLINMF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="432"/>
        </location>
    </feature>
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            <end position="463"/>
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    </feature>
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        <location>
            <begin position="28"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="473" mass="51128" checksum="3C5A4C5CB9E2FB28" modified="2011-05-03" version="1">MVGKNQNKKRFILKDQMIHMELNGNNKVLVLLFIGVFMGSLDIGIVGPALPAVQSYFAVNERLLSWVFTIYILFFMIGTPLMAKLSDIYGRKTIYMLDIILFAIGSLITITSISFEMLLIGRAVQGVGAGGIFPVANAFIGDIFPPEKRGGALGIISSVWGISSVLGPILGGLLLKFGWQSLFIINLPISAMLLVGSYYLLPKTVVNRNIKFDWYGLAILSILVISLAYGLNQIEANNFINSLLSLSVLPYLILSLILLPVLWKVEKWIEDPLIQIDLFKSREVKLINTMMVGTGLIQAATIFIPAFVVIGLSLNSSAASLMLLPIVVTMAIGAPIIGKLLDKFGSKNIMITGSFIMSIGLLMLGMFPESLYLIIISGFLVGVGMSTAIGSPPRYIMLVESPAKERASGQALINIITSVGQLMGGALIGALIGSYAGTILGYQLSYFIMGVIALIMTILAFGLKSKKQQMNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP4</accession>
    <name>F0TCP4_METLA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645785.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2220"/>
    <dbReference type="EnsemblBacteria" id="ADZ10434">
        <property type="protein sequence ID" value="ADZ10434"/>
        <property type="gene ID" value="Metbo_2220"/>
    </dbReference>
    <dbReference type="GeneID" id="10278686"/>
    <dbReference type="KEGG" id="mel:Metbo_2220"/>
    <dbReference type="eggNOG" id="arCOG04842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145818_0_0_2"/>
    <dbReference type="OMA" id="YGGMKIR"/>
    <dbReference type="OrthoDB" id="107998at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="14204" checksum="9EDB1D77821ABDE7" modified="2011-05-03" version="1">MALILIRAEDQSKLLNTLADIERHAGLKISGKPRIMDSKKADNIATKILKQKIRSRSAIAVLVKVVDDTTKSIVHIRKIHPPAHVVVISEEYAEFKELEANFNKLSPLRGYYSHKSEKSSDSKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR9</accession>
    <name>F0TCR9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645810.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10459">
        <property type="protein sequence ID" value="ADZ10459"/>
        <property type="gene ID" value="Metbo_2245"/>
    </dbReference>
    <dbReference type="GeneID" id="10278711"/>
    <dbReference type="KEGG" id="mel:Metbo_2245"/>
    <dbReference type="HOGENOM" id="CLU_2581444_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="9056" checksum="C3DAA91D69512950" modified="2011-05-03" version="1" precursor="true">MKRNISKLVMMLVLTVLVASPVAFATTLHTHLDSIAPITYNQGDKISVTAQLWTENCVDGLWDNPMNHYDVYFHLYSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT5</accession>
    <name>F0TCT5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645826.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2261"/>
    <dbReference type="EnsemblBacteria" id="ADZ10475">
        <property type="protein sequence ID" value="ADZ10475"/>
        <property type="gene ID" value="Metbo_2261"/>
    </dbReference>
    <dbReference type="GeneID" id="10278727"/>
    <dbReference type="KEGG" id="mel:Metbo_2261"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_2_2"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="OrthoDB" id="49762at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="1">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="3">
        <location>
            <begin position="110"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="2">
        <location>
            <begin position="217"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="434" mass="46654" checksum="6086AFACEE3B9E79" modified="2011-05-03" version="1">MMHDVANQTLDLALKYTDQAEVYVEKEEGVDVDIKNDNVDFAKEAFTFGVGVRVIVDGRMGFSYTTNTDSLEETVKNAIFNAKSNEVDENFSFAPNSKYPKIKDIYDPKIEYLELEEIIDVAKIMLKTVVEEKCEPTSGGFSTGYSKLIMANSEGAVAKDVSSIFTGYMAVNADDGDAVSTASESDSSRFMNIDPESIALKACEIAKKSRKGQTIDTGDIPVVLDHHAAAGLLGTFYGAFNADNIQRGRSVYADKVGQQVVSSSLSIYDDGTLPGGLQSATCDGEGVPSQKTALIEDGILKNFLFDIYTANKGGVESTGNGMRSSYGDVPAVGLSNFILNFNEVNPISDLKEGVLVTDVLGAHTANPISGDFSVEAMNAFKIENGEVTYPVKKAMLSGNIFEAMGIASATESEKRSIGPFVIPRILVEKLRVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU0</accession>
    <name>F0TCU0_METLA</name>
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        <submittedName>
            <fullName evidence="2">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2266"/>
    <dbReference type="EnsemblBacteria" id="ADZ10480">
        <property type="protein sequence ID" value="ADZ10480"/>
        <property type="gene ID" value="Metbo_2266"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2266"/>
    <dbReference type="eggNOG" id="arCOG01873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264578_0_0_2"/>
    <dbReference type="OMA" id="NINHYSD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="4"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="24218" checksum="C6B4EF7F74970EE1" modified="2011-05-03" version="1">MVSAVITAAGKNRRMIEDLKSRKLEIKHKLLMDIGGKPVILHTIDNVLKSGVDECIVVLGHFNEEIGPVLEDYPDERLKIVKNTDKNVELSETLLNGVQNSSSGLVLCVAGDQPTVTAETYRNLIDTAVNYRDPENIVSVLAREATGLLQTAKGLGMPFICHLDLLLKYLPHDKNNLNPILIKMIGDGVVFYGIPSLNKLELININTYQDYIDIKDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV9</accession>
    <name>F0TCV9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645850.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2285"/>
    <dbReference type="EnsemblBacteria" id="ADZ10499">
        <property type="protein sequence ID" value="ADZ10499"/>
        <property type="gene ID" value="Metbo_2285"/>
    </dbReference>
    <dbReference type="GeneID" id="10278751"/>
    <dbReference type="KEGG" id="mel:Metbo_2285"/>
    <dbReference type="eggNOG" id="arCOG02573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_3_3_2"/>
    <dbReference type="OMA" id="TARGDMM"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="188" mass="20768" checksum="0D4FA51D8B161DE4" modified="2011-05-03" version="1">MEKVLFVCASPRKNGNTSQVISKCAEVVEKNGLETEVVYLRGKHIESCRACGKCAKIHKCKIDDGLNEIIEKIKESKGFIVATPVYFGTARGDLMAALQRIGYVSMNNGNFLSWKVGGPIAVARRGGQTSTLQELLMFYLINDMIVPGSTYWNMVFGREKGEALEDAEGIRTIERFGENVATLINKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V8</accession>
    <name>F0T5V8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644702.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09351">
        <property type="protein sequence ID" value="ADZ09351"/>
        <property type="gene ID" value="Metbo_1106"/>
    </dbReference>
    <dbReference type="GeneID" id="10277556"/>
    <dbReference type="KEGG" id="mel:Metbo_1106"/>
    <dbReference type="HOGENOM" id="CLU_2447694_0_0_2"/>
    <dbReference type="OrthoDB" id="138611at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10427" checksum="10A4AEEB3246794E" modified="2011-05-03" version="1">MPRMSSVYLNIDGLEITEVTRHYYPIGKPHFKFNRNSSNDRIYTHLKSKEGKRMKLIYNDENGIKKADSLFILSSITCKDDVITVNMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X2</accession>
    <name>F0T5X2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644716.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09365">
        <property type="protein sequence ID" value="ADZ09365"/>
        <property type="gene ID" value="Metbo_1120"/>
    </dbReference>
    <dbReference type="GeneID" id="10277570"/>
    <dbReference type="KEGG" id="mel:Metbo_1120"/>
    <dbReference type="eggNOG" id="arCOG01744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329469_0_0_2"/>
    <dbReference type="OMA" id="NANRRVK"/>
    <dbReference type="OrthoDB" id="127104at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="23743" checksum="2C939FA530261F29" modified="2011-05-03" version="1">MRSYTHIAGALVLFLVFAYIFDLNQIFVGLIFAALSSVFPDILDNLISEHRGYGHSIMWIILAVFILFFNFTMGFAIIIGLLSHILLDSITTHGVPILYPIRKTNFIVLNANRRVKTGTNQDNAVFLALVFLLITILCLNIAFTQISAINPFQSFAAQSPSTNRNMAINASNDLKTNFDFDLHLNQNDKNITVKKISENETNYLIKNVEPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z0</accession>
    <name>F0T5Z0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644734.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1139"/>
    <dbReference type="EnsemblBacteria" id="ADZ09383">
        <property type="protein sequence ID" value="ADZ09383"/>
        <property type="gene ID" value="Metbo_1139"/>
    </dbReference>
    <dbReference type="GeneID" id="59558429"/>
    <dbReference type="KEGG" id="mel:Metbo_1139"/>
    <dbReference type="eggNOG" id="arCOG10883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286357_0_0_2"/>
    <dbReference type="OrthoDB" id="120333at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="23812" checksum="B39B28344E0B5DCB" modified="2011-05-03" version="1">MVRKDDINSSKGYLLCDKCGGYYELQPGESPDDFEECECGGNLRYSKINPVKNESKGSITQKFPQNDLKSLISQNKKKSLIIICVLAIIITIPLILYVNQQTNIKYTLLGSYDAENIDYTLVHIPQGTKSIKIEYNISWNATSAGTNAFELHAYNINSGDRVPTGTLNLTNNKGFSIYEGQNKSGTYYFNNPQIKTLVLNGNGIKGTINIYTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z3</accession>
    <name>F0T5Z3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integrase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1142"/>
    <dbReference type="EnsemblBacteria" id="ADZ09386">
        <property type="protein sequence ID" value="ADZ09386"/>
        <property type="gene ID" value="Metbo_1142"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1142"/>
    <dbReference type="eggNOG" id="arCOG01245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041884_1_0_2"/>
    <dbReference type="OMA" id="HETYIKT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="133"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="373"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="423" mass="49967" checksum="2395CBE73D6EFE71" modified="2011-05-03" version="1">MLTIYELLDIVCNMKYEHIANDPDFIDFCNRRKHKTSTTNQYLHALGMYTDLIGKSLPELIKEAEDEEDEGIRLRKRTIRKYLSKFQQHIESLGYSESSKKVFTTCVRAYYNEYEIQLPKRFKNNARSDRKEKLYEDLPTLEDIRHLLKYANLTFTAIILLGLSSGMSRAEICSLKFKDFYNAIDLKPFPKDLDEFLERVKDQTNIIPFWQITRIKTENSFFTFSTPESTEAIINYIADLNRRNKKHIAQNRTTQIEITPETSLFLSSYMNRMSETVVSIAYKRMNENAGFEKKDNKSFIRPHILREVFASTLEKNKMTHLMTRWIMGHKLDSTTSAYFKADPEAVKEEYIQFLSHLTVNQNVEVKTVTTEGYDQLLKDSKEKKDKIKFMEEEIQKMKKHNEERDKLLDEIIHDKKVMGNILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>F0T608</accession>
    <name>F0T608_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Lon protease</fullName>
            <ecNumber evidence="6 7">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lon</name>
        <name evidence="14" type="ordered locus">Metbo_2301</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6 7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="6">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7 10 11">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645866.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2301"/>
    <dbReference type="EnsemblBacteria" id="ADZ10515">
        <property type="protein sequence ID" value="ADZ10515"/>
        <property type="gene ID" value="Metbo_2301"/>
    </dbReference>
    <dbReference type="GeneID" id="10278767"/>
    <dbReference type="KEGG" id="mel:Metbo_2301"/>
    <dbReference type="eggNOG" id="arCOG02161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_3_2"/>
    <dbReference type="OMA" id="KKMNPVM"/>
    <dbReference type="OrthoDB" id="7440at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01973">
        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027543">
        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="13">
        <location>
            <begin position="10"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="12">
        <location>
            <begin position="591"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 8 10">
        <location>
            <position position="677"/>
        </location>
    </feature>
    <feature type="active site" evidence="6 8 10">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <begin position="354"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="793" mass="88360" checksum="25479CB6082E4FF6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T651</accession>
    <name>F0T651_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-bisphosphatase</fullName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2345"/>
    <dbReference type="EnsemblBacteria" id="ADZ10558">
        <property type="protein sequence ID" value="ADZ10558"/>
        <property type="gene ID" value="Metbo_2345"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2345"/>
    <dbReference type="eggNOG" id="arCOG01349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_5_0_2"/>
    <dbReference type="OMA" id="RWGDERY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="30319" checksum="FE74080C05981610" modified="2011-05-03" version="1">MKPKDINFWSEVSEEIMFEAHEAVKPLIGKEEAGKTVKMGADGTPTKLIDIVAEDKVVEVLEKTGIPVTLISEEVGELKIGEGTSEAVLVVDPLDGTRNALKNIPAYGISIAISDGNLENVNDLKLSDIELGFVKNFANNDVYHAVKGDGAWLNKEKISPSKRMEVAGSSIGAYIYRADMSKIDKLCATVRSMRILGSVAIELCYVANGTYDAFLDIRGNMRVMDIAAAKLIIEESNGLVTDEKCRPLVNDLNVLERTSIIASCNKNFHTQIMSILGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T661</accession>
    <name>F0T661_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="3">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">guaAA</name>
        <name evidence="5" type="ordered locus">Metbo_2355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645919.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2355"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="EnsemblBacteria" id="ADZ10568">
        <property type="protein sequence ID" value="ADZ10568"/>
        <property type="gene ID" value="Metbo_2355"/>
    </dbReference>
    <dbReference type="GeneID" id="10278821"/>
    <dbReference type="KEGG" id="mel:Metbo_2355"/>
    <dbReference type="eggNOG" id="arCOG00087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OMA" id="GPDMDRI"/>
    <dbReference type="OrthoDB" id="79931at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 3" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="21056" checksum="2FC163B178C94086" modified="2011-05-03" version="1">MKIAVINNHGQYNHRIYRSLHYLKIPAELVPNTTPVEELREKNPLGLILGGGPSIERAGYSLDYVKEFDCPILGICLGHQIIAKAYNGEIGAAGIESYAKIKINILDEDDILKGLGTGLDVWASHKDEVCKAPENFDVIATSSICGVEAMKHPTKPVYGIQFHPEVHHTENGGVIFENFYEVCKKYHGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T683</accession>
    <name>F0T683_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644749.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1154"/>
    <dbReference type="EnsemblBacteria" id="ADZ09398">
        <property type="protein sequence ID" value="ADZ09398"/>
        <property type="gene ID" value="Metbo_1154"/>
    </dbReference>
    <dbReference type="GeneID" id="10277604"/>
    <dbReference type="KEGG" id="mel:Metbo_1154"/>
    <dbReference type="eggNOG" id="arCOG06495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782153_0_0_2"/>
    <dbReference type="OMA" id="PYIWINT"/>
    <dbReference type="OrthoDB" id="66199at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="370" mass="40420" checksum="9CA8977659C66140" modified="2011-05-03" version="1">MDDKGYAFTPLVLLLFIPVIIIGVSFSGIVNELNSLSSIAIAGDVTTTVATNVIKAIRDDTADAGRNAAYTATREVIDNYNGNNNPFFGKTAPSDSQTYIETNTLAMLNQNITNTCRALEQQTGRNITINGFAINPSDTSSVAIFDSGDMNITQSDPFGFNITLTSVPVTITQSSTSNNQSFNLNTPPMNVYVSVAKLEDPYIWINTYARNSSVIIPYPYYTQSSNLIGGNASNIYHFADKVSSGKLNYLNEELIGSNETSGFGYMPYYFPDTHGLSFFDRLENRTNNTSVSPPSARMSTFILWNPVWENVPGYTPSYLDHEYFAGINGTPINTTHSGVNTLVSDPLGSTFFLTATYKNLLGLDGTPYAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T685</accession>
    <name>F0T685_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644751.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1156"/>
    <dbReference type="EnsemblBacteria" id="ADZ09400">
        <property type="protein sequence ID" value="ADZ09400"/>
        <property type="gene ID" value="Metbo_1156"/>
    </dbReference>
    <dbReference type="GeneID" id="10277606"/>
    <dbReference type="KEGG" id="mel:Metbo_1156"/>
    <dbReference type="eggNOG" id="arCOG10451">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811439_0_0_2"/>
    <dbReference type="OMA" id="LRWEYKA"/>
    <dbReference type="OrthoDB" id="122989at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="13993" checksum="1AC828209018F4EB" modified="2011-05-03" version="1">MDLKEGWEKKALIGLGAVVVIIILYSYFAPYTGTPDNITQPQVTSTGSVTPVTFVSPSNNNSTNSSLNGNFTITAAQAQQIATNANPGYTISSTNQANIAVNGTQYSAWILSLSNGTASKTVYVNAANGNILTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T690</accession>
    <name>F0T690_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1161"/>
    <dbReference type="EnsemblBacteria" id="ADZ09405">
        <property type="protein sequence ID" value="ADZ09405"/>
        <property type="gene ID" value="Metbo_1161"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1161"/>
    <dbReference type="eggNOG" id="arCOG02644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_1_2"/>
    <dbReference type="OMA" id="HARTLKW"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="25101" checksum="F7AAC352BAF34693" modified="2011-05-03" version="1">MIVSLAKWKEREKVERQNDIINATRKLIGHKDFDEISMDEIAKEIGLGKSTLYLYFKNKESLYFAVVLKGIKIWEEMVKNGVNHSDNGLEMLISYKNANKEFSNQYPEYFSLLYSPTSIKKQFNMEKMENSQEFQEVRKVFKEILTVGIDAIQKASEEGTIRKEVDPVEAIILLSIIYNGRVNMGDWSKELLNSRGIDDEKFNSDIGDFFLKMLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D6</accession>
    <name>F0T6D6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644802.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1208"/>
    <dbReference type="EnsemblBacteria" id="ADZ09451">
        <property type="protein sequence ID" value="ADZ09451"/>
        <property type="gene ID" value="Metbo_1208"/>
    </dbReference>
    <dbReference type="GeneID" id="10277658"/>
    <dbReference type="KEGG" id="mel:Metbo_1208"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09483">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_1_2"/>
    <dbReference type="OMA" id="LGSWEVY"/>
    <dbReference type="OrthoDB" id="86909at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="5"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="338" mass="37253" checksum="75800AE7E56668E7" modified="2011-05-03" version="1">MQSRYSRQVILENIGKEGQEKLLKSSVAIVGCGALGTVAANNLARAGVGKITIIDRDFVELNNLQRQMLFDEKDVGAPKAVAAAQKVHDINSEIEVVPVIKDLNYTNAEELLENVDLVVDGTDNILTRMLVNDICVKNKIPWIYTGAIGTSGMSMNILPNKACIRCLYPGVPKAGSLPTCDTMGVLNTATVIMGSIETTEALKILLGYYDDDESTDSNLIVYDTWNHSFDTISVRKNEKCECCGNQNYEYIDSDEQEIITSLCGRNSIQITPADPKELSLKKIAENLEKLGKVKCSDFIMIFSTEETEISLFRDGRAIIKGTNDEKVARSIYARYIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E8</accession>
    <name>F0T6E8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0280 protein Metbo_2369</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0280 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2369"/>
    <dbReference type="EnsemblBacteria" id="ADZ10582">
        <property type="protein sequence ID" value="ADZ10582"/>
        <property type="gene ID" value="Metbo_2369"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2369"/>
    <dbReference type="eggNOG" id="arCOG04376">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074757_0_0_2"/>
    <dbReference type="OMA" id="YGVCTSS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01079">
        <property type="entry name" value="UPF0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037456">
        <property type="entry name" value="MA1715-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007183">
        <property type="entry name" value="UPF0280"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006421">
        <property type="entry name" value="UCP006421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="27977" checksum="A52877A7D18243E8" modified="2011-05-03" version="1">MKAKTIFEERITIGETELKVSSDLMVPELSNYIISLRSQLKGYIMKNPEFLTSFEPLTVKETLDNKLSPDHGHTSEVPLIVNLMSRAGRRADVGPMAAVAGTISQLLMGFMVEKGANFIIIDNGGDISLEINKDVVVGLYAGESSLSGELGFKIKPKQTPMGICTSSGTVGHSISFGRADSVTVFANEASIADALATSIANEAKGTKDEDAVQRSLEHAEEFKKAMRGVMVVVGESAGTLGRIPQLVQTDKKVVLGDLFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I1</accession>
    <name>F0T6I1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">Metbo_2402</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645966.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2402"/>
    <dbReference type="EnsemblBacteria" id="ADZ10615">
        <property type="protein sequence ID" value="ADZ10615"/>
        <property type="gene ID" value="Metbo_2402"/>
    </dbReference>
    <dbReference type="GeneID" id="10278874"/>
    <dbReference type="KEGG" id="mel:Metbo_2402"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="OMA" id="HRCLQMT"/>
    <dbReference type="OrthoDB" id="36053at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="PTHR13930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="51"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="35083" checksum="5315EE7309C6E32E" modified="2011-05-03" version="1">MKTSLNEGEIKALEKKGYRFAGKYKHAAAKVCHWTKKSLVDEGVCYKEKFYGIQSHRCLQMSPSIPFCHQKCSFCWRDTSITKTEWNEEFDDAKTVIDDCVEAQRNLLCGFFGNARVNKKKIKEAQDPKNAAISLAGEPMLYPDINELISEFNRRNFTTFLVTNGMTPEKLENLDDEPTQLYISLDAPNKTLYNEVCQPQINGGWELLNKSLELMKSFNSRTVIRTTCVKDLNMQLPEEYAKIITKTDPDFIEIKAYMFVGSSRDRLTFDNMPSNKEVLEFAESIAALCGRKVKDHAHESRVVLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L0</accession>
    <name>F0T6L0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease HII</fullName>
            <shortName evidence="10">RNase HII</shortName>
            <ecNumber evidence="10">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rnhB</name>
        <name evidence="13" type="ordered locus">Metbo_2431</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10 11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 10 11">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645995.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2431"/>
    <dbReference type="EnsemblBacteria" id="ADZ10644">
        <property type="protein sequence ID" value="ADZ10644"/>
        <property type="gene ID" value="Metbo_2431"/>
    </dbReference>
    <dbReference type="GeneID" id="10278903"/>
    <dbReference type="KEGG" id="mel:Metbo_2431"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OMA" id="REECRFF"/>
    <dbReference type="OrthoDB" id="105421at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00729">
        <property type="entry name" value="TIGR00729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_HII" evidence="12">
        <location>
            <begin position="4"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="23109" checksum="D925C389D315D862" modified="2011-05-03" version="1">MKIVGIDEAGRGSVLGPLVVCGVSIEEERLKYLERLKLKDSKKVTPKRRIVLYRKIKKIADCHPVHISAADIDLLRSKDVNLNEIEKIAIKQIIGNCDPDISYIDCIDVKPERFTNEIENFQENLKVVAEHGADDTYPIVSAASIVAKVERDQEISRLKKEFGEIGSGYPSDPKTVAYLKATPYKELPIFVRRSWSTVEKMRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X5</accession>
    <name>F0T6X5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1253</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644846.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1253"/>
    <dbReference type="EnsemblBacteria" id="ADZ09495">
        <property type="protein sequence ID" value="ADZ09495"/>
        <property type="gene ID" value="Metbo_1253"/>
    </dbReference>
    <dbReference type="GeneID" id="10277704"/>
    <dbReference type="KEGG" id="mel:Metbo_1253"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="VLATHEM"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="24320" checksum="882B572F1FDDDAB0" modified="2011-05-03" version="1">MNNNVLEFENVSKTYKMGDETIKALDGLNVALKRGSFKAVMGPSGSGKSTFLHVAGILDMPTSGLIQINGKETKKLSNKEQAKLRRNEIGFIFQRFNLMSQLTVLENVMLPMIEENTNKAIELLNKMGLSSKADKYPSHLSGGEQQRVAIARALINDPSIILADEPTGELDTKNANAIMQILKDLNQKDGVSIVVVTHNQASADYADEILHMSDGNFVENAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T710</accession>
    <name>F0T710_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644881.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1288"/>
    <dbReference type="EnsemblBacteria" id="ADZ09530">
        <property type="protein sequence ID" value="ADZ09530"/>
        <property type="gene ID" value="Metbo_1288"/>
    </dbReference>
    <dbReference type="GeneID" id="10277739"/>
    <dbReference type="KEGG" id="mel:Metbo_1288"/>
    <dbReference type="eggNOG" id="arCOG03190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174513_1_0_2"/>
    <dbReference type="OMA" id="CINFCIA"/>
    <dbReference type="OrthoDB" id="126855at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019214">
        <property type="entry name" value="DUF2109_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09882">
        <property type="entry name" value="DUF2109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="83" mass="9088" checksum="F0874A1243B0C1B6" modified="2011-05-03" version="1">MLIEILGIIVLLMALRTLIAEDRAARLLYLNVIGFALSGMIALYIQTPFGAIIAITFFICSTLSANAIAYSIGRNKREIVLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T737</accession>
    <name>F0T737_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646022.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10671">
        <property type="protein sequence ID" value="ADZ10671"/>
        <property type="gene ID" value="Metbo_2458"/>
    </dbReference>
    <dbReference type="GeneID" id="10278930"/>
    <dbReference type="KEGG" id="mel:Metbo_2458"/>
    <dbReference type="eggNOG" id="arCOG09717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191059_0_0_2"/>
    <dbReference type="OMA" id="FRAPKEN"/>
    <dbReference type="OrthoDB" id="128922at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="12204" checksum="E0DDF1A6DB67D167" modified="2011-05-03" version="1">MDETNVVFTVNMKHNKYKDEKTLEISGENVTIQSRQGGSVVMNFEGTMSEIMNINNFFSEVNDVEPIKVNIAGSGDVDHYFRGISDMVEDTEGLYKLRVTLQFLSTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T748</accession>
    <name>F0T748_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646033.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2469"/>
    <dbReference type="EnsemblBacteria" id="ADZ10682">
        <property type="protein sequence ID" value="ADZ10682"/>
        <property type="gene ID" value="Metbo_2469"/>
    </dbReference>
    <dbReference type="GeneID" id="10278941"/>
    <dbReference type="KEGG" id="mel:Metbo_2469"/>
    <dbReference type="eggNOG" id="arCOG04893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982163_0_0_2"/>
    <dbReference type="OMA" id="QGLCGRI"/>
    <dbReference type="OrthoDB" id="45819at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009181">
        <property type="entry name" value="UCP004929_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09872">
        <property type="entry name" value="DUF2099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004929">
        <property type="entry name" value="UCP004929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03275">
        <property type="entry name" value="methan_mark_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30521" checksum="94DF3BD3A84E519B" modified="2011-05-03" version="1">MDEHIIEALGKAKIKVKNGKVVEVGEPKIDYCPIFDKYRGIKKLTPETIKENIEFRIEDFGMCTEDRTMRMHDFLSFGVSETLTTILKEGEIDAVVSVCEGCGTVILTEPELVQGTGGRVSGLVSTCPIEKLMEEVGQDNVLDPANASIDQIAGTMKAIEMGFKKIAVTIVSADDAIRLREIEAENKDVNIFIFVAHVTEMSEENAITIFNHADVVTGCASKYIRNIGKEKEYFVAGDSIPIFGVTDTGKRFMELRIEKIGGMKDKKDPKIPKPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T794</accession>
    <name>F0T794_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Formate dehydrogenase</fullName>
            <ecNumber evidence="6">1.17.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Metbo_2515</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">AL-21</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2515"/>
    <dbReference type="EnsemblBacteria" id="ADZ10728">
        <property type="protein sequence ID" value="ADZ10728"/>
        <property type="gene ID" value="Metbo_2515"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2515"/>
    <dbReference type="eggNOG" id="arCOG01492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_2_2_2"/>
    <dbReference type="OMA" id="NLAWCHP"/>
    <dbReference type="OrthoDB" id="1029at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02790">
        <property type="entry name" value="MopB_CT_Formate-Dh_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041925">
        <property type="entry name" value="CT_Formate-Dh_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="15">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="18">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="17">
        <location>
            <begin position="226"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ADZ10728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="901" mass="99376" checksum="BBEB44456B78D41E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A1</accession>
    <name>F0T7A1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisB</name>
        <name evidence="6" type="ordered locus">Metbo_0077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0077"/>
    <dbReference type="EnsemblBacteria" id="ADZ08330">
        <property type="protein sequence ID" value="ADZ08330"/>
        <property type="gene ID" value="Metbo_0077"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0077"/>
    <dbReference type="eggNOG" id="arCOG04398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_2"/>
    <dbReference type="OMA" id="ARHGLFD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21194" checksum="8A64C19C1A30E209" modified="2011-05-03" version="1">MRKKTLNRKTSETDISVTMNLDGNGDYDINTGIEFFDHMLNSFARHGFIDLTIHANGDIGVDDHHTIEDVGILLGETYNEVIGTKRGIKRFSHALIPMDDALATVAVDISGRSYAVLDFEFKKPKVGDMSTENVEHFFESFANSAKININAKIEGENDHHKIEALFKAFARALKDASLIEHDSLPSTKGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A6</accession>
    <name>F0T7A6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiA</name>
        <name evidence="3" type="synonym">cfbB</name>
        <name evidence="6" type="ordered locus">Metbo_0082</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP + 2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP + cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="3"/>
    <dbReference type="EC" id="6.3.5.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643686.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0082"/>
    <dbReference type="EnsemblBacteria" id="ADZ08335">
        <property type="protein sequence ID" value="ADZ08335"/>
        <property type="gene ID" value="Metbo_0082"/>
    </dbReference>
    <dbReference type="GeneID" id="10276505"/>
    <dbReference type="KEGG" id="mel:Metbo_0082"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_2_1_2"/>
    <dbReference type="OMA" id="MYLTNSI"/>
    <dbReference type="OrthoDB" id="14023at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="248"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="3">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="451" mass="49464" checksum="159B4D63AA7F5F71" modified="2011-05-03" version="1">MRAVIAGTGSAVGKTTISTGIMSALSSEYNVQPFKAGPDYIDPTYHGLATGNVSRNLDSFFMSDGQIREAFERGINSSKSDMGIIEGVRGLYEGISPLGEVGSTASVAKALNAPVILILNSRSLVKSAAAVVIGFKTLDPSIRIDGVILNYVKNKKHYLKTKEAVEKLSDTPVIGGIPRKEAITVEQRHLGLVPAVERQNILQSIEKWGEEMTNNIDLDALIAIMKNSGDLPRGREPIWEEGNKKPVKIGVAMDEVFTFYYKENIESLEANKAKIIPFSPIHDETIPDVDGLYIGGGYPEIFAKELSRNSSMRKSILKFHNDAHPIYAECGGLMYLSNSINGHSMCNVFKYPSKMTKNVQALSYVISEAQNDNIILNKGEQFKGHEFHYSKIEVDGNNPKYAFKILRGRGIDGSNDGLMENNTVASYVHTHVAACPQFGLNFARNSHNKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B1</accession>
    <name>F0T7B1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="16" type="ordered locus">Metbo_0087</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643691.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0087"/>
    <dbReference type="EnsemblBacteria" id="ADZ08340">
        <property type="protein sequence ID" value="ADZ08340"/>
        <property type="gene ID" value="Metbo_0087"/>
    </dbReference>
    <dbReference type="GeneID" id="10276510"/>
    <dbReference type="KEGG" id="mel:Metbo_0087"/>
    <dbReference type="eggNOG" id="arCOG04045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="OrthoDB" id="7176at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ08340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="551" mass="58599" checksum="30AFABC0F8E9A18B" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C3</accession>
    <name>F0T7C3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="4 12">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">uppP</name>
        <name evidence="13" type="ordered locus">Metbo_0099</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="4 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643703.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0099"/>
    <dbReference type="EnsemblBacteria" id="ADZ08352">
        <property type="protein sequence ID" value="ADZ08352"/>
        <property type="gene ID" value="Metbo_0099"/>
    </dbReference>
    <dbReference type="GeneID" id="10276524"/>
    <dbReference type="KEGG" id="mel:Metbo_0099"/>
    <dbReference type="eggNOG" id="arCOG04761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_2_2"/>
    <dbReference type="OMA" id="IEGPFRD"/>
    <dbReference type="OrthoDB" id="104282at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="29875" checksum="18F54B43BC004BB8" modified="2011-05-03" version="1">MDIVQAIILGIIQGLTEFLPVSSSAHLVFMTDVLHLPQNVAFDTVLHLGTLVAVVGYFWKDLVQIIKSFISSLLDLFRGNFKQGLNDEPFKKLSWLIIIGTIPAGLAGILFKNEFEALFSNILYVGIFLIITGFLLWASERVKTGHKDVKDMTLKNAIVIGVFQALSIAPGISRSGSTIAAGLYSGLNRELAARYSFLLSIPAILGAALVQVKDITSFDATIVVFAAGFLAALIFGYLAIKLLMKIIKERTLMIFAYYCWVVGIIVIILTVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E7</accession>
    <name>F0T7E7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GAF domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643727.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0123"/>
    <dbReference type="EnsemblBacteria" id="ADZ08376">
        <property type="protein sequence ID" value="ADZ08376"/>
        <property type="gene ID" value="Metbo_0123"/>
    </dbReference>
    <dbReference type="GeneID" id="10276548"/>
    <dbReference type="KEGG" id="mel:Metbo_0123"/>
    <dbReference type="eggNOG" id="arCOG02357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582934_0_0_2"/>
    <dbReference type="OMA" id="MGEARFK"/>
    <dbReference type="OrthoDB" id="94173at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAF" evidence="1">
        <location>
            <begin position="42"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="21691" checksum="975FF8AA72945ECC" modified="2011-05-03" version="1">MKTNEQPELEKSEYGQGSGKKLIREDILDLRTSINSMLESSSLKAISDLIMDEAKKLTSSDNCYIAYVDPVNKDSVGISFSHMTGECQMYSELGEARFKIRKDGTYGGLLGYSLDTGESFFIHNPRLHPAAHGLPKGHVPVNEFLSVPVKYNQNIIGQIVLGNPKNSYTSLDVDLTDALAEIYALAIYNFLYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G4</accession>
    <name>F0T7G4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="6">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cofH</name>
        <name evidence="8" type="ordered locus">Metbo_0140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643744.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0140"/>
    <dbReference type="EnsemblBacteria" id="ADZ08393">
        <property type="protein sequence ID" value="ADZ08393"/>
        <property type="gene ID" value="Metbo_0140"/>
    </dbReference>
    <dbReference type="GeneID" id="10276566"/>
    <dbReference type="KEGG" id="mel:Metbo_0140"/>
    <dbReference type="eggNOG" id="arCOG00656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_2"/>
    <dbReference type="OMA" id="HVTNICK"/>
    <dbReference type="OrthoDB" id="26917at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="45"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="347" mass="38687" checksum="5B256C07C9F36AB5" modified="2011-05-03" version="1">MENIYERSLAGEITKTDAINLINSNPHELFDVADRLRSEIVGDEITFVANKAIDITDHCMIECAFCSFRDHIGYEMTTEEILESIREGQEMGSTEICLFGGIMPHMTVDYYSDLIKAIKSEFDIYLHALSPVEIFQTAKLSGISTKETLKTLKAAGMDTMTGASAEILVDSVREKICPNKVNTSQWLDIIREAHELGIPTTSTIMYGSIETWEDRIDHMMLLRDLQRETGGFTEFVPLTFLNENNQLGRVSQGASGMDDLKLHALARIIFGRDIPNIQVSWVKLGVRTSQIALCCGANDLGGTMIEDKISIAAGASYGEYLPKEQMIEIIEALGRVPRERTTIYGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I1</accession>
    <name>F0T7I1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644900.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1307"/>
    <dbReference type="EnsemblBacteria" id="ADZ09549">
        <property type="protein sequence ID" value="ADZ09549"/>
        <property type="gene ID" value="Metbo_1307"/>
    </dbReference>
    <dbReference type="GeneID" id="10277758"/>
    <dbReference type="KEGG" id="mel:Metbo_1307"/>
    <dbReference type="eggNOG" id="arCOG06462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091578_0_0_2"/>
    <dbReference type="OrthoDB" id="93501at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="28486" checksum="C49BDB78EBD6414F" modified="2011-05-03" version="1">MISIVCTCNNKTVLDNYLLKGLKNQREPYELIVVKDSKTDFKSAAQALNYGGEKARGEYIIFAHQDVLMENPDWLADLNKLLPELKNLGAAGIAGKSEKNPEVITNVKHGDQPHRAGEEIDKPVKVQTLDECLMVVPNKVFQKRKFDESTCEGWHLYGVDYCLMIDEMGLDVYVLPLPIIHKSSGDPFADEYYRTLGKLFKKYKKNYGTIHTTVSNWNTSYPVTLEKRRFWLTNKIYSGAKNRIKSLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q1</accession>
    <name>F0T7Q1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646084.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2520"/>
    <dbReference type="EnsemblBacteria" id="ADZ10733">
        <property type="protein sequence ID" value="ADZ10733"/>
        <property type="gene ID" value="Metbo_2520"/>
    </dbReference>
    <dbReference type="GeneID" id="10278994"/>
    <dbReference type="KEGG" id="mel:Metbo_2520"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160008_0_0_2"/>
    <dbReference type="OMA" id="WLIKVAL"/>
    <dbReference type="OrthoDB" id="108787at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="11773" checksum="A85C10A82DCF266F" modified="2011-05-03" version="1">MSDKSEKGLPKHYVSIRERFEEYGSAISDLGRTVRENGPIDEKNSQLIQLAASAAIRSEGAVHSHGKQALQNGASPDEVYQTLLLLTSTIGFPNVAAAISWIDDLFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S7</accession>
    <name>F0T7S7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="8">SHMT</shortName>
            <shortName evidence="8">Serine methylase</shortName>
            <ecNumber evidence="8">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyA</name>
        <name evidence="11" type="ordered locus">Metbo_0162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible interconversion of serine and glycine with tetrahydromethanopterin (H4MPT) serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methylenetetrahydromethanopterin + glycine + H2O = 5,6,7,8-tetrahydromethanopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:47104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643765.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0162"/>
    <dbReference type="EnsemblBacteria" id="ADZ08414">
        <property type="protein sequence ID" value="ADZ08414"/>
        <property type="gene ID" value="Metbo_0162"/>
    </dbReference>
    <dbReference type="GeneID" id="10276588"/>
    <dbReference type="KEGG" id="mel:Metbo_0162"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="OrthoDB" id="22518at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SHMT" evidence="10">
        <location>
            <begin position="9"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8 9">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="421" mass="46222" checksum="3154282B1CE4EE0D" modified="2011-05-03" version="1">MNNEEYALKIKEITKEHHNWMENSINLIASENITSTSVREALASDLSHRYAEGLSGCRLYEGCKYVDEIEDITVDLSKKIFKAEHANVQPISGVTANMASFFALAKHGDPMMALEVPVGGHISHASVSAAGIRGLKVSPHPFDEAKMNIDADAMKKDILEKKPKIVLLGGSLFLFPHPVEEAREAADEVGAKVMYDGAHVLGLIAGGCFQDPLKEGADLLVGSTHKTFPGPQGGIILCKEDIAHKIDDAVFPGVVSNHHLHHLAALGIATAEMLEFGSAYSKQIIKNAKALAQELYELGFNVLCEDQGFTESHQLAMDVSNIGRAAKLAKDLEANNVILNKNLFPWDDVNRSDDPSGIRIGTQEITRRGLKEAEMAEIAQFIKKVTVDGKNVEEEVTEFIKQYNTVKYAFKEDEAYNYVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T6</accession>
    <name>F0T7T6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.85</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.85" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0171"/>
    <dbReference type="EnsemblBacteria" id="ADZ08423">
        <property type="protein sequence ID" value="ADZ08423"/>
        <property type="gene ID" value="Metbo_0171"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0171"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="OrthoDB" id="33452at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="1">
        <location>
            <begin position="8"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="334" mass="36513" checksum="83BA9BA4069E8E69" modified="2011-05-03" version="1">MKVWDMYKIAVIPGDGIGKEVMEATLHVLESSNVDFEYSFADAGDEYAEKTGIPLSEETVNLVKQSQACLFGAAGETAADVIVKLRKELDLYVNLRPVKSYPGTKYLFEGLDFVIVRENTEGLYSGLEEYTQDGATATRVITKAASERICKFAFEYAEKTARSKVTAVHKANVLKKTDGVFKDSFYKVAENFSHIETDDRYVDATAMFFITQPQMFDVVVTTNLYGDILSDEGAGLVGGLGLIPSANIGETNGLFEPVHGSAPDIAGKGLANPSAMILSAVLMLEYLDEYDEARRLENALLKVLDEGKVVTKDLGGESSTMEMAEEVRSKLEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U4</accession>
    <name>F0T7U4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0179</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643782.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0179"/>
    <dbReference type="EnsemblBacteria" id="ADZ08431">
        <property type="protein sequence ID" value="ADZ08431"/>
        <property type="gene ID" value="Metbo_0179"/>
    </dbReference>
    <dbReference type="GeneID" id="10276605"/>
    <dbReference type="KEGG" id="mel:Metbo_0179"/>
    <dbReference type="eggNOG" id="arCOG00946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="OMA" id="YHFYPGS"/>
    <dbReference type="OrthoDB" id="39920at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="334" mass="37653" checksum="85F1F4F867A2FACB" modified="2011-05-03" version="1">MKKEAILYDKIGKGLNCKVCERRCLISKGKKGFCEMRENIGGKLFSLNYGAASSVAVDPIEKKPLFNFYPGSTVLSLGSVGCNFRCKHCQNWSISQASLEDMSLREILPEDAVKLAKMYKCKSIAWTYNEPTMWLEYTIDSAVLAKKQNLKTVYVSNGYMTKEALDTVGPYLDAVNVDLKGMSEEFYKELCDARLQPVLDNIKRIYDKKIHLEITNLIIPGYNSSEENIRSLVSFMVDEVGVEVPLHFTRFFPYYELKNLPPTAIADLELAYKIAKDEGMKYVYVGNVSSSDGENTYCYSCGKLLIGRDSYTIQENNLKNGQCPECGADVDIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V3</accession>
    <name>F0T7V3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643791.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0188"/>
    <dbReference type="EnsemblBacteria" id="ADZ08440">
        <property type="protein sequence ID" value="ADZ08440"/>
        <property type="gene ID" value="Metbo_0188"/>
    </dbReference>
    <dbReference type="GeneID" id="10276614"/>
    <dbReference type="KEGG" id="mel:Metbo_0188"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="OMA" id="EWDDWME"/>
    <dbReference type="OrthoDB" id="72076at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="22"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="22087" checksum="74301ECFFFED6E9E" modified="2011-05-03" version="1">MIEYKGITIYTNEEVYEPAEDTFLFAENLELNRKDDVLEIGTGTGLIAICASQNSRKVIATDINEAAIKCALKNVITHRAYNVELREGNLFEPVAEEKFDLVLFNTPYLPTSEEEQLEGQINTAFDGGLDGRETIDAFLDGVKDVLKKEGRIQMVQSSLADNEKTLQKLRDLGFQAEITAKEKCFFEEIVVITGKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V9</accession>
    <name>F0T7V9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">50S ribosomal protein L21e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl21e</name>
        <name evidence="4" type="ordered locus">Metbo_0194</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643797.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0194"/>
    <dbReference type="EnsemblBacteria" id="ADZ08446">
        <property type="protein sequence ID" value="ADZ08446"/>
        <property type="gene ID" value="Metbo_0194"/>
    </dbReference>
    <dbReference type="GeneID" id="10276620"/>
    <dbReference type="KEGG" id="mel:Metbo_0194"/>
    <dbReference type="eggNOG" id="arCOG04129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="OMA" id="KGMPHRR"/>
    <dbReference type="OrthoDB" id="107212at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_L21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_L21e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_L21e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="PTHR20981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11235" checksum="7CC52152494725D3" modified="2011-05-03" version="1">MVKRSKGSRSKTRFKLRKTIRPGRTNPITKKIQTFDESDMVHIIIDPSIHKGQPHPRFHGKTGKVGERRGNAYIVEINDGNKPKKLIIRPEHLKMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y7</accession>
    <name>F0T7Y7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Methionine aminopeptidase</fullName>
            <shortName evidence="6">MAP</shortName>
            <shortName evidence="6">MetAP</shortName>
            <ecNumber evidence="6 7">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">map</name>
        <name evidence="9" type="ordered locus">Metbo_0222</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6 7">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643825.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0222"/>
    <dbReference type="EnsemblBacteria" id="ADZ08474">
        <property type="protein sequence ID" value="ADZ08474"/>
        <property type="gene ID" value="Metbo_0222"/>
    </dbReference>
    <dbReference type="GeneID" id="10276650"/>
    <dbReference type="KEGG" id="mel:Metbo_0222"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OMA" id="FPFAERW"/>
    <dbReference type="OrthoDB" id="82853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="8">
        <location>
            <begin position="4"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="304" mass="33627" checksum="745CD0F02A555AD3" modified="2011-05-03" version="1">MIEAYEKSGKIVSKVRKMATDYIEADMKILELVDFVESNIIEMGAKPAFPCNISINEITAHYTSPPGDESTIKDGDLVKIDLGAHVDGYIADSAITVLVGDSDTVLTETENELNHKLIQTAQDALESAINTIKAGVELGEIGTAIEETITQNGLNTIPSLSGHEVDQYLLHSGISVPNKKEENHHKIEEGDVLAIEPFVTYGSGLLKDVNETNIFKFLKNRPMRMVHAKKLLHNIVSDYGSLEFCQRWLINDNNKNHINMAMRQLINSGAIYAYHVLKEKSDARVAQAEHTVIVEKDGCKVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z1</accession>
    <name>F0T7Z1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.176</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.176" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644968.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1377"/>
    <dbReference type="EnsemblBacteria" id="ADZ09617">
        <property type="protein sequence ID" value="ADZ09617"/>
        <property type="gene ID" value="Metbo_1377"/>
    </dbReference>
    <dbReference type="GeneID" id="10277828"/>
    <dbReference type="KEGG" id="mel:Metbo_1377"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="OrthoDB" id="44046at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033814">
        <property type="term" value="F:propanoyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050632">
        <property type="term" value="F:propionyl-CoA C2-trimethyltridecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
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        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="2">
        <location>
            <begin position="258"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="383" mass="40293" checksum="039036D0F1CC9CF2" modified="2011-05-03" version="1">MRDVAIIGVSQTKFGELWEVSFRDMITEAGMKAIGDAGIEGDELEAMYVGNMSAGLFVEQEHIASLIADHAGLTPIPSARVEAACASGGLALRNGIMAVASGYHDIVISAGVEKMTDVVDPTPAIATASDQEWEAQQGVTFPSLYAMMAKRHMHEYGTTREQLAMVSVLNHKHGAKNPLAQYPMEISVDSVLNSSIVADPLRLLDCSPVSDGAAAVILCPAEDARKYTDTPVYVKASTQASGTIALHDRKDITTIDSTVNASRKAYEISGYTPKDIGGVEVHDCFSINGLLAIEDLGFVEKGQGGPAIEDGMTELDGEIPVNPSGGLKARGHPLGATGIAQAAEIVWQLRGEAGKRQIEGIEVGMTHNIGGTGGTAAVHILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T877</accession>
    <name>F0T877_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_0237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643840.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0237"/>
    <dbReference type="EnsemblBacteria" id="ADZ08489">
        <property type="protein sequence ID" value="ADZ08489"/>
        <property type="gene ID" value="Metbo_0237"/>
    </dbReference>
    <dbReference type="GeneID" id="10276667"/>
    <dbReference type="KEGG" id="mel:Metbo_0237"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087235_1_0_2"/>
    <dbReference type="OMA" id="HEHIYIA"/>
    <dbReference type="OrthoDB" id="98686at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="205" mass="23472" checksum="97DBC19B0AA149BE" modified="2011-05-03" version="1">MKLKELDLTKYDINQISEMIYETDQNLFGIFLDKNPIQAVSKLKKLIEAGKNCYGHEHIYIAEDEDGTIQGVLVAFRGDEIAFVQEAKIFKATMNFKDFFKLTFIKPIYDSITASSIQGDDFYIGNLVVSANLRGKGIGSKIIEQSFQLARDKKCKRVLLDVIFENTGAKKLYERIGFKKCCEKNLWWSSKSDGTYGMEYPLKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T882</accession>
    <name>F0T882_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0242"/>
    <dbReference type="EnsemblBacteria" id="ADZ08494">
        <property type="protein sequence ID" value="ADZ08494"/>
        <property type="gene ID" value="Metbo_0242"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0242"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_4_2"/>
    <dbReference type="OMA" id="FNTHEHF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="16"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="22316" checksum="B8C9D2ABD5CBAFF8" modified="2011-05-03" version="1">MEIIDDIVMIEGIGNDSNVYIFDDIIVDTGTGNNCKYIIESISKAGLSLDDLSLIVNTHNHYDHMGGNNCFDLDVAMHIEDALAVENQDSNATVANMFGRSMEGFKVTRKLKEGDKIHNFEILHTPGHTKGGICLYDGKTLISGDTVFAGGGFGRTDLGGSVEEMKNSLKRLSQLEVQNLLPGHGPWTENGSEHIRLSYRMLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T883</accession>
    <name>F0T883_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643846.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0243"/>
    <dbReference type="EnsemblBacteria" id="ADZ08495">
        <property type="protein sequence ID" value="ADZ08495"/>
        <property type="gene ID" value="Metbo_0243"/>
    </dbReference>
    <dbReference type="GeneID" id="10276675"/>
    <dbReference type="KEGG" id="mel:Metbo_0243"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="OMA" id="QRCNVVI"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="136"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23178" checksum="59951CFB722D2731" modified="2011-05-03" version="1">MYVVIMGGGRVGLNLAASLVASGHDVTLIENDSNLCNNAAGELDALIICGNGTDIKTLEEANISDADVFVAATGHDEANLLSSILVKEYNPKKIIARVSNPDHEDAFKKVGIDDVISPELTAASYLEKLIIRPKIADLIIIGQGNAELLEIQVENKNVIGKKVVDLSPTDDYIIAALYKNGEINIPKKDDILNRGNRISVLVKSNAVKKTTKLFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T891</accession>
    <name>F0T891_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysylphosphatidylglycerol synthetase/UPF0104</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0251</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643854.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0251"/>
    <dbReference type="EnsemblBacteria" id="ADZ08503">
        <property type="protein sequence ID" value="ADZ08503"/>
        <property type="gene ID" value="Metbo_0251"/>
    </dbReference>
    <dbReference type="GeneID" id="10276683"/>
    <dbReference type="KEGG" id="mel:Metbo_0251"/>
    <dbReference type="eggNOG" id="arCOG00899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048072_1_1_2"/>
    <dbReference type="OMA" id="WFGECAA"/>
    <dbReference type="OrthoDB" id="36454at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="338" mass="37427" checksum="0591F4E0CB424C28" modified="2011-05-03" version="1">MNRKTIGLLIVGIAILAVMIIFIGPGKIEEALKLANPWYLLLAVAVQFIIYGMWTQRWSITIRSLGISIRKKHIFPMLMVGLAINNITPSGRGGGEPVRAYMLGKYTKSPMENAFATVIADRGLDTFPFVLLAIITILYSVLSLKLPEWIVIALIISLIVLVIVFFIILFMCINESVGQRITLWFVGVFKRFSKKKHYDIEDKAINAVKGFQSSMNIMIRDRRVLMYGLPLSFVIWFMEIIRVYIVFSSFGTPVPLGIIAAVFVIATLIGMIPLLPGGVGAVDGIMIILYSAAGVPPSISAAATIVERLISFWMTSFIGMGMLPYVGSGVMEEFSDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T894</accession>
    <name>F0T894_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="16">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="16">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="16">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">nnrE</name>
        <name evidence="14" type="synonym">nnrD</name>
        <name evidence="18" type="ordered locus">Metbo_0254</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AL-21</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15 16">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 16">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 16">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15 16">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="16"/>
    <dbReference type="EC" id="5.1.99.6" evidence="16"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0254"/>
    <dbReference type="EnsemblBacteria" id="ADZ08506">
        <property type="protein sequence ID" value="ADZ08506"/>
        <property type="gene ID" value="Metbo_0254"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0254"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B3</accession>
    <name>F0T8B3_METLA</name>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0273</name>
    </gene>
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            <taxon>Archaea</taxon>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Metbo_0273"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0273"/>
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    <dbReference type="OMA" id="CDPCFTC"/>
    <dbReference type="OrthoDB" id="4328at2157"/>
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        <property type="component" value="Chromosome"/>
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        <location>
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            <end position="298"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="377" mass="42429" checksum="A4C4D087AD0EF22E" modified="2011-05-03" version="1">MNENNKITEKNVIETEVTMGPVHSAAIEPYRVRLFVEDEMVKDAEITVGVNHRGIERIMEGLPVEKANSLTEKICGICSNSHIWNSVLVAEKAIGIDVPERAQYIRVIMEELERLHSHLLYLAHGNEVLGHETFAMRIFYIRETVMELLRMIGGNRVQYGASIIGGVRPRCELDAMRIQKISEGMDIIEEKILDFADRFVSDPMIMSRITGVGVLTRKQAIELAASGPTLRSTGVETDVRTEMEAYEPFDFNVITQDDGDVKSNLLMRVLEIPEAINIIRQAIKHLPDGKITNRSWDMQDAPITKSYIEVPRGKLYHSYALEDGRVRNSIVRTPSITNIGAMQYAAVGHHITDAQLAIVQCDPCFTCTDRAIQIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F4</accession>
    <name>F0T8F4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09705">
        <property type="protein sequence ID" value="ADZ09705"/>
        <property type="gene ID" value="Metbo_1469"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1469"/>
    <dbReference type="HOGENOM" id="CLU_2730410_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="8315" checksum="B9EFADFCEBDCC06D" modified="2011-05-03" version="1">MNDIWIIILILEGTIGIILSLFKPNHYYFVTFGLLLFSLGIYGAIKKYWSVFNNRSSPKYLVFNNFLNTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F8</accession>
    <name>F0T8F8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645060.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1474"/>
    <dbReference type="EnsemblBacteria" id="ADZ09709">
        <property type="protein sequence ID" value="ADZ09709"/>
        <property type="gene ID" value="Metbo_1474"/>
    </dbReference>
    <dbReference type="GeneID" id="10277925"/>
    <dbReference type="KEGG" id="mel:Metbo_1474"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OMA" id="MGPPPWA"/>
    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="34"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="19854" checksum="2423F96BD70839CA" modified="2011-05-03" version="1">MEENNDKFHDHEIYDPCHETIRGMQKLGGLRMWIMHVLDENGLTNGAEIMEAVQAHTISPNGPYKNAGPLPGSVYPMLKKMVAEDIIVKNSKGQYDLTENGRKLNSKLLGRFKEDKPEENIRKVWSIKNILAEMENDVSYLEDIKTEKLNPEKERIERLKERINEIYETLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G9</accession>
    <name>F0T8G9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1487</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645071.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1487"/>
    <dbReference type="EnsemblBacteria" id="ADZ09720">
        <property type="protein sequence ID" value="ADZ09720"/>
        <property type="gene ID" value="Metbo_1487"/>
    </dbReference>
    <dbReference type="GeneID" id="10277938"/>
    <dbReference type="KEGG" id="mel:Metbo_1487"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="FNWPGHP"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="24401" checksum="53FFE7180C26BCC9" modified="2011-05-03" version="1">MNNIITIKDLNKTYDKGRTHALKDINMDIKKGISISITGPSGSGKSSLLNMIGTLDTPDNGQVIIDGQDTSKIKHINVFRRSKVGFVFQLHNLIPTISARENIEMAMYGTGLSPETMKTRALDLLKMVDMENLAVQKPNILSGGERQRVAVARALANDPPIILADEPTGSLDTKNGELILNLLQHHQREMGATLIIVTHDPKIAQQTDESIEIVDGKIDLGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J4</accession>
    <name>F0T8J4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1514</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645096.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1514"/>
    <dbReference type="EnsemblBacteria" id="ADZ09745">
        <property type="protein sequence ID" value="ADZ09745"/>
        <property type="gene ID" value="Metbo_1514"/>
    </dbReference>
    <dbReference type="GeneID" id="24964560"/>
    <dbReference type="KEGG" id="mel:Metbo_1514"/>
    <dbReference type="eggNOG" id="arCOG02352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OMA" id="ENHHGFI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="398"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="473"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="578"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="800" mass="91996" checksum="F40C7E63271B4D77" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M6</accession>
    <name>F0T8M6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="8">PRA-PH</shortName>
            <ecNumber evidence="8">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisE</name>
        <name evidence="9" type="ordered locus">Metbo_0316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643919.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0316"/>
    <dbReference type="EnsemblBacteria" id="ADZ08568">
        <property type="protein sequence ID" value="ADZ08568"/>
        <property type="gene ID" value="Metbo_0316"/>
    </dbReference>
    <dbReference type="GeneID" id="10276753"/>
    <dbReference type="KEGG" id="mel:Metbo_0316"/>
    <dbReference type="eggNOG" id="arCOG02677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_0_0_2"/>
    <dbReference type="OMA" id="FTHEKGE"/>
    <dbReference type="OrthoDB" id="110768at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11353" checksum="B8DEDF081AB5B084" modified="2011-05-03" version="1">MKNKDSIIRELYQVLEERRDNPIDSYTSNIMKDSDKKAEDKILEKVGEESAELIIASKNDENLVYEATDLIFHALLLLVYKGVEIDDIFDEFERRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P9</accession>
    <name>F0T8P9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein synthesis factor GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0339</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643942.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0339"/>
    <dbReference type="EnsemblBacteria" id="ADZ08591">
        <property type="protein sequence ID" value="ADZ08591"/>
        <property type="gene ID" value="Metbo_0339"/>
    </dbReference>
    <dbReference type="GeneID" id="10276776"/>
    <dbReference type="KEGG" id="mel:Metbo_0339"/>
    <dbReference type="eggNOG" id="arCOG01562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012821_1_2_2"/>
    <dbReference type="OMA" id="FRFIQRP"/>
    <dbReference type="OrthoDB" id="21155at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039263">
        <property type="entry name" value="GTPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="PTHR43721:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="116"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="529" mass="59245" checksum="ACBFAFD2A585C068" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R2</accession>
    <name>F0T8R2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0352</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643955.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0352"/>
    <dbReference type="EnsemblBacteria" id="ADZ08604">
        <property type="protein sequence ID" value="ADZ08604"/>
        <property type="gene ID" value="Metbo_0352"/>
    </dbReference>
    <dbReference type="GeneID" id="10276789"/>
    <dbReference type="KEGG" id="mel:Metbo_0352"/>
    <dbReference type="eggNOG" id="arCOG04858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="OMA" id="IMGHRQP"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="28290" checksum="11155CDF88A6A977" modified="2011-05-03" version="1">MAQYYPGTTKVAQNRRNLCNPDYDLEKLREISDEDVVKILGHRAPGEEYPSVHPPLEEMDEPEDAIREMVEPVDGAKAGDRVRYIQFADSMYFAPAQPFARSRAYLCRYRGSDAGTLSGRQIIEARERDLEKISKELLETEFFDPARTGIRGKSVHGHSLRLDEDGMMFDMVRRQVLNKATGNVEMVKNQIGDELDEPVILGEPLEEAVLKEKTTIYRKDGEAYKDDTDAVEVLHRIHVTRSNGGYGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T2</accession>
    <name>F0T8T2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643975.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0372"/>
    <dbReference type="EnsemblBacteria" id="ADZ08624">
        <property type="protein sequence ID" value="ADZ08624"/>
        <property type="gene ID" value="Metbo_0372"/>
    </dbReference>
    <dbReference type="GeneID" id="10276809"/>
    <dbReference type="KEGG" id="mel:Metbo_0372"/>
    <dbReference type="eggNOG" id="arCOG04856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200895_2_0_2"/>
    <dbReference type="OMA" id="LDLEGHK"/>
    <dbReference type="OrthoDB" id="130371at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019300">
        <property type="entry name" value="CooT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10133">
        <property type="entry name" value="CooT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="6693" checksum="FC95FE8973F1C495" modified="2011-05-03" version="1">MCESTVYSTENSMIMEDVLNIKIDGNKIYLTDILGMKKELSGVIVEIDLDKHGIYIKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V3</accession>
    <name>F0T8V3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645132.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1553"/>
    <dbReference type="EnsemblBacteria" id="ADZ09781">
        <property type="protein sequence ID" value="ADZ09781"/>
        <property type="gene ID" value="Metbo_1553"/>
    </dbReference>
    <dbReference type="GeneID" id="24964564"/>
    <dbReference type="KEGG" id="mel:Metbo_1553"/>
    <dbReference type="eggNOG" id="arCOG07683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057493_0_0_2"/>
    <dbReference type="OrthoDB" id="113959at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="356" mass="41013" checksum="8E89466A45AA3AE1" modified="2011-05-03" version="1">MILVIGENNKLEKLINEVNFSELGIMERKDLQEWIFECPEILGEDLLIVTKEYDKFDKTNKRLDILAIDHDGKLVVIELKRDVANAFVDLQAIHYAAYCSTFTLDQVAEIRTEDNNKSKDVNKEEIIDFIENKEFSDFDNQPRIIIVANSYKEETLAAVLWLRDNGIDISCVKLESHKLDEKIVVTPDIIVPIPEAKEFMVYREQKSKTLSKGKITEKTFFEHLNQYGTHFFEELFKFSDEKDLILNWGGTGFSLNVRIGNEKVSLLQGYCDLKVTGQTMNSTVSMISKKVENGDIIVSDYVEKTLDLNIFRKVGNGFVFDLERELTDVEWSKFKDVLSHTIENIKKNGVKLSDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T933</accession>
    <name>F0T933_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Elongation factor 1-beta</fullName>
            <shortName evidence="7">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ef1b</name>
        <name evidence="9" type="ordered locus">Metbo_0403</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644006.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0403"/>
    <dbReference type="EnsemblBacteria" id="ADZ08655">
        <property type="protein sequence ID" value="ADZ08655"/>
        <property type="gene ID" value="Metbo_0403"/>
    </dbReference>
    <dbReference type="GeneID" id="10276840"/>
    <dbReference type="KEGG" id="mel:Metbo_0403"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_0_0_2"/>
    <dbReference type="OMA" id="VMPNSPE"/>
    <dbReference type="OrthoDB" id="124073at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="PTHR39647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="SSF54984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF1_GNE" evidence="8">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="9677" checksum="68BA02F03A7E39C4" modified="2011-05-03" version="1">MGEVVATIKLMPESPEIDLEKVKAEITSSIPENTELHSIEEEPIAFGLVAINLMVIVDDGEGGTEAVEENLSKITEIASIEVVDVRRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T955</accession>
    <name>F0T955_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulatory protein Metbo_0425</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644028.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0425"/>
    <dbReference type="EnsemblBacteria" id="ADZ08677">
        <property type="protein sequence ID" value="ADZ08677"/>
        <property type="gene ID" value="Metbo_0425"/>
    </dbReference>
    <dbReference type="GeneID" id="10276862"/>
    <dbReference type="KEGG" id="mel:Metbo_0425"/>
    <dbReference type="eggNOG" id="arCOG04152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075726_0_0_2"/>
    <dbReference type="OMA" id="PGVVYFR"/>
    <dbReference type="OrthoDB" id="76134at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="Arc_TR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="137"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="38035" checksum="4174706C56A067D7" modified="2011-05-03" version="1">MQKANISPQRDQVLIEINDLLSNHGFETSNIYDRSCFDMVARKEFELLLLKILINIDGFTGSQAEEIKKVADTFLASPLVVGIKSKNEYLEEDVIYERHGIPVIARETLRNMVVDDIYPEVFADRGGYYVQIDGEIIKQVRETQNISRKDLADKAHVSKETIYKYERGMVRAFPETAMMLESILNMKITLSVNLLSVPEVEKRADVRDTNTPKPELSVENQTTLQNLGFGVISTNRTPFDALAKKESTGKLTKKDSTVITNLEKNRNQQMLKRMALNVKDLSGITGTDAVFLLEDKKPLKCIEGVPVVYNWEIKEMEHSKEFLKLVRERRDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0T973</accession>
    <name>F0T973_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08695">
        <property type="protein sequence ID" value="ADZ08695"/>
        <property type="gene ID" value="Metbo_0443"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0443"/>
    <dbReference type="eggNOG" id="arCOG10148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_214689_0_0_2"/>
    <dbReference type="OMA" id="MITIGTI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="5837" checksum="7A29087FBA0A7400" modified="2011-05-03" version="1">MSVDPLNELFKDVEDPDKRAKLFLLVSAAMIISTILIVVGTIIFILWALHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T985</accession>
    <name>F0T985_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645187.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09836">
        <property type="protein sequence ID" value="ADZ09836"/>
        <property type="gene ID" value="Metbo_1609"/>
    </dbReference>
    <dbReference type="GeneID" id="10278066"/>
    <dbReference type="KEGG" id="mel:Metbo_1609"/>
    <dbReference type="eggNOG" id="arCOG14558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930228_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="6854" checksum="8DC1981E6ADABF36" modified="2011-05-03" version="1">MYRKINIGCPIDIYVFYCISYIKDGNGGEIILVSFELQHWCCGPKGCELEIVYGEMIDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T994</accession>
    <name>F0T994_METLA</name>
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        </submittedName>
    </protein>
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    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645196.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ09845">
        <property type="protein sequence ID" value="ADZ09845"/>
        <property type="gene ID" value="Metbo_1618"/>
    </dbReference>
    <dbReference type="GeneID" id="10278075"/>
    <dbReference type="KEGG" id="mel:Metbo_1618"/>
    <dbReference type="HOGENOM" id="CLU_161125_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="127" mass="14063" checksum="0EAC6888E3E98228" modified="2011-05-03" version="1">MKKYRLEITIVILSVIIFISIFGEVNPLIKQDLDDKIVEYNTPLVEQAINGSAVYITGVVRDDSKENFDDVLLNVYGIGSQGQRIDSKIIKIDRIESDSNVNYNVTLENSSAVVAGDVKVLNATEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C2</accession>
    <name>F0T9C2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1647</name>
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    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645224.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09873">
        <property type="protein sequence ID" value="ADZ09873"/>
        <property type="gene ID" value="Metbo_1647"/>
    </dbReference>
    <dbReference type="GeneID" id="10278104"/>
    <dbReference type="KEGG" id="mel:Metbo_1647"/>
    <dbReference type="HOGENOM" id="CLU_2695800_0_0_2"/>
    <dbReference type="OrthoDB" id="140356at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8399" checksum="4DCD2E9737BDE069" modified="2011-05-03" version="1">MEEHEIKIIKLVAKAENGISEREISEKLKIKCPLLRSYIHDLRHKRHIKYAGKGSDGHVVELTREGVALLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C6</accession>
    <name>F0T9C6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1651</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645228.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1651"/>
    <dbReference type="EnsemblBacteria" id="ADZ09877">
        <property type="protein sequence ID" value="ADZ09877"/>
        <property type="gene ID" value="Metbo_1651"/>
    </dbReference>
    <dbReference type="GeneID" id="10278108"/>
    <dbReference type="KEGG" id="mel:Metbo_1651"/>
    <dbReference type="eggNOG" id="arCOG01338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="OMA" id="SFMSQSG"/>
    <dbReference type="OrthoDB" id="2789at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="4">
        <location>
            <begin position="485"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="695" mass="74805" checksum="F88F2DBD451E86E8" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E1</accession>
    <name>F0T9E1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Integral membrane sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645243.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1666"/>
    <dbReference type="EnsemblBacteria" id="ADZ09892">
        <property type="protein sequence ID" value="ADZ09892"/>
        <property type="gene ID" value="Metbo_1666"/>
    </dbReference>
    <dbReference type="GeneID" id="25394740"/>
    <dbReference type="KEGG" id="mel:Metbo_1666"/>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027558_0_0_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
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        <property type="entry name" value="HATPase_C_sf"/>
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    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="334"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="282"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="547" mass="61047" checksum="F8D8B568A6D1C655" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E4</accession>
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        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Metbo_1669</name>
    </gene>
    <organism evidence="3 4">
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            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1669"/>
    <dbReference type="EnsemblBacteria" id="ADZ09895">
        <property type="protein sequence ID" value="ADZ09895"/>
        <property type="gene ID" value="Metbo_1669"/>
    </dbReference>
    <dbReference type="GeneID" id="10278126"/>
    <dbReference type="KEGG" id="mel:Metbo_1669"/>
    <dbReference type="eggNOG" id="arCOG03177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_0_2"/>
    <dbReference type="OMA" id="HPGIIQE"/>
    <dbReference type="OrthoDB" id="96474at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HTHMARR"/>
    </dbReference>
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        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="18543" checksum="67DBF47BFFC09306" modified="2011-05-03" version="1">MGEKQLSKEKSGKTRSKGNLSAEEISEMSIGTLISSISRAHLRYLFTEIEKHGITGGQYQFLLGLSKQDGITQEELASNFHMNQSTIARALKKLEDAEMVLRKTDDTNRRKNIITVTDKGRKVASKIQLTDEKWEENVHSLSNAEKSTFKEMLRKVLQESLGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G6</accession>
    <name>F0T9G6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-CoA decarbonylase/synthase complex subunit alpha</fullName>
            <shortName evidence="6">ACDS complex subunit alpha</shortName>
            <ecNumber evidence="6">1.2.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ACDS complex carbon monoxide dehydrogenase subunit alpha</fullName>
            <shortName evidence="6">ACDS CODH subunit alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cdhA</name>
        <name evidence="8" type="ordered locus">Metbo_0462</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 7 [4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </cofactor>
        <text evidence="6">Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644065.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0462"/>
    <dbReference type="EnsemblBacteria" id="ADZ08714">
        <property type="protein sequence ID" value="ADZ08714"/>
        <property type="gene ID" value="Metbo_0462"/>
    </dbReference>
    <dbReference type="GeneID" id="10276900"/>
    <dbReference type="KEGG" id="mel:Metbo_0462"/>
    <dbReference type="eggNOG" id="arCOG02428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_361186_0_0_2"/>
    <dbReference type="OMA" id="LCIGHNV"/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
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                <person name="Zinder S."/>
                <person name="Liu W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0502"/>
    <dbReference type="EnsemblBacteria" id="ADZ08754">
        <property type="protein sequence ID" value="ADZ08754"/>
        <property type="gene ID" value="Metbo_0502"/>
    </dbReference>
    <dbReference type="GeneID" id="10276942"/>
    <dbReference type="KEGG" id="mel:Metbo_0502"/>
    <dbReference type="eggNOG" id="arCOG03224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051457_0_0_2"/>
    <dbReference type="OMA" id="IRWFAVN"/>
    <dbReference type="OrthoDB" id="29315at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032266">
        <property type="entry name" value="HIGH_NTase1_ass"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16581">
        <property type="entry name" value="HIGH_NTase1_ass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HIGH_NTase1_ass" evidence="3">
        <location>
            <begin position="152"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="430" mass="48945" checksum="559BE0B8C7BBD6B1" modified="2011-05-03" version="1">MIGISADFDPVHLGHVKLIDKARELADKKNEDVVIYLNKGFSANHAPFFVSYDARRRMALEAGADEVIPIEGLHHRLTLAYTVPIRIAMMIEDGVVDYVDAADVSTSKIKKYASGFASKGIFSGIPRTLPNRNVIRWFAVNEFLKKKYGKNLKFHIIPEHKIKGDKISGRMIRREILENNMKIPEEVQKLLPDSTVRILEKEIKKGTIPGKRNIRDLTKRLNTFSRANLNNIAHMNADAVNSIVAGRSYQNEDKIWASFRMAGYGPVLTRLAASAVEMNVTREEVYDLIKNYEDKGIIPPDMTVDQVIQRAWYVSSMSSKGYSASEAHNKFRSGVKSTLKRVKFFDAGMHLRSFETESLKQDMDALFYVDKNDRLCCEIRAEDRKIKSPLRLPAHHVTYLRLLIDSHFIPITGKLIEKPEGWRVRISVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q9</accession>
    <name>F0T9Q9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1714"/>
    <dbReference type="EnsemblBacteria" id="ADZ09938">
        <property type="protein sequence ID" value="ADZ09938"/>
        <property type="gene ID" value="Metbo_1714"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1714"/>
    <dbReference type="eggNOG" id="arCOG00734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085608_0_0_2"/>
    <dbReference type="OMA" id="DIMHEDM"/>
    <dbReference type="OrthoDB" id="85881at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016723">
        <property type="entry name" value="Tscrpt_reg_ArsR_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018357">
        <property type="entry name" value="Trans_reg_ArsR_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="182"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="30204" checksum="F4570AD51714EEB8" modified="2011-05-03" version="1">MNMDKINNRQIDPSNSFKDINSKLDVIHREVTRIRERSNQEHLDLIILNTRKDLANSMAVYVTEDIETDLERGMVSECHLKKTCKQTFKDFLDRNVEIIKQVQDMDKKTMDETYSKLDEMKNDAPYEKCRFCIDEVSSLLQKQLKLIESLKLYQGPEFEETDLTPFSEELIVKDVLEPLSNKQRLQIIQSMATETKTYSELSEITGLRGGNLLFHIQKLQDGGLIFQRHERGDYMVTKKGYAMLNALKNMHKFLENEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X8</accession>
    <name>F0T9X8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644152.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08801">
        <property type="protein sequence ID" value="ADZ08801"/>
        <property type="gene ID" value="Metbo_0549"/>
    </dbReference>
    <dbReference type="GeneID" id="10276994"/>
    <dbReference type="KEGG" id="mel:Metbo_0549"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582902_0_0_2"/>
    <dbReference type="OMA" id="MKASEFI"/>
    <dbReference type="OrthoDB" id="106208at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="91"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="168" mass="18781" checksum="6897536E0D5073CF" modified="2011-05-03" version="1">MKASEFIGMKVIDKEANEIGKVAEIAVLFKQCLLDKVFISVGSALNKKFLVVVDDDIAEVGDYLQLKLNTQEIEEQVKVDKIEKFLGKGTRYNEFIGKTVLNKTGMDVGKISDLMIDPKGCLIHNIVVTTGGTFNKKHLMISDEDIEAMGDYLIIKLDQDQINDRIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z5</accession>
    <name>F0T9Z5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetate/CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644169.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0567"/>
    <dbReference type="EnsemblBacteria" id="ADZ08818">
        <property type="protein sequence ID" value="ADZ08818"/>
        <property type="gene ID" value="Metbo_0567"/>
    </dbReference>
    <dbReference type="GeneID" id="10277012"/>
    <dbReference type="KEGG" id="mel:Metbo_0567"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OMA" id="DHWWHDL"/>
    <dbReference type="OrthoDB" id="24323at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="6">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="4">
        <location>
            <begin position="99"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="5">
        <location>
            <begin position="537"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="649" mass="73761" checksum="BAE05C537E0371C7" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA18</accession>
    <name>F0TA18_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644192.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0590"/>
    <dbReference type="EnsemblBacteria" id="ADZ08841">
        <property type="protein sequence ID" value="ADZ08841"/>
        <property type="gene ID" value="Metbo_0590"/>
    </dbReference>
    <dbReference type="GeneID" id="10277035"/>
    <dbReference type="KEGG" id="mel:Metbo_0590"/>
    <dbReference type="eggNOG" id="arCOG04897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="OMA" id="WMGQNTN"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="33"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="390" mass="44140" checksum="C13716AA18EEE735" modified="2011-05-03" version="1">MISPKKYARPAKSIPKGYKTANDFFDYVFNDEDMVWMGQNTNHLHDENEIMDSMMQSIKKRDYNKYPPPEGFPELKELILEDLGLQSEFDILVTAGGTESLYLCCNDILAPENNTITCDPGYLIIDNFASRFGDHVKSVPIYNKECGYKLTPKLVRENMDANTKLISLVDPLNPLGSSYNKEELKDFADLAEDNDIYLLHDITYRDFAEEHHLMAKYAPEHTLTVYSFSKIYGMAGMRIGALIGIPELIKSIKTIVINDLGTNLVAQRGALAAVKSKKQWLGRITDITRSNQKIIKDAVDQVSGAFIPVYPSQGNMLAIDLKNTGVTPLEISEYLLERKIFTREGSYTSSRFGERYLRVSFSIPPEDVHYFAKTFLEGMESIKRSKLCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA21</accession>
    <name>F0TA21_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0593</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644195.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0593"/>
    <dbReference type="EnsemblBacteria" id="ADZ08844">
        <property type="protein sequence ID" value="ADZ08844"/>
        <property type="gene ID" value="Metbo_0593"/>
    </dbReference>
    <dbReference type="GeneID" id="10277038"/>
    <dbReference type="KEGG" id="mel:Metbo_0593"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="OMA" id="ELPVAPC"/>
    <dbReference type="OrthoDB" id="127272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="6">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="7307" checksum="159F15D68F960F3B" modified="2011-05-03" version="1">MTELPVAPVGRIIKNAGAQRISDDARDELAKVLEEAGEEIALEAVKLAKHAGRKTVKASDIDLAVKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA29</accession>
    <name>F0TA29_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ordered locus">Metbo_0601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644203.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0601"/>
    <dbReference type="EnsemblBacteria" id="ADZ08852">
        <property type="protein sequence ID" value="ADZ08852"/>
        <property type="gene ID" value="Metbo_0601"/>
    </dbReference>
    <dbReference type="GeneID" id="10277046"/>
    <dbReference type="KEGG" id="mel:Metbo_0601"/>
    <dbReference type="eggNOG" id="arCOG04077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="OMA" id="VCRECHR"/>
    <dbReference type="OrthoDB" id="119723at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="PTHR40704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4" evidence="3">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="6796" checksum="E483ADCEDEC17848" modified="2011-05-03" version="1">MVLKACTRCHRLMEEDRCAICNIASSKNWSGFLIVIDPDDSDIAKELNITLPGEYALRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA56</accession>
    <name>F0TA56_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1784"/>
    <dbReference type="EnsemblBacteria" id="ADZ10006">
        <property type="protein sequence ID" value="ADZ10006"/>
        <property type="gene ID" value="Metbo_1784"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1784"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629484_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL_N" evidence="1">
        <location>
            <begin position="85"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="452" mass="47297" checksum="7125821D5C517695" modified="2011-05-03" version="1" precursor="true">MSKYDSLFHLGVVKIKQKLALTILLLIGLSMTLNLGTSFAANSSSTSNVQIKNTTNTTVKTTATSTKTVSTAKTVGTTSTKTVRVLIYNGNGAITSCVTGIKTALTSVNNNNLVPGYKFTYATSTTISSSILSNYDLLVMPGGSSGRTYINSLSSSAKSAIKNFVYTGHGFLGICAGAYAGSNYVDSMYSGLGVAPHVRSKSVIHEGTLPVTMTSSGSSLLGFSGTLNLAHYNGPAMYASGGTITTFATYADSSTGYKGYGAIVGDTYGSGRTVLSGPHPELAPQNPTLLAKMMIWASNVGSSTSTSFTMSQINTAAKSVKTYIETNKKMPSYVTISGTQVTMAQFLKLLTSDLINVNSGSSSAITLTSTTAPGSTVNTLKAGNIQKSEYLKIANSINSFINTNNRAPSYASSTLGKIGFGSMVYMFSKIMVYYASNGRLPSYVSMTSSSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA86</accession>
    <name>F0TA86_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645387.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1814"/>
    <dbReference type="EnsemblBacteria" id="ADZ10036">
        <property type="protein sequence ID" value="ADZ10036"/>
        <property type="gene ID" value="Metbo_1814"/>
    </dbReference>
    <dbReference type="GeneID" id="10278271"/>
    <dbReference type="KEGG" id="mel:Metbo_1814"/>
    <dbReference type="eggNOG" id="arCOG00512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067890_2_2_2"/>
    <dbReference type="OMA" id="SYFRSVI"/>
    <dbReference type="OrthoDB" id="110669at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="20412" checksum="5C31E2DD7DDA5592" modified="2011-05-03" version="1">MNKIRYVQRKCSDKEKINDFLTSSRVGVLGMNDENSPYAVPLNYVWYHDSIFFHGAGSGRKELILSKEPKVCFTVFEEFGTVLDPMPCHADTSYMSVMLFGQIQRVMDFEESANILDQLVEKYTPGYYKKGLSAKMMEKYLSDLDGNPTSVYRLKPTDITAKENVVEEDELFKSGDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA8</accession>
    <name>F0TAA8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl24e</name>
        <name evidence="6" type="ordered locus">Metbo_0609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644211.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0609"/>
    <dbReference type="EnsemblBacteria" id="ADZ08860">
        <property type="protein sequence ID" value="ADZ08860"/>
        <property type="gene ID" value="Metbo_0609"/>
    </dbReference>
    <dbReference type="GeneID" id="10277054"/>
    <dbReference type="KEGG" id="mel:Metbo_0609"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="OMA" id="GTGKMYV"/>
    <dbReference type="OrthoDB" id="125162at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023438">
        <property type="entry name" value="Ribosomal_L24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_L24e-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="6182" checksum="86F6EF848F13058F" modified="2011-05-03" version="1">MRTCSFCKEEIEEGTGKMYVKKDGTVYFFCSSKCEKNQIGLGRVPRKVKWVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD3</accession>
    <name>F0TAD3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamate synthase alpha subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644236.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0634"/>
    <dbReference type="EnsemblBacteria" id="ADZ08885">
        <property type="protein sequence ID" value="ADZ08885"/>
        <property type="gene ID" value="Metbo_0634"/>
    </dbReference>
    <dbReference type="GeneID" id="10277079"/>
    <dbReference type="KEGG" id="mel:Metbo_0634"/>
    <dbReference type="eggNOG" id="arCOG00098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867712_0_0_2"/>
    <dbReference type="OMA" id="FTYNFIW"/>
    <dbReference type="OrthoDB" id="67919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="308" mass="33537" checksum="DC9EE6C9607D43C4" modified="2011-05-03" version="1">MFEIFKKKSEPSEDILEIDLEEPVDCICDFTFNFHWQHKGEKLDPSWKVPNNEVSFGDLVEHLKQGNNIKIHGDVGHRLCSSMGTDLVYFGGNGSDLNVGDVYVDGNVDSRMGISMTRGRIYIKGTIKEPVGNVVQVKSDVKGYKKFRSITDIMSNGLENDSPLGFQVNGNQMIIDDGMVKDTVGARLDVDGKITINGNVDLSTGILMRKGSVRVNGDAGKNTGALLNGGTVIINGTTDDFTGIDMMHGNIAVNGNAGKFMAANKKDGNIFAHSGNPIPPTTEKSVTNSDQQFLIGLGFNPRKFKKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK1</accession>
    <name>F0TAK1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645424.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1851"/>
    <dbReference type="EnsemblBacteria" id="ADZ10073">
        <property type="protein sequence ID" value="ADZ10073"/>
        <property type="gene ID" value="Metbo_1851"/>
    </dbReference>
    <dbReference type="GeneID" id="10278308"/>
    <dbReference type="KEGG" id="mel:Metbo_1851"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_4_2"/>
    <dbReference type="OMA" id="THMDAPR"/>
    <dbReference type="OrthoDB" id="93116at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="PTHR31118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="25347" checksum="84C14231BAE9815B" modified="2011-05-03" version="1">MKTVILSYPIENNSPHYIGTTEPSLEPLTQIKNGDDYNTYKITVGNHSGTHIDAPKHFVDTGRSILDYSPEELSFKNPIVLDCPKNPYELIEIKDISDADFDKCECLLFKTGFGKFRETDLQKYLTKSPGIAVETVDWIRETHPQIRCLGIDTISMSRYRDAENAKQTHITGFKTSPNYGEPLLFIEDMNLDMDEELTIKEVMVVPWQIKGVDSTPCTVIGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM4</accession>
    <name>F0TAM4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645447.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1875"/>
    <dbReference type="EnsemblBacteria" id="ADZ10096">
        <property type="protein sequence ID" value="ADZ10096"/>
        <property type="gene ID" value="Metbo_1875"/>
    </dbReference>
    <dbReference type="GeneID" id="10278333"/>
    <dbReference type="KEGG" id="mel:Metbo_1875"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OMA" id="DERIHEM"/>
    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="28"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="21486" checksum="9EB6A66A52E057F0" modified="2011-05-03" version="1">MWDFSKLHEKFHEKIGEMQRLGGMRIWIIHVLDEGPKNGVEIMDAIEEHHKMMEQRHMTDERFRRHMAKMKRPSPGSVYPMLKKMVEEDLIIKLEDGKYKLTEKGQKIASSFFGRFRPKEANMDKCEMVVENVLAEIEGHVAFLESIKPEKLEEYCPRINALNKKFEGIMNSLNNPEEEDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ6</accession>
    <name>F0TAQ6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleotide binding protein PINc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0681</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644283.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0681"/>
    <dbReference type="EnsemblBacteria" id="ADZ08932">
        <property type="protein sequence ID" value="ADZ08932"/>
        <property type="gene ID" value="Metbo_0681"/>
    </dbReference>
    <dbReference type="GeneID" id="10277126"/>
    <dbReference type="KEGG" id="mel:Metbo_0681"/>
    <dbReference type="eggNOG" id="arCOG00721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109674_0_0_2"/>
    <dbReference type="OMA" id="GCGREFD"/>
    <dbReference type="OrthoDB" id="102178at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="PTHR12814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="4">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="18447" checksum="444F467C9CF0AB19" modified="2011-05-03" version="1">MNHGVYVLDASAIIGGFISKKDNFVTASVVNEIKDLKSKISLEAAIEDGSIKIIEPDASDLREVGKVIKESGDILRLSGVDIKLVALGYKLKRESLNPTVVTDDYSMQNVLKIIGIPYRSVLTNGINEVYGWIKICKGCKTKYPPEYESGECEICGTRIIKRRIKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT6</accession>
    <name>F0TAT6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator, GntR family</fullName>
            <ecNumber evidence="2">2.6.1.57</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.57" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644313.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0711"/>
    <dbReference type="EnsemblBacteria" id="ADZ08962">
        <property type="protein sequence ID" value="ADZ08962"/>
        <property type="gene ID" value="Metbo_0711"/>
    </dbReference>
    <dbReference type="GeneID" id="10277158"/>
    <dbReference type="KEGG" id="mel:Metbo_0711"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="OMA" id="MRLNFTY"/>
    <dbReference type="OrthoDB" id="51060at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080130">
        <property type="term" value="F:L-phenylalanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="55"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="392" mass="44522" checksum="9EE299456C24238A" modified="2011-05-03" version="1">MNNLYANRMSKIPRSFVREILKVADEPDIISFAGGLPNPDTFPEEEFAESTSYILENSGAEALQYSTTEGYEPLRKLVSERYSKNGLSVDPKNILITNGSQQGLDLVAKVFLNKDDTVLVEKPTYLAALQAFGLFEPNFKSIPLLNDGVDVDVLRSQIEEDSPKLFYSIPNFQNPTGISYSLEKRKIIGKLLNQNETILVEDNPYGEIRFMGENLPPIKTFTDNSILLGSFSKVVAPGIRLGWIVAEDETMHHLVTAKQASDLHSNFFSQMIVHHYLTKYDVEEHLSMIRQMYKDQRDFMVEMIKLHFPDDVKYTQPEGGMFLWVTLPGDMAAMELFERTMEDKVAFVPGEAFYSDDPQKNTLRLNFSNSNNTKIEEGIKKMGNAIRELKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU7</accession>
    <name>F0TAU7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644324.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0722"/>
    <dbReference type="EnsemblBacteria" id="ADZ08973">
        <property type="protein sequence ID" value="ADZ08973"/>
        <property type="gene ID" value="Metbo_0722"/>
    </dbReference>
    <dbReference type="GeneID" id="10277169"/>
    <dbReference type="KEGG" id="mel:Metbo_0722"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088541_1_0_2"/>
    <dbReference type="OMA" id="QTHLRFF"/>
    <dbReference type="OrthoDB" id="120554at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="24632" checksum="1880669799096139" modified="2011-05-03" version="1">MSKYCQFNTNPYGVHQNIISVVGQNKKVLDVGCSEGILTKKMKQNNCDVTGIELDEEASKIAEEFCNELIIGDVEFIQLDTKYEKYFDIILFADILEHLKEPSEVLTRFKKYLNDEGIIIISVPNIANWRKRIQLLFGKFDYQEYGILDNTHIKFFTEKSIKEIIDDAGYKIVKFDLTVGDLNRFSRFFHSVGMIWPNLFAFQFLIVAKIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV8</accession>
    <name>F0TAV8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644335.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08984">
        <property type="protein sequence ID" value="ADZ08984"/>
        <property type="gene ID" value="Metbo_0733"/>
    </dbReference>
    <dbReference type="GeneID" id="10277180"/>
    <dbReference type="KEGG" id="mel:Metbo_0733"/>
    <dbReference type="eggNOG" id="arCOG09726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197250_1_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="58" mass="6266" checksum="4DB8B82A0BEB6BCD" modified="2011-05-03" version="1">MNIINDCSGQGAAEYILLFGGVIVIAVAVLVIYKDYFNPGTQAFRASQDLNKIRSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB91</accession>
    <name>F0TB91_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl6</name>
        <name evidence="5" type="ordered locus">Metbo_0791</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644393.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0791"/>
    <dbReference type="EnsemblBacteria" id="ADZ09042">
        <property type="protein sequence ID" value="ADZ09042"/>
        <property type="gene ID" value="Metbo_0791"/>
    </dbReference>
    <dbReference type="GeneID" id="10277239"/>
    <dbReference type="KEGG" id="mel:Metbo_0791"/>
    <dbReference type="eggNOG" id="arCOG04090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OMA" id="VFQDGIY"/>
    <dbReference type="OrthoDB" id="80629at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_L6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_L6_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_L6P_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="4">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="4">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="19712" checksum="C3834BCDC14527F3" modified="2011-05-03" version="1">MAQVSILREEIPIPEGITVTVDEAVTIKGSKGTLTRNFNNKKAITISVEGDNVVVEARFPKKKEKAMLGTIRSHINNMIIGLTDGFSYKMKIVYAHFPMTVKKTGDKITIENFLGERHPRSAKIVGDADVKIKGDEVIVSGINKEHVGQTMANMEQATKIKGRDPRVFQDGIYLVSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA0</accession>
    <name>F0TBA0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644402.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0800"/>
    <dbReference type="EnsemblBacteria" id="ADZ09051">
        <property type="protein sequence ID" value="ADZ09051"/>
        <property type="gene ID" value="Metbo_0800"/>
    </dbReference>
    <dbReference type="GeneID" id="10277248"/>
    <dbReference type="KEGG" id="mel:Metbo_0800"/>
    <dbReference type="eggNOG" id="arCOG02673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121274_0_0_2"/>
    <dbReference type="OMA" id="LGWYILC"/>
    <dbReference type="OrthoDB" id="94214at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="PTHR42198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="22060" checksum="54339FFB937C9C65" modified="2011-05-03" version="1">MDFSILNPILNPIFNPLLHLVSVPNNPYFGAIIGVFLIATFVAFVITVANKLLVDQDRLQFLQGEMKGFQSEMMKAQKSGDPKAMAEMQKKQAKFMDLQKEMMFNSFKPMIVTFVPIILIFYWMAGNPLINHLYLNLPSFAYYVLLVPIFHTFYHQSAGVPVMAVEWLGWYILCSFSMSFVWRKLLGLKGGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH1</accession>
    <name>F0TBH1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645591.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10240">
        <property type="protein sequence ID" value="ADZ10240"/>
        <property type="gene ID" value="Metbo_2021"/>
    </dbReference>
    <dbReference type="GeneID" id="10278481"/>
    <dbReference type="KEGG" id="mel:Metbo_2021"/>
    <dbReference type="HOGENOM" id="CLU_2103519_0_0_2"/>
    <dbReference type="OrthoDB" id="131686at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="12285" checksum="D60336492BF03A1F" modified="2011-05-03" version="1" precursor="true">MGFNSKNLVVVVFLVAVLVAVSGCTSNTNMTNNSTNNTNNVVVNNSTGHNTTNYISASKAKEIAQQYTAMGVTLGEPTLTTYNGVKVWKVPVNTVGTEEHVDFIYINAVSGKKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK9</accession>
    <name>F0TBK9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-4 C11-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0836</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.133" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644437.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0836"/>
    <dbReference type="EnsemblBacteria" id="ADZ09086">
        <property type="protein sequence ID" value="ADZ09086"/>
        <property type="gene ID" value="Metbo_0836"/>
    </dbReference>
    <dbReference type="GeneID" id="10277285"/>
    <dbReference type="KEGG" id="mel:Metbo_0836"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="OMA" id="WSAMGEQ"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="27422" checksum="3F38C4E00483D8A3" modified="2011-05-03" version="1">MAGKVIFIGGGPGDPELLTLKAYNIIKKADVIIYAGSLVNRDVLNSARDDCLIRNSASMNLDEILDLMETSVAEDKLVARVHTGDPAIYGAIGEQIEGLKSKNIDFDIIPGVSSFFAAAAALETELTLPEVSQTVIITRPEGRTPKPSKEAISKLAEHEATMCIFLGVHMIDDVVKDLLTHYTLETPVAVVQKASWPDEKIVKGTLEDIGVKVAESGIKKTAMIVVGDVLGTENITPSKLYDRKFTHEYRTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN0</accession>
    <name>F0TBN0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0857"/>
    <dbReference type="EnsemblBacteria" id="ADZ09107">
        <property type="protein sequence ID" value="ADZ09107"/>
        <property type="gene ID" value="Metbo_0857"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0857"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063387_0_0_2"/>
    <dbReference type="OMA" id="NTFLEAW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="161"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="343" mass="39711" checksum="35C416BD17FEF54A" modified="2011-05-03" version="1">MVGGAELQYTMLSEQLKNHFNVSFIVFDHGQDSHIKVNNIDLFKACTFNEFSQKKYFKIIIATFKALNYSGADIFMSRSGRIFPAIIAFYCFITGKKFIYSIASDMDVDITNFGYIELILYRFILKRANMVTSQSHFQKKLLKKNFGRESYVIKNVYSLKKRKSEKNVEKSILWVSTIKKGWKNPDLYLELARKIPNIPFVMIGGPSNDDPNYYQEIKMKAKNISNLDFKGYIPYNKIGSYFTDASIFVNTSSVEGFPNTFLQAWESYTPVVSLYIDPDEVICNNNLGFHSKEFEKMVKDVKILINNNELRNQMGQNGRNFIEKEHNINNISCKLIKLLNSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN2</accession>
    <name>F0TBN2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644460.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0859"/>
    <dbReference type="EnsemblBacteria" id="ADZ09109">
        <property type="protein sequence ID" value="ADZ09109"/>
        <property type="gene ID" value="Metbo_0859"/>
    </dbReference>
    <dbReference type="GeneID" id="10277308"/>
    <dbReference type="KEGG" id="mel:Metbo_0859"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_786688_0_0_2"/>
    <dbReference type="OMA" id="WENSAYC"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="61"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="157"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="352" mass="40912" checksum="89FE2E7F353C4EB7" modified="2011-05-03" version="1">MEIFNYYLISGFIENKYKIFLFSNCESNWNTEYLSLINLKERQSLNNVISVIYDILINLFKLRKEIDVIHVPYASNSPIIYPVLIIYLFFGIPYNITIHGGGLKSWKPKFVHKYFFKKAECIIAVSKPVKYEYKKRIDKSICVIPPLIPFTKSSFKKNELRKRYQIDTMSKILISIGSIKNIKRCDILLNAFLAFEKEYIKKNKLLLIFVGEGPQKENLLRKVIENGFSKYVIFFGELPYSQIKDIYELADIYVISSIFESKSISLLEAMFNGLTIIGANVSDINNTITDGKTGLLFEVENSKDLKDKMIQVIENDLYAKKLGLNAEKNFYDNFSFDKVLSTYLNVFKKTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN7</accession>
    <name>F0TBN7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0864</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644465.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0864"/>
    <dbReference type="EnsemblBacteria" id="ADZ09114">
        <property type="protein sequence ID" value="ADZ09114"/>
        <property type="gene ID" value="Metbo_0864"/>
    </dbReference>
    <dbReference type="GeneID" id="10277313"/>
    <dbReference type="KEGG" id="mel:Metbo_0864"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_6_2_2"/>
    <dbReference type="OMA" id="IYYRTDM"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="329"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="478" mass="54530" checksum="E4A47C24868926AA" modified="2011-05-03" version="1">MNSVQKITKNIGITGISQIILAISSFILMIYIARYFGTSEFGEYNFAISFTMLFSIITDLGISQMIIREIARDKDSTNKYMSNALIIKLVMSIVTIGLIAVTINLLNYPPLMVTIVYLFGIYTIFTSFSQTFVSVFQAFEKMEYSNLILILGNIILIPIAFYVLLNNYGLIQLAYVYVLSGFFNALLGFFIFRQKITKLTISLDLNFCKKMIIESLPFGLNAIFANFFFRFDTILLSIIKDNYAVGIYAAAYNPLLALGNILTNMFATTIYPVMSRYYVHSEDSLKRMAIMSSKYMIIIGLPITIGCLVLSKQLIELFYGIKYFDSILAFQILTFFIPIRLISNITGTLLTSINKQKIRTFCVFLSVIFNIISNLILIPYFSFLGASIATVLSELFLYVSFLYFTNKYYHNIIVIKNFVNPIIASFIMGGFAFYFKTFSIILIIFASSIIYFLILILLGTFKSEDDKFIINQLKEVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN9</accession>
    <name>F0TBN9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644467.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0866"/>
    <dbReference type="EnsemblBacteria" id="ADZ09116">
        <property type="protein sequence ID" value="ADZ09116"/>
        <property type="gene ID" value="Metbo_0866"/>
    </dbReference>
    <dbReference type="GeneID" id="10277315"/>
    <dbReference type="KEGG" id="mel:Metbo_0866"/>
    <dbReference type="eggNOG" id="arCOG00252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_1_2"/>
    <dbReference type="OMA" id="CNSQPYS"/>
    <dbReference type="OrthoDB" id="41144at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="10">
        <location>
            <begin position="330"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="448" mass="49568" checksum="1ADC5BC394FBF881" modified="2011-05-03" version="1">MGNLLERIQKRTAIIAIVGLGYVGLPTAIFFAENGFKVIGVEKDEVKLTKIKKGISTIGELNLDMRLSKVVNEKKLIGTADLKWATEQSDIIISIVPTPVDEFKDPDLTPIKSSGEQIAKGLAEGKLVILESTVYPGVTEECLQPILEQSGLKAGTDFGLAYCPERYNPGDVDHGIEKVSRVVGGITPEWAEVTRELYKIIINEDVKVLNNIKTTEAAKVIENTQRDLNIALMNELAMIFERLDIDVMDVIEGASTKWNFNVYYPGAGVGGHCLPVDPYYLVKKAKELGYHSKVIAAGRSINDNMPLHVMNLVQDALNEQEKSIKNSKIVVLGFSYKENVGDPRESPAKTLTKNLVWKEAEVVIVDPYIEEINEKFGVLNNKFSDAINGADALVLMTAHDDFKSIDFNEVLKSMNTPIIVDGRRIYDPDELRDMGFHYKGVGAVNTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ2</accession>
    <name>F0TBQ2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644480.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09129">
        <property type="protein sequence ID" value="ADZ09129"/>
        <property type="gene ID" value="Metbo_0880"/>
    </dbReference>
    <dbReference type="GeneID" id="10277329"/>
    <dbReference type="KEGG" id="mel:Metbo_0880"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="OMA" id="MEREFVT"/>
    <dbReference type="OrthoDB" id="109435at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17439" checksum="26E02BC8F7DC421A" modified="2011-05-03" version="1">MDKELLIQVTEEMYLLFPLFKNKLFKHKKSLKHNSMPHSYFHVLKVLKKRGPLPMSEIGRKVCISKSNMTSLIDKLVQNGLTERIPDQNDRRVINIALTEKGNQLIEDWHSHSNQQIMKKLSSISDEDMETLHKSLKNIRTILNKMDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ4</accession>
    <name>F0TBQ4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644482.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0882"/>
    <dbReference type="EnsemblBacteria" id="ADZ09131">
        <property type="protein sequence ID" value="ADZ09131"/>
        <property type="gene ID" value="Metbo_0882"/>
    </dbReference>
    <dbReference type="GeneID" id="10277331"/>
    <dbReference type="KEGG" id="mel:Metbo_0882"/>
    <dbReference type="eggNOG" id="arCOG02487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_249127_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032109">
        <property type="entry name" value="Big_3_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16640">
        <property type="entry name" value="Big_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NosD" evidence="1">
        <location>
            <begin position="211"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="NosD" evidence="1">
        <location>
            <begin position="425"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Big_3_5" evidence="2">
        <location>
            <begin position="930"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1499" mass="159687" checksum="8439CE627B3A2D96" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU3</accession>
    <name>F0TBU3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645636.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10285">
        <property type="protein sequence ID" value="ADZ10285"/>
        <property type="gene ID" value="Metbo_2066"/>
    </dbReference>
    <dbReference type="GeneID" id="10278528"/>
    <dbReference type="KEGG" id="mel:Metbo_2066"/>
    <dbReference type="eggNOG" id="arCOG04894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087505_0_0_2"/>
    <dbReference type="OMA" id="PEFYFEI"/>
    <dbReference type="OrthoDB" id="75539at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019218">
        <property type="entry name" value="DUF2119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09892">
        <property type="entry name" value="DUF2119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005919">
        <property type="entry name" value="UCP005919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="202" mass="23352" checksum="B1EC0773BB11A5AC" modified="2011-05-03" version="1">MQFIKKINGNHGPTRVFVGGVHGKEGLTTIEPLKQLSQMDVLNGKLILFNCPESKYISTLDPDYYQTKMGQTIIKIIEKYRPEIYVELHCYKPSSYQKLINLNRKDEAGVPPLIELEEGVLISSVAPYLRTELFKRDDVCITLEMPCNPSENSKKVYFEIMQQIATSNNRKEFEAKLRVKYPKQVERARIYAAEFFGEYPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV3</accession>
    <name>F0TBV3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rad50</name>
        <name evidence="8" type="ordered locus">Metbo_2076</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645646.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2076"/>
    <dbReference type="EnsemblBacteria" id="ADZ10295">
        <property type="protein sequence ID" value="ADZ10295"/>
        <property type="gene ID" value="Metbo_2076"/>
    </dbReference>
    <dbReference type="GeneID" id="10278538"/>
    <dbReference type="KEGG" id="mel:Metbo_2076"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="OMA" id="FELPYSH"/>
    <dbReference type="OrthoDB" id="1771at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA_23" evidence="7">
        <location>
            <begin position="6"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="6">
        <location>
            <begin position="439"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="162"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="299"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="707"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="811"/>
            <end position="816"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="900" mass="102539" checksum="1A2E960ADC42DF8C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC15</accession>
    <name>F0TC15_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644517.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0917"/>
    <dbReference type="EnsemblBacteria" id="ADZ09166">
        <property type="protein sequence ID" value="ADZ09166"/>
        <property type="gene ID" value="Metbo_0917"/>
    </dbReference>
    <dbReference type="GeneID" id="10277366"/>
    <dbReference type="KEGG" id="mel:Metbo_0917"/>
    <dbReference type="eggNOG" id="arCOG01384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07799">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015701_0_0_2"/>
    <dbReference type="OMA" id="KIAWPRN"/>
    <dbReference type="OrthoDB" id="3427at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019290">
        <property type="entry name" value="GlycosylTrfase-like_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10111">
        <property type="entry name" value="Glyco_tranf_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_tranf_2_2" evidence="1">
        <location>
            <begin position="105"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="662" mass="76325" checksum="6D06DCF2F5FA5F35" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB3</accession>
    <name>F0TCB3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FtsX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_2165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645731.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2165"/>
    <dbReference type="EnsemblBacteria" id="ADZ10380">
        <property type="protein sequence ID" value="ADZ10380"/>
        <property type="gene ID" value="Metbo_2165"/>
    </dbReference>
    <dbReference type="GeneID" id="10278628"/>
    <dbReference type="KEGG" id="mel:Metbo_2165"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192641_0_0_2"/>
    <dbReference type="OMA" id="MNINLLE"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="128"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="24103" checksum="01B1E6B9DA19B05E" modified="2011-05-03" version="1" precursor="true">MNIYRLSMKNLRRNKMRNFFAVVRISFGVLILLVLLSSGIGLNTFLKQANSFHMNDNVNNSDLIISTVTNQLNAILGVNSTTSVIGSKIDTLINNAVYILDGLASIVFLVGILDIMNTMGFNLNERNREIGILKTMGFSEKELLISMSLEAGLIGFFGGIGGVILGSLGISIVAMFIGIPISILIPLWLIPSVIVLTTVLASILGLVPGWFASYRNIEEVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB8</accession>
    <name>F0TCB8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S8e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps8e</name>
        <name evidence="6" type="ordered locus">Metbo_2170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645736.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2170"/>
    <dbReference type="EnsemblBacteria" id="ADZ10385">
        <property type="protein sequence ID" value="ADZ10385"/>
        <property type="gene ID" value="Metbo_2170"/>
    </dbReference>
    <dbReference type="GeneID" id="10278633"/>
    <dbReference type="KEGG" id="mel:Metbo_2170"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OMA" id="NPNYVRR"/>
    <dbReference type="OrthoDB" id="116294at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_S8e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_S8e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_S8e/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="PTHR10394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="13941" checksum="DCDFC119314FDA05" modified="2011-05-03" version="1">MAIWQGKSLRKPTGARSKSNRNKRNMEFGRDPAETKIGDRKVKKIRTKGGNTKTRLSNDTNINVVDPKTNKVQVAEISTVVENTANTHFVRRNIITKGAVVETNLGRVKVTSRPGQDGMINGVLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC7</accession>
    <name>F0TCC7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdB</name>
        <name evidence="9" type="ordered locus">Metbo_2179</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645745.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2179"/>
    <dbReference type="EnsemblBacteria" id="ADZ10394">
        <property type="protein sequence ID" value="ADZ10394"/>
        <property type="gene ID" value="Metbo_2179"/>
    </dbReference>
    <dbReference type="GeneID" id="10278642"/>
    <dbReference type="KEGG" id="mel:Metbo_2179"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_0_1_2"/>
    <dbReference type="OMA" id="PPQVQAM"/>
    <dbReference type="OrthoDB" id="128807at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="28"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="13873" checksum="D939D1F841C7228A" modified="2011-05-03" version="1">MELPKNIQHQIAQFQQAQQQAQAITMQKQSVDMQIKETEKALEELKKTDEGSDVYKTAGNLLIKVNRDEINEELEEKIETLKLREKTVSRQEERIMAKLQEMQNSLQEAMQGSGMNPGLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF5</accession>
    <name>F0TCF5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2208</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645773.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2208"/>
    <dbReference type="EnsemblBacteria" id="ADZ10422">
        <property type="protein sequence ID" value="ADZ10422"/>
        <property type="gene ID" value="Metbo_2208"/>
    </dbReference>
    <dbReference type="GeneID" id="10278672"/>
    <dbReference type="KEGG" id="mel:Metbo_2208"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="OMA" id="RWTADQM"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="133"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23038" checksum="AAE9D878B1E72A02" modified="2011-05-03" version="1">MYVVIMGGGRVGLALASSLVSVGNDVTLIENNLDLCSNAASELDAMVICGNGTDIKTLKEANIADADVFVAATGNDEANLLSCILVKDYKIPKVIARVSNPDHDDAFRKVGIDVVVSPELTAAGYLEKLITRPKIADLIVIGKGNAELLDISVNNKKVIGKKIGEVSPTDNYIIAAIYKNGEIKIPKEGMMLKEGDKISILAKTHAIKEVTKKFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF7</accession>
    <name>F0TCF7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2210</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="8">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2210"/>
    <dbReference type="EnsemblBacteria" id="ADZ10424">
        <property type="protein sequence ID" value="ADZ10424"/>
        <property type="gene ID" value="Metbo_2210"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2210"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="AHNCLET"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHH_1" evidence="9">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="NikR_C" evidence="10">
        <location>
            <begin position="53"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="16013" checksum="AEE4D4432199AE44" modified="2011-05-03" version="1">MRISMSLPKKLLNDFDEVLKDRGYQSRSKGIRDALKDYIVRYQWMNEMEGDRIGILAVIYDHHYTGVMEDLTDIQHDYRDYINAVMHVHMTEKYCLEVIVVKGDVKYIRDLTEKIMRLKGVEHVKLTSTASGEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG4</accession>
    <name>F0TCG4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydroxylamine reductase</fullName>
            <ecNumber evidence="6">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hybrid-cluster protein</fullName>
            <shortName evidence="6">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hcp</name>
        <name evidence="7" type="ordered locus">Metbo_0994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="6">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0994"/>
    <dbReference type="EnsemblBacteria" id="ADZ09241">
        <property type="protein sequence ID" value="ADZ09241"/>
        <property type="gene ID" value="Metbo_0994"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0994"/>
    <dbReference type="eggNOG" id="arCOG02430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_0_0_2"/>
    <dbReference type="OMA" id="EINLKCM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
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    </dbReference>
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        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="6">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="434" mass="47971" checksum="EB853354D894D3DF" modified="2011-05-03" version="1">MPEKLDMFCYQCSQTAFETGCTVKGVCGKEATVARLQDNLLFSMKGISAYLYHSRELGYTDPEVDQFLEKGFYSTLTNVNFDPAEFVELAKESGMMNIKAMQLLKKAHIETYGEPTPTEVKVGSVKGKGIIATGHSLKALEELLKQTEGTGINVYTHSELLPAHGYPELRKYKHLKGQIGGPWFDQKKTFSNYPAAIVGTSNCVLIPKDDYKERMFTVGVAQLPGVTHIEDYDFTPAIEMAKSLPDLEDEPRDTVLTTGFGASTILALAGKIKELVLAGKIKHFFVVGGCDSPKPQAKYYTEFVKNLPEDVIVLTLACGKYRFNDMDLGNIEGVPRLIDLGQCNDAIVGIDVVVALSDLFEMEINDLPLTFVLSWMEQKAAAILWSLLALDIQNMYLGPIVPGWVNDEILKVLVDNYGLTPIGDPKEDIKKMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ2</accession>
    <name>F0TCJ2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin B12-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644620.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1022"/>
    <dbReference type="EnsemblBacteria" id="ADZ09269">
        <property type="protein sequence ID" value="ADZ09269"/>
        <property type="gene ID" value="Metbo_1022"/>
    </dbReference>
    <dbReference type="GeneID" id="10277471"/>
    <dbReference type="KEGG" id="mel:Metbo_1022"/>
    <dbReference type="eggNOG" id="arCOG02032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082102_0_0_2"/>
    <dbReference type="OMA" id="GYCADAM"/>
    <dbReference type="OrthoDB" id="54330at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="129"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30355" checksum="D2AD6EEC47A97567" modified="2011-05-03" version="1">MSYDEFESKMDQNFVAVRYNVEVEGAAIKPEEDLDVQNTLPTDEPFRSIALSVLYEDRDSSLANVQKALKDKISPIEIINNGLMKGMDAVNILYTKGFYFLPDLMLAGDGMMEGVKECEKVLGHKSETKGTVVSFVAEGDPHDIGKNMVVMFLRAGGFEAIDMGRDVPIKDVVNAVKEYNPLFMTGTALMTTTMTAFPKIVDEIQKEGLEVPAIGCGGGAVRKDFVESFPMSVYGVEAYHAPKLAEAILKDKKTWKDLRKEYSKIVGEFVSEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ3</accession>
    <name>F0TCJ3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanol:cobalamin methyltransferase, MtaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644621.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1023"/>
    <dbReference type="EnsemblBacteria" id="ADZ09270">
        <property type="protein sequence ID" value="ADZ09270"/>
        <property type="gene ID" value="Metbo_1023"/>
    </dbReference>
    <dbReference type="GeneID" id="10277472"/>
    <dbReference type="KEGG" id="mel:Metbo_1023"/>
    <dbReference type="eggNOG" id="arCOG03330">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_588790_0_0_2"/>
    <dbReference type="OMA" id="CWSNESV"/>
    <dbReference type="OrthoDB" id="11190at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021079">
        <property type="entry name" value="MeOH-cob_MeTrfase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12176">
        <property type="entry name" value="MtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="460" mass="50633" checksum="D8979E51E856EB06" modified="2011-05-03" version="1">MKRFTKMVYKDSDDMVFGHAKFPVSQHWNTQIGAGYVVPEIKVAPVEGSEETKEGMVSECRNITQSACERAVAIGLPAFMVEQEHIFQQTNNPDWTAECTQVQAETLEKYYDEYDIRVSLLATPADIRIEERDGLRGSDYDNKIIESVEASAANGASCVAIETIGGKSVSDYGIARGDPKAILFGIGVLGSIDMEYMWKRIVDVCRKYDCRPAGDTDCAQANTAMFLAGGLLDKDCSHTLAALARAMAGARSLVAVECGAIGPLKDCGYENPIIKAISGVPIATEGKNAVCAHSDLMGNLSAAVTDVWSNESVYHREEMGGTTPEVWLQATGYEAALMNTAIQTGQEKILRDLYTLTDKYRDPQALVLAYDNAYRIGEAIVKYGKDPYLRARAAALEAGAIINEAVESKKMYLTRFERDSLDHALNIFEALPTESEQFTDDCIRKYSRKISEFDPKSYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK5</accession>
    <name>F0TCK5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644633.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1035"/>
    <dbReference type="EnsemblBacteria" id="ADZ09282">
        <property type="protein sequence ID" value="ADZ09282"/>
        <property type="gene ID" value="Metbo_1035"/>
    </dbReference>
    <dbReference type="GeneID" id="10277484"/>
    <dbReference type="KEGG" id="mel:Metbo_1035"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="OMA" id="MAGATNI"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="136"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="23599" checksum="D1A42E1102AF3F9B" modified="2011-05-03" version="1">MYVVIMGAGRVGINLANALIRAGFDVTMIEKDPELCSHASNELDALLICGNGTDLKILDEANVSDADAFVAATGNDEANLLACILVKEFKPKKLIARLSDPAHEDAFHKVGINATVSPELTAASYLEKLIIRPKIADMVVLGKGNAELIDIPVENQDVIGKKIGDMSPNKNYVICAIYKPPNYEIIMPEPNITIEEGDKISILIKTPSIKSVVKKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU5</accession>
    <name>F0TCU5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2271</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(1)-methylpseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methyl-N(3)[(3S)-3-amino-3-carboxypropyl]pseudouridine in 16S/18S rRNA + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645836.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2271"/>
    <dbReference type="EnsemblBacteria" id="ADZ10485">
        <property type="protein sequence ID" value="ADZ10485"/>
        <property type="gene ID" value="Metbo_2271"/>
    </dbReference>
    <dbReference type="GeneID" id="10278737"/>
    <dbReference type="KEGG" id="mel:Metbo_2271"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_2_2"/>
    <dbReference type="OMA" id="DCSWESA"/>
    <dbReference type="OrthoDB" id="113131at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:18S rRNA aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="PTHR20426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RLI" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Ribo_biogen_C" evidence="8">
        <location>
            <begin position="41"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="18869" checksum="404FB4664132A954" modified="2011-05-03" version="1">MKVSVYHAEQCDPKKCTTVKLQRQGKIKVVTKLTMLPKGALVLDPFSAKSVSYEDREIVEKNGIVGLDCSWNRIQKSSIMFKGKKYHRSLPFLVAANPVNYGKPCKLSTAEAIAATFYIVGLKDNAVEIMSQFKWGPHFLKLNHELLEEYSSAKTSAEVVKIQNSFIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T610</accession>
    <name>F0T610_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645868.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2303"/>
    <dbReference type="EnsemblBacteria" id="ADZ10517">
        <property type="protein sequence ID" value="ADZ10517"/>
        <property type="gene ID" value="Metbo_2303"/>
    </dbReference>
    <dbReference type="GeneID" id="10278769"/>
    <dbReference type="KEGG" id="mel:Metbo_2303"/>
    <dbReference type="eggNOG" id="arCOG11014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047003_0_0_2"/>
    <dbReference type="OMA" id="PMRGTLH"/>
    <dbReference type="OrthoDB" id="78407at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009351">
        <property type="entry name" value="YcaQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06224">
        <property type="entry name" value="HTH_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="362" mass="41589" checksum="0FD79C0993001A11" modified="2011-05-03" version="1">MEPNEIAIERLESQHVGSGRIRSPAKLVSWLVAVQAQEYDGAKWSIGLRLRNKTDKYVENALKSKKIVRTWANRGTLHIVNSQDLKWLLNLIGPRLIRRSSRRYRELELDEKTLKTTGKILQEALSGTNGIQRSKLREILIENKISTEGQRFTFILQRASLEGLIHQGISIKNDPIYHSSEDLPSPQLTNEEALMEISKRYFHSHGPATLKDFVWWSGLTVKDAKTGLKSVKSELNRFEMGDKTYWSKISSITHEGQLQTWPNIKILPQYDDYLLAYQDRGASIDGKVKQLLKPKYGRFNQVILVSGKVTGTWKRKITKNNVDFKLNYFRPLSESENKALVSEIMCYSNFIDKTCTGIETNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T620</accession>
    <name>F0T620_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2313"/>
    <dbReference type="EnsemblBacteria" id="ADZ10527">
        <property type="protein sequence ID" value="ADZ10527"/>
        <property type="gene ID" value="Metbo_2313"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2313"/>
    <dbReference type="HOGENOM" id="CLU_3094108_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="51" mass="5771" checksum="854FCBADCC4AF919" modified="2011-05-03" version="1">MSNAMGVIVAYGTSHLTSNQQITELIIILITLLIIILLIRRYDKKITIETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T669</accession>
    <name>F0T669_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2363"/>
    <dbReference type="EnsemblBacteria" id="ADZ10576">
        <property type="protein sequence ID" value="ADZ10576"/>
        <property type="gene ID" value="Metbo_2363"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2363"/>
    <dbReference type="eggNOG" id="arCOG14019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444692_0_0_2"/>
    <dbReference type="OMA" id="MIKAGEN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="192" mass="21759" checksum="4F84CDF21DD84E5A" modified="2011-05-03" version="1">MVLKMNLKDLNAKFSGVKRFFSVLGNLITTYMGIILTIGFLVPWLLGAIKIQDYGEITGFIEVFVLVTATLSLLSFTYIMALSEMHEKVKKSMIKAGENFFMSTVQFIVGLFLLFVMDYITHSFINISNLSFTAVNLGSIIIFIIQIFILLELIFAVSKFFQGVIGVYVSFRAEHLKDSIFYILSWRNAGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T672</accession>
    <name>F0T672_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Geranylgeranyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645930.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2366"/>
    <dbReference type="EnsemblBacteria" id="ADZ10579">
        <property type="protein sequence ID" value="ADZ10579"/>
        <property type="gene ID" value="Metbo_2366"/>
    </dbReference>
    <dbReference type="GeneID" id="10278832"/>
    <dbReference type="KEGG" id="mel:Metbo_2366"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_1_2"/>
    <dbReference type="OMA" id="IGVHMDR"/>
    <dbReference type="OrthoDB" id="115808at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="43455" checksum="FB67D3E3A954CA84" modified="2011-05-03" version="1">MKKYDIAVVGAGPVGSTFARYVADNGLNVVMVEKKREIGIPLQCAGLLGQKIKEINVLPDEYVLNQVRGAYIHSPSNNMMKVGKENVQAYVIDRVGYDKYLANLAVDSGAELLMNHKVIHSDLNGCIETKETKIQADIIVGADGHSSTLSNNFNEKPRSFMAAQYLIETDIPVFDLENVHLHVDDLISPGFLWTIPLTSSTARIGLFADMNYTELNNVLAKLMATEPMFKNARIIQKYQGYIPKYDPKKKIVDEKTILLGDAASQVKPTTGGGLLIGFECAKIAAECATKAVEMEDISYLKEYENQISKRFRNELRVQIEVQKIFESMNNQDLDEMFTKLKQGNAEELISKYGDMDTQSTLIKEMIKNGLLFSIVPKLLTRRIMGLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D4</accession>
    <name>F0T6D4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase</fullName>
            <ecNumber evidence="2">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.98.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644800.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1206"/>
    <dbReference type="EnsemblBacteria" id="ADZ09449">
        <property type="protein sequence ID" value="ADZ09449"/>
        <property type="gene ID" value="Metbo_1206"/>
    </dbReference>
    <dbReference type="GeneID" id="10277656"/>
    <dbReference type="KEGG" id="mel:Metbo_1206"/>
    <dbReference type="eggNOG" id="arCOG00342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_0_0_2"/>
    <dbReference type="OMA" id="YPERRHC"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="162"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="37394" checksum="E9816EE1DD68A478" modified="2011-05-03" version="1">MKFIPDKEILLFKSCLVNVEYPGVESSTCYVFDKIGVEYHVDERQSCCTGLGHYYDLFDQLSTITLAARNFNVAADTGHKNIATMCATCYAILKKSAKILNQNEEAREEVNGILDDSGLEAMEYSSGDMDPAENIFHVAEILFNKREEISKHIKVDLTGFKVASHHACHYCKVHYNDTIEGIRDPNLIDELASACGVETIGWYDHKRVTCGAGFRQRYTNKEASLAVTGEKLQALKDKDVEILLHMCPNCQMQFDRYQPLIGEKLGTEFNIFHLNIAQFIALAMGADPYKVVGIQTHTVPVEPLLNKLKQPFNLKEDQSKRNSLKIDENRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G0</accession>
    <name>F0T6G0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645945.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2381"/>
    <dbReference type="EnsemblBacteria" id="ADZ10594">
        <property type="protein sequence ID" value="ADZ10594"/>
        <property type="gene ID" value="Metbo_2381"/>
    </dbReference>
    <dbReference type="GeneID" id="10278847"/>
    <dbReference type="KEGG" id="mel:Metbo_2381"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_3_0_2"/>
    <dbReference type="OMA" id="WEVGNAY"/>
    <dbReference type="OrthoDB" id="85871at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="210" mass="23442" checksum="8F11BB6797E5920F" modified="2011-05-03" version="1">MKYDFHTHSKYSADGYVKPRELVKAAVKKGLDGIAVTDHGTIKGALEAKKYETNKIKVIIGSEILTNIGEVIGLFLMDEVVSYDFYDVVEDIKSQNGVVVLPHPFDGVRGTSTHPDQKLSRYVDSVEVFNSRCVRNDYNDLAQKYAKKNHLNTIAGSDAHFKNEVGNAGVSTERSDIREAVIKGDLTVFGRRSNILNPVTTKLLKIWRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G1</accession>
    <name>F0T6G1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-phospho-L-seryl-tRNA:Cys-tRNA synthase</fullName>
            <ecNumber evidence="2">2.5.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Sep-tRNA:Cys-tRNA synthase</fullName>
            <shortName evidence="2">SepCysS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25686"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="EC" id="2.5.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer. Interacts with SepRS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SepCysS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.73" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645946.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2382"/>
    <dbReference type="EnsemblBacteria" id="ADZ10595">
        <property type="protein sequence ID" value="ADZ10595"/>
        <property type="gene ID" value="Metbo_2382"/>
    </dbReference>
    <dbReference type="GeneID" id="10278848"/>
    <dbReference type="KEGG" id="mel:Metbo_2382"/>
    <dbReference type="eggNOG" id="arCOG00091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060476_0_0_2"/>
    <dbReference type="OMA" id="HKSMAAS"/>
    <dbReference type="OrthoDB" id="24071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043766">
        <property type="term" value="F:Sep-tRNA:Cys-tRNA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01675">
        <property type="entry name" value="Sep_Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013375">
        <property type="entry name" value="Sep_Cys-tRNA_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF3">
        <property type="entry name" value="PTHR43586:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02539">
        <property type="entry name" value="SepCysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="379" mass="42416" checksum="658C274550666F16" modified="2011-05-03" version="1">MDECQDYGLTRDTERKNLNLNPLQRGGVLPLEARKALYEFSDGYSVCDYCSGRLDEITKPQINGFLEDLAGFINTDFARTVHGAREGKFAVMHALCEPGDTIVIDGNAHYTTHVAAERVGLNMVDVPNTDYPNYEVTPESYKEVLDDLYDQGEEVSLVLLTHVDGNYGNLADARSIGKIAHDAGVPLLLNCAYSMGRLPIDAKALNADFVVGSGHKSMAASGPIGVLGLKEEWADIILKRSDRAQVKELEMLGCTSRGAPVATLMASFPHVIDRVKKWDVEVKETRKFVETLENIEGINQLGKKPKEHDLVRFETPIFDEIAKHHTRRGFFLYEELKKRKIVGIKRGQTKWFKCSVYGFSAEQKEYIAETFVDIASKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M2</accession>
    <name>F0T6M2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643606.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0002"/>
    <dbReference type="EnsemblBacteria" id="ADZ08255">
        <property type="protein sequence ID" value="ADZ08255"/>
        <property type="gene ID" value="Metbo_0002"/>
    </dbReference>
    <dbReference type="GeneID" id="10276424"/>
    <dbReference type="KEGG" id="mel:Metbo_0002"/>
    <dbReference type="eggNOG" id="arCOG10162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080337_0_0_2"/>
    <dbReference type="OMA" id="PYTIMNS"/>
    <dbReference type="OrthoDB" id="95382at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="296" mass="31050" checksum="61166E2A07DE34E0" modified="2011-05-03" version="1">MIEIKDIKSVKIVPFTLMNSSITAVIGLIYGIVFAIIFGVSVGSSLPGITAGMIATIVIGSILAFPTMMFLVSVLESFLVALLYNQLVPRLGAIQLGFEDLKQIQTVPVVPFALMSASIGGILTFLMMLIIGPLFAFGIQSAALAGAAGVPGLSSIGSFGLLWMIILIVGIPIVAFVGIFIATAIAVIIYNLLAPKIGGVELNFSTASGKFFEIESIPVVKFALIITLVLTVIQLILGIIDLIVSIAIGSSAVGALIGLVIQVVTTLVVSFILYAVMAYAYNYLRPKIGGIKLEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M5</accession>
    <name>F0T6M5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643609.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0005"/>
    <dbReference type="EnsemblBacteria" id="ADZ08258">
        <property type="protein sequence ID" value="ADZ08258"/>
        <property type="gene ID" value="Metbo_0005"/>
    </dbReference>
    <dbReference type="GeneID" id="10276427"/>
    <dbReference type="KEGG" id="mel:Metbo_0005"/>
    <dbReference type="eggNOG" id="arCOG10898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147142_0_0_2"/>
    <dbReference type="OrthoDB" id="143410at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="15591" checksum="89E4EBBA18A94BAA" modified="2011-05-03" version="1" precursor="true">MINQKSVLIGTLVSLILLALLSLVLGLLGFYIAVFVGAVITTYLASGKEQLKVIESGLHGIIVGIFTGVIYILFVYAYSGFSKIVAGILIYAALVLIGGFIIVGALGGIIGLLISVKTGHMGVYEGEIEESTEPTKPNDEIESSEKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P2</accession>
    <name>F0T6P2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein Metbo_0022</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643626.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08275">
        <property type="protein sequence ID" value="ADZ08275"/>
        <property type="gene ID" value="Metbo_0022"/>
    </dbReference>
    <dbReference type="GeneID" id="25394711"/>
    <dbReference type="KEGG" id="mel:Metbo_0022"/>
    <dbReference type="eggNOG" id="arCOG03215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089549_2_0_2"/>
    <dbReference type="OMA" id="YDEIMET"/>
    <dbReference type="OrthoDB" id="104709at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14899" checksum="9670401445BCE525" modified="2011-05-03" version="1">MIKPKTDIDDELQGAMSSYNAENFRRLTETHFSGEDEEINTKELTALEESIDHYFELYAPDNIEFREFIKIVSVYLTFIEKRPLHPPGIIFSGGDTVYENKGIYYCTGKKQFKNDDHSLCNFCVCHEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P6</accession>
    <name>F0T6P6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0026"/>
    <dbReference type="EnsemblBacteria" id="ADZ08279">
        <property type="protein sequence ID" value="ADZ08279"/>
        <property type="gene ID" value="Metbo_0026"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0026"/>
    <dbReference type="HOGENOM" id="CLU_3227811_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="5010" checksum="E3AF32A641695B23" modified="2011-05-03" version="1">MYGCEKRLLLFKMKRNGKFNELFYIKVSVPKSKISVIFGLIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R0</accession>
    <name>F0T6R0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIR synthase related protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643644.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0040"/>
    <dbReference type="EnsemblBacteria" id="ADZ08293">
        <property type="protein sequence ID" value="ADZ08293"/>
        <property type="gene ID" value="Metbo_0040"/>
    </dbReference>
    <dbReference type="GeneID" id="10276462"/>
    <dbReference type="KEGG" id="mel:Metbo_0040"/>
    <dbReference type="eggNOG" id="arCOG00637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="OMA" id="IGGDMVI"/>
    <dbReference type="OrthoDB" id="26664at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="131"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="243"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="454" mass="49059" checksum="6E93C66D847C0EAF" modified="2011-05-03" version="1">MDIEGFVRRSIKEEDEETVQKNLVKKILEFKDLDNDKAEKMASAVIEEVKNTLTIDSYPDEFMRDLINYTQSSVSMGDIGVGSRGAGDFFVHRKIAEIVSSAKTSSYINPSAQDDGGVVKSTVDSKDVYITTAVDGIHSRLSEYPFLGGFHVARATLRDVCVMGSNPIAMLSDLHLADDGDVGKLFDFTAGVCAVSELVDVPLVAGSTLRVGGDMVLGDRLVSAVGAVGISEYAPTARKRAESGDVILLTEGSGGGTISTTAIYHGMFDVVQETMDISFIKASEAIFNAGLLPEVHAMTDVTNGGLRGDAHEISQTTGLGLSFSEEKIREMVNPKVLDMLEKLEIDPLGVSVDSLMIIAPEDLATRVKNVVSSAGVKINEIGVVDNSGISKLIKEDGREEELKPLFREAAYTKIKKIVGDIEPEDFDEMKDKVQRAAIESIKKKDKVVKAIQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S8</accession>
    <name>F0T6S8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643662.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0058"/>
    <dbReference type="EnsemblBacteria" id="ADZ08311">
        <property type="protein sequence ID" value="ADZ08311"/>
        <property type="gene ID" value="Metbo_0058"/>
    </dbReference>
    <dbReference type="GeneID" id="10276481"/>
    <dbReference type="KEGG" id="mel:Metbo_0058"/>
    <dbReference type="eggNOG" id="arCOG01895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="PTIDLNP"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="28865" checksum="7E30B62ABE621A62" modified="2011-05-03" version="1">MKLGFSTLALFMNSLEDFLETASIDGFQMIEILCEGPYWPRNVLAFNSNFEIFDSYDIDVYLHSPTIDLNPGSLNPGIREETLKQLKETVNFGVKINCKAITTHPGLIHRLEERVRNFGMEHAINVLTEANNYSEEMGIKFSIENMPNKYAYFCNSADEHKFFVEKCGSYATVDTGHANTSKDVKSFFKLKNIVYYHLNDNDGEKDQHLTLGEGTLDLNLLNGVKNGIIELNNYQNVLKSRDLIRAGWPHDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W0</accession>
    <name>F0T6W0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1238"/>
    <dbReference type="EnsemblBacteria" id="ADZ09480">
        <property type="protein sequence ID" value="ADZ09480"/>
        <property type="gene ID" value="Metbo_1238"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1238"/>
    <dbReference type="eggNOG" id="arCOG01681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_7_3_2"/>
    <dbReference type="OMA" id="YHVEHLR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="40"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="15362" checksum="3F6F7D70034863BD" modified="2011-05-03" version="1">MTLTFIYGDVCIYTTMKIDDMCEIECKHEDTIEEVKSELPADDILLKVSETFKVFGDLTRLKILQALFRRELCVCDLTEVLEANQSTISHQLRILRQNNLVKFRKEGKMAYYSLADDHVEKIIEMGLEHAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W9</accession>
    <name>F0T6W9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644840.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1247"/>
    <dbReference type="EnsemblBacteria" id="ADZ09489">
        <property type="protein sequence ID" value="ADZ09489"/>
        <property type="gene ID" value="Metbo_1247"/>
    </dbReference>
    <dbReference type="GeneID" id="10277698"/>
    <dbReference type="KEGG" id="mel:Metbo_1247"/>
    <dbReference type="eggNOG" id="arCOG02239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299358_0_0_2"/>
    <dbReference type="OrthoDB" id="76647at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="11067" checksum="8BE6C8AD8F499623" modified="2011-05-03" version="1">MENKKYKCKECGFEWQNPKKSYKNCPECQSEDITTIDLTEENQTIIPKPGNMGRGHGNMGGGPPRACKCQKCGYETTKTPGVPCRNQKCPECGTLLCGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X2</accession>
    <name>F0T6X2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heterodisulfide reductase subunit C, HdrC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644843.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1250"/>
    <dbReference type="EnsemblBacteria" id="ADZ09492">
        <property type="protein sequence ID" value="ADZ09492"/>
        <property type="gene ID" value="Metbo_1250"/>
    </dbReference>
    <dbReference type="GeneID" id="10277701"/>
    <dbReference type="KEGG" id="mel:Metbo_1250"/>
    <dbReference type="eggNOG" id="arCOG00965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100474_0_0_2"/>
    <dbReference type="OrthoDB" id="42661at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="21948" checksum="35A59BEDA1AFDEC4" modified="2011-05-03" version="1">MRTIKLKKDPLKLLNDVLKHLKASPELGIFKCVQCGMCSSVCPAAKRTDYDPKEMLGRVLQDDETVVDDDTIWYCFSCYTCNSVCPSGNNACEVNQILRQMAIDKGDVQRIESFTPYGDSFMKKGIGTIPEKYYEDMVSDSGPECMNIKMDIQNIRSSLGLGNYILPDDAVNEIDLILKNSGFMERIEMIKGCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T721</accession>
    <name>F0T721_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="4 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemA</name>
        <name evidence="17" type="ordered locus">Metbo_2442</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AL-21</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 13">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="4 8 13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646006.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2442"/>
    <dbReference type="EnsemblBacteria" id="ADZ10655">
        <property type="protein sequence ID" value="ADZ10655"/>
        <property type="gene ID" value="Metbo_2442"/>
    </dbReference>
    <dbReference type="GeneID" id="10278914"/>
    <dbReference type="KEGG" id="mel:Metbo_2442"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="OMA" id="FAFKCAA"/>
    <dbReference type="OrthoDB" id="48038at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GlutR_N" evidence="16">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="15">
        <location>
            <begin position="150"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="GlutR_dimer" evidence="14">
        <location>
            <begin position="299"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="45"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="168"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ADZ10655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="45411" checksum="4BE3A14B9F45ACC7" modified="2011-05-03" version="1">MILNIRVDHKTADINQMEKSSEQLGIVFKEIITNYDVREYVELKTCNRAEIYLVLNEHPDKRLIENFVTDFDDDAIEHLLRLSSGLESMIIGEDQILGQIKDAKNRSVKDGCCGQMLETIFTKAVHVGQVVRKKTKINEGSLSIGSAAVELAENVHGDLKCKKVLVIGAGKMGTLVAKALVEKKLKAIVVANRTHDRAVELARELGGSAIHFDKLDEAIKDADVVISATGAPHPILTRDKISDVVPSENLSTMVMVDIANPRDIEEEVAELGVKLFNIDDLRSIAQRSREMRQAEACDAEEIVAEEMILLKRSIKQLEVEPIISKIRISAENIRKLETQKAFKMLGDMNGNEKVVDNLTKVVVERIFNDVIRNLKKAAENGDETTIKAAEALFNNQVADAKFKNFDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T736</accession>
    <name>F0T736_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10670">
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        <property type="gene ID" value="Metbo_2457"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2457"/>
    <dbReference type="eggNOG" id="arCOG04298">
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        <property type="component" value="Chromosome"/>
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    </dbReference>
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        <property type="entry name" value="Adenosine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036902">
        <property type="entry name" value="Ta1353-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155">
        <property type="entry name" value="PTHR36155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04008">
        <property type="entry name" value="Adenosine_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF103165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18214" checksum="87A79F9B048562F8" modified="2011-05-03" version="1">MEMKLKMVKVEAPEDCNIILGQSHFIKTVEDLYEAIFNTVPNAKFGIAFSEASGDCLVRTEGNNEELEEFVADTMLEIGCGHSFLIILENAFPINVIHRIKSVPEVVNIYCATANPVEILIVETEQGRGIVGVVDGFKPKGIENEDDKDFRRKFLREIGYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T750</accession>
    <name>F0T750_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646035.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2471"/>
    <dbReference type="EnsemblBacteria" id="ADZ10684">
        <property type="protein sequence ID" value="ADZ10684"/>
        <property type="gene ID" value="Metbo_2471"/>
    </dbReference>
    <dbReference type="GeneID" id="10278945"/>
    <dbReference type="KEGG" id="mel:Metbo_2471"/>
    <dbReference type="eggNOG" id="arCOG03208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158570_1_0_2"/>
    <dbReference type="OMA" id="AVNMIDC"/>
    <dbReference type="OrthoDB" id="109266at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018676">
        <property type="entry name" value="DUF2149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09919">
        <property type="entry name" value="DUF2149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="11487" checksum="9D66EB7B81F1B8DB" modified="2011-05-03" version="1">MIKQKRRARRRAVLSSDDEIDPMIYAVNMIDCMLVLAVGFLIFTIIFMNTNPNEASQANVNIKLGQELNETPQNSSSNSQTGLNPVGTAYEDPTTGKLILVKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T764</accession>
    <name>F0T764_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646049.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2485"/>
    <dbReference type="EnsemblBacteria" id="ADZ10698">
        <property type="protein sequence ID" value="ADZ10698"/>
        <property type="gene ID" value="Metbo_2485"/>
    </dbReference>
    <dbReference type="GeneID" id="10278959"/>
    <dbReference type="KEGG" id="mel:Metbo_2485"/>
    <dbReference type="eggNOG" id="arCOG02710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507744_0_0_2"/>
    <dbReference type="OMA" id="FEDYEPV"/>
    <dbReference type="OrthoDB" id="7749at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1360">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002846">
        <property type="entry name" value="NRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038982">
        <property type="entry name" value="NrpR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036984">
        <property type="entry name" value="NrpR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013668">
        <property type="entry name" value="RNase_R_HTH_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964">
        <property type="entry name" value="PTHR41964"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="HTH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01995">
        <property type="entry name" value="NRD1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_12" evidence="2">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="NRD1_2" evidence="1">
        <location>
            <begin position="84"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="NRD1_2" evidence="1">
        <location>
            <begin position="333"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="564" mass="62270" checksum="57A1E56C75C9F7F5" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B3</accession>
    <name>F0T7B3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argS</name>
        <name evidence="12" type="ordered locus">Metbo_0089</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643693.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0089"/>
    <dbReference type="EnsemblBacteria" id="ADZ08342">
        <property type="protein sequence ID" value="ADZ08342"/>
        <property type="gene ID" value="Metbo_0089"/>
    </dbReference>
    <dbReference type="GeneID" id="10276512"/>
    <dbReference type="KEGG" id="mel:Metbo_0089"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="7046at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
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        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
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        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="11">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="10">
        <location>
            <begin position="447"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ08342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="561" mass="63761" checksum="0628E99AD8FB1BE1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D2</accession>
    <name>F0T7D2_METLA</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643712.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0108"/>
    <dbReference type="EnsemblBacteria" id="ADZ08361">
        <property type="protein sequence ID" value="ADZ08361"/>
        <property type="gene ID" value="Metbo_0108"/>
    </dbReference>
    <dbReference type="GeneID" id="10276533"/>
    <dbReference type="KEGG" id="mel:Metbo_0108"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="OMA" id="VVYPKDA"/>
    <dbReference type="OrthoDB" id="64860at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="PTHR12133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="242" mass="27144" checksum="F4E2C3E463FA3389" modified="2011-05-03" version="1">MRILMDERGKKYMVKDQEFHTDFGYITEEDIVSSKSGDTLKTHLGREFKVIKPNINDYIELMERKCSIILPKDVGIITAITGLGSGYRVLDAGSGSGATAMHFGNIVGDKGEVFSYEIREDFAEIAERNVKGFGLDNVEVKCKDVTEGIEEKDLDLIFLDLPKPWDAADEAMAALKHGGYIATYTPFIEQIQVLQRILKKVGFKEVESFECIFRGIEVTNKGTRPKTRMAGHTGYLSFGRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G5</accession>
    <name>F0T7G5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643745.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0141"/>
    <dbReference type="EnsemblBacteria" id="ADZ08394">
        <property type="protein sequence ID" value="ADZ08394"/>
        <property type="gene ID" value="Metbo_0141"/>
    </dbReference>
    <dbReference type="GeneID" id="10276567"/>
    <dbReference type="KEGG" id="mel:Metbo_0141"/>
    <dbReference type="eggNOG" id="arCOG02469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="OMA" id="IQHYPCR"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="217" mass="24519" checksum="DEA9C4A8078A7261" modified="2011-05-03" version="1">MIKVVIVTDGPYGDRTYDTICEDFETVFIELEQPDSMFMDDVEVPEDSLNELRSADLVISYILHPDLVLEMVEQVHEDVGWIIVAAWRGEGLKNQLEDYGNVTCPDTMCELEENGDPVYDEFVSKFGRPIVEIKLEGNKVVNVDVIRAAPCGSTHYVSREMVGEDVNNLPTRAGLKVQLFPCRAPKLRLFVDECKKGLASNFHSQAFQEAIDKERNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J6</accession>
    <name>F0T7J6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09564">
        <property type="protein sequence ID" value="ADZ09564"/>
        <property type="gene ID" value="Metbo_1322"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1322"/>
    <dbReference type="HOGENOM" id="CLU_2285056_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11319" checksum="F3C12936FEA8058C" modified="2011-05-03" version="1">MKNCLYSNHHSFSVNSLIDVKMSNPPSINFSKSDGAYITKNAPIDAPIMDAMPMGTTRDVIYDFALYILFLVCRVILTIRVGMLISRLIVPANSHQFQILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R4</accession>
    <name>F0T7R4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">secG</name>
        <name evidence="14" type="ordered locus">Metbo_2533</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646097.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2533"/>
    <dbReference type="EnsemblBacteria" id="ADZ10746">
        <property type="protein sequence ID" value="ADZ10746"/>
        <property type="gene ID" value="Metbo_2533"/>
    </dbReference>
    <dbReference type="GeneID" id="10279007"/>
    <dbReference type="KEGG" id="mel:Metbo_2533"/>
    <dbReference type="eggNOG" id="arCOG02957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208205_3_1_2"/>
    <dbReference type="OMA" id="EQVVIMT"/>
    <dbReference type="OrthoDB" id="128075at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="5873" checksum="802EE80E68BF564E" modified="2011-05-03" version="1">MAKKDKQVLPPSGAGLVRYFEDETKGPKLSPEQVVIMTVVLAVFCIALRFTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U3</accession>
    <name>F0T7U3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UbiD family decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643781.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0178"/>
    <dbReference type="EnsemblBacteria" id="ADZ08430">
        <property type="protein sequence ID" value="ADZ08430"/>
        <property type="gene ID" value="Metbo_0178"/>
    </dbReference>
    <dbReference type="GeneID" id="10276604"/>
    <dbReference type="KEGG" id="mel:Metbo_0178"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_5_1_2"/>
    <dbReference type="OMA" id="TEGGCCW"/>
    <dbReference type="OrthoDB" id="35356at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="428" mass="47606" checksum="164BAE2DB8ED44AA" modified="2011-05-03" version="1">MREFLKVLEDDFKVINIDDEVTTHYEVSKIMKDHPKDVLIFNNVKESDMGIISGICNTREKIAKSISVTVPEITARIVEATEKPMPIEVVERISKNFAYSKSADLGELPVPTYYKKDGGAYITAGVIIAKDPETGVRNASIHRMLVKDKDHLGVRIVPRNLYTYYKKAEEMDEPLEVAIAIGMNPASLLASCTSIPITADELEVANTFQNGEMKLVKCETVDLEVPDCEILLEGKILPHERDSEGPFVDLTDTYDVVRQEPIIKLEKMHYKENPLYHAIMPAGNEHKLLQGLPQEPRIYKAVQNTVPTVRNVVLTEGGCCWLHAAISIKKQTPGDGKNVIMAALAAHPSLKHCVVVDEDVNIFDTDDLEYAIATRVRADEDMLIVPGARGSSLDPRATPDGTTTKVGIDATKLLDKLEKFERVSKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V2</accession>
    <name>F0T7V2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Natural resistance-associated macrophage protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643790.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0187"/>
    <dbReference type="EnsemblBacteria" id="ADZ08439">
        <property type="protein sequence ID" value="ADZ08439"/>
        <property type="gene ID" value="Metbo_0187"/>
    </dbReference>
    <dbReference type="GeneID" id="10276613"/>
    <dbReference type="KEGG" id="mel:Metbo_0187"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_1_2"/>
    <dbReference type="OMA" id="IATFVNS"/>
    <dbReference type="OrthoDB" id="98443at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="PTHR11706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="418" mass="44835" checksum="A4D79DAF73C1F7C0" modified="2011-05-03" version="1">MDVKVFSRLFKSPIFLSIIIFLSVMGPGIITANVDNDAGGITTYSLAGAQFGYNLLWLFIPMIIALAVIQEMGVRMGIVSGKGLADLIREKVGIKITFLMMIALLLANFGNVLAEFSGIAVSAGIFGVPKLFALPAAALFVWLLVVKGTYKSVEKVFLLASCLYFSYIIAGFLAGPDWGMAAKSLIMPQISLSTAYITMVIGMVGTTIAPWMMFYIQSSVVEKGISLKNLKYSKLDAVFGAIIVNIVAFFIVLSCAATLYSSGVQVSNVADVSSALVPLAGQYASILFAFGFLNASLFAASILPLSTAYYICESLGFEAGVSKGFREAPVFHGLYLGLIILAVIIIMFPNVPLLSILYLSQVANGLLLPFVLIFMLLIINDKRVMGEYVNSKLFNFIAITTAVIVMGLSIGLVVTLLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-02-22" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W2</accession>
    <name>HPRT_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hypoxanthine/guanine phosphoribosyltransferase</fullName>
            <shortName evidence="1">HGPRTase</shortName>
            <ecNumber evidence="1">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hpt</name>
        <name type="ordered locus">Metbo_0197</name>
    </gene>
    <organism>
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643800.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F0T7W2"/>
    <dbReference type="SMR" id="F0T7W2"/>
    <dbReference type="STRING" id="877455.Metbo_0197"/>
    <dbReference type="EnsemblBacteria" id="ADZ08449">
        <property type="protein sequence ID" value="ADZ08449"/>
        <property type="gene ID" value="Metbo_0197"/>
    </dbReference>
    <dbReference type="GeneID" id="10276623"/>
    <dbReference type="KEGG" id="mel:Metbo_0197"/>
    <dbReference type="eggNOG" id="arCOG00030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126376_0_0_2"/>
    <dbReference type="OMA" id="FIHPISD"/>
    <dbReference type="OrthoDB" id="88302at2157"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415463" description="Hypoxanthine/guanine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <sequence length="190" mass="20685" checksum="B8CFAA58C76D23E5" modified="2011-05-03" version="1">MFEKLKISLIEAPIIKKGDYNYFVHPITDGVPLVDPDVLKEAAEGIKKFGNLNVDKIVCVEAMGIHIATALSIITGIPFVVIRKRQYGLEGEVAVHQITGYSHGELYINGLKKGDKIILVDDVVSTGGTMIAVLKAMEKMGIEIVDVFAIVEKGEGKYIVEGETGFEVKSLVKVNVVDGKVQIEGSRDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W3</accession>
    <name>F0T7W3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 6">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643801.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0198"/>
    <dbReference type="EnsemblBacteria" id="ADZ08450">
        <property type="protein sequence ID" value="ADZ08450"/>
        <property type="gene ID" value="Metbo_0198"/>
    </dbReference>
    <dbReference type="GeneID" id="10276624"/>
    <dbReference type="KEGG" id="mel:Metbo_0198"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="OMA" id="PGQVLGC"/>
    <dbReference type="OrthoDB" id="87520at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="PTHR10762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="337" mass="37256" checksum="54868084D52DFC40" modified="2011-05-03" version="1">MSGHELEIDEIIKRVKELKAGTVGLQFPEGLKLHAVEVARRIEVETGATVIISADPCFGACDVADVDMNGVADVLVHFGHKPLPLNYDMPVIFVDASSKMELLGSVGNAMSLLEGYKKIGLVTTVQHLKQLEEVAEFLEQNGKEVVINEGAGTTWGQVLGCNFSSIKNLDVEAFLYVGSGNFHALGITLFTDKPVIIADPYLDEARLITDFSDRILRIRFARIAKAMDAKTFGIIVSTKKGQNRLDTAKELKNLLNAEGREGFILLLDDVSPEKLLPYMDLDAFVMTACPRIAIDDSSRYKKPLLTPQELEIAIGKREWENYEIDEIKYGGLNNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T801</accession>
    <name>F0T801_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644978.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09627">
        <property type="protein sequence ID" value="ADZ09627"/>
        <property type="gene ID" value="Metbo_1387"/>
    </dbReference>
    <dbReference type="GeneID" id="10277838"/>
    <dbReference type="KEGG" id="mel:Metbo_1387"/>
    <dbReference type="eggNOG" id="arCOG11032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987653_0_0_2"/>
    <dbReference type="OMA" id="FHEVLDS"/>
    <dbReference type="OrthoDB" id="122520at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="13734" checksum="06C7B0FC6C6805A0" modified="2011-05-03" version="1">MATQSIEIEELEEQLILLYRFVAQINSLKRFYYSGMDYKPVFEDDTGILTELLENEESEEILKTCILELEEDKGNKDLKFHEVLDSYDVEELYNKYELQDVVEVNKLDLKKIVDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T802</accession>
    <name>F0T802_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn_dep_PLPC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644979.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09628">
        <property type="protein sequence ID" value="ADZ09628"/>
        <property type="gene ID" value="Metbo_1388"/>
    </dbReference>
    <dbReference type="GeneID" id="10277839"/>
    <dbReference type="KEGG" id="mel:Metbo_1388"/>
    <dbReference type="eggNOG" id="arCOG10205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094458_0_0_2"/>
    <dbReference type="OMA" id="VASHYIS"/>
    <dbReference type="OrthoDB" id="85485at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="SSF48537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn_dep_PLPC" evidence="1">
        <location>
            <begin position="19"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="20887" checksum="E16A17E9357687DD" modified="2011-05-03" version="1">MLVFMVQIEPAAAWAEPNHYEIVYQVYYTLPDDVHENLSLFEMMEGAGDPDLRFLDFQNHHYPQSQVKADYWLNQGRNYYLNQDYGNASYCFGVASHYISDGLCPPHTGGGHTGYLHTKFELEALCFTPHINMSENQSMDELYNNSTANWMTWLKTGDRSSIQDSLNNAASESYIQIKKSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T810</accession>
    <name>F0T810_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644987.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1396"/>
    <dbReference type="EnsemblBacteria" id="ADZ09636">
        <property type="protein sequence ID" value="ADZ09636"/>
        <property type="gene ID" value="Metbo_1396"/>
    </dbReference>
    <dbReference type="GeneID" id="24964555"/>
    <dbReference type="KEGG" id="mel:Metbo_1396"/>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259099_0_0_2"/>
    <dbReference type="OrthoDB" id="22337at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="173"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="25308" checksum="3A3D99F63BAD31A0" modified="2011-05-03" version="1">MDSSYKWIVYGGLSLLLSLFVYSILLSDQKLSITYIFFLFILGSSTWLIMTGIFLKMKYYNYMIYLILTAIILVVVTTLILNQASSPEYHGSNKYSYIIVMVFILTTIYETYGIIKEMDLYQHVIKSFEKDLITDPMDIKALNNKGAALSKRNFNQEALKCFDKILEIDSKDAVAWHNRGVMLDKLRKHQEAIKCYDKALKLDPKFENAKNTGKIILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T811</accession>
    <name>F0T811_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09637">
        <property type="protein sequence ID" value="ADZ09637"/>
        <property type="gene ID" value="Metbo_1397"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1397"/>
    <dbReference type="HOGENOM" id="CLU_2504963_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025671">
        <property type="entry name" value="HXXEE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13787">
        <property type="entry name" value="HXXEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9627" checksum="1826B7BCA93DEE03" modified="2011-05-03" version="1">MVGMGLIFFQLLINGVQHTIIFQIKKTGYNPGFLTPWLVLNPFCVITIFYAYYIHALSSLDWLLAIILGVGFIGVLFAKTMSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T887</accession>
    <name>F0T887_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein Metbo_0247</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643850.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0247"/>
    <dbReference type="EnsemblBacteria" id="ADZ08499">
        <property type="protein sequence ID" value="ADZ08499"/>
        <property type="gene ID" value="Metbo_0247"/>
    </dbReference>
    <dbReference type="GeneID" id="10276679"/>
    <dbReference type="KEGG" id="mel:Metbo_0247"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_3_0_2"/>
    <dbReference type="OMA" id="LWVNLSG"/>
    <dbReference type="OrthoDB" id="121009at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="11531" checksum="E8F14DE74C721027" modified="2011-05-03" version="1">MLILTTPNIEGHKIVEYYGLVTGESLLGANVYKDLFSGVRDVVGGRTSAYEEELKKARDLAIGSMEEKTSKKGGNAIIGIRIKYNNLGGTMGNTIMVTVTGTAVKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T897</accession>
    <name>F0T897_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinylglutamate desuccinylase/aspartoacylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0257</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643860.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0257"/>
    <dbReference type="EnsemblBacteria" id="ADZ08509">
        <property type="protein sequence ID" value="ADZ08509"/>
        <property type="gene ID" value="Metbo_0257"/>
    </dbReference>
    <dbReference type="GeneID" id="10276689"/>
    <dbReference type="KEGG" id="mel:Metbo_0257"/>
    <dbReference type="eggNOG" id="arCOG02889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159055_0_0_2"/>
    <dbReference type="OMA" id="HGNEIPP"/>
    <dbReference type="OrthoDB" id="68401at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="25625" checksum="DC5F3F12197775E9" modified="2011-05-03" version="1">MASKHLTIVTHKTGGAIDQNSVLMDHVPDGDISYKLIKAARYGTPMMKMGNGSPKVMITAGVHGNELPPQIAALELLEYLDAENLNGTVYVVPFAVPNATKNNSRRFKGFDMNRSAKKSGSATNKILMATDTLGVLSLADFHSTKPGSNPGIESVFCSKKPCSESVKIAKHITNATSSKVICHKVAGALYGGALEDECNLKYIAAVTCEVLSENNLVRAGSVEASLRQMISYLEYFDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G2</accession>
    <name>F0T8G2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09713">
        <property type="protein sequence ID" value="ADZ09713"/>
        <property type="gene ID" value="Metbo_1479"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1479"/>
    <dbReference type="HOGENOM" id="CLU_2893263_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="62" mass="7255" checksum="5EB9811E397601C7" modified="2011-05-03" version="1">MEYDLSLKRLKKDYLSLITCEKIIITNNGLIRLRYNISDEEIKDNPLDLNVIGETTINLPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J0</accession>
    <name>F0T8J0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1510"/>
    <dbReference type="EnsemblBacteria" id="ADZ09741">
        <property type="protein sequence ID" value="ADZ09741"/>
        <property type="gene ID" value="Metbo_1510"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1510"/>
    <dbReference type="eggNOG" id="arCOG01681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_2_2"/>
    <dbReference type="OMA" id="HETSYKT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="91" mass="10293" checksum="92F5C7CD6C39C8BA" modified="2011-05-03" version="1">MAKIFKALSNPNRLELYLQIVKQHETSYKTCGGCLISDITKSLNIGAPTISHHLKELSSAELIYTEKKGRYLVAKVNEDVVNQVNEYLKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J9</accession>
    <name>F0T8J9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MATE efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645101.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09750">
        <property type="protein sequence ID" value="ADZ09750"/>
        <property type="gene ID" value="Metbo_1520"/>
    </dbReference>
    <dbReference type="GeneID" id="10277971"/>
    <dbReference type="KEGG" id="mel:Metbo_1520"/>
    <dbReference type="eggNOG" id="arCOG01731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_1_2"/>
    <dbReference type="OMA" id="EFWILLK"/>
    <dbReference type="OrthoDB" id="52466at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="480" mass="51104" checksum="B65D7DD8744298BE" modified="2011-05-03" version="1">MENNEPLQSSPNLPTEDDKTEGVSTILGDPKKALIKLSGPMIIAMILMSLYNVVNAVWVAGLGGDALAAVGFVTPLFMMFMGLSNGLGAGAASAISRYIGEDNKKSVNNAAMHSVIITIGISVILSVVLLIFLKPLLLLFGAGSTLNLAVEYGQIIIGGTILSLFVGASYGILRAEGDVKRTMYAMVLSSVVNMILDPILIYWAGWGISGAAWGTIISTALVAIILLYWLFIKKDTYVSLTLKDFKPDLKVTKNILSVGLPASVEFFVMSILAIFLNILLVKVAGTDAVAVYNTGWRVVMISMSPIIGVGLSVVTIVGASYGAKKYGNISMIHNYSVKIGLVVSSIICVLIYLLAPYIAMLFTYSSTSAYLEPAIASFLQVMCLFFIFLPLGIMSSSAFQGVGKGTTSLILTVLREVVFVVLSAYILAITLGWGENGVWWGIVIGGLLGGLVAYAAARIYIKGLNHDKNTGIHEDIILAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K1</accession>
    <name>F0T8K1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645103.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1522"/>
    <dbReference type="EnsemblBacteria" id="ADZ09752">
        <property type="protein sequence ID" value="ADZ09752"/>
        <property type="gene ID" value="Metbo_1522"/>
    </dbReference>
    <dbReference type="GeneID" id="10277973"/>
    <dbReference type="KEGG" id="mel:Metbo_1522"/>
    <dbReference type="HOGENOM" id="CLU_1821032_0_0_2"/>
    <dbReference type="OrthoDB" id="119207at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="16536" checksum="7E7B89303566874D" modified="2011-05-03" version="1">MALDDKNNPGETKPKYHKKEFNGDLEPATEVNTTFSRRRAVVRIGLGLMAMVLWFLVGVSPTIIFSSSIQEFIGVFSFFVEYPVMFLLVAFLNIFLGLIVYLFIGVWWWAAFHLIWSIRWFYGYFKYRKTSLVEVIKNMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K8</accession>
    <name>F0T8K8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645110.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1529"/>
    <dbReference type="EnsemblBacteria" id="ADZ09759">
        <property type="protein sequence ID" value="ADZ09759"/>
        <property type="gene ID" value="Metbo_1529"/>
    </dbReference>
    <dbReference type="GeneID" id="10277984"/>
    <dbReference type="KEGG" id="mel:Metbo_1529"/>
    <dbReference type="eggNOG" id="arCOG01624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_3_2_2"/>
    <dbReference type="OMA" id="WEMAQIQ"/>
    <dbReference type="OrthoDB" id="43721at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="44916" checksum="A55B9484FD8ED1D9" modified="2011-05-03" version="1">MIYRDFGKTNEKVSVLGFGCMRLPVVGGDPSNIDEEKAIKMVRYSIDQGVNVIDTAYPYHGFGLDQAGASEPFVAKVLRDGYREKVKLATKLPIWMLKTREDMDKYLNEQLKRLETDHIDFYMVHGINRNYWENIKELGFDEFLDDAISDGRIKYAGFSFHHRIELFKEVVDHYDWSFCLIQYNYLDENYQAGKEGLEYAYNKNMGIAVMEPLRGGQLAHNMPQKVQELFDNYEVKRSPAEWALRWVWNHPEVSVVLSGMNTMEELEENLKIAEEANPNSLGDEELNTLKQAKSIFENKLQINCTSCGYCLPCPSGVNIPENFQKYNDYYLFGSPEEKAGYQFSYDALVLENERASQCIECGICEEHCTQDIKIIKELKKVKELYESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L7</accession>
    <name>F0T8L7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="8">DHOD</shortName>
            <shortName evidence="8">DHODase</shortName>
            <shortName evidence="8">DHOdehase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pyrD</name>
        <name evidence="10" type="ordered locus">Metbo_0307</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-dihydroorotate + A = AH2 + orotate</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="8">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0307"/>
    <dbReference type="EnsemblBacteria" id="ADZ08559">
        <property type="protein sequence ID" value="ADZ08559"/>
        <property type="gene ID" value="Metbo_0307"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0307"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_4_3_2"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 8" id="KW-0288">FMN</keyword>
    <keyword evidence="7 8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="9">
        <location>
            <begin position="3"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="260"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="31289" checksum="995B64CDE1C73212" modified="2011-05-03" version="1">MEIELCGIKMKNPTMLAAGILGSTASSLNWAAKSGAGAVVTKSFGLNSNKGYSNPTTVEVTGGVINAIGLSNPGVENFQMELKKLDGSVPAIASIYGANPDEFSKIATHIQEDVDAIELNVSCPHAMGGCGAAIGQDPLLTSSIVSAVKESVNVPILVKLTPNVTDIVEVAVSAQDAGADALTLINSLGPGMKIDITTGKPILSNRFGGMSGPAIKPVAVRCVYDVFDAVEIPVVGVGGIRNYEDVLEFIYAGATCVQIGTSIMYEGMEIFGKVVNDLEVFMEKMGYTKLEEMVGISHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N3</accession>
    <name>F0T8N3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ordered locus">Metbo_0323</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643926.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0323"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="EnsemblBacteria" id="ADZ08575">
        <property type="protein sequence ID" value="ADZ08575"/>
        <property type="gene ID" value="Metbo_0323"/>
    </dbReference>
    <dbReference type="GeneID" id="10276760"/>
    <dbReference type="KEGG" id="mel:Metbo_0323"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OMA" id="VDKTGPH"/>
    <dbReference type="OrthoDB" id="89767at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005049684" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023549729" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="10"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="210" mass="22627" checksum="F5D447632C1D5D5D" modified="2011-05-03" version="1">MNDKDRLKGTTTVGVTCKDGIVFATERRASMGNLIAHKVADKIFKIDDHIGATIAGSVSDAQKLMGYISAEVALYRLRNGAPMSVEAAANMTSNILHASRFYPYYVQTLLGGVDEKGPALFSLDPSGGVIKDLMISTGSGSPVAYGVLEDRYNQEMDIEEGTSVVIRAIKAAMERDTYSGNGIKVATITKEEGFKILPDDEVAKKIEELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q4</accession>
    <name>F0T8Q4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0344"/>
    <dbReference type="EnsemblBacteria" id="ADZ08596">
        <property type="protein sequence ID" value="ADZ08596"/>
        <property type="gene ID" value="Metbo_0344"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0344"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="FMFREES"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="66" mass="7385" checksum="2701D42D267971BC" modified="2011-05-03" version="1">MDQSPKTAPIQEGEEYDVKIEDVGKEGDGITRVEGFVVFVPDTKVGDEVKVKITSVRRRFAFAEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U8</accession>
    <name>F0T8U8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09776">
        <property type="protein sequence ID" value="ADZ09776"/>
        <property type="gene ID" value="Metbo_1547"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1547"/>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942019_0_0_2"/>
    <dbReference type="OrthoDB" id="25869at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="33170" checksum="6C298FABB67C40FC" modified="2011-05-03" version="1" precursor="true">MLLLSGLVLFFNVSGASAVTTNNINVTNHTKTTVASQATVKTSFTPSQIGAASSKVKSYYEKNNKLPCYVTINNKNVTMPQFLQLISDDIYQLSNKKTTSITLKNVTAPSNPVENLKSGQLTKTEYINLAKITRSYIYANGKAPNYENCSLGKIKYQNLIYSFSKILAFQAANNRLPNYDSVAPFSKTSTTNKISSNALASVDSIGYAEAKFRDVQGQSSASVMDKCGYGDCWADSSWLYNKLSAAGIPVRIMSTTSGGIYYLHRWVEINTGNGWQTWNYAKYNSQHYSALGSGHYVVKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W7</accession>
    <name>F0T8W7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645146.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09795">
        <property type="protein sequence ID" value="ADZ09795"/>
        <property type="gene ID" value="Metbo_1568"/>
    </dbReference>
    <dbReference type="GeneID" id="10278024"/>
    <dbReference type="KEGG" id="mel:Metbo_1568"/>
    <dbReference type="HOGENOM" id="CLU_1682709_0_0_2"/>
    <dbReference type="OrthoDB" id="139955at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="156" mass="18134" checksum="FD357589CB646462" modified="2011-05-03" version="1">MGAAIKSPEHGCEPLSYIACSIPCKANQIYIKKIKEELNPDELQTFNSTTKFMQQLFQSYTAEHLILLNDENFSSVINVKRPSILAHEAYHIVEYETFDQTHTGEEIDEYAQKLVELYYLSLKDDEIEGEYDKICSTKKGNRKYGRLRIITNFHMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z3</accession>
    <name>F0T8Z3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645172.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1594"/>
    <dbReference type="EnsemblBacteria" id="ADZ09821">
        <property type="protein sequence ID" value="ADZ09821"/>
        <property type="gene ID" value="Metbo_1594"/>
    </dbReference>
    <dbReference type="GeneID" id="10278051"/>
    <dbReference type="KEGG" id="mel:Metbo_1594"/>
    <dbReference type="eggNOG" id="arCOG00213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="40323at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PBP_domain"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="26"/>
        </location>
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        <location>
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            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="30627" checksum="633D693656DAEEF3" modified="2011-05-03" version="1" precursor="true">MKLKNNYVIITVVAVLVIIIAAYAGVTQYSQSNDITIEGSTSVYPVATALAKAYMEKHPNVQIDVQGGDSEAGINNVKAGKVDIGMSSRNLSSTESQDLSQYRIGKDPVAVIVNPVNPVNTITTNELKDIYTGKTTNWEALGGNNIQITPVIREKGSGTRYDFEQYIMDGDEYAKNIVVTTSTYGALQTVAVSPDTIGYVSNNALSSDVKTLKINNVTLNQENVENGSYNLTRSMLFLVKGSATGEIKDFIDFSLSTEGQNIINSVEYGSQSNNTNTGIGIGPVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z4</accession>
    <name>F0T8Z4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1595"/>
    <dbReference type="EnsemblBacteria" id="ADZ09822">
        <property type="protein sequence ID" value="ADZ09822"/>
        <property type="gene ID" value="Metbo_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1595"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="PMWFFLN"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
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    </dbReference>
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        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
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            <end position="79"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="257"/>
        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="25"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="527" mass="58402" checksum="9CA058FB6C9C11D9" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T901</accession>
    <name>F0T901_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09829">
        <property type="protein sequence ID" value="ADZ09829"/>
        <property type="gene ID" value="Metbo_1602"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1602"/>
    <dbReference type="HOGENOM" id="CLU_3163121_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="47" mass="5435" checksum="A0F503E26A7AA7DB" modified="2011-05-03" version="1">MTETKIEFKCLKCGTHYDPNINLECPVCGKQQKLSTHHNSKHIIKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T904</accession>
    <name>F0T904_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HIGH_NTase1_ass domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643977.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0374"/>
    <dbReference type="EnsemblBacteria" id="ADZ08626">
        <property type="protein sequence ID" value="ADZ08626"/>
        <property type="gene ID" value="Metbo_0374"/>
    </dbReference>
    <dbReference type="GeneID" id="10276811"/>
    <dbReference type="KEGG" id="mel:Metbo_0374"/>
    <dbReference type="eggNOG" id="arCOG03224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777585_0_0_2"/>
    <dbReference type="OMA" id="MGSGQYM"/>
    <dbReference type="OrthoDB" id="41648at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032266">
        <property type="entry name" value="HIGH_NTase1_ass"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16581">
        <property type="entry name" value="HIGH_NTase1_ass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIGH_NTase1_ass" evidence="1">
        <location>
            <begin position="138"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="365" mass="42016" checksum="EFD2A616F18B8C02" modified="2011-05-03" version="1">MVEAELITKIFKRDEKLFKGDLNKQPSYGDVKSDVKIIADFTEYSPMHLGHRYCMEQAKKQVPEGIFVAIVPGLFERNGRGNPYIMTREARADTAVSVGADIVVEGPPMGIMGSGQYSMCLAATFKALDADYIPRGYRPFDGFQTILNRISNGFGVAPKPYKIVDMETKDVLYQGKLDEDNYVIVSLSKSLNKINFDFKNKFIFIKRLEGVSGTKIRENVGDGDLHAVETMLPTETMRILEREIEYGRAPMDQLRNYNGILDRVNNSNINELRSLTMMDERTLMSLIENRPFKTVLELEKSISRGFSRHHKNRVLSALEAGIFKEDIHKYIDRYPSVIRVLKYKNEEVLNEFRKRIPHRRLELWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T923</accession>
    <name>F0T923_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-vinyl reductase 4VR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643996.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0393"/>
    <dbReference type="EnsemblBacteria" id="ADZ08645">
        <property type="protein sequence ID" value="ADZ08645"/>
        <property type="gene ID" value="Metbo_0393"/>
    </dbReference>
    <dbReference type="GeneID" id="10276830"/>
    <dbReference type="KEGG" id="mel:Metbo_0393"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387613_0_0_2"/>
    <dbReference type="OMA" id="CWTNGYS"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="136"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="199" mass="22469" checksum="F33FCD1B3F252363" modified="2011-05-03" version="1">MENGKIKRAIKNYRELAAHEIEVEKCVGDALNGPRPEIDGKLELSQMINPKRPFFASMVNSDEKYVSTFRMGHFNTPEILSKSVRGANYVGGMEFGSNLIKQKLVTDFDDLVSFLLDYKFGILDIVDENEINGKTVLDLRVYECIECACLPNLGKPTCIFEAGMITGILTEMTKKDVIAKEVRCWTNGYSFCQFEVTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T964</accession>
    <name>F0T964_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">V-ATPase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">atpE</name>
        <name evidence="6" type="ordered locus">Metbo_0434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644037.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0434"/>
    <dbReference type="EnsemblBacteria" id="ADZ08686">
        <property type="protein sequence ID" value="ADZ08686"/>
        <property type="gene ID" value="Metbo_0434"/>
    </dbReference>
    <dbReference type="GeneID" id="10276871"/>
    <dbReference type="KEGG" id="mel:Metbo_0434"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105846_1_0_2"/>
    <dbReference type="OMA" id="YAGNIDC"/>
    <dbReference type="OrthoDB" id="93762at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="PTHR45715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="22937" checksum="0B4CD7A2E92B744E" modified="2011-05-03" version="1">MSAGTEKIVSSIISEAQTKASSIEEEAEVESKSILEEGEKIALIEKEKILEDGKKQSTMRYQQIISEAKMNSRRMELDAREEIIEESFKKAVENLKEIASSDSTEYKESLNEIIIEAATEIGGGDLIVSVKAEDVAKIKVSISSIENEVKEKTGNETKLEIGDNINTIGGAVLKTKNGEIEVNNTIEARLLRFKKSLRSEVARILFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A2</accession>
    <name>F0T9A2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein Metbo_1626</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09853">
        <property type="protein sequence ID" value="ADZ09853"/>
        <property type="gene ID" value="Metbo_1626"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1626"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_3_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="12283" checksum="137C21462B6CDC7D" modified="2011-05-03" version="1">MNIRIFTTSTVLGENVTKHMGLVTGESLIGANLYKDMLGEVKGVVKKKQSQYEVQLGEARKIAIDSMMEKAEKLGANAIIGTRVEYNNLGGSMGNTIMVTVTGTAVQVTATENSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B5</accession>
    <name>F0T9B5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645217.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1640"/>
    <dbReference type="EnsemblBacteria" id="ADZ09866">
        <property type="protein sequence ID" value="ADZ09866"/>
        <property type="gene ID" value="Metbo_1640"/>
    </dbReference>
    <dbReference type="GeneID" id="10278097"/>
    <dbReference type="KEGG" id="mel:Metbo_1640"/>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313006_0_0_2"/>
    <dbReference type="OrthoDB" id="7264at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1016"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="529"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="644"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="759"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="3">
        <location>
            <begin position="874"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1040" mass="106051" checksum="8D8690785E3CF8E6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B7</accession>
    <name>F0T9B7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_1642</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645219.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1642"/>
    <dbReference type="EnsemblBacteria" id="ADZ09868">
        <property type="protein sequence ID" value="ADZ09868"/>
        <property type="gene ID" value="Metbo_1642"/>
    </dbReference>
    <dbReference type="GeneID" id="10278099"/>
    <dbReference type="KEGG" id="mel:Metbo_1642"/>
    <dbReference type="eggNOG" id="arCOG00798">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_6_0_2"/>
    <dbReference type="OMA" id="HCANILI"/>
    <dbReference type="OrthoDB" id="31899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="9"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="12">
        <location>
            <begin position="295"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="933" mass="109375" checksum="D3345B97E0884BF2" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C0</accession>
    <name>F0T9C0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645222.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09871">
        <property type="protein sequence ID" value="ADZ09871"/>
        <property type="gene ID" value="Metbo_1645"/>
    </dbReference>
    <dbReference type="GeneID" id="10278102"/>
    <dbReference type="KEGG" id="mel:Metbo_1645"/>
    <dbReference type="eggNOG" id="arCOG11273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103517_0_0_2"/>
    <dbReference type="OrthoDB" id="142095at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="12708" checksum="FED83A83AB39698E" modified="2011-05-03" version="1">MLKRYSTLSAIIIGIFFVGFLTFILPANIPGSDILVILIFIVGGFTSTYLSKTNKAIIGFYEGLTGSIVGYLPMILIFRSVTSVLIILMIINPILGFLGGFIAKYWRTRLNNKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E6</accession>
    <name>F0T9E6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1671"/>
    <dbReference type="EnsemblBacteria" id="ADZ09897">
        <property type="protein sequence ID" value="ADZ09897"/>
        <property type="gene ID" value="Metbo_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1671"/>
    <dbReference type="HOGENOM" id="CLU_3003156_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="56" mass="6180" checksum="3176BFC4BA066682" modified="2011-05-03" version="1" precursor="true">MVNWNMIMVGIVLVAVGTFFSLFGTVGSIIGFIMAISVVIWFIKETRANKPARMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H4</accession>
    <name>F0T9H4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644073.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0470"/>
    <dbReference type="EnsemblBacteria" id="ADZ08722">
        <property type="protein sequence ID" value="ADZ08722"/>
        <property type="gene ID" value="Metbo_0470"/>
    </dbReference>
    <dbReference type="GeneID" id="10276908"/>
    <dbReference type="KEGG" id="mel:Metbo_0470"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609189_0_0_2"/>
    <dbReference type="OMA" id="ESYKYIR"/>
    <dbReference type="OrthoDB" id="20865at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="456" mass="51240" checksum="90A1D62C3D835E7D" modified="2011-05-03" version="1">MDSIEQEFNPDIELMEESKDVDGLIRALKNEDYLIRKDAAISLKRLGDERTVGALIESLKYESWQDEFTVLIAVRENSAEALGIIGDKIAVPALIETLLVDSDEEVRWKAAAALGRLKDDRGVDALITALNDVSWAVRRNATIALGDIGNEKGYEPLLNSLTDSDWHVRKYAAIALGKIGDERAIKPLVNTLKDTDSDVRWKSVMALGKIGKPAVGELLKSFDTDDWQLRSQIAVVLGKIKDERAVEPLIDSLYNSRYKNQNKYVRGRIIEALGNIGDERAVDALINALDDQYIYVRIKAEEALKKIQALGKGSWIVNFENGEISFEYPNKWEIIETTDDKKVIIGGVANNSIHFSINRNEEAEDISAEEFAEILKNVFMMQNSSITSEMGFISRGMETYILVGENPHGSSVTKIMIVAYKKKDMLYYLWFAGEPDNFEIFNDDVDLIVDSFKILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I3</accession>
    <name>F0T9I3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0479</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644082.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0479"/>
    <dbReference type="EnsemblBacteria" id="ADZ08731">
        <property type="protein sequence ID" value="ADZ08731"/>
        <property type="gene ID" value="Metbo_0479"/>
    </dbReference>
    <dbReference type="GeneID" id="10276919"/>
    <dbReference type="KEGG" id="mel:Metbo_0479"/>
    <dbReference type="eggNOG" id="arCOG00168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_2_2"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="86965at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="66"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="282" mass="29977" checksum="6683C9F35A7EA59A" modified="2011-05-03" version="1">MNRILSPKISQKIMKGVFWGSGILTILLLVMIIGYVLINGLPVINFEFLFSDPIDSGRAGGIFPMIVSTIYVTFVALVVAIPLGIGGAVYLAEYAGESNIVKAIRFGSETLASIPSIVFGLFGYAFFVIYLGLGFCVLSGGLTLALMALPTILRTAEVSIESVPRSYTEGSLALGANKWQTIYEVVLPAAIPGIITGIILGMARAIEETAAILYTVGASLAVPVSIFDPARPLPLHLYTLATEGISMPNAYGTAAVLVILILVITIVTNALVDRYIKKMMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J8</accession>
    <name>F0T9J8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="EnsemblBacteria" id="ADZ08746">
        <property type="protein sequence ID" value="ADZ08746"/>
        <property type="gene ID" value="Metbo_0494"/>
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    <dbReference type="eggNOG" id="arCOG00609">
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    </dbReference>
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    <sequence length="337" mass="37407" checksum="E3D1889BDA348D6A" modified="2011-05-03" version="1">MRDYELIETYISKYFDLGGFHKTPEGSFFVVHDYDYNKFEALIKDLDELGYVPFIEKYADNYRIGIADKKEVGESKSYINILLFVVTLATTIYAGYQFAGGSIWDGVAFAGALLGILGVHESAHYFAAKKHGVKSTLPYFIPAPTLIGTFGAVINVKSPIPNKNALFDLGYSGPLAGILVTIPVLIVGITLSKVVPITQGSTVFYPSPLMSIFMYFLLPPIPAGYELQIHPLLFAAWVGIIVTLLNMMPVAFLDGGHMVRSIFNENIHRIISMVGILITIVLGWYTMAVLMMLILYVNRRHPGALDDVDDLTFRRKVLAVVMLVVFILCLTHIPTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="295" mass="33382" checksum="88252AA9F5AE3558" modified="2011-05-03" version="1">MVNKITVKTILNNSKKRDDWFLGRYTLNTYAGCSMACIYCYTRGSKYGGDHGSVVSAKLNAVPVLKKQLKNCIRRNERDIILFGSAADPYSTVEKDLNLTREVLGIIKRYKFPTHILTKSKQIIRDLDIIKDIRDVAMVPDDLVDKLKNGVIVSFSFSTLDEELAEIIEPGAPSPLERLETMKEFSDAGFKTGIINMPILPFLSDSDEAIKSMVKTAKFYGAHYVLYSGLTLYGSGNSDCKTLYLDFLREHNPDLVKEYEILFQKSFAPPKKYSFELSKRFSEISSKYGVKNSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P6</accession>
    <name>F0T9P6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Drug resistance transporter, EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1700</name>
    </gene>
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    <reference evidence="4" key="1">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Land M."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09925">
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        <property type="gene ID" value="Metbo_1700"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="GNICYGA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    </feature>
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        </location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="19"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="49089" checksum="517D0A155944A702" modified="2011-05-03" version="1" precursor="true">MTNEKSCKPEHSINNRVIALLVATLASFFTPFMASAVNIAIPSIGSSFGADVILLSWIPTAYLLAAAVFAVPFGRIADIHGMKKIFTYGIIIFTVASFLCAIAPSIISLIVFRIMQGVGSAMIFVTGLAIISSVYPRNERGKAIGINVASVYIGLSMGPVLGGLLTQYFGWRSIFLVVIPLGLLVIALTLTKLKGEWAECAGESFDIPGSVIYSVALVVLIYGFSILPGLYGILMVVAGVVGLLIFAAFELRVKNPVFEVRLFKNRTFGFSSLAALINYSSTFAVIFLLSLYLQYIKGMDPQAAGIILVAQPVVMAITSPIAGRLSDKFSPGSIATIGMAITTLSLICFTFLSSSTSIEYIVVVLLVLGFGLGLFSSPNTNAIMGSVEKKFYGIASATVGTMRLIGQMLSMGIAMLVFSIFIGNVVIEPSNYPALISSVNTVFMICAGLCFVGIFASYIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S0</accession>
    <name>F0T9S0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645300.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1725"/>
    <dbReference type="EnsemblBacteria" id="ADZ09949">
        <property type="protein sequence ID" value="ADZ09949"/>
        <property type="gene ID" value="Metbo_1725"/>
    </dbReference>
    <dbReference type="GeneID" id="10278182"/>
    <dbReference type="KEGG" id="mel:Metbo_1725"/>
    <dbReference type="eggNOG" id="arCOG11273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901945_0_0_2"/>
    <dbReference type="OMA" id="WYSPSAI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="14874" checksum="1DBE7426D107804B" modified="2011-05-03" version="1">MKDLYSILIGTVMVSGLFFLVDTLFYSNNSLTTNLFIIILILGGYLVTYTSNTLKTRTAIFSGFFVGIVLIVYQIIFNSSAFDPLAMIGYLVLPGFFMMIGGFSAKITQKQMEELVDHLLKRKIESESKNTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S5</accession>
    <name>F0T9S5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645305.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1730"/>
    <dbReference type="EnsemblBacteria" id="ADZ09954">
        <property type="protein sequence ID" value="ADZ09954"/>
        <property type="gene ID" value="Metbo_1730"/>
    </dbReference>
    <dbReference type="GeneID" id="10278187"/>
    <dbReference type="KEGG" id="mel:Metbo_1730"/>
    <dbReference type="eggNOG" id="arCOG01624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_3_2_2"/>
    <dbReference type="OMA" id="YYYFFQR"/>
    <dbReference type="OrthoDB" id="35652at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="407" mass="46742" checksum="0C80665B7E63AA53" modified="2011-05-03" version="1">MQYRKIEKNGDQLSVLGYGAMRLPTKNGRTDEKRAIEQIRYAIDHGVNYLDTAIMYMNEPLVGRALRDGYREKVKLATKLPPWTVNKEEDLRSTFKVQLENFKISYIDYYMLHGLNRANWNKMLEFNALEFLDEIKKQEKILNAGFSFHGDIDTFKEIIDAYDWDFCMIQYNFLDEKNQAGTEGLEYAASKDIPVIVMEPFRGGNLARNVPEEIQAIWDEAEVKRSPAEWSLRWVLNHPEVTCVLSGMNEEEHIKENIRIADEALPNSLTEDELKLIERVADKYRELMETGCTGCRYCMPCPKGVDIAKCFEVYDSAKLFNTGNIQSKVMYAAGLGVMNDGKPGFASKCTKCGKCEKHCPQELPIMDLLDDVSNEMEGPLTKIIPPIFKAFLSIQSYRNNRKAKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X4</accession>
    <name>F0T9X4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 6</fullName>
            <shortName evidence="3">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif6</name>
        <name evidence="4" type="ordered locus">Metbo_0545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644148.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0545"/>
    <dbReference type="EnsemblBacteria" id="ADZ08797">
        <property type="protein sequence ID" value="ADZ08797"/>
        <property type="gene ID" value="Metbo_0545"/>
    </dbReference>
    <dbReference type="GeneID" id="10276990"/>
    <dbReference type="KEGG" id="mel:Metbo_0545"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OMA" id="GEDTTGP"/>
    <dbReference type="OrthoDB" id="70525at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="PTHR10784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="23694" checksum="3ABE5C137B608843" modified="2011-05-03" version="1">MIRRINMDGSPNLGVSISVSDKIAIAPPNMLDSMEDLINEALGVEVIRTPICGSNLTGALTCGNSKGFIVSKYAFDKELEVFKEKGVEVARIPDKLTAVGNIVLANDSGALVHPLISDKAIELISDVLDVEVKRGSIANYKITGSVANATNKGVLVHPATLEDELKDLEEFFKVPVDVGTVNNGTQFVGSCSIANSNGVMVGLGTTGPELARIEEAFGFLEGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA20</accession>
    <name>F0TA20_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0592</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
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        <property type="protein sequence ID" value="ADZ10023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645374.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1801"/>
    <dbReference type="EnsemblBacteria" id="ADZ10023">
        <property type="protein sequence ID" value="ADZ10023"/>
        <property type="gene ID" value="Metbo_1801"/>
    </dbReference>
    <dbReference type="GeneID" id="10278258"/>
    <dbReference type="KEGG" id="mel:Metbo_1801"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="FVYQSHR"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="24530" checksum="4F292C8FCBBAAD84" modified="2011-05-03" version="1">MNDNTIIKIKNLKKEYEDGSIIALNNVDLEIERGESVSIIGPSGSGKSTLLNMIGGLDKANEGQISVAGVNLMDNSDLSNFRSNEIGFVFQLHNLIPNLTVLENVQIPLLGTDIDDKKMEERALKLLKSVNLENKVDQKPTKLSGGERQRVAIARALVNHPSIILADEPTGALDSETQEVILKLLLDIHKKENVTLIMVTHEPYVAKQADRSIYVLDGKIKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB1</accession>
    <name>F0TAB1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644214.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0612"/>
    <dbReference type="EnsemblBacteria" id="ADZ08863">
        <property type="protein sequence ID" value="ADZ08863"/>
        <property type="gene ID" value="Metbo_0612"/>
    </dbReference>
    <dbReference type="GeneID" id="10277057"/>
    <dbReference type="KEGG" id="mel:Metbo_0612"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="OMA" id="VEWANHA"/>
    <dbReference type="OrthoDB" id="127272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TAF" evidence="6">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="67" mass="7121" checksum="1132FF4ABDD885F5" modified="2011-05-03" version="1">MELPIAPIGRIIKNAGAERVSDDAREALAKALEEKGETIASEAVKLAKHAGRKTVKASDVDLAVKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC7</accession>
    <name>F0TAC7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="3">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="3">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sucD</name>
        <name evidence="8" type="ordered locus">Metbo_0628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0628"/>
    <dbReference type="EnsemblBacteria" id="ADZ08879">
        <property type="protein sequence ID" value="ADZ08879"/>
        <property type="gene ID" value="Metbo_0628"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0628"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="7">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="3 4">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="30151" checksum="F9F88302AA1CD433" modified="2011-05-03" version="1">MIILDENTRCIVQGITGKQGSFHAEQMLKYNTNIVAGTSPGKGGQNFNGLPIYNSIEEAKDELDINASIIFVPASFAKDAAFEAISQLDLAVIITEHIPVHDSMEIVEYAKKENTIVIGPNTPGIISPGIGKLGIMPTHIFNEGNIGIVSRSGTLTYEVANQVTNAGMGQSTCVGIGGDPVVGLDFADVLQRFEDDDDTAAMVMIGEIGGNAEEMAADYIKENISKPVIAYIAGVTAPPGKRMGHAGAIIEGDAGTAETKINALEKSGAHVADRPSEIADLIWDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC8</accession>
    <name>F0TAC8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644231.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0629"/>
    <dbReference type="EnsemblBacteria" id="ADZ08880">
        <property type="protein sequence ID" value="ADZ08880"/>
        <property type="gene ID" value="Metbo_0629"/>
    </dbReference>
    <dbReference type="GeneID" id="10277074"/>
    <dbReference type="KEGG" id="mel:Metbo_0629"/>
    <dbReference type="eggNOG" id="arCOG04839">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149814_0_0_2"/>
    <dbReference type="OMA" id="AFDTYGG"/>
    <dbReference type="OrthoDB" id="88910at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012021">
        <property type="entry name" value="UCP005278"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005278">
        <property type="entry name" value="UCP005278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="240" mass="25009" checksum="54C5C14551F9D319" modified="2011-05-03" version="1">MKLTSIGADVSCNDVSCSKNMITNIEKDIHEIVDAGAFKAALTNVTGDDVVISAFVDDNMLETVNKAIVDVLSKNAEDIGDLAGISDTPQGAGEGISYAEAKIRQDRYPDGVILAFDTYGGEPFVKNVVKSSMDAARSMDHVTDIGGGNHGTIEIPGVGFVSDETDDPVVVATIEDIEAVGVVAGAMVGAALGNKNVYLVKRGTPSYVIPGSVIVSVSAFMNGNVMDLSVPLAQRMRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE6</accession>
    <name>F0TAE6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0647"/>
    <dbReference type="EnsemblBacteria" id="ADZ08898">
        <property type="protein sequence ID" value="ADZ08898"/>
        <property type="gene ID" value="Metbo_0647"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0647"/>
    <dbReference type="eggNOG" id="arCOG06503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119854_0_0_2"/>
    <dbReference type="OMA" id="QLNDYPY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="175" mass="20535" checksum="1D4EF92766B9D0B2" modified="2011-05-03" version="1">MDFNEDEFNDEEVNETTDEVNEETEEVKDSEETLGDEESTEDVEDSEVILGDEVVTEEEGEDLPFAKAEVVRLMKKNLDKDKMIRERVKVEMNKFLGEVLERVCEQLNDYPYTTIEYEMLKESIYPYTNIKRINEEKFRILKHLDAIKADCDALSLDVKKSLKLKDVDDDDDEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM9</accession>
    <name>F0TAM9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1880"/>
    <dbReference type="EnsemblBacteria" id="ADZ10101">
        <property type="protein sequence ID" value="ADZ10101"/>
        <property type="gene ID" value="Metbo_1880"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1880"/>
    <dbReference type="eggNOG" id="arCOG06501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101012_2_0_2"/>
    <dbReference type="OMA" id="SADHEGK"/>
    <dbReference type="OrthoDB" id="85057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="20216" checksum="276B724FD0105362" modified="2011-05-03" version="1">MEFEDLLKQLEDDDPAVRRDAVEQLGVEGVKVNVEPLMKALNDENPDVRFQASKSLAEVGEPAVDPLIQALKGDEGNTKRYATFALKNMGDDNVVVHLIDALEDDDWSVRKTSAKSLGEMGNQKAVEPLINTLQDDDWGVRCSAAKALGDLGDETAIDPLKKARRAAKGDKEFKKVATKAIKKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN5</accession>
    <name>F0TAN5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1887"/>
    <dbReference type="EnsemblBacteria" id="ADZ10107">
        <property type="protein sequence ID" value="ADZ10107"/>
        <property type="gene ID" value="Metbo_1887"/>
    </dbReference>
    <dbReference type="GeneID" id="10278345"/>
    <dbReference type="KEGG" id="mel:Metbo_1887"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064007_0_0_2"/>
    <dbReference type="OMA" id="LGNHEWS"/>
    <dbReference type="OrthoDB" id="78884at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="18"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="30310" checksum="1EBF0F6BEA9E0259" modified="2011-05-03" version="1">MGFYRGKLIELPKEGHAIVVTDIHGNLTDYNKYLELWKKYGEKNFHFIITGDFIHAMGKKDDRSIDILESVMHNFKHCKNFHALLGNHEWSAISMVSVYKGGVNQSHSFIKLLKDRFGNGWEVKLEEYQNFFKKLPVAVKTDNKLFISHGGPPRRINSIEDIVHITDKGYGEDNNKLYQLLWNREENFNKEDLNNFLRVVNCNAMIVGHTPVDGVKMVYDKQLVVSSSFSLGKKSYLVINLKNNIERARDLLSMENLHWYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN8</accession>
    <name>F0TAN8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease NucS</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nucS</name>
        <name evidence="3" type="ordered locus">Metbo_1890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645461.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1890"/>
    <dbReference type="EnsemblBacteria" id="ADZ10110">
        <property type="protein sequence ID" value="ADZ10110"/>
        <property type="gene ID" value="Metbo_1890"/>
    </dbReference>
    <dbReference type="GeneID" id="10278348"/>
    <dbReference type="KEGG" id="mel:Metbo_1890"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="OMA" id="RCSVDYA"/>
    <dbReference type="OrthoDB" id="96363at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="PTHR38814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="29011" checksum="EBD9BA66CD37F8C5" modified="2011-05-03" version="1">MKFLSEENPDHQRTFEIINEGLSKKAVVVVVACCTVNYDGRARSKLGAGDRMVMIKSDGSFMVHQNINLEPVNWQPPKSNCSAVLKDGNIILEGSRRSPPERLQVVIHKTYVASYYNGKDNKDLELTGYEENMREMIFKTPDIIEEGFRPSTKEYPTSTGFIDVLGKDKNGNVVVLELKSRRAGINAVKQLKRYLDDFSDHKEFVRGILVAPSVTDDAAELLEGFKLEFKPLDPPREFGADKNLTLDFFSKTQDTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS9</accession>
    <name>F0TAS9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644306.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0704"/>
    <dbReference type="EnsemblBacteria" id="ADZ08955">
        <property type="protein sequence ID" value="ADZ08955"/>
        <property type="gene ID" value="Metbo_0704"/>
    </dbReference>
    <dbReference type="GeneID" id="10277149"/>
    <dbReference type="KEGG" id="mel:Metbo_0704"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="OMA" id="AVPGNCD"/>
    <dbReference type="OrthoDB" id="49505at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="24070" checksum="C3484DB8E0BC3FA2" modified="2011-05-03" version="1">MKIVAFSDIHGKYGKVIEFLKNNQVDLVILTGDITHFGPPELAGEILNEIASFDVPVLAIPGNCDPAELYGNMDNSNAINIHGRSVTINNVGICGFGGSNPTPFKTPLEFDEVEIYENAKKALEEVKDSKVTLFVTHAPPVDTKVDVLPSGDHVGSISIRKIIEESQPTINVCGHIHESIATDKIGETEVINPGMTAGGYACMINIDDSNEDDIKVESKIIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB14</accession>
    <name>F0TB14_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645511.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10160">
        <property type="protein sequence ID" value="ADZ10160"/>
        <property type="gene ID" value="Metbo_1940"/>
    </dbReference>
    <dbReference type="GeneID" id="10278400"/>
    <dbReference type="KEGG" id="mel:Metbo_1940"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149827_0_0_2"/>
    <dbReference type="OMA" id="CGKYYEL"/>
    <dbReference type="OrthoDB" id="126931at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="21632" checksum="EFA27579E3D8123E" modified="2011-05-03" version="1">MSYLVCEQCGKYYELDEGKTSFSYEKCECGGKLSYRNNLDRTATAANPVITNNKCGKCGAEIPPNTVICNNCGNNSEQLGIEQSTGLSIAGVAVGFGFLLLTTLIAVFALFGNNIPLKAEDIPYRMLIIFGVVATIVSILSGLVASYIGGSTKFQDGFLNGGLVGVVLGILVALTSGSLASIEVLLIFGFLSGMGGLVGTVLKRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB55</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_1981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Euryarchaeota</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1981"/>
    <dbReference type="EnsemblBacteria" id="ADZ10201">
        <property type="protein sequence ID" value="ADZ10201"/>
        <property type="gene ID" value="Metbo_1981"/>
    </dbReference>
    <dbReference type="GeneID" id="10278441"/>
    <dbReference type="KEGG" id="mel:Metbo_1981"/>
    <dbReference type="eggNOG" id="arCOG14005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413691_0_0_2"/>
    <dbReference type="OrthoDB" id="36549at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="386"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="656" mass="74146" checksum="EA4DDA6AC8774086" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB57</accession>
    <name>F0TB57_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0757</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0757"/>
    <dbReference type="EnsemblBacteria" id="ADZ09008">
        <property type="protein sequence ID" value="ADZ09008"/>
        <property type="gene ID" value="Metbo_0757"/>
    </dbReference>
    <dbReference type="GeneID" id="25394715"/>
    <dbReference type="KEGG" id="mel:Metbo_0757"/>
    <dbReference type="eggNOG" id="arCOG02348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="NTEYARI"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
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        <property type="entry name" value="HATPase_C"/>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
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    </dbReference>
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        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
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    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="971" mass="112028" checksum="41BE107E77FA2522" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB80</accession>
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        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
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        <name evidence="10" type="ordered locus">Metbo_0780</name>
    </gene>
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        <lineage>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644382.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0780"/>
    <dbReference type="EnsemblBacteria" id="ADZ09031">
        <property type="protein sequence ID" value="ADZ09031"/>
        <property type="gene ID" value="Metbo_0780"/>
    </dbReference>
    <dbReference type="GeneID" id="10277228"/>
    <dbReference type="KEGG" id="mel:Metbo_0780"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_0_2"/>
    <dbReference type="OMA" id="YIKKCGE"/>
    <dbReference type="OrthoDB" id="60366at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_S3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_S3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_S3_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="299" mass="33024" checksum="B1553FD178B591C3" modified="2011-05-03" version="1">MIEKDFVTEGLKRTRIDEYLENELERAGYGGMEIQVTPLGTMVVVYAERPGMVIGRGGKTVRSITQNLKTGYGLENPQVEVKEVDVPELNPKIMAHKIAAMLQRGMHFRRVAYTALRRIMGAGAQGVEVTISGKIRGSRSACAKFSDGYIKKCGEPSIRFVRHGFATVQLKPGVLGINVRIMPPDVVLPDKVDILQIEVEKPETENVEVEAAPEKPETVESVVEEIVEEAAEEAEDIIEEIVEDVEEAVEEITEAIEEATEEEETPAKEAPKDEEAADESATEEVQETEENADKADKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB90</accession>
    <name>F0TB90_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps8</name>
        <name evidence="6" type="ordered locus">Metbo_0790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644392.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0790"/>
    <dbReference type="EnsemblBacteria" id="ADZ09041">
        <property type="protein sequence ID" value="ADZ09041"/>
        <property type="gene ID" value="Metbo_0790"/>
    </dbReference>
    <dbReference type="GeneID" id="10277238"/>
    <dbReference type="KEGG" id="mel:Metbo_0790"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="OrthoDB" id="91154at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_S8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="14810" checksum="554C9C56A8F489FE" modified="2011-05-03" version="1">MTLMDPLADALTNMRNNEMQGNKRCTIKPASKMIGHVLRTMQKEGYIGEFEFVDDDKAGQFIVELEGNINKCGVIKPRHAVKKDEFEKFEKRYLPSKNFGTIVITTPEGIMTHREAKEKGIGGRLLVYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB97</accession>
    <name>F0TB97_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl15</name>
        <name evidence="7" type="ordered locus">Metbo_0797</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644399.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0797"/>
    <dbReference type="EnsemblBacteria" id="ADZ09048">
        <property type="protein sequence ID" value="ADZ09048"/>
        <property type="gene ID" value="Metbo_0797"/>
    </dbReference>
    <dbReference type="GeneID" id="10277245"/>
    <dbReference type="KEGG" id="mel:Metbo_0797"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="OMA" id="WGRVGQH"/>
    <dbReference type="OrthoDB" id="121171at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_prot_L15/27a_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="6">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="15826" checksum="A37684338EAEEBC8" modified="2011-05-03" version="1">MIRKTRKICKLRGSRTVAGGCSKKRRGAGNRGGKGLAGGHKHHWSWMVKFDPKHFGKYGFKRPQGTSQVFNPVNVDFLDENSDKLLSQGVATEEDNKIIIDVTELGYNKVLGKGKISKALTIKAPQFSKSAVSKIENAGGEALTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF0</accession>
    <name>F0TBF0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ada_Zn_binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645570.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2000"/>
    <dbReference type="EnsemblBacteria" id="ADZ10219">
        <property type="protein sequence ID" value="ADZ10219"/>
        <property type="gene ID" value="Metbo_2000"/>
    </dbReference>
    <dbReference type="GeneID" id="10278460"/>
    <dbReference type="KEGG" id="mel:Metbo_2000"/>
    <dbReference type="eggNOG" id="arCOG05052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987638_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ada_Zn_binding" evidence="2">
        <location>
            <begin position="78"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13044" checksum="684B76E11CBFF521" modified="2011-05-03" version="1" precursor="true">MKKIATILVLFLIVGIIGISGCTSNDGNTSNQSYTNTTTQTPVTTQTTNTSTASNTSSPAPSVNTTSSSKSSSGPGNYIGNANTGKFHLASCRYISKMNEGNKIFFNSRESAISSGYVPCKVCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS1</accession>
    <name>F0TBS1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644499.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0899"/>
    <dbReference type="EnsemblBacteria" id="ADZ09148">
        <property type="protein sequence ID" value="ADZ09148"/>
        <property type="gene ID" value="Metbo_0899"/>
    </dbReference>
    <dbReference type="GeneID" id="10277348"/>
    <dbReference type="KEGG" id="mel:Metbo_0899"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_4_0_2"/>
    <dbReference type="OMA" id="AHMEEID"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="316" mass="36113" checksum="D5AD908D5028514B" modified="2011-05-03" version="1">MPDLTVIIPNFNGKHLLKQCFESLKIQNCSMDVIVVDNGSTDGSADYINLNYPEFKLVENRENLGFSKAVNQGIAASNTDFVMLLNNDVVLERCAIKNLLTCIKKDSEIFAAASKMLNSDELTIDDAGDEYTLLGWTKKVGNGKPETAYSKDREIFSACAGAAIYRRKVFDEIGYLDESFFAYMEDVDISYRARIHGYKCVYCSKAVVYHLGSATSGSKYNSFKIKLAARNNVYVPYKNMPWPQLLLNLIFLVIGYFIKYLFFLRKGYGNDYLNGLKEGLHSLDRVGKVEYKWDRLTNYLYIEWYLIKNTLHSIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC07</accession>
    <name>F0TC07_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645700.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2130"/>
    <dbReference type="EnsemblBacteria" id="ADZ10349">
        <property type="protein sequence ID" value="ADZ10349"/>
        <property type="gene ID" value="Metbo_2130"/>
    </dbReference>
    <dbReference type="GeneID" id="10278593"/>
    <dbReference type="KEGG" id="mel:Metbo_2130"/>
    <dbReference type="HOGENOM" id="CLU_1745568_0_0_2"/>
    <dbReference type="OrthoDB" id="129734at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17002" checksum="82D7C6645D75A4FF" modified="2011-05-03" version="1">MKKSVKLTGTFFIASLMILEIFTFMIPTDQLSSSAATYESQPDSKIINESQVIRRFNEIKTIKYDEVNMNCKNKSELFAGYLKSIGATDIYIVTVKHGSGTYSHEFVEWNGHYYDTCSSDTSYEIPMEEYSKKLYKLGFTGISVTSPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC16</accession>
    <name>F0TC16_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644518.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0918"/>
    <dbReference type="ESTHER" id="metla-f0tc16">
        <property type="family name" value="Asp2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09167">
        <property type="protein sequence ID" value="ADZ09167"/>
        <property type="gene ID" value="Metbo_0918"/>
    </dbReference>
    <dbReference type="GeneID" id="10277367"/>
    <dbReference type="KEGG" id="mel:Metbo_0918"/>
    <dbReference type="eggNOG" id="arCOG07416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071230_0_0_2"/>
    <dbReference type="OrthoDB" id="69240at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="30825" checksum="D2DA2494ED0B1C2E" modified="2011-05-03" version="1">MGFADYIKANEEVHKAGSEVKYLFCEKEGADKLIVTFPGFTNSGKFKYRYVRTLQDVNAHRLFLLDEFGVRGCYLLGHHRSFKIETVVMSLITSMLKKYGLKIDDVILQGSSKGGWMALYYGIKYRFGHVIAGGPQTKMGDFLIKEDVEIQPDEKIHVFSKVRVADYIAGGHEHGDLTYLNNLLYEQLYRDPEDFPMIYIHVGKGDSHFENHIKPFVTQLDINNIKYELDIQEYHEHNDLGIFYPKFLLKTLQTIDDKLIPQRMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC68</accession>
    <name>F0TC68_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0972"/>
    <dbReference type="EnsemblBacteria" id="ADZ09219">
        <property type="protein sequence ID" value="ADZ09219"/>
        <property type="gene ID" value="Metbo_0972"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0972"/>
    <dbReference type="eggNOG" id="arCOG01411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_27_1_2"/>
    <dbReference type="OMA" id="EAYMAAD"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="2">
        <location>
            <begin position="200"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="389" mass="45098" checksum="AD4D7820831EC26C" modified="2011-05-03" version="1">MLEIDYINGPKSDKMFGMYKYQMEIRKRLDVILNVIEYESIMQNLEKRLTPPNNTDAKAQKKSDENSKDSGIKNFLFNQARKSVQEIDRYRYRLIVKNSIHQDNIKHLTNQELAYLLNHMDLDKTVVTCYDLIPWVYENDHGKYWKNIMTGLRKSDRIITISEFSKMEIMKELNYPEERIEIVSVAVDHKVYHPKRDKTILKRFNIPENQKTILYVGSETPRMNLDFLLKSLSKLKKTYPDFKLIKVGDPQSFGAREHFLNTIKATGLEKNVIFTGYVAEEELPKWYNAADLLVYPCLYAGFGVPPLEAMACGTPVITSNTSSLPEVVGNAGVMVDPNDTDAMSKSMFEVLTNESRKEELVQRGLKRSKLFQWDESANTTGEIYRELDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC75</accession>
    <name>F0TC75_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0979"/>
    <dbReference type="EnsemblBacteria" id="ADZ09226">
        <property type="protein sequence ID" value="ADZ09226"/>
        <property type="gene ID" value="Metbo_0979"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0979"/>
    <dbReference type="HOGENOM" id="CLU_3039060_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="54" mass="6280" checksum="6A8391C58E32CB1C" modified="2011-05-03" version="1">MGMISLIDEDLDEDVDETIQHYARKKRETSKNKVFMPAIVIIIILVVLIVYFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD0</accession>
    <name>F0TCD0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo12</name>
        <name evidence="8" type="synonym">rpoP</name>
        <name evidence="9" type="ordered locus">Metbo_2182</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645748.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2182"/>
    <dbReference type="EnsemblBacteria" id="ADZ10397">
        <property type="protein sequence ID" value="ADZ10397"/>
        <property type="gene ID" value="Metbo_2182"/>
    </dbReference>
    <dbReference type="GeneID" id="10278645"/>
    <dbReference type="KEGG" id="mel:Metbo_2182"/>
    <dbReference type="eggNOG" id="arCOG04341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3227795_0_0_2"/>
    <dbReference type="OrthoDB" id="129924at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="43" mass="4996" checksum="7047D97AB5844BA9" modified="2011-05-03" version="1">MYKCGKCGTLVDIKGYTESKCPSCRYRILFKEIPPVRRSIKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD5</accession>
    <name>F0TCD5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">psmA</name>
        <name evidence="6" type="ordered locus">Metbo_2188</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3 4">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645753.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2188"/>
    <dbReference type="EnsemblBacteria" id="ADZ10402">
        <property type="protein sequence ID" value="ADZ10402"/>
        <property type="gene ID" value="Metbo_2188"/>
    </dbReference>
    <dbReference type="GeneID" id="10278651"/>
    <dbReference type="KEGG" id="mel:Metbo_2188"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="OMA" id="FQVEYAR"/>
    <dbReference type="OrthoDB" id="57654at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PROTEASOME_ALPHA_1" evidence="5">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="27502" checksum="B17C9A8CE517C411" modified="2011-05-03" version="1">MQPFQGAGYDRAITVFSPDGRLFQVEYAREAVKRGTTSLGVKSVEGIVLVVDKRPTSKLVEPKSIEKIFQIDDHIGAATSGLVADARALVEKARMESQINKITYNEPIRVDGLAKKICDMKQMYTQHGGVRPFGSALIIGGVNESGCKLFETDPSGALIEYKATAIGAGRQIAMDEFEKKYSEDIKLDEAIELALDAVYEATEGKTTVESVEIAIIDAKDKKFRKLADDEITEHVEELLIRKSKEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG6</accession>
    <name>F0TCG6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644594.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0996"/>
    <dbReference type="EnsemblBacteria" id="ADZ09243">
        <property type="protein sequence ID" value="ADZ09243"/>
        <property type="gene ID" value="Metbo_0996"/>
    </dbReference>
    <dbReference type="GeneID" id="10277445"/>
    <dbReference type="KEGG" id="mel:Metbo_0996"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OMA" id="LIVVHAY"/>
    <dbReference type="OrthoDB" id="99962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="14644" checksum="4CE1297707DAB84A" modified="2011-05-03" version="1">MFERIMFPTDGSEYAARSEDIVMEMAKKFGSTVVAVNIIDDKLIYPFEVLEDEGKSILSHVSERGQNEDVKVEEVLIVGSPTHDMEKIVKKTEADLVVIATHGKTGLEKLILGSVAESALKTVKVPVLLVKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK1</accession>
    <name>F0TCK1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Plasma-membrane proton-efflux P-type ATPase</fullName>
            <ecNumber evidence="10">3.6.3.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1031</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.6" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644629.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1031"/>
    <dbReference type="EnsemblBacteria" id="ADZ09278">
        <property type="protein sequence ID" value="ADZ09278"/>
        <property type="gene ID" value="Metbo_1031"/>
    </dbReference>
    <dbReference type="GeneID" id="10277480"/>
    <dbReference type="KEGG" id="mel:Metbo_1031"/>
    <dbReference type="eggNOG" id="arCOG01578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_4_2"/>
    <dbReference type="OrthoDB" id="17967at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="679"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="9">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="825" mass="91306" checksum="D9E926B13CBC2621" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP3</accession>
    <name>F0TCP3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2219"/>
    <dbReference type="EnsemblBacteria" id="ADZ10433">
        <property type="protein sequence ID" value="ADZ10433"/>
        <property type="gene ID" value="Metbo_2219"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2219"/>
    <dbReference type="eggNOG" id="arCOG06687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056316_0_0_2"/>
    <dbReference type="OMA" id="GIRVMCG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="428" mass="44601" checksum="F0E10A21DC34D90D" modified="2011-05-03" version="1" precursor="true">MEHIKGLKFMKRSNLVGILLFLMTVGMLVHTSAATMNVVVITDPTGQDPNGAAAGSQSWAENMFQSTFILSKEKHFCVLSGGEGNETPRLGAIVSAVNNLENGGTAASAATLANGFSGIRIMTGGPTIGAAVGGYFLAYVVVVSDNGTITITPYNGGLAVLPPGTKGAIIHLRNTPGNPQYGTADAVRLQTAEMMGQMIRDGYPATDVVAAAFKNVAINAGEKHGGGALNLVSGITTGDMFTPTELNTTGFSMDQPYSKISDNGWSVAYPEAANYEVSPIDGSNLTTVYAYQALQNAITVDSNTTSVAVYGTDAPGVASTTQEIVQASVSKHGFSGTQIAKDINSAISNGLLVGVNNVEASDINVNQNQKAVGVYFKALPDDRSSPPWNLPISSNILNLLGNMQTAIGIILVILVLFRSTLISSFLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ6</accession>
    <name>F0TCQ6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645797.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2232"/>
    <dbReference type="EnsemblBacteria" id="ADZ10446">
        <property type="protein sequence ID" value="ADZ10446"/>
        <property type="gene ID" value="Metbo_2232"/>
    </dbReference>
    <dbReference type="GeneID" id="10278698"/>
    <dbReference type="KEGG" id="mel:Metbo_2232"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_1_0_2"/>
    <dbReference type="OMA" id="DEWTWES"/>
    <dbReference type="OrthoDB" id="67420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="67"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="130"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="226"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="278" mass="31134" checksum="56E7499AC07C1911" modified="2011-05-03" version="1">MLVKEIMAKDIISVHVPGNRANALEIMRKKKVSGLPVVKNGTDQLVGVLTRTDLVENPDEEQIALIMTRDIITASPDDSVKTVAEKMINNNIRRIPIVEEGRLVGLVTASDLVNKALWKMEIQEPAEDYMIQNIPTSWEGTPLNVAFEIMRYYRLKVLLGLNNDGKLTGILTETDFIEESEVVSERTVHNTSVGTEGDKWSWDSKSVLYVIKNHLKFSDKKIKDVANTDLVIVTTKTSVQECANKMRQRNIEQIPVIDVEGDLVGLVRAVDLIKAIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR2</accession>
    <name>F0TCR2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2238"/>
    <dbReference type="EnsemblBacteria" id="ADZ10452">
        <property type="protein sequence ID" value="ADZ10452"/>
        <property type="gene ID" value="Metbo_2238"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2238"/>
    <dbReference type="eggNOG" id="arCOG01858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073842_1_0_2"/>
    <dbReference type="OMA" id="GRSKTHG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004454">
        <property type="entry name" value="HD-related"/>
    </dbReference>
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        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00295">
        <property type="entry name" value="TIGR00295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="19"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="18749" checksum="566471AFBFE3AA1B" modified="2011-05-03" version="1">MDITTCLKALNDYNCPPNVIAHSKAVSKKAVEIAKTYSEKTDATVDVEQVKYGALVHDIGRSKTHSIQHAVVGAEILRDLNFPECYINITLKHIGAGIPCEEAEELGLPKRDYIPSTIEEKIVAQADNLTSGTAEVDLTFVLKKWKESFGKNHPAIKRIKLLHEELLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT7</accession>
    <name>F0TCT7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase expression/formation protein HypD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2263"/>
    <dbReference type="EnsemblBacteria" id="ADZ10477">
        <property type="protein sequence ID" value="ADZ10477"/>
        <property type="gene ID" value="Metbo_2263"/>
    </dbReference>
    <dbReference type="GeneID" id="10278729"/>
    <dbReference type="KEGG" id="mel:Metbo_2263"/>
    <dbReference type="eggNOG" id="arCOG04428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048562_1_0_2"/>
    <dbReference type="OMA" id="HVCGSHE"/>
    <dbReference type="OrthoDB" id="24633at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11750">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
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    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="PTHR30149"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="352" mass="38973" checksum="D9C7AE5EA5B4F84F" modified="2011-05-03" version="1">MKNLSKEIIERIEKISRPVKIMHVCGSHEHTIMQYGIRTLIPEEVEIVAGPGCPVCCVPSREVDECLYLAREGVTIATFGDMLRVPGTKGTLADAKADGADVRIVYGVNNAVEMAQKTDNDVVFMAAGFETTAPTTAAELVNRPPENFSVISSHRLIPPALKFLIESGEVNLNALIEPGHVSTIIGTKPYESFSKDYGIPQVVAGFNPFDVLIAIYMVLKQFENGEAEVQNEYKRAVRDEGNVKAQELLEEVFFIKSREWRGFPEIPNSVYEVKPEFDEFNARKKFDIDIGESEPVVTGCICGPILRGVARPEECKLFKKECNPMNPVGACMVSKEGTCNIAFRYGSFDPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU8</accession>
    <name>F0TCU8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2274"/>
    <dbReference type="EnsemblBacteria" id="ADZ10488">
        <property type="protein sequence ID" value="ADZ10488"/>
        <property type="gene ID" value="Metbo_2274"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2274"/>
    <dbReference type="eggNOG" id="arCOG04838">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_213007_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="44" mass="5010" checksum="BEE5E3899B40A739" modified="2011-05-03" version="1">MADLKMKKLKTKRYKCLDCGNEFQGIGKRVVCPSCQSENVEESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV1</accession>
    <name>F0TCV1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10491">
        <property type="protein sequence ID" value="ADZ10491"/>
        <property type="gene ID" value="Metbo_2277"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2277"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_901980_0_0_2"/>
    <dbReference type="OMA" id="SNWIELQ"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="3">
        <location>
            <begin position="44"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="2">
        <location>
            <begin position="212"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="308" mass="33829" checksum="E324A20085BA9E8F" modified="2011-05-03" version="1" precursor="true">MRLIGYGGDRIKQKLFLSVLLLFGVALLLNVNSVSAANATGIHTSPKVVAVDPANNAIIQKSQNIKVKFNESIKSGNNYVELKHGNVLISTKKTVNGNTLTITPSTTLSTNYKYTLILHSGSVTSITGDKIGVYTTSFTVSQLTLAQMKDGLKRAQTFYNTNYRLPNYVSYGDTKISITTFQKIIATQGLKINTTTVVKSSNSDIDSSVATIMKGASKYSYSHSASTAEAMERNGAGDCWAMSDYLYKKMRAAGIHSRIIQYPTRYASNHRSVQYLENGSWVDAPYRTYFSTDMFNNTQSYGTVIAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV5</accession>
    <name>F0TCV5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyl-coenzyme M reductase subunit alpha</fullName>
            <ecNumber evidence="10">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metbo_2281</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="10">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645846.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2281"/>
    <dbReference type="EnsemblBacteria" id="ADZ10495">
        <property type="protein sequence ID" value="ADZ10495"/>
        <property type="gene ID" value="Metbo_2281"/>
    </dbReference>
    <dbReference type="GeneID" id="10278747"/>
    <dbReference type="KEGG" id="mel:Metbo_2281"/>
    <dbReference type="eggNOG" id="arCOG04857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="OMA" id="VGHQPEY"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_alpha_N" evidence="14">
        <location>
            <begin position="5"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_alpha" evidence="13">
        <location>
            <begin position="317"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Pros-methylhistidine" evidence="12">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="12">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ10495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="550" mass="60374" checksum="ED83130B0698702C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X4</accession>
    <name>F0T5X4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644718.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09367">
        <property type="protein sequence ID" value="ADZ09367"/>
        <property type="gene ID" value="Metbo_1122"/>
    </dbReference>
    <dbReference type="GeneID" id="10277572"/>
    <dbReference type="KEGG" id="mel:Metbo_1122"/>
    <dbReference type="HOGENOM" id="CLU_815378_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="38997" checksum="9892FD623CBB3E35" modified="2011-05-03" version="1">MTIPLLAADKDIMDIIIAIIQMITLIFLIIYVWKTYDMAVSTEKSAKVSQLTLQEMKETRDQEIAPYVVAYFEFENRIINLIVENVGKGLAKDVKIKFDPKLISSIGHDINEIAFIKEGIGSMPPKYKIKTFFDSTIGYYGIKNFPNKYNAKITYYGGLEESKRTTEHILDIGAYFNVLSSEEKGFNEVVKELEKISKAQESINKELSNYNDIFSNGIWINNPASPINGSNSNINDNRKAILSKLNEFKILWESVYKNEEEKFGSFYTNMGKRFSSIGMQILILSSYETKEELNEDLIKIGTKLFKLERIRFSWGIEKKFTKIGNEVSLLVDKVINEINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y0</accession>
    <name>F0T5Y0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1129"/>
    <dbReference type="EnsemblBacteria" id="ADZ09373">
        <property type="protein sequence ID" value="ADZ09373"/>
        <property type="gene ID" value="Metbo_1129"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1129"/>
    <dbReference type="HOGENOM" id="CLU_2911529_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="61" mass="7299" checksum="83A0DDE9E18C1007" modified="2011-05-03" version="1">MKKEKRVCLTLPQPIHEMIWKTSGKLKVNYVAESLLIEFMVDPDLQKRVKNRIRDGFKRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T628</accession>
    <name>F0T628_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein AfpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2321"/>
    <dbReference type="EnsemblBacteria" id="ADZ10535">
        <property type="protein sequence ID" value="ADZ10535"/>
        <property type="gene ID" value="Metbo_2321"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2321"/>
    <dbReference type="eggNOG" id="arCOG01705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014072">
        <property type="entry name" value="Archaeoflavo_AfpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02699">
        <property type="entry name" value="archaeo_AfpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="5"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="20982" checksum="FCF3A67BF51E84B0" modified="2011-05-03" version="1">MKKPKIAWGLTGAGDKIVETLKYMEKIKKEYDDRVDIELFVSKSGDQVLKYYGISTEVETHFDKVWTEINANAPFLAGNIQLGKYDFMLIAPASSNTVSKIAMRMGDTLISNAAIMGQKADVPLYILPSDYEEGVTITQLPDGNDLKISIREEDVEHIKKLAKMHETYVIKEPNDIHGIFRKYYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T674</accession>
    <name>F0T674_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane-flanked domain DUF304</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645932.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2368"/>
    <dbReference type="EnsemblBacteria" id="ADZ10581">
        <property type="protein sequence ID" value="ADZ10581"/>
        <property type="gene ID" value="Metbo_2368"/>
    </dbReference>
    <dbReference type="GeneID" id="10278834"/>
    <dbReference type="KEGG" id="mel:Metbo_2368"/>
    <dbReference type="eggNOG" id="arCOG04619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094459_0_0_2"/>
    <dbReference type="OMA" id="WDVISWR"/>
    <dbReference type="OrthoDB" id="112008at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_2" evidence="3">
        <location>
            <begin position="92"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="23994" checksum="D37078372E89438B" modified="2011-05-03" version="1">MFDRNKRSSIPGERILYKTRPRFVVSLKSAIIKFIIVLILVYFFTTLIKYAAMVQGRVGAFINIPFVEGTTDLIVIVILLLLVWILWNYLSWRSTYYTLTNQRVMLRSGVIRKKSAYMHYNKIQDIVVSQSVIERISYSGDIEVFGGHDNTSIILEDIPNPGEVEDTINRMIDGDEPEFETKAPKKTEEPKITGRSIMEEYDKKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T697</accession>
    <name>F0T697_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase A24A domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1168"/>
    <dbReference type="EnsemblBacteria" id="ADZ09412">
        <property type="protein sequence ID" value="ADZ09412"/>
        <property type="gene ID" value="Metbo_1168"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1168"/>
    <dbReference type="eggNOG" id="arCOG02300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716919_0_0_2"/>
    <dbReference type="OMA" id="WKDIIPS"/>
    <dbReference type="OrthoDB" id="62175at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_A24" evidence="2">
        <location>
            <begin position="12"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Arc_PepC" evidence="3">
        <location>
            <begin position="245"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="391" mass="44059" checksum="C782386485B1D023" modified="2011-05-03" version="1">MFVNIPLITIIIAVVACIYASYSDLKFGLIKNKLTFPLIAIGIILNGIYVLITNDIFLFLEGCFVTGLVFIVGYVFWKMGAWAGGDVKLFTAVAALIPFYATSYYPTIVTYQILGWQFPLLSLYQFPFTVIINSILSMLPFLLVFVFYIALKNKPHLISELFSPLKDYEKNLVLTLVISSSVTIAYYITFELKFQIIILTLILIYLLSAVISKIPNKIKAVLVSIVTVYALINNWEITITGIVFLFISINVIEIIKKLLTSVSKQALEEDYKIDELKEGMIPAYNLYENDDEIYEDKSTFFGRLKQGISSGDVSKLNGPQDKLIISTMAAGLNTENIEYLKELRDKKKMKDNLKVKRGVPFAPSILIGLLISLFIGDIAFIVEKILMAIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A8</accession>
    <name>F0T6A8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cysteine synthase A</fullName>
            <ecNumber evidence="2">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.47" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644774.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1179"/>
    <dbReference type="EnsemblBacteria" id="ADZ09423">
        <property type="protein sequence ID" value="ADZ09423"/>
        <property type="gene ID" value="Metbo_1179"/>
    </dbReference>
    <dbReference type="GeneID" id="10277629"/>
    <dbReference type="KEGG" id="mel:Metbo_1179"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="OMA" id="RTPVFKF"/>
    <dbReference type="OrthoDB" id="47519at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="1">
        <location>
            <begin position="15"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="34597" checksum="638A2B8876C1320D" modified="2011-05-03" version="1">MVNIPKLTRGIANDITETIGNTPLVRLNKLTEGSKAEVLVKVESFNPLSSVKDRIGVAMIEAGEEAGVIKENTILIEPTSGNTGIALAFVAAAKGYKLILTMPDTMSIERRKLLALLGAEIVLTPGANGMPGAVAKAEELAAEIPGAVMPQQFKNIANPKIHRETTAQEIWRDTDGKVDVLVSGTGTGGTITGVATALKELKPEFKAVAVEPVTSPVLSGGSPGPHKIQGIGPGFIPDVLQTEIIDEIVQVSDDDAAQTLLKLAREEGILAGISSGAAVFAALQLAKKDEYAGKQIVVILPDTGERYLSMDWVFEDIFKSSESSDFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C6</accession>
    <name>F0T6C6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644792.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1197"/>
    <dbReference type="EnsemblBacteria" id="ADZ09441">
        <property type="protein sequence ID" value="ADZ09441"/>
        <property type="gene ID" value="Metbo_1197"/>
    </dbReference>
    <dbReference type="GeneID" id="10277647"/>
    <dbReference type="KEGG" id="mel:Metbo_1197"/>
    <dbReference type="eggNOG" id="arCOG10178">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082370_0_0_2"/>
    <dbReference type="OMA" id="FGWLYWK"/>
    <dbReference type="OrthoDB" id="93058at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="255" mass="27813" checksum="29733A2E577A800D" modified="2011-05-03" version="1" precursor="true">MNTNINWKLFGILLIASIISAVLVLPYTLALSPGLAAVFTPFVLIAQMIQTLVIFSVAIFLGLILAKRVGFSLPILEGWLEGREVGSYFRSILGISIGLGLLSGVLIVVLSLFFTPVTATFQNVELSIPLWKGFLASFYGGISEEIVMRLFLMTLIVWIIFKIKKTEDGKPNSLGIWIAIVLSAVIFGLGHLPITGSITSITPLIIGRAVLLNGVGGIVFGWLYWKKGLESAMMGHFSADIVLHVIYPFFLMLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C8</accession>
    <name>F0T6C8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644794.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1200"/>
    <dbReference type="EnsemblBacteria" id="ADZ09443">
        <property type="protein sequence ID" value="ADZ09443"/>
        <property type="gene ID" value="Metbo_1200"/>
    </dbReference>
    <dbReference type="GeneID" id="10277650"/>
    <dbReference type="KEGG" id="mel:Metbo_1200"/>
    <dbReference type="eggNOG" id="arCOG01803">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926261_0_0_2"/>
    <dbReference type="OMA" id="NPSYGIV"/>
    <dbReference type="OrthoDB" id="78272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="33740" checksum="CCDDC227B67C01E2" modified="2011-05-03" version="1">MRIGYLSTIYHTSFILKSGNIGYKNSDETEWQLFPTGPAIMEAFKDKEIDLGYVGLPPVMIGIQNGMKLKCVAGGHIEGTVMIAKEEYDSYDDLGDLESVLKQFKGKSIGTPTRGSIHDVIIRNLIVNSDVSINNYPWADFIPDAISENEIAAGVGTPSLATVADMQLKTKIIIPPNMLWPYNPSYGIVVQEDMIKEDEEFILSFLKAHEEACNLIRNYPEQAADIAAEEMGVVDKDFVLKTYSISPRYCAKIPEEYIKSTMDFLPVLKKLGYMSTDLNPEDIFELKYINQVHKEPAHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F6</accession>
    <name>F0T6F6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polyketide cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645941.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2377"/>
    <dbReference type="EnsemblBacteria" id="ADZ10590">
        <property type="protein sequence ID" value="ADZ10590"/>
        <property type="gene ID" value="Metbo_2377"/>
    </dbReference>
    <dbReference type="GeneID" id="10278843"/>
    <dbReference type="KEGG" id="mel:Metbo_2377"/>
    <dbReference type="eggNOG" id="arCOG06222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069867_5_1_2"/>
    <dbReference type="OMA" id="CIRWTGP"/>
    <dbReference type="OrthoDB" id="93730at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="16071" checksum="2FC6E5727BCFA5AE" modified="2011-05-03" version="1">MKINENAPASACAEVKINADPELVWNVLIDVKNWPNWNPDVKNVVMDQKFAVGSQFKWKAGPGTISSKLQEIEKPKLLVWTGKTMGIHAVHVWKLKPIDSQTLISSEESWDGLIVRILSGSMQKTLKKSTESGLNYLKQEIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H3</accession>
    <name>F0T6H3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L30e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl30e</name>
        <name evidence="7" type="ordered locus">Metbo_2394</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645958.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2394"/>
    <dbReference type="EnsemblBacteria" id="ADZ10607">
        <property type="protein sequence ID" value="ADZ10607"/>
        <property type="gene ID" value="Metbo_2394"/>
    </dbReference>
    <dbReference type="GeneID" id="10278861"/>
    <dbReference type="KEGG" id="mel:Metbo_2394"/>
    <dbReference type="eggNOG" id="arCOG01752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="OMA" id="RPHKIMA"/>
    <dbReference type="OrthoDB" id="117214at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="L30/L30e/YlxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_L30e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_L30e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="PTHR11449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449:SF1">
        <property type="entry name" value="PTHR11449:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="6">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="10577" checksum="706414ED8D8F9F0F" modified="2011-05-03" version="1">MDIDRGIRVAVDTGNVTLGSDKSIQELKLGKGKLVIIARNCPEDIREDVEQYSKLSDITVYTYDGTSVDLGSVCGKPFTVATLIIQDPGDSTILEIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P1</accession>
    <name>F0T6P1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643625.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0021"/>
    <dbReference type="EnsemblBacteria" id="ADZ08274">
        <property type="protein sequence ID" value="ADZ08274"/>
        <property type="gene ID" value="Metbo_0021"/>
    </dbReference>
    <dbReference type="GeneID" id="10276443"/>
    <dbReference type="KEGG" id="mel:Metbo_0021"/>
    <dbReference type="eggNOG" id="arCOG03003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129810_1_1_2"/>
    <dbReference type="OMA" id="PLKILCC"/>
    <dbReference type="OrthoDB" id="105481at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="1">
        <location>
            <begin position="12"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13756" checksum="A38573D99BE59D1D" modified="2011-05-03" version="1">MLIKDTKSSNYFNAVDETVLAELLHPKNDGVKLDFSIAHAVLKPGKSSLPHILRESVEVYFVLEGQGQMHIDHEVETVESGQAVYIPPKKNQWIKNTGKEDLKFLCIVSPPWNESDEELSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P9</accession>
    <name>F0T6P9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0029"/>
    <dbReference type="EnsemblBacteria" id="ADZ08282">
        <property type="protein sequence ID" value="ADZ08282"/>
        <property type="gene ID" value="Metbo_0029"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0029"/>
    <dbReference type="HOGENOM" id="CLU_2191045_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="11628" checksum="3A0650BD9D1137C5" modified="2011-05-03" version="1" precursor="true">MKKAALICAMVLIVSIVPAFALNQTSTNTTAQVQNHSGTCDQTQAHSHDKNQNGQKNCTTTKCTTSDGDQHKYQYGLKNTANTSTDNHYKYQNGQKNGASGATCSQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R5</accession>
    <name>F0T6R5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Riboflavin kinase</fullName>
            <ecNumber evidence="5">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Metbo_0045</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AL-21</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CTP + riboflavin = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0045"/>
    <dbReference type="EnsemblBacteria" id="ADZ08298">
        <property type="protein sequence ID" value="ADZ08298"/>
        <property type="gene ID" value="Metbo_0045"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0045"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140165_0_0_2"/>
    <dbReference type="OMA" id="PYEGTLN"/>
    <dbReference type="OrthoDB" id="91297at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="PTHR40706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP-dep_RFKase" evidence="17">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ADZ08298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13773" checksum="F776F50D15A431AC" modified="2011-05-03" version="1">MGKIVSGMGKGTFFMSQDFYKAQFQEKVHFTPFEGTLNLKIDSKSIDSMKTIPKNKFGLIHGEGKFGDVKFIKATINNEIYGALVFPAKSEHNEDVLEFITDKNLRKLFKFQDGDEVTVILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V0</accession>
    <name>F0T6V0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644821.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1228"/>
    <dbReference type="EnsemblBacteria" id="ADZ09470">
        <property type="protein sequence ID" value="ADZ09470"/>
        <property type="gene ID" value="Metbo_1228"/>
    </dbReference>
    <dbReference type="GeneID" id="10277679"/>
    <dbReference type="KEGG" id="mel:Metbo_1228"/>
    <dbReference type="eggNOG" id="arCOG12992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047695_0_0_2"/>
    <dbReference type="OMA" id="IRDDLHC"/>
    <dbReference type="OrthoDB" id="134492at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="337" mass="38761" checksum="C4B01E679F979F59" modified="2011-05-03" version="1">MNRRGVCYDVGRVMMDKNFRPEFDDDIVKQELEIIKEDLHCNVVKICGKDVKRLMRASEFALKLGLEVWVSPELWDKNPEDTLEYIVEAAVETEKLRLIYEGKVVFIMGTELSLFMNGILNGENFMERLQSPDFMEIVTNGKHNTSLNEFLVKGNALVRNVFNGPVTYASAPFEAVNWNIFDFVCVDIYRDAKIKEEYPKILQSFARFGKPVVIGEFGCCTYEGAEDLGGWAWNILDELNSEELNGNYVRNEAVQAFELTEQLNILENSGVDGGFVFTFVQPTFKHSKDPCRDLDMASYSLVKTLCEGHGQTYKNMNWEPKESFYALSEFYDELEYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V5</accession>
    <name>F0T6V5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644826.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1233"/>
    <dbReference type="EnsemblBacteria" id="ADZ09475">
        <property type="protein sequence ID" value="ADZ09475"/>
        <property type="gene ID" value="Metbo_1233"/>
    </dbReference>
    <dbReference type="GeneID" id="10277684"/>
    <dbReference type="KEGG" id="mel:Metbo_1233"/>
    <dbReference type="eggNOG" id="arCOG15021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243846_0_0_2"/>
    <dbReference type="OrthoDB" id="120157at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="11918" checksum="E5864CE03876EEF8" modified="2011-05-03" version="1">MRNEANISYISSMDVKDEKGNILGAVCVSPAKEMGKRDIILMDEQAGSFSVRSTTELINMLSKKNVPFEERKQVLDFLAERLRVLEQEITLNTLKSLNDAKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X6</accession>
    <name>F0T6X6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1254</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644847.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1254"/>
    <dbReference type="EnsemblBacteria" id="ADZ09496">
        <property type="protein sequence ID" value="ADZ09496"/>
        <property type="gene ID" value="Metbo_1254"/>
    </dbReference>
    <dbReference type="GeneID" id="10277705"/>
    <dbReference type="KEGG" id="mel:Metbo_1254"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="ILNVMII"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="18"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="252"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="350" mass="37919" checksum="423871DB18B431F8" modified="2011-05-03" version="1" precursor="true">MNLYKLAFKNIRRKKLRSALTALGIIIGAATIVVLLGIGAGATSEVQEQTNQYMYDVAITSSSSSGNLLMDPQTVSKIKNMSNLYDFREVTVFSQDINNTRVNFEGVNDWKQVKGINGTTGVVINKDTADKLGLHIGDRLTVNDQQLTITGISKPEQATYVYLDQKTAENITGNKVSAIYARSYDNPNSTVDEVEKQVTGVSAVTKSDKVAQLQENTNQAMLFIGIIAGIALVVGVISVINTMMMSVMERTRELGVLKAIGFTNWELKGSILFESGLLGFLGSILGVILGIIGIIVFANMLHFTDYIPQMMPLWLIGGVIVGSTLLCLLAGLYPAISASKLNVVEALRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y7</accession>
    <name>F0T6Y7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="4">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name evidence="5" type="ordered locus">Metbo_1265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644858.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1265"/>
    <dbReference type="EnsemblBacteria" id="ADZ09507">
        <property type="protein sequence ID" value="ADZ09507"/>
        <property type="gene ID" value="Metbo_1265"/>
    </dbReference>
    <dbReference type="GeneID" id="10277716"/>
    <dbReference type="KEGG" id="mel:Metbo_1265"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OMA" id="GIVMNWT"/>
    <dbReference type="OrthoDB" id="61905at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="29223" checksum="C62514E602BF80F3" modified="2011-05-03" version="1">MAIFAKASEKDVTKAIVSEFAEEFLEYVESDVVIIGAGPSGLIAAKRLAESGVKVLLIESNNYLGGGFWIGGYLMNKLTVRAPGQKILDEIGVPYKEVQEGLFVADGPHACSKLIGATMDAGAKVINMTKFDDVVVRKDKVGGVVINWTPVSALPRAITCVDPVALESKIVVDATGHDAVVIKSLEQRGLIEIPGFEGMWVEKSEDEIVENTKQVYPGVYVTGMAVATTCGNTRMGPTFGGMLLSGEKVAELIINDLKVDVKVEGTASNKATSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T717</accession>
    <name>F0T717_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644888.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1295"/>
    <dbReference type="EnsemblBacteria" id="ADZ09537">
        <property type="protein sequence ID" value="ADZ09537"/>
        <property type="gene ID" value="Metbo_1295"/>
    </dbReference>
    <dbReference type="GeneID" id="24964547"/>
    <dbReference type="KEGG" id="mel:Metbo_1295"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_6_0_2"/>
    <dbReference type="OrthoDB" id="135567at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="169"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="31651" checksum="0F6253B6A88FBCC4" modified="2011-05-03" version="1">MPVVESNEEMKRNMRRIKKEEAGSKKMETDSSSKEADTKQQINVKVDEVKDSASDKKDSIQGKMDESKKVLGKKVDKTKEDVSDKKDEINVKLENVKDKASQKKDELNQGLEDTVDEVENQKQKLEKESEKQGMSPAEKILNDIVTRFRQGSDQINDAIVDRSEESKTSKKDLIKKPLIDVLETNDTIYLVADISGIKKDEIELGISKNSVEITAVFKDGPEIEGAKFAQKERNYGETHRKVMLSTDVKINEAKAKFKDCTLTITLPKKVEDLTKIEIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T739</accession>
    <name>F0T739_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10673">
        <property type="protein sequence ID" value="ADZ10673"/>
        <property type="gene ID" value="Metbo_2460"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2460"/>
    <dbReference type="eggNOG" id="arCOG09724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195000_0_0_2"/>
    <dbReference type="OMA" id="HETPCEG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="7520" checksum="BF0AFE1DC112458F" modified="2011-05-03" version="1">MNSEKINNEKACNDSENEKPEKIEKCYFCLKEFDINKDDSSHYKFGKYPMCAYCSEFYGFYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E2</accession>
    <name>F0T7E2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">Metbo_0118</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643722.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0118"/>
    <dbReference type="EnsemblBacteria" id="ADZ08371">
        <property type="protein sequence ID" value="ADZ08371"/>
        <property type="gene ID" value="Metbo_0118"/>
    </dbReference>
    <dbReference type="GeneID" id="10276543"/>
    <dbReference type="KEGG" id="mel:Metbo_0118"/>
    <dbReference type="eggNOG" id="arCOG02255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_3_0_2"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="OrthoDB" id="61864at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="222"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="232"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="292" mass="32188" checksum="08C9D2A46156ACEA" modified="2011-05-03" version="1">MSERTLVTFSKMHGLGNDYVVIDESKKIIVPEDKKPEICEEICRRGFSVGADGVIFVTPASNEEADIRFRIFNSDGSEAEMCGNGIRCFAKYVYENEILQNKEMLVETLGGIKEIELDVMGGEVRSSSVDMGIPTFKSVEIPMKTEKEEFLDAELDVNGTTVNLTAVNVGNPHAVIFTENIEEIDLNVMGPSIENHPAFPEKTNVHFVSVINRNEIEMITWERGAGFTLACGTGATASVMAGYKLGKLDEEVEVHLPGGELLIIVYEMGEETTLFMEGEAVLVFDGVMELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E9</accession>
    <name>F0T7E9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-(Apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643729.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0125"/>
    <dbReference type="EnsemblBacteria" id="ADZ08378">
        <property type="protein sequence ID" value="ADZ08378"/>
        <property type="gene ID" value="Metbo_0125"/>
    </dbReference>
    <dbReference type="GeneID" id="10276550"/>
    <dbReference type="KEGG" id="mel:Metbo_0125"/>
    <dbReference type="eggNOG" id="arCOG00464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027543_3_0_2"/>
    <dbReference type="OMA" id="YAQTYVF"/>
    <dbReference type="OrthoDB" id="83981at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037046">
        <property type="entry name" value="AlkA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="132"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="38033" checksum="F51B38966D811DFC" modified="2011-05-03" version="1">MKIGFEIDPKEYKGPFNLDLTINSGQTSQPAWLMKDGYFQELLRFDNKLCLIKIQQENVEGTLNIEAETEADLELESIRTKLMDIFSLNDDLNDFYDFLRKDSELKPTIDFCSGLRLFKAHDPFECIISSISSANCSIIRWNRSIRDIKSKWGEEYNSNSNSWYTFPSPDILKNVPEHDLEEMDRCETNLPDDHSFEKNLKSCGVGYRAKFMIKTAEIVQNQINIDAIHKMSYNNAFETMLDLPGVGPKVADCILFYGFGFKEAFPVDVWIGRIVSEMYFQGKEIKPLKAREFGMKKFANRAGYVQLYMFHYARKSGLLDRIRQLNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G3</accession>
    <name>F0T7G3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CoA-disulfide reductase</fullName>
            <ecNumber evidence="3">1.8.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0139</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643743.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0139"/>
    <dbReference type="EnsemblBacteria" id="ADZ08392">
        <property type="protein sequence ID" value="ADZ08392"/>
        <property type="gene ID" value="Metbo_0139"/>
    </dbReference>
    <dbReference type="GeneID" id="10276565"/>
    <dbReference type="KEGG" id="mel:Metbo_0139"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OMA" id="ACGIPYW"/>
    <dbReference type="OrthoDB" id="40511at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050451">
        <property type="term" value="F:CoA-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="1">
        <location>
            <begin position="335"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="47897" checksum="B0E64BD3484B18E9" modified="2011-05-03" version="1">MRVVVIGSGAGGLTVASNLRKYSKEVEITVFTMEDKVAYSPCAIPYVIGGDIDSFEEIVMHTPEYYGKHGINIVTESEVLEVASSQNLIKYRLINGDESAPELITLKYDYLVIATGGYPFIPPVEGNDLEGVFKVRTIEDGAKIKEWSKTSSKAVIVGAGAIGLELGYGLKQIGIEVSVTEMLPQVLPRSLDPDMALKVQEYLEGQGVRIILNNALTKIVGVDVAEEAVVGDIKMDADLVILSTGVRPSIELAKQAGCNIGDMGVVVNERMETSVSNIYAVGDCVEVHDGITGQKTLSPFGTTAVRQGKVAAKNLAGKDAVFRPVLNSVVSKIGELEVGAVGLTEESAKMNGIEVVVGKSQALTRARYYPGCKPIDTKLICGLDGTILGCQIIAKETVAERVDTMAMAIAKQVKCNEITEMEFSYAPPVSMVVDPAVMAAEDALKKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I6</accession>
    <name>F0T7I6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644905.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1312"/>
    <dbReference type="EnsemblBacteria" id="ADZ09554">
        <property type="protein sequence ID" value="ADZ09554"/>
        <property type="gene ID" value="Metbo_1312"/>
    </dbReference>
    <dbReference type="GeneID" id="10277763"/>
    <dbReference type="KEGG" id="mel:Metbo_1312"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="CQRCIVL"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="35251" checksum="D93A5FB523B42541" modified="2011-05-03" version="1">MNEYVMEAQDLTKKFGDFLAVDKLNLKIRKGEVLGFLGPNGAGKTTSINMMVGLLSPSSGKVLVNNSDVEEMDKKTIGICPQELVLWENLTCNENLKLMGDMYTVPKAILVERIEKLLRDLYLTEKADTRVSNLSGGMKRRLNMAMAVIHEPEIVLLDEPSEGLDPQSRRVLWSYIRSLRDDHQKTVILTTHLMDEADRLSDRVAIIDHGKLLKLDTPENLKKEIGEGDIIELTIPDKDNKKYVMNYLETLEDTISVVEVDGKINLRAFNAVGKLPKIVAELEKSNIQIDDLSVRQNTLEDVFIELTGTGLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J7</accession>
    <name>F0T7J7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09565">
        <property type="protein sequence ID" value="ADZ09565"/>
        <property type="gene ID" value="Metbo_1323"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1323"/>
    <dbReference type="HOGENOM" id="CLU_2165283_0_0_2"/>
    <dbReference type="OrthoDB" id="137739at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="110" mass="13147" checksum="1267AEC1ECB20CC7" modified="2011-05-03" version="1">MVNKNLNLEHTELVKDVPFEARVFLYFSIENKFGEDLTRLHENLKVWNFEHSDAKAYYSKAVERDNKTYFAVTIEFDYPTYLDEKVVNGLIEETKQNFTDFFHQKIEGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q8</accession>
    <name>F0T7Q8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646091.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2527"/>
    <dbReference type="EnsemblBacteria" id="ADZ10740">
        <property type="protein sequence ID" value="ADZ10740"/>
        <property type="gene ID" value="Metbo_2527"/>
    </dbReference>
    <dbReference type="GeneID" id="10279001"/>
    <dbReference type="KEGG" id="mel:Metbo_2527"/>
    <dbReference type="eggNOG" id="arCOG10135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121775_0_0_2"/>
    <dbReference type="OMA" id="CGSCTTW"/>
    <dbReference type="OrthoDB" id="82900at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="172" mass="18647" checksum="8C704C9F326E70FD" modified="2011-05-03" version="1">MIVKVVEKDVMDPVAETGDHKIELNKVDPDFKKLTLFNNTKPGADVILDYLGENLGKSEVIRVKKPAGAPATQNQLEKAALGEVVILGLGDCGSCSSWVILDAIRLEKIGVPTISICSTSFSEFSHELAKAHGAEDLNIVTVQHPIAGTKTSEIHDKTRKMMPLIKELLGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V5</accession>
    <name>F0T7V5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643793.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0190"/>
    <dbReference type="EnsemblBacteria" id="ADZ08442">
        <property type="protein sequence ID" value="ADZ08442"/>
        <property type="gene ID" value="Metbo_0190"/>
    </dbReference>
    <dbReference type="GeneID" id="10276616"/>
    <dbReference type="KEGG" id="mel:Metbo_0190"/>
    <dbReference type="eggNOG" id="arCOG02151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151731_0_0_2"/>
    <dbReference type="OMA" id="WMETVQK"/>
    <dbReference type="OrthoDB" id="111942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="15"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11553" checksum="1ABC90199A2DFCEF" modified="2011-05-03" version="1">MNENPFEILKQEFPEIAASLGELVEAQKSFKGIDNKTKQLLNLAIQTANRNPTGVQLHASMAKMEGASREEVLGAVVLNLHHSGLASVMECLPKAIKGFESHTEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X2</accession>
    <name>F0T7X2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L44e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl44e</name>
        <name evidence="3" type="ordered locus">Metbo_0207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643810.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0207"/>
    <dbReference type="EnsemblBacteria" id="ADZ08459">
        <property type="protein sequence ID" value="ADZ08459"/>
        <property type="gene ID" value="Metbo_0207"/>
    </dbReference>
    <dbReference type="GeneID" id="10276633"/>
    <dbReference type="KEGG" id="mel:Metbo_0207"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="OMA" id="CKKHTIH"/>
    <dbReference type="OrthoDB" id="106603at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_L44e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="PTHR10369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="93" mass="11048" checksum="450E5420ED7ED6CD" modified="2011-05-03" version="1">MKIPKERRTYCPSCKKHTIHEVLESKRRKASELKWGQRQFRRVTSGYRGYPRPLPSRNKPIKKLDLRYKCKECGKSHIKRSSFRAGKVEFVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X3</accession>
    <name>F0T7X3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3 4">30S ribosomal protein S27e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps27e</name>
        <name evidence="5" type="ordered locus">Metbo_0208</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3 4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0208"/>
    <dbReference type="EnsemblBacteria" id="ADZ08460">
        <property type="protein sequence ID" value="ADZ08460"/>
        <property type="gene ID" value="Metbo_0208"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0208"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OMA" id="DCENEQV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_S27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_S27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="3">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7011" checksum="BF7F9AB833FBC680" modified="2011-05-03" version="1">MIEVANYRSNKSNFLKVKCVDCGNQQVVFDHAASQVQCIICGKTLVKPKGGKSEITAQIIEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z3</accession>
    <name>F0T7Z3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644970.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1379"/>
    <dbReference type="EnsemblBacteria" id="ADZ09619">
        <property type="protein sequence ID" value="ADZ09619"/>
        <property type="gene ID" value="Metbo_1379"/>
    </dbReference>
    <dbReference type="GeneID" id="10277830"/>
    <dbReference type="KEGG" id="mel:Metbo_1379"/>
    <dbReference type="eggNOG" id="arCOG00474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_2"/>
    <dbReference type="OMA" id="KTVYMAV"/>
    <dbReference type="OrthoDB" id="88805at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="PTHR13017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="22718" checksum="47FE9B983457F7D8" modified="2011-05-03" version="1">MDQNKEELRNMVYTRLIENHISTPHYIGRIPDFKGSEKAADNLRSTMEWKISEIMFSSPDTAQRRVRENALKDGKILIMVTPKIENGYLKLEAQDLKGREREASTIEGAFKYGKKIEQLPRVDLVVEGSVAVDKNGRRLGKGGGYGDREIEYLKRTGSITPNTPIVSTVHDLQIVETVPTEKHDQKINMIVTPEKILKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z5</accession>
    <name>F0T7Z5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional deaminase-reductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644972.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1381"/>
    <dbReference type="EnsemblBacteria" id="ADZ09621">
        <property type="protein sequence ID" value="ADZ09621"/>
        <property type="gene ID" value="Metbo_1381"/>
    </dbReference>
    <dbReference type="GeneID" id="10277832"/>
    <dbReference type="KEGG" id="mel:Metbo_1381"/>
    <dbReference type="eggNOG" id="arCOG01490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_4_2_2"/>
    <dbReference type="OMA" id="GYIAKPN"/>
    <dbReference type="OrthoDB" id="80798at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="6"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19981" checksum="30456104E00E1C1F" modified="2011-05-03" version="1">MNYVYIAISLDGFIARPDGGLDWLENLDDPEDDYGFSDFIDKIDALVMGRKTFESVLKFDEWPFTKPVFVLSTTIQTVPNKLRGKVEVLNGHPKKIVEELNSKEFNNLYIDGGQTIQRFLKEDLIHEMIITTASVILGEGIPLFNGISGDIKFKCERVEVLNPYLVKHYYERDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T835</accession>
    <name>F0T835_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
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                <person name="Imachi H."/>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643878.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0275"/>
    <dbReference type="EnsemblBacteria" id="ADZ08527">
        <property type="protein sequence ID" value="ADZ08527"/>
        <property type="gene ID" value="Metbo_0275"/>
    </dbReference>
    <dbReference type="GeneID" id="10276707"/>
    <dbReference type="KEGG" id="mel:Metbo_0275"/>
    <dbReference type="eggNOG" id="arCOG04876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162437_0_0_2"/>
    <dbReference type="OMA" id="KPFPYRN"/>
    <dbReference type="OrthoDB" id="113300at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019597">
        <property type="entry name" value="Energy-convert_hydgase-B_suP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10622">
        <property type="entry name" value="Ehbp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10431" checksum="0E3FEEF4D752FF26" modified="2011-05-03" version="1">MKFVVSPMHIVSVGGYIVETQFPYRNLIVVNPTEEPIKMEIPVFNEEWIEEHRQLGLEITPITEKDSFLSNFRRAKLKLDQLKKEKETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C1</accession>
    <name>F0T8C1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643884.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0281"/>
    <dbReference type="EnsemblBacteria" id="ADZ08533">
        <property type="protein sequence ID" value="ADZ08533"/>
        <property type="gene ID" value="Metbo_0281"/>
    </dbReference>
    <dbReference type="GeneID" id="10276713"/>
    <dbReference type="KEGG" id="mel:Metbo_0281"/>
    <dbReference type="eggNOG" id="arCOG00813">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264587_0_0_2"/>
    <dbReference type="OMA" id="YTQQFIK"/>
    <dbReference type="OrthoDB" id="89825at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040537">
        <property type="entry name" value="DUF5612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015832">
        <property type="entry name" value="UCP006363_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18462">
        <property type="entry name" value="DUF5612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006363">
        <property type="entry name" value="UCP006363_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="23510" checksum="7C24F36E0A4B6245" modified="2011-05-03" version="1">MKEFAITIKAHNKPGVLRDITEMMAKCGINISYTHLFVEKDESASVYMELEDVKDIETLAHNILTHGNVDDVITHRSLGEIYGKRIIIIGGGAQVAMVAQGAITEADRHNIRGERISVDTIPLVGEQELAEAVSAVARLPRIGALVLAGSLMGGKISDAIEDIKEEHDVIVISLNMPGSVTEKADLVVTDPVQAGVMAVMAIADTALFDIKKIGKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E3</accession>
    <name>F0T8E3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyruvate ferredoxin/flavodoxin oxidoreductase, beta subunit</fullName>
            <ecNumber evidence="6">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1457</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645045.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1457"/>
    <dbReference type="EnsemblBacteria" id="ADZ09694">
        <property type="protein sequence ID" value="ADZ09694"/>
        <property type="gene ID" value="Metbo_1457"/>
    </dbReference>
    <dbReference type="GeneID" id="10277908"/>
    <dbReference type="KEGG" id="mel:Metbo_1457"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OMA" id="CTHGIIM"/>
    <dbReference type="OrthoDB" id="43666at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="54"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="PFO_beta_C" evidence="5">
        <location>
            <begin position="199"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="32253" checksum="57E271467FA09788" modified="2011-05-03" version="1">MKPENFDVKGADVSWCPGCGNFSILSNLKNVLADLEVDPENLVLVSGIGQAGKLPHFLRSNTYIGLHGRALSPATAIKAVNRGLKVIVATGDGDMYGEGGNHFLHTIRRNPDITCIVHDNMVYGLTKGQASPTTRLGMKTTYQVNGVSEEPFNPLTVAIALDASFVARTFSGDPEHLQKTLKSAINHEGYSLVDVFQPCVSFNKINTFQWFKENTYYLEESHDHYNKAEALRRAEETDKYPLGIFYINKQKKTFEETLNVYKNDDGTLHDRQVDKKKLNDLINSRRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F7</accession>
    <name>F0T8F7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="13" type="ordered locus">Metbo_1472</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645059.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1472"/>
    <dbReference type="EnsemblBacteria" id="ADZ09708">
        <property type="protein sequence ID" value="ADZ09708"/>
        <property type="gene ID" value="Metbo_1472"/>
    </dbReference>
    <dbReference type="GeneID" id="10277923"/>
    <dbReference type="KEGG" id="mel:Metbo_1472"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_2_1_2"/>
    <dbReference type="OMA" id="FNAFCIV"/>
    <dbReference type="OrthoDB" id="62484at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="12">
        <location>
            <begin position="80"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="41479" checksum="8535E2A766716983" modified="2011-05-03" version="1">MDPIYSNIAANKRWTYLFFLFYALMLGAIGYTMGIYFGYPIFGIGIAALVFILAFLVSYYGGQGMVTRMAGAKEVSHDDEPYLYNTVDALSIAAGIPMPKLYMINTDIPNAFAAGRSSKNSSITVTKGLLETLDRLELEGVIAHEISHIKNYDVLLSTVAIVLAGTIVFLGFTVRYSAYGGLFRGAKGQNNPAGIAILVILVLLIILAPIFAQLLKFAISRKREFLADASGADLTGYPEGLASALEKISTMQKPKSKIQNDALNSIYIVSPAIGKKGFSNLYSTHPPTGERVKRLREMEFIDQKHVKPEAANNTSTNQESNSNNMGFVAESAHTPENKAGNGYLVCNKCEGYYELQPGESPDDFTDECECGGKLEYRDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G3</accession>
    <name>F0T8G3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645065.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09714">
        <property type="protein sequence ID" value="ADZ09714"/>
        <property type="gene ID" value="Metbo_1480"/>
    </dbReference>
    <dbReference type="GeneID" id="10277931"/>
    <dbReference type="KEGG" id="mel:Metbo_1480"/>
    <dbReference type="eggNOG" id="arCOG06115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425098_0_0_2"/>
    <dbReference type="OrthoDB" id="135811at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="21910" checksum="385255DFE7C85BB8" modified="2011-05-03" version="1">MKQNVDKKFNEHKKIMMKKQSGSFKRQFNLQIRETSMGTFGHDRANFNGAVVQVAEDKLIITKKGTFTGRDRGNITLRYLDMISLDEDRGWVLTTVQIRMDGNHTVNLRSNKEEMRNFFTVLKQAVDTCKKCERTKETKAHQIINESKINSNTMSEDPIDKLVKLAKLLDDGLIDENEFANLKANVLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G4</accession>
    <name>F0T8G4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Helicase A859L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1481</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645066.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09715">
        <property type="protein sequence ID" value="ADZ09715"/>
        <property type="gene ID" value="Metbo_1481"/>
    </dbReference>
    <dbReference type="GeneID" id="10277932"/>
    <dbReference type="KEGG" id="mel:Metbo_1481"/>
    <dbReference type="eggNOG" id="arCOG12470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024787_2_1_2"/>
    <dbReference type="OMA" id="HALIFSE"/>
    <dbReference type="OrthoDB" id="68198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025280">
        <property type="entry name" value="DUF4041"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13250">
        <property type="entry name" value="DUF4041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10544">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00974">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="T5orf172" evidence="3">
        <location>
            <begin position="376"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="267"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="489" mass="57514" checksum="E62F291E43034750" modified="2011-05-03" version="1">MNIREDKGLILIIIGILLSITIIGIFFGIPLIIIGTYYLNKKVNQRENEINEELIAKKTELTNIDAILEEKEREKVKEIDEKLEGKKKELAYIDVKLEAMELQREKEMNAKMKEKKSQLVILDNELKEKKKGLILVDDELEMQEIGLYQPKYDFLTAILYKEKWDAIRKKQKALVKDKMAAICSTEWTIDNSKQKGKAFTNANLKQILKNFNLECDMIISKVKLSNRENSVKRINRAYDILNKLNERNRVNITPQYLDFKLQELDIAIEYELKKQEEKELLREAREQEKEERKIQKQLDAQEKKIKEQKKSLENDINTIGAELRESKSDEEKERLKLKIKELELALAKSNEDIDQIADRRKRTGAGYVYILSNIGSFGENVYKIGVTRRDEPQDRVRELSNASVPFKFDSHVFIFNKEAFELEKELHKRFDSKRVNKVNSRKEFFNITLDDVKKIVEENKELVHSFNVKPEAQEYYDTLKIDKITNTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K9</accession>
    <name>F0T8K9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-oxalocrotonate tautomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1530"/>
    <dbReference type="EnsemblBacteria" id="ADZ09760">
        <property type="protein sequence ID" value="ADZ09760"/>
        <property type="gene ID" value="Metbo_1530"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1530"/>
    <dbReference type="eggNOG" id="arCOG02240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_5_2_2"/>
    <dbReference type="OMA" id="KTHWGIG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="9043" checksum="EC4C8B82752C6F39" modified="2011-05-03" version="1">MCMVINVPVLNVNVWKGFEQAKIDYLIENLTKVFVDVGISAEAVEVIIHEVPQSHWGLGGVPCTKKFKDMDPDSWKKMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q8</accession>
    <name>F0T8Q8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methanogenesis marker protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0348</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0348"/>
    <dbReference type="EnsemblBacteria" id="ADZ08600">
        <property type="protein sequence ID" value="ADZ08600"/>
        <property type="gene ID" value="Metbo_0348"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0348"/>
    <dbReference type="eggNOG" id="arCOG00950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668380_0_0_2"/>
    <dbReference type="OMA" id="IHLGYTS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017672">
        <property type="entry name" value="MA_4551-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03278">
        <property type="entry name" value="methan_mark_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="19"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="423" mass="46368" checksum="53564E523D3DE1A3" modified="2011-05-03" version="1">MGKEVVNMQIIADVGGIPGKDCRGFCKYCYFRKVTGEDPLGCRSCTPGTVGCENCSTGVRETRNEFMHPFMVMGSVQNSLMMSEIRDNNLKANISGGGDVSCYPHLLELASGLNQYNLPIHLGYTSGKGFDSAKTAEELISLGVDEVTFTVFSTDQKLRDSWVKDPTKGESLNALKLFCENTEVHAASVIVPGVNDGDVLYETCSNLEDWGAKAFILMRFANFRNQGLILGNEPILKDVVPHTLVEFESLVRKINKEFNLRVTGTPLCDPENDTPFAISKEKNSEYLEILTDVQSEATVLSSNIAAPYIKKIVGMIGADELINVVPVDQDIGCLITQEDVEGVDLAELKETVIIPGRALIHDKVAQNILSKDGVDRIVARGPDKLSVDGEMSGTLSREDVLKKELIAFEDLIEAINFFGVRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R1</accession>
    <name>F0T8R1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyl-coenzyme M reductase operon protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0351</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 3">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643954.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0351"/>
    <dbReference type="EnsemblBacteria" id="ADZ08603">
        <property type="protein sequence ID" value="ADZ08603"/>
        <property type="gene ID" value="Metbo_0351"/>
    </dbReference>
    <dbReference type="GeneID" id="10276788"/>
    <dbReference type="KEGG" id="mel:Metbo_0351"/>
    <dbReference type="eggNOG" id="arCOG03225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099719_0_0_2"/>
    <dbReference type="OMA" id="PVCEITF"/>
    <dbReference type="OrthoDB" id="65557at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007687">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003137">
        <property type="entry name" value="McrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03264">
        <property type="entry name" value="met_CoM_red_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="21336" checksum="67FA77EAAC1A71ED" modified="2011-05-03" version="1">MIGKATHVVDCRETMGMGEGGGIAQRGTFAECGSEVLAIAMSPGRRHITKPVCEITFALREANIQTSTLVLNAGAGVPQDAPSAGAGSLFGLTPKEIEQIKRFRLVVVHLGGVRHHIIYKARLILRHVNRPCVVICEYPVDFEDFAKIGVKTRSVMPNEPNTKGEIVDIISGVIRGETCPQDKLDEIIRKVKLALGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U9</accession>
    <name>F0T8U9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645128.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09777">
        <property type="protein sequence ID" value="ADZ09777"/>
        <property type="gene ID" value="Metbo_1549"/>
    </dbReference>
    <dbReference type="GeneID" id="10278005"/>
    <dbReference type="KEGG" id="mel:Metbo_1549"/>
    <dbReference type="HOGENOM" id="CLU_2949307_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="7212" checksum="EC4229EE5736C436" modified="2011-05-03" version="1">MDYVPQLAEIGKKITEMNEFEVDAQEMDYISELYEYTKYMMMIYDGCDISRPQMMFIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T930</accession>
    <name>F0T930_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644003.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08652">
        <property type="protein sequence ID" value="ADZ08652"/>
        <property type="gene ID" value="Metbo_0400"/>
    </dbReference>
    <dbReference type="GeneID" id="10276837"/>
    <dbReference type="KEGG" id="mel:Metbo_0400"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551848_0_0_2"/>
    <dbReference type="OMA" id="TIAYICP"/>
    <dbReference type="OrthoDB" id="111975at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="172" mass="18669" checksum="62FFFE0DA41C80FF" modified="2011-05-03" version="1" precursor="true">MEKNQYLMPVIIVLVVIIALILALSVSGNNTFNTGTVSFQYPNSWSQDSLVGNFSNSSIYSSVTLTTNYADSSGKTQPAYIVLQMQAKATGSLNLPSTNNILLNTTNSSVASTTVNNITASQVGSVGNNIASKYTIIDKDNNYYLITYICPRFALNQTEQAYNNLLQTLKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T948</accession>
    <name>F0T948_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644021.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0418"/>
    <dbReference type="EnsemblBacteria" id="ADZ08670">
        <property type="protein sequence ID" value="ADZ08670"/>
        <property type="gene ID" value="Metbo_0418"/>
    </dbReference>
    <dbReference type="GeneID" id="10276855"/>
    <dbReference type="KEGG" id="mel:Metbo_0418"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155673_0_0_2"/>
    <dbReference type="OMA" id="DINYERV"/>
    <dbReference type="OrthoDB" id="113119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="109" mass="12135" checksum="DB24CF0BF12579DA" modified="2011-05-03" version="1">MVDLSSLYNLDVYTTRGNYVGRIQDVVLNIKKGRVSILKTTAMKPDKKSVGIKDVIKTSIRIVPEGDEIRPLKEEGSIEIPYERVQAVGDILLISPEIVKTPVETTPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T977</accession>
    <name>F0T977_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UbiA prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0447</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644050.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0447"/>
    <dbReference type="EnsemblBacteria" id="ADZ08699">
        <property type="protein sequence ID" value="ADZ08699"/>
        <property type="gene ID" value="Metbo_0447"/>
    </dbReference>
    <dbReference type="GeneID" id="10276885"/>
    <dbReference type="KEGG" id="mel:Metbo_0447"/>
    <dbReference type="eggNOG" id="arCOG00481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079545_0_0_2"/>
    <dbReference type="OMA" id="IFLKVYV"/>
    <dbReference type="OrthoDB" id="69690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="298" mass="34015" checksum="0DE6A71363C14D14" modified="2011-05-03" version="1">MILSIELKNHYSNFLSKIIQELVHGGYLTALGAPSLVLTTMIITNSEVNIPLLVISYLLPLIVYSFDYMRDLEKDLETNSERSKHLLKKKKFYPFLLAFYISILILLLLMFSGMNLVIFIGLITMGGLLYATALKSLTKKIPVFKNVYTVLTWSLGGTMFVSLFFSIPFSVPFLVVFIFINLKGMINAIFFDLKDYISDSKEGLKTLPVMLGRRNAINCLHILNVLAVIPLVLGVYYRIIPIISLSLIIFFFYSFYYLRTAQNADNDKVWVKLGSIADFEFMFWPLILISCMLVSNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C9</accession>
    <name>F0T9C9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1654</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645231.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1654"/>
    <dbReference type="EnsemblBacteria" id="ADZ09880">
        <property type="protein sequence ID" value="ADZ09880"/>
        <property type="gene ID" value="Metbo_1654"/>
    </dbReference>
    <dbReference type="GeneID" id="10278111"/>
    <dbReference type="KEGG" id="mel:Metbo_1654"/>
    <dbReference type="eggNOG" id="arCOG00027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019582_2_2_2"/>
    <dbReference type="OrthoDB" id="44488at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="PTHR43321"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="491" mass="55715" checksum="CDA3E018339FF604" modified="2011-05-03" version="1">MLSDKKNLEHMSKSEREHTTTYGSRYFRKTVPKYEMSEEGMPARAAYQIIHDELNLDGNPALNLASFVTTWMEPEADMLIMESIGKNYVDNDEYPQTEVIQNRVVNMLSRLFNAPKDAKSIGSGTIGSSESIMLGLLAHKWSWKKRREAEGKSTDKPNIVIGADVHTVWEKFALYFDVELKLIPLKKNICKETETEITQIMSSFSCISGLKEDVYTVTAEDIKKHVDENTIAVGAVVGTTFTGQMDPVEEINDALVEIKKTKGWDIPIHVDGASGGFVLPFLNPDLKWDFRLEQVRSINVSGHKYGLVYPGVGWLIFKDVSDLPEELIFKINYLGGIMPNYSLNFSKGSSTIIAQYYNLIRLGMKGYRDIMENMSENAHYLASKLNGSGKFDVINKSIQFPIVTVQLKNCDFTVFHLSEKLRQKGWIVPAYTLPEDADDVAVLRIVVKESFSKDMVEMLFDDIMESIDTLNQTTHERLQHIDEKENPTLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D4</accession>
    <name>F0T9D4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1659"/>
    <dbReference type="EnsemblBacteria" id="ADZ09885">
        <property type="protein sequence ID" value="ADZ09885"/>
        <property type="gene ID" value="Metbo_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1659"/>
    <dbReference type="eggNOG" id="arCOG10205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094458_0_0_2"/>
    <dbReference type="OMA" id="FSAPHCV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="SSF48537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn_dep_PLPC" evidence="1">
        <location>
            <begin position="28"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ada_Zn_binding" evidence="2">
        <location>
            <begin position="210"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="29291" checksum="99BF4A96E1D943BF" modified="2011-05-03" version="1" precursor="true">MNYYKLPMIFLAVCLIFAPVSDAWTWSTHSNIVDSTYYSLPTSIHSKLSLSAMRDGSNDPDEKFHDYRDHSYPNSYNKALYYLNQGSSYYKQKKYYEASKSFGIASHYISDTFSAPHCVSGELSSQHQAYENQASKLKPHITAVTGNLKVLMYNGYTTEKSDWSNWQKTRSTYYTQKDLNRAASVTYMAICNSIKSSSTTKPILTNTGKYVGNSKTKKFHLKNCRYVDQISPDHKVYFSTRQAAINAGYLPCKVCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I4</accession>
    <name>F0T9I4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphate ABC transporter, ATPase subunit</fullName>
            <ecNumber evidence="6">3.6.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.27" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644083.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0480"/>
    <dbReference type="EnsemblBacteria" id="ADZ08732">
        <property type="protein sequence ID" value="ADZ08732"/>
        <property type="gene ID" value="Metbo_0480"/>
    </dbReference>
    <dbReference type="GeneID" id="10276920"/>
    <dbReference type="KEGG" id="mel:Metbo_0480"/>
    <dbReference type="eggNOG" id="arCOG00231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="TIDICRV"/>
    <dbReference type="OrthoDB" id="78752at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="251" mass="28193" checksum="B6757353FC26F562" modified="2011-05-03" version="1">MPYRIEVKDLNVYFGESHILKDINININNNMVTALIGPSGCGKSTFIRTLNRMNDSISTFKKDGSVLLDGKDIYESKVDVVDLRKKVGMVFQKPNPFPKSIFDNVAYGLRIHGESDKDVLANKVEESLRAAALWDEVKDKLEMSALGLSGGQQQRLCIARTIAVEPEVILMDEPCSALDPISTTKIEDLIHKLKDDYTIIIVTHNMQQATRVSKYTAFFLNGEIVESGLTDKLFIEPEDKRTEDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L8</accession>
    <name>F0T9L8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PBS lyase HEAT domain protein repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0514</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644117.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0514"/>
    <dbReference type="EnsemblBacteria" id="ADZ08766">
        <property type="protein sequence ID" value="ADZ08766"/>
        <property type="gene ID" value="Metbo_0514"/>
    </dbReference>
    <dbReference type="GeneID" id="10276959"/>
    <dbReference type="KEGG" id="mel:Metbo_0514"/>
    <dbReference type="eggNOG" id="arCOG02966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_371590_0_0_2"/>
    <dbReference type="OrthoDB" id="105333at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="591"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="625"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="659"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="375"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="748" mass="87367" checksum="B7B73DB993EECB5A" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N0</accession>
    <name>F0T9N0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1683"/>
    <dbReference type="EnsemblBacteria" id="ADZ09909">
        <property type="protein sequence ID" value="ADZ09909"/>
        <property type="gene ID" value="Metbo_1683"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1683"/>
    <dbReference type="eggNOG" id="arCOG03177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_6_2"/>
    <dbReference type="OMA" id="DEMLCFS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="16380" checksum="AAF59C96A2B0EF0E" modified="2011-05-03" version="1">MVESEGPENYVCFKLNRVRRKIHRYYEEKLAQFKITPAQFYVLSALWDKDEIKFKDLAKRLDMDGATLTGVLDRMEKRDFIQRTEDTQDRRSVLVCLTSKSKDIRPQMIEIAKDLDAEFRKNVVEEDYNKLLAVLDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q2</accession>
    <name>F0T9Q2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645282.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09931">
        <property type="protein sequence ID" value="ADZ09931"/>
        <property type="gene ID" value="Metbo_1706"/>
    </dbReference>
    <dbReference type="GeneID" id="10278163"/>
    <dbReference type="KEGG" id="mel:Metbo_1706"/>
    <dbReference type="eggNOG" id="arCOG00520">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067890_1_2_2"/>
    <dbReference type="OMA" id="CHLGFVR"/>
    <dbReference type="OrthoDB" id="110669at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17691" checksum="5B2CF7AA14AA78F2" modified="2011-05-03" version="1">MRRSEKEIKDIKIIYKILNEADVCRVGFSKANKPYVVPMNFGFDESHIYLHSSVEGKKMEILKENKNICFEVDVKSEIVKSEKPCNWGMHYMSVMGFGTAELIEEPEEKIRALDIIMDKYDPEGLNSYEYLESSLKRTAVIKLNITELTAKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q5</accession>
    <name>F0T9Q5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Choloylglycine hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645285.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1710"/>
    <dbReference type="EnsemblBacteria" id="ADZ09934">
        <property type="protein sequence ID" value="ADZ09934"/>
        <property type="gene ID" value="Metbo_1710"/>
    </dbReference>
    <dbReference type="GeneID" id="10278167"/>
    <dbReference type="KEGG" id="mel:Metbo_1710"/>
    <dbReference type="eggNOG" id="arCOG10445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_625022_0_0_2"/>
    <dbReference type="OMA" id="TCANAIQ"/>
    <dbReference type="OrthoDB" id="11379at183925"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005079">
        <property type="entry name" value="Peptidase_C45"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03417">
        <property type="entry name" value="AAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="405" mass="45835" checksum="35440D0E8F0CAF98" modified="2011-05-03" version="1">MILKNEIVVPKADDEYLEVRKLVVKGSDFEIGKDLAKIGMEEYEVELDEYQSPVYGKARQNYMEKNFPALAERALGVADAFNLDPKNTKYDTTVLPFDLGDIACSAIYFPGSLTENKHPCICRNLDWYEGSVTELESALMGIKGKPARKSLSRITTVEFYPDNGYSAVQLGSHDLLNPPMDGVNEKGLFVTILTDLQGLKTPMPFGGAIDTGMSFPQLTTMILNKCATVEEAKFEILQQRIYFPFRAQHFLIGDKDGNVTLFEIDGNSGLYYFIDGIPDQPFIVTNHSMHLYPDPSKFPEYDPKEGYNTFNRWNRLDNYIKNHDGVFTKEDMFEAMSLVYSHYKDPMAAGGSFPFTERSMYTYVADIPEKSIEVKFYRKDGPALDGTDEPSIIFHDPVTLTLNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V9</accession>
    <name>F0T9V9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08782">
        <property type="protein sequence ID" value="ADZ08782"/>
        <property type="gene ID" value="Metbo_0530"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0530"/>
    <dbReference type="eggNOG" id="arCOG06499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149767_0_0_2"/>
    <dbReference type="OMA" id="FKATSMS"/>
    <dbReference type="OrthoDB" id="116188at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="15826" checksum="1DFC1985AB12FFF1" modified="2011-05-03" version="1" precursor="true">MVKKMKRSRLNLFKATSMSISVLGIILIIATLVLFAYIGISSLTSSITTDVGSGSAYDQLAVLKSDYNTLNDQFTSVQQSVDTSNNKNAKTAFINAKLELVKANSDINDVESALSAGKSKDEVTQRINAAQSQLNTARSSLASVKGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W0</accession>
    <name>F0T9W0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="2">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rlmE</name>
        <name evidence="5" type="ordered locus">Metbo_0531</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644134.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0531"/>
    <dbReference type="EnsemblBacteria" id="ADZ08783">
        <property type="protein sequence ID" value="ADZ08783"/>
        <property type="gene ID" value="Metbo_0531"/>
    </dbReference>
    <dbReference type="GeneID" id="10276976"/>
    <dbReference type="KEGG" id="mel:Metbo_0531"/>
    <dbReference type="eggNOG" id="arCOG00079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_2"/>
    <dbReference type="OMA" id="HRQTDHL"/>
    <dbReference type="OrthoDB" id="105478at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FtsJ" evidence="4">
        <location>
            <begin position="23"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="23057" checksum="3EB7D0A1B2C8311A" modified="2011-05-03" version="1">MGKKWNSERKTEHYYKMAKKQNYRSRASYKLQQLNKRFKVIKAADKVVDLGAAPGGWSQIALEAVGEEGTVVGVDLEWIRPLDEENFHTVRGDFTKDETLKEVKDLINGTAQVVISDAAPKLTGIKDIDTIRSTDLADNALTVCDHVLMQGGNFVLKVFQGVEYDNIIKNIKERFKVVKTTKPPSSRKASVEMYVVAKGFRRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y7</accession>
    <name>F0T9Y7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644161.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08810">
        <property type="protein sequence ID" value="ADZ08810"/>
        <property type="gene ID" value="Metbo_0558"/>
    </dbReference>
    <dbReference type="GeneID" id="10277003"/>
    <dbReference type="KEGG" id="mel:Metbo_0558"/>
    <dbReference type="eggNOG" id="arCOG13994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792153_0_0_2"/>
    <dbReference type="OMA" id="DWIEDIS"/>
    <dbReference type="OrthoDB" id="105458at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="139" mass="16769" checksum="77406AD7B2AB0EC7" modified="2011-05-03" version="1">MIGKDELVDKIETTKLERKQDLSDSYEKKIYLDRDLLLIVGYDLEENFENGDRDEETVRNFYWYWEIRNSKTWEVKFDNHDKDYSFCESTVGEWTDNDRVEDFVGDVAEEDVCKWSEQDWIEDISEDIADEVLGWLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA25</accession>
    <name>F0TA25_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="4" type="ordered locus">Metbo_0597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644199.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0597"/>
    <dbReference type="EnsemblBacteria" id="ADZ08848">
        <property type="protein sequence ID" value="ADZ08848"/>
        <property type="gene ID" value="Metbo_0597"/>
    </dbReference>
    <dbReference type="GeneID" id="10277042"/>
    <dbReference type="KEGG" id="mel:Metbo_0597"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_2"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="51806at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="2">
        <location>
            <begin position="11"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="3">
        <location>
            <begin position="362"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="469" mass="52350" checksum="9089004B420372B6" modified="2011-05-03" version="1">MNLRSGRLKGKMTADAVSFTSSLEFDKRIFNADIKCNIAHTTMLKEQGIIPSEDADKIVEALQALEVEGINALEWDMSVEDIHMALENYVTNRIGEVAGFMHTGKSRNDQVATDLRLVLKEEIEIIEEDILKFLDTLIEMASIHTETIMVGYTHLQHAQPTTFAHHLLAYANEVKRDYERLNDAKKRVDLCPLGSAAMTGTGFNIDRERTAELLGFGGVMENSMDGVSSRDFMAETVFALAMMASNLSKISEELIVWSSYEFRLVEISDEYSSTSSIMPQKKNPDVAEISRGKTAVLYGELMTIMSILKALPHSYNRDLQEITPHLWNAVDIGRSMLNITCSMLGSIEFNSERGLELALANFSTATELADLMVREKKLPFRIAHQIVGRTVTKAIDNHIKPDEIDAKFLDEVAIEINGEKLGLDQKTVEKALNPVEIIKARDVLGGPSPTAVKKSISSLKNFIELQRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA47</accession>
    <name>F0TA47_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09997">
        <property type="protein sequence ID" value="ADZ09997"/>
        <property type="gene ID" value="Metbo_1774"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1774"/>
    <dbReference type="HOGENOM" id="CLU_3075343_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="6366" checksum="A37C539C71FBB4E0" modified="2011-05-03" version="1">MNQINYVNDIALKLEPHETWRLMSYWPKIMERVHIIAKNMDMLLTEKSQQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA64</accession>
    <name>F0TA64_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645365.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1792"/>
    <dbReference type="EnsemblBacteria" id="ADZ10014">
        <property type="protein sequence ID" value="ADZ10014"/>
        <property type="gene ID" value="Metbo_1792"/>
    </dbReference>
    <dbReference type="GeneID" id="10278249"/>
    <dbReference type="KEGG" id="mel:Metbo_1792"/>
    <dbReference type="eggNOG" id="arCOG03950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207616_0_0_2"/>
    <dbReference type="OMA" id="PGHPQSK"/>
    <dbReference type="OrthoDB" id="50074at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="260" mass="28877" checksum="8D654FA91DDFE4BA" modified="2011-05-03" version="1">MKFSTVIIGAVALVLVFSFVITLFGPIQPLGRLSFVKLENPDMYPGHPHSVLLAKYAEEHNSKCALVVHFAGSSTYSSYLEDGVNSTTDPDGVYIIEVAFIDTQGGGSTSLNQTNFFDSLKVALFGVPDGRYKYMSDGVVYDNYDDMMKHVQKVAKQHGQDGPIPMVWHGTVRTDNPVIAPGCGFPLYFQILTKTYGIIPAYIYCITGLIFPYLESPYTNYELLHASELQYYYNNGYINIDYVKSGVNTAKYYGGTSSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA91</accession>
    <name>F0TA91_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nitrogenase iron protein</fullName>
            <ecNumber evidence="12">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nifH</name>
        <name evidence="15" type="ordered locus">Metbo_1819</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">The reversible ADP-ribosylation of Arg-101 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 14">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645392.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1819"/>
    <dbReference type="EnsemblBacteria" id="ADZ10041">
        <property type="protein sequence ID" value="ADZ10041"/>
        <property type="gene ID" value="Metbo_1819"/>
    </dbReference>
    <dbReference type="GeneID" id="10278276"/>
    <dbReference type="KEGG" id="mel:Metbo_1819"/>
    <dbReference type="eggNOG" id="arCOG00590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="TTMMDTL"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12 13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="12 13">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ10041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="30168" checksum="CA75C93D30E008AA" modified="2011-05-03" version="1">MVRKIAIYGKGGIGKSTTTQNTASAMAHFHGKKVMIHGCDPKADSTRMILRGKMQTTMMDTLREEGEEACMNLDNVMSTGFEGIKCVESGGPEPGVGCAGRGVITAITLMEQLKVYDDNDFVFFDVLGDVVCGGFAMPIRDGKAEEIYVVASGEMMALYAANNLCKGMVKYANQSGVRLGGIICNSRNVDGEKELLEEFCKKIGTQLIYFVPRDNMVQKAEFNKKTVVDFDAECNQAHEYEALAGKIINNENFVIPKPMSMDELEEMVLQYGLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB2</accession>
    <name>F0TAB2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Threonine synthase</fullName>
            <ecNumber evidence="5 9">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_0613</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644215.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0613"/>
    <dbReference type="EnsemblBacteria" id="ADZ08864">
        <property type="protein sequence ID" value="ADZ08864"/>
        <property type="gene ID" value="Metbo_0613"/>
    </dbReference>
    <dbReference type="GeneID" id="10277058"/>
    <dbReference type="KEGG" id="mel:Metbo_0613"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="OMA" id="MWGFQAS"/>
    <dbReference type="OrthoDB" id="16594at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="64"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="227"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="399" mass="42763" checksum="205C73A93C6FDE0A" modified="2011-05-03" version="1">MMKCISCGEEYEIDEIIYTCKKCDSTLEVVSSHPDISKDIFSCRKSTMWKYKEFLPVDETKIVSLEEGGTPFCKCDKLGSELGIDLYVKVEGSNPTGSFKDRGMSVGMTKAVELGVNTVGCASTGNTSASLAAYAARAGKRCIVLLPAGKVALGKLAQAMFHGAEVISIRGNFDEALETVTSLALEGQLYLLNSVNPYRLEGQKTIGFEIVDDLGWKSPDRIVLPVGNAGNISAIWKGIKEFHEAGFMDDLPMMTGIQAEMSAPITNAVRQGKNTIEPVKDPETIATAIRIGAPVSAQKALRAIYESNGYADTVTDDEILSAQKLLARKEGIGVEPASAASIAGLLKMVESGEVDKGEQVVCIVTGHLLKDPNTAIDACVEPLEIDADVKTLSKIINKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF3</accession>
    <name>F0TAF3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644256.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0654"/>
    <dbReference type="EnsemblBacteria" id="ADZ08905">
        <property type="protein sequence ID" value="ADZ08905"/>
        <property type="gene ID" value="Metbo_0654"/>
    </dbReference>
    <dbReference type="GeneID" id="10277099"/>
    <dbReference type="KEGG" id="mel:Metbo_0654"/>
    <dbReference type="eggNOG" id="arCOG02263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067830_0_0_2"/>
    <dbReference type="OMA" id="PEHSFYE"/>
    <dbReference type="OrthoDB" id="113556at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="13554" checksum="CED2288889DC3579" modified="2011-05-03" version="1">MKDILDTIKKNIGIDDENEHKEEQETIIVPEHSLYEIILMKAKNLDDFDFALSQITEEKNPIIMDMSTLERDSPEEFKLAGEKLKAFRDKTGGEAILLCKNGKNIIIITPPEIKLIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF6</accession>
    <name>F0TAF6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644259.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0657"/>
    <dbReference type="EnsemblBacteria" id="ADZ08908">
        <property type="protein sequence ID" value="ADZ08908"/>
        <property type="gene ID" value="Metbo_0657"/>
    </dbReference>
    <dbReference type="GeneID" id="10277102"/>
    <dbReference type="KEGG" id="mel:Metbo_0657"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_2_2"/>
    <dbReference type="OMA" id="INPNYIS"/>
    <dbReference type="OrthoDB" id="105856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="29027" checksum="4AC7A7F53F617D76" modified="2011-05-03" version="1">MSRFIAFASGKGGVGRTSITFNLGVALSLFGEEVVMLDLDLVMANLDVITGLLNPDVTLHDVLVRDKTIDECVYEVNQGARVVPTGIHFETLKHINPNYISWNKIMNEISDYGEVFLMDLPSGINANVFEGLPENTEAILVTQSTMPSVADALKIRILFNELNIDIKGFVLNMWYDDKFLLSENEIESILEIPLIGVIPYDREIERSMALGRSIVEVNPSSPTSNAVMQLAADLIGKEYKPIEPDKEGILGRIKKFVGLLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG1</accession>
    <name>F0TAG1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="2">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">DCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dcd</name>
        <name evidence="4" type="ordered locus">Metbo_0662</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dCTP + 2 H2O = diphosphate + dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644264.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0662"/>
    <dbReference type="EnsemblBacteria" id="ADZ08913">
        <property type="protein sequence ID" value="ADZ08913"/>
        <property type="gene ID" value="Metbo_0662"/>
    </dbReference>
    <dbReference type="GeneID" id="10277107"/>
    <dbReference type="KEGG" id="mel:Metbo_0662"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_2"/>
    <dbReference type="OMA" id="FENHRYP"/>
    <dbReference type="OrthoDB" id="91282at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="3">
        <location>
            <begin position="73"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="2">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="201" mass="22917" checksum="3150B8E4725DB3E3" modified="2011-05-03" version="1">MAILSDKDIKKHIQSGKIVIDPLKEPERQIQPSSVDLRIGNEFKGFKIIRKPCIDPMDESDIESYMESFYIDDGEPFIIHPGEFALATTYETIELPDNLVARVEGRSSMGRLGITMHVTAGYIDPGFRGKITLEISNIGKMPVALYTGQRVCQIVFETMTTPADRPYGHPERDSKYMNQERPVTSKIKHDYELVRRKNNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG2</accession>
    <name>F0TAG2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA methyltransferase, TrmH family, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644265.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0663"/>
    <dbReference type="EnsemblBacteria" id="ADZ08914">
        <property type="protein sequence ID" value="ADZ08914"/>
        <property type="gene ID" value="Metbo_0663"/>
    </dbReference>
    <dbReference type="GeneID" id="10277108"/>
    <dbReference type="KEGG" id="mel:Metbo_0663"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="OMA" id="PIMNLSH"/>
    <dbReference type="OrthoDB" id="93855at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="26029" checksum="308A0392500549C4" modified="2011-05-03" version="1">MIYVVFVEPESPGNIGFLARTMKNFGLTNLVLINPCELKNESYYQAMHARDVVSNCEIYETLEEFLEKANISTAVATTGNAGGSYNISKIAVTPETLSDSINYGGNICLIFGREGNGLSNQEIALCDVIVSIPTHEEYQIMNVTHAAAIIFYELFKKEKSYPVDTLEPASAQEKKSLTAIMDEIIVKLGYPEHKTKNAQLVFDRIIQRAFITGREAHTLKGTLRRINLRTKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG8</accession>
    <name>F0TAG8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenylacetate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644271.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0669"/>
    <dbReference type="EnsemblBacteria" id="ADZ08920">
        <property type="protein sequence ID" value="ADZ08920"/>
        <property type="gene ID" value="Metbo_0669"/>
    </dbReference>
    <dbReference type="GeneID" id="10277114"/>
    <dbReference type="KEGG" id="mel:Metbo_0669"/>
    <dbReference type="eggNOG" id="arCOG02620">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_1_2"/>
    <dbReference type="OMA" id="IQNTHGY"/>
    <dbReference type="OrthoDB" id="17209at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047475">
        <property type="term" value="F:phenylacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05913">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011880">
        <property type="entry name" value="PA_CoA_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006444">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="76"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C_2" evidence="2">
        <location>
            <begin position="334"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="49295" checksum="BA0E9CBCD3BE2B6E" modified="2011-05-03" version="1">MIWNEQVECMSDDERKKLQLEKLQAIVKYAYENVPYYRKRLDEIPLKPEDIKTLKDIEKIPFTTKDDLRDAYPFEMFAVPKKEIVEVHTSSGTTGKPTVSGYTRGDLEIWSEVMARGLTMAGVDDEDIIQNTHGYGLFTGGFGVHYGAQKIGATVIPISTGQTKRQIEIMQDFGTTVMIFTPSYGLYLAEEIKAEGLNPEDMDFKAIGFGAEMWTEEMRKEIEKRFNAPAYNIYGLTEIMGPGVGLECSAQDGLHIFEDHFYPEIIDPETFETVEDGEKGELVLTTLTREGTPLLRFRTKDVTKLTRSKCECGRTLVRMERVKGRTDDMMKIRGVSVFPSQIEKALLRIDGVEPHYQIIVTRPHLMDEMEVRVEASEDLFSDEIKELVGIREKIEQYIHNEIGLRVKVTLVEPKTIPRSEGKAVRVIDKRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH0</accession>
    <name>F0TAH0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644273.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0671"/>
    <dbReference type="EnsemblBacteria" id="ADZ08922">
        <property type="protein sequence ID" value="ADZ08922"/>
        <property type="gene ID" value="Metbo_0671"/>
    </dbReference>
    <dbReference type="GeneID" id="10277116"/>
    <dbReference type="KEGG" id="mel:Metbo_0671"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_940218_0_0_2"/>
    <dbReference type="OMA" id="SHTRWGW"/>
    <dbReference type="OrthoDB" id="61207at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="61"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="30981" checksum="87203056A3B0D9D8" modified="2011-05-03" version="1">MKIENYPLKRSSYESWETIFQNPAPIDVQTYQTGSVVIDLDGTFNPNHLRSPRISGTVEVPILSHFVTHEKLGNFLLDAGLDKSYYRDPYGGVETPQKEEYIQDKNQDILYQLGDNASELNAVFLSHMHPDHMAGMRDLPKKIPVILGKGELEDYEPDLYGDFLEDVKTVYEMDFTEFESISPFPKCADLLGDGSLWAISTPGHTRGHISFVVNGLAGPKLLTMDVAFIQENISYGVAPSDYTWNVEEAQRSLDMLINFIRLFRGMEVTAGHALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI1</accession>
    <name>F0TAI1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase S1 and S6 chymotrypsin/Hap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1831</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645404.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1831"/>
    <dbReference type="EnsemblBacteria" id="ADZ10053">
        <property type="protein sequence ID" value="ADZ10053"/>
        <property type="gene ID" value="Metbo_1831"/>
    </dbReference>
    <dbReference type="GeneID" id="10278288"/>
    <dbReference type="KEGG" id="mel:Metbo_1831"/>
    <dbReference type="eggNOG" id="arCOG02833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815379_0_0_2"/>
    <dbReference type="OMA" id="DINHYVE"/>
    <dbReference type="OrthoDB" id="88030at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="36946" checksum="1327694561AC2930" modified="2011-05-03" version="1" precursor="true">MNQNQRSVLLVVVVLVIMGFTAVIVFNSSSTSASTLNAQAADSVVYVENGVSGVVTITDPFLNKTSDVNIGYYPLDSGSGFVVNDEGYIVTAYHVVGDPQAVQDKGVLRLMESGDIQQYLERAAVSGYVSRYNPQLGLELLNNTTGQGLIQTQPDANTTTELMNQRNLLHVQSAKQQIRVRLPGSSVGQYVNANLVDVGNPETSEDMALIKINTFFIRLHPLTLNSNTPILNQRIHIYGYPATKQGSYSNFNSSSLKPNSSSGVVTNELPTNGTFYYETTAQTTYGFSGGPVLDSNNEVLGILIYKLVTSNNTDQSRSVFLSSQYITKLLTKNNVSVNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK3</accession>
    <name>F0TAK3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10075">
        <property type="protein sequence ID" value="ADZ10075"/>
        <property type="gene ID" value="Metbo_1853"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1853"/>
    <dbReference type="HOGENOM" id="CLU_1060117_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="30503" checksum="B9F7DC6339F932ED" modified="2011-05-03" version="1">MKKILAIIGFILIVCSCFIYIFTKSGDYILALILSVLIGTLVLVMVYTISVLKNASISNYYERVMIYLFIIAFIFVIILTTGLGMTWIPSYFNSVYLLLGSMMVAAIGFYLNNRLERDRESRKVKRIIYVVNDTIKTNQNHAENLLSNGWESKKTDTLNNGFWPIFMGNIIDFDIDPQLVRDLIEIKELTYNINEQIKGRNEYLRTLYVSITPEIDLFEGYEDTNQLKRLELLNNDLSTEVEKLIHDSKLYLDKYKEFMDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM8</accession>
    <name>F0TAM8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10100">
        <property type="protein sequence ID" value="ADZ10100"/>
        <property type="gene ID" value="Metbo_1879"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1879"/>
    <dbReference type="eggNOG" id="arCOG14011">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1084220_0_0_2"/>
    <dbReference type="OMA" id="WQGWACY"/>
    <dbReference type="OrthoDB" id="123041at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="268" mass="30908" checksum="8ABD1859750CA576" modified="2011-05-03" version="1">MMKMRKNMLYSSIISILIGLIWTMGNSYIGPLMLAFGIILTSFYLFNTKKPNSFAFIVVTLVTLALMFSLEYFMIHIQSTVFYVLLFVMSVGTVLTFFFSFKPMNVIDRRDKIIAWVGAIIFSISFFGLISIIIDNFEVSITFGALFLFLVVGMLFIRRKISKDIEEENEFEVENEPDEFYSTKLDFDEYWFKFKVGGIPKPISWQGWACYALILLSPGILIFVRDPTTCTIFVVAIIFIVTTVSILKSDYKATVKEYRENLKKENEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT8</accession>
    <name>F0TAT8_METLA</name>
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        <submittedName>
            <fullName evidence="2">Aspartate transaminase</fullName>
            <ecNumber evidence="2">2.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0713"/>
    <dbReference type="EnsemblBacteria" id="ADZ08964">
        <property type="protein sequence ID" value="ADZ08964"/>
        <property type="gene ID" value="Metbo_0713"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0713"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="29"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="372" mass="40518" checksum="CB1448907EE578D9" modified="2011-05-03" version="1">MVINMESSKRVSSINLSEIRRMFEIAGENAISLGLGEPDFKTPQNIIDAATQAMNEGFTHYTSNMGIIELREAIADKLEKENGISSSADSVIVTVGASEAVYVCMQALINPGDEVLIPDPGFLSYSACAKLADGVPVGIKIKEENDLRMTCDDVLESITPKTKAIILNSPSNPIGSVMKKKDVKGIAEIADDNDIYIISDEVYEKIIYDSKHYSAGQFSENAITINAFSKTYAMTGFRIGYISANDSIAEELLKVHQYIAACASSMAQKAALEALEGPQNSVKSMVSEFKKRRDLIIKRLSGMGIDCPVPEGAFYVFPKVDNPEKFVSDGLKKGVVMVNGKAFGEQGSDHVRLSYATAYDKLEEAMDRLESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU1</accession>
    <name>F0TAU1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0716"/>
    <dbReference type="EnsemblBacteria" id="ADZ08967">
        <property type="protein sequence ID" value="ADZ08967"/>
        <property type="gene ID" value="Metbo_0716"/>
    </dbReference>
    <dbReference type="GeneID" id="10277163"/>
    <dbReference type="KEGG" id="mel:Metbo_0716"/>
    <dbReference type="HOGENOM" id="CLU_2271066_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11827" checksum="77B6E0B1A3EF66CC" modified="2011-05-03" version="1">MATDDDNGKAKDLKLSDIIKKYDESNHQKLETSLKDYVGYFKKIYEELNMEWTAEDSLKVSAIFDAILERAKLDAIQILLSSTEGKEMDKPRKSEVHEKVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV4</accession>
    <name>F0TAV4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644331.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08980">
        <property type="protein sequence ID" value="ADZ08980"/>
        <property type="gene ID" value="Metbo_0729"/>
    </dbReference>
    <dbReference type="GeneID" id="10277176"/>
    <dbReference type="KEGG" id="mel:Metbo_0729"/>
    <dbReference type="eggNOG" id="arCOG00567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504010_0_0_2"/>
    <dbReference type="OMA" id="INGYWSP"/>
    <dbReference type="OrthoDB" id="69731at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="526" mass="61612" checksum="556116347B45C036" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV9</accession>
    <name>F0TAV9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase 28 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0734"/>
    <dbReference type="EnsemblBacteria" id="ADZ08985">
        <property type="protein sequence ID" value="ADZ08985"/>
        <property type="gene ID" value="Metbo_0734"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0734"/>
    <dbReference type="eggNOG" id="arCOG01393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060247_0_0_2"/>
    <dbReference type="OMA" id="FGHTTRC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_tran_28_C" evidence="1">
        <location>
            <begin position="211"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="378" mass="42396" checksum="A6C258F22D705906" modified="2011-05-03" version="1">MPCGIGMGHTSRCVTIAKELEKIGVEVAFASYGSGYKILSNHYNYELYKLPEIKFYGVECELDIKYTAKKSINVPFVFLKSIYKESRMIKKFKPDIIIADSHFSVPITAKVHGIPCVMIQNELTLNFAELYPDEKTMEYLESGLKKFVADVCNLSKVVMIPDVPGSIEIPTKLENKVVHTGPLLREDPRKMKDKQQLRSEMGFNSCDRIVLVTVGGSEFGLELLKLICLASSTIDADKIVVVSGPQINDDLILETEKIVKKKFLSNMMEWMKISDVIVTLAGHTTTMEVAALGIPNLTVPIEKHPEQLRNGSNIKKYGISIVEELKNLNIENISDNINFLLENTDIQSKVESVKTKFSSYTGTKNAVKIIMDNSRLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX7</accession>
    <name>F0TAX7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Putative cobalt transport protein CbiM</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Energy-coupling factor transporter probable substrate-capture protein CbiM</fullName>
            <shortName evidence="11">ECF transporter S component CbiM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cbiM</name>
        <name evidence="12" type="ordered locus">Metbo_0752</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CbiM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644354.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0752"/>
    <dbReference type="EnsemblBacteria" id="ADZ09003">
        <property type="protein sequence ID" value="ADZ09003"/>
        <property type="gene ID" value="Metbo_0752"/>
    </dbReference>
    <dbReference type="GeneID" id="10277200"/>
    <dbReference type="KEGG" id="mel:Metbo_0752"/>
    <dbReference type="eggNOG" id="arCOG02248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052508_3_0_2"/>
    <dbReference type="OMA" id="ANVFSMG"/>
    <dbReference type="OrthoDB" id="94360at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01462">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018024">
        <property type="entry name" value="CbiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627">
        <property type="entry name" value="PTHR43627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00123">
        <property type="entry name" value="cbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="2 11" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="231" mass="24768" checksum="67E6DC5BD1D28011" modified="2011-05-03" version="1">MHIAEGFLPWQWCLFWYIVALPVIAYGIIQIKKVTDENPESKPLLAVSGAFIFILSSLKMPSVTGSCSHPTGTGLGAVLFGPAAVSVLAAIVLVFQALLLAHGGITTLGANDFSMGIVGPFAAWLIWKGARKIGWSTSIAIFLAAVMGDWLTYVTTAVQLSLAFPIPTFEAAFVKFIGIYAWTQVPLAIAEGILTVVVFEYIMKLRPDIMVRLGVIKSEDPEIVKAKAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB32</accession>
    <name>F0TB32_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1958</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1958"/>
    <dbReference type="EnsemblBacteria" id="ADZ10178">
        <property type="protein sequence ID" value="ADZ10178"/>
        <property type="gene ID" value="Metbo_1958"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1958"/>
    <dbReference type="eggNOG" id="arCOG02487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02516">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_225877_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025666">
        <property type="entry name" value="PQQ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="PQQ_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKD"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="542"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="626"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="935"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="1274"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="2178"/>
            <end position="2256"/>
        </location>
    </feature>
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        <location>
            <begin position="2261"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="4">
        <location>
            <begin position="2344"/>
            <end position="2424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="2864" mass="303452" checksum="D3BB70CD3AAA41E4" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB96</accession>
    <name>F0TB96_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl30</name>
        <name evidence="4" type="ordered locus">Metbo_0796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0796"/>
    <dbReference type="EnsemblBacteria" id="ADZ09047">
        <property type="protein sequence ID" value="ADZ09047"/>
        <property type="gene ID" value="Metbo_0796"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0796"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="OMA" id="KDYITWG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_L30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_L30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_L30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_L7/L30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_L7_euk_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="PTHR11524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L30" evidence="3">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="159" mass="17806" checksum="BD3111219F3435A3" modified="2011-05-03" version="1">MRIILGDKMIAALRVRGRTGVKRDIQETMSMLNLTRINHMVLIHTNPSYDGMLKKAKDYITWGDVDAETLSAIIAKRGRLPGDAKVTDEYVKDNTDYSSIEDLAKAIVEGEAKVEDAGIKPVFRLHPPRKGYESTKKSFKEGGSLGKRENINELVTKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB99</accession>
    <name>F0TB99_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Adenylate kinase</fullName>
            <shortName evidence="8">AK</shortName>
            <ecNumber evidence="3 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adkA</name>
        <name evidence="9" type="ordered locus">Metbo_0799</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644401.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0799"/>
    <dbReference type="EnsemblBacteria" id="ADZ09050">
        <property type="protein sequence ID" value="ADZ09050"/>
        <property type="gene ID" value="Metbo_0799"/>
    </dbReference>
    <dbReference type="GeneID" id="10277247"/>
    <dbReference type="KEGG" id="mel:Metbo_0799"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OMA" id="HRDEMRK"/>
    <dbReference type="OrthoDB" id="79684at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20783" checksum="24497F3C9C761AFF" modified="2011-05-03" version="1">MKLVVLAGIPGSGSTTVLTKSLEQLDFVHVNYGDVMLEIAKAKNLVEDRDSLRKLSPELQKEVQREAAQTIRAKSEQSNIIVDTHCTINTPSGFLPGLPKWVLEQLMPNMFVLLEADGDEILKRRISDTTRARDSENLKDINLHQEMNRAASMAYAVLTGATVKIIENHDNKLDESVEEMVKTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD0</accession>
    <name>F0TBD0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644432.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0830"/>
    <dbReference type="EnsemblBacteria" id="ADZ09081">
        <property type="protein sequence ID" value="ADZ09081"/>
        <property type="gene ID" value="Metbo_0830"/>
    </dbReference>
    <dbReference type="GeneID" id="10277279"/>
    <dbReference type="KEGG" id="mel:Metbo_0830"/>
    <dbReference type="eggNOG" id="arCOG06456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029703_1_0_2"/>
    <dbReference type="OMA" id="EKFEVPF"/>
    <dbReference type="OrthoDB" id="13627at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="167"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="50495" checksum="3524343FBD021D7E" modified="2011-05-03" version="1">MDEVSEYYHDSRMKKMFQLLEEPKSMVEIDISEAFIKNLVLKIISTYGTIVVNQIHEITGLHVDILEEVLKKLDKEGMCGQTGGGFLFPSVEFTIKIKGREKALQVMQENPYVGIAPVAYDEYFTIMGAQVQGRFPLKIPKSVIDNALDDVVGVADAKNTLIASAIGGKGFFIYGAPGTGKTFLTSKMSDLLPPILIPKFIEFSGSVIQLYDPDFHKKCPEQPDDPRWVKVYAPFVFTGSELTTEKLETNFNPNKGVYETSPIIKANGGILLLDDLGRQKEDHNVLLNRMIVPLENKKDVIYIKGAPVIVHTMFIPVLSTNLDINIVDEAHLRRAPMHIFLTAPTPDEIVEVFQRNLDAMGEDYDEAVLERFKNVYIPMTMGGEQLKPTFAHARDVAQIAQAVRIRNNQEKLDTEVLEEALDEHILIALQRKYTPDLFERIINKKVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE9</accession>
    <name>F0TBE9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645569.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10218">
        <property type="protein sequence ID" value="ADZ10218"/>
        <property type="gene ID" value="Metbo_1999"/>
    </dbReference>
    <dbReference type="GeneID" id="10278459"/>
    <dbReference type="KEGG" id="mel:Metbo_1999"/>
    <dbReference type="HOGENOM" id="CLU_1458207_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="19700" checksum="0F5E2A423CCBC032" modified="2011-05-03" version="1" precursor="true">MIKKCGILMLSLLVLFVVPANAVQIGSQEESFNLNSDNEIKNIEIKDMLLNNNETADINGTVTNETTDLNSTVGNYTNETFVNSTVGNDTNSTVGNDTNSTVDKEKLKEQKQKLKENIVNTLNNVGVISGVVGGVLMTMSSILITVPDPTWITKVITAGCIVGTAAAAAVSIGCAISSILVQWFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ8</accession>
    <name>F0TBJ8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="8">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpE</name>
        <name evidence="12" type="ordered locus">Metbo_2048</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645618.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2048"/>
    <dbReference type="EnsemblBacteria" id="ADZ10267">
        <property type="protein sequence ID" value="ADZ10267"/>
        <property type="gene ID" value="Metbo_2048"/>
    </dbReference>
    <dbReference type="GeneID" id="10278510"/>
    <dbReference type="KEGG" id="mel:Metbo_2048"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
    <dbReference type="OMA" id="HGRMDTS"/>
    <dbReference type="OrthoDB" id="13784at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="11">
        <location>
            <begin position="22"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="10">
        <location>
            <begin position="188"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="52094" checksum="C5F0D428CD2AABB2" modified="2011-05-03" version="1">MNVFGEYNIKIKQPKSIDLNSDSPFELFKNLYTNHSSSFLLESMESDSGMARFSVLGFEPAAILRARENVLEVDIDGHTEEIDVKNPFDEIRKLTRPNNGKKGFCGGLVGYVSYEAARHFLPIDLKPGNKPDYEFGLFLDGIVYDRIRNTCKYVTLGENRFEEVEKFSKQEIPTGELSYQETNHYFTKKEFESMILDVKERIKAGEIFQDVISNAREYELSGNKLSFYENLRRINPSPYMYHLKLGENEIIGSSPEMLVRVENRMIESFPIAGTRKRGKTDVEDEKLEIELLEDEKERAEHLMLVDLARNDVGKVSEFGSVTVPEYMGVKKFSHVQHIVSHVTGKLRKDKDAVDAFEAMFPAGTLSGAPKIRAMEIIDELEQVPRGPYAGAVGYFALNGNADFAITIRTMVCHDNHATVQAGAGIVYDSVAQEEYYETENKAGALLTALRSGNNLENTPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM1</accession>
    <name>F0TBM1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intimin/invasin family.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ09098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09098">
        <property type="protein sequence ID" value="ADZ09098"/>
        <property type="gene ID" value="Metbo_0848"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0848"/>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262271_0_0_2"/>
    <dbReference type="OMA" id="TITWIIG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003344">
        <property type="entry name" value="Big_1_dom"/>
    </dbReference>
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        <property type="entry name" value="CarboxyPept-like_regulatory"/>
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        <property type="entry name" value="DUF11"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Invasin_dom_3"/>
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        <property type="entry name" value="Thioester_dom"/>
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        <property type="entry name" value="DUF11"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Invasin_D3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF49373"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51127">
        <property type="entry name" value="BIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big-1" evidence="2">
        <location>
            <begin position="316"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1295" mass="136683" checksum="2DAE042FA8E0FBAE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM2</accession>
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        <submittedName>
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        </submittedName>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="Metbo_0849"/>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10764" checksum="EDF3EA443A152874" modified="2011-05-03" version="1">MNKIYDISSLVVADDVVSCNLDDEVAILNTQSGIYYGLDPIGAKIWNSLQKPCSISDLIELILEEYDVEEERFKTDLLDLVNDLKEKGLVKVNVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM8</accession>
    <name>F0TBM8_METLA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Metbo_0855</name>
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    <organism evidence="1 2">
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="ADZ09105">
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    <dbReference type="HOGENOM" id="CLU_3322949_0_0_2"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="38" mass="4542" checksum="B1D84C67A2BABA3A" modified="2011-05-03" version="1">MYIIFKIGIILANKRVKYQRIFVPIKQDIIFGNFVLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP4</accession>
    <name>F0TBP4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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    <organism evidence="1 2">
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="gene ID" value="Metbo_0872"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0872"/>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="SSF55724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="178" mass="19797" checksum="7C0C6F42A45DB13A" modified="2011-05-03" version="1" precursor="true">MNKKTIVPIVFLIVALVAVSGCTSNNGNSTSVKNYTGNGVSFSYPGNWTLENQTTGNDTIMVTDLSSMNASRVTTSFEVQIIPLNGLTEQSALAQFNNTIHPTEWKKISNNTLTINDKTAYQRTYIANDTNYNQTMRYSEILFVKNGNIYIITLQAQDNTFDSVKSKFDTIINSLKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR9</accession>
    <name>F0TBR9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Undecaprenyl-phosphate glucose phosphotransferase</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0897</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.8.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644497.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0897"/>
    <dbReference type="EnsemblBacteria" id="ADZ09146">
        <property type="protein sequence ID" value="ADZ09146"/>
        <property type="gene ID" value="Metbo_0897"/>
    </dbReference>
    <dbReference type="GeneID" id="10277346"/>
    <dbReference type="KEGG" id="mel:Metbo_0897"/>
    <dbReference type="eggNOG" id="arCOG06282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_1_2"/>
    <dbReference type="OMA" id="NWSVLFD"/>
    <dbReference type="OrthoDB" id="38630at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:undecaprenyl-phosphate galactose phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
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        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017473">
        <property type="entry name" value="Undecaprenyl-P_gluc_Ptfrase"/>
    </dbReference>
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        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WcaJ_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="7">
        <location>
            <begin position="280"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="463" mass="53815" checksum="816384FF16B665DF" modified="2011-05-03" version="1">MIRENQRTLTTLQVLFDVVAMTLALYLAWLIRVKTDILGPGFDVWTFPQYAASLAFIIVSYTILSYFFNLYNSQRTDKLGNEVKQILKVNIIGLLLLITALYIIEVADYSRYMLAMFAVFSTIFMCIERIIVKNALMYVRGKGYNVQYILVIGAGELGESFARKIKQNFYLGYEIIGFLDDNIAKGQKVDGSKIIGSINDLEQIILTKTIDLAVITISARHYQVIENTVNTLEKCGVKAEIVPDFYRYFPAKPYIDMIDDIPVINIRYVPLDDNLNKILKRMSDIILVTIGIIIISPILIVTAILVKLTSPGPVIFKQKRVGCNRKEFNMYKFRSMRVQEEEQFKWTQKDDPRKTRIGSFIRKTNIDELPQFFNILKGDMSLIGPRPERPYFVDKFRDEIPKYMVKHHVRPGMTGYAQINGYRGDTSIKKRIDYDIYYVENWNLYLDVKIFFMTFRSFFKNAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV2</accession>
    <name>F0TBV2_METLA</name>
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        <recommendedName>
            <fullName evidence="4">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_2075</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645645.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2075"/>
    <dbReference type="EnsemblBacteria" id="ADZ10294">
        <property type="protein sequence ID" value="ADZ10294"/>
        <property type="gene ID" value="Metbo_2075"/>
    </dbReference>
    <dbReference type="GeneID" id="10278537"/>
    <dbReference type="KEGG" id="mel:Metbo_2075"/>
    <dbReference type="eggNOG" id="arCOG01158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454660_0_0_2"/>
    <dbReference type="OMA" id="EFRRMAS"/>
    <dbReference type="OrthoDB" id="21534at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="210"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="224"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="281"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="600" mass="68268" checksum="90B5E1A53B58C2CA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV8</accession>
    <name>F0TBV8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645651.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10300">
        <property type="protein sequence ID" value="ADZ10300"/>
        <property type="gene ID" value="Metbo_2081"/>
    </dbReference>
    <dbReference type="GeneID" id="10278543"/>
    <dbReference type="KEGG" id="mel:Metbo_2081"/>
    <dbReference type="eggNOG" id="arCOG03597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157711_1_0_2"/>
    <dbReference type="OMA" id="HEICKCG"/>
    <dbReference type="OrthoDB" id="118506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020075">
        <property type="entry name" value="Uncharacterised_AF2234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10967">
        <property type="entry name" value="DUF2769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="11677" checksum="CDBB529A2F6ADDE4" modified="2011-05-03" version="1">MGKVDLNRENINKCLCATCPVQVDSKCSKDKLELLNQKLEKEGDVRDIFKDEELPLVYCAFGVAECGDLSSTELCKCTQCPVWLENNLANNEPIEYFCVEGVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY0</accession>
    <name>F0TBY0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Creatininase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645673.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2103"/>
    <dbReference type="EnsemblBacteria" id="ADZ10322">
        <property type="protein sequence ID" value="ADZ10322"/>
        <property type="gene ID" value="Metbo_2103"/>
    </dbReference>
    <dbReference type="GeneID" id="10278566"/>
    <dbReference type="KEGG" id="mel:Metbo_2103"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192640_0_0_2"/>
    <dbReference type="OMA" id="FLIINCH"/>
    <dbReference type="OrthoDB" id="84020at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="PTHR35005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="SSF102215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="235" mass="25364" checksum="6D4E1CF0D2372EB5" modified="2011-05-03" version="1">MTDLRYEAGNVVSPQVHSVGVLAVGSHLENHGAALPIDTDSKIAAYIGLQAALITGAKYLGILYSATEYEYVEHGIHQEPEELVNKQLIPTLRSAKKLLQLDSVVLVNGHGGNVPVNDYLEHVQLETGLKIIFNNKIVEIEGPHAGTGEVSVGELLGILDKTKLDEHCNFENYPEVGMVGFDKARKAEEGINQGALEVIDTGVCIDLELGESILQTAIVAVMDDVEKLTADTKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ2</accession>
    <name>F0TBZ2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="5">FGAR amidotransferase III</shortName>
            <shortName evidence="5">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purS</name>
        <name evidence="6" type="ordered locus">Metbo_2115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645685.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2115"/>
    <dbReference type="EnsemblBacteria" id="ADZ10334">
        <property type="protein sequence ID" value="ADZ10334"/>
        <property type="gene ID" value="Metbo_2115"/>
    </dbReference>
    <dbReference type="GeneID" id="10278578"/>
    <dbReference type="KEGG" id="mel:Metbo_2115"/>
    <dbReference type="eggNOG" id="arCOG04462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_3_0_2"/>
    <dbReference type="OMA" id="PVIHDYE"/>
    <dbReference type="OrthoDB" id="117509at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9543" checksum="9C3BC8E86445EA74" modified="2011-05-03" version="1">MKYDAEVRISLKKGMLNPEAATIQRALALLEYQVENTSTVDLIRFQIQGDSEGAVKEEVDEMCQRLLCNPVIHDYTIKISAQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC46</accession>
    <name>F0TC46_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="10">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="10">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pheT</name>
        <name evidence="12" type="ordered locus">Metbo_0950</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644548.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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        <property type="entry name" value="B3/B4_tRNA-bd"/>
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        <property type="entry name" value="DNA-bd_dom_put_sf"/>
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        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
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    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="11">
        <location>
            <begin position="273"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="553" mass="62538" checksum="9979EFB36EE5E385" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC64</accession>
    <name>F0TC64_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644566.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0968"/>
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        <property type="protein sequence ID" value="ADZ09215"/>
        <property type="gene ID" value="Metbo_0968"/>
    </dbReference>
    <dbReference type="GeneID" id="10277417"/>
    <dbReference type="KEGG" id="mel:Metbo_0968"/>
    <dbReference type="eggNOG" id="arCOG02573">
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    </dbReference>
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    <dbReference type="OMA" id="MHIHGMI"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="20044" checksum="96D69EDA93A7CF64" modified="2011-05-03" version="1">MVKIIGIVGSPRTDGNTAFLVETALKSAEEVGAETEIINLGSASLEPCVACDICKATGECAIYDDMGEIMEKIMEADGMIIGSPVYFGSVTSQLKMFIDRSRPLRGSFRLRNKVGGALAVGASRNGGQESTIATIHDFLLIHDAIIVGDGAPMAHYGGTGVGGPKGSTEQDDVGIATSQNLGKRVAELAALIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC73</accession>
    <name>F0TC73_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase</fullName>
            <ecNumber evidence="2">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.98.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644575.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0977"/>
    <dbReference type="EnsemblBacteria" id="ADZ09224">
        <property type="protein sequence ID" value="ADZ09224"/>
        <property type="gene ID" value="Metbo_0977"/>
    </dbReference>
    <dbReference type="GeneID" id="10277426"/>
    <dbReference type="KEGG" id="mel:Metbo_0977"/>
    <dbReference type="eggNOG" id="arCOG00333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092359_0_0_2"/>
    <dbReference type="OMA" id="TEMPACE"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="122"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="26517" checksum="D8D86A86D7969B12" modified="2011-05-03" version="1">MLYFKGCVVREKLPGVAQATEKILKKAGVDYTLLGNESCCGSFLMRTGFKDEAEEVMKNNLQDILAANEKTILVSCSGCYNTLKNDYKALFDVELDVIHTSELVKELLVKNKIHVNSTEKTVTYHDPCHLGRHSGIYEEPRDAIKASADLVEMKRNRERSRCCGAGSGVKSAFPELALEVGCRRIQDAEEVGADILVTTCSFCILNLENALEKTKNQGENGSVERVMDLTELILMGMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA8</accession>
    <name>F0TCA8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645726.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2160"/>
    <dbReference type="EnsemblBacteria" id="ADZ10375">
        <property type="protein sequence ID" value="ADZ10375"/>
        <property type="gene ID" value="Metbo_2160"/>
    </dbReference>
    <dbReference type="GeneID" id="10278623"/>
    <dbReference type="KEGG" id="mel:Metbo_2160"/>
    <dbReference type="HOGENOM" id="CLU_803186_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="345" mass="40882" checksum="C92792B877F47073" modified="2011-05-03" version="1">MIDQVGQMDEIFDRIDREAEEFLEIERQKEERVRLEKERILNSMSPEKRQIVECVDKAMNKQNTMFCKLSNEQFVKEHCIYKLCKERNIQHERPSSRMNNNTEDSINIVYGFAFELLDEIEVQSWNVELIITDLYRYDPKKSQPAIEKIRRVPNHRAVNPLLKALKKSKKHHELIYSALKFYIKEQDIDLNLYSDKDIVEWSAKYALINLCLQPYTKNHRVSELVEAVPEFAKFIAKVGKQLNSADPQLRIVGVKHIGADKEFLEILKRISYEDPDEKVRDATRVSLRRNHKIKVQSVLNDPYTEYSPDEDTNTESYSEINRRDFEAQIEENEEYMDDIIAGLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE2</accession>
    <name>F0TCE2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber evidence="4 10">3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mch</name>
        <name evidence="11" type="ordered locus">Metbo_2195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible interconversion of 5-formyl-H(4)MPT to methenyl-H(4)MPT(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the MCH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.27" evidence="4 10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645760.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2195"/>
    <dbReference type="EnsemblBacteria" id="ADZ10409">
        <property type="protein sequence ID" value="ADZ10409"/>
        <property type="gene ID" value="Metbo_2195"/>
    </dbReference>
    <dbReference type="GeneID" id="10278658"/>
    <dbReference type="KEGG" id="mel:Metbo_2195"/>
    <dbReference type="eggNOG" id="arCOG02675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876031_0_0_2"/>
    <dbReference type="OMA" id="DKGMFAP"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00545">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02289">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03120">
        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="7 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="320" mass="33993" checksum="403F6F51D8407839" modified="2011-05-03" version="1">MVSVNLEAKKTVDYMIENADDIGIAVSKLENGSTILDCGVNVSGSFKAGELYTKICLGGLAEVGISIPGDLSENFAIPSVKVKTNFPAISTLGAQKAGWSVSVGDFFALGSGPARALSLKPAETYEEIDYKDDADIAILTLEADKLPGEDVADNIASACGVKSEDVYLLVAPTSSLVGSIQIAGRVVENGTYKMLEFLKFDVKKVKHAAGIAPIAPVDPDGLKAMGKTNDAVLFGGRTYYYVESEEGDDIKALAEQLPSSSSEGYGKPFFDVFKEAEFDFYKIDKGMFAPAEVVINDMRTGETFKAGGVNVELLKKSFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF3</accession>
    <name>F0TCF3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Archaeal Lon protease</fullName>
            <ecNumber evidence="13">3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Metbo_2206</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645771.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2206"/>
    <dbReference type="MEROPS" id="S16.005"/>
    <dbReference type="EnsemblBacteria" id="ADZ10420">
        <property type="protein sequence ID" value="ADZ10420"/>
        <property type="gene ID" value="Metbo_2206"/>
    </dbReference>
    <dbReference type="GeneID" id="10278670"/>
    <dbReference type="KEGG" id="mel:Metbo_2206"/>
    <dbReference type="eggNOG" id="arCOG02160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="OMA" id="GLYFWNL"/>
    <dbReference type="OrthoDB" id="7657at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
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            <begin position="127"/>
            <end position="144"/>
        </location>
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            <begin position="428"/>
            <end position="607"/>
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        <location>
            <position position="514"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="557"/>
        </location>
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        </source>
    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG5</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        </location>
        <ligand>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09242.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH7</accession>
    <name>F0TCH7_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09254">
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        <property type="gene ID" value="Metbo_1007"/>
    </dbReference>
    <dbReference type="GeneID" id="10277456"/>
    <dbReference type="KEGG" id="mel:Metbo_1007"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="11252" checksum="737B94E6A6980B73" modified="2011-05-03" version="1">MINCPAVIIGFIIAIVLSIIGGMFGLFGSGFGVLIAGAIVGFMVNGDILNGTMHGTLIGIVGALILALLAILFGHVSTIIALYVGVATVGSVLLSIVMGAIGGALGSLLSRWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ7</accession>
    <name>F0TCQ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <reference evidence="2" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645798.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2233"/>
    <dbReference type="EnsemblBacteria" id="ADZ10447">
        <property type="protein sequence ID" value="ADZ10447"/>
        <property type="gene ID" value="Metbo_2233"/>
    </dbReference>
    <dbReference type="GeneID" id="10278699"/>
    <dbReference type="KEGG" id="mel:Metbo_2233"/>
    <dbReference type="eggNOG" id="arCOG09721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152345_0_0_2"/>
    <dbReference type="OMA" id="FFNVMEL"/>
    <dbReference type="OrthoDB" id="113125at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16100" checksum="3504DA0E44F84789" modified="2011-05-03" version="1">MSETPIIKIKSNPETIKIIAKKRGDVSIQDINLRLIMANLWWEQAPELETFFNVMELTIKRALNEVYPHDVMTIDYTYSADDDLKDASEIVVEITNIKADDMDVDIGGRFITIGGSDSRGFFKKLTSFRRKFSQDVHKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR1</accession>
    <name>F0TCR1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
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                <person name="Lapidus A."/>
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                <person name="Pitluck S."/>
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                <person name="Land M."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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                <person name="Brauer S.L."/>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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                <person name="Lapidus A."/>
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                <person name="Pitluck S."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ADZ10518">
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        <property type="gene ID" value="Metbo_2304"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
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        <source>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
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                <person name="Liu W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="EMBL" id="ADZ10521.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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    <accession>F0T655</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645913.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2349"/>
    <dbReference type="EnsemblBacteria" id="ADZ10562">
        <property type="protein sequence ID" value="ADZ10562"/>
        <property type="gene ID" value="Metbo_2349"/>
    </dbReference>
    <dbReference type="GeneID" id="10278815"/>
    <dbReference type="KEGG" id="mel:Metbo_2349"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_0_2"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="OrthoDB" id="52310at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="292" mass="32189" checksum="A74E4AED6F88F216" modified="2011-05-03" version="1">MFLHTENVLKFAFSNETNDPYETSESLKFGILGVPFDGTTSYLPGARFGPRTVREASYNFEAYNFQLKKTVPTGVLDLGDIEVVHGNFKKTCSKLKSTIDDVLSQNIIPISIGGDHSISFCIINAITQALGENKLTVIHFDAHMDLRDEYGGEKYSHATVMRRILDLNPLQLIQIGVRSAAEEEATLSDERLTSFSSHQVCEDIENIINCLKNISGPIYISFDIDVLDPAYAPSTGTPSPCGLDPMQIEKLILNLESKELVGMDLVEVASTEIGDSTAMNAAKIIFDFLCLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T673</accession>
    <name>F0T673_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UPF0060 membrane protein ynfA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2367</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645931.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2367"/>
    <dbReference type="EnsemblBacteria" id="ADZ10580">
        <property type="protein sequence ID" value="ADZ10580"/>
        <property type="gene ID" value="Metbo_2367"/>
    </dbReference>
    <dbReference type="GeneID" id="10278833"/>
    <dbReference type="KEGG" id="mel:Metbo_2367"/>
    <dbReference type="eggNOG" id="arCOG07820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117653_2_0_2"/>
    <dbReference type="OMA" id="WLWAVEG"/>
    <dbReference type="OrthoDB" id="102851at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00010">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003844">
        <property type="entry name" value="UPF0060"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36116">
        <property type="entry name" value="PTHR36116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02694">
        <property type="entry name" value="UPF0060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="109" mass="12393" checksum="49726E951F849946" modified="2011-05-03" version="1">MEYLKSIFYFVVAGLFEIGGGYLIWLWIREGRGIEFAILGAVILVLYGVIPTLQPANFGRVYAAYGGIFIFMALLWGWQVDKITPDRFEIIGATIALFGVFIIMYWPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A1</accession>
    <name>F0T6A1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ordered locus">Metbo_1172</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644767.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1172"/>
    <dbReference type="EnsemblBacteria" id="ADZ09416">
        <property type="protein sequence ID" value="ADZ09416"/>
        <property type="gene ID" value="Metbo_1172"/>
    </dbReference>
    <dbReference type="GeneID" id="10277622"/>
    <dbReference type="KEGG" id="mel:Metbo_1172"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OMA" id="FRCNLIT"/>
    <dbReference type="OrthoDB" id="17268at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02535">
        <property type="entry name" value="hyp_Hser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="401" mass="44028" checksum="7897B73C66FF0482" modified="2011-05-03" version="1">MKYIVVIGDGMADYPLKELGGKTPLQGANTPNMDKIALNGVSGLLKTVPDNMTPGSDVANLSIFGYNPEKYYTGRGPIEAASIGAKMDEQDVAFRCNLITEKSGLLSDFNADHISTVEASEIIETLNTKFHDVGKFYLGTSYRHLFVTKKPNSANLISTPPHDVVNEPINNNLLKPSTDASAIELNNVMEESKKILENHPVNQKRMEEGKNPANMIWLWGQGVKPALPNFKDKYGVKAATITGVDLIKGLGVYMGLTNIEVPGATGYFDTDYCAKAKNTVDALEDHDMVFVHVEAPDEAGHAGDLEEKIKAIERIDDRIIGKLLDELPSFKEYSIAVLPDHPTPISIRTHTRDPVPFSMFSSNGESDTVNQYDEESVKNGVMGISWGYEFIEKFLNGSKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C9</accession>
    <name>F0T6C9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644795.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1201"/>
    <dbReference type="EnsemblBacteria" id="ADZ09444">
        <property type="protein sequence ID" value="ADZ09444"/>
        <property type="gene ID" value="Metbo_1201"/>
    </dbReference>
    <dbReference type="GeneID" id="10277651"/>
    <dbReference type="KEGG" id="mel:Metbo_1201"/>
    <dbReference type="eggNOG" id="arCOG04806">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131496_11_0_2"/>
    <dbReference type="OMA" id="FVMLEQW"/>
    <dbReference type="OrthoDB" id="118444at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="11150" checksum="8EA928CFB489D0EA" modified="2011-05-03" version="1">MIVVTAKMNVKPASKYDFMVETEALIKHTRYEKGCISYNLYTDTDDPNQLVMLEFWKDMDDLDAHMNTVHFKAFGNAISKYLTCEIEISKFDAQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D5</accession>
    <name>F0T6D5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heterodisulfide reductase subunit C, HdrC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644801.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1207"/>
    <dbReference type="EnsemblBacteria" id="ADZ09450">
        <property type="protein sequence ID" value="ADZ09450"/>
        <property type="gene ID" value="Metbo_1207"/>
    </dbReference>
    <dbReference type="GeneID" id="10277657"/>
    <dbReference type="KEGG" id="mel:Metbo_1207"/>
    <dbReference type="eggNOG" id="arCOG00965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100474_0_0_2"/>
    <dbReference type="OMA" id="CPRNNSP"/>
    <dbReference type="OrthoDB" id="102216at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="196" mass="21851" checksum="F15BAEBD2114CC8E" modified="2011-05-03" version="1">MRTIKLNKNSHKLVDDVLKDLKASPDLGIYKCVQCGMCTSICPGASQSDYDPRDMVRRVLEDDVSIVDDENIWNCFSCYTCNSVCPSGNNASEVNQILRQMSIDKGEGILKIQSFAAYGDSFIELGVGSVPSKFFDDMVKDVGPEYMNLKLNIEDIRSDLGLGSYILPEKSIDEVEEILDKSGFKARLKRIKGCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G6</accession>
    <name>F0T6G6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645951.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2387"/>
    <dbReference type="EnsemblBacteria" id="ADZ10600">
        <property type="protein sequence ID" value="ADZ10600"/>
        <property type="gene ID" value="Metbo_2387"/>
    </dbReference>
    <dbReference type="GeneID" id="10278853"/>
    <dbReference type="KEGG" id="mel:Metbo_2387"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029016_0_0_2"/>
    <dbReference type="OrthoDB" id="131094at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="31196" checksum="7FD18EBE47DB4328" modified="2011-05-03" version="1">MRIWEILYESAKYPFYGVKQFILLGFIILITVSVFGYYGLFGNYLHHFLGVDYWVLLLLLISLAFILLLFEAGYAFRMVQKSIEGINVPPKFNKFDIMFKHGINETVLISIYFSIPLIILYYILNYTLTPMPIIILFRLVDYLTSGWNILISPIPDQMLFILVIFVVILGFILDMIFTVAIPYMASKGGSFREAFNFKEIFKKIKKIGLRRMLIAYFLVLLTIVVMGGPLLIKEISQLNIIGFIIAEGLIAPYIVMLDSRFIALLYMNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J2</accession>
    <name>F0T6J2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_2413</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645977.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2413"/>
    <dbReference type="EnsemblBacteria" id="ADZ10626">
        <property type="protein sequence ID" value="ADZ10626"/>
        <property type="gene ID" value="Metbo_2413"/>
    </dbReference>
    <dbReference type="GeneID" id="10278885"/>
    <dbReference type="KEGG" id="mel:Metbo_2413"/>
    <dbReference type="eggNOG" id="arCOG00598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_1_1_2"/>
    <dbReference type="OMA" id="SWDNRGT"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005973">
        <property type="entry name" value="NifE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01283">
        <property type="entry name" value="nifE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="7">
        <location>
            <begin position="41"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="455" mass="50054" checksum="851BBD4C8B18555D" modified="2011-05-03" version="1">MEPVIETFESRKSHMCVKGDGISIPVCDKASLPGTVTQRTCVYGGARIVLMPITDSIHLVHGPIGCAACTWDIRGSKSSREDLYKKGCSTDLQETDIVFGGEKKLFETVIELNKLYHPGAIFVYATCVAGIIGDDIKAVCKKAEEITGCRVIPVQSEGYQNHNKTKGHWIGGDALLDYVIGTKEPEKTTPFDINIVGEFNVAGDLWGIKPLIEAMGINIISTLTGDSHVDEIAQAHRAKLNIVQCQKSSNYVAKKMEERYGIPFIKVNFFGLEQTVNSLRDIADFFGDPEMIERTEEIIANGLKEVEHKTDEYKKRLKGKTVALYVGGNKAWSLVRAFEELGMDVMMSGTKNGIKEDYERIKETVKDGTIIVDDANSTELARLLKKYKPNLLISGAKEKYISLKLGIPFCDFNHDRISAFAGFRGFVSFAKEVDASVSSPVFNLSSELGGGLNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L5</accession>
    <name>F0T6L5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="3">2.7.8.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">EPPG:FO PEP transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cofD</name>
        <name evidence="4" type="ordered locus">Metbo_2436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + H(+) + oxidized coenzyme F420-0</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CofD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646000.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2436"/>
    <dbReference type="EnsemblBacteria" id="ADZ10649">
        <property type="protein sequence ID" value="ADZ10649"/>
        <property type="gene ID" value="Metbo_2436"/>
    </dbReference>
    <dbReference type="GeneID" id="10278908"/>
    <dbReference type="KEGG" id="mel:Metbo_2436"/>
    <dbReference type="eggNOG" id="arCOG04395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055795_1_0_2"/>
    <dbReference type="OMA" id="DLDTVMY"/>
    <dbReference type="OrthoDB" id="31831at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="PTHR43007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="301" mass="33372" checksum="BBF735EBF75E9775" modified="2011-05-03" version="1">MITVLSGGTGTPKLLEGLVKVSDPKNLNIIVNTLENDYFSGVYVAADIDTVMYTLAGIINTETWYGIENDTFITHETLKEINCPETLRIGDKDRATKIQKTMLMEKYPLSTVVNIQKNALGIKSEIKPMSNDKSNITIVTDQGEMKFHEFLIEKQGKPHVKDIFYNQVEPSPGLIESIEESEMVILGPSNPITSLWPIISMKHVVKTLEKAYVVGISPIIGNSPVSGPAAKFMNALGHDVSCFGVAKMYSKFLDKFIIDLVDVDYKDQIERLIPEAVVTNTNMKNIGDKMMLAKISLGEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q2</accession>
    <name>F0T6Q2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643636.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0032"/>
    <dbReference type="EnsemblBacteria" id="ADZ08285">
        <property type="protein sequence ID" value="ADZ08285"/>
        <property type="gene ID" value="Metbo_0032"/>
    </dbReference>
    <dbReference type="GeneID" id="10276454"/>
    <dbReference type="KEGG" id="mel:Metbo_0032"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038046_4_2_2"/>
    <dbReference type="OMA" id="RDPLAWQ"/>
    <dbReference type="OrthoDB" id="64461at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="19"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="405" mass="43937" checksum="D35BD4A58B51F2B0" modified="2011-05-03" version="1">MHKLSTNFDKNSLKLPWKTLSIAIVLNFFIWAYLFITPAMETVLAANLHISHFQTSLLFTAPILMIALVAIPAGITADRIGLKKTMGIGAIVACIGAVLRGTAPDYSSLLLFSVIFGIGMGLTFANLPKLARSCSSVEQTSSVMGFVNGFGVLAGIGLALAITVPIIYSFTNSYQDVFYIWALPLILTTILWWIIVDEPPCTDHEPESDTGSMGLIEVLKNKTLWLLAIILLLHNIFFYTWSGWIPTYLLEKGISMNVSGLLTSIMLWVGIPTVILVPALYSRINVSKKLLILVPSLIFTFLALWIMPAAGFSILIIMIVAGIINILRFNTLLSLPVEIMPKKHSGVAGGVVISIGYLGAVIGPSMAGQILDITGSFQPIFIILAVLSLITMGLTFLLPKNKQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U8</accession>
    <name>F0T6U8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PAS sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644819.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09468">
        <property type="protein sequence ID" value="ADZ09468"/>
        <property type="gene ID" value="Metbo_1226"/>
    </dbReference>
    <dbReference type="GeneID" id="10277677"/>
    <dbReference type="KEGG" id="mel:Metbo_1226"/>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149818_0_0_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="195"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="27657" checksum="0B7CC5BE416053E7" modified="2011-05-03" version="1">MKIKNNLDELKSILKNSPDSIFVHNLKFDLIYANKGSCESQLYNNNKFGTSHFNELDQLIHYNLQKNYNDLINNGSTYFESSKICYDGSTIDVDVDSNIMESEEDSIILSIARNSCEIESPNTDQKELYEMLKTTRKGMIIDNLGIITYLNHNLCNQLGYSKDEILGHEYIDFFKGVQEFSILDELVKVKMGGVSNLISKMERKDGSNLWMLTTISPIYIKNEEYLGNLSTLRDITELVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y0</accession>
    <name>F0T6Y0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutathione synthase</fullName>
            <ecNumber evidence="6">6.3.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.3" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644851.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1258"/>
    <dbReference type="EnsemblBacteria" id="ADZ09500">
        <property type="protein sequence ID" value="ADZ09500"/>
        <property type="gene ID" value="Metbo_1258"/>
    </dbReference>
    <dbReference type="GeneID" id="10277709"/>
    <dbReference type="KEGG" id="mel:Metbo_1258"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053807_0_0_2"/>
    <dbReference type="OMA" id="KTEYYQV"/>
    <dbReference type="OrthoDB" id="44016at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040657">
        <property type="entry name" value="GshAB_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18419">
        <property type="entry name" value="ATP-grasp_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="71"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="37600" checksum="CEFA29C9E1AB2504" modified="2011-05-03" version="1">MQREYEQLELSTQIIINEAQKRGVEVEVLDWDENFIRLIKGDRVEYIKQGTKTSLDNYVVPLIMENKELTKLILREHDINVPDSVTVKNLECSMEIYSDFENKDIVIKPKSANFGEGVLIIKDLKSKEVYANAIEDAFNYSEDVLIEDYVAGEEYRFLVVTDEVLAVMYRVPANVKGDGINNIQELVDEKNQDPRRGKGYITPLEKIEIGPIERHNLNAQNIDVNYVPKKGEVVYLRENSNLSTGGDSIDFTDEVHEDYKQIAIKSAEAVEAKICGVDIIIPDVKAKPDKNNHSIIELNFNPVIYPHNFPYIGQNRHVEKKILDLLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z4</accession>
    <name>F0T6Z4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formylmethanofuran--tetrahydromethanopterin N-formyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.101</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.101" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644865.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1272"/>
    <dbReference type="EnsemblBacteria" id="ADZ09514">
        <property type="protein sequence ID" value="ADZ09514"/>
        <property type="gene ID" value="Metbo_1272"/>
    </dbReference>
    <dbReference type="GeneID" id="10277723"/>
    <dbReference type="KEGG" id="mel:Metbo_1272"/>
    <dbReference type="eggNOG" id="arCOG02695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="OMA" id="CGDGYEW"/>
    <dbReference type="OrthoDB" id="40013at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FTR" evidence="3">
        <location>
            <begin position="10"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="FTR_C" evidence="4">
        <location>
            <begin position="151"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="33661" checksum="39666BF8FDA6BFED" modified="2011-05-03" version="1">MKTHENLESKVVNTYCEAFKGICSRVIVTADDEETLKRAAYDATSTPGTVIGRVEGGIESWLNEDQTPDNRKGAILQFWYNTSDIEKFGVELSYRIRQDILVKPFTSLFDASQNPDGWIGTMKNVGHCGDGYEWEETIYNREMIVVPIAIPDFKIEKQIGYMNGIMGANFWYMCTDKKTVLEAGRTALKAIESVEGVIAPFDICSAASKPETNYPWIGPTTNHPYCPSLKNQLKESTMVPPEVKYIPEIVINGLNQTCLKEAMKLGIESLIDYDGVFKVSAGNYDGKLGEYKIDLKELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T703</accession>
    <name>F0T703_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Respiratory-chain NADH dehydrogenase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644874.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1281"/>
    <dbReference type="EnsemblBacteria" id="ADZ09523">
        <property type="protein sequence ID" value="ADZ09523"/>
        <property type="gene ID" value="Metbo_1281"/>
    </dbReference>
    <dbReference type="GeneID" id="10277732"/>
    <dbReference type="KEGG" id="mel:Metbo_1281"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="OMA" id="KIMARIQ"/>
    <dbReference type="OrthoDB" id="88886at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="31295" checksum="D6FEF49D39619E10" modified="2011-05-03" version="1">MNLMTNILINVIIAFLIGSLLFGLQRKIMARIQGRPGPPIIQHILHTLKFFIKESAFPYTAAMPFYIAITAMLSMIWVAAVIVGPVTGGSLLLIFGIYAVHKIVEHNAGSSSGSPYGKLSCVRAVYSAAAEIPLFAVLVIIYFKTGTMNITGLINYQAVHGPLLYAIPLAALMFFVLIMSKAPYSPFAITKGKDIVSGYETEHFGLLRGYLMMSESIAWYMLLWVFLTVFLGPISIPVYLIGMIVMSSIIAFINATTPLLNPNHSIMMQVTFAFIGIVGSFALLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A5</accession>
    <name>F0T7A5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643685.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0081"/>
    <dbReference type="EnsemblBacteria" id="ADZ08334">
        <property type="protein sequence ID" value="ADZ08334"/>
        <property type="gene ID" value="Metbo_0081"/>
    </dbReference>
    <dbReference type="GeneID" id="10276504"/>
    <dbReference type="KEGG" id="mel:Metbo_0081"/>
    <dbReference type="eggNOG" id="arCOG01396">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039259_0_0_2"/>
    <dbReference type="OMA" id="IYNPIEM"/>
    <dbReference type="OrthoDB" id="62518at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="356" mass="39160" checksum="BC0F7C7F00B05E9F" modified="2011-05-03" version="1">MKIAVLAEMAPAKAFVPIIQQLDADVIGLTHGHGVEEILGNYCTEIHSIGQSRGHGAKKRSNSKIATLVMEDVWRVIKFLRGRDIDLLMTCGNAGDVRKGISAAKILRIPNLHIEQDIYNPIEMLAFSNLVTVPSPEYKEYVEARYGLKNVHSIGGYPMAVYVDSLEMEDSDSVKTKYSVDEFYLLVLGGDVKGEDVPNIIRQVESLNTNILVVPFRFSESYVKSFIKSPNLKVLEGYVELPGLMEASKGIIYGAGMGLTIEAGVLSTPAVKIAGFHRKHASVDLAHEIGIEVAEIKDIADAVLEIKTPNGRKLVDDGKTAVKNTLEIIQNFDKLKTTSSGIQSFRSIWNARSEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F8</accession>
    <name>F0T7F8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Type III restriction protein res subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643738.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0134"/>
    <dbReference type="EnsemblBacteria" id="ADZ08387">
        <property type="protein sequence ID" value="ADZ08387"/>
        <property type="gene ID" value="Metbo_0134"/>
    </dbReference>
    <dbReference type="GeneID" id="10276560"/>
    <dbReference type="KEGG" id="mel:Metbo_0134"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033880_0_0_2"/>
    <dbReference type="OMA" id="WAEMYAT"/>
    <dbReference type="OrthoDB" id="7855at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="DEAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="50"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="579" mass="67374" checksum="5E3F08CE339050C9" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7G1</accession>
    <name>F0T7G1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flavodoxin/nitric oxide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643741.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0137"/>
    <dbReference type="EnsemblBacteria" id="ADZ08390">
        <property type="protein sequence ID" value="ADZ08390"/>
        <property type="gene ID" value="Metbo_0137"/>
    </dbReference>
    <dbReference type="GeneID" id="10276563"/>
    <dbReference type="KEGG" id="mel:Metbo_0137"/>
    <dbReference type="eggNOG" id="arCOG00509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="OMA" id="PEAPIYC"/>
    <dbReference type="OrthoDB" id="72715at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="260"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="45318" checksum="CD2D692C656B2190" modified="2011-05-03" version="1">MKSEAVKMKEGVYWVGFLDWDLRTYHGYTLNGTTYNAYLVFGKEKVALIDNTYHGKSYQMWGRIEDAFKKEGRDFKIDVIVQNHVEKDHSGALTEIHHKFPEAPIYCTEVAVEGLINHYPSLKEANFITVKTGDALDLGGATLAFLEAPLLHWPDSMFTLLAEEGILFSNDAFGQHICLPQRYDNEIPEYVLMDATKKFYANLITPLSKLVLKKFEEVTELGLLEKITMIAPAHGQIWTDPMKVIGAYSEWATGNVENKVTIVYDTMHGSTQKMAHAVAEGAISQGADVKIYYLHEDERSEIVKDILDSKAVAFGIPTIYDEPFPSVGDIVYYLRGLKFDRTGLKRKAATFGSMGGKGGAPKILKDDLENCGFDVKDEFEIYYLPTEDELERCFEIGKELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I4</accession>
    <name>F0T7I4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644903.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1310"/>
    <dbReference type="EnsemblBacteria" id="ADZ09552">
        <property type="protein sequence ID" value="ADZ09552"/>
        <property type="gene ID" value="Metbo_1310"/>
    </dbReference>
    <dbReference type="GeneID" id="10277761"/>
    <dbReference type="KEGG" id="mel:Metbo_1310"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_3_2_2"/>
    <dbReference type="OMA" id="WNTDESR"/>
    <dbReference type="OrthoDB" id="105754at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13925" checksum="AFE059388C70013E" modified="2011-05-03" version="1">MKQVFEKFEIEMRRGVMQLAVICLLDKEKYGYEIIKNLNDTGLKVEEGTLYPLLRRLEGNELLISRWETGGPRPRKYYIITDLGKEIRLKWLSTFKALMDTITQLETNIQDKGVSKNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N1</accession>
    <name>F0T7N1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644950.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1359"/>
    <dbReference type="EnsemblBacteria" id="ADZ09599">
        <property type="protein sequence ID" value="ADZ09599"/>
        <property type="gene ID" value="Metbo_1359"/>
    </dbReference>
    <dbReference type="GeneID" id="10277810"/>
    <dbReference type="KEGG" id="mel:Metbo_1359"/>
    <dbReference type="eggNOG" id="arCOG06074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896062_0_0_2"/>
    <dbReference type="OMA" id="KSEIYGC"/>
    <dbReference type="OrthoDB" id="57665at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="36651" checksum="E6A7F1D050477134" modified="2011-05-03" version="1">MTGYTVKLIEPQAKDDMFDELVSKVRYERKANINGACVKLLTDNHDFKEEWEDNFKFMNEDIRPHAKIFTVDDGGELEVLYETVSKTCIIKNCDYYGWVKSIALAVISDFFEEYHSVHRRYSVHGSTVDYEGHAIALIGPPGTGKSTLTYGLLQNKDFNYISDDWFFTRLFENATVVYASEKNSYIRDDIINVWTCFTDELKRVKLDNKGRGLADVNTLFKGRSRESSTLMSVVLLERDTNHPPFKKLELEEAMKFMEEKDFCNPHQLVKDNRKFKLRYNFFREVFSKLDVYLLNIIETPEQSLERIKKLAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P4</accession>
    <name>F0T7P4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644963.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09612">
        <property type="protein sequence ID" value="ADZ09612"/>
        <property type="gene ID" value="Metbo_1372"/>
    </dbReference>
    <dbReference type="GeneID" id="10277823"/>
    <dbReference type="KEGG" id="mel:Metbo_1372"/>
    <dbReference type="eggNOG" id="arCOG10295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077332_0_1_2"/>
    <dbReference type="OMA" id="FTIMAEI"/>
    <dbReference type="OrthoDB" id="110028at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032580">
        <property type="entry name" value="SatD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16264">
        <property type="entry name" value="SatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="26251" checksum="F1BC1F52C67EE276" modified="2011-05-03" version="1">MKEIDESHSVPRNNEFYVLLGDVVSSRKIENREKFQNHLINTYSELNQIYETDLYAKLDIIKGSDEIGCVLHRIDHLYEIISSITNKLNPHYMRFVCVKGIVDAGISTKKISQMDGPVFHKASDLMDSLKKEELIFRMNTENKVIDSLVTNNINLIYLIKSKWSLKKFKIINEYERSKDQVSTAEKLNLTPQVISYNLKSANWSELHRIETDLKESLKLIARYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R0</accession>
    <name>F0T7R0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
            <ecNumber evidence="2">3.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.47" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646093.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2529"/>
    <dbReference type="EnsemblBacteria" id="ADZ10742">
        <property type="protein sequence ID" value="ADZ10742"/>
        <property type="gene ID" value="Metbo_2529"/>
    </dbReference>
    <dbReference type="GeneID" id="10279003"/>
    <dbReference type="KEGG" id="mel:Metbo_2529"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_2"/>
    <dbReference type="OMA" id="RAHACGH"/>
    <dbReference type="OrthoDB" id="32735at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050118">
        <property type="term" value="F:N-acetyldiaminopimelate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="201"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="417" mass="45470" checksum="58E02F6703AA06AA" modified="2011-05-03" version="1">MGKESFQDLVNQLTIEHAPEQIKQFQWLHQHPELAYQEFESGRYIASYLETLDGLEVIYPFAKTGIKAVLKGENSKNAVAIRADFDALPVKEDTGLSYASRSKGTYNGQETYVSHVCGHDASAATAMGTATVLNQLKDDLNGDVVFLFQPAEEGVPDGMKAGADLMVSEGALKNPDVSAIFALHPYSKAYPGTVLLSKNTTHAGLNDLVIKIKGVQAHGSMPWVGRDPIVAGAAIINSLQTIVSREADLMRGAAVVTVGYFHGGIKVNIIPDHAEMGLTIRALDENNLKLLVKRVTEITKLVSKAHGCEAEIIMGQHDPMNKNDPDLCKNMLKTIRQVAGDGNMILKPATTGSEDFSYFSNKVPGLYLHFGTAPQNKPLSESRPNHHPGFMVDDDALKFATKLECCLLHNYLKQRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R2</accession>
    <name>F0T7R2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaerobic ribonucleoside-triphosphate reductase</fullName>
            <ecNumber evidence="6">1.17.4.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.4.2" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646095.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2531"/>
    <dbReference type="EnsemblBacteria" id="ADZ10744">
        <property type="protein sequence ID" value="ADZ10744"/>
        <property type="gene ID" value="Metbo_2531"/>
    </dbReference>
    <dbReference type="GeneID" id="10279005"/>
    <dbReference type="KEGG" id="mel:Metbo_2531"/>
    <dbReference type="eggNOG" id="arCOG01967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002707_0_0_2"/>
    <dbReference type="OMA" id="YSYDRVP"/>
    <dbReference type="OrthoDB" id="5218at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="5">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="773" mass="87453" checksum="0A12F74A9D804DC8" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V6</accession>
    <name>F0T7V6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmA</name>
        <name evidence="6" type="synonym">ksgA</name>
        <name evidence="9" type="ordered locus">Metbo_0191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643794.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0191"/>
    <dbReference type="EnsemblBacteria" id="ADZ08443">
        <property type="protein sequence ID" value="ADZ08443"/>
        <property type="gene ID" value="Metbo_0191"/>
    </dbReference>
    <dbReference type="GeneID" id="10276617"/>
    <dbReference type="KEGG" id="mel:Metbo_0191"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_2_2"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="99734at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="30"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="31056" checksum="C14266EBB0FEC7B9" modified="2011-05-03" version="1">MLSETLKILKQNGIKLDKRKGQNYLVNRDILSKIIQNSELSKNDRVLEIGAGIGTLTIPLAKNAGKVYAVEQDGKAANVLKKRLEKLELDNVEVIVEDATKMDLPSVNKVISNLPYQISSPITFKILENPFEMAVLMYQKEFAQRMVASPGDRNYSRLSVMMYLYSEAEILFQVSENDFFPKPKVASAVIKMQPKIDVDIDPFFVDVTRAMFQHKKKKIRNALIDSYHEIGDVEKSDLKTIISQLDQTKMNERVFKMSPEDLLKISGDIKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W6</accession>
    <name>F0T7W6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein Metbo_0201</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643804.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0201"/>
    <dbReference type="EnsemblBacteria" id="ADZ08453">
        <property type="protein sequence ID" value="ADZ08453"/>
        <property type="gene ID" value="Metbo_0201"/>
    </dbReference>
    <dbReference type="GeneID" id="10276627"/>
    <dbReference type="KEGG" id="mel:Metbo_0201"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_1_0_2"/>
    <dbReference type="OMA" id="LRLAHMI"/>
    <dbReference type="OrthoDB" id="103162at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="DUF99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="PTHR39518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="DUF99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="202" mass="22462" checksum="C57F5C83E8E809F5" modified="2011-05-03" version="1">MKNRTEIKKFRSIKQEIRILGVDDSPFPTHTKEEVMIVGTVFRAGNWLDGVLSTHVLGDGTDATRKISEMVLNSRNIGQLGVIMLDGITFAGFNVVNIRELYEATGVPVIVIMRKIPSFEGIKKALKRFPDWKDRWANVVEAGSVHRVDDKESIYIQFHGIKIEDAMEIVKLSITRSSIPEPIRVAHIIAAGIVTGESHGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y6</accession>
    <name>F0T7Y6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase, subunit beta</fullName>
            <ecNumber evidence="5">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643824.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0221"/>
    <dbReference type="EnsemblBacteria" id="ADZ08473">
        <property type="protein sequence ID" value="ADZ08473"/>
        <property type="gene ID" value="Metbo_0221"/>
    </dbReference>
    <dbReference type="GeneID" id="10276649"/>
    <dbReference type="KEGG" id="mel:Metbo_0221"/>
    <dbReference type="eggNOG" id="arCOG02651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OMA" id="REYGCEK"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="86"/>
        </location>
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        <location>
            <begin position="95"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="294" mass="31966" checksum="60FF9C32C7CE7D2D" modified="2011-05-03" version="1">MVLGTYKEVVTARSTDKEIQKIAQDGGIVSALFCYALEEKLIDGAVVAGPGKDFWKPEPMVAQTADEILAAAGTKYTFSPNVLMLKKAVRQYGLEKVGTVAIPCQAMGIRKMQSYPFGVRFLADKIALLTGIFCMENFPFASLQTFISEKMGVSPELVEKMDIGKGKFWVHTADDVLSIPLKETHGYEQSGCKVCLDYVAELADVSTGSVGSPDGWSTVFTRTDAGETVFKAAVEAGVIETKPVDTGKFGLEILTKLATQKKEKAMKEIDRRKEMGLPVPFKASSEKEDPLANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0T800</accession>
    <name>F0T800_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_1386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09626">
        <property type="protein sequence ID" value="ADZ09626"/>
        <property type="gene ID" value="Metbo_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1386"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850648_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="16335" checksum="EA05253D35B3DC04" modified="2011-05-03" version="1">MRCKNCGVENEDDAEYCRYCSCGLNESSSSDSTRDKMQNQFKKMYYIYFTAITFLIVPFFIPIIYNYFFHTPLNPIVVMLNLILLITGSLFTIYFYILFYKKKDELRGQEPYYSQITNIAILLFLLMITILVNYVTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T804</accession>
    <name>F0T804_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644981.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1390"/>
    <dbReference type="EnsemblBacteria" id="ADZ09630">
        <property type="protein sequence ID" value="ADZ09630"/>
        <property type="gene ID" value="Metbo_1390"/>
    </dbReference>
    <dbReference type="GeneID" id="24964554"/>
    <dbReference type="KEGG" id="mel:Metbo_1390"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041771_3_0_2"/>
    <dbReference type="OMA" id="CIFIGLE"/>
    <dbReference type="OrthoDB" id="79655at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="38318" checksum="8A76B5E0046FF893" modified="2011-05-03" version="1" precursor="true">MNNFKLQSISILLVLLLFFSLLSFGILFQILSLILFGAMIAYIVRHLAGKLHPFVRFETLAIFLAMIILAVPIVLVAYFTIHQLFLVAVDITGALPAPNTNTTMINQTMVSNDVQSAGPINGVAANLLDEIANLVAQSVVWLVGQIISFVASLPALFTDVIVLLFSVFYFAKEGDKFVGFIEDLLPKTPTFSEIYSEVDAILKSIMITNILCAVILGLLSVVLYYILGYPYILLLGILTAFSEFVPVVGPWIVYGALGVVDILTGNPVRGVIVIVVGWLIDTVVDMYIRPKLAGEFTNVHPLIFLVGFLFGALTLGLPGLFIGPLIVGVTYVLFEKYREERLKINQQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T847</accession>
    <name>F0T847_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645024.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09673">
        <property type="protein sequence ID" value="ADZ09673"/>
        <property type="gene ID" value="Metbo_1435"/>
    </dbReference>
    <dbReference type="GeneID" id="10277886"/>
    <dbReference type="KEGG" id="mel:Metbo_1435"/>
    <dbReference type="eggNOG" id="arCOG09752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119855_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="14491" checksum="58C60D7D0042ABFE" modified="2011-05-03" version="1" precursor="true">MEKRPIGISFVSVLVFVVALISLVVGISLFVVGTPLDLLWTVKSSLSLSIRGTLVGNIFGIFLLLLGTVLMASGYGLLKGNKIAWWAVIVIFMVNAVGDLASVIMGNIDSISGVIIVGILVLYLTRPHVRSYFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T855</accession>
    <name>F0T855_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645032.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1443"/>
    <dbReference type="EnsemblBacteria" id="ADZ09681">
        <property type="protein sequence ID" value="ADZ09681"/>
        <property type="gene ID" value="Metbo_1443"/>
    </dbReference>
    <dbReference type="GeneID" id="10277894"/>
    <dbReference type="KEGG" id="mel:Metbo_1443"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="RHCEMIQ"/>
    <dbReference type="OrthoDB" id="60124at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="24826" checksum="7DB4D97AEFC429BE" modified="2011-05-03" version="1">MEYAEKVKEFKELLQTKGSPVAVKLVKSPDEIPEGYSKISANKRHCEYVQDARLEGLEGYAGAEEHLCKGGAGVMGITAIPEPVATGKMYHKLGNYQTLEASKETVNSIPKSDENYYASLYSPLETAKFEPDVIILVVNPKQALKISQALLNKKGGRLSSDYSGIQSVCADAVVAVKQRGVSNMTLGCNGSRKYAEIGEDEVIIGIPANQFNEMVDAVSTFKEKWGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E1</accession>
    <name>F0T8E1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645043.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1455"/>
    <dbReference type="EnsemblBacteria" id="ADZ09692">
        <property type="protein sequence ID" value="ADZ09692"/>
        <property type="gene ID" value="Metbo_1455"/>
    </dbReference>
    <dbReference type="GeneID" id="10277906"/>
    <dbReference type="KEGG" id="mel:Metbo_1455"/>
    <dbReference type="eggNOG" id="arCOG02572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_6_0_2"/>
    <dbReference type="OMA" id="YWGEMSE"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="21224" checksum="AD7DC488C43D5ABE" modified="2011-05-03" version="1">MKVMVLTSSPNEDGLTESCARAAKKGIENGNSKAVMVRLNDLNILKCKACDQGWGICLKGTCILEDDFEKVHEEMGEVDGYVVVTPVYFGDMSESAKTFFDRLRRCEANRLMREKGNKIHNKPFICVAAAGGSGNGTLSCLTNMERVFLHMNNMDYTNMPKFDYIGVTQRNKEYMLKAIESSALNLVKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H2</accession>
    <name>F0T8H2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645074.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09723">
        <property type="protein sequence ID" value="ADZ09723"/>
        <property type="gene ID" value="Metbo_1490"/>
    </dbReference>
    <dbReference type="GeneID" id="10277941"/>
    <dbReference type="KEGG" id="mel:Metbo_1490"/>
    <dbReference type="HOGENOM" id="CLU_2893264_0_0_2"/>
    <dbReference type="OrthoDB" id="141907at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="62" mass="7293" checksum="DF856A09DA87B3CE" modified="2011-05-03" version="1">MKHDNVLSDVFYNVEPEKIIGKKLIEYENWQTERLADTGNLSGPQPNLTLIFEDGYYMEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J6</accession>
    <name>F0T8J6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1516</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645098.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1516"/>
    <dbReference type="EnsemblBacteria" id="ADZ09747">
        <property type="protein sequence ID" value="ADZ09747"/>
        <property type="gene ID" value="Metbo_1516"/>
    </dbReference>
    <dbReference type="GeneID" id="10277967"/>
    <dbReference type="KEGG" id="mel:Metbo_1516"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="ADRANDW"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="25100" checksum="1EC2DE6168A2A906" modified="2011-05-03" version="1">MNNENIIEIKDLKKGYDNGKIKALNGMNLNVKKGEFISIMGPSGSGKSSLLNMIGGLDVADEGTINVAGIDMMKTKNLNKFRSKEIGFVFQMHNLIPNLTVVENVEIPMYETNTSSKDMRKKALALLKSVGLEDKVDQKPTKLSGGQRQRVAIARALVNNPSIILADEPTGSLDSKTGEVILNLLKDLHAKENVTLVMVTHEPYVGNMAERIVTVLDGKCLSDKKTSEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J8</accession>
    <name>F0T8J8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645100.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09749">
        <property type="protein sequence ID" value="ADZ09749"/>
        <property type="gene ID" value="Metbo_1519"/>
    </dbReference>
    <dbReference type="GeneID" id="10277970"/>
    <dbReference type="KEGG" id="mel:Metbo_1519"/>
    <dbReference type="HOGENOM" id="CLU_1954683_0_0_2"/>
    <dbReference type="OrthoDB" id="143069at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14351" checksum="8FAE9E488DC75BB5" modified="2011-05-03" version="1">MVGIGLYPIFGRPFVIYLGVLTLASFFLTAVFGFSYYRGWLKFKWHPTMVVISFILAMLMTFIGLSLHKPLVGTLGILALFSFIIASLIGFGIHQRKFSLQFKWHPGLVIFAFIFAVLHGVVGVLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N7</accession>
    <name>F0T8N7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="3 9">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cofG</name>
        <name evidence="11" type="ordered locus">Metbo_0327</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="3 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643930.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0327"/>
    <dbReference type="EnsemblBacteria" id="ADZ08579">
        <property type="protein sequence ID" value="ADZ08579"/>
        <property type="gene ID" value="Metbo_0327"/>
    </dbReference>
    <dbReference type="GeneID" id="10276764"/>
    <dbReference type="KEGG" id="mel:Metbo_0327"/>
    <dbReference type="eggNOG" id="arCOG00657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054174_0_0_2"/>
    <dbReference type="OMA" id="TTACRYT"/>
    <dbReference type="OrthoDB" id="52096at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016841">
        <property type="term" value="F:ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="36"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="362" mass="40609" checksum="02BE789696EDD471" modified="2011-05-03" version="1">MKLLAKSEVESLLTGDYKSLIANLEAANSHRNSNEITFSKNVFLPITDICRNICGYCTFRRDPDSKDATILMKPDSIMKQLKTADSFNCREALFAFGEQADSEAVVMNELEALGFNNIVDYLHHMCDEALKKTGLLPHSNPGVLSKGELKQLREVNASMGLMLETTSQKLMNTVVHEQSPGKQPKLRIKSIENAGKLKIPFTTGLLIGIGETIEDRAESLLELRRLQNKYGHIQEIIIQNFKPKHGIPMENHGEPTLIEMIKMVTVTKLMFPDVSVQVPPNLNNQHSPIFLIAGADDWGGVSPVTPDFVNPEAPWPEVDELKKLTEELGFKLKERLPVYPDYINEEFLDSRILKKIEGMGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P0</accession>
    <name>F0T8P0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643933.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0330"/>
    <dbReference type="EnsemblBacteria" id="ADZ08582">
        <property type="protein sequence ID" value="ADZ08582"/>
        <property type="gene ID" value="Metbo_0330"/>
    </dbReference>
    <dbReference type="GeneID" id="10276767"/>
    <dbReference type="KEGG" id="mel:Metbo_0330"/>
    <dbReference type="eggNOG" id="arCOG04872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108341_0_0_2"/>
    <dbReference type="OMA" id="CTEGMLD"/>
    <dbReference type="OrthoDB" id="68097at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016736">
        <property type="entry name" value="MJ1481_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09873">
        <property type="entry name" value="DUF2100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="25491" checksum="F7B1F73473688437" modified="2011-05-03" version="1">MENFRIKQAENLLKSAAKNKTKNKELKNAKPGFINVDIYESVINNLLEAEEFIYSSRPIHKLDSEDATKFISKILASRKDIDVILSDFGVIDKTAPEEDVKENFKDYLILTTKNNFKKTISKFGIDTQKIVVAGVPLVPEDMQILNPKMPESALGPIAKKIQHVKNDIERKMEQFQLENMVVLVESDKSGELLSKRAKEMYNAKIVVVENLKDITVEEFISIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P3</accession>
    <name>F0T8P3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643936.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0333"/>
    <dbReference type="EnsemblBacteria" id="ADZ08585">
        <property type="protein sequence ID" value="ADZ08585"/>
        <property type="gene ID" value="Metbo_0333"/>
    </dbReference>
    <dbReference type="GeneID" id="10276770"/>
    <dbReference type="KEGG" id="mel:Metbo_0333"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079270_3_0_2"/>
    <dbReference type="OMA" id="MDFERAF"/>
    <dbReference type="OrthoDB" id="96137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="25070" checksum="C6D2A351E43765DA" modified="2011-05-03" version="1">MDVGDIISDSIKYPSSSWGKVLILGIITIASILIVPFFLVLGYMFRIIKATLAGIDELPEFDEIGDMFVDGLKVFVVGFVYAIPVWILSAIVGLVTGGNSAAAVTSLGTGYALAFILGNIVFFIIAVIIGLIELMAIANMAYNDGDLGTAFRFSEILDIIATIGWGKYIITYIVIVILAAIGVMIGVFTMFIIIGIILLPLIILPYLIMFEARGIGLLFSEAMETSAEEPLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P5</accession>
    <name>F0T8P5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643938.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0335"/>
    <dbReference type="EnsemblBacteria" id="ADZ08587">
        <property type="protein sequence ID" value="ADZ08587"/>
        <property type="gene ID" value="Metbo_0335"/>
    </dbReference>
    <dbReference type="GeneID" id="10276772"/>
    <dbReference type="KEGG" id="mel:Metbo_0335"/>
    <dbReference type="eggNOG" id="arCOG09164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052492_2_0_2"/>
    <dbReference type="OMA" id="GFHAANN"/>
    <dbReference type="OrthoDB" id="80877at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="33423" checksum="0AE57EFED5675CD8" modified="2011-05-03" version="1">MSNIEFLNNAHLGKNKPMNYFLTIVITIFGGSIAAIIFIILLMFIYLFIPGINAQTQFDNLGANPLLLLMLVGLVYGLYAMLFYLCIRFIHKKNFHILINTGKKIKWKRILIGAALWTGIMGAFTLLSLIFQGNSLSINFDYPSLFYLIILSLLVFPLQASFEEIFFRGYLMQGISLIFKKPVVSLVLTTVIFASIHYFNGSSISNSISIVISAMILGLMLGVIVLGENGLETAIGVHIANNFFVAVILNSSDSGMGNLPSLITAQSTDPYSGIPLLLLMALITLTILFWNKKSNISRIFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S3</accession>
    <name>F0T8S3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643966.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0363"/>
    <dbReference type="EnsemblBacteria" id="ADZ08615">
        <property type="protein sequence ID" value="ADZ08615"/>
        <property type="gene ID" value="Metbo_0363"/>
    </dbReference>
    <dbReference type="GeneID" id="10276800"/>
    <dbReference type="KEGG" id="mel:Metbo_0363"/>
    <dbReference type="eggNOG" id="arCOG04866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541447_0_0_2"/>
    <dbReference type="OMA" id="VMARCMN"/>
    <dbReference type="OrthoDB" id="49937at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008303">
        <property type="entry name" value="Methan_mark_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09887">
        <property type="entry name" value="DUF2114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016937">
        <property type="entry name" value="UCP016937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03285">
        <property type="entry name" value="methan_mark_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="488" mass="52931" checksum="B927FBBAA7257D94" modified="2011-05-03" version="1">MSLLGNLFGRGSKPIFAKSHYIYTETIKSTPFMKKTAGSGLNMKRDIYYVVASVELGNTTTKCILTATNLTTSKTYLLDKTVKMTRDIRPPKEGEKVFGETVWEVELTKESVSEMVRDTILESLKKAHVDVEEDLDFVVRSTGVTAGFATPDEVGELIIALAKGCLEAGIPPRKMAPAMSKDNLRKDLQEESYLDQVMFDGAVVGVLPPTGRVVVANEMEGELVTAGIKVASNWTNVDFRNPCVSMDFGTTLAGRITNNDKPYARTIGNFCGLAGAISDSIIRGTEKVDKRGGAALDLYEKGIIKGADWKAAAKYATEIHEHVDIRKVPENRERFGTVPVDPKAAYEAGTTLIGCDVGKDGDEIPTLVKLGHEIFETSGIHTLFATLDHVSTNVVKRLIEEAFEEGVIEEGSMLGVTGRAGITGKKPELILDFSKDYFKETIFVSDALAMGSAMMARCMNSLGTPTKPVGGSQGGPCILGPRRKLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T0</accession>
    <name>F0T8T0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogen dehydrogenase</fullName>
            <ecNumber evidence="3">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643973.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0370"/>
    <dbReference type="EnsemblBacteria" id="ADZ08622">
        <property type="protein sequence ID" value="ADZ08622"/>
        <property type="gene ID" value="Metbo_0370"/>
    </dbReference>
    <dbReference type="GeneID" id="10276807"/>
    <dbReference type="KEGG" id="mel:Metbo_0370"/>
    <dbReference type="eggNOG" id="arCOG01549">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="VQNNPAM"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="470" mass="52462" checksum="C0C57030A0646982" modified="2011-05-03" version="1">MVKLTMEPVTRIEGHAKITVHLDDAGNVEETRLHVMEFRGFEKFLQGRPVEEVPRIVPRICGICDVQHHLAAAKASDQCYGFKDDEILPAAYKMRELMNWGSFMHSHALHFYFLAAPDFIAGKDRKTRNVFQIIKDAPDVALQAIELRKNALDIVKATGGRSIHPTSSTPGGISTELDAETQADLLKKAQRNVELAQATFDLAKPIFEENLELVETLGAVETYHTGLVNKGVWDVYNGDVRMKDKEGKIYAEFGSENYLDYMAEKVKPYSWLKFPYIKDLGYPEGIYRVAPLSRLNVADKMPSPIAQDAFKEFKETFGYAQQPLLFHWARLIELIAAAEMAADALEGDLSGEKFPGPIERQAGEGVGIVEAARGTLTHHYQTDEDGLVTKANIVVATIQNNPAMEMGIQKVAKDYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTIDSNMKLATVEVYDGQGHLINKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W6</accession>
    <name>F0T8W6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645145.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09794">
        <property type="protein sequence ID" value="ADZ09794"/>
        <property type="gene ID" value="Metbo_1567"/>
    </dbReference>
    <dbReference type="GeneID" id="10278023"/>
    <dbReference type="KEGG" id="mel:Metbo_1567"/>
    <dbReference type="eggNOG" id="arCOG10898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147142_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="12725" checksum="7A2A22F9CEFBB630" modified="2011-05-03" version="1">MVEIKWTSVRRGLIISLILWAILRDIAGDAGGIIGFFIATAYVGYNVGEGYKNGAIHGALVGVVGGVVGTLIILILYLLGFGALAKELWPVTSLLQALIAIIIYALIGAVGGTIGSIFNRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X0</accession>
    <name>F0T8X0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cytoplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645149.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1571"/>
    <dbReference type="EnsemblBacteria" id="ADZ09798">
        <property type="protein sequence ID" value="ADZ09798"/>
        <property type="gene ID" value="Metbo_1571"/>
    </dbReference>
    <dbReference type="GeneID" id="10278027"/>
    <dbReference type="KEGG" id="mel:Metbo_1571"/>
    <dbReference type="eggNOG" id="arCOG09742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072734_0_0_2"/>
    <dbReference type="OMA" id="IPVSCTE"/>
    <dbReference type="OrthoDB" id="101533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="330" mass="37205" checksum="531FFD2DB12E82E0" modified="2011-05-03" version="1">MNADLIEYFEELEFGEIQHQGSMAVLPVFTSHESSLHYMTLTNALKADLVEISEMNEMGTVSELKVHNSADVPVLILDGEELVGAKQNRIVNTTILLKERSLTVIPVSCVEQGRWSYKSRKFQDSSRLAHSKLRNLKSVSVKKSVEEYGQHFSDQGELWNAIHEIERKTDLHSPTSAMTDIYDEFKDDLTDYLESFKVVEGQTGLLVFINGEIMGLDIISNKSAYKVLHKKLVESYALDSMFQDNKKTSMNINLEAAHKFIEDIKDCDESKNESVGYGFDHRFASDLYIGSALVFNNELIHASFFKSLEVEDGEIGGMARSTVRANLKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T951</accession>
    <name>F0T951_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mov34/MPN/PAD-1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0421</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644024.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0421"/>
    <dbReference type="EnsemblBacteria" id="ADZ08673">
        <property type="protein sequence ID" value="ADZ08673"/>
        <property type="gene ID" value="Metbo_0421"/>
    </dbReference>
    <dbReference type="GeneID" id="10276858"/>
    <dbReference type="KEGG" id="mel:Metbo_0421"/>
    <dbReference type="eggNOG" id="arCOG01139">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116578_1_0_2"/>
    <dbReference type="OMA" id="HPNEYMG"/>
    <dbReference type="OrthoDB" id="118690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="12"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="135" mass="15055" checksum="3B933439AAD5D3D4" modified="2011-05-03" version="1">MFGLGKKKYKEVHVDHELVEEIIDIAKESYPNEFSAMLHGLVEDDILKIDELIFLPGAASESGAVMEIFMLPMLSDDVGSVHSHPGYSAEPSGADLQFFSKRGTFHMIIALPYTEDSIIAYNSYGEQIPFSIVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T954</accession>
    <name>F0T954_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644027.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0424"/>
    <dbReference type="EnsemblBacteria" id="ADZ08676">
        <property type="protein sequence ID" value="ADZ08676"/>
        <property type="gene ID" value="Metbo_0424"/>
    </dbReference>
    <dbReference type="GeneID" id="10276861"/>
    <dbReference type="KEGG" id="mel:Metbo_0424"/>
    <dbReference type="eggNOG" id="arCOG04851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1113868_0_0_2"/>
    <dbReference type="OMA" id="LDEHYTD"/>
    <dbReference type="OrthoDB" id="93960at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="27434" checksum="F704A99C53234F28" modified="2011-05-03" version="1">MFDGSIKISNKDIPRTLLGSSPFVAAPQFGHRARLYQLDLYNNPENIAKIIQKSHDMGVKGIQVMPEPPVIEAIGLARDAGIKMDIVGTLMPETEAEDIELFSDLGAVSVMIHGTLTDTENWDAIEEKLLKIKEIEAVPGLVTHSPFKTTSSLLKSDVLDLFDIYSVPVNKLGYLMDCDVYDKEVRANLDNMMEELNKTIMVRNVLAAGVLMPEDAFNYLKTVSFADVVALGIASVDEAQETFSLLKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T979</accession>
    <name>F0T979_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644052.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0449"/>
    <dbReference type="EnsemblBacteria" id="ADZ08701">
        <property type="protein sequence ID" value="ADZ08701"/>
        <property type="gene ID" value="Metbo_0449"/>
    </dbReference>
    <dbReference type="GeneID" id="10276887"/>
    <dbReference type="KEGG" id="mel:Metbo_0449"/>
    <dbReference type="eggNOG" id="arCOG02579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159067_0_0_2"/>
    <dbReference type="OrthoDB" id="80510at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="28352" checksum="979C57A957BFA136" modified="2011-05-03" version="1">MLLEELHDRDYIDSLFKQYQELADEQPNLNSELFIFEKMVSKRVKKFLKKSNRLKDVEKKDLKEIVHFSSIVFTEGSLVAALRLDLCNRCGWCCENCSPIYITAEEYESYQKSENIMAGEMVPHEDGYRFEQDRPCEHFIRSTKKCDIYNERPAVCKAYPIMIRDEKYVFTPNHFCKYAVEFVVQKAITEVTTCLKIREDPEFLEKLKKQMETQIPDDADNLDERVKKWNKIAEELDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T981</accession>
    <name>F0T981_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645183.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1605"/>
    <dbReference type="EnsemblBacteria" id="ADZ09832">
        <property type="protein sequence ID" value="ADZ09832"/>
        <property type="gene ID" value="Metbo_1605"/>
    </dbReference>
    <dbReference type="GeneID" id="10278062"/>
    <dbReference type="KEGG" id="mel:Metbo_1605"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381413_0_0_2"/>
    <dbReference type="OrthoDB" id="9990at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="2">
        <location>
            <begin position="107"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="21830" checksum="DF53A46BDEFBDB2B" modified="2011-05-03" version="1" precursor="true">MEGYRGNCLKRIVSLVFICGMFIAMTGVSNLAYAADNSVNNGVKVDGYYECEHPYITELAANITQNVTKNSTDSRYNIVKAIFKWMHSNVKYEKPMYYNSKHYAVQTAVLGEGNCCDQARLFIALCRAAGIPHNATKFDYSGAVQFMGGSVYGHVWPVVLLENGTQLICDTSSKTSKLGAPTWKNRGSVSETYDLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B9</accession>
    <name>F0T9B9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645221.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09870">
        <property type="protein sequence ID" value="ADZ09870"/>
        <property type="gene ID" value="Metbo_1644"/>
    </dbReference>
    <dbReference type="GeneID" id="10278101"/>
    <dbReference type="KEGG" id="mel:Metbo_1644"/>
    <dbReference type="HOGENOM" id="CLU_147142_0_0_2"/>
    <dbReference type="OMA" id="IVEYGTF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="12615" checksum="76C4B38193F8E730" modified="2011-05-03" version="1">MLNWKYIFLGATLVVVLGLFLRIVEYGTFIGMFVPGIIVGYLVNNDYKNGAKNGIITGIIGGFILGILLVIPLLNLPSANHIIFYLFVGGIINAIFTMILDAIGGIIGVIIRNKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I5</accession>
    <name>F0T9I5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0481</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644084.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0481"/>
    <dbReference type="EnsemblBacteria" id="ADZ08733">
        <property type="protein sequence ID" value="ADZ08733"/>
        <property type="gene ID" value="Metbo_0481"/>
    </dbReference>
    <dbReference type="GeneID" id="10276921"/>
    <dbReference type="KEGG" id="mel:Metbo_0481"/>
    <dbReference type="eggNOG" id="arCOG00232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_2"/>
    <dbReference type="OMA" id="RIGNHAS"/>
    <dbReference type="OrthoDB" id="76473at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="25620" checksum="97E02C78940EAC7C" modified="2011-05-03" version="1">MYVTILENQLEKIKDTLLDYSNETSERITKSVEGFINEDVYRSKEIIEETNKINKKSEEIEYGCLKILGLQQPVAKDLRLGAAILRTSTELERINLLAAYVARYSIDSLKKNSNFFKPIHINLMSHTVQNMLKDGVGSLISEDIQLLKRSTKNFVSLQEFYNQMFMEYGEDKTSNSSMHSILVARGLLSMGHHIMGMDDRIAYSIIGKRIMHHKVFYNVLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K8</accession>
    <name>F0T9K8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elp3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0504</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644107.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0504"/>
    <dbReference type="EnsemblBacteria" id="ADZ08756">
        <property type="protein sequence ID" value="ADZ08756"/>
        <property type="gene ID" value="Metbo_0504"/>
    </dbReference>
    <dbReference type="GeneID" id="10276944"/>
    <dbReference type="KEGG" id="mel:Metbo_0504"/>
    <dbReference type="eggNOG" id="arCOG01360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="OMA" id="KRGPHNC"/>
    <dbReference type="OrthoDB" id="29724at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01210">
        <property type="entry name" value="TIGR01210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3" evidence="7">
        <location>
            <begin position="43"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="39849" checksum="8BEEC51D15640272" modified="2011-05-03" version="1">MEIENLTKDIRENAIAKMEKRSAKELAASWSGEDILYSGKGNAIYMVLPTSGCAWAVSESGGCSMCSYVADSPLEDVPSEELIDIFKDRFSRFDITEKTAVKIFVSGSFLNPVEIPPSVRNEILGILKNEELVEEVVVESRPEFITETVLKECCEALGDKIFEIGIGLETSNDYTREKKINKGFTREDFEKAVEAIKNSELKCDVRAKAYLFVKPILTSEKDAIEEAISSAFYAESAGASRISFCPATIHKDTLMELLWKKGSYQPPWIWSVMEIIQRVRSEVKIPIIMDTAGFGSSRGPYNCKKCNSKLKAMIIESNLNQSLPETIECECKSKWQAEVDYSNITRSTTRISRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M8</accession>
    <name>F0T9M8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645258.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09907">
        <property type="protein sequence ID" value="ADZ09907"/>
        <property type="gene ID" value="Metbo_1681"/>
    </dbReference>
    <dbReference type="GeneID" id="10278138"/>
    <dbReference type="KEGG" id="mel:Metbo_1681"/>
    <dbReference type="eggNOG" id="arCOG14008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784079_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="80" mass="9645" checksum="14763EB503ECE0C6" modified="2011-05-03" version="1">MDLEYLKSDTKRIEKKFEELNKKYDVIQREKELELLEKRSNAINEGSDDNLKTQIWGDIYKDVLEIEKKTDVKPDKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N5</accession>
    <name>F0T9N5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein UCP037409, membrane transporter, MTH672</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645265.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1689"/>
    <dbReference type="EnsemblBacteria" id="ADZ09914">
        <property type="protein sequence ID" value="ADZ09914"/>
        <property type="gene ID" value="Metbo_1689"/>
    </dbReference>
    <dbReference type="GeneID" id="10278146"/>
    <dbReference type="KEGG" id="mel:Metbo_1689"/>
    <dbReference type="eggNOG" id="arCOG04841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096712_0_0_2"/>
    <dbReference type="OMA" id="CPCCFGA"/>
    <dbReference type="OrthoDB" id="93238at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017199">
        <property type="entry name" value="UCP037409_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09930">
        <property type="entry name" value="DUF2162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037409">
        <property type="entry name" value="UCP037409_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="25366" checksum="1B30C435AEB1DEE8" modified="2011-05-03" version="1">MIELLWQLGILSAILLFGVKIGLAMGFSGLSKKVAVLIALGYGVGIFLLSTVITDSSSTVYKTIIDYNFVMFSVMSIIIIYAGVHTIREWKVNQKNHAKATCAAMIAPCPCCFTAVIAAIVIASPIIGASSAMIGRYAAVLMTIIILVVYFSSDIIVRVVNKPYPILLGNLMLFAGIYFLSSVILLPNINQVLMSPMKPYDLNSVGPIVYVMVFMIILAVIGFVKTKKESIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q3</accession>
    <name>F0T9Q3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645283.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1707"/>
    <dbReference type="EnsemblBacteria" id="ADZ09932">
        <property type="protein sequence ID" value="ADZ09932"/>
        <property type="gene ID" value="Metbo_1707"/>
    </dbReference>
    <dbReference type="GeneID" id="10278164"/>
    <dbReference type="KEGG" id="mel:Metbo_1707"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_0_2"/>
    <dbReference type="OMA" id="DAYHEFE"/>
    <dbReference type="OrthoDB" id="90050at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="32"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="21190" checksum="2B303C55E856DF89" modified="2011-05-03" version="1">MFDEFRLKMLNKKASTKDSNPDEIVENLGINKGDIIGDIGAGGGYFTLEFSKKVGGNGKVYAIDTNQKSLDYISKNNTLNNIETKLVEDDEFVLPEKVDIFFLRNVFHHLQDPVEYFKNLSVYLKNEGLIVVIDYKKKGFSFVGMFGHYTSEDDIIKQLEAAGFGVLKQYDFLQDQSFIKFKRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA19</accession>
    <name>F0TA19_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644193.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0591"/>
    <dbReference type="EnsemblBacteria" id="ADZ08842">
        <property type="protein sequence ID" value="ADZ08842"/>
        <property type="gene ID" value="Metbo_0591"/>
    </dbReference>
    <dbReference type="GeneID" id="10277036"/>
    <dbReference type="KEGG" id="mel:Metbo_0591"/>
    <dbReference type="eggNOG" id="arCOG01189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_2_2"/>
    <dbReference type="OMA" id="RRDYKRV"/>
    <dbReference type="OrthoDB" id="15948at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="129"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="550" mass="62596" checksum="656EB73C76422305" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA24</accession>
    <name>F0TA24_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08847">
        <property type="protein sequence ID" value="ADZ08847"/>
        <property type="gene ID" value="Metbo_0596"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0596"/>
    <dbReference type="eggNOG" id="arCOG15224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_215165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="50" mass="5564" checksum="16927511593719D2" modified="2011-05-03" version="1">MNSNTKKEIMGIIAIIVGIIFIVEPVVLPWLLGILLIIYGILELLNRGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA75</accession>
    <name>F0TA75_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10025">
        <property type="protein sequence ID" value="ADZ10025"/>
        <property type="gene ID" value="Metbo_1803"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1803"/>
    <dbReference type="eggNOG" id="arCOG11274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860704_0_0_2"/>
    <dbReference type="OrthoDB" id="112398at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17085" checksum="FC918E9418BC0FB6" modified="2011-05-03" version="1">MGLCLGFYGDVQVTMLGMTKRQFLKRTKECLDAQGIHVLNISELIDLERSGKINEKESYERIMAIMKDIESIFAGYERLNPPSDCYDTKSKILTSIILLQEAAAAFYEYILTIIKYQSKDSSKLEKAYLLLNKFREAFKPLNNSINPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB8</accession>
    <name>F0TAB8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644221.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0619"/>
    <dbReference type="EnsemblBacteria" id="ADZ08870">
        <property type="protein sequence ID" value="ADZ08870"/>
        <property type="gene ID" value="Metbo_0619"/>
    </dbReference>
    <dbReference type="GeneID" id="10277064"/>
    <dbReference type="KEGG" id="mel:Metbo_0619"/>
    <dbReference type="eggNOG" id="arCOG01895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="MDEGLHY"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="48"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="251" mass="27994" checksum="31D84A10D34935ED" modified="2011-05-03" version="1">MKIGVSTLALSPTPLETVLTGLEDRKVEYCEIMNEYPYNDIDSDVVGSYNLKISIHSPLSDVNIASYNDRMRTSSVSAIKNSIDTAVSVGSDVVVVHPGQVPILGKKFIDRIKEHSLESLRECSNYADENGIMICIENMPDFEELLLRDLNEVHNVAEELDSHITLDVGHAYNMNFSVEEMLSSPRIKHIHLSDNDGSYDSHDAIGSTNIDFKSLFKELKSVNYDGILVVEVKDPAALTESLDYIKNELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC1</accession>
    <name>F0TAC1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="8">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisS</name>
        <name evidence="10" type="ordered locus">Metbo_0622</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644224.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0622"/>
    <dbReference type="EnsemblBacteria" id="ADZ08873">
        <property type="protein sequence ID" value="ADZ08873"/>
        <property type="gene ID" value="Metbo_0622"/>
    </dbReference>
    <dbReference type="GeneID" id="10277067"/>
    <dbReference type="KEGG" id="mel:Metbo_0622"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="47919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="429" mass="48219" checksum="2D3C0D58F5B059C8" modified="2011-05-03" version="1">MELQRPRGTRDFLFEEMKERKNVENTMKRIFESYGYGEIKTPIFEELSLFTTKSGEGIKDQIYHFQDKSGRDLALRPELTAPVARMYIKELQKAPKPVKMYYFGSCFRYERPQAGRFRQFWQLGCELIGGKSPGSEAEVISMAAHCLDELGLENYEIHIGNLGILRNILIDAQILGDTQDQVMAAIDKGDAEELKNLLKSINTDEDSEELLLKLIDMKGDASILKDAEALINCSENACNALFELEKLLEALEAFGFSNYTVNLGIARGLDYYTGTVFEIYVHGLGAQKQISGGGTYNLIELFGGEKVESTGFAFGFDRVMEALKKQQTTIDKEEAVKVFVAPLNSDLSLTGYEIVQKLRKANIGAEVDLVGKKLKKILSYADTKQISYVVLLGSREFEEGKVTVKDMNTGEQESVNISEVESYFKTMLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE9</accession>
    <name>F0TAE9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZPR1-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644252.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0650"/>
    <dbReference type="EnsemblBacteria" id="ADZ08901">
        <property type="protein sequence ID" value="ADZ08901"/>
        <property type="gene ID" value="Metbo_0650"/>
    </dbReference>
    <dbReference type="GeneID" id="10277095"/>
    <dbReference type="KEGG" id="mel:Metbo_0650"/>
    <dbReference type="eggNOG" id="arCOG04265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="OMA" id="HSAIIGD"/>
    <dbReference type="OrthoDB" id="109705at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="Znf_ZPR1-type_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="PTHR10876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="zf-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zpr1" evidence="5">
        <location>
            <begin position="6"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="187" mass="20744" checksum="3CDAED6578305899" modified="2011-05-03" version="1">MNNISTDCPVCKGRNTLEMTSKTEEIPYFGEILESTIICSECGYRHTDTICLEQKEPVYYSLLISKANINARVVKSQTATLSIPDLGLKVEPGPKSQGYVSNVEGVLNRFESAVETAIEWAEEPEVKENAIKIIEKIEDIKSGDGSATLIIEDPFGHSVILHDDAKHRKLSQEEVENLNTGFVTIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL8</accession>
    <name>F0TAL8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1869"/>
    <dbReference type="EnsemblBacteria" id="ADZ10090">
        <property type="protein sequence ID" value="ADZ10090"/>
        <property type="gene ID" value="Metbo_1869"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1869"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25810" checksum="F8D6FFA1CD0B1F97" modified="2011-05-03" version="1">MEKINILVVEDENILALGLKKKLENLGYAVTGMADSGEETFRQIEANVPDLILMDIVIKGEMDGIETAAKLNKSYSIPVIYLTAYADDEILKRAARTEPYGYILKPYKEKELKANIEMAIYRKKSENDETFDFEDIYKETTSFINKNEKPFKKILMGSYYDEVEVSVDVGTSKLYISAARNQQTSDGQDVSEVLSKIAFNYLERYAGEALIYPKGDEICLEVDKPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP8</accession>
    <name>F0TAP8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645471.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1900"/>
    <dbReference type="EnsemblBacteria" id="ADZ10120">
        <property type="protein sequence ID" value="ADZ10120"/>
        <property type="gene ID" value="Metbo_1900"/>
    </dbReference>
    <dbReference type="GeneID" id="40927934"/>
    <dbReference type="KEGG" id="mel:Metbo_1900"/>
    <dbReference type="HOGENOM" id="CLU_1782510_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="16552" checksum="4B2B46BDC865E759" modified="2011-05-03" version="1">MNYNDFSCPLCKQKLEFHGELFKCSCGNTFNLLDDIPYFWVDNGKTREMDAYFDNVPEQYESVNYLSRIYQFFGGLATQPEDLMVPIINQGIQDGLNSILKPQHQSILDLACRYRNVHPKSFKGSFSCMGSGFIHGNAKTSKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAW1</accession>
    <name>F0TAW1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase Metbo_0736</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644338.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0736"/>
    <dbReference type="EnsemblBacteria" id="ADZ08987">
        <property type="protein sequence ID" value="ADZ08987"/>
        <property type="gene ID" value="Metbo_0736"/>
    </dbReference>
    <dbReference type="GeneID" id="10277183"/>
    <dbReference type="KEGG" id="mel:Metbo_0736"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="OrthoDB" id="76909at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="7"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="232" mass="25757" checksum="417FC5021F432FC6" modified="2011-05-03" version="1">MNIQWLGHSAFLITTDENLRILIDPFISNNPTAPVTVEELYADVILVTHGHNDHFGDTMELINRTGALVVCNHELSIYLSRQGFETLGMNIGGTVQVQDINVTMVDAKHSSDFDFVDEVSAGGSAAGFIIELEDGKKLYHAGDTGLFSDMRNVISHIYSPQIAMLPIGDRYTMGPFEAAIAAEWIDPEIIIPMHYNTYPAIEQDPLNYSDLVRKSNRDVEVVILNPGDSYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX8</accession>
    <name>F0TAX8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalt transport protein CbiN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Energy-coupling factor transporter probable substrate-capture protein CbiN</fullName>
            <shortName evidence="10">ECF transporter S component CbiN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbiN</name>
        <name evidence="11" type="ordered locus">Metbo_0753</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CbiN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644355.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0753"/>
    <dbReference type="EnsemblBacteria" id="ADZ09004">
        <property type="protein sequence ID" value="ADZ09004"/>
        <property type="gene ID" value="Metbo_0753"/>
    </dbReference>
    <dbReference type="GeneID" id="10277201"/>
    <dbReference type="KEGG" id="mel:Metbo_0753"/>
    <dbReference type="eggNOG" id="arCOG04384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136197_2_0_2"/>
    <dbReference type="OMA" id="TEIEPDY"/>
    <dbReference type="OrthoDB" id="111394at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00330">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003705">
        <property type="entry name" value="CbiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662">
        <property type="entry name" value="PTHR38662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02553">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01165">
        <property type="entry name" value="cbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 10" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11852" checksum="2C3DCA36B9F1C102" modified="2011-05-03" version="1" precursor="true">MVNKRAILLLSLVVVIVVFPLAFYNGKGEAQGYFGGTDDQGPEYIESTGYTPWFHSIWEPPSGEIESLLFAVQAAIGAIIIGFVFGYYMGQDKERKRKLESKEKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY3</accession>
    <name>F0TAY3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chorismate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1909"/>
    <dbReference type="EnsemblBacteria" id="ADZ10129">
        <property type="protein sequence ID" value="ADZ10129"/>
        <property type="gene ID" value="Metbo_1909"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1909"/>
    <dbReference type="eggNOG" id="arCOG02098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131518_4_0_2"/>
    <dbReference type="OMA" id="MDYNKEV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="1.20.59.10">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
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        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11431" checksum="D6E00B2AF344B574" modified="2011-05-03" version="1">MDKEEASRLLQESRTKIDEMDELLIDLVEKRTLLARDIVNAKLVLGIEIEDKKREAYIHEKIKQIAREKNIDEVSLTSIIKILTDMSKKEQNKILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ6</accession>
    <name>F0TAZ6_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_1922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10142">
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        <property type="gene ID" value="Metbo_1922"/>
    </dbReference>
    <dbReference type="GeneID" id="10278380"/>
    <dbReference type="KEGG" id="mel:Metbo_1922"/>
    <dbReference type="eggNOG" id="arCOG14012">
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="16494" checksum="6F311CBA5C0FC262" modified="2011-05-03" version="1">MELKTLESERNKYLIMVSQEEKKIEEFESETSDEDVCKSINKCNQELEKIGVQVSDLNIKISEKTVTLEELQSERDELVKKSLMMLHSSLKKEHQRADKEHARYVELYTKERAKKHEIERKMMNLKMMVYHNYGLRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB23</accession>
    <name>F0TB23_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp fold domain protein, DUF201-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645520.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1949"/>
    <dbReference type="EnsemblBacteria" id="ADZ10169">
        <property type="protein sequence ID" value="ADZ10169"/>
        <property type="gene ID" value="Metbo_1949"/>
    </dbReference>
    <dbReference type="GeneID" id="10278409"/>
    <dbReference type="KEGG" id="mel:Metbo_1949"/>
    <dbReference type="eggNOG" id="arCOG01592">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059501_1_0_2"/>
    <dbReference type="OMA" id="DVNPRPT"/>
    <dbReference type="OrthoDB" id="58527at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
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        <property type="entry name" value="MfnD"/>
    </dbReference>
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        <property type="entry name" value="MfnD_preATP-grasp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="119"/>
            <end position="311"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="339" mass="37270" checksum="D9D9BA6619E83B0D" modified="2011-05-03" version="1">MKLLVFEYATASGVDDPFITVEGLAMLEGVLDDLKEFKPHYLVHSESSELISDSVPVVVKGDVRQWLTTHIKDYDACLPIAPEEDGILHDLTQIIERNGVKVFGSNSKAIQLTTDKFEMYKFLAGKVPVIKTEKIYFNQELEEMGRAAFQGSGLKVIKPADGVSSSGVMVVATLEEFLDGVNNIQKFTKLPYFVMQDYKPGVSVSVSLLSSGKTAVPISLNLQDMEIKSCKISYNGGTVPYDHDLSEQAKDIAQQAVELFEGSLGFVGVDLILGEDEVHLVEINSRLTTPYVALRRITNLNLARALMNSVDGELPVDIELDGQVKFYKEGKSLGVSVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB25</accession>
    <name>F0TB25_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645522.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1951"/>
    <dbReference type="EnsemblBacteria" id="ADZ10171">
        <property type="protein sequence ID" value="ADZ10171"/>
        <property type="gene ID" value="Metbo_1951"/>
    </dbReference>
    <dbReference type="GeneID" id="10278411"/>
    <dbReference type="KEGG" id="mel:Metbo_1951"/>
    <dbReference type="eggNOG" id="arCOG04219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027052_0_0_2"/>
    <dbReference type="OMA" id="MSHQALE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007408">
        <property type="entry name" value="DUF460"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707">
        <property type="entry name" value="PTHR40707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04312">
        <property type="entry name" value="DUF460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="239"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="475" mass="53815" checksum="B6C2C23119294B1F" modified="2011-05-03" version="1">MYKNTGSEGIKESNLIFKTQKSSRTTKGIIVGYDPGLTVGIAILNLNGELISLDSFKEIRRSEIISHIIGYGNTVLVATDVYPAPKTVRKIATILNSKIWSPYKNMSVESKIEIVDSFTAMGNSLNVPQNAHERDSLAAAVKTYKDHLNKFRQIEKRAEQLSMTQAMIDEVKIRVINGKSISNSLREVSQENIDNGYIGNLNHKTRGSSKSCHDKDVLKLSTKDPETPKVELSPEELTISRLKNKLSSQKRYINELREKNQELEAEVKFQKTEVSKFQSKMDKLRNEYSRKILEKREFASKISMIKRLQNKYSQERSMRLELEKQLDPNFGLETIGEDAVELKIITSFTREGIRESSLLMKISKGDVVLLENSEGGGSNTAAILCEIGVKAVITRDKISDPAENVFINCMIPVIPEDSIGIERVAQGSYVESEDLDGQIKKWMEKTRKQQTTEDKNKLISLVDEYRAQRRRELDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB43</accession>
    <name>F0TB43_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645540.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1969"/>
    <dbReference type="EnsemblBacteria" id="ADZ10189">
        <property type="protein sequence ID" value="ADZ10189"/>
        <property type="gene ID" value="Metbo_1969"/>
    </dbReference>
    <dbReference type="GeneID" id="10278429"/>
    <dbReference type="KEGG" id="mel:Metbo_1969"/>
    <dbReference type="HOGENOM" id="CLU_1763886_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16537" checksum="DF8169276A029475" modified="2011-05-03" version="1" precursor="true">MKIEKISIFLAALLLLATASGTVAAANSNNSTNTTYNITNNITTKEQALSRVNYLENQIGELQFQIGTINKNINQKNDQIKNLNSRLLLACYKNDLGQYKLIECKINNLKSDINALEVQKTTLLNTRNSFSWEMEDLKIKWGIIDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB44</accession>
    <name>F0TB44_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645541.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10190">
        <property type="protein sequence ID" value="ADZ10190"/>
        <property type="gene ID" value="Metbo_1970"/>
    </dbReference>
    <dbReference type="GeneID" id="10278430"/>
    <dbReference type="KEGG" id="mel:Metbo_1970"/>
    <dbReference type="HOGENOM" id="CLU_1763887_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16512" checksum="4FC516AC717B36FA" modified="2011-05-03" version="1" precursor="true">MKKIMGMSVFAVMILSIIAGCSAANPNDTNPLVNVEMKTSSGLIFTMNENQTFTFKANDPFWLTATVQSNGQVISSNQNILFYDHDLTHDVTYNHIVPSGQEFSINTNENNAHDHKYQLDQGTHELKFVYKNNNNELVSTTFYAVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA9</accession>
    <name>F0TBA9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L18e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl18e</name>
        <name evidence="6" type="ordered locus">Metbo_0809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644411.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0809"/>
    <dbReference type="EnsemblBacteria" id="ADZ09060">
        <property type="protein sequence ID" value="ADZ09060"/>
        <property type="gene ID" value="Metbo_0809"/>
    </dbReference>
    <dbReference type="GeneID" id="10277258"/>
    <dbReference type="KEGG" id="mel:Metbo_0809"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OMA" id="WRLERPR"/>
    <dbReference type="OrthoDB" id="104254at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_L18e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_L18e_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="PTHR10934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="5">
        <location>
            <begin position="22"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="120" mass="12976" checksum="D3A30E1D67B66B8E" modified="2011-05-03" version="1">MVKLTKTNPEILKIVASLKEKSKKEDAAIWRVIANKLVRPTRKTAEVNISDINRHTSPDEIVLVPGKVLGNGTLDHKVNVAAMSFSTGAEEKIATAGGECMAILEVAEKNPKGTGIRIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE0</accession>
    <name>F0TBE0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645560.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1989"/>
    <dbReference type="EnsemblBacteria" id="ADZ10209">
        <property type="protein sequence ID" value="ADZ10209"/>
        <property type="gene ID" value="Metbo_1989"/>
    </dbReference>
    <dbReference type="GeneID" id="10278449"/>
    <dbReference type="KEGG" id="mel:Metbo_1989"/>
    <dbReference type="eggNOG" id="arCOG06513">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107220_1_1_2"/>
    <dbReference type="OMA" id="NTYAWFL"/>
    <dbReference type="OrthoDB" id="108225at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15149" checksum="88871DC13BF7098A" modified="2011-05-03" version="1">MISEESVKELFNYLKTGENVKFFSKVSDDVNWTVMGTHPLAGNYKTKEEFVKSTFLRLNKVLNEGVVLKVNQVLVKDATAVVEMESLSTALNGKPFNNTYCWIVRFENEMVVEVRAYVDSALVQMVLDENETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE4</accession>
    <name>F0TBE4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1993"/>
    <dbReference type="EnsemblBacteria" id="ADZ10213">
        <property type="protein sequence ID" value="ADZ10213"/>
        <property type="gene ID" value="Metbo_1993"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1993"/>
    <dbReference type="eggNOG" id="arCOG10346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127844_0_0_2"/>
    <dbReference type="OMA" id="PWVGTIN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="11959" checksum="A79ACA7BB747FE38" modified="2011-05-03" version="1">MSGVNNQQNTQPNFKTQSVTVIASGPWVGTINDASGTKNIMGTGNKTFQLSSYPGKIVVEFQKDGTKDSRDSNDSIIPNTSPLTVQLLSGDTIIANKTTTEDGGIVNISNNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF5</accession>
    <name>F0TBF5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolysaccharide synthesis ExoD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645575.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2005"/>
    <dbReference type="EnsemblBacteria" id="ADZ10224">
        <property type="protein sequence ID" value="ADZ10224"/>
        <property type="gene ID" value="Metbo_2005"/>
    </dbReference>
    <dbReference type="GeneID" id="10278465"/>
    <dbReference type="KEGG" id="mel:Metbo_2005"/>
    <dbReference type="eggNOG" id="arCOG10854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093444_0_1_2"/>
    <dbReference type="OMA" id="REEPWLP"/>
    <dbReference type="OrthoDB" id="120507at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010331">
        <property type="entry name" value="ExoD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41795">
        <property type="entry name" value="PTHR41795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06055">
        <property type="entry name" value="ExoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033239">
        <property type="entry name" value="ExoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="210" mass="22980" checksum="A36EF7F8B7F965BE" modified="2011-05-03" version="1">MSKESTTESTSKIISGVSEQIPEDGVNFREFLDLIGEQGGLISCLILVAPFLLPVSIPGSSLPFGLAIILINIAILTKTHPLIPKMVMEYRISQSTMVSLLNGMNRILKGLEKFVKPRLNIVTRPYMDQINNVFMIFCAFLLMLPLPVPLTDFLPAYSILFLTLGSVENDGYMVIAGYLMALVTAIYFLLIALLGISGIKALLSILGINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF7</accession>
    <name>F0TBF7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645577.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2007"/>
    <dbReference type="EnsemblBacteria" id="ADZ10226">
        <property type="protein sequence ID" value="ADZ10226"/>
        <property type="gene ID" value="Metbo_2007"/>
    </dbReference>
    <dbReference type="GeneID" id="10278467"/>
    <dbReference type="KEGG" id="mel:Metbo_2007"/>
    <dbReference type="eggNOG" id="arCOG00301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248276_0_0_2"/>
    <dbReference type="OMA" id="NMWLKAT"/>
    <dbReference type="OrthoDB" id="82034at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="24549" checksum="FE102E4025B8ECA9" modified="2011-05-03" version="1">MADSVDNQCKTLDEILESRRTVRNFSKEIPTRETVEQILRSGLQAPYAAQAVGESTVFRRFFVFENGTESMKTAEKLMRNKAEEGLNHFKGILKEQPMLKQRVGNFMERLQLFVDNGVIGVGTAPYFIVVAELRGIPPVEQESIAHCMENMWLKATELGLGFQIVSLTSQMSENQEFLDLLGIPLGPYEINGCAIGYPATEMSKVVRPDIKEVSQWID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG9</accession>
    <name>F0TBG9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645589.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2019"/>
    <dbReference type="EnsemblBacteria" id="ADZ10238">
        <property type="protein sequence ID" value="ADZ10238"/>
        <property type="gene ID" value="Metbo_2019"/>
    </dbReference>
    <dbReference type="GeneID" id="10278479"/>
    <dbReference type="KEGG" id="mel:Metbo_2019"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="OMA" id="SIWHETE"/>
    <dbReference type="OrthoDB" id="106121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="11215" checksum="BE1BD80F4E7A63D4" modified="2011-05-03" version="1">MPMKKILWWLIAGTKGGINRARILSLLNERPYNAHQLAEKLDLDYKTVRHHIKVLEDNNVITTSGEKYGTMYFLSSSMEENYELFQSIWHETEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI0</accession>
    <name>F0TBI0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645600.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2030"/>
    <dbReference type="EnsemblBacteria" id="ADZ10249">
        <property type="protein sequence ID" value="ADZ10249"/>
        <property type="gene ID" value="Metbo_2030"/>
    </dbReference>
    <dbReference type="GeneID" id="10278491"/>
    <dbReference type="KEGG" id="mel:Metbo_2030"/>
    <dbReference type="eggNOG" id="arCOG08826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152017_0_0_2"/>
    <dbReference type="OMA" id="VGHYDCA"/>
    <dbReference type="OrthoDB" id="111652at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="14300" checksum="C89D2370E398DC5E" modified="2011-05-03" version="1">MKTEFVTCLNCIDGRVQLPVINWILENYDVKYVDMITAPGINGLLSNRNNDVSDILEKVTFSNEGHSTELIFVVGHHDCLANPMDDETHNKQIIESVERIKELYSACDVVGLWVDDDFNVQIVCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ9</accession>
    <name>F0TBJ9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645619.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2049"/>
    <dbReference type="EnsemblBacteria" id="ADZ10268">
        <property type="protein sequence ID" value="ADZ10268"/>
        <property type="gene ID" value="Metbo_2049"/>
    </dbReference>
    <dbReference type="GeneID" id="10278511"/>
    <dbReference type="KEGG" id="mel:Metbo_2049"/>
    <dbReference type="eggNOG" id="arCOG02316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100860_0_0_2"/>
    <dbReference type="OMA" id="EPTVHMK"/>
    <dbReference type="OrthoDB" id="71317at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014424">
        <property type="entry name" value="UCP004897_ACT"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004897">
        <property type="entry name" value="UCP004897_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="18484" checksum="0A3109BADC4AAECF" modified="2011-05-03" version="1">MWDRIKHKFEKFPARMAVARKIVELGLRVGENGKIYLDDVEINDVALARSVNVDRRTIKSTVDVILADPQLSEIFQNIHPAGTLLKNIAKNLGFGVVEIEADAGNAGIIARSTELISIEGLSIRQAHAGDPELEEHPKLTIITEKPVKGNLINEFLKIPGVKRVSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK3</accession>
    <name>F0TBK3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2053"/>
    <dbReference type="EnsemblBacteria" id="ADZ10272">
        <property type="protein sequence ID" value="ADZ10272"/>
        <property type="gene ID" value="Metbo_2053"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2053"/>
    <dbReference type="HOGENOM" id="CLU_2839386_0_0_2"/>
    <dbReference type="OMA" id="MMAFVVI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="65" mass="6919" checksum="E3584FEC225AE1A8" modified="2011-05-03" version="1">MMIPMLKGMGVAKVGSVGKAASVGKIGFLGKLGLIGFFGLFGLGIIVLLIIAAVVYFLFIKRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK6</accession>
    <name>F0TBK6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2056</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645626.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2056"/>
    <dbReference type="EnsemblBacteria" id="ADZ10275">
        <property type="protein sequence ID" value="ADZ10275"/>
        <property type="gene ID" value="Metbo_2056"/>
    </dbReference>
    <dbReference type="GeneID" id="10278518"/>
    <dbReference type="KEGG" id="mel:Metbo_2056"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="GETVFFY"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="244" mass="27470" checksum="3F8A8624DF676069" modified="2011-05-03" version="1" precursor="true">MESDEYVIQTSSLTKKFGDFTAINDLNLKIKKGEIYGLLGPNGAGKTTAIRLLCGLINYGSGEAYVLGTKVPNHSIAHQIGYMPQETALYKGLSVQQNMEFFGEIYGLDKQRISERIDSLLEFIDLQNWRNELVENLSGGMKHRVSLVCALIHEPKLLFLDEPTVGVDPELRVSFWKYFNDLKNNGVSILITTHYMDEARHCDRIGFMKSGTLIAEDEPSQLLKITGKTSLEDAFLVFSREVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL9</accession>
    <name>F0TBL9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="4">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0846</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644447.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0846"/>
    <dbReference type="EnsemblBacteria" id="ADZ09096">
        <property type="protein sequence ID" value="ADZ09096"/>
        <property type="gene ID" value="Metbo_0846"/>
    </dbReference>
    <dbReference type="GeneID" id="10277295"/>
    <dbReference type="KEGG" id="mel:Metbo_0846"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_3_3_2"/>
    <dbReference type="OMA" id="LYRPKMG"/>
    <dbReference type="OrthoDB" id="12911at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="6">
        <location>
            <begin position="2"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="621" mass="73289" checksum="24F0E8C5D10B494E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM7</accession>
    <name>F0TBM7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0854</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644455.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0854"/>
    <dbReference type="EnsemblBacteria" id="ADZ09104">
        <property type="protein sequence ID" value="ADZ09104"/>
        <property type="gene ID" value="Metbo_0854"/>
    </dbReference>
    <dbReference type="GeneID" id="10277303"/>
    <dbReference type="KEGG" id="mel:Metbo_0854"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046109_0_0_2"/>
    <dbReference type="OMA" id="RNRWAEG"/>
    <dbReference type="OrthoDB" id="83144at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="49"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="395" mass="45735" checksum="AADE7A1108C4ED62" modified="2011-05-03" version="1">MDILIVILYIVMFLLVWQFVGYPALMGSIAFISRKYTKNNDNTFKTSVSILVPTYNEEKVIRLRLTNLMKLNYPNELYEIIVVDSGSNDKTKEIVEEIMDKQINLKPELKLAIQSERKGKASAINFGKQHCNGEIVLVTDANAIFEKNVLNEIIPCFKDSNVGGVGGRFVVSNPNANLTSSTQFYWTIEYIMRMGESILDSVCLFHGEMHAWRKGLIHCDEKIISEDLNIAIQLRKAGYKLKYAPDALVFEQTPNNIDEQIIQRTKTTVGTLQNIFKYWKFFIIPRNLYVAFIFPSHKILPMLSPFLLFSVLVLYILIWNINVILINIFICIMIFGCLLIYLLIIKSYFERNKVSGSFSLFKIVKYVLINEFIVLKGWKDYIFNNYSVLWEKVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU8</accession>
    <name>F0TBU8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Isopropylmalate/citramalate/homocitrate synthase</fullName>
            <ecNumber evidence="4">2.3.1.182</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645641.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2071"/>
    <dbReference type="EnsemblBacteria" id="ADZ10290">
        <property type="protein sequence ID" value="ADZ10290"/>
        <property type="gene ID" value="Metbo_2071"/>
    </dbReference>
    <dbReference type="GeneID" id="10278533"/>
    <dbReference type="KEGG" id="mel:Metbo_2071"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="OMA" id="QIEAGFP"/>
    <dbReference type="OrthoDB" id="26145at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="391" mass="43429" checksum="590BC106E7E94152" modified="2011-05-03" version="1">MQYLVSPFNKEANLKFPEKITVYDTTLRDGEQTPGVCLRTPEKLKIAKKLDEVGIHQIEAGFPVVSKEEKKSVTAIVNEDLNAQILCLSRTKKSDIDTAIDCDVDGIITFMSTSDLHLKHKIKMSREEILNVCMNSIEHAKDHGLFVAFSAEDATRTDLDFLKKMYKKADDYGVDRVHIADTVGAISPYGMDYLVKQLRSTINAEIALHCHNDFGMALANSIAGLLAGANAVSTTVNGIGERAGNAALEELVMTLKFIYGVDLGFDVSKFCELSKLVESLTHMDIPYNKPIVGKNIFRHESGIHVDAVIEEPLTYEPFLPELIGHRRQIVLGKHSGCRAVRAKLNECGIDVTKDELCKIVEQVKSKREEGNYINDELFNSIVASVRGPFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV5</accession>
    <name>F0TBV5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645648.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2078"/>
    <dbReference type="EnsemblBacteria" id="ADZ10297">
        <property type="protein sequence ID" value="ADZ10297"/>
        <property type="gene ID" value="Metbo_2078"/>
    </dbReference>
    <dbReference type="GeneID" id="10278540"/>
    <dbReference type="KEGG" id="mel:Metbo_2078"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="OMA" id="CLVSQRP"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAS-barrel" evidence="3">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="DUF87" evidence="2">
        <location>
            <begin position="125"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="511" mass="56736" checksum="254140777ABE3256" modified="2011-05-03" version="1">MTGEVIGRCVGETSLVDVSFISKEMPMMGEYVTMEYDGKKVLGMIEGLVRGSVSINNEIYDPKTIEKIRRMEGDDHYIRGNVRILGDVENNLRIPRTPAPPGTEIRSADSEILKKIFQVDVAGLKLGNLITQENVPVEVDINKMVSRHLAVLAMTGAGKSNTVSVIVNGLLNVNGSVLIFDMHSEYVNTDFGDDKVNILKPKINPIYMSFSEVKKLSNIPPNAYVQERYFRKAYNSAKNDLKKGGVGNDFIGLLLSKLESWLMEESDESDGKSANSADRKSITDVLNKVEDMKLKYGNILSLEAPNIIDSIKVAKANILDLGSVDEFASDVVVSHVLRTVLKSRKDYLRQGEGLKFPVFLILEEAHILAPQNRKTESKLWISRIAREGRKFSVGLCMVSQSPKSLDSDALSQANNMIILRLVEPTDQNHVQRASESLSDDLIAQLPSLNIGEAIILGLMTKIPTLVKIDEFKGKVSGGDLNVVEEWSKTAEKERSILEEQKREYEDLGGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV6</accession>
    <name>F0TBV6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NurA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645649.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2079"/>
    <dbReference type="EnsemblBacteria" id="ADZ10298">
        <property type="protein sequence ID" value="ADZ10298"/>
        <property type="gene ID" value="Metbo_2079"/>
    </dbReference>
    <dbReference type="GeneID" id="10278541"/>
    <dbReference type="KEGG" id="mel:Metbo_2079"/>
    <dbReference type="eggNOG" id="arCOG00367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056881_0_0_2"/>
    <dbReference type="OMA" id="YILKKSH"/>
    <dbReference type="OrthoDB" id="57919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="47"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="360" mass="42092" checksum="2EE7EF300FC01F39" modified="2011-05-03" version="1">MLDSLYQRALQKRDEINQKISDDFMDADVDPSQYWISHKIEEDDGNCSIAAGDGSINKKNFMGFIFYAIDAECLVFNDKLMKIGSSEIDIIPHHDFVEDRLRNYMGIFEVKNALRALNEHEIKYLLFDGSIMGNLIRPIPMEKRLKAEIKDKIKKKYVPRLVKSLDLDLKNSEVGITSSQFSEEMEEFEDPVNAMIYLESIENLLVIKKLLENKRSVMGISKTSTNREYFDYKIPDMAIFDRYSREEGFSKPRNLRIFDDDFKREEFPVANDFFKGLTFTIFYVRLENHKNILKFELPYKVDETELDQIKKLLGTIKNHSAEGYPLLLKKAHNDVVIKRKDLENLSKIIGFMEKSGREML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW9</accession>
    <name>F0TBW9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645662.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2092"/>
    <dbReference type="EnsemblBacteria" id="ADZ10311">
        <property type="protein sequence ID" value="ADZ10311"/>
        <property type="gene ID" value="Metbo_2092"/>
    </dbReference>
    <dbReference type="GeneID" id="10278555"/>
    <dbReference type="KEGG" id="mel:Metbo_2092"/>
    <dbReference type="eggNOG" id="arCOG00463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115699_0_0_2"/>
    <dbReference type="OMA" id="GCSDCRC"/>
    <dbReference type="OrthoDB" id="119956at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="PTHR37460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17420" checksum="4AAC4A056EAC91AE" modified="2011-05-03" version="1">MSKKRIYKGSYCLIISLSEDSVVKVGSLGNIDFKKGHYVYVGSALNSLESRLRRHLSHEKKLHWHVDYMLNHVNANLVDIVYVVDNGKWECSIAAQISEKGIETARFGCSDCKCGSHLFKFEDFETAVSCSSYSFETLSLHPILWSDGSNFESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX1</accession>
    <name>F0TBX1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645664.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2094"/>
    <dbReference type="EnsemblBacteria" id="ADZ10313">
        <property type="protein sequence ID" value="ADZ10313"/>
        <property type="gene ID" value="Metbo_2094"/>
    </dbReference>
    <dbReference type="GeneID" id="10278557"/>
    <dbReference type="KEGG" id="mel:Metbo_2094"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_0_2"/>
    <dbReference type="OMA" id="WDKQVHQ"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="104"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="159" mass="17678" checksum="527144DDE6B79106" modified="2011-05-03" version="1">MNVEDIMQKEVIKFNELDKIVDVAQSFRDNKISGAPVVDENNHVVGVISEGDIMRLIEIHSPKINLILPAPLDLIELPIKMKYELDEVAEDMQKAGSTVIDQIMTKKIIKVKPDTSVIDAAKLLDSHKIKRLPVIDNDGKLVGIITRGDIIASMVRGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ9</accession>
    <name>F0TBZ9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645692.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2122"/>
    <dbReference type="EnsemblBacteria" id="ADZ10341">
        <property type="protein sequence ID" value="ADZ10341"/>
        <property type="gene ID" value="Metbo_2122"/>
    </dbReference>
    <dbReference type="GeneID" id="10278585"/>
    <dbReference type="KEGG" id="mel:Metbo_2122"/>
    <dbReference type="eggNOG" id="arCOG04263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081191_0_0_2"/>
    <dbReference type="OMA" id="MLGETVF"/>
    <dbReference type="OrthoDB" id="104702at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PTHR42282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="2">
        <location>
            <begin position="100"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="298" mass="32095" checksum="CD80892825F92A2E" modified="2011-05-03" version="1">MKCSVFAPSHITGFFEIKDNKEPLKKGSRGAGIALDKGVVTNLQLTEKDSPNGSNIEVTINGEKDQKNTTITHKTMEIMEAKYKINHLLHGTQLKIDHKVDVPIGAGFGISAACAIGTALGITKLLQLNLTYNQATSIAHLAEIEMQSGLGDVVAEVNGGITLRIKEGAPGVGVLDKLLPKNKDLYILCKSLGCIETSEIIGDPGHKERINQTGGAMLNQLLLDPVPKTFLKLSKKFAENTKLMTPEISEIIAILQEETMGASMAMLGNTAFAISKTPETSIENVIVSRIDEQGCRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC06</accession>
    <name>F0TC06_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogen marker protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2129</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645699.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2129"/>
    <dbReference type="EnsemblBacteria" id="ADZ10348">
        <property type="protein sequence ID" value="ADZ10348"/>
        <property type="gene ID" value="Metbo_2129"/>
    </dbReference>
    <dbReference type="GeneID" id="10278592"/>
    <dbReference type="KEGG" id="mel:Metbo_2129"/>
    <dbReference type="eggNOG" id="arCOG00854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086562_0_0_2"/>
    <dbReference type="OMA" id="GVDEGWT"/>
    <dbReference type="OrthoDB" id="93216at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016764">
        <property type="entry name" value="MeTrfase_MtxX_xsu"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019709">
        <property type="entry name" value="Methyltransf_MtxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03270">
        <property type="entry name" value="methan_mark_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="26238" checksum="E23A8CB1517A0203" modified="2011-05-03" version="1">MVVVAGVGKNKKILEAVADVDFDVVTAESEDELVELLMNGDVDAAVRGSLSASLIMSKLKQIYPELYRASYIEFNAKKFLLAPVGIDEGETVAQKLQIAVLASKFLEAEGVKPHIAVLSSGRPNDRGRNKRVDESMDAGEELTALIKEKSIDVKHYYALLEDAVKSDANIIIATDGIVGNTIFRTLVLVARAKSYGAVTLGMKEIYIDTSRSQDNNGYKRALTLADRLAKITQTRNSTNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC09</accession>
    <name>F0TC09_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chloramphenicol O-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.28" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645702.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2132"/>
    <dbReference type="EnsemblBacteria" id="ADZ10351">
        <property type="protein sequence ID" value="ADZ10351"/>
        <property type="gene ID" value="Metbo_2132"/>
    </dbReference>
    <dbReference type="GeneID" id="25394756"/>
    <dbReference type="KEGG" id="mel:Metbo_2132"/>
    <dbReference type="eggNOG" id="arCOG01854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975251_0_0_2"/>
    <dbReference type="OrthoDB" id="93082at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008811">
        <property type="term" value="F:chloramphenicol O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="285" mass="32473" checksum="2910342203039546" modified="2011-05-03" version="1">MVNIINRFKNHLLNRSGQYRFYKSQYEQIYEENSSNPEKMVKFETLSKQKDNDSILLKKLDELESLLKKEDNNQILLKKLGELKSEINPHKGEVFRNKRGKKLSDIRSSHPANSIKNVTVGDFTYGNPKIERFNENDTLIIGKFCSISKEVRILIGKGHNIQSASSYPFSSFLGTRRVNISKIPMMQCGDTIIGNDVWIGYGALILSGVTVGDGAVIGAGAVVSKDVEPYSIVVGSPLKHVRYRFPKEVRDLLVEKKWWDLPDEKIDELAPYLIDIKIEDLLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC71</accession>
    <name>F0TC71_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0975</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644573.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0975"/>
    <dbReference type="EnsemblBacteria" id="ADZ09222">
        <property type="protein sequence ID" value="ADZ09222"/>
        <property type="gene ID" value="Metbo_0975"/>
    </dbReference>
    <dbReference type="GeneID" id="10277424"/>
    <dbReference type="KEGG" id="mel:Metbo_0975"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_6_2_2"/>
    <dbReference type="OMA" id="RTQYLIP"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="328"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="479" mass="53951" checksum="EE072C8E8E0FFF6E" modified="2011-05-03" version="1">MGSERTLAKNTTVLLVANLMSYLLGFFTTLYTARYLGVEGFGILSLALSITGIFGVLTDFGLATLTIREVSRDKSLADKYIGNTAVMKLFLSLLTFGVIGLLVYIVGYPQTVANVIYIITLSVIMTAFTGIFNSIFQAYEKMEYLSLTIVLNAVIMIAGILLVIYRGLDIVVLSSVYFFSSLLVLIATFIIFSKRFFMPSIQLDFSFWKLTLQESFYFGVSSILVVIYFYIDSFMLSIMVNNSAVGIYNAAYKLIFVLMFLPNVFLISIFPLMSQHYESNRELLKVEYEKSVKYLFAIAMFFFVFVLLFADKIIYIIYGSGYDASIFALQALIFVVPVIFITYLFGNILGAINKQRILLIVTAINAIINVALNLVLIPHYSYVGASVATVITEVTGFSLMIIYISKYFSSISWVNNFLKTITVSVLVLTVTYFLRLNINWIIALVAGVVLFLVLMIVTRIISREDLEIFKRIIKKDSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA7</accession>
    <name>F0TCA7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10374">
        <property type="protein sequence ID" value="ADZ10374"/>
        <property type="gene ID" value="Metbo_2159"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2159"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="4">
        <location>
            <begin position="864"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="970" mass="104882" checksum="BA8F52880995BE22" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC4</accession>
    <name>F0TCC4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645742.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2176"/>
    <dbReference type="EnsemblBacteria" id="ADZ10391">
        <property type="protein sequence ID" value="ADZ10391"/>
        <property type="gene ID" value="Metbo_2176"/>
    </dbReference>
    <dbReference type="GeneID" id="10278639"/>
    <dbReference type="KEGG" id="mel:Metbo_2176"/>
    <dbReference type="eggNOG" id="arCOG00016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065902_0_0_2"/>
    <dbReference type="OMA" id="CCIGHIT"/>
    <dbReference type="OrthoDB" id="98664at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PfkB" evidence="1">
        <location>
            <begin position="161"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="301" mass="34012" checksum="EDD7B6908A69CE69" modified="2011-05-03" version="1">MARFMIIGPVTRDTINRDGMIYHHTGGAVYFQSAVFSSFNKDVTAVIPLSKSDTDLLKAFPEDAHILPVFVEETMEFENIYPNHDLNHRVQRARVPDNPVLPENLPESMESYDAAILCPLSPTDIPIETVKHIAKHDVPIYMGAQGYLRHLNADEIILKPWEDFPKFLKHVELLFLDEAEAGIITDFKDHDLETIARQLSKFGPKEIIITRGDRGALIYSSRSNELYRIHAFPPKQTMDPTGLGDTYMAAYASKKIEGYSSKSCGIFASMVSTMKLETIGVFKGNNQKVLERLKEHEITDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCC9</accession>
    <name>F0TCC9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2181</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645747.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2181"/>
    <dbReference type="EnsemblBacteria" id="ADZ10396">
        <property type="protein sequence ID" value="ADZ10396"/>
        <property type="gene ID" value="Metbo_2181"/>
    </dbReference>
    <dbReference type="GeneID" id="10278644"/>
    <dbReference type="KEGG" id="mel:Metbo_2181"/>
    <dbReference type="eggNOG" id="arCOG03247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107897_1_0_2"/>
    <dbReference type="OMA" id="YINRGKM"/>
    <dbReference type="OrthoDB" id="61656at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="18412" checksum="AC6497D50BE2B52B" modified="2011-05-03" version="1">MLITTSRKPSQRTRSFCKSLVRVLNSSYTNRGKMSLRDVLIKSSEMGYDKTAVISQMKANPSRIDFHQEDGELILSLDITVGLDNNSGSKSRVKLKDLKFYSELNELEGLRDILNIPSLTKGSEFKNLMLLKASEEPNTSKIEFYADDGSLTGPKIFIQGWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI9</accession>
    <name>F0TCI9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredox_DsbH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644617.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1019"/>
    <dbReference type="EnsemblBacteria" id="ADZ09266">
        <property type="protein sequence ID" value="ADZ09266"/>
        <property type="gene ID" value="Metbo_1019"/>
    </dbReference>
    <dbReference type="GeneID" id="10277468"/>
    <dbReference type="KEGG" id="mel:Metbo_1019"/>
    <dbReference type="eggNOG" id="arCOG02007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_1_2"/>
    <dbReference type="OMA" id="PFYFGTY"/>
    <dbReference type="OrthoDB" id="12642at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="DUF255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="PTHR42899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredox_DsbH" evidence="1">
        <location>
            <begin position="14"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="702" mass="80837" checksum="BAD7E250DCBDECEB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN8</accession>
    <name>F0TCN8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="7">2.7.8.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2214"/>
    <dbReference type="EnsemblBacteria" id="ADZ10428">
        <property type="protein sequence ID" value="ADZ10428"/>
        <property type="gene ID" value="Metbo_2214"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2214"/>
    <dbReference type="eggNOG" id="arCOG03199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_1_2"/>
    <dbReference type="OMA" id="HSFELHG"/>
    <dbReference type="OrthoDB" id="88899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="357" mass="38096" checksum="BC95B1F4074E030C" modified="2011-05-03" version="1">MNNFNLEIFIFLLAFLSTAIFTYLVKKFLKYADVKDNPIVTEHSHKAGTPTMGGIAILMGVLLIACVYYTNSQLVLTVLIMMVAGIVGLMDDLIGLKTKEIQKVVMNKSAEPVEIGRLTLKNGENARVTTPKAKKDLEELLRTDKVEIIGEAPIKSEIKENEKIASQILVSLFLVASGAVGASVLGFNIGLLIIPVVICGIVGSINSVNLIDGMDGLAAGILGIASIASGLFAFSKGNPMGALPFIVIAGTCFGFLVFNRYPASIFMGDTGSFALGAGYITAAFLGDVVYFAIIALAVPIISVIISLLHRAHVIKLPVEPLHHTLHYKGLSEKKIVAVYWGATIVISGAALFIYNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ0</accession>
    <name>F0TCQ0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal-protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645791.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2226"/>
    <dbReference type="EnsemblBacteria" id="ADZ10440">
        <property type="protein sequence ID" value="ADZ10440"/>
        <property type="gene ID" value="Metbo_2226"/>
    </dbReference>
    <dbReference type="GeneID" id="10278692"/>
    <dbReference type="KEGG" id="mel:Metbo_2226"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OMA" id="YMNFYEM"/>
    <dbReference type="OrthoDB" id="95090at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="16862" checksum="433380AFB08BEC3C" modified="2011-05-03" version="1">MIIREFKRQDIKRVLEIEKASFNDPYPPNILIDIYNLGAGFLVAQENNRIVGYIIFWIKYEDEGHIISIAVDKNFRRLEVGTKLVEASIESFKKFSVKILKLEVRVGNTGARKFYSKIGFKEYKVVEDYYEDLEDAVIMTKPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ1</accession>
    <name>F0TCQ1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carA</name>
        <name evidence="3" type="ordered locus">Metbo_2227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645792.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2227"/>
    <dbReference type="EnsemblBacteria" id="ADZ10441">
        <property type="protein sequence ID" value="ADZ10441"/>
        <property type="gene ID" value="Metbo_2227"/>
    </dbReference>
    <dbReference type="GeneID" id="10278693"/>
    <dbReference type="KEGG" id="mel:Metbo_2227"/>
    <dbReference type="eggNOG" id="arCOG00064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_2"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="OrthoDB" id="35557at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="2">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="360" mass="39912" checksum="1C4B32ED4C0E4E9E" modified="2011-05-03" version="1">MLKEAKLALEDGTILKGEGFGFETIKTGEVVFATGMTGYVESLTDPSYKGQILMSTYPLQGNYGISREWFQSEGIKAEGFIVREQNNYPSHRLSQENLNDFLEEFEVPGISGIDTRALTLKIREHGAMKGAISTEEIEDDELLKLTKEQTEIQNMDLVDKVSVKKPTIIGEDNKHRAVILDCGIKKNSINALLKRDIGVVLVPYKTGYKEIMDYDPEAVLVSSGPGNPTRVTEAAQTVLDLSTKLPIFGICLGQQIISLAFGAKINKMKFGHRGINQPVKDLKTGKVSITSQNHGFTLDPNSCKDIPINITQINLNDHTPEGLEHEELPIVSVQYHPEAGPGPHDSDSYFDRFLETLKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ9</accession>
    <name>F0TCQ9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TfuA-like core domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645800.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2235"/>
    <dbReference type="EnsemblBacteria" id="ADZ10449">
        <property type="protein sequence ID" value="ADZ10449"/>
        <property type="gene ID" value="Metbo_2235"/>
    </dbReference>
    <dbReference type="GeneID" id="10278701"/>
    <dbReference type="KEGG" id="mel:Metbo_2235"/>
    <dbReference type="eggNOG" id="arCOG05002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090311_0_0_2"/>
    <dbReference type="OMA" id="MISFREM"/>
    <dbReference type="OrthoDB" id="72543at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012924">
        <property type="entry name" value="TfuA_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07812">
        <property type="entry name" value="TfuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TfuA" evidence="1">
        <location>
            <begin position="52"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="215" mass="23736" checksum="669351583332E704" modified="2011-05-03" version="1">MVTNKKIIIFTGPSLQPKEAETILKADYRPPIARGDIIQALTDEPDIIGIIDGVFHKEPAVSHKEILEALKKDVTVVGGASMGALRASELDDFGMVGVGQVYMDYKTGVIESDDDVAVVINPDTMEQLSEALISINYTFKAALKQGVINRSDYDLLIKTAKSIYYPKRSYSKVFAESGLEKSKVELLQKFLEVHAIDVKRMDALAVLDYIKKIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR6</accession>
    <name>F0TCR6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin synthesis protein P47K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645807.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2242"/>
    <dbReference type="EnsemblBacteria" id="ADZ10456">
        <property type="protein sequence ID" value="ADZ10456"/>
        <property type="gene ID" value="Metbo_2242"/>
    </dbReference>
    <dbReference type="GeneID" id="10278708"/>
    <dbReference type="KEGG" id="mel:Metbo_2242"/>
    <dbReference type="eggNOG" id="arCOG01231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1193419_0_0_2"/>
    <dbReference type="OMA" id="DMCPDHF"/>
    <dbReference type="OrthoDB" id="56639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="3"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="25659" checksum="99D8E32214C35D01" modified="2011-05-03" version="1">MRMIIVAGTPGSGKTAVLIHALRSLKERDLKSSVVKIDCLYTDDGKKFEKIGIPTKVGLSMDMCPDHFAIYNIEDMIEWAQENDSECLVIETAGLCHRCAPYTQNSLGVCVIDATSGPNTPLKVGPFLSTADIAVITKGDMISQAEREIFRERVLEVNPNCKVIDANGLSGQGCMELAEEIYQTEEVSLEEEKLRHSAPLAVCTLCVGETKVNKKHHRGILRKIDGFQTYKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T1</accession>
    <name>F0T5T1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1079"/>
    <dbReference type="EnsemblBacteria" id="ADZ09324">
        <property type="protein sequence ID" value="ADZ09324"/>
        <property type="gene ID" value="Metbo_1079"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1079"/>
    <dbReference type="eggNOG" id="arCOG02386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="11906" checksum="ACF130CB53FD281D" modified="2011-05-03" version="1">MLSLDDTHNYDLVHSKRLDDGIKMFIKEKFDVMLLDLGLPDSDGMETFDIMRYNAPDIPIIVLTGFKEDVIEMGAVGRGAKNYIVKDELNTKLLIKSIDDALEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U0</accession>
    <name>F0T5U0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Chorismate synthase</fullName>
            <shortName evidence="8">CS</shortName>
            <ecNumber evidence="4 8 9">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroC</name>
        <name evidence="10" type="ordered locus">Metbo_1088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="8 9">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644684.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1088"/>
    <dbReference type="EnsemblBacteria" id="ADZ09333">
        <property type="protein sequence ID" value="ADZ09333"/>
        <property type="gene ID" value="Metbo_1088"/>
    </dbReference>
    <dbReference type="GeneID" id="10277537"/>
    <dbReference type="KEGG" id="mel:Metbo_1088"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_2_2"/>
    <dbReference type="OMA" id="MLSINAV"/>
    <dbReference type="OrthoDB" id="50229at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="7 8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="300"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="363" mass="38474" checksum="88DF092934CC2E23" modified="2011-05-03" version="1">MSGNSIGKIFTVTTFGSSHGTALGAVIDGCPAGLELSTQDIQTELDKRRPGTSKITTSRNEMDQVKLVSGIFEGKTDGTPITALVYNSDMDSSAYENIKDTPRPGHGDYTWKARYDVYDYRGGGRGSGRVTIGHVIGGAVAKKLLNTLNIKVISHVTQVGTIKADPVDVNELETNINQNAVRCADLEAAERMEAEIIETKKSGDSIGGIVETVVLNPPAGLGDPIFGKLDAELAGGLMGIGSVKGVEIGLGFKSAELKGSQMNDEFLIETNTIKTRTNHSGGILGGITTGMPINIRMAVKPTPSISLTQKTVDTKSMESKDILIQGRHDPCICPRVTTVAEATVSIILADQLIRAGYIDPCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V1</accession>
    <name>F0T5V1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644695.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1099"/>
    <dbReference type="EnsemblBacteria" id="ADZ09344">
        <property type="protein sequence ID" value="ADZ09344"/>
        <property type="gene ID" value="Metbo_1099"/>
    </dbReference>
    <dbReference type="GeneID" id="10277549"/>
    <dbReference type="KEGG" id="mel:Metbo_1099"/>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_4_2"/>
    <dbReference type="OMA" id="AYADEEM"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041359">
        <property type="entry name" value="MetOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18546">
        <property type="entry name" value="MetOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="284" mass="32589" checksum="105C6219AC861F9C" modified="2011-05-03" version="1">MSNERIMIVEDESIVAMGIKHKLEDLGYDVVGIVATGEGAVTTALKTVPDLILMDIVLKGDLDGIEAAQQIHKHQDTPIIYITAYSDEEVLERARITEPYGYILKPFKKSEINANIQMAIYKHKKDRKRSEETKKRVLADFYDFILTAMPTCSTDSDMEMRQLLLRVFAERLDDDLYPKFVEELNPDEIEDADELFETYVAWLANLFGDFGIKTIVTEEGDRIYLEFLNCPWPEDAKKKPIFCLNCQAMINRSFNWTGLRGSIERRSSITDGSESCVFKFNKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W0</accession>
    <name>F0T5W0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">VRR-NUC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644704.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1108"/>
    <dbReference type="EnsemblBacteria" id="ADZ09353">
        <property type="protein sequence ID" value="ADZ09353"/>
        <property type="gene ID" value="Metbo_1108"/>
    </dbReference>
    <dbReference type="GeneID" id="10277558"/>
    <dbReference type="KEGG" id="mel:Metbo_1108"/>
    <dbReference type="HOGENOM" id="CLU_1264577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
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        <property type="entry name" value="VRR_NUC"/>
    </dbReference>
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        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
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    <feature type="domain" description="VRR-NUC" evidence="4">
        <location>
            <begin position="115"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="26292" checksum="17480DB9B4E527D9" modified="2011-05-03" version="1">MISLTNINLTREKSPRRGKAKYLLGNGIWGLTAEESAKDYYESLGYKARNTENQFWQSFMIFFLWDNKIEGKISVNEKIDYLKSKNLENYLIQSHNEHFINTFDGNYKYSRYTDRWDHYLIEDYLIPLKFIEKEKLLEFIKRLAFKNTGLPDLIVYNEENFFFSEVKSENDRISDDQFEWHMFLSEKLNLNVEILLINNSQKKIENIKNRYQKCGFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W2</accession>
    <name>F0T5W2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIR protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644706.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1110"/>
    <dbReference type="EnsemblBacteria" id="ADZ09355">
        <property type="protein sequence ID" value="ADZ09355"/>
        <property type="gene ID" value="Metbo_1110"/>
    </dbReference>
    <dbReference type="GeneID" id="24964531"/>
    <dbReference type="KEGG" id="mel:Metbo_1110"/>
    <dbReference type="eggNOG" id="arCOG09612">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_885100_0_0_2"/>
    <dbReference type="OrthoDB" id="131482at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="320" mass="36876" checksum="284BA766CBD70A45" modified="2011-05-03" version="1">MNKHVFISYSHEDKYFANRIAEDLMKNGIEVWFDKWEIQVGDSLIEKIFSEGLSNSDYFLILLSKDSINSKWVKEELNNAIIRRMEGVTKLIPLLTESIEIPQPLKALKWLDMYSNYDKGLRSLINTIYNISEKPPVGKIPEHIVSLRNSVGGLSRQASTIGSLLIFKQEEEPGFERSYSASELHSLIEFMNNEEFNDAIDELEEYGLVKTLKVFGMHPYDFGQVNGTYALYLHFKNELKYDPENDIKSVAAVVSEKKEIDGNKLHSMLNIPPVRLNYAVAYLEDYGYVKVLKFLGTHPYDFGIVTATRHTRQFVDEHCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y6</accession>
    <name>F0T5Y6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644730.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1135"/>
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        <property type="gene ID" value="Metbo_1135"/>
    </dbReference>
    <dbReference type="GeneID" id="10277585"/>
    <dbReference type="KEGG" id="mel:Metbo_1135"/>
    <dbReference type="HOGENOM" id="CLU_2032883_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="14287" checksum="BF9A5BA064017619" modified="2011-05-03" version="1">MDRIGEQSRLSELPKPKYKTKPPTLLLNWDQTRPDLKKFIYLHIIRAAGEYQDPHMKLTASKLVESMASNTGIIKRQLNLYIENLLFYGYLLKPYLNDKIIVSDKLYNDLRNLDRIPEEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T624</accession>
    <name>F0T624_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2317</name>
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    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645882.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10531">
        <property type="protein sequence ID" value="ADZ10531"/>
        <property type="gene ID" value="Metbo_2317"/>
    </dbReference>
    <dbReference type="GeneID" id="10278783"/>
    <dbReference type="KEGG" id="mel:Metbo_2317"/>
    <dbReference type="eggNOG" id="arCOG09465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369540_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="199" mass="22826" checksum="E1DA883B718BD3E9" modified="2011-05-03" version="1">MNISPIEKRLIHVTAFLIVVFIFSELYNTHLIPLSYGIFFIIIITFLGFILQYYIKVDESKEHTKLILWESLIIILLIGMIVNPFYISDSLEDNTQNIFQNSCSDISYYELNKNFNNYEGKKVKFSGFVEHTDVEGDSKLIILDVNKEPNDKIAVFHTGDLSINANNSITVYGIITGKRELSDPGKFYPTITAFYINST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T638</accession>
    <name>F0T638_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645896.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2332"/>
    <dbReference type="EnsemblBacteria" id="ADZ10545">
        <property type="protein sequence ID" value="ADZ10545"/>
        <property type="gene ID" value="Metbo_2332"/>
    </dbReference>
    <dbReference type="GeneID" id="10278798"/>
    <dbReference type="KEGG" id="mel:Metbo_2332"/>
    <dbReference type="eggNOG" id="arCOG04140">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179754_1_0_2"/>
    <dbReference type="OMA" id="VVHSIDM"/>
    <dbReference type="OrthoDB" id="126791at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="PTHR42240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="SSF160363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10623" checksum="5ECD17F91A8443DF" modified="2011-05-03" version="1">MAKGLIRIVLDILKPHEPIIPTFAKYLSEINGVEGVNITLLEIDKETENIKVTMQGDDLDYDVITEAIEEYGGSIHSVDEVVAGKKLIEEVTTPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A2</accession>
    <name>F0T6A2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1173</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644768.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1173"/>
    <dbReference type="EnsemblBacteria" id="ADZ09417">
        <property type="protein sequence ID" value="ADZ09417"/>
        <property type="gene ID" value="Metbo_1173"/>
    </dbReference>
    <dbReference type="GeneID" id="10277623"/>
    <dbReference type="KEGG" id="mel:Metbo_1173"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="OrthoDB" id="102435at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="10">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine_dh" evidence="9">
        <location>
            <begin position="157"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
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        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="339" mass="36092" checksum="6B59EE3F94328A7F" modified="2011-05-03" version="1">MKLCILGFGAVGMGVVKVLSMKNDMLIDKYGLDISVVAVTDRSGAAVNPEGLDLQLLIETKENTNKISDYPEYGVPGLDSAEVVETVEFDCLVEVTPTNIDHGEPANTHMMETLKKGRDVVTSNKGPLSLYFSELVETANSNNAEFKYEASVGGAMPIINFAHNNLAGNNISSVLGILNGTTNYILSRMANEGSSYEQTLKEAQELGIAETDPTQDVEGIDAAAKIVILANSVLNRNVTIKDVHMEGITNITPESIELAKKEGFLIKLIGEASNNNLEVAPRLVKEGSPFAVDGTLNVATVMTDLADDVTVVGKGAGSIETASAILSDIICVKKSRNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B2</accession>
    <name>F0T6B2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644778.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1183"/>
    <dbReference type="EnsemblBacteria" id="ADZ09427">
        <property type="protein sequence ID" value="ADZ09427"/>
        <property type="gene ID" value="Metbo_1183"/>
    </dbReference>
    <dbReference type="GeneID" id="10277633"/>
    <dbReference type="KEGG" id="mel:Metbo_1183"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_3_2_2"/>
    <dbReference type="OMA" id="QFLIFDI"/>
    <dbReference type="OrthoDB" id="105513at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
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        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="131" mass="14069" checksum="EAFD21AEB1227D12" modified="2011-05-03" version="1">MPLRVAVASSDGKFINQHFGHAEQFLIFDIDDEGNYEYIELRKNEPTCSGGNHTAGSMKNAVGVLDGVKVVLVAQIGPGASQNLLLSGIQSFSVPSYIDEALEKLGSSYAQKLKASKSSSDQDTKNQGDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B3</accession>
    <name>F0T6B3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="2">4.4.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.4.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644779.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1184"/>
    <dbReference type="EnsemblBacteria" id="ADZ09428">
        <property type="protein sequence ID" value="ADZ09428"/>
        <property type="gene ID" value="Metbo_1184"/>
    </dbReference>
    <dbReference type="GeneID" id="10277634"/>
    <dbReference type="KEGG" id="mel:Metbo_1184"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_15_0_2"/>
    <dbReference type="OMA" id="RINIGCP"/>
    <dbReference type="OrthoDB" id="69863at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004121">
        <property type="term" value="F:cystathionine beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027619">
        <property type="entry name" value="C_S_lyase_PatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04350">
        <property type="entry name" value="C_S_lyase_PatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="42"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="400" mass="45645" checksum="A683D24DBF4B6CE5" modified="2011-05-03" version="1">MNYDLDKVYNRKNTDSLKWDFMKPIIGRDDIIPLWVADMDFPVAEPIKEAIKTRAEHPFYGYTQPGGSVVDSVVQRLKKKYNWNIEPEWVVFTPGVVPALQTAVTSLTHPGDEVILQQPSYHPFFPVVKNSGCSIVNNQLKLVNGRYVMDCEDLESKFHPKTRRLMGPGRLKTIIFCNPHNPVGRVWNPDEIEKMGNIVIDNGGVVISDEIHCEILFKGNKHTPFASISDKFEQNSIICMSPSKTFNLAGLEVSNIIIPNQRIREDFINTRAGMVPNPNLFGYTALKAAYKYGDEWLEQVLDYLQGNLDYIRKFLETNIPEIKMIEPQGTYLLWLDCRELGMDPHTLQSFMIDDAKVGMEDGYIFGESGQGFMRMNIATQRSVINSALNRIKNAYLLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G4</accession>
    <name>F0T6G4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP dependent DNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2385"/>
    <dbReference type="EnsemblBacteria" id="ADZ10598">
        <property type="protein sequence ID" value="ADZ10598"/>
        <property type="gene ID" value="Metbo_2385"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2385"/>
    <dbReference type="eggNOG" id="arCOG01347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_4_0_2"/>
    <dbReference type="OMA" id="AGFRWHK"/>
    <dbReference type="OrthoDB" id="51467at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="2">
        <location>
            <begin position="101"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="301" mass="34743" checksum="89FDD33659386F58" modified="2011-05-03" version="1">MNMIEPMLATLTDPSSLDPTDSWIIEPKYDGERIIAMNYGGKIVLWTRRNIQATHKFPEIVEALNKNIHDDNWIVDGEMTVKGGFRQLLNRNVEDRFKISLLSKKIPATYNIFDIVCYNKQDISKKNLYERKSVLLHSVDEDERIKIVQFDVLKDPEKQFNQYITDGYEGAVIKNLDSNYEFGQRSKNWLKVKKGDTVDVHIIGATKSASSIPFGALMMEKDGKYFGKVGTGFSDKEREEILRMLMENQQPLTIDIPADLESEVLVTSKPFPAEIKMQEMIKRSPRAPVWVRFRWDDVSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I4</accession>
    <name>F0T6I4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sucC</name>
        <name evidence="9" type="ordered locus">Metbo_2405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645969.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2405"/>
    <dbReference type="EnsemblBacteria" id="ADZ10618">
        <property type="protein sequence ID" value="ADZ10618"/>
        <property type="gene ID" value="Metbo_2405"/>
    </dbReference>
    <dbReference type="GeneID" id="10278877"/>
    <dbReference type="KEGG" id="mel:Metbo_2405"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="72275at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="9"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
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        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
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        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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            <position position="245"/>
        </location>
        <ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="368" mass="39790" checksum="EF61693E42DD41B4" modified="2011-05-03" version="1">MKIHEYIAKEIFKAEGIPVPASQMVKNSEDAHKAALEINKPVAIKSQILVGGRGKAGGIQFATDPDEARDVSSKILGSTVKGELVEMVLVEEKLSIESEFYVSVVLDRSAKKPLIMASSSGGMEIEEVATETPEKIVKYYINSLDEFLPYQGREIAIKMGIPNELISKVGGIIWKLVQIFDKYDATIAEINPLVLTKEGIIAADAKLDIDDDALYRQKKLVELENIKSGEFAYVKLDGNIAVIGNGAGLTLSGMDMLKLYGGEPATFLDIGGGSSRENIAKALNIVISDPDVKTVFLNVLGGITRADDVARGVLDVMKESERKIPLVIRLTGTNEEEGQKILKDAGVSFETSMEAAAQKAVELCSTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J0</accession>
    <name>F0T6J0_METLA</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_2411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="Metbo_2411"/>
    </dbReference>
    <dbReference type="GeneID" id="10278883"/>
    <dbReference type="KEGG" id="mel:Metbo_2411"/>
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    <dbReference type="OrthoDB" id="107744at2157"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="10"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11860" checksum="2DA1085001C0BAE6" modified="2011-05-03" version="1">MKIAVASSNGKIIDLHFGDADRFIIFELKDGEGEFSEIREKNGMNIDNHQERWLASLDLVEDCKAVLCSKIGKEPSIELMKLGIKPIKLDCNVREAVKSCAEHLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N2</accession>
    <name>F0T6N2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme F390 synthetase</fullName>
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        <name evidence="2" type="ordered locus">Metbo_0012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0012"/>
    <dbReference type="EnsemblBacteria" id="ADZ08265">
        <property type="protein sequence ID" value="ADZ08265"/>
        <property type="gene ID" value="Metbo_0012"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0012"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_606379_0_0_2"/>
    <dbReference type="OMA" id="STEGTMC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017720">
        <property type="entry name" value="Coenzyme_F390_Synthase"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03335">
        <property type="entry name" value="F390_ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="81"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="446" mass="50979" checksum="AB8B68264F0810BE" modified="2011-05-03" version="1">MDYYNHVIETMKRPELDVLVENRIQHTIRYAYKHSPFYRKWFDRNGINPSDIHSHEDLRELPLISGGLIRENQPPITPGFEFKSIPWNDVYTIHETSGTSGVPKSFFLTWDDWQRYAEKYARAFVSQNFGEGDRVVICASYGMNVGANTMTLGAHKINMSIIPEGKCSFPIRIIQNYQPTGIVGSVFKLLRLAKRMKSSGLDPKESSIKRLIAGGESFPEESRKYLEELWDVPVYNTYGSTEGTMCGECRMKSGLHVPEDLVHLDVYDPQLKDFVNDGECGRIVLTTLLPEGGKTGNLLINYDTDDTTTVVSREKCSCGRTHMKIMNPQREAETVYVGGFPFNKVDVEQGVFQRDNMEYLTGEYEAFLYGDEEETTLRVSMECENSPNCDVGSIENNFLETFLKFKPGLRQAHSDGTFNIIFNFTEPEGLEFYKISGRPKRLVDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R2</accession>
    <name>F0T6R2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643646.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0042"/>
    <dbReference type="EnsemblBacteria" id="ADZ08295">
        <property type="protein sequence ID" value="ADZ08295"/>
        <property type="gene ID" value="Metbo_0042"/>
    </dbReference>
    <dbReference type="GeneID" id="10276464"/>
    <dbReference type="KEGG" id="mel:Metbo_0042"/>
    <dbReference type="eggNOG" id="arCOG07501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093776_0_1_2"/>
    <dbReference type="OMA" id="KMSIHAG"/>
    <dbReference type="OrthoDB" id="96272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
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        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="22636" checksum="7FEBA7A4C78A533D" modified="2011-05-03" version="1">MDNIQNNHPSELETAKFISMVAHPPVISIPTFIILNYFLAGPDQFIVPTIISIIFGALIPISTSLILIKKMHTDLDITDRTKRTVPLIFAICSYLLGFLMLLWYGAPDIVSVLMLIYGTNTLLILIINFYWKISIHAMGIAGPTAAFIFTFGPLGIIVGLIIPLVMWSRLKLKKHTPSQVIAGSLLGLLSTWIQLSYLVPLIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U2</accession>
    <name>F0T6U2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin (Vitamin B12) biosynthesis CbiM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0072</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643676.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0072"/>
    <dbReference type="EnsemblBacteria" id="ADZ08325">
        <property type="protein sequence ID" value="ADZ08325"/>
        <property type="gene ID" value="Metbo_0072"/>
    </dbReference>
    <dbReference type="GeneID" id="10276495"/>
    <dbReference type="KEGG" id="mel:Metbo_0072"/>
    <dbReference type="eggNOG" id="arCOG02248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052508_2_1_2"/>
    <dbReference type="OMA" id="PWGTSGH"/>
    <dbReference type="OrthoDB" id="92958at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="24051" checksum="2911EA51C360DEAC" modified="2011-05-03" version="1">MHIPDGFIPLWQCAIYYVILIVALYFSQKWARKNLDEKMVPLMAVLAAGIFAIMSMNMPIPFGTSGHMVGGALVAIVFCAPEAAIIVFTLVLLVQGIFFGDGGVTALGANVLNMGIIGGFTGLYVFKALRKPIGKYPAIAIASWLSIFLAAEAVAVEMWLAGTFPLGAGLIFMGLYHAFIGIIEAVLTVVVIMALEKLRPDLLGWNRKRRVEDIDTETGEVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V6</accession>
    <name>F0T6V6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intracellular protease, PfpI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1234"/>
    <dbReference type="MEROPS" id="C56.001"/>
    <dbReference type="EnsemblBacteria" id="ADZ09476">
        <property type="protein sequence ID" value="ADZ09476"/>
        <property type="gene ID" value="Metbo_1234"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1234"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_2"/>
    <dbReference type="OMA" id="LCHGPWV"/>
    <dbReference type="OrthoDB" id="98884at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="PTHR42733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="18693" checksum="EEFBD98126629DA9" modified="2011-05-03" version="1">MIGILIDEMFEDVEYIKPSEAFVNEGHSIVHIGIKEGQVVKGKRKSTPVTIEESVSKYNIDDLDALMIPGGCSPDHLRGYESAVNFVKNFFKTGKPIFAICHAPQILITADLLHEKKVTGWRSIVQDLKNAGAIYIDNEIVVDDNLISSRGPKDIPVFIDTALNVLKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T729</accession>
    <name>F0T729_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyruvate ferredoxin/flavodoxin oxidoreductase, beta subunit</fullName>
            <ecNumber evidence="6">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646014.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2450"/>
    <dbReference type="EnsemblBacteria" id="ADZ10663">
        <property type="protein sequence ID" value="ADZ10663"/>
        <property type="gene ID" value="Metbo_2450"/>
    </dbReference>
    <dbReference type="GeneID" id="10278922"/>
    <dbReference type="KEGG" id="mel:Metbo_2450"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OMA" id="EHDGFAH"/>
    <dbReference type="OrthoDB" id="43666at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="53"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="PFO_beta_C" evidence="5">
        <location>
            <begin position="199"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="31961" checksum="2CD3422CDE7D492B" modified="2011-05-03" version="1">MDSTQFDVEGADVAWCPGCGNFSILKALKETLAELEIAPEEVVMVSGIGQAGKLPHYIKSNVFNGLHGRSLPISAAIRAVNPEILVVNVSGDGCTYGEGGNHFIHNIRRNPNIVNIVHNNMVYGLTKGQASPTSQLDFNTPLQVDGVFEEPFNPLATAISLNASFVARAFSGDINQTKAILKKAIDHNGYSLVDIFQPCVTFNKLNTFQWFKNNTYYLDESHDPTDRELAFKRAIETEKYPLGIFYINEKKKTFEESLQVYKTDKSPMFTRKQDMDKLKNLMESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T740</accession>
    <name>F0T740_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="11">5.4.2.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2461</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2461"/>
    <dbReference type="EnsemblBacteria" id="ADZ10674">
        <property type="protein sequence ID" value="ADZ10674"/>
        <property type="gene ID" value="Metbo_2461"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2461"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OMA" id="PHNPEPL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="151"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="257"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="372"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="450" mass="49103" checksum="C96ECEA8339F37B4" modified="2011-05-03" version="1">MTIKRLFGTFGVRRIANQELTPEFASRLAAAYGSIVKGTVAVGGDPRTSTEMIKHAVIAGLLSAGCKVIDLGILPTPAVQFAVRNYYDGGVMITASHNPPKYNGLKFVDSDGIGIEEKWEQKIEAMFFDEEPVRVSWNAIEESTKNHGIVEEYIENVIERVDSQAIKEANLKVIVDCGSGAACFTTPYLLRRLGCQVTTMNCQPDGFFPGRNPEPTSENLEELKNVVKATGADLGIAHDGDADRTICIDENGEFVFGDKTFALVEKAMLKENGGGTIVTTVATSAAIYDIAEEYNGEVIATRVGDLLVARELKDQNGLFGGEENGGLIFPEFVYGRDAALSTAKIVEIMAKEAKPLSELIQELPKYCSAKLKVECADHQKSTVMDRIVEATSDHEVDTKDGVKILTEKGWVIIRPSGTEPIFRCFAEAQTEEDALKMAKWGISLVEENME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T793</accession>
    <name>F0T793_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helix-turn-helix domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646078.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2514"/>
    <dbReference type="EnsemblBacteria" id="ADZ10727">
        <property type="protein sequence ID" value="ADZ10727"/>
        <property type="gene ID" value="Metbo_2514"/>
    </dbReference>
    <dbReference type="GeneID" id="10278988"/>
    <dbReference type="KEGG" id="mel:Metbo_2514"/>
    <dbReference type="eggNOG" id="arCOG04891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145181_0_0_2"/>
    <dbReference type="OMA" id="LCASCIF"/>
    <dbReference type="OrthoDB" id="91206at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="120"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="16416" checksum="6B461AAE24B8F922" modified="2011-05-03" version="1">MPKHIASGLKYLAALELVRDGYSQKQISKMLNVNKSTLSHYLNGRNISLGSIEIAEIVREFSPRDFLVFTDAIIEDKEATKTIVKTCLVNKYSVEVKDSCIGCGICVDTCLIDAIVLDDLKAHIDSDICCGCQICAEKCPTNSINILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A0</accession>
    <name>F0T7A0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643680.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0076"/>
    <dbReference type="EnsemblBacteria" id="ADZ08329">
        <property type="protein sequence ID" value="ADZ08329"/>
        <property type="gene ID" value="Metbo_0076"/>
    </dbReference>
    <dbReference type="GeneID" id="10276499"/>
    <dbReference type="KEGG" id="mel:Metbo_0076"/>
    <dbReference type="eggNOG" id="arCOG02587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="CYDVCPK"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="70" mass="7687" checksum="82ECAC3B7D1AFEA8" modified="2011-05-03" version="1">MKIIVDEDKCTGCGICKEACPKGAKIWDVKKKAMATNLEFCHLCTICAGKCPEGAIQVIRDEPNEKKDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I3</accession>
    <name>F0T7I3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644902.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1309"/>
    <dbReference type="EnsemblBacteria" id="ADZ09551">
        <property type="protein sequence ID" value="ADZ09551"/>
        <property type="gene ID" value="Metbo_1309"/>
    </dbReference>
    <dbReference type="GeneID" id="10277760"/>
    <dbReference type="KEGG" id="mel:Metbo_1309"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327286_0_0_2"/>
    <dbReference type="OrthoDB" id="141674at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="11104" checksum="B0C85225D7CB384F" modified="2011-05-03" version="1">MKFNELVNKEVIDQKGNVLGKVKNIEWDTESKEVINIEVSSGGFKDTLGMSEHQILSYDSIESIGDKVLLKKQIIEPVKTPAKEENEETTDNLDFYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K8</accession>
    <name>F0T7K8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644927.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1335"/>
    <dbReference type="EnsemblBacteria" id="ADZ09576">
        <property type="protein sequence ID" value="ADZ09576"/>
        <property type="gene ID" value="Metbo_1335"/>
    </dbReference>
    <dbReference type="GeneID" id="10277786"/>
    <dbReference type="KEGG" id="mel:Metbo_1335"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="OMA" id="HEISGCT"/>
    <dbReference type="OrthoDB" id="99888at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13421" checksum="64E6F3EFD784B0E9" modified="2011-05-03" version="1">MTITKKEKLDDVLSGLLKVGQIKACAVVSKEGLLINSRTPPDVDARIFSALCSTIMGAAEAASGQMTTGIVSQISLKTEKGTIVLIPAGQKAILTVLTEPEAQIGLIFYEMEQRAKMVDQILREM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L6</accession>
    <name>F0T7L6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="14">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">metG</name>
        <name evidence="18" type="ordered locus">Metbo_1344</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">AL-21</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="14"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644935.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1344"/>
    <dbReference type="EnsemblBacteria" id="ADZ09584">
        <property type="protein sequence ID" value="ADZ09584"/>
        <property type="gene ID" value="Metbo_1344"/>
    </dbReference>
    <dbReference type="GeneID" id="10277795"/>
    <dbReference type="KEGG" id="mel:Metbo_1344"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
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    <dbReference type="OrthoDB" id="6590at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:methionine-tRNA ligase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:tRNA binding"/>
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    </dbReference>
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        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Anticodon_Ia_Met"/>
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        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Anticodon_Met"/>
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        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
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        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
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        <property type="entry name" value="MetRS_core"/>
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        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF57770"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="17">
        <location>
            <begin position="562"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="14">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="14">
        <location>
            <begin position="326"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="658" mass="75411" checksum="AFF82F661474C60C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q2</accession>
    <name>F0T7Q2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Metbo_2521</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2521"/>
    <dbReference type="EnsemblBacteria" id="ADZ10734">
        <property type="protein sequence ID" value="ADZ10734"/>
        <property type="gene ID" value="Metbo_2521"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2521"/>
    <dbReference type="eggNOG" id="arCOG04358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_4_2_2"/>
    <dbReference type="OMA" id="RYCAGAT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Mo-bisPGD binding" evidence="3">
        <location>
            <begin position="233"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="3">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="27361" checksum="9523092BBE8B7BFA" modified="2011-05-03" version="1">MMEMFKEVKAVRFEGKPSEVIETIVNDEDIEITINNSVSRRFSISPNSLKEFTVGYLLGEGLAGSVDNITNINIDGKTIKAEIDLEDFDIRKELVVGSDCFGGWRTKIEFVGQVESEFSVESNDLIDAFGKLKKKAEVWQKTGGTHVAGLVSGDDFIAIEDVSRHVAVDKVIGAGAIAKFDFSKSFIVYSGRMPADMLIKIARVGIPIIASNAAPTSSGIAVADEARVTMVGFVRGKRFNIYTHPHRIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S2</accession>
    <name>F0T7S2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643760.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0157"/>
    <dbReference type="EnsemblBacteria" id="ADZ08409">
        <property type="protein sequence ID" value="ADZ08409"/>
        <property type="gene ID" value="Metbo_0157"/>
    </dbReference>
    <dbReference type="GeneID" id="10276583"/>
    <dbReference type="KEGG" id="mel:Metbo_0157"/>
    <dbReference type="eggNOG" id="arCOG09722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433870_0_0_2"/>
    <dbReference type="OMA" id="EIEMAIF"/>
    <dbReference type="OrthoDB" id="127452at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043962">
        <property type="entry name" value="DUF5750"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19024">
        <property type="entry name" value="DUF5750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="10549" checksum="85B7135BA6935D14" modified="2011-05-03" version="1">MLVEVYDFGNDAGDYYVVYRVKELSEEEQKKLKSKVEGTVEIKEGQLFITTHFEKKYFPFGSEAAKFRSDDFVAREEIEMTVYLTSLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W8</accession>
    <name>F0T7W8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0203</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643806.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0203"/>
    <dbReference type="EnsemblBacteria" id="ADZ08455">
        <property type="protein sequence ID" value="ADZ08455"/>
        <property type="gene ID" value="Metbo_0203"/>
    </dbReference>
    <dbReference type="GeneID" id="10276629"/>
    <dbReference type="KEGG" id="mel:Metbo_0203"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="OMA" id="MVKFCPK"/>
    <dbReference type="OrthoDB" id="121393at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="DNA-dir_RNA_pol_M/15kDasu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="PTHR11239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="TFIIS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12077" checksum="CBC20901335A567C" modified="2011-05-03" version="1">MEFCPKCGAVMFPKGDIFECRNCEHTTKATKESMSEYEISEKVESKDSIIVTSDEIQTLPTAVVKCPKCGNKEASWWLIQTRGADESETRFFRCTKCSLTWREYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X6</accession>
    <name>F0T7X6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643814.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0211"/>
    <dbReference type="EnsemblBacteria" id="ADZ08463">
        <property type="protein sequence ID" value="ADZ08463"/>
        <property type="gene ID" value="Metbo_0211"/>
    </dbReference>
    <dbReference type="GeneID" id="10276637"/>
    <dbReference type="KEGG" id="mel:Metbo_0211"/>
    <dbReference type="eggNOG" id="arCOG00348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="OMA" id="ICLMGET"/>
    <dbReference type="OrthoDB" id="64950at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="CHP00062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00162">
        <property type="entry name" value="TIGR00162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="28096" checksum="A08011EB757BDA86" modified="2011-05-03" version="1">MNKTFINNLEEVELSEPIFIEALPGIGHVGKLVAEHLIHELGAVKFAELYSPAFPPQVFVDEEGLIEPMKNEFYYLKSQGEAEQDYIILVGNTQGLSPEGQYEICGTIIDFVHNFGVKNIYTLGGLGTGQPVDKPKVFGAATNIELAEMLKGHDVTLRTADGGIIGASGLILGLGMSKGMSGVCLMGETPGYFIDAEASKAVLTILQKILNVDVDIAKLEERAEETRKMISKAQQMEREMSDRMNIVQGEEDLRYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y3</accession>
    <name>F0T7Y3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420 hydrogenase, subunit alpha</fullName>
            <ecNumber evidence="3">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643821.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0218"/>
    <dbReference type="EnsemblBacteria" id="ADZ08470">
        <property type="protein sequence ID" value="ADZ08470"/>
        <property type="gene ID" value="Metbo_0218"/>
    </dbReference>
    <dbReference type="GeneID" id="10276646"/>
    <dbReference type="KEGG" id="mel:Metbo_0218"/>
    <dbReference type="eggNOG" id="arCOG01549">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044556_1_0_2"/>
    <dbReference type="OMA" id="FMPDFTR"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="405" mass="44166" checksum="2BCF7206D91277C4" modified="2011-05-03" version="1">MSEKIVISPTSRQEGHAELVMDVDDEGIVTKGRYFSITPVRGLEKIVTGKVPETAPVIVQRICGVCPIPHTLASVEAIDDSLGIEIPKAARQLREMTLAAHTVNSHAIHHFLIATDFVPENLMATAINSVSEIRKTVQYVVDMVAGEGIHPADVRIGGMASNISETARKRLYARTKALIPKIDAHVDLMIGLIADKDLPDGLGVVNVPTLATHPLYGDRTKFDLDRFTEIMPETWYDDPEVGKRACSTIPLYDGRNVEVGPRARAAVFGSYNEKGVVAQHVARAMEMKTNVSKIVDLLAELDTSAPVMADYDVTGTNKLGIGAIEGPRGMDVHMAQIADGKTQFYSCLVPTTWNIPTMGPATEGFHHEFGPHVIRAYDPCLSCATHVIVVDDDDRSVIKNEMVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T830</accession>
    <name>F0T830_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1417</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645007.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1417"/>
    <dbReference type="EnsemblBacteria" id="ADZ09656">
        <property type="protein sequence ID" value="ADZ09656"/>
        <property type="gene ID" value="Metbo_1417"/>
    </dbReference>
    <dbReference type="GeneID" id="10277868"/>
    <dbReference type="KEGG" id="mel:Metbo_1417"/>
    <dbReference type="eggNOG" id="arCOG02349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13581">
        <property type="entry name" value="HATPase_c_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="118"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="191"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="244"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="483"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="573" mass="65576" checksum="7490EFE8D9868584" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T831</accession>
    <name>F0T831_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645008.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09657">
        <property type="protein sequence ID" value="ADZ09657"/>
        <property type="gene ID" value="Metbo_1418"/>
    </dbReference>
    <dbReference type="GeneID" id="10277869"/>
    <dbReference type="KEGG" id="mel:Metbo_1418"/>
    <dbReference type="HOGENOM" id="CLU_2140213_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12866" checksum="679972289F06066D" modified="2011-05-03" version="1" precursor="true">MKIKILAGLLVSIFILGGISSACAVNYDGYSTINVAKENTFELDLLYNEWIDDDGYNHDLLNLKSKERSEPIDGYWVNMYTFKPLKTGTTKIIIKQQVLWWEEERTVDVNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T836</accession>
    <name>F0T836_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1424"/>
    <dbReference type="EnsemblBacteria" id="ADZ09662">
        <property type="protein sequence ID" value="ADZ09662"/>
        <property type="gene ID" value="Metbo_1424"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1424"/>
    <dbReference type="eggNOG" id="arCOG00027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019582_2_2_2"/>
    <dbReference type="OMA" id="TMAPHSE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="PTHR43321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="481" mass="54813" checksum="AA97A3BEB484305D" modified="2011-05-03" version="1">MIIFVKIKIVEENKKMLSEKKNLDEMDDHEKEQTTTYGSRYFTKSIPKYIVPDEGMPPRAAYQLIHDELNLDGNPALNLASFVTTWMEPEADLLMVESMGKNYVDNDEYPQTEVIQDRVVNMLARLFNAPEDCKSVGSATIGSSEAIMLGLLAHKWTWRSRMEAEGKPTDKPNIVMGADVHTVWEKFARYFDVELKLIPLREDIYTITAEDVVKEIDENTIAVGAVIGTTFTGQMDPIEDINNALLEVKKTKGWDIPIHVDGASGGFIAPFIYPDLPWDFRLEQVRSINVSGHKYGLVYPGVGWLVFKDKSDLPDDLIFNINYLGGLMPNYSLNFSKGSNTIIAQYYNFIRLGMKGYTKIMENMLENARFLGKKLEETGKFEILNDKILFPLIAVKLKNCDFDAFHLSEHLREKGWIVPAYTLPPNADDITVLRMVIKESFSKDMVEMLFKDIMASIKTLNQSLNERRKAIDEKGNPTLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T858</accession>
    <name>F0T858_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1446"/>
    <dbReference type="EnsemblBacteria" id="ADZ09684">
        <property type="protein sequence ID" value="ADZ09684"/>
        <property type="gene ID" value="Metbo_1446"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1446"/>
    <dbReference type="eggNOG" id="arCOG03181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="19756" checksum="273228339D205568" modified="2011-05-03" version="1">MNCYGIGLIRLKEFFGGSVNLDEKNRVFEEIMGDTIVYVTLFKRELNQLNEQKFMKTLLWLTLIDEYGSPSISNLGRKINVSKSQMTSRVDQLVEDGLIERINDTEDRRIIRIQLTKNGKNFLKDSKKNIEAEMNELIAPLSVEDIEDLKTSVLTIKKIVLKIQQSKKKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T860</accession>
    <name>F0T860_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1449"/>
    <dbReference type="EnsemblBacteria" id="ADZ09686">
        <property type="protein sequence ID" value="ADZ09686"/>
        <property type="gene ID" value="Metbo_1449"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1449"/>
    <dbReference type="eggNOG" id="arCOG02935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_0_2"/>
    <dbReference type="OMA" id="TPWHPHR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PTHR13903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="18"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="171"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="282" mass="31385" checksum="6C52C77CB8E0D3AA" modified="2011-05-03" version="1">MEIVEPVYVLEGAGVLLRRSIATATLDYVDPFLLFDHFGSENPGDYVLGFPMHPHRGIETVTYILNGHVDHKDSMGNAGTIGRGDIQWMTSGSGILHEEMPKEPEEGILEGFQLWVNLPKRLKMTEPRYRGVESSEIPTIEHDDGAVIKVIAGNYEGVEGAVTDIYADPTYLDIHIPANSFLEIPVKEDQSVLAYVFEGEGIFGNFETNKTQDGTGIEATKMLIFGEGNIIRVYAENKPVRFLLIAGKPLNEPVARYGPFVMNTEEEIEQAIRDLQNNTFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T876</accession>
    <name>F0T876_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643839.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0236"/>
    <dbReference type="EnsemblBacteria" id="ADZ08488">
        <property type="protein sequence ID" value="ADZ08488"/>
        <property type="gene ID" value="Metbo_0236"/>
    </dbReference>
    <dbReference type="GeneID" id="10276666"/>
    <dbReference type="KEGG" id="mel:Metbo_0236"/>
    <dbReference type="eggNOG" id="arCOG00328">
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="591"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF03104"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="598" mass="68779" checksum="DE964A4D7AF067FE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A3</accession>
    <name>F0T8A3_METLA</name>
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        <submittedName>
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    </protein>
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        <name evidence="6" type="ordered locus">Metbo_0263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
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    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="27"/>
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            <end position="105"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="121" mass="12677" checksum="80FF624708BEF5AC" modified="2011-05-03" version="1">MIMDTQLASLLTAGALIVIGIFGALFLDNLIKKVISLAFIGDGANLFLIAMGYKAGGIVYIFLPGMSMSEFSQNAAYPLPFALVLTSIVIGASTMAVMLGLIIVLNKRYGSLSASKILGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A4</accession>
    <name>F0T8A4_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0264</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08516">
        <property type="protein sequence ID" value="ADZ08516"/>
        <property type="gene ID" value="Metbo_0264"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0264"/>
    <dbReference type="HOGENOM" id="CLU_3353899_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="36" mass="4124" checksum="0C9FDB3A37F8037C" modified="2011-05-03" version="1">MLSEFSLDSIKVNTQLKIPSQFGVYRVNPRLEEGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B0</accession>
    <name>F0T8B0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0270"/>
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        <property type="protein sequence ID" value="ADZ08522"/>
        <property type="gene ID" value="Metbo_0270"/>
    </dbReference>
    <dbReference type="GeneID" id="10276702"/>
    <dbReference type="KEGG" id="mel:Metbo_0270"/>
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    <dbReference type="OrthoDB" id="11187at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
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            <begin position="33"/>
            <end position="53"/>
        </location>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="54"/>
            <end position="83"/>
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            <begin position="84"/>
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            <end position="171"/>
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            <end position="191"/>
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            <begin position="419"/>
            <end position="448"/>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="453" mass="49140" checksum="48BA8EA5432367EF" modified="2011-05-03" version="1">MFLSTKKCEGIGKCVEECPTEAIRIIDGKAFSCITCGACKDACPNSAIFKNKFGGFVVDRAKCNACGVCEMTCPVNNIKIEDGVVKGICARCGICVDACPVKARADAQDVIEDRQLKFLESLNLTIQPGSRVKKEEEYATRTNICTDPENCTLCGRCEYYCPTNAIIVDVDSEGLCTECRICEDVCPVGAIKDGVIDDTKCTLCLKCVSECPNSAMYTEDFKLHIRKPEEGETIEGSIVSCLNCGLCAEACTHGALKVVDGKLRYDPTLCKECSTMDCLEVCPVGTIRESADPDRAVEGFCVSCGKCVQVCDVNKARKLKNIKWDGTVSEDCITCGICSELCPKGAITLRRGSIDVDMDKCILCEKCAIHCPVSAIPRTATLKKSIKEGFTFVQDKMCMKCKLCTKICPEDAINENSEGNIVVDDSKCIYCGACSNACPAKAIILEREFEVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H5</accession>
    <name>F0T8H5_METLA</name>
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            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1494"/>
    <dbReference type="EnsemblBacteria" id="ADZ09726">
        <property type="protein sequence ID" value="ADZ09726"/>
        <property type="gene ID" value="Metbo_1494"/>
    </dbReference>
    <dbReference type="GeneID" id="10277945"/>
    <dbReference type="KEGG" id="mel:Metbo_1494"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="140"/>
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    </feature>
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            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="17117" checksum="D99DE32349E501D4" modified="2011-05-03" version="1">MEDEKSEKLIEEMLEDSIIYIKIFNRELTNLSDKKFMKTFFWLVTIKNYDNLSMSGLAKTLNISKTQITPRIDELVNNGLLERVPDENDRRVLRIALTAEGEDLIHNSNKTVKEALNLLLNPLSPDELEELGKSIETIKNTVLKIQGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I3</accession>
    <name>F0T8I3_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">AL-21</strain>
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    <reference evidence="3 4" key="2">
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09734">
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        <property type="gene ID" value="Metbo_1502"/>
    </dbReference>
    <dbReference type="GeneID" id="10277953"/>
    <dbReference type="KEGG" id="mel:Metbo_1502"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="14145" checksum="0C84FDED0BC97D11" modified="2011-05-03" version="1" precursor="true">MKAKGYIIILLVIVWVGIIALASAYMFPSTPKNNTVTLNDTNVSNNTTINTTSMIQNQSKKPVITETQAINTFKTSAGKYYGNNSRYVATLYHVQGKPYYSITIISKSNGTSENSQSAVNAVTGKLESPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I5</accession>
    <name>F0T8I5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645087.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1504"/>
    <dbReference type="EnsemblBacteria" id="ADZ09736">
        <property type="protein sequence ID" value="ADZ09736"/>
        <property type="gene ID" value="Metbo_1504"/>
    </dbReference>
    <dbReference type="GeneID" id="10277955"/>
    <dbReference type="KEGG" id="mel:Metbo_1504"/>
    <dbReference type="eggNOG" id="arCOG02486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801706_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="16063" checksum="CD690CD8BDC0F9F9" modified="2011-05-03" version="1" precursor="true">MELKRIVPILAIMAMMGVFLVAMQPASAASKSEIKVYQYDPPGAEVVGKEISIYKGEYLDLAATLHIDDGNPQWFRWVNFYVYNSKGDQIVNEERNSGFGGIARCWINSKDWSKGTYNVSVVYQGNEDDGYSKAEKKVVLHVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8N5</accession>
    <name>F0T8N5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">taw2</name>
        <name evidence="5" type="ordered locus">Metbo_0325</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643928.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0325"/>
    <dbReference type="EnsemblBacteria" id="ADZ08577">
        <property type="protein sequence ID" value="ADZ08577"/>
        <property type="gene ID" value="Metbo_0325"/>
    </dbReference>
    <dbReference type="GeneID" id="10276762"/>
    <dbReference type="KEGG" id="mel:Metbo_0325"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_2_2"/>
    <dbReference type="OMA" id="IHYHETV"/>
    <dbReference type="OrthoDB" id="55502at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
        <property type="entry name" value="TYW2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030867">
        <property type="entry name" value="TYW2_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="244" mass="26996" checksum="857546962D6BFDA8" modified="2011-05-03" version="1">MKGKVIGDILVVKDDVEDVENLLKTPGVNRIVKLGRISGLKREPDVEVIIGEGTETVHRENKCGFKLDVSKIMWSKGNTTERKRMSLLPEDGEIIVDMFAGIGYFSIPMAVHSNPKQIYSLEINPVSHGYLCENIKINKVDGVVKPILGNCREVAPKAVADRVLMGYIGNTHEYLDVAMDVLKKGGTLHYHESVPDNIKFKRPVERIIAAAKTAGRGDVEILNARIIKPYSPGVYHVVVDAKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T3</accession>
    <name>F0T8T3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydroorotase</fullName>
            <shortName evidence="3">DHOase</shortName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pyrC</name>
        <name evidence="5" type="ordered locus">Metbo_0373</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643976.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0373"/>
    <dbReference type="EnsemblBacteria" id="ADZ08625">
        <property type="protein sequence ID" value="ADZ08625"/>
        <property type="gene ID" value="Metbo_0373"/>
    </dbReference>
    <dbReference type="GeneID" id="10276810"/>
    <dbReference type="KEGG" id="mel:Metbo_0373"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="43469at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="4">
        <location>
            <begin position="47"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="318"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="3">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="47770" checksum="81A9018306600141" modified="2011-05-03" version="1">MLDMCIVNCRLNPSDQECSIGIENGKIVSIQRLPSVADEIVDLNGKIILPGLIDVHVHMRDPGLTYKENFKTGTESAAAGGFTTVLDMPNTKPPTNTAHDFKNKLKIAGSKSIVDFGLHAGTANPEEIPGLGDLNPASFKVFMDLHDDEFLMNLFSEISDLNQSGTHDHLVSIHAEDQEIVKKYTTMKSSETRSDSMVYADARPPLAEDVAVSKAIMMAKEFNLKVHICHASTETSLNSIQNAKTEGCNISSEVTPHHLFLDSSYLKKFANFAKTNPPLRSDSKKIRLKDLPITDIVGTDHAPHTIAEKEKNVWNAPPGIPGLETALPLLLNQINLGNISFNEIRRLLCENPAERFKIKNKGHIKTGMDADFVVVDMKKEWIIDSDNFYSMAHYSPFDGMKVTGVPIMTILRGEIIMKDSEVFKAIGNHVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T4</accession>
    <name>F0T8T4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09762">
        <property type="protein sequence ID" value="ADZ09762"/>
        <property type="gene ID" value="Metbo_1532"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG12082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392927_0_0_2"/>
    <dbReference type="OrthoDB" id="130127at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="10409" checksum="F338A2B064C23AC9" modified="2011-05-03" version="1">MVDIVIMVSIISSISSIVLAIFAILFSMRVENRLKNNFLKLKDVMDNNHERTKEVLANMDREADEIKASVYESQEELKKELIKIIDDHDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T7</accession>
    <name>F0T8T7_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09765">
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        <property type="gene ID" value="Metbo_1535"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_216165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5641" checksum="57CD127058E9A0CA" modified="2011-05-03" version="1">MDDDELKKKNELERKMERKIDCLIMLGVVILLLVVIVIGILIRTSPFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U0</accession>
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    <reference evidence="3" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1538"/>
    <dbReference type="EnsemblBacteria" id="ADZ09768">
        <property type="protein sequence ID" value="ADZ09768"/>
        <property type="gene ID" value="Metbo_1538"/>
    </dbReference>
    <dbReference type="GeneID" id="10277993"/>
    <dbReference type="KEGG" id="mel:Metbo_1538"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="OMA" id="AYLMVEY"/>
    <dbReference type="OrthoDB" id="106121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ArsR-like_HTH"/>
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        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="17"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="11237" checksum="44CE77B30656A42A" modified="2011-05-03" version="1">MKKVFLWWLITGNKGGENRGRIILELHNRPYNLNKLAEKLCLDYKTIKHHIDVLNENNLVKSTGEKYGALYFLSDEMEKNYAVFLEIWEEFKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W2</accession>
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        <submittedName>
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    </protein>
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        <name evidence="3" type="ordered locus">Metbo_1563</name>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1563"/>
    <dbReference type="EnsemblBacteria" id="ADZ09790">
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        <property type="gene ID" value="Metbo_1563"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1563"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OMA" id="KYLIMAR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
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        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="589" mass="68796" checksum="370C57CBD4CD7622" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X1</accession>
    <name>F0T8X1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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    <accession>F0T900</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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    <sequence length="572" mass="63709" checksum="0781404BCCA0E6B3" modified="2011-05-03" version="1" precursor="true"> </sequence>
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
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                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="391" mass="43593" checksum="5241D29F0F500FB8" modified="2011-05-03" version="1">MANKDLNGNSGSYWLKTCPATNFQPLKLGLKVDTVIIGGGIAGITAGILLKDMGYDVAVLESDRIVKEVTVGTTAKISVAPNMIYHDLMEKFGKEIALKIAKANKNALKKIEELVSTKGIDCEFHKTPLYIYTESSAGIKRLEEEYIAAKDLGFDVELNHHNPLPFKTSCSLKYPNQAQFHPRKYLLGLAEEIEGNGSHVFEKTRAISVHDGDVKEVVTDQGSIMASNVIIATHTPFYDPDHLKDHLNPGRSYVIGLYTRKEFPDGMFVEFNPVHTYRTTPTPKGQLVIVAGEHSEMNVESRQIYYDKLESYAKNHLDVESVRYRWSSHDSVTDDGLPMIGMTSLNAVYVATGFGFWGMNNGTTAGMVIADLIHDNESYLKDIFDPLRFKY</sequence>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1 2" key="2">
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            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="EMBL" id="ADZ08669.1"/>
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        <source>
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    <sequence length="57" mass="6798" checksum="2299E3B714EB5798" modified="2011-05-03" version="1">MDQTDYKIISNSNRCQKCYFRFEVVLDDIEEINISCRHPMGPGNSMHCIYFKNMDMD</sequence>
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<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T978</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50113"/>
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        <source>
            <dbReference type="EMBL" id="ADZ09851.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
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    <sequence length="1116" mass="127185" checksum="6D344C7AF298DE13" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G9</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="27624" checksum="F99FBCDD9421C4A7" modified="2011-05-03" version="1">MIIAVSGKGGTGKTLLSSLLIKELSEKGKDVLAIDADPDSNLPEALGVEVLKTVGDVREELKIDTAQGNIPKEMNKWDILDYKIMEAVIETPKFDLLVMGRPEGSGCYCAVNNILRKIIETLSKNYDYIVIDTEAGLEHLSRRTTQNVDTMLVVTDKSKRGMLTAQRIGELANELDINFKNIFLVVNRITPKNEDLILKKAEDTGIEIIGTIYEDSQVAEYDVEGIPLINLSNDSESVQTVSNIVSRILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
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    <name>F0T9J5_METLA</name>
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        <submittedName>
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    <organism evidence="2 3">
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Metbo_0491"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0491"/>
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        <property type="component" value="Chromosome"/>
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    </dbReference>
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        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="160"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="535" mass="59922" checksum="A303606A7F027533" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L9</accession>
    <name>F0T9L9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase U32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0515</name>
    </gene>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644118.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0515"/>
    <dbReference type="EnsemblBacteria" id="ADZ08767">
        <property type="protein sequence ID" value="ADZ08767"/>
        <property type="gene ID" value="Metbo_0515"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0515"/>
    <dbReference type="eggNOG" id="arCOG03203">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011540_4_0_2"/>
    <dbReference type="OMA" id="MFHMEHC"/>
    <dbReference type="OrthoDB" id="8700at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020988">
        <property type="entry name" value="Pept_U32_collagenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12392">
        <property type="entry name" value="DUF3656"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3656" evidence="1">
        <location>
            <begin position="414"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="884" mass="98018" checksum="1E805B891F16CC51" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q7</accession>
    <name>F0T9Q7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645287.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1712"/>
    <dbReference type="EnsemblBacteria" id="ADZ09936">
        <property type="protein sequence ID" value="ADZ09936"/>
        <property type="gene ID" value="Metbo_1712"/>
    </dbReference>
    <dbReference type="GeneID" id="10278169"/>
    <dbReference type="KEGG" id="mel:Metbo_1712"/>
    <dbReference type="eggNOG" id="arCOG02646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_3_2"/>
    <dbReference type="OMA" id="FQMSELI"/>
    <dbReference type="OrthoDB" id="93551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="22310" checksum="DC8C70766AA4B032" modified="2011-05-03" version="1">MVSTEDKILDATIELLDNEGLNGATTRKIASEAGVNEVTVFRKFKNKSRLLRAAKERGANQFLLELETMFDIDEDDDVEKYLMTVWKNASKKIDKRINLIRISMEEVRGIPIEDKVFPKISHLIKQHLTKYFQKLIEKGIVKDIDPEAAAMTIFSIVFHITIYGKIYEQYPEESMEKNMENSLKIFLHGVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X1</accession>
    <name>F0T9X1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644145.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0542"/>
    <dbReference type="EnsemblBacteria" id="ADZ08794">
        <property type="protein sequence ID" value="ADZ08794"/>
        <property type="gene ID" value="Metbo_0542"/>
    </dbReference>
    <dbReference type="GeneID" id="10276987"/>
    <dbReference type="KEGG" id="mel:Metbo_0542"/>
    <dbReference type="eggNOG" id="arCOG00037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381418_0_0_2"/>
    <dbReference type="OMA" id="FYDVTLV"/>
    <dbReference type="OrthoDB" id="71195at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="22454" checksum="F01F6EB3B7745DBB" modified="2011-05-03" version="1">MKACVLYSGGKDSSLMAFILNKLGYDVELVTVNYGIYPSWKPAAESASNLNFNHRVMNADSDTMRSAVQTILSDGFPNNGINQLHKYALELVAKEYDVVADGTRRDDRIPKLNMNEVRSFEDRNNVEYINLGGFGHKSMNHLSKTLFEVKKELTNMDNNSDYEIEIRTLISEIEGDSAALKIFPEHLQSRVIGWRKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA11</accession>
    <name>F0TA11_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644185.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0583"/>
    <dbReference type="EnsemblBacteria" id="ADZ08834">
        <property type="protein sequence ID" value="ADZ08834"/>
        <property type="gene ID" value="Metbo_0583"/>
    </dbReference>
    <dbReference type="GeneID" id="10277028"/>
    <dbReference type="KEGG" id="mel:Metbo_0583"/>
    <dbReference type="eggNOG" id="arCOG03229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102155_0_0_2"/>
    <dbReference type="OMA" id="FVANHYK"/>
    <dbReference type="OrthoDB" id="73889at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF434" evidence="1">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5616" evidence="2">
        <location>
            <begin position="65"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="25284" checksum="A561FB01CCC77620" modified="2011-05-03" version="1">MKNKLLNNAAYDLKFLLNRGYRKKGALNFVSNKYLLNKDERNYLFRRTFSDSKSISRKGKIISLDEIVGRNIFVDGYNVLITSEIIINQEYDSLVMCDDGVLRDLKAVFGKYNMDINTEKVMLAVTNLMNQHHPLFINFLFDSPVSKSGELAKLTNSILEDHRVNGTAETVRNVDYELVKRSNASCGVVATSDGVIMDRVENILDIPYWFCMENNCSKNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA16</accession>
    <name>F0TA16_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">radB</name>
        <name evidence="6" type="ordered locus">Metbo_0588</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644190.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0588"/>
    <dbReference type="EnsemblBacteria" id="ADZ08839">
        <property type="protein sequence ID" value="ADZ08839"/>
        <property type="gene ID" value="Metbo_0588"/>
    </dbReference>
    <dbReference type="GeneID" id="10277033"/>
    <dbReference type="KEGG" id="mel:Metbo_0588"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_2_0_2"/>
    <dbReference type="OMA" id="GNTLEHW"/>
    <dbReference type="OrthoDB" id="93716at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RECA_2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="232" mass="25816" checksum="DD01B4E983422F74" modified="2011-05-03" version="1">MKILSELDETKKIPTDSPLDNLMGGGIEKGCLTQFYGPPGSGKTNIALQLLVRCAQNGDKAIFIDTEGGLSIERVKQISNNEFTSFAENIIIFEPTTFKEQIDVIKKIENLLESKKECVELIILDSAVALYRLKEGDSTQINRELGQQMALLSRIARKYDVAVLITNHVYSVFDGDGIIEPVGGTILKYWSKVVVELARSNGLGERSATLKRHRSRPEGLRTRFRIVDCGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA17</accession>
    <name>F0TA17_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644191.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08840">
        <property type="protein sequence ID" value="ADZ08840"/>
        <property type="gene ID" value="Metbo_0589"/>
    </dbReference>
    <dbReference type="GeneID" id="10277034"/>
    <dbReference type="KEGG" id="mel:Metbo_0589"/>
    <dbReference type="HOGENOM" id="CLU_2044430_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="120" mass="12633" checksum="ED44ADD0AC49C36B" modified="2011-05-03" version="1">MLTKMPSETKSKTEPSQLNTKFFVGVVAVFAVLGIAGGYMMFPLLQTPNVATVAVNTSNTTNNTTNYTNPVVKHSTIVSNNSGESNQNNNGSTTSKTTNKSTSKQTHSNVGSSQTGKNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA32</accession>
    <name>F0TA32_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Potassium-transporting ATPase KdpC subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP phosphohydrolase [potassium-transporting] C chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-binding and translocating subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-translocating ATPase C chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kdpC</name>
        <name evidence="12" type="ordered locus">Metbo_1759</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the KdpC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1759"/>
    <dbReference type="EnsemblBacteria" id="ADZ09982">
        <property type="protein sequence ID" value="ADZ09982"/>
        <property type="gene ID" value="Metbo_1759"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1759"/>
    <dbReference type="eggNOG" id="arCOG04805">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077094_2_0_2"/>
    <dbReference type="OMA" id="KYFWPRP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00276">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003820">
        <property type="entry name" value="KdpC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30042">
        <property type="entry name" value="PTHR30042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02669">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001296">
        <property type="entry name" value="K_ATPase_KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00681">
        <property type="entry name" value="kdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="20543" checksum="B78D82195D5942F8" modified="2011-05-03" version="1">MGEIMGEIKRAVLLFAVLAVVLGLIYPLVITGISQVVFPFQANGEMIKINGTVVGSELIGQDFEGSQYFQGRPSAVEYNASTSGGSNYGPTNQKLIDRVNQSLTSIRANNSLSSNATIPSDMVLASGSGLDRYISVQSAIIQVPRIAKQRNISETVVMELINNTEETQFPWIGQPVVNVLKLNIGLDRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA81</accession>
    <name>F0TA81_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">APHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645382.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1809"/>
    <dbReference type="EnsemblBacteria" id="ADZ10031">
        <property type="protein sequence ID" value="ADZ10031"/>
        <property type="gene ID" value="Metbo_1809"/>
    </dbReference>
    <dbReference type="GeneID" id="25394752"/>
    <dbReference type="KEGG" id="mel:Metbo_1809"/>
    <dbReference type="eggNOG" id="arCOG02532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_710994_0_0_2"/>
    <dbReference type="OrthoDB" id="100286at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CARDB" evidence="5">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="42228" checksum="F731AFAA77D5545E" modified="2011-05-03" version="1" precursor="true">MKKTVIMALILMVTPFIVAGEVSATEINSYSNNKTLKADLKVTNVVVHGSLVRGCNIAVNNTITNSGNGSAGGFWVNYYLKTNPTSNSYLIGKRYINGLKTGSSNTQNTLLYIPTNMPLGSYLLMASADASNSIAESNKSNNKGYSSLLKVVGPTYIIFDKNVGGDVLKNPEINKYIPKTSFAKTIFNLEKSGSVMLKFGNGNGPKVLISAGIHGNETEANIAIMKYLEYIKDKSIKGTLYVIPFDIPLDTAKNTRFYHGHDPNRIANVAGSPGWNIIQFAHKNSINYLLDVHSGGEVTSKGLLNVNTGTSNSQEVKWAKYITSTSGCVSRVQPLETGMVRAEAFKYNISTITMEVERDTAPTIVSALTEFKMIKAAMKFFKFPVPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA3</accession>
    <name>F0TAA3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN_9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644206.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0604"/>
    <dbReference type="EnsemblBacteria" id="ADZ08855">
        <property type="protein sequence ID" value="ADZ08855"/>
        <property type="gene ID" value="Metbo_0604"/>
    </dbReference>
    <dbReference type="GeneID" id="10277049"/>
    <dbReference type="KEGG" id="mel:Metbo_0604"/>
    <dbReference type="eggNOG" id="arCOG04312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_1_0_2"/>
    <dbReference type="OMA" id="DEMIINY"/>
    <dbReference type="OrthoDB" id="118267at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN_9" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13968" checksum="4A15A289911ADE41" modified="2011-05-03" version="1">MMAFQFNVDVVGELRNIIPSCRLIVPSTVLYELEGIKKRSKGKNKIAASIALKIAKTPPIEVKEMKLQTGETVDDSLLRVSMRSKILCTNDRELRNRAREIGINVIYLRQRKYLDVDGHIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB6</accession>
    <name>F0TAB6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho 3-hexuloisomerase</fullName>
            <ecNumber evidence="3">5.3.1.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644219.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0617"/>
    <dbReference type="EnsemblBacteria" id="ADZ08868">
        <property type="protein sequence ID" value="ADZ08868"/>
        <property type="gene ID" value="Metbo_0617"/>
    </dbReference>
    <dbReference type="GeneID" id="10277062"/>
    <dbReference type="KEGG" id="mel:Metbo_0617"/>
    <dbReference type="eggNOG" id="arCOG00068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094236_1_0_2"/>
    <dbReference type="OMA" id="HIFLQGA"/>
    <dbReference type="OrthoDB" id="106225at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043800">
        <property type="term" value="F:hexulose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="PTHR43443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="30"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="194" mass="20972" checksum="BD6DD1FA93E7E219" modified="2011-05-03" version="1">MEHIKKTVENITKHALTVIDKIDGAQVDQMITTIQNAKSVFIVGSGRSELIGKAFAMRLMHLGFVVYVIGDVTTPAIDNEDCLIAISGSGETRTVTLAAGTAANSGATVIGITATPSSTLGIYLDVCVKIESKTKTPWKHYTSAVLKGDYDDLTPMGTLFEDTTHLFLDGLIAEFMARLGKKEDDLKRRHATIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB9</accession>
    <name>F0TAB9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PilT protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0620"/>
    <dbReference type="EnsemblBacteria" id="ADZ08871">
        <property type="protein sequence ID" value="ADZ08871"/>
        <property type="gene ID" value="Metbo_0620"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0620"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="OMA" id="FADMRMA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="611" mass="68160" checksum="19AA6DA953298E98" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG5</accession>
    <name>F0TAG5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="13">IOR</shortName>
            <ecNumber evidence="3 13">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Metbo_0666</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] = (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13 14">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 13">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3 13"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644268.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0666"/>
    <dbReference type="EnsemblBacteria" id="ADZ08917">
        <property type="protein sequence ID" value="ADZ08917"/>
        <property type="gene ID" value="Metbo_0666"/>
    </dbReference>
    <dbReference type="GeneID" id="10277111"/>
    <dbReference type="KEGG" id="mel:Metbo_0666"/>
    <dbReference type="eggNOG" id="arCOG01609">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017727_0_0_2"/>
    <dbReference type="OMA" id="GCKLCLK"/>
    <dbReference type="OrthoDB" id="20764at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="PTHR43710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006439">
        <property type="entry name" value="Indolepyruvate_ferr_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03336">
        <property type="entry name" value="IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 13 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="15">
        <location>
            <begin position="588"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ08917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="616" mass="67508" checksum="4D9EB25650713127" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI7</accession>
    <name>F0TAI7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1837</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645410.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10059">
        <property type="protein sequence ID" value="ADZ10059"/>
        <property type="gene ID" value="Metbo_1837"/>
    </dbReference>
    <dbReference type="GeneID" id="10278294"/>
    <dbReference type="KEGG" id="mel:Metbo_1837"/>
    <dbReference type="eggNOG" id="arCOG02532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833168_0_0_2"/>
    <dbReference type="OrthoDB" id="93254at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="46"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="333" mass="37100" checksum="8BB41653A821DE24" modified="2011-05-03" version="1" precursor="true">MKKLVFIFVFVVMLMVVNPVSAGTHKQMKLSNNSSCDVVLKDVKTDPVIFRNKTIKISSTLSNLGKSSCKEFYMDYFLKSADTGQNVYIGSIHIGGLGSLESRVVNSSFNLPKTLKNAEYYVRIVLDSTNVLNETNKTNNVVYSQKKLSLVTGRPVYITSDHIKNNTADNSKVDAIMKGLQKKGLYAVNYGLGPNKHYSVLKNIKVPDNALIVNIYGGACAGTIWEMAQPYYKKALGSRKVFSIWINTNVNIKTVKFLKRASDDNDTPQYGKKGGFPQFQDTNHNGKFEPNLGEKDGLSNPGKLLDNNGYKYLYQQDGNVEKIVEAIYNQANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK5</accession>
    <name>F0TAK5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamate--ammonia ligase</fullName>
            <ecNumber evidence="3">6.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645428.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1856"/>
    <dbReference type="EnsemblBacteria" id="ADZ10077">
        <property type="protein sequence ID" value="ADZ10077"/>
        <property type="gene ID" value="Metbo_1856"/>
    </dbReference>
    <dbReference type="GeneID" id="10278314"/>
    <dbReference type="KEGG" id="mel:Metbo_1856"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_6_0_2"/>
    <dbReference type="OMA" id="DWGLDNR"/>
    <dbReference type="OrthoDB" id="47310at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="2">
        <location>
            <begin position="105"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="440" mass="49004" checksum="BDA597C7964CBB6D" modified="2011-05-03" version="1">MGKQLEDLGERSRFLRILWIDNANMIRSKALRLKVDDDVSEAVVGISRAQQGVPVVYDGVVPGAPLDPVGEVYLKADNSTIRKVPYAPGNSLAIGDMYVDGSPWDFCPRNYLRKILNLARDEGLEIKASFENEFYLLNKDDPLNCHENTAFGSTNSMNLNNDVIMEIADYLESQGLSVEQYYSESGPCQHEITVHYDDAMKATDNQIIFRETVRGVALKHGMVASFLPKLFADHAGSGCHIHLSLWRDGVNILHDPDENFELGNTAHHFIAGILEHLSALMAITTPTPNSYRRILPSSWAGKYGCWGFDNREASIRVVKEPDGTIKHFEIKTSDATSNPYLSIGSIIFSGLNGIKEKLSLPEPVQLDPAKLEEDERLKLNIKDLPSCVDDAIKSLSKDEVLMNAMGDGLSKAYIAVKTEENNYLKNLSLEEEVNLLLDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM1</accession>
    <name>F0TAM1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1872"/>
    <dbReference type="EnsemblBacteria" id="ADZ10093">
        <property type="protein sequence ID" value="ADZ10093"/>
        <property type="gene ID" value="Metbo_1872"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1872"/>
    <dbReference type="eggNOG" id="arCOG02329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319268_0_0_2"/>
    <dbReference type="OMA" id="HILMSEM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="135"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="205"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="260"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="334"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="388"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="515"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="592"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="643"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="721"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="910" mass="105329" checksum="E55AEC4D7B1F99CB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP0</accession>
    <name>F0TAP0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645463.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1892"/>
    <dbReference type="EnsemblBacteria" id="ADZ10112">
        <property type="protein sequence ID" value="ADZ10112"/>
        <property type="gene ID" value="Metbo_1892"/>
    </dbReference>
    <dbReference type="GeneID" id="10278350"/>
    <dbReference type="KEGG" id="mel:Metbo_1892"/>
    <dbReference type="eggNOG" id="arCOG10461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811464_0_0_2"/>
    <dbReference type="OrthoDB" id="113318at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="15751" checksum="28D021A0075F5C95" modified="2011-05-03" version="1">MANFSVETAIVISFILGLALPVIHLGGVFSIFIMGFAATYLTKTENTSAMVGGIAAIVFGVFFFFFGFLTGPTLPYTLPSPLALGILVPLTGLFYLLMGLIVSIIIYGVIGMLGGYIALKFFKEKKEKKQEFEPRRPQRTLKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR9</accession>
    <name>F0TAR9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="3">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644296.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0694"/>
    <dbReference type="REBASE" id="33860">
        <property type="entry name" value="M.Msp21ORF694P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08945">
        <property type="protein sequence ID" value="ADZ08945"/>
        <property type="gene ID" value="Metbo_0694"/>
    </dbReference>
    <dbReference type="GeneID" id="10277139"/>
    <dbReference type="KEGG" id="mel:Metbo_0694"/>
    <dbReference type="eggNOG" id="arCOG03416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063430_0_0_2"/>
    <dbReference type="OMA" id="MNRHGFN"/>
    <dbReference type="OrthoDB" id="85238at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1020.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023095">
        <property type="entry name" value="Ade_MeTrfase_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012263">
        <property type="entry name" value="M_m6A_EcoRV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012327">
        <property type="entry name" value="MeTrfase_D12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30481">
        <property type="entry name" value="PTHR30481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02086">
        <property type="entry name" value="MethyltransfD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000398">
        <property type="entry name" value="M_m6A_EcoRV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00505">
        <property type="entry name" value="D12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00571">
        <property type="entry name" value="dam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="302" mass="35577" checksum="B0835EB660157F65" modified="2011-05-03" version="1">MCTKPFLKWAGGKSQLLPEFNQRLPNHIIENKTIETYVEPFVGGGAMFFNLKKNYKLKESVLSDINRELVMAYQVIKNDYEELIDLLKNLEEHHLNLDEDGRKENYYYMRKEYNSRFMDMDFLNYNESWVERTSYLIFMNKTCFNGLYRQNKQGGYNVPFGRYKNPTICDSENIRLVNKALKNTEILCVDFTETGKYVHENTFVYLDPPYRPLNRTSNFTSYSKEGFNDIDQIKLASFYSEMNDLGACLMLSNSDPKNYDAEDEFFDELYKDFKIERVAAKRNINSNASGRGLINELIITNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS2</accession>
    <name>F0TAS2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0697</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644299.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0697"/>
    <dbReference type="EnsemblBacteria" id="ADZ08948">
        <property type="protein sequence ID" value="ADZ08948"/>
        <property type="gene ID" value="Metbo_0697"/>
    </dbReference>
    <dbReference type="GeneID" id="10277142"/>
    <dbReference type="KEGG" id="mel:Metbo_0697"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_2"/>
    <dbReference type="OMA" id="RWEANIS"/>
    <dbReference type="OrthoDB" id="38088at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="45034" checksum="33772C9E8F841B67" modified="2011-05-03" version="1" precursor="true">MKFTSIAVKDFKELIRDKRGLFFILLFPIFFMMIFGFAFGGMGQENTPHNIAIVNYDEGTIIPTGENFNFGNNLTNLIKETSYDNSDVKLFNVTETSETQADSLLKQRTIDAELIIPSNFSASMASQIANSTGLTANINNTVSSPIIRGDEGYSNFFASESALSTVITGYQTGTVTYYKNQIAGTPGAEPVKYVNITVQGISGTQSFTQFDFLAPGMMVFAILLLATTIAAILTREVESGTLERLKMSKMRSFDLLFGGLIPWSLVAGAQVVILLIVAVLLGLHWQGGINSVILATIIGIIGGVASIALAMIIASFAKNDRQAANLGTLIVVPISFLVGAFFPLPQVFISVLGHSFQVYELIPWTHTLNALRGVLLYGEGFNAVAVQIELSLVLTVILFIIGVFLYSRTRLRADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY1</accession>
    <name>F0TAY1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644358.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0756"/>
    <dbReference type="EnsemblBacteria" id="ADZ09007">
        <property type="protein sequence ID" value="ADZ09007"/>
        <property type="gene ID" value="Metbo_0756"/>
    </dbReference>
    <dbReference type="GeneID" id="10277204"/>
    <dbReference type="KEGG" id="mel:Metbo_0756"/>
    <dbReference type="eggNOG" id="arCOG12008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912356_0_0_2"/>
    <dbReference type="OMA" id="FERNTQH"/>
    <dbReference type="OrthoDB" id="110914at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14933" checksum="45D783DCFCD5BBA0" modified="2011-05-03" version="1">MDISSLIPDFLVFSLAGSSLSITAYSAFRDRNDDKKLLLIIATCFSLAFVVVFVMTFINMDNLTEIPVLGLTFYILIFMAILSIIGFIIHRLMVDERYKQDELESAIDIIESSKNGLNSFIGLLKCEYKDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB18</accession>
    <name>F0TB18_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methanogen homoaconitase small subunit</fullName>
            <shortName evidence="2">HACN</shortName>
            <ecNumber evidence="2">4.2.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hacB</name>
        <name evidence="4" type="ordered locus">Metbo_1944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate. All these reactions are part of the biosynthesis pathway of coenzyme B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R)-homocitrate = (2R,3S)-homoisocitrate</text>
            <dbReference type="Rhea" id="RHEA:32303"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of 2 HacA and 2 HacB proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.114" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645515.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1944"/>
    <dbReference type="EnsemblBacteria" id="ADZ10164">
        <property type="protein sequence ID" value="ADZ10164"/>
        <property type="gene ID" value="Metbo_1944"/>
    </dbReference>
    <dbReference type="GeneID" id="10278404"/>
    <dbReference type="KEGG" id="mel:Metbo_1944"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="DDVNTDY"/>
    <dbReference type="OrthoDB" id="86621at2157"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="3">
        <location>
            <begin position="48"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="YLRT" evidence="2">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="site" description="Critical for substrate specificity" evidence="2">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="17996" checksum="5BBBF4FD5853E333" modified="2011-05-03" version="1">MEKLEGKVWKFRDSIDTDVIIAGRYLRTFSLDDLASHVMEAEDPNFAENVDEGDIIVAGWNFGCGSSREQAPVALKHAGVSAIIAKSFARIFYRNAINIGLPVITADVTAEKGDEICIDLEKGVILNKSTGSEFKIQPFDNFMLEILSDGGLVKHYLKVKESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB22</accession>
    <name>F0TB22_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H4MPT-linked C1 transfer pathway protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645519.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1948"/>
    <dbReference type="EnsemblBacteria" id="ADZ10168">
        <property type="protein sequence ID" value="ADZ10168"/>
        <property type="gene ID" value="Metbo_1948"/>
    </dbReference>
    <dbReference type="GeneID" id="10278408"/>
    <dbReference type="KEGG" id="mel:Metbo_1948"/>
    <dbReference type="eggNOG" id="arCOG04369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060932_0_0_2"/>
    <dbReference type="OMA" id="TDCFADR"/>
    <dbReference type="OrthoDB" id="59450at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002756">
        <property type="entry name" value="MfnF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03123">
        <property type="entry name" value="one_C_unchar_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="64"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="37724" checksum="DF83EF0264FCE61D" modified="2011-05-03" version="1">MRIAGFDIGGANTDLAVVELDESGDITDVKTDFSYLPMWERKDDLADVLKELLEPFGEVDAVGISMTAELVDAYKTKKEGVVDIASKSNDAFDVPVGFVGLDGMMNLKEVLDDPLKAAAANWIATAPLAAKIKPDTILLDTGSTTTDIIPIKNGVECALGRSDLERLQTGELVYSGTLRTNVAALVEKVPLNDTMARVASELFAVTADVHRVLGNITKENYSGVTMDGAGKSIVDCRRRISRVVCGDLEVLSTDEIENIAAYIYSEQVRKISEALKEVSIRNRLHHVVTTGLGMDIIASMACELAGLTFTGMDKLLKKEDCVNAPAVGTALMMEPYLTKKNLEENLEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB61</accession>
    <name>F0TB61_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="5">RNA cyclase</shortName>
            <shortName evidence="5">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="5">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rtcA</name>
        <name evidence="8" type="ordered locus">Metbo_0761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0761"/>
    <dbReference type="EnsemblBacteria" id="ADZ09012">
        <property type="protein sequence ID" value="ADZ09012"/>
        <property type="gene ID" value="Metbo_0761"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0761"/>
    <dbReference type="eggNOG" id="arCOG04125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="OMA" id="PKPGLSH"/>
    <dbReference type="OrthoDB" id="52581at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="PTHR11096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="PTHR11096:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RTC" evidence="6">
        <location>
            <begin position="8"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="RTC_insert" evidence="7">
        <location>
            <begin position="184"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="5">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="281"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="337" mass="35804" checksum="6BDD4EA3856108D1" modified="2011-05-03" version="1">MEIDGSRGEGGGAVLRIATGLSALTSKPVKIYNIRSKRPKPGLMPQHLNSVKAVAELSDANLSGLKIGSTELSFQPGKLKPGNYDIDIKTAGSISLILQAFMIPAAFTGGPVKITIKGGTDLRWSPSIDYLQNITLPILNTMGYKADTQVLRRGHYPKGGGILEVTIYPISSLKPVNIIESNFNAVKGISHAVNLPEHVAIRQARSAEVMIKNAGYDINVDIEHDDNSFGSGSGILLWTDGTIPLGGSSIGERGKKAEVVGLDAAKDLLEQISGNSALDSHMGDQIIPYLFIAGNSSITTSKLTQHTLTNIDVASQFINRDVQVDGKLGKPALITVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB88</accession>
    <name>F0TB88_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl5</name>
        <name evidence="8" type="ordered locus">Metbo_0788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644390.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0788"/>
    <dbReference type="EnsemblBacteria" id="ADZ09039">
        <property type="protein sequence ID" value="ADZ09039"/>
        <property type="gene ID" value="Metbo_0788"/>
    </dbReference>
    <dbReference type="GeneID" id="10277236"/>
    <dbReference type="KEGG" id="mel:Metbo_0788"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="97230at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_L5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_L5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="6">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="7">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="168" mass="18918" checksum="9617D51919726BB1" modified="2011-05-03" version="1">MNPMDQVKIAKATVNIGVGESGERLARAEKLLINITDQKPIRTISKVTNPEFGIRKGQPIACKVTLRNEKASKAVKMILEGIGNNIKSTQFDAQGNVAFGIHEHIDIPGMRYDPDIGIFGMNVAITFEKPGYRIKRRKIQNKKVPVKHQVTKEETMEFMKNEFQVNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA2</accession>
    <name>F0TBA2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytidylate kinase</fullName>
            <shortName evidence="5">CK</shortName>
            <ecNumber evidence="5">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytidine monophosphate kinase</fullName>
            <shortName evidence="5">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cmk</name>
        <name evidence="6" type="ordered locus">Metbo_0802</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644404.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0802"/>
    <dbReference type="EnsemblBacteria" id="ADZ09053">
        <property type="protein sequence ID" value="ADZ09053"/>
        <property type="gene ID" value="Metbo_0802"/>
    </dbReference>
    <dbReference type="GeneID" id="10277250"/>
    <dbReference type="KEGG" id="mel:Metbo_0802"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OMA" id="FIFRDMA"/>
    <dbReference type="OrthoDB" id="110333at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="176" mass="19754" checksum="B7C39392ABFCCE57" modified="2011-05-03" version="1">MIITIGGLAGSGTTTAAKILSEKLQIPFLSAGEIFRQMAKEKGMDILEFGEYAEGNDEIDLEIDKRQAQLAKEKQNLIVEGRLSAYFVEADLKIWFVAPIDVRCKRISKREDKTVEVVKNEIIARGQSEATRYHEIHGIDINNMEVYDLVINSHSFNPEGIADIILKVTEVITCQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB8</accession>
    <name>F0TBB8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Mevalonate kinase</fullName>
            <shortName evidence="11">MK</shortName>
            <shortName evidence="11">MVK</shortName>
            <ecNumber evidence="2 11">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mvk</name>
        <name evidence="14" type="ordered locus">Metbo_0818</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="2 11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644420.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0818"/>
    <dbReference type="EnsemblBacteria" id="ADZ09069">
        <property type="protein sequence ID" value="ADZ09069"/>
        <property type="gene ID" value="Metbo_0818"/>
    </dbReference>
    <dbReference type="GeneID" id="10277267"/>
    <dbReference type="KEGG" id="mel:Metbo_0818"/>
    <dbReference type="eggNOG" id="arCOG01028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="OMA" id="NTVCTYG"/>
    <dbReference type="OrthoDB" id="90882at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="PTHR43290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="101"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="232"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ09069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="35110" checksum="C6044F5C7637E63C" modified="2011-05-03" version="1">MLDSRQVTASAPGKTILFGEHAVVYGKPAIAAAVDKRAYVTISKREDHLTHVTVGDLGVSGFLDLKNRTIQLENGSGMQRGILEYILKALIKAKTEDSVEVDVRLDIPIGAGLGSSAAVTVATIMAAASFNQITLTNETMANLAHQVELDVQGAASPIDTSLSTYGGVIYLSKQPQKLVKLEIPHELPIVIGYTSTRGNTGELVKLVRQKKDARPEVINPILDSMEAVANGARQAIIKGDHKTIGLLMNINQGLLDALGVNTEELSKMVFTARNQGAMGSKLTGAGGGGSMIAYCPGREEEVVSSINQFERAFQINVAHEGVRLEKPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG0</accession>
    <name>F0TBG0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2010"/>
    <dbReference type="EnsemblBacteria" id="ADZ10229">
        <property type="protein sequence ID" value="ADZ10229"/>
        <property type="gene ID" value="Metbo_2010"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2010"/>
    <dbReference type="eggNOG" id="arCOG03597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157711_1_0_2"/>
    <dbReference type="OMA" id="YCATCKA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="11897" checksum="57A80A9DA4DBA0CD" modified="2011-05-03" version="1">MNKLEFSLENYEKCKCIKCPVQICSTCTVEKQQEIDPKIEAKGTDSDSVNVPGMYCVTGRSSCGDLNTKEICQCYRCQFWTEYDLLHGEPMRYFCSKGVSKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI2</accession>
    <name>F0TBI2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645602.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10251">
        <property type="protein sequence ID" value="ADZ10251"/>
        <property type="gene ID" value="Metbo_2032"/>
    </dbReference>
    <dbReference type="GeneID" id="10278493"/>
    <dbReference type="KEGG" id="mel:Metbo_2032"/>
    <dbReference type="eggNOG" id="arCOG00958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_11_4_2"/>
    <dbReference type="OMA" id="CAGVCPR"/>
    <dbReference type="OrthoDB" id="123760at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="55" mass="6187" checksum="94F5A51774194487" modified="2011-05-03" version="1">MIVKEWCMYCGECAGVCPRALIEVRETSLIFNEEGCKDCRICVSVCPINALDKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK1</accession>
    <name>F0TBK1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="2">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645621.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2051"/>
    <dbReference type="EnsemblBacteria" id="ADZ10270">
        <property type="protein sequence ID" value="ADZ10270"/>
        <property type="gene ID" value="Metbo_2051"/>
    </dbReference>
    <dbReference type="GeneID" id="10278513"/>
    <dbReference type="KEGG" id="mel:Metbo_2051"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="OMA" id="MNRIYYE"/>
    <dbReference type="OrthoDB" id="19112at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="46685" checksum="B0B4786CEF4CF708" modified="2011-05-03" version="1">MKLVVVGGGSAGRTASIEASELGADVTLIETDKIGGKCLNQGCMVVCALNDVVKTVQANHKFEKLGIIDSNPKINFRKVSEGIINTTTKIRGVLTHETKKAGVNIVMGTAKIQEGYVTVNETDYPYDKLIIATGSNPFIPDTKGKEYAKTYKEMLNYTEVPEELIIVGSGTIATEIAGIFSGLGSKVHILCRGIFLKEVDEDIKKYIADILLNDVEIHEHVDVEEIHEDGVTTSEGKLNGEVFLAVGMIPNSQAVNELVDTGKRGEIIVNKRMETSHENIYAAGDVVGGIGTTPVARMEGVVAARNSCGIASEVDYSYIPLSITLHHDVSYVDTGKEGVEGHIPGSGGPNAYWDVLDSETGMTKMSVDLETGEIASIKSISPSSRTVLAYMSKFMKDGYTVHDFENFVEAHPSTDAVYKLIRFFASHEKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL1</accession>
    <name>F0TBL1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0838"/>
    <dbReference type="EnsemblBacteria" id="ADZ09088">
        <property type="protein sequence ID" value="ADZ09088"/>
        <property type="gene ID" value="Metbo_0838"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0838"/>
    <dbReference type="eggNOG" id="arCOG00896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07653">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_2_2"/>
    <dbReference type="OMA" id="FSIESEM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="426" mass="46851" checksum="565757240030C70B" modified="2011-05-03" version="1">MNKIKVLVVDDESVVAVGIKKQLKDLGYEVVGTAATGKEAINIALQKDPDIILMDIVLQGEMDGIETAEVIQSHMDTPIIYITAYFDEEVLRRARLTEPYAYMIKPIKTSELNANLQMAVYKYKSEKKKDEISQQITALIPAYNEQVSIGSVVLKTRMYVDRVIVIDDGSSDRTEEIAVLAGAEILKHPVNQGKGKALETGFNAAKGSEILITLDGDGQHSVTDIPKLIKPIINGEADIVNGSRYLDGDDEDTPAYRRVGQNVLDVATNFNARSDITDSQSGFRAFAAYTIPAFKFRESGYGIESEMIIEASNAGFRIKEVPIGVRYDVNGSKKHPLTHGIGVLVKVLQDMEFNRPLYYFTFPGIVMIIIGLLAGLIFFGDYLNHETSSLAPTTLAALLTLAGTFIAFTGIILHSMSRMIERTMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT5</accession>
    <name>F0TBT5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645628.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2058"/>
    <dbReference type="EnsemblBacteria" id="ADZ10277">
        <property type="protein sequence ID" value="ADZ10277"/>
        <property type="gene ID" value="Metbo_2058"/>
    </dbReference>
    <dbReference type="GeneID" id="10278520"/>
    <dbReference type="KEGG" id="mel:Metbo_2058"/>
    <dbReference type="eggNOG" id="arCOG06728">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_4_0_2"/>
    <dbReference type="OMA" id="VLIHFMP"/>
    <dbReference type="OrthoDB" id="91017at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="26293" checksum="A9A50AAC0A8201D1" modified="2011-05-03" version="1">MYDGSLLFFVAAFLSIVVGTVAGFGTSTIFLPIALFFVDFKTALVLVAVSHLSGNIGATTFFRHGLEKKLILLFGVPSILLTVLGAYLVVYVPQNILIIILGICLLIYSVTFLLKPDIKVAANRNNTIFGGGLSGLLQGLAGIGGPIRSAFLISYDLDKYRYIATLAALAVLIDLTRLPIYLTNNLLEPQYYYYIPILVVLGILGSYVGKRIVNIIPQALFKRMVMVAIGLASIILIIGGLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU0</accession>
    <name>F0TBU0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645633.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2063"/>
    <dbReference type="EnsemblBacteria" id="ADZ10282">
        <property type="protein sequence ID" value="ADZ10282"/>
        <property type="gene ID" value="Metbo_2063"/>
    </dbReference>
    <dbReference type="GeneID" id="10278525"/>
    <dbReference type="KEGG" id="mel:Metbo_2063"/>
    <dbReference type="eggNOG" id="arCOG00245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036672_1_0_2"/>
    <dbReference type="OMA" id="TDGLGKW"/>
    <dbReference type="OrthoDB" id="117835at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008299">
        <property type="entry name" value="Prep_DH/arog_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006549">
        <property type="entry name" value="PDH_arog_dh_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="2">
        <location>
            <begin position="355"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="435" mass="48103" checksum="5E37B7270B124368" modified="2011-05-03" version="1">MVKIAVIGGTRGLGRWIAKFLAEKGFDVLITGRNVTDGELVSKKIGTGYTNNNSLAAETSDVVIISVPIHATPNIIKELAPLMKPGSLLMDVTSVKEESSHLMEQYAAEGVEVVPSHPMFGPRIRSLDGQVVVLTPSVDGSWYTKVYKFLEHENTRIIVTTPEIHDRMMSIVQGLTHFAYVSIAATIDRLDIDIKESRKFASPIYNLMLDTIARITAQNPYLVYSIQTSNKYIKDAHETFNETFNELKNMIADGDEEGFVHAMSNAAKHIDDLESALGRSDKAISALSEEVNILKNLVGREVGLKHIYSGKVHVGMLEELKPEFLVLKLNNSSTKLKLSNVEILTDKELKDWKINNYPLRSYDVSAVFPESADPEVVVATVQGLEGVVDAEVMDTYSGSQIPEGSISITLRYLVFDDDAVLEVEKLLKGFGCQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBU5</accession>
    <name>F0TBU5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Flap endonuclease 1</fullName>
            <shortName evidence="11">FEN-1</shortName>
            <ecNumber evidence="11">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fen</name>
        <name evidence="14" type="ordered locus">Metbo_2068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645638.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2068"/>
    <dbReference type="EnsemblBacteria" id="ADZ10287">
        <property type="protein sequence ID" value="ADZ10287"/>
        <property type="gene ID" value="Metbo_2068"/>
    </dbReference>
    <dbReference type="GeneID" id="10278530"/>
    <dbReference type="KEGG" id="mel:Metbo_2068"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="OMA" id="GSQDYDS"/>
    <dbReference type="OrthoDB" id="41214at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_structure-sp_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="PTHR11081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 11 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPGN" evidence="13">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="XPGI" evidence="12">
        <location>
            <begin position="140"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="328" mass="37272" checksum="237DABA9675D9640" modified="2011-05-03" version="1">MGVKFKDIVTPENIKFQDLESKIVALDAANVIYQFLSSIRQVDGTPLMDENKNITSHFSGILYRTSSLIEKGIKPIYIFDGVSDALKKDTQDKRREVKEESQKKWDKALEEGNLEEARKYAVRSSRMSRDIIEGSKKLLEILGVPYIQALGEGEAQASYMVENGDAWCVGSQDYDCILFGATRMVRNLTITGGKANLELITLKKVLENMEITREQLVDIAILVGTDFNRGVKGVGAKTGLKLIKKHGDIFNVIEKNDYEMDVDPTILRNMFLEHNVVKDYELKWNSPDKEAAVEFLCDQHDFSEQRVLSALDKFAKLDNKQKSLEDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV1</accession>
    <name>F0TBV1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Box A-binding protein</fullName>
            <shortName evidence="6">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">TATA sequence-binding protein</fullName>
            <shortName evidence="6">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tbp</name>
        <name evidence="8" type="ordered locus">Metbo_2074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645644.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2074"/>
    <dbReference type="EnsemblBacteria" id="ADZ10293">
        <property type="protein sequence ID" value="ADZ10293"/>
        <property type="gene ID" value="Metbo_2074"/>
    </dbReference>
    <dbReference type="GeneID" id="10278536"/>
    <dbReference type="KEGG" id="mel:Metbo_2074"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="OrthoDB" id="83880at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="PTHR10126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="6">
        <location>
            <begin position="8"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="6">
        <location>
            <begin position="99"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="181" mass="19643" checksum="F70FF1E2E80FEC64" modified="2011-05-03" version="1">MTTVEIKVENIVASAALGKSLDLPQVAPALEGVEYNSEQFPGLVYKLKEPKTAALIFGSGKLVCTGAKSIEDSKKAIHIAVDKMRALDTEIPHEFEIKIQNIVASANLGKTLNLESVALDLENTEYEPEQFPGLVYRLDDPKVVLLLFGSGKVVCTGAKSFEDAQLGVEKTKERLAELDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY7</accession>
    <name>F0TBY7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="10">1.1.3.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="F0TBY7"/>
    <dbReference type="STRING" id="877455.Metbo_2110"/>
    <dbReference type="EnsemblBacteria" id="ADZ10329">
        <property type="protein sequence ID" value="ADZ10329"/>
        <property type="gene ID" value="Metbo_2110"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2110"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_6_0_2"/>
    <dbReference type="OMA" id="IPFIKPW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="8">
        <location>
            <begin position="2"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="115"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="419" mass="45943" checksum="0B5DD2ED817EA6DA" modified="2011-05-03" version="1">MRYICSYCNIYIYDEEKGDLETKLEPGTTLEEIPDSWLCPVCGMPKEYLKPVNNKIFEIKAKSYTETQHQTQDINYYRSIARNMLHGICGEYDVCNGKPDRMCSGQNFGGSIGFGGAGQGKTFEENYNSLQRYKLKMRVIKDHKEPEMSLSIFNKQIALPVMGSSLSGVKNSMNDSIPEETFYRGLLHGAMSSGTIGMVGNTNDVPDDLGVKTVGENHGWGIPIFKPQSQERLLELIKQAEELNVLAAGVDLEGAGSTFWKTAKKPVYRKGENDLIELVDSTELPLIFKGIMCREDAAKLLDVGAAACYVSNHGGRVLDGAQGVAEVLPEISSEVDGKIPVLADGAIRTGYDVLKILALGADVALIGRPLARLSLAGGEVPVKLYYKYVKDDLRNAMLLTGCDNLNEINMDILTGPSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC10</accession>
    <name>F0TC10_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Precorrin-8X methylmutase CbiC/CobH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645703.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2133"/>
    <dbReference type="EnsemblBacteria" id="ADZ10352">
        <property type="protein sequence ID" value="ADZ10352"/>
        <property type="gene ID" value="Metbo_2133"/>
    </dbReference>
    <dbReference type="GeneID" id="10278596"/>
    <dbReference type="KEGG" id="mel:Metbo_2133"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084703_1_1_2"/>
    <dbReference type="OMA" id="GAPIFCD"/>
    <dbReference type="OrthoDB" id="71138at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiC" evidence="4">
        <location>
            <begin position="23"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="23654" checksum="A2AAA694260ECB6A" modified="2011-05-03" version="1">MSENNDSGKATISMGASTKQGYEIAEKSREIVKSLINAKTKDLTPEEQSIVERIVHSTADPDYADITRISKNFVTESLRAIDQEKDILTDINMVKTGINSYSGRVDCYIRDERAVKIAKKEGITRAAAAIRVAAQEGFDGIVAVGNAPTALLEVLELHKTGEIKLNSVIGVPVGFVGAAESKQALKQTEIPYIITEGPKGGTPIAVATVNSLINLNKGNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC67</accession>
    <name>F0TC67_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644569.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0971"/>
    <dbReference type="EnsemblBacteria" id="ADZ09218">
        <property type="protein sequence ID" value="ADZ09218"/>
        <property type="gene ID" value="Metbo_0971"/>
    </dbReference>
    <dbReference type="GeneID" id="10277420"/>
    <dbReference type="KEGG" id="mel:Metbo_0971"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="OMA" id="CLPSIER"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="28"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="201"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="396" mass="44376" checksum="A5017350DDCD11FB" modified="2011-05-03" version="1">MRIGYFVGHFPYPNLVNTPDYINKYAHGGTEIAAYNLAVNMANRGNMVDVFTTSIDSKNSIELYPNMKVHRHGTSLKIASANPSIKLIYNPLNYDMDIVHAHSPIPYSDLPALLYAKRKKLPFVLTYQFDGVETGGSFARNAGVSLYNKLFIHRVLKSADTIIATTKSYAKESPFLRRYLDKIVVIPNGINIEEVTTPVTREKSREILGLPQDSEIILFFGSLVEYKGPDILLKAFKLVKNVFPTAKLIFAGRGHMDNELKETAKQMKIYDDVIFTGFVEDDEKPLYYKAADIFCLPSTTLAESFGIVNLEAMAAGLPIVSSDLGGIPDIVKNGVNGLLAKPYDFETVAKHLTKLLKNGEMREEMGQNGLKMVNNYTWDEVTTKTEKLYNKLIENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC99</accession>
    <name>F0TC99_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10366">
        <property type="protein sequence ID" value="ADZ10366"/>
        <property type="gene ID" value="Metbo_2151"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2151"/>
    <dbReference type="HOGENOM" id="CLU_1607169_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="165" mass="19716" checksum="3EADCE8285A20E6A" modified="2011-05-03" version="1">MVIKMNDEIRNDFNHKGVVYQAFNTKSSINVSEWKCYIGATEGMLKEAMAEHKQCSTDSSFHNAIREYGFDAFKWEIIHFKEDWETMEDLENLRRQKNTWYVYQAPHQIYIDSENDYYQKGITHRTHQKMLNVSPKPKNKFVLKNDGLNDEEVQHQLSYLEKQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB0</accession>
    <name>F0TCB0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10377">
        <property type="protein sequence ID" value="ADZ10377"/>
        <property type="gene ID" value="Metbo_2162"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2162"/>
    <dbReference type="HOGENOM" id="CLU_3113066_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="50" mass="5759" checksum="3AC764D09F492562" modified="2011-05-03" version="1">MKVDSQKCVLCDNAVTSPEEGWKFTNFNICHDCIERIFKDMSFVRGGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE1</accession>
    <name>F0TCE1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645759.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2194"/>
    <dbReference type="EnsemblBacteria" id="ADZ10408">
        <property type="protein sequence ID" value="ADZ10408"/>
        <property type="gene ID" value="Metbo_2194"/>
    </dbReference>
    <dbReference type="GeneID" id="10278657"/>
    <dbReference type="KEGG" id="mel:Metbo_2194"/>
    <dbReference type="eggNOG" id="arCOG01322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="OMA" id="QLMTNTH"/>
    <dbReference type="OrthoDB" id="104564at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="16803" checksum="0E3967B802F2802A" modified="2011-05-03" version="1">MKMGICDTTFARFNMAGAAMDQIKSQVANIKFVERTVPGVKDLPVASKKLIEEEGCELVMAFGMPGPEDIDKVCAHEASTGLIQAQLMTNTHIIEVFVHEDEGIDDKDLKVLAENRAREHADNVVKMLFKPKKMKREAGTGMREGRENKGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI8</accession>
    <name>F0TCI8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpiA</name>
        <name evidence="3" type="ordered locus">Metbo_1018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644616.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1018"/>
    <dbReference type="EnsemblBacteria" id="ADZ09265">
        <property type="protein sequence ID" value="ADZ09265"/>
        <property type="gene ID" value="Metbo_1018"/>
    </dbReference>
    <dbReference type="GeneID" id="10277467"/>
    <dbReference type="KEGG" id="mel:Metbo_1018"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_0_2"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="OrthoDB" id="98657at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="27"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="24373" checksum="DB8B1C7FC695FDD6" modified="2011-05-03" version="1">MITEIKRKVGYLAAEQIKDGDVVGLGTGSTTHFFIEKLGKRIVDEELKLLGIPTSYQSFFLAKDARIQITTLEEHDVDIAVDGADEVDPKFNLIKGGGAAHTLEKIVDSSASKFVVIVDGSKMVDKLGNFPVPVEVIPQASRTVRDQLIKMGGSPTMRMAERKDGPVVTDNGNFIYDVKFSVDNPKLLEVELNALPGVVENGIFTDIVDEVLVGTEKGVETLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP0</accession>
    <name>F0TCP0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase</fullName>
            <ecNumber evidence="2">6.3.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645781.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2216"/>
    <dbReference type="EnsemblBacteria" id="ADZ10430">
        <property type="protein sequence ID" value="ADZ10430"/>
        <property type="gene ID" value="Metbo_2216"/>
    </dbReference>
    <dbReference type="GeneID" id="10278682"/>
    <dbReference type="KEGG" id="mel:Metbo_2216"/>
    <dbReference type="eggNOG" id="arCOG02819">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_560971_0_0_2"/>
    <dbReference type="OMA" id="FKMMSGI"/>
    <dbReference type="OrthoDB" id="18097at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="117"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="470" mass="52124" checksum="CF5105CDC413F272" modified="2011-05-03" version="1">MKCLVIGAGNAGRPVARILNYVGNSILMTDRKKMNEFPQKVQETLKIMENEGVELAFDFDNFESLKSEEIEYAYVAPTVPLNAPVWEFLAEKNIEVLSNEYISDIINKQLEIDTIGITGTLGKTSTTHTVAEIFRAAGYNVWSCSSRMGNLLSEVIVDGIVNGLHKSNDVAVLELPHGTSRLMSKVELKVGIITNIFPDHLDEFDYSMEKYVERKLFITQSSEILISGIQCKENIESFRDDVIYYCHEHELCKVQGTLENGLLNINYNIPELGLEGNFNTPFKLRGYYFDNSIGAAAAALAYGISEESIKKGLSNFKGIEGHMEYIGNYKDREVHFDAAFVPAGLINTLESFDSENLVVVVDNPDSTNPRDKVKVGEVIGKYAKVIICSGYNETTKKFDVKAANDVIKGAQKSPAIKLATEDMFVAGELAIKHSEPGDIVVHVGPGAITNYDDLKFKMMSGIKEGCKKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU7</accession>
    <name>F0TCU7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Magnesium chelatase</fullName>
            <ecNumber evidence="6">6.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645838.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2273"/>
    <dbReference type="EnsemblBacteria" id="ADZ10487">
        <property type="protein sequence ID" value="ADZ10487"/>
        <property type="gene ID" value="Metbo_2273"/>
    </dbReference>
    <dbReference type="GeneID" id="10278739"/>
    <dbReference type="KEGG" id="mel:Metbo_2273"/>
    <dbReference type="eggNOG" id="arCOG00438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016684_6_2_2"/>
    <dbReference type="OMA" id="YYHLPKA"/>
    <dbReference type="OrthoDB" id="59101at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="526"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="341"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="711" mass="78712" checksum="17B5F83CC14D664C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z1</accession>
    <name>F0T5Z1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644735.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09384">
        <property type="protein sequence ID" value="ADZ09384"/>
        <property type="gene ID" value="Metbo_1140"/>
    </dbReference>
    <dbReference type="GeneID" id="24964536"/>
    <dbReference type="KEGG" id="mel:Metbo_1140"/>
    <dbReference type="eggNOG" id="arCOG10883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG10898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500294_0_0_2"/>
    <dbReference type="OrthoDB" id="100653at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="179" mass="19654" checksum="24F58266F733E3EA" modified="2011-05-03" version="1">MRLYFKYIIIGFIIAATTSFVLGYFSSILGGLVVGYLIADDYMDGAINGAIASAIVGLLYGVFYLLLFSRIFNTYGVSGGFEYVGIIFIAIAAIFAGLILGGIGGAAGVFIKEQSEIRNMQQNGVTSRKEDDGYLVCTNCNAYYKLQPNESPEDFNDECECGGKFRYYSNIDWLSKEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-02-10" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z7</accession>
    <name>F0T5Z7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645855.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2290"/>
    <dbReference type="EnsemblBacteria" id="ADZ10504">
        <property type="protein sequence ID" value="ADZ10504"/>
        <property type="gene ID" value="Metbo_2290"/>
    </dbReference>
    <dbReference type="GeneID" id="10278756"/>
    <dbReference type="KEGG" id="mel:Metbo_2290"/>
    <dbReference type="eggNOG" id="arCOG02245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036918_2_2_2"/>
    <dbReference type="OMA" id="AYGKRTF"/>
    <dbReference type="OrthoDB" id="118524at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="PTHR13353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00297">
        <property type="entry name" value="TIGR00297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="23421" checksum="FCE467211E8705F9" modified="2011-05-03" version="1">MINPLEYVVLIVVLGLVVYLKKALDLFGSIFMIVMGIIIIFSAGANWLMLIFLFLILGLLATKYKHEYKKKIGVYEGTRTLKNVISNGIVAFVMAAFGNYGGFIGSIATATADTLASEVGVVKQPRLITTLKKVPPGTDGGISLVGTAAGIIGAGIIGVAAYLLGIYPDPFVTLKISLVAGTVGCFVDSILGAVLERRDYISNEYVNLIATITGAAIGILMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T693</accession>
    <name>F0T693_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphopantothenate synthetase</fullName>
            <shortName evidence="1">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4-phosphopantoate + ATP + beta-alanine = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644759.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1164"/>
    <dbReference type="EnsemblBacteria" id="ADZ09408">
        <property type="protein sequence ID" value="ADZ09408"/>
        <property type="gene ID" value="Metbo_1164"/>
    </dbReference>
    <dbReference type="GeneID" id="10277614"/>
    <dbReference type="KEGG" id="mel:Metbo_1164"/>
    <dbReference type="eggNOG" id="arCOG04262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="OMA" id="PKSHPRY"/>
    <dbReference type="OrthoDB" id="76877at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="PTHR40695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="27363" checksum="8F45224EC48BFD12" modified="2011-05-03" version="1">MIHKDHPRYESLRLRAKIVDAYKNGILADSGMIAHGRGEAFDYLLGEQSTENAKLAVEAAAAALLLAEHPIISVNGNTAALVPDKIVELSNELDIPIEINLFYRTSDRVLKIEKLLRDHGAKTVLGTDHEKKALIKGLEGPRSSSSAEGIYKADVVLVPLEDGDRAEALVKNGKLLITIDLNPLSRTAKTSSITIVDNIVRAIPNLIDTVINLSQTDKVTLHQIISNFDNKENIQSSLKKISKKYTGEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B8</accession>
    <name>F0T6B8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644784.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09433">
        <property type="protein sequence ID" value="ADZ09433"/>
        <property type="gene ID" value="Metbo_1189"/>
    </dbReference>
    <dbReference type="GeneID" id="10277639"/>
    <dbReference type="KEGG" id="mel:Metbo_1189"/>
    <dbReference type="eggNOG" id="arCOG06457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163810_0_0_2"/>
    <dbReference type="OMA" id="ANYTPVY"/>
    <dbReference type="OrthoDB" id="80359at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025101">
        <property type="entry name" value="DUF4012"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13196">
        <property type="entry name" value="DUF4012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="238" mass="25102" checksum="2ABF4A3AF5CEFA0C" modified="2011-05-03" version="1" precursor="true">MSRKIIALIIILVAIGISAGYVVYSNLYQPGTTNVMKGDHTILLMTADPSEKRPGIGAVDMAFAIGVHDGDITNLTPIYPGGMVSATAMEPAEANAGGGHLRLHDSLWGADTEADVKNSQEIVQTNTGIKTDAVVIVTPEAVDAILNSIAPLNIPGYTNDTNASIDYIRSVSEGKNSTMTRGEASEVLMKPVMAAIKDPSKSGSLFQTVVQQYLKGNIIVIPKSLMTQFALSKGLSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C3</accession>
    <name>F0T6C3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644789.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1194"/>
    <dbReference type="EnsemblBacteria" id="ADZ09438">
        <property type="protein sequence ID" value="ADZ09438"/>
        <property type="gene ID" value="Metbo_1194"/>
    </dbReference>
    <dbReference type="GeneID" id="10277644"/>
    <dbReference type="KEGG" id="mel:Metbo_1194"/>
    <dbReference type="HOGENOM" id="CLU_2504961_0_0_2"/>
    <dbReference type="OrthoDB" id="140550at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="10048" checksum="84007AFFB5163476" modified="2011-05-03" version="1">MVNYATSEGFTFNMLMLKSFSLCDECSKLDVWEESKCSEKLNNLRNYRDNLLQFNFEDEFEIKQKDGMIKNLSAMITKLETLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E0</accession>
    <name>F0T6E0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-acetylhomoserine/O-acetylserine sulfhydrylase</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1212</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644806.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1212"/>
    <dbReference type="EnsemblBacteria" id="ADZ09455">
        <property type="protein sequence ID" value="ADZ09455"/>
        <property type="gene ID" value="Metbo_1212"/>
    </dbReference>
    <dbReference type="GeneID" id="10277663"/>
    <dbReference type="KEGG" id="mel:Metbo_1212"/>
    <dbReference type="eggNOG" id="arCOG00061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_2"/>
    <dbReference type="OMA" id="TYTLFAH"/>
    <dbReference type="OrthoDB" id="40508at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="439" mass="47927" checksum="7D2567885381BDCB" modified="2011-05-03" version="1">MSEQEKNNEWQGPGLSTIGLHVGQEEPDSATGSRAVPIYLTSSYVFNDTEHAANLFGLREFGNIYTRIMNPTTDVFEKRVAAVEGGATALGVASGMSAIFLAVLNVSELGENIVSGDNLYGGTYELFNYTLPRLGRTVKFVNSEKPEEFEAAIDEKTRAIYVESLGNPKLDVPDFEKLAKIAHDHDIPLIVDNTSTVGLLRPIDYGADITVISATKYIGGHGTSIGGVIVDSGKFNWGNGKFPQFTDPDPSYHGLKYWETFGDFPGAGNIAFTIRARVVLLRDLGPALSPFNAFQFLQGLETLELRVLKHAENALKVAKHLKNHPKVSWINYPGLEEDPRHEVASKYLKGGYGGLIGFGIEGGLESGKKFIENVELFSHLANIGDSKSLVIHPASTTHQQLTREEQETTGVTEDFVRLSIGLEDVEDIIADIDQALSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F0</accession>
    <name>F0T6F0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 1A</fullName>
            <shortName evidence="3">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif1a</name>
        <name evidence="9" type="ordered locus">Metbo_2371</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3 6">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645935.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2371"/>
    <dbReference type="EnsemblBacteria" id="ADZ10584">
        <property type="protein sequence ID" value="ADZ10584"/>
        <property type="gene ID" value="Metbo_2371"/>
    </dbReference>
    <dbReference type="GeneID" id="10278837"/>
    <dbReference type="KEGG" id="mel:Metbo_2371"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OMA" id="ADITWRY"/>
    <dbReference type="OrthoDB" id="102432at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="PTHR21668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="8">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11926" checksum="22601EF87947E06E" modified="2011-05-03" version="1">MTRGQNQSPQEVRRVRSPRRGEIPGVVEQILGHGKLKVRCADGKTRLSRIPGKMKKRIWIREGDVVLIKPWDFQSDEKADVIWRYTRTEANWLERRGYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N9</accession>
    <name>F0T6N9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 6">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nadE</name>
        <name evidence="9" type="ordered locus">Metbo_0019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4 6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643623.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0019"/>
    <dbReference type="EnsemblBacteria" id="ADZ08272">
        <property type="protein sequence ID" value="ADZ08272"/>
        <property type="gene ID" value="Metbo_0019"/>
    </dbReference>
    <dbReference type="GeneID" id="10276441"/>
    <dbReference type="KEGG" id="mel:Metbo_0019"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OMA" id="MAFLYDY"/>
    <dbReference type="OrthoDB" id="89944at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="277" mass="30518" checksum="B1A7EC7B0882A5B5" modified="2011-05-03" version="1">MKEKSIDIPMDDGEKTIKQLSEFIKDKVSQSRSAGVVIGLSGGIDSATTAYLCARALEKEQILGIIMPTETTSYEDIDDAVTVAEELGIEYEIIAVDDLISPFNELCMHSVSNKLKNTPSRSLANANLKARIRMMILYYHANDLGRMVVGTGNRTELLIGYFTKYGDGGVDILPIGNLYKTEVRSIAQILGVPENIIKKAPTAGLWHGQTDEEELGIKYETLDKLLFLMVDKGLDDQKIAENLDISVEEVLRIKNKVQSSKHKLEPAPTPGPDIGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q3</accession>
    <name>F0T6Q3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643637.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08286">
        <property type="protein sequence ID" value="ADZ08286"/>
        <property type="gene ID" value="Metbo_0033"/>
    </dbReference>
    <dbReference type="GeneID" id="10276455"/>
    <dbReference type="KEGG" id="mel:Metbo_0033"/>
    <dbReference type="eggNOG" id="arCOG10252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186520_0_0_2"/>
    <dbReference type="OMA" id="NRGRIYP"/>
    <dbReference type="OrthoDB" id="122506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="74" mass="8328" checksum="D228A539D67CA414" modified="2011-05-03" version="1">MAEKTELRQKVIGIGFEDDLIVKSQRGRTYSVKLAEDLEIDTEALFSKDTEEEVWATIDTGANPWVVLEVEITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V9</accession>
    <name>F0T6V9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1237</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644830.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1237"/>
    <dbReference type="EnsemblBacteria" id="ADZ09479">
        <property type="protein sequence ID" value="ADZ09479"/>
        <property type="gene ID" value="Metbo_1237"/>
    </dbReference>
    <dbReference type="GeneID" id="10277688"/>
    <dbReference type="KEGG" id="mel:Metbo_1237"/>
    <dbReference type="eggNOG" id="arCOG02386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068427_0_0_2"/>
    <dbReference type="OMA" id="CIERSER"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="136"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="207"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="296" mass="34338" checksum="D91423C8EB3C4F98" modified="2011-05-03" version="1">MRDGNIRILLVEDNPGDAVIIREMLKEIYKNKFELIHFQRLEDSVEHVIEDFDIVLLDLNLPDSRGIETFNIMNSHAPEMPIIILTGLLDEELAINIVGEGAQDYLVKGNIDKQLLSRSIKYSIERKRIEMDLRKSEEKYRAMVEKIESGLFLINSKNEINYVNKQMADMLGYNMDEILNRDVFSFIKKNCRAVFKKHLQSKARVGQTYELQFLKNNGSPLWVLMSTSPLKKMNGEYIGAISIMTDITARKGMEKSIMDAMIEKDKDFFFIMGNMVEAVKPLIQKDYVEDFQDKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T705</accession>
    <name>F0T705_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644876.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1283"/>
    <dbReference type="EnsemblBacteria" id="ADZ09525">
        <property type="protein sequence ID" value="ADZ09525"/>
        <property type="gene ID" value="Metbo_1283"/>
    </dbReference>
    <dbReference type="GeneID" id="10277734"/>
    <dbReference type="KEGG" id="mel:Metbo_1283"/>
    <dbReference type="eggNOG" id="arCOG04833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253609_0_0_2"/>
    <dbReference type="OMA" id="EMFRTPG"/>
    <dbReference type="OrthoDB" id="75706at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017059">
        <property type="entry name" value="NiFe-hyd_3_EhaH_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10125">
        <property type="entry name" value="NADHdeh_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036536">
        <property type="entry name" value="EhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="24209" checksum="16C97395A3829225" modified="2011-05-03" version="1">MDITILGSQIIGIIPLGDIVFYFTQFNLFMFATALAFTAIIAISKTETQIEAEFGKLGDRAVTVAKKEFKIRRFLAIICGLATAGAMITGDLFDFTLFVCLTGIVNIGIVGAVKQVDVLDAAYQYGLIAMMASLPLFGGAALILAATGTLSLLDINSLTYTTPMMILGSILLFLGVAGETGVAPFFATKAEMFRTPGSPFLLIIHLSSLLILVRLIEILLIINKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T723</accession>
    <name>F0T723_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase central domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646008.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2444"/>
    <dbReference type="EnsemblBacteria" id="ADZ10657">
        <property type="protein sequence ID" value="ADZ10657"/>
        <property type="gene ID" value="Metbo_2444"/>
    </dbReference>
    <dbReference type="GeneID" id="10278916"/>
    <dbReference type="KEGG" id="mel:Metbo_2444"/>
    <dbReference type="eggNOG" id="arCOG04163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_3_2"/>
    <dbReference type="OMA" id="FGEWAPK"/>
    <dbReference type="OrthoDB" id="41252at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="156"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="373" mass="42211" checksum="7FCE84CC3D53AB23" modified="2011-05-03" version="1">MKLNNITPDPLVSDKKSLKNINEPNEDAKLVVLQSIGYPFLCNLVENPKIEIFDKELFELYAKEQWEGSVVYEGSFLFDQKLLPDFAFKIVKAHPDNSKITSKTSILLVEVPESAEIKKIESSVKMEDVIGQERAKTKCKIITMYLKEPEKFKEWAPRNVLFHGTPGTGKTMLAKSLSNELNVPLYLVKATSLIGEHVGDGARQIHELFEMAAANAPSVIFIDEMDAIGLDRKFQSLRGDVSEVVNALLTEMDGIDHHNGVVTIGATNNPHLIDFAIRSRFEEEIEFTLPDEKDRKAILELYIETLPLKTDFNIEKLVKCTKGMSGRDIKDRVLKVALHKAIFEDQEIITWGHCKYALKQHKIKLNEPKGMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T760</accession>
    <name>F0T760_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646045.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2481"/>
    <dbReference type="EnsemblBacteria" id="ADZ10694">
        <property type="protein sequence ID" value="ADZ10694"/>
        <property type="gene ID" value="Metbo_2481"/>
    </dbReference>
    <dbReference type="GeneID" id="10278955"/>
    <dbReference type="KEGG" id="mel:Metbo_2481"/>
    <dbReference type="eggNOG" id="arCOG14021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891447_0_0_2"/>
    <dbReference type="OMA" id="LINWIVV"/>
    <dbReference type="OrthoDB" id="105280at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15171" checksum="CA7B4AE538D04B35" modified="2011-05-03" version="1">MKIRGDFVTNSSSTSFILTAKEDIFDVNIEHFTKTGKIGVVQLLNFLKNEMKNEGKQAKIGDNEFYFTQKKFLIGKSIKLNENIDPDEIASTDFSKLSDEELWNLINWIVVKGKGKDLYGIGATQIIDPKCECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T766</accession>
    <name>F0T766_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formylmethanofuran dehydrogenase subunit E region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646051.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2487"/>
    <dbReference type="EnsemblBacteria" id="ADZ10700">
        <property type="protein sequence ID" value="ADZ10700"/>
        <property type="gene ID" value="Metbo_2487"/>
    </dbReference>
    <dbReference type="GeneID" id="10278961"/>
    <dbReference type="KEGG" id="mel:Metbo_2487"/>
    <dbReference type="eggNOG" id="arCOG00762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087508_0_0_2"/>
    <dbReference type="OMA" id="HGHVCPY"/>
    <dbReference type="OrthoDB" id="90623at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FmdE" evidence="5">
        <location>
            <begin position="14"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DksA C4-type" evidence="4">
        <location>
            <begin position="148"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="187" mass="20846" checksum="7DB6F7B756786929" modified="2011-05-03" version="1">MSEYRGLLEMAGKFHGDICGGIVMGTKLAIHGMEKMQMKPGEKDKRLIVYTEIDRCISDAIMSVTQTSLGKKSLKPMGYGKFAATFVNIETGEALRLVDREANNKDERADDETIEELIERIDGTPGEELFDVQRVSVKIDKNDLPGKPLEIMKCKECGETVMDGKHHIKGGYAYCTSCYTGSYYTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F2</accession>
    <name>F0T7F2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="5">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0128</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643732.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0128"/>
    <dbReference type="EnsemblBacteria" id="ADZ08381">
        <property type="protein sequence ID" value="ADZ08381"/>
        <property type="gene ID" value="Metbo_0128"/>
    </dbReference>
    <dbReference type="GeneID" id="10276553"/>
    <dbReference type="KEGG" id="mel:Metbo_0128"/>
    <dbReference type="eggNOG" id="arCOG04295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060471_0_2_2"/>
    <dbReference type="OMA" id="VEAYHHT"/>
    <dbReference type="OrthoDB" id="70275at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="PTHR10429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="202" mass="22675" checksum="8C8FEF947F471321" modified="2011-05-03" version="1">MKLKETFYLKPTLTVARDLIGCVLVRKDSYGITSGKIVETEAYMGFEDKGSHSYNKRHTPSMDPLYKSGGHGYIFPIYGVGYCFNVVTEAVNVPRAVLIRALEPVEGKHLMGKRRHFSGINSSNLKNLTNGPVKLCQAMHIDATINGINLCGDELFILKGSKLRSNEKILTSARINIDYAEDDKFKPWRFILEGNKFLSRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F3</accession>
    <name>F0T7F3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-3-methyladenine glycosylase I</fullName>
            <ecNumber evidence="1">3.2.2.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.20" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643733.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0129"/>
    <dbReference type="EnsemblBacteria" id="ADZ08382">
        <property type="protein sequence ID" value="ADZ08382"/>
        <property type="gene ID" value="Metbo_0129"/>
    </dbReference>
    <dbReference type="GeneID" id="10276554"/>
    <dbReference type="KEGG" id="mel:Metbo_0129"/>
    <dbReference type="eggNOG" id="arCOG06523">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083758_1_0_2"/>
    <dbReference type="OMA" id="YVAYHDT"/>
    <dbReference type="OrthoDB" id="77102at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="21535" checksum="AFFAC288A3164556" modified="2011-05-03" version="1">MKTRCSWVDDDPMMKGYHDTEWGVPVHDDRKLFEFLILEGAQAGLSWKSILSRRENYKKAFDNFEPQIVAQYDESKLKELKEDPGIIRNKLKIKSAVTNALAVLDIQEEFGSLDAYLWQFVGGVTIQNHWKNINELPATTVESENMSRELKKRGFKFVGPTICYSFMQAVGMVNDHTVDCFRYKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F7</accession>
    <name>F0T7F7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron sulfur domain-containing, CDGSH-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643737.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08386">
        <property type="protein sequence ID" value="ADZ08386"/>
        <property type="gene ID" value="Metbo_0133"/>
    </dbReference>
    <dbReference type="GeneID" id="10276559"/>
    <dbReference type="KEGG" id="mel:Metbo_0133"/>
    <dbReference type="eggNOG" id="arCOG03174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1233392_0_0_2"/>
    <dbReference type="OMA" id="CDHSRFC"/>
    <dbReference type="OrthoDB" id="72436at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043231">
        <property type="term" value="C:intracellular membrane-bounded organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010693">
        <property type="entry name" value="Divergent_4Fe-4S_mono-cluster"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016548">
        <property type="entry name" value="UCP009180"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06902">
        <property type="entry name" value="Fer4_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009180">
        <property type="entry name" value="UCP009180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZnF_CDGSH" evidence="3">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="ZnF_CDGSH" evidence="3">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="27442" checksum="FA7CBC1C100546FA" modified="2011-05-03" version="1">MSENKKEPRIRIIKDGPYIVSGGVPLLKKGIGVDDEGNSYKWTLEVKYPNKESYSLCRCGKSKNKPFCDGTHLKTGFDGTETASRKPYMSEAVETTGPELTLTDVWPLCDHSRFCQRSGGIRELVAKSDDPESKKLAIEEGHNCPSGRLVIWDNETGEALEPEFEQSIVMIHDPLKKCEGPIWVRGGIPVESYDGHEYEIRNRITLCQCGKSENKPFCDGSHWLTKDQMEEWRKKWDEKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H0</accession>
    <name>F0T7H0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643750.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0147"/>
    <dbReference type="EnsemblBacteria" id="ADZ08399">
        <property type="protein sequence ID" value="ADZ08399"/>
        <property type="gene ID" value="Metbo_0147"/>
    </dbReference>
    <dbReference type="GeneID" id="10276573"/>
    <dbReference type="KEGG" id="mel:Metbo_0147"/>
    <dbReference type="eggNOG" id="arCOG00216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="OMA" id="MTGAMVP"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="184"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="404" mass="43980" checksum="DF362A6892A2AB7C" modified="2011-05-03" version="1">MFLTNLMPVQEAHDIIKSTLKTVGTENIVLEDAHMRVLAEDIRSNLNSPPFERSAMDGYAIKAEDTFGFSEANPAKFKIIDTIGAGQLSNKIVKTGQAVKISTGAPIPEGANAVVMEEYTNSDSELLEVETSVTPRENVSPMGEDFKKDDLVLRSGRLLKPQDLAIIASSGFNTVEVYKKPKVGIITTGTELVMPKPLLKGAEVINSNHYTFKAVVEASLAVPSLVHCIDSEKIVKDQFESMLDSHDVLITTGGTAISKGDVVVDVTEKLGEVLIHGVALRPGKPFGFGTVDDKPVFMLSGYPVAAMVQSDVFLRNSLYQMQGIEYQPQYVIKTTSKKIASNLGRTDYIRAKTDGSMVRPLKIKGSGIIRSMVESDSYILIEENLEGINEGTKCNVILYDSLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J1</accession>
    <name>F0T7J1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644910.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1317"/>
    <dbReference type="EnsemblBacteria" id="ADZ09559">
        <property type="protein sequence ID" value="ADZ09559"/>
        <property type="gene ID" value="Metbo_1317"/>
    </dbReference>
    <dbReference type="GeneID" id="10277768"/>
    <dbReference type="KEGG" id="mel:Metbo_1317"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="OMA" id="HEVWGDY"/>
    <dbReference type="OrthoDB" id="75045at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="198"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="379" mass="43536" checksum="D8D5EA8F68A9E5B6" modified="2011-05-03" version="1">MKIAFIYDAAYPWVKGGAERRIYELSTRLVKQGHEVHWYSLAWWREERKLMDIEMDGIKLHGVSKPKPLYTEDRRSIKEAIFFSLHLINPIMKEKFDVVDCQGFPFFSSFVCKFHQAIGRSKMVITLHEVWGDYWYEYLGRAGIFGKLVERSMLALTDRFITVSQKTERDLQAIKRTDKSHVVPNGIDLNQITNVKPAETGFELLFAGRLIKEKKVDLLLRSLPKVLKTCPDIKCMVVGDGPERSRLEGLCKELELTATVEFSGFMEGYNELISIMKSSEVLVLPSEREGFGMVVVEANACGTPVVVVDYPMNAAKDLVQEGRNGYISKPDPEDLAQTIIKTLKNRKNMVETSRSMSKNYDWDRIVDELENVYLQTQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N3</accession>
    <name>F0T7N3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="2">ADH synthase</shortName>
            <shortName evidence="2">ADHS</shortName>
            <shortName evidence="2">ADTH synthase</shortName>
            <ecNumber evidence="2">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aroA'</name>
        <name evidence="3" type="ordered locus">Metbo_1361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644952.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1361"/>
    <dbReference type="EnsemblBacteria" id="ADZ09601">
        <property type="protein sequence ID" value="ADZ09601"/>
        <property type="gene ID" value="Metbo_1361"/>
    </dbReference>
    <dbReference type="GeneID" id="10277812"/>
    <dbReference type="KEGG" id="mel:Metbo_1361"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="OMA" id="CEYWGMP"/>
    <dbReference type="OrthoDB" id="57391at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01949">
        <property type="entry name" value="AroFGH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="25"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="28207" checksum="20B9108D49E30296" modified="2011-05-03" version="1">MIGKRIRIERILNRKTGRCVIVPMDHGMSIGPVDGIIDMAETIDEVASGGANAVIEHKGMVGKGHRGYGNDIGLIIHLSASTSVGPDPDHKVLVTSVEKALKLGADAVSVHVNIGSEKEPEMLVQMGTIAETCDEWGMPLIAMMYPRGKKIENEHSVDVVKLAARAGAELGADIIKTNYTGDPQTFKEVVDGCPVPVVIAGGPMVETDKQLLEMVKNSVDVGGAGVAIGRNIFQAKSPSKTTRAIAEIVHNNMEVEDALKILNGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N5</accession>
    <name>F0T7N5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644954.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1363"/>
    <dbReference type="EnsemblBacteria" id="ADZ09603">
        <property type="protein sequence ID" value="ADZ09603"/>
        <property type="gene ID" value="Metbo_1363"/>
    </dbReference>
    <dbReference type="GeneID" id="10277814"/>
    <dbReference type="KEGG" id="mel:Metbo_1363"/>
    <dbReference type="eggNOG" id="arCOG00373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340750_0_0_2"/>
    <dbReference type="OMA" id="KVEWLDK"/>
    <dbReference type="OrthoDB" id="98209at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="23914" checksum="E1B94EAEB013E0C4" modified="2011-05-03" version="1">MDESNKQELDEEIKKLLNERNELNNDIRNLDWAKIIKLEKENEELQRKVEWLDKDKKRMEREKENLNRQVLNSRHKKWFNTVKMILILGVIDLLIIPLIITLLGLSILWIFLGIGVVTFFGTLIIANYMSGTGQFNSGEIRKAITTSVIVVYLIFIPLITFGSIQIPNDGTVKGIVQNFTWIVGIIVVFYFISRSIEEYGKAKNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N7</accession>
    <name>F0T7N7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HI0933 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644956.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1365"/>
    <dbReference type="EnsemblBacteria" id="ADZ09605">
        <property type="protein sequence ID" value="ADZ09605"/>
        <property type="gene ID" value="Metbo_1365"/>
    </dbReference>
    <dbReference type="GeneID" id="10277816"/>
    <dbReference type="KEGG" id="mel:Metbo_1365"/>
    <dbReference type="eggNOG" id="arCOG00575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025174_3_1_2"/>
    <dbReference type="OMA" id="RCNFTNM"/>
    <dbReference type="OrthoDB" id="23211at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="412" mass="44746" checksum="27157C243044C954" modified="2011-05-03" version="1">MNKIIDLAVVGAGPAGMMAAITASSNGNSVILIEKNNSAGKKLLITGNGRCNLTNTKPMNDFLKKFQKKGSFLRSAFTALTNNDLIDFFETRGLKLKVESDGRVFPVTDNSRDVLGILLKELKKQFVNIRYSTTVVSVHQDPQGFRLILDHGEDIMAKNVVLATGGVSYSATGSDGEGHKIAEETGHNITALEPGIVPVLTHEFWVRELSGLQLESAAAVIKHSKGKVKLEGDLLFTHQGLSGPMILNNSAKLVEILRRDERILLNIDIHPEKTVNQLSEEFLTAREEHGKVDLGNHMKLKLPNRMIPLFLEMAGADPKLKMNQLGKKERNNIINLLKSFPLTVTGTSSIDKAMVTCGGVSSKDFDPNTMESKTVTGLFFAGELVDGCGPSGGYNLQQAFSTGFLAGSKINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P5</accession>
    <name>F0T7P5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644964.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1373"/>
    <dbReference type="EnsemblBacteria" id="ADZ09613">
        <property type="protein sequence ID" value="ADZ09613"/>
        <property type="gene ID" value="Metbo_1373"/>
    </dbReference>
    <dbReference type="GeneID" id="10277824"/>
    <dbReference type="KEGG" id="mel:Metbo_1373"/>
    <dbReference type="eggNOG" id="arCOG00520">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067890_1_2_2"/>
    <dbReference type="OMA" id="MSCYKFI"/>
    <dbReference type="OrthoDB" id="87395at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024747">
        <property type="entry name" value="Pyridox_Oxase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12900">
        <property type="entry name" value="Pyridox_ox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="157" mass="18434" checksum="106BEA0849C6EAF6" modified="2011-05-03" version="1">MKIHKIPLMSKEEYDKFIKENYVSRIGFKGEYPYIAPFLYVFDGDFIYFMSTKYGKKIKRFQANPNVAVEIEKYEDDLSEYRFVTLQGRIEQVENIEEKLKVKKCFLNLIQNKSLSKNVLAALGHSPDDELESIVNEDRSYIWKLVDVKSIIGIKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R5</accession>
    <name>F0T7R5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643753.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0150"/>
    <dbReference type="EnsemblBacteria" id="ADZ08402">
        <property type="protein sequence ID" value="ADZ08402"/>
        <property type="gene ID" value="Metbo_0150"/>
    </dbReference>
    <dbReference type="GeneID" id="10276576"/>
    <dbReference type="KEGG" id="mel:Metbo_0150"/>
    <dbReference type="eggNOG" id="arCOG01470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697588_0_0_2"/>
    <dbReference type="OMA" id="DYSDPRR"/>
    <dbReference type="OrthoDB" id="40098at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="395" mass="43683" checksum="4D268EB7A621230E" modified="2011-05-03" version="1" precursor="true">MKFTTITKWELKNTLKSRKFMMIFVLQLSVLLLMIVVFNSFVANIESEKGISITPSLNGFANMDVQDQSGLISKYVNPEIISVNNVTSYNTSILRTKNGLTTGFLYVPGNADSIIKAGDTVNMLVYVDAADPKMSVVTDEANNTAKTIAQSYSSSLTMSANSQNQTANFTQETTGESLPLQIIKKVMLTILLFLPLLLFGNIIIDSIVGEKERKTGEILVAMPLSQSDIIVGKGLAVALTISLQVLIWLVVLIIFGFKIFNPLAVFLFVFLTSIPIIGVTSVVAAYSKNYKEAGIGLSLVYLVVVGFLIIPVLVYISRKSFMANISPMTIVMRLFSGDFIPLPDYLLCLGFIALVSLVSYWIAIKMFSRDDITFGPRPGIFRILFEFLTLKFFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W5</accession>
    <name>F0T7W5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase dimerization</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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            <strain evidence="5 6">AL-21</strain>
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        <source>
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        <source>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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            <strain evidence="2 3">AL-21</strain>
        </source>
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    </evidence>
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        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T816</accession>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <reference evidence="3 4" key="2">
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        </source>
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    </evidence>
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</entry>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="12463" checksum="4DEB27B0C2AB032F" modified="2011-05-03" version="1">MVEIKWKPVSIGLVIAIVIGLILGILTSWGDLLGYLIGSAVVAYLVGGTYKNGVIYGAIVGLVAAVVVLVVGLMGLSVAYTTNLFLVGWIAISTVIVLSIIIGAITGAIGGIIGIIIKNRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T829</accession>
    <name>F0T829_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="2">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="2">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">msrA</name>
        <name evidence="4" type="ordered locus">Metbo_1416</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645006.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1416"/>
    <dbReference type="EnsemblBacteria" id="ADZ09655">
        <property type="protein sequence ID" value="ADZ09655"/>
        <property type="gene ID" value="Metbo_1416"/>
    </dbReference>
    <dbReference type="GeneID" id="10277867"/>
    <dbReference type="KEGG" id="mel:Metbo_1416"/>
    <dbReference type="eggNOG" id="arCOG02816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_0_2"/>
    <dbReference type="OMA" id="AGPFYYA"/>
    <dbReference type="OrthoDB" id="86136at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PMSR" evidence="3">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18542" checksum="C64326E50B8E01F5" modified="2011-05-03" version="1">MADQEKYETASFAAGCFWGVEAAFRELKGVIETTVGYMGGHTKDPNYNEVCTGTTGHAETLQLKYDPDEISFEKLLEVFWNKHDPTTKNRQGPDIGEQYRSIIFYHNPEQKKLAEESKEKLDSSGKYRNPVVTEIIPAGTFYPAEDYHQQYLEKRGRKFCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T868</accession>
    <name>F0T868_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dnaJ</name>
        <name evidence="9" type="ordered locus">Metbo_0228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DnaJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643831.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0228"/>
    <dbReference type="EnsemblBacteria" id="ADZ08480">
        <property type="protein sequence ID" value="ADZ08480"/>
        <property type="gene ID" value="Metbo_0228"/>
    </dbReference>
    <dbReference type="GeneID" id="10276658"/>
    <dbReference type="KEGG" id="mel:Metbo_0228"/>
    <dbReference type="eggNOG" id="arCOG02846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_2"/>
    <dbReference type="OMA" id="DLHCTVT"/>
    <dbReference type="OrthoDB" id="50583at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="146"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="146"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="388" mass="42376" checksum="0004C10EFA21CB03" modified="2011-05-03" version="1">MAEKRDYYEVLGVEKGADKKEIKKAYRKLAMKYHPDVSEDEESGEKFKEISEAYAVLSDAEKRQTYDQYGHAGMNGFSQEDIFNNANFEDIFRGFGFGGGQGNRQSGGFESIFDLFGFGGGNRNGPVQGDDVYYEMSITLEEAASGLETDVEIPHKKTCPHCHGSKAEPGTNTKTCPECGGSGQVRQVSNTPLGQFATVRPCRTCRGEGQIIETPCTECHGRGIVNHQSTIHIKIPAGVEDGSRLRVPGEGDVGEKGGAPGDLYVIIGVKQHQLFHREGSDLVYNMPISFVQASIGATVEAPTLTGAVDLKIPAGTQTGTSFRIKGEGMPHLRWNGKGNLYVKVKIVTPKKLSPKQKELLVEFAEVSGEEIYIEDKGFFDKVKDAINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A0</accession>
    <name>F0T8A0_METLA</name>
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        <submittedName>
            <fullName evidence="8">Multiple resistance and pH regulation protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643863.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0260"/>
    <dbReference type="EnsemblBacteria" id="ADZ08512">
        <property type="protein sequence ID" value="ADZ08512"/>
        <property type="gene ID" value="Metbo_0260"/>
    </dbReference>
    <dbReference type="GeneID" id="10276692"/>
    <dbReference type="KEGG" id="mel:Metbo_0260"/>
    <dbReference type="eggNOG" id="arCOG04878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189621_0_0_2"/>
    <dbReference type="OMA" id="FCRDIAY"/>
    <dbReference type="OrthoDB" id="126945at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="25"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9146" checksum="6DDCAABF0AFFCAA0" modified="2011-05-03" version="1">MNILLISEYILMGALAIFALATIRITTRKKIAMALVGLSGLSISIAVMLILIQHIYNLAFCRDIATALVFLGPVGTIAFARVLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B4</accession>
    <name>F0T8B4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase (Ubiquinone), subunit 1 related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643877.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0274"/>
    <dbReference type="EnsemblBacteria" id="ADZ08526">
        <property type="protein sequence ID" value="ADZ08526"/>
        <property type="gene ID" value="Metbo_0274"/>
    </dbReference>
    <dbReference type="GeneID" id="10276706"/>
    <dbReference type="KEGG" id="mel:Metbo_0274"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830576_0_0_2"/>
    <dbReference type="OMA" id="FPFYLAT"/>
    <dbReference type="OrthoDB" id="88886at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="36306" checksum="6CAD36F9F9850E1D" modified="2011-05-03" version="1">MTAVNSLIAVIGTLLVALIVSVWLPGIERKFVHARIQQRVGPPISSAGIMAPLKFFFKQTINPNSPMPKLYNALPLVSLLSVIFILLFLMPQLYALAALTSIIAIVGLLKIEEVIYMFMGSLSKSIMSLNMPFPDIVKGAKHPNTTRTFMEELSSLRAFRLIAFGSFPLYIALFIPVALSKSIYLSGIVNYQQIHGPILFTMAGVLGAIVFFIGYMILLNEYPFVILKTKADVIEGPLMEYAAKYRAYVYITRGFLMFVLASLFTTLFLGIPPNLFSWNILITIAVALIFPILMAIMSAFSPIFTNKQFYPAVAGVSIIGILGIVVSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F6</accession>
    <name>F0T8F6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LemA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1471</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645058.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09707">
        <property type="protein sequence ID" value="ADZ09707"/>
        <property type="gene ID" value="Metbo_1471"/>
    </dbReference>
    <dbReference type="GeneID" id="10277922"/>
    <dbReference type="KEGG" id="mel:Metbo_1471"/>
    <dbReference type="eggNOG" id="arCOG04574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_0_1_2"/>
    <dbReference type="OMA" id="YNNAVQA"/>
    <dbReference type="OrthoDB" id="78720at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="189" mass="21759" checksum="CC7836F65F9C8D9A" modified="2011-05-03" version="1">MDVSYIILGIIILIVIYIVYSYNRLIAMRNMVDTAYSNIDTLLQKRFDLVPNLVNTVKGYMTHERELLEEVSRARSDWMTASTIQENAGADNVAASALKSIFAVAENYPDLKANKNFLLLQEELVGIENKIAYARQRYNRTVLDLNNAVQQFPTNITAQFFKFQTREYFEVESNKIREAPNVKIFDGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L0</accession>
    <name>F0T8L0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, HxlR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645112.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1531"/>
    <dbReference type="EnsemblBacteria" id="ADZ09761">
        <property type="protein sequence ID" value="ADZ09761"/>
        <property type="gene ID" value="Metbo_1531"/>
    </dbReference>
    <dbReference type="GeneID" id="10277986"/>
    <dbReference type="KEGG" id="mel:Metbo_1531"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_3_2"/>
    <dbReference type="OMA" id="VIYELAV"/>
    <dbReference type="OrthoDB" id="102459at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="15300" checksum="A97DC7A5A237FCAA" modified="2011-05-03" version="1">MGNDPYLNKIYQTIDDTFGYLRKKWNIQIIKGLFCDCKHFKDFLEQHPTLSSKVLAERLKELEEEGIIEKKSVNSTQTEYCLTEKGLRLNKIIYEMFDFALDEVMKDEKSSKQKAESREYLNKCLLTGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L6</accession>
    <name>F0T8L6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pre-mRNA processing ribonucleoprotein, binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643909.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0306"/>
    <dbReference type="EnsemblBacteria" id="ADZ08558">
        <property type="protein sequence ID" value="ADZ08558"/>
        <property type="gene ID" value="Metbo_0306"/>
    </dbReference>
    <dbReference type="GeneID" id="10276738"/>
    <dbReference type="KEGG" id="mel:Metbo_0306"/>
    <dbReference type="eggNOG" id="arCOG01923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015495_1_0_2"/>
    <dbReference type="OMA" id="REWYSLH"/>
    <dbReference type="OrthoDB" id="89321at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D RNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="PTHR10894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="SSF89124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0543">Viral nucleoprotein</keyword>
    <keyword evidence="4" id="KW-0946">Virion</keyword>
    <feature type="domain" description="Nop" evidence="3">
        <location>
            <begin position="254"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="412" mass="47032" checksum="E1A985B7E590F5E6" modified="2011-05-03" version="1">MKCYVTYSFAGFICLDENCVVLDYELFPREKLIERIGKIDAGNLSIEEEFLLKRMVDNYDSVVIETSLPHSKYSHLKESNKFEFETPHLGGEFFRSNMEETLLTVGFLDEDSDLRSILQQVSIDLTNNKIRKASESEDMFLIQAINSIEELDETISKLVERLREWYAIHFPEMDGIKNHERYAELVSEFGDRETIINSGTLSEDINPKFVSESVGATISEPDLKMVMEFASSIHSLQTTKKSLNTYVDERMGEIAPNLRELAGASLGAKLIAHVGGVEKLSKMPSGTVQVLGAEKALFRHLKTGERPPKHGLIFQHPSVRGAKWWLRGKIARTLALKISLAVRKDVYSGDYDPEIVKNYEKRVEEITKDNPFPKRSKKPDKFGDKDAKSGKNSGKKKKKKRDKYKKNIKDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M5</accession>
    <name>F0T8M5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643918.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0315"/>
    <dbReference type="EnsemblBacteria" id="ADZ08567">
        <property type="protein sequence ID" value="ADZ08567"/>
        <property type="gene ID" value="Metbo_0315"/>
    </dbReference>
    <dbReference type="GeneID" id="10276752"/>
    <dbReference type="KEGG" id="mel:Metbo_0315"/>
    <dbReference type="eggNOG" id="arCOG00622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982154_0_0_2"/>
    <dbReference type="OMA" id="GRIMFRT"/>
    <dbReference type="OrthoDB" id="46801at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016427">
        <property type="entry name" value="UCP004699_CBS/ParB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004699">
        <property type="entry name" value="UCP004699_CBS_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="29861" checksum="4C2EB5FA04C7AB4A" modified="2011-05-03" version="1">MTSSALVKDYMTKEVITVNPETPNEEVIMLMKGTGHDGFPVKTNGEVIGMVTAFDLLLKEWLPLVKDIMSTDIVVAEEDMSINDAARVMFRMGISRLPVINKNSKLVGILTNTDIVRSHIERSTPMKVDYFKRTLEQLYEIDTKVVRMKVPIDRIRPTQDKIYADELQGRTYEIKRGLAEPTIIAHTGDRYILVDGHHRTVAALKLGCKDIDSYVIQLDHDIHLGLEKTADKNGIFSFKDIEVIDDAQHPLIAITSSLRKEARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R5</accession>
    <name>F0T8R5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 19">Tetrahydromethanopterin S-methyltransferase subunit D</fullName>
            <ecNumber evidence="8 19">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mtrD</name>
        <name evidence="20" type="ordered locus">Metbo_0355</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AL-21</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 19">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 19">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 19">Cell membrane</location>
            <topology evidence="4 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the MtrD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="8 19"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643958.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0355"/>
    <dbReference type="EnsemblBacteria" id="ADZ08607">
        <property type="protein sequence ID" value="ADZ08607"/>
        <property type="gene ID" value="Metbo_0355"/>
    </dbReference>
    <dbReference type="GeneID" id="10276792"/>
    <dbReference type="KEGG" id="mel:Metbo_0355"/>
    <dbReference type="eggNOG" id="arCOG04869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109510_0_0_2"/>
    <dbReference type="OMA" id="HDPKFKR"/>
    <dbReference type="OrthoDB" id="95538at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01097">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005779">
        <property type="entry name" value="MeTrfase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04207">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016552">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01112">
        <property type="entry name" value="mtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 19" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="12 19 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 19" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ADZ08607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="24127" checksum="B7DB6E7D8B1D3CF1" modified="2011-05-03" version="1">MDPLLLIGAISVGGLLIGGGVHFIPVGGAPAAIATATGVGTGTAMLAAGGGMTGLIAAAAMTGQPLWLILASGAIGSMLMIGITMLVGNFIYVWGVGCVPASAKVEYDPITKYEQSKYVTSGTEGHGIPTVCFVSGIIGGALGGIGGGLIYWALYEGLSILPGYQLSALLGGGVSLTAATVAAIFAIGVFFINAVVASYNIGGTIEGFHDPKFKRIPRGALACLIVSIVTGLIAVLLLKGGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S6</accession>
    <name>F0T8S6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SufBD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643969.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0366"/>
    <dbReference type="EnsemblBacteria" id="ADZ08618">
        <property type="protein sequence ID" value="ADZ08618"/>
        <property type="gene ID" value="Metbo_0366"/>
    </dbReference>
    <dbReference type="GeneID" id="10276803"/>
    <dbReference type="KEGG" id="mel:Metbo_0366"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="OMA" id="SIQSYPT"/>
    <dbReference type="OrthoDB" id="19042at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="406" mass="44438" checksum="9ED0908D943252E3" modified="2011-05-03" version="1">MLQNTIERAEKAKKKKAALGEDIDIEEFEGEEAGEHEEVESLRDLPGKYQKSLLKVGVDPSETGRAGSFLQIDQSKVCSNCESENIELMGLPQALDKYDWVKDYMWNAVPVDTDKYTAKTALGEPGGYFIRSLPNTKEVFPIQACMFIGDEKVAQTAHNIIIAEENSELHIITGCSTGSDVQSAMHVGVSEFYLKKGAKITFTMVHNWAEQVEVRPRTGVILSDDTTYISNYILTSPVNTIQTYPTAYCQGENSKVVFQSILGGQKDSILDTGSRAILTGKNSSAEMITRAVSKDESQIYTRGHLAGKSPDVKGHLECMGLVLSDDSLIYSVPELEGSATDLELSHEAAVGKIAEEEVLYLMSRGLTEEEAASMIVKGFLSMDIAGLPPELAAETQRMIDLSIQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U5</accession>
    <name>F0T8U5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645124.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09773">
        <property type="protein sequence ID" value="ADZ09773"/>
        <property type="gene ID" value="Metbo_1543"/>
    </dbReference>
    <dbReference type="GeneID" id="10277998"/>
    <dbReference type="KEGG" id="mel:Metbo_1543"/>
    <dbReference type="eggNOG" id="arCOG10162">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080337_0_0_2"/>
    <dbReference type="OrthoDB" id="95382at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="31201" checksum="F75DD22E8A805505" modified="2011-05-03" version="1">MDIKEIKSFELTPFTKMSASIYGVLGFVAAVVILVAMVIVQVTGVASEIGSFNVITGIGIPLIILLPILGFFSTIAVSFFSVILYNALVPRLGGIKLGMNGNEIEKIPEVSFALILAAISAIWAFIVGLTLAAVASPILSIIGSTPDATGIAANFTNITGSAIPTGTNFGAIGIILSVALIIGLPILAFVLTFISNAFFAIFYNYIVTRVAKINIEFASIRESLHEIKHIPVLPTALAVALVYLIMGIITGLLSHNYTEFISNFILYFIETALIAILYNYLAPKIGTVKLDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y3</accession>
    <name>F0T8Y3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645162.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09811">
        <property type="protein sequence ID" value="ADZ09811"/>
        <property type="gene ID" value="Metbo_1584"/>
    </dbReference>
    <dbReference type="GeneID" id="10278041"/>
    <dbReference type="KEGG" id="mel:Metbo_1584"/>
    <dbReference type="eggNOG" id="arCOG14006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2420081_0_0_2"/>
    <dbReference type="OMA" id="NITIYEM"/>
    <dbReference type="OrthoDB" id="141026at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="98" mass="11235" checksum="72B0E9436B9A57FE" modified="2011-05-03" version="1">MVYIATKNELRELNKELVERIRAGECGEVNIHEMLKAVSVLDTTIEGQTYLIDHGTDEKFGELVDKLNNITHDMRDGKMNITDLTAKYTQDLPQEQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z0</accession>
    <name>F0T8Z0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1591</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645169.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1591"/>
    <dbReference type="EnsemblBacteria" id="ADZ09818">
        <property type="protein sequence ID" value="ADZ09818"/>
        <property type="gene ID" value="Metbo_1591"/>
    </dbReference>
    <dbReference type="GeneID" id="10278048"/>
    <dbReference type="KEGG" id="mel:Metbo_1591"/>
    <dbReference type="eggNOG" id="arCOG02276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_265168_0_0_2"/>
    <dbReference type="OMA" id="IYTYVSP"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="131"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="203"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="432"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="551"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="674"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="745"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="874"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="925"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="1163"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="534"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1257" mass="143761" checksum="98F8FB1F244677B2" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T910</accession>
    <name>F0T910_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="7">PEP synthase</shortName>
            <ecNumber evidence="7">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0380</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 7">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + pyruvate = AMP + 2 H(+) + phosphate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643983.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0380"/>
    <dbReference type="EnsemblBacteria" id="ADZ08632">
        <property type="protein sequence ID" value="ADZ08632"/>
        <property type="gene ID" value="Metbo_0380"/>
    </dbReference>
    <dbReference type="GeneID" id="10276817"/>
    <dbReference type="KEGG" id="mel:Metbo_0380"/>
    <dbReference type="eggNOG" id="arCOG01111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="OrthoDB" id="3139at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PTHR43030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="9">
        <location>
            <begin position="17"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="8">
        <location>
            <begin position="364"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="10">
        <location>
            <begin position="465"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="765" mass="84269" checksum="BE7043F257BE24F2" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T926</accession>
    <name>F0T926_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643999.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0396"/>
    <dbReference type="EnsemblBacteria" id="ADZ08648">
        <property type="protein sequence ID" value="ADZ08648"/>
        <property type="gene ID" value="Metbo_0396"/>
    </dbReference>
    <dbReference type="GeneID" id="10276833"/>
    <dbReference type="KEGG" id="mel:Metbo_0396"/>
    <dbReference type="eggNOG" id="arCOG00563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587455_0_0_2"/>
    <dbReference type="OMA" id="WDIFYHI"/>
    <dbReference type="OrthoDB" id="27401at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        <location>
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            <end position="379"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="527" mass="59669" checksum="265D14DDF4E767CD" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T988</accession>
    <name>F0T988_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="Metbo_1612"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DUF4013"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="267" mass="29562" checksum="254DC362F5918133" modified="2011-05-03" version="1">MDIGDIVKNSLGYPLSNKKNFLILGVIILFAEIYSIVQSFGFKSSIFGLFLILTLLLMIFRSGYNLRILNSSIGGYDVLPDFGNWMGMFKDGVKIWIVLIIFIIPLLILLFVFGFIISLILISSGASTSVLGKIMLMAILAIVGIYLIIVYPLILMALANMAKNENNISYALKLSEIRTKISEIGLGNYIGWYIVTGIIYAVIIIIGTTIIFAFGLGHVKFIGIIINALIIGPFASIFLYRSTSLMYRSVLELENETDSSEDPELTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T998</accession>
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        <submittedName>
            <fullName evidence="7">Multi-sensor signal transduction histidine kinase</fullName>
        </submittedName>
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    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_266158_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
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        <property type="entry name" value="His_kinase_dom"/>
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        <property type="entry name" value="HisK_dim/P"/>
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        <property type="entry name" value="HisK_dim/P_sf"/>
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        <property type="entry name" value="PAS-assoc_C"/>
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        <property type="entry name" value="PAS_fold"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="sensory_box"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
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        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
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            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="81"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="661"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="1031"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="934"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1246" mass="141369" checksum="6C5124BCE270740B" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B3</accession>
    <name>F0T9B3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645215.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09864">
        <property type="protein sequence ID" value="ADZ09864"/>
        <property type="gene ID" value="Metbo_1638"/>
    </dbReference>
    <dbReference type="GeneID" id="10278095"/>
    <dbReference type="KEGG" id="mel:Metbo_1638"/>
    <dbReference type="HOGENOM" id="CLU_1631666_0_0_2"/>
    <dbReference type="OrthoDB" id="136449at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18694" checksum="EAE1E112732B186F" modified="2011-05-03" version="1">MDNKTKKIFQKDFSILETKNSLLKEPENLKVNSEALGIGLPDIYSKLEKERVSSLKFEDIQHRIDILKSEENVDPEQIELLEEMKRASHLHNRADSNPTDEINGFKNVHDTVRAELESIGNLSGKSLSKESILTIQKQIDSDPLMFRSKYPELFDYMTSNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G8</accession>
    <name>F0T9G8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA decarbonylase/synthase complex subunit beta</fullName>
            <shortName evidence="9">ACDS complex subunit beta</shortName>
            <ecNumber evidence="9">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ACDS complex acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdhC</name>
        <name evidence="11" type="ordered locus">Metbo_0464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="9">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644067.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0464"/>
    <dbReference type="EnsemblBacteria" id="ADZ08716">
        <property type="protein sequence ID" value="ADZ08716"/>
        <property type="gene ID" value="Metbo_0464"/>
    </dbReference>
    <dbReference type="GeneID" id="10276902"/>
    <dbReference type="KEGG" id="mel:Metbo_0464"/>
    <dbReference type="eggNOG" id="arCOG04360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_613408_0_0_2"/>
    <dbReference type="OMA" id="FMHLNQR"/>
    <dbReference type="OrthoDB" id="26992at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACS_CODH_B_C" evidence="10">
        <location>
            <begin position="167"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="468" mass="52378" checksum="058CC5DE08A1ECD4" modified="2011-05-03" version="1">MFEDIPVDVSPMYEGERIRAANMFVELAGPKSIGAELVQVEEDVEDGKIEVIGPDLNGMTEGEIYPFGLLIQIKGEKLEKELEGVIERRTHELCNYIKGFMHLNQRDQIWCRVSKEAVESGFGFEHLGKAISILFKEEFPIIEAISIKVLTDREEVETFLDNASKQYEERDAKARELSDEDVDVFYGCVMCQSFAPTHTCVVTPDRTALCGAINWFDCRAAAKMDPDGPIFEIEKGEVLDDVKGEYVNVNTVMADKSQGTVDRVYLHSVFGYPHTSCGCFEAVAFYIPELDGIGIVDRDYKGETPLGIPFSAMAGQCSGGKQVEGFAGLSLEYMRSPKFLQADGGYERIIWLPKAIKDSLIEFIPEDLADKIPTEEEANSIKEIRKFLREHEHPVVERLKESKKSAAQDVETEEVVEETATGSEFMVDEAPMQQVGYVPELTVPSSGGMKIIFKNAKIYAEKVIIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I0</accession>
    <name>F0T9I0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644079.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0476"/>
    <dbReference type="EnsemblBacteria" id="ADZ08728">
        <property type="protein sequence ID" value="ADZ08728"/>
        <property type="gene ID" value="Metbo_0476"/>
    </dbReference>
    <dbReference type="GeneID" id="10276916"/>
    <dbReference type="KEGG" id="mel:Metbo_0476"/>
    <dbReference type="eggNOG" id="arCOG04237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070533_2_0_2"/>
    <dbReference type="OMA" id="GPYVALM"/>
    <dbReference type="OrthoDB" id="61183at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28869" checksum="B8AA728F2FC537BB" modified="2011-05-03" version="1">MITEELLKGMMKSQNPGWKTAITKVEHNHLSTRGYSQEDLIENYSFPEMVYLLLQGKIPSEKQKKMFEAVLVSFCDHGVTPPSTQSARLMASAGSSVNACIAGGILAFGKNHAGAIEISMKMLQRGIQLQKNYGKSTDEIKDEVLTYFENGNMKVPGFGHRYHKKDPRAAKLIEVAKKLDCFNEHCELAMNIENSLYNEKGIKMNIDGANSAILSDMGFDWKMGCGIFIIGRVPGLLAHVNEEMALEKPFRKVFDMNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I6</accession>
    <name>F0T9I6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644085.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0482"/>
    <dbReference type="EnsemblBacteria" id="ADZ08734">
        <property type="protein sequence ID" value="ADZ08734"/>
        <property type="gene ID" value="Metbo_0482"/>
    </dbReference>
    <dbReference type="GeneID" id="10276922"/>
    <dbReference type="KEGG" id="mel:Metbo_0482"/>
    <dbReference type="eggNOG" id="arCOG10261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782524_0_0_2"/>
    <dbReference type="OMA" id="HSHKAME"/>
    <dbReference type="OrthoDB" id="104860at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="17182" checksum="3229FF21B86B1568" modified="2011-05-03" version="1">MWFPSDEPKEILRGNNLDKLSIPSLGYLRILNENYEFILFLNDNLIVGAWCLDIKSLSELFQNEAMEVIKILPDSRIELFEINPRLFKTILDLNEECKLSLPIRIDMFWDKIGFNTNVSRETLLAKYRIKEPSEEHIKNLVSTYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J0</accession>
    <name>F0T9J0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644089.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0486"/>
    <dbReference type="EnsemblBacteria" id="ADZ08738">
        <property type="protein sequence ID" value="ADZ08738"/>
        <property type="gene ID" value="Metbo_0486"/>
    </dbReference>
    <dbReference type="GeneID" id="10276926"/>
    <dbReference type="KEGG" id="mel:Metbo_0486"/>
    <dbReference type="eggNOG" id="arCOG01502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="KACSKRC"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="18367" checksum="F0102C8A2BEF04A1" modified="2011-05-03" version="1">MDKILIQPDLCDGCKDCEQACGKLYGTPRISVREIEGSFYPIICQQCEDAPCKMICPTEAMGLEVLEAKCIGCGLCMMVCPFGSINIENRKAHKCNQCPDKDTPACIAACSKRAIAKVDTERMKLEKQKQHIEKLVGLGKKKKKSTDMISVLTANARTKKVLHKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N9</accession>
    <name>F0T9N9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645269.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09918">
        <property type="protein sequence ID" value="ADZ09918"/>
        <property type="gene ID" value="Metbo_1693"/>
    </dbReference>
    <dbReference type="GeneID" id="10278150"/>
    <dbReference type="KEGG" id="mel:Metbo_1693"/>
    <dbReference type="eggNOG" id="arCOG10439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125862_0_0_2"/>
    <dbReference type="OMA" id="REGKWLY"/>
    <dbReference type="OrthoDB" id="115633at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024265">
        <property type="entry name" value="DUF3788"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12663">
        <property type="entry name" value="DUF3788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="16084" checksum="086FD038020D6557" modified="2011-05-03" version="1">MSWGVFTDKEIKPSDKHVYQSLGGVKPLWDTIVSFVEEKYGVDGEFIFYGKNFGWALRYRKSGRVLIALYPGNEEYTVQIILNKTQIEEVLESNITEETRNMIERTTMIREGKWLYLKVDEERDLDEIKTLITARSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S6</accession>
    <name>F0T9S6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645306.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1731"/>
    <dbReference type="EnsemblBacteria" id="ADZ09955">
        <property type="protein sequence ID" value="ADZ09955"/>
        <property type="gene ID" value="Metbo_1731"/>
    </dbReference>
    <dbReference type="GeneID" id="10278188"/>
    <dbReference type="KEGG" id="mel:Metbo_1731"/>
    <dbReference type="eggNOG" id="arCOG02645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_9_2"/>
    <dbReference type="OMA" id="YCLIDFT"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="21851" checksum="94F3722744D2FB8D" modified="2011-05-03" version="1">MNEKKQLLLNAALKLFVEKGFHGTSTSEIAKTAGVATGTLFHHFKTKQELINSLYLYTKEQLLIAITIDYQDSEQFKSNLKRLWLNFTHFGIEKSDMYQFIIIFHSSPYITSLTKEQAETRFDDLLTFYRKGIENQEIKTVKDEMMVEFFLGTIFSTVNYFTKHPERLNEKNMNLSFELFWDGISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T1</accession>
    <name>F0T9T1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein TetR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1736"/>
    <dbReference type="EnsemblBacteria" id="ADZ09960">
        <property type="protein sequence ID" value="ADZ09960"/>
        <property type="gene ID" value="Metbo_1736"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1736"/>
    <dbReference type="eggNOG" id="arCOG02643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_3_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="188" mass="21886" checksum="E98718ED513F0261" modified="2011-05-03" version="1">MIFMTNKTEQKIMDAALEIFAKNGFKSSTTKAIAEKSGFTEMTLFRKFSTKKNLYEMVLNQNVKLMLEDFNKNVFNGKKYENVKDFIANFIIQTQQVMMNNFEVFYISLNEENKSMEKLMAEQTNAAGNYLKQHIKNPEIDYDTLGISIISFVYIVNLENYHNRKASFGKSAESVVENFIEMIYCMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W2</accession>
    <name>F0T9W2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA topoisomerase 1</fullName>
            <ecNumber evidence="11">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">topA</name>
        <name evidence="14" type="ordered locus">Metbo_0533</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644136.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0533"/>
    <dbReference type="EnsemblBacteria" id="ADZ08785">
        <property type="protein sequence ID" value="ADZ08785"/>
        <property type="gene ID" value="Metbo_0533"/>
    </dbReference>
    <dbReference type="GeneID" id="10276978"/>
    <dbReference type="KEGG" id="mel:Metbo_0533"/>
    <dbReference type="eggNOG" id="arCOG01305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_5_2_2"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="OrthoDB" id="14543at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PTHR11390"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="11">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="711"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="734"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="11">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ08785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="775" mass="86605" checksum="895ADAECDD27141D" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y3</accession>
    <name>F0T9Y3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="10" type="ordered locus">Metbo_0554</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644157.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0554"/>
    <dbReference type="EnsemblBacteria" id="ADZ08806">
        <property type="protein sequence ID" value="ADZ08806"/>
        <property type="gene ID" value="Metbo_0554"/>
    </dbReference>
    <dbReference type="GeneID" id="10276999"/>
    <dbReference type="KEGG" id="mel:Metbo_0554"/>
    <dbReference type="eggNOG" id="arCOG04459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="OrthoDB" id="18897at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="9">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="305" mass="34241" checksum="A7882D54DE2B0A49" modified="2011-05-03" version="1">MLNKLQKEIIELKKEKNAIILAHNYQTGDIQEIADFLGDSLELCIKASQIEDADMVVFCGVDFMAETAAILNPEKLIVLPDKNAECPMAHMLKAEEIKTYKKRYPDAAVVLYVNTLAEAKAEADILCTSANAVKVVESLDNDRILFGPDTNLGWFVSHQLKDKEIIPAPEGGHCYVHKMFNLADLYFLREKYPEADILVHPECDAEVQEYADYVLSTGGMMAHAKTSDKTTFIIATEYDLVTRLRRENPGKTFIPALDDALCETMKLHTLEKVKNSLLNQEFVVKVPEDIAKKAKTAIDRMLDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Y6</accession>
    <name>F0T9Y6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644160.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0557"/>
    <dbReference type="EnsemblBacteria" id="ADZ08809">
        <property type="protein sequence ID" value="ADZ08809"/>
        <property type="gene ID" value="Metbo_0557"/>
    </dbReference>
    <dbReference type="GeneID" id="10277002"/>
    <dbReference type="KEGG" id="mel:Metbo_0557"/>
    <dbReference type="eggNOG" id="arCOG00509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="OMA" id="AFGLHYC"/>
    <dbReference type="OrthoDB" id="72715at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="245"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="391" mass="44478" checksum="B18757CC0BE60855" modified="2011-05-03" version="1">MIKTIKENVYSVGVEDWNRRIFDELIQIPEGTSYNSYLVKGKDKIALIDTVDPAKTEELLENLRTLEVNIDYVIANHAEQDHSGSLPKILEHYPDAVVVTNTKCKELLTEFLLISEDKFMTVKDGDVLDLGEKTLKFIITPWVHWPDTMVSYLKQDKILFSCDFFGSHVATSHTFANDRDIYKPAKRYYAEIMMPFRTIIKNNLKKIGTEEIDIIAPSHGPIYKNSKFIMDAYADWTADDSKNEVIIPYISMHGSTERMVNYLVDVLIKKGVNVKPLNLTYTDMGEVALELVDATTMIIASPTVLTGPHPSVVYATYLANALRPKLKYVSVIGSYGWGGRMLEQIEGMIKNLKVETIEPLIIKGFPKEEDYKKLDEIAEQIFQKHSELGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z6</accession>
    <name>F0T9Z6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0568</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644170.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0568"/>
    <dbReference type="EnsemblBacteria" id="ADZ08819">
        <property type="protein sequence ID" value="ADZ08819"/>
        <property type="gene ID" value="Metbo_0568"/>
    </dbReference>
    <dbReference type="GeneID" id="10277013"/>
    <dbReference type="KEGG" id="mel:Metbo_0568"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_5_0_2"/>
    <dbReference type="OMA" id="FQGRGNM"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="347"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="517" mass="56413" checksum="251DAF702E1135C6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA43</accession>
    <name>F0TA43_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645344.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1770"/>
    <dbReference type="EnsemblBacteria" id="ADZ09993">
        <property type="protein sequence ID" value="ADZ09993"/>
        <property type="gene ID" value="Metbo_1770"/>
    </dbReference>
    <dbReference type="GeneID" id="10278227"/>
    <dbReference type="KEGG" id="mel:Metbo_1770"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="KVGGTYA"/>
    <dbReference type="OrthoDB" id="125987at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8098" checksum="B0EDA43D82CD500A" modified="2011-05-03" version="1">MFGNNYGNERENNAPIEEGSEYDVKIEDTGRDGDGIARVEGFVVFVSGAKVGDEVRIRVNSVRRNFAFADIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA67</accession>
    <name>F0TA67_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10017">
        <property type="protein sequence ID" value="ADZ10017"/>
        <property type="gene ID" value="Metbo_1795"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1795"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="PKYGFAS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="78" mass="8464" checksum="A5D9491285E0BEE5" modified="2011-05-03" version="1">MEVIILFGNSYGNDRGNDAPIKEGGEYDVKIEDTGRDGDGIARIEGFVVFVAGAKVGDEVKVRVNSVRRNFGFADIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA88</accession>
    <name>F0TA88_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oxidoreductase/nitrogenase component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1816</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1816"/>
    <dbReference type="EnsemblBacteria" id="ADZ10038">
        <property type="protein sequence ID" value="ADZ10038"/>
        <property type="gene ID" value="Metbo_1816"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1816"/>
    <dbReference type="eggNOG" id="arCOG00597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_2_0_2"/>
    <dbReference type="OMA" id="CIAIVHG"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014280">
        <property type="entry name" value="Nase_Fe-Fe_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02931">
        <property type="entry name" value="anfK_nitrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="3">
        <location>
            <begin position="16"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="458" mass="51210" checksum="381B93E273C2BEF6" modified="2011-05-03" version="1">MKKKERSGTINPIFTCQPAGAQYVSIGVENCIGIVHGGQGCVMFVRLLFAQHFKENFLLASSSLHEDTAVLGATHRIEEAVDVLLMRYPEVKVVPIISTCSTEIIGDDVEGVIRKLENGLLKEKYADREVHLISISTPSFKGSMVSGYDIAVRDFVRKFATKNEPNEKINLITGWVNPGDVTALKHLLKEMDVDANVLFETESFDSPLMPKGDAVSYGGTTIKDLESTGDAIGTIVLNRYEGKLAADFLETEFEIPSIIGPTPIGIRNTDAFLNNLKKMTGKPIPESLVRERGVAIDALTDLVHMFFADKKVAIFGNPDLVIGLAEFCIDLEMKPVLLLLGDDNVNYSRDPRIKEFEEKVEFEMEIIENADLWDLEKRIKNGDIELDLIIGHSKGRFIAIDNDIPMLRVGFPVYDRAGYYRHPIVGYAGATWLAEEMANVLFTDMEYKKNKEWILNVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA4</accession>
    <name>F0TAA4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="8">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">eif2g</name>
        <name evidence="10" type="ordered locus">Metbo_0605</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644207.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0605"/>
    <dbReference type="EnsemblBacteria" id="ADZ08856">
        <property type="protein sequence ID" value="ADZ08856"/>
        <property type="gene ID" value="Metbo_0605"/>
    </dbReference>
    <dbReference type="GeneID" id="10277050"/>
    <dbReference type="KEGG" id="mel:Metbo_0605"/>
    <dbReference type="eggNOG" id="arCOG01563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="OMA" id="NIGMVGH"/>
    <dbReference type="OrthoDB" id="25592at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="4"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="43838" checksum="918F7D3D80013446" modified="2011-05-03" version="1">MKIQSEINIGLVGHVDHGKTTLTKALSGIWTDTHSEETKRGISIRLGYADITFRRCTSCPAPQCFTTAEICSHCGEETSTLRKVSFVDSPGHETLMATMLSGAAIMDGAVLVIAANETCPQPQTKEHLMALDVIGVTDVIVVQNKIDIVSKERALESYKEIQEFVKGTCAENAPIIPVSAQQGANIDILIEVIQENIRTPRRSLRKSPRLFVARSFDTNKPGSSPDKIEGGVIGGSLIQGKLKVGDELEIKPGIQVKNKGKMEWMSLVSEITGLVGGGESVDEIGPGGLIGVGTKLDPALTKADSLSGSVAGKPGTLPPIMHDFTMRTHLLDRVVGTKEERKVEPIKSSELLMINIGTSTSVGVVTSARKNDVEVNLKLPVCAEEGQRVALSRRVGARWRLIGYGIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA6</accession>
    <name>F0TAA6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">infB</name>
        <name evidence="9" type="ordered locus">Metbo_0607</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644209.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0607"/>
    <dbReference type="EnsemblBacteria" id="ADZ08858">
        <property type="protein sequence ID" value="ADZ08858"/>
        <property type="gene ID" value="Metbo_0607"/>
    </dbReference>
    <dbReference type="GeneID" id="10277052"/>
    <dbReference type="KEGG" id="mel:Metbo_0607"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OMA" id="FRQSKPA"/>
    <dbReference type="OrthoDB" id="17053at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="3"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="595" mass="65735" checksum="8ACBC6A2C5D78875" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE5</accession>
    <name>F0TAE5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CO2 + diphosphate + nicotinate beta-D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644248.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0646"/>
    <dbReference type="EnsemblBacteria" id="ADZ08897">
        <property type="protein sequence ID" value="ADZ08897"/>
        <property type="gene ID" value="Metbo_0646"/>
    </dbReference>
    <dbReference type="GeneID" id="10277091"/>
    <dbReference type="KEGG" id="mel:Metbo_0646"/>
    <dbReference type="eggNOG" id="arCOG01482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="73201at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="8">
        <location>
            <begin position="16"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="7">
        <location>
            <begin position="105"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="30260" checksum="46D3148EF16E0C4A" modified="2011-05-03" version="1">MKYDLTEIIGMIHKDVGFEDITSTALIKPDTMIKARIISREIGIAAGIELACEIFTEFSVDANALVSDGEKIDASDVLMEIEGDARTILGVERTVLNLLMRMCGIATTTFNLVSKVHKIDPNMRVAATRKTTPGLQLFEKEAVKLGGGDSHRYRLDDCVLIKDNHIAIVGSVGDSVKIARSKVSFTKKIEIEVENMSDALEASIAGADIVMLDNMDPEEVKNVVKAIENQNLRDNILIEVSGGIGPNNIENYAGIGVDIISTGYLTHSVTALDLSLEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG3</accession>
    <name>F0TAG3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate dehydrogenase and fumarate reductase iron-sulfur protein</fullName>
            <ecNumber evidence="3">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.98.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644266.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0664"/>
    <dbReference type="EnsemblBacteria" id="ADZ08915">
        <property type="protein sequence ID" value="ADZ08915"/>
        <property type="gene ID" value="Metbo_0664"/>
    </dbReference>
    <dbReference type="GeneID" id="10277109"/>
    <dbReference type="KEGG" id="mel:Metbo_0664"/>
    <dbReference type="eggNOG" id="arCOG00333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00963">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_0_2"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="OrthoDB" id="21885at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="498" mass="55284" checksum="25CF930273EA414F" modified="2011-05-03" version="1">MIKVKILRQNPRTGKEPYFEVYDVEESDKMKILDALNQINNKYDADIAYRYSCRAGQCGSCALKMNNKIVLACKAEIKDGAVLEPLDFEVLKDLVVDRSKYDNKVTKMDLFVEGDCSVQECPQIMFPKDYADTKKIRSCIECLSCLSACPVVKQTEEFAGPYFMRYISKFALDPRDCADRAAEGFDEGLYTCTSCGKCKEVCPKEINTFGSAIEKLREISVQENIGPLPAHKTILEQIKKTGRSVEPAGTGEFPEGFVKTQKNVNNGSKHKIALFTGCMVDYKLPEIAVALLKVMEANNIDVIVPDGQICCGSPLLRTGQTEIFPEIAKRNYEALKDYDTIVTVCAGCGSTLKNDYPKAGINLNVKDISEFLADKLETDTMKPVNMKVCYHDPCHLVRGQGISKEPRELLNKIKGVEFVEMEVPDQCCGAGGGVRSGKPEIAAGLGSKKVKMVEKLDVDAVITICPFCEYNIQDSLDREGLNDVKAMNILKLLEMAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ0</accession>
    <name>F0TAJ0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Low temperature requirement A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645413.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10062">
        <property type="protein sequence ID" value="ADZ10062"/>
        <property type="gene ID" value="Metbo_1840"/>
    </dbReference>
    <dbReference type="GeneID" id="10278297"/>
    <dbReference type="KEGG" id="mel:Metbo_1840"/>
    <dbReference type="eggNOG" id="arCOG10808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045667_2_0_2"/>
    <dbReference type="OMA" id="HMAERCA"/>
    <dbReference type="OrthoDB" id="56029at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010640">
        <property type="entry name" value="Low_temperature_requirement_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36840">
        <property type="entry name" value="PTHR36840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06772">
        <property type="entry name" value="LtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="398" mass="44724" checksum="A8D1AA87C7E71AD7" modified="2011-05-03" version="1">MKLRKDFITVPILRYKGDRTDEKHADWLELLFDLIFVAAISRLALNLSDSYNLITFLESLPLFFAIWWGWAGHTFYLDRFGTDDIFSRIVTMLQMVAVASLAVNVKDALTTSGAGFAISYAMLRFILVAEYIWVGRKLPQASPLTNRYSLGFGLAATIWLISAFVPDPYRFVLWGVALLIDFLTPFTAGEIHINFPPHPTHLPERFGLFSIIVIGEAILSLEVAVSNIGTAFITELMGLMGLIIAFSLWWGYFEEAEGAEERVQDRGDQIAKYQLWLYSHFPFMLGVVGAAAGIKHVISLPFGFELASSDTWLMCLSLALALISISLIFISSFKWEDCRRRVLLNYRMPYYLVIALVIATGFLSTVVSGFTVLLILTILCIINVIISLRETPEAVCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ1</accession>
    <name>F0TAJ1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RQC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645414.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1841"/>
    <dbReference type="EnsemblBacteria" id="ADZ10063">
        <property type="protein sequence ID" value="ADZ10063"/>
        <property type="gene ID" value="Metbo_1841"/>
    </dbReference>
    <dbReference type="GeneID" id="10278298"/>
    <dbReference type="KEGG" id="mel:Metbo_1841"/>
    <dbReference type="eggNOG" id="arCOG10449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559500_0_0_2"/>
    <dbReference type="OrthoDB" id="127106at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RQC" evidence="1">
        <location>
            <begin position="65"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="19790" checksum="4C0D4578334721F1" modified="2011-05-03" version="1">MPFMICKNCDVYYEIADKNLVEDIKTCQCGTKMNYYEKLEDYLNLKLQKSVSEPSIEKLTSDYESALSRMILMSLKQVPVQLGIKRLMLVLKGSSSPFIFKYKINQLETYGILNNFSEEDLRYMVDVLIERGFIESEYLSQYEGSTLKCTVEGQEFLNGTETISLGFVKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK4</accession>
    <name>F0TAK4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1854</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645427.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10076">
        <property type="protein sequence ID" value="ADZ10076"/>
        <property type="gene ID" value="Metbo_1854"/>
    </dbReference>
    <dbReference type="GeneID" id="10278312"/>
    <dbReference type="KEGG" id="mel:Metbo_1854"/>
    <dbReference type="eggNOG" id="arCOG01964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_6_1_2"/>
    <dbReference type="OMA" id="THINFAM"/>
    <dbReference type="OrthoDB" id="119119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="10">
        <location>
            <begin position="136"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="30211" checksum="52A85C5B9C0C2892" modified="2011-05-03" version="1">MNININQRNIFQGFIIVLIIVDLVLLMFITFSTVSQPIFLTIVYFDLLVCFILFADFIYRIRQCDNKKAFIRKNWTDIIAMAPLDILYFSPITVPPNYMFIIRLFRFTRLIALFRREWKYISSFFKQTHINFAMGMLLFTMFAGTILFYLVEHSVNPEVHTFWDALWFTVTTMISGNSNIVPSTYYGEGISVLMMLIGISFVGILTASLASWLISKSKNNEEDSQEKKLENLQTDITEIKTDISNLTKDIKELKNSFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL2</accession>
    <name>F0TAL2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645435.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1863"/>
    <dbReference type="EnsemblBacteria" id="ADZ10084">
        <property type="protein sequence ID" value="ADZ10084"/>
        <property type="gene ID" value="Metbo_1863"/>
    </dbReference>
    <dbReference type="GeneID" id="40927933"/>
    <dbReference type="KEGG" id="mel:Metbo_1863"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_596679_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="458" mass="51156" checksum="F76C7A9FC2093566" modified="2011-05-03" version="1">MNNNKCGCEEDNLDKKTEEKIKEDNKCDCGCDNESPEDNHYAAETEDCGCGENSAKHEEDTGCGCGEEHDESHEEGGCCGDIEIPDLSRVENPVNPKFLAEPNFFKEIEEYAHSLGISNIGYTLLTSDLLIKDQFIQYPFTIVLTMEMDNEILETPPGDDAKDLNDTAYVRAFILTTKISDYIRKEGYGTEIADPMKSKVNLSLLGQKAGLGHIGDSGLLITPELGPRLKVSAIFVSIANLPIKENNEHAWIPEYCEICGKCVKACPENALVEVDSCCGSEVEIVKKNCIGCNQGCTYCIEACPFEEKGYEHVKNKFDKINAKLKEKQAKYFDVKLWNKWVIENTELFKDLVNDFTMAIAVADNENMLQIIRVDNDLKVDLKPIDELETCNADLLFVMDEKTVEELFKDFTTDKFAELLSSKEIDVYGLNDQLELKTKGYIAFLNKLGISQGDGSCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL5</accession>
    <name>F0TAL5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10087">
        <property type="protein sequence ID" value="ADZ10087"/>
        <property type="gene ID" value="Metbo_1866"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1866"/>
    <dbReference type="HOGENOM" id="CLU_3362565_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="35" mass="4087" checksum="5F0EAA09379CECE8" modified="2011-05-03" version="1">MAKTELYLFIIGFNKSLLRKINNDSMIIVTRENSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM7</accession>
    <name>F0TAM7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">4Fe-4S ferredoxin, iron-sulfur binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645450.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1878"/>
    <dbReference type="EnsemblBacteria" id="ADZ10099">
        <property type="protein sequence ID" value="ADZ10099"/>
        <property type="gene ID" value="Metbo_1878"/>
    </dbReference>
    <dbReference type="GeneID" id="10278336"/>
    <dbReference type="KEGG" id="mel:Metbo_1878"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727258_0_0_2"/>
    <dbReference type="OrthoDB" id="45763at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="16918" checksum="99471EE7376901F6" modified="2011-05-03" version="1">MLLEEKARQIIENECDDYFLGFGDLQNTDDPMIQGYNTLFNEYPTSISIGITLPFYNEYSTNPEIYNRTNCQLKTITMKICDLLEDEGYNSFSFPKTNDTNESYLSLHVIAANHANLGEIGENGLLTTPEVGSGVNWGTILTDAPLKISTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR0</accession>
    <name>F0TAR0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0685"/>
    <dbReference type="EnsemblBacteria" id="ADZ08936">
        <property type="protein sequence ID" value="ADZ08936"/>
        <property type="gene ID" value="Metbo_0685"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0685"/>
    <dbReference type="eggNOG" id="arCOG04901">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132457_1_0_2"/>
    <dbReference type="OMA" id="ETCFGAY"/>
    <dbReference type="OrthoDB" id="106827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012025">
        <property type="entry name" value="UCP005642_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09875">
        <property type="entry name" value="DUF2102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005642">
        <property type="entry name" value="UCP005642"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03272">
        <property type="entry name" value="methan_mark_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15927" checksum="9E6C9A0AD9C8E47C" modified="2011-05-03" version="1">MLQDESSKITRMIILGPSANISQTELVRKLHMMELPITIKSTCYGAVVNGEESAVDKAVEKARNLDYCNIFTKDRGFPPGDPRRCRAKRGGAREGFHQLEKEFELLGFVSEALEHPHKVSLKKPKKVPVDTFKEVVKECEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT9</accession>
    <name>F0TAT9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644316.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0714"/>
    <dbReference type="EnsemblBacteria" id="ADZ08965">
        <property type="protein sequence ID" value="ADZ08965"/>
        <property type="gene ID" value="Metbo_0714"/>
    </dbReference>
    <dbReference type="GeneID" id="10277161"/>
    <dbReference type="KEGG" id="mel:Metbo_0714"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_2"/>
    <dbReference type="OMA" id="HKAFGIH"/>
    <dbReference type="OrthoDB" id="15038at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="470" mass="50572" checksum="886C23630FD1612E" modified="2011-05-03" version="1">MSGLKRSLGLYDVISLVIGTIVGADIYIAASFGAGLLGPFSVVAWVIAGLMAVIIALCFAECSSRVPQVGGPYAYAKRAFGEFTGFLVGWALLIASWSAIAVFPLAFVAYLAFFIPNMSPELVIVIKILFVLFLTVVNYFGVREAGKLNDVLTILKIAPIIILTIAGIIFFVLKPSLLVSNFTPFIPLGLNGLGSAIVLIFWAYVGFELVTVPSDEIVNPQRTIPMAIAIGMAVITVFYVLTNFVILGLVPWAALASSTAPLALAGYAILGAIGAGFLTLGALLSISGSDEAGILSAARIPYAMAADGYLPRALAKVHPKYETPYVALIIQSTVTLIAAIFGTISQLIVLSVFTLLFCYLLTCLSVFPLRKKFKEGIKLPWIVPVLGVIISIYIMTQCELNQILIGTALILVGIPVYVLFAPKTEITTVKRDLKEGEDYVSRTLEADEIFLAKFLNQVKELVTRIKDRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU9</accession>
    <name>F0TAU9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0724</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644326.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0724"/>
    <dbReference type="EnsemblBacteria" id="ADZ08975">
        <property type="protein sequence ID" value="ADZ08975"/>
        <property type="gene ID" value="Metbo_0724"/>
    </dbReference>
    <dbReference type="GeneID" id="10277171"/>
    <dbReference type="KEGG" id="mel:Metbo_0724"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_4_0_2"/>
    <dbReference type="OMA" id="PKVKYLN"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="PTHR43078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="7">
        <location>
            <begin position="5"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="35625" checksum="42B421A14F0B658D" modified="2011-05-03" version="1">MVTVIITGVAGFVGSHLADKFVNKGFHVIGIDNFLTGSIENISSLLDKDNFEFIEHDIIKPIKLDEKIDIILHFACPASPEDYLQYPLETLRVDSIGTYNTLEMSRENNARYIFASTSEIYGDPLVNPQPETYWGNVHSTGLRSVYDESKRFSEALSMAYYREYCLDVRIVRIFNTYGPRMKLNDGRVVPNFISQALKNEDLTVYGEGTQTRSFCYVKDLVEGIFKISNTDNLNGNIMNLGNPDEYKILDFAKIIINKTNSNSKITFKDLPEDDPKLRCPDISKVQSLIEWKPQTSLDNGLQETIKFLKSKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV3</accession>
    <name>F0TAV3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transferase hexapeptide repeat containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644330.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0728"/>
    <dbReference type="EnsemblBacteria" id="ADZ08979">
        <property type="protein sequence ID" value="ADZ08979"/>
        <property type="gene ID" value="Metbo_0728"/>
    </dbReference>
    <dbReference type="GeneID" id="10277175"/>
    <dbReference type="KEGG" id="mel:Metbo_0728"/>
    <dbReference type="eggNOG" id="arCOG01848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_9_0_2"/>
    <dbReference type="OMA" id="QIGWMSE"/>
    <dbReference type="OrthoDB" id="93082at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="22214" checksum="083C242C111F665D" modified="2011-05-03" version="1">MVKFRNLLLNHQDEIVEKGNNNKNIILGVEYKSKSELPVIGKNSFIRSNTVIYNDVNIGDNFTTGHGVVVREKTYIGDNVLIGTNSIVEGYTSIGNDVNIQSSVYIPKNSLIEDNVFIGPCACFTNDKYPLRIDYDLKGPVIRTGASIGSNSTFLSDIEIGKGAMVAAGAIVTIDVPEYFLAIGAPARIKPLPKHLKKLNKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY9</accession>
    <name>F0TAY9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hel308</name>
        <name evidence="4" type="ordered locus">Metbo_1915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1915"/>
    <dbReference type="EnsemblBacteria" id="ADZ10135">
        <property type="protein sequence ID" value="ADZ10135"/>
        <property type="gene ID" value="Metbo_1915"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1915"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_0_2"/>
    <dbReference type="OMA" id="DYSFEDH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="32"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="208"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="690" mass="77647" checksum="4F46520D86AA63EE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB11</accession>
    <name>F0TB11_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645508.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1937"/>
    <dbReference type="EnsemblBacteria" id="ADZ10157">
        <property type="protein sequence ID" value="ADZ10157"/>
        <property type="gene ID" value="Metbo_1937"/>
    </dbReference>
    <dbReference type="GeneID" id="10278397"/>
    <dbReference type="KEGG" id="mel:Metbo_1937"/>
    <dbReference type="eggNOG" id="arCOG03177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_23_0_2"/>
    <dbReference type="OMA" id="PMMTSQV"/>
    <dbReference type="OrthoDB" id="104820at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="19112" checksum="5D6287230E0F96C4" modified="2011-05-03" version="1">MIDNDKYVKYWREEPEESVGFLFWQITHLWQRKMNLALKELDLTHVQFALLSGIAWLERFDEDITQVKLANHAKTNIMMTSKVLKTLEKKNLISREECEFDTRAKCLSITDDGRERIEKALQIVEEMENKFFGGQTNDQDFINNLFNILQLNQQEYFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB13</accession>
    <name>F0TB13_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645510.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1939"/>
    <dbReference type="EnsemblBacteria" id="ADZ10159">
        <property type="protein sequence ID" value="ADZ10159"/>
        <property type="gene ID" value="Metbo_1939"/>
    </dbReference>
    <dbReference type="GeneID" id="10278399"/>
    <dbReference type="KEGG" id="mel:Metbo_1939"/>
    <dbReference type="eggNOG" id="arCOG06113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2875115_0_0_2"/>
    <dbReference type="OMA" id="NAPLWEC"/>
    <dbReference type="OrthoDB" id="124375at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="7142" checksum="9F26F3D7DCA70791" modified="2011-05-03" version="1">MSTSSCPRCGSKMMKIHNPNAPLWECSKCGYTGSVVIKDGNIEKQLRETRKLEKLSKKMLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB29</accession>
    <name>F0TB29_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1955"/>
    <dbReference type="EnsemblBacteria" id="ADZ10175">
        <property type="protein sequence ID" value="ADZ10175"/>
        <property type="gene ID" value="Metbo_1955"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1955"/>
    <dbReference type="eggNOG" id="arCOG02823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_574423_0_0_2"/>
    <dbReference type="OMA" id="FEYGIRD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="74"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="491" mass="54776" checksum="7FF4C84533BF479B" modified="2011-05-03" version="1">MEEFKYLVGIVRCMGLSGKIRFKTAKVSGKLAKSLVKVGKGMGKSFPGYLFLKIGSYDCLKELAKTPRVGSIIVTGTNGKTTTTKLISLLLENDAPISYNYDSNTLNAITTGLLSNNIDYGVFEYGIRDVAHAFPDEVCKLVAPIGVVYTNVSREHSLVAGVSNPFDKYLMAKELLCTPMKTGVVICNADDPRTASIGTKKEAEIRVNYYGFDLESEDKTMAKPDVDCPLCGSKLEYSTMFSNHRGKYSCQCGFERPEPNMKLTELTKEFDAWYVTLEGEVYNHTTEKEIPVFLKIKTPAFGLYNLYNLLCAVTTYASFTNKPENIESTVKKITNSLDLSILPPGRFEIMKVGDKLVGMGQGDNGDALNANVQFMCEYVKDDLGFIYTTPDTGEDEIFEDHLQSVIAANPKKVYVVPGRTSVDAAKNYYKIIAENLDADFYPLPYKEMEERRSKIIDLILNSPYKYVLVSGCGPEHYMWAELKSELKTHMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB42</accession>
    <name>F0TB42_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645539.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1968"/>
    <dbReference type="EnsemblBacteria" id="ADZ10188">
        <property type="protein sequence ID" value="ADZ10188"/>
        <property type="gene ID" value="Metbo_1968"/>
    </dbReference>
    <dbReference type="GeneID" id="10278428"/>
    <dbReference type="KEGG" id="mel:Metbo_1968"/>
    <dbReference type="HOGENOM" id="CLU_736958_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="375" mass="40999" checksum="1D2BB6F4D0873266" modified="2011-05-03" version="1">MFGVVASAFSGFATIYAASNNHKDITTGMHIDINDHGPNQIIDITSYEGDSSKIKAEVWWNCYGGTTDPFTNVYMTIYDKTGRVVCTEVKESNIIGNCYFDVPDNLASGNYEIKFSASGDSSTGQNIYRAETWANLVVCDPVRGNPNAANGNLNVNAGNIIQGQKLSASANFVNSTRMANYKNQTPDKNSDLGYIDYVVVDDKNNIVMRGCGTLKLSNNQIISDSDLNTANLQPGTYHLQSQIQLNSFIKPINTEQTFNVYSKNSSLSVSNESTLNVTKGNDAQVVAWINNDGLANISNKTAPKASVTFTVFDQNYNVITGDTFPATLVNGHYVAYMDWNTANFKPGTYKVHMAVNGNPYLSDCDSWTTITINPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB47</accession>
    <name>F0TB47_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645544.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10193">
        <property type="protein sequence ID" value="ADZ10193"/>
        <property type="gene ID" value="Metbo_1973"/>
    </dbReference>
    <dbReference type="GeneID" id="10278433"/>
    <dbReference type="KEGG" id="mel:Metbo_1973"/>
    <dbReference type="eggNOG" id="arCOG14013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519778_0_0_2"/>
    <dbReference type="OMA" id="YTLINGH"/>
    <dbReference type="OrthoDB" id="139270at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9395" checksum="6A982CB9519691C4" modified="2011-05-03" version="1" precursor="true">MAEIKKNKLKAIIGALITFIVVGLLAVQAMFFLKGYAPSTNMMNHQFLVSIGFLIGILAASYVYTYLQGEDRSSWLYDKDGKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB48</accession>
    <name>F0TB48_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">OMP decarboxylase</fullName>
            <shortName evidence="5">OMPDCase</shortName>
            <shortName evidence="5">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrF</name>
        <name evidence="8" type="ordered locus">Metbo_1974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645545.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1974"/>
    <dbReference type="EnsemblBacteria" id="ADZ10194">
        <property type="protein sequence ID" value="ADZ10194"/>
        <property type="gene ID" value="Metbo_1974"/>
    </dbReference>
    <dbReference type="GeneID" id="10278434"/>
    <dbReference type="KEGG" id="mel:Metbo_1974"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="OMA" id="EMSHPGA"/>
    <dbReference type="OrthoDB" id="54384at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23232" checksum="99827473D00B444E" modified="2011-05-03" version="1">MEIKNKIILAMDLLTIDGALEVGNSVINYLDTIKIGYPLALAEGLPSIGLVKDRFDCKIIADFKVADIPETNKKIADLTFKAGADAIIVHGFVGPDSVKACVDSAEEFGTEVFLLTEMSHPGASRFLQPAAEDIAVMGMEMGLKNYVAPSTKPDRLKKIREVVGKDSFIISPGVGVQGGDPNQTLEFADAIIVGRSIYSSEYPEEVVKSIVDSIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB69</accession>
    <name>F0TB69_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644371.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0769"/>
    <dbReference type="EnsemblBacteria" id="ADZ09020">
        <property type="protein sequence ID" value="ADZ09020"/>
        <property type="gene ID" value="Metbo_0769"/>
    </dbReference>
    <dbReference type="GeneID" id="10277217"/>
    <dbReference type="KEGG" id="mel:Metbo_0769"/>
    <dbReference type="HOGENOM" id="CLU_2021522_0_0_2"/>
    <dbReference type="OrthoDB" id="137735at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="14403" checksum="992E15C9DBBE698D" modified="2011-05-03" version="1">MEEEHPMGELIIPVEGLLKEELQEEIEERIAFTVENLYHLDNELDVFVQLVRENVESNKNSPDDLMTLYNYLDEMWSVLITNDLRDVLLDTLSIVQARKHELEDILEEIEETLEKQEEESSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE2</accession>
    <name>F0TBE2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">albA</name>
        <name evidence="9" type="ordered locus">Metbo_1991</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645562.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1991"/>
    <dbReference type="EnsemblBacteria" id="ADZ10211">
        <property type="protein sequence ID" value="ADZ10211"/>
        <property type="gene ID" value="Metbo_1991"/>
    </dbReference>
    <dbReference type="GeneID" id="10278451"/>
    <dbReference type="KEGG" id="mel:Metbo_1991"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OMA" id="KPTTNYI"/>
    <dbReference type="OrthoDB" id="111461at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="SSF82704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00285">
        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Alba" evidence="8">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10332" checksum="D85C3EB09DFF823B" modified="2011-05-03" version="1">MSEENVVYIGNKPVMNYVLAVVTQMNSGVPEVMLKARGRAISRAVDVAEIVRNRFLPELEVGSIDICTEEMTSREGSNSNVSAIEIQLCKTESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG6</accession>
    <name>F0TBG6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645586.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10235">
        <property type="protein sequence ID" value="ADZ10235"/>
        <property type="gene ID" value="Metbo_2016"/>
    </dbReference>
    <dbReference type="GeneID" id="10278476"/>
    <dbReference type="KEGG" id="mel:Metbo_2016"/>
    <dbReference type="HOGENOM" id="CLU_2340202_0_0_2"/>
    <dbReference type="OrthoDB" id="128215at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="97" mass="11246" checksum="C4329E59F4830056" modified="2011-05-03" version="1">MVEVQILEFGYSLEKGRHFIRFLVTGLKDKDIEKIGGIVGQIPEGSFKRFQTWNTDTGLEIYELFPAKEYPFNKEIPSPEEFKAVEKNVQGFLARFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI6</accession>
    <name>F0TBI6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acetolactate synthase</fullName>
            <ecNumber evidence="9">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2036</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645606.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2036"/>
    <dbReference type="EnsemblBacteria" id="ADZ10255">
        <property type="protein sequence ID" value="ADZ10255"/>
        <property type="gene ID" value="Metbo_2036"/>
    </dbReference>
    <dbReference type="GeneID" id="10278497"/>
    <dbReference type="KEGG" id="mel:Metbo_2036"/>
    <dbReference type="eggNOG" id="arCOG01999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_0_2"/>
    <dbReference type="OMA" id="YEATHEC"/>
    <dbReference type="OrthoDB" id="7134at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="585" mass="65123" checksum="9FFE8CAE02221E88" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ7</accession>
    <name>F0TBJ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase of anthranilate synthase</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645617.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2047"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="EnsemblBacteria" id="ADZ10266">
        <property type="protein sequence ID" value="ADZ10266"/>
        <property type="gene ID" value="Metbo_2047"/>
    </dbReference>
    <dbReference type="GeneID" id="10278509"/>
    <dbReference type="KEGG" id="mel:Metbo_2047"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OMA" id="TEHGHAM"/>
    <dbReference type="OrthoDB" id="100703at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21045" checksum="A91DEC406BBE5C89" modified="2011-05-03" version="1">MILIIDNYDSFTYNLYQLIGQLDPDIKVVKNDELNLDEIRKLEPDSIVISPGPGNPGNKRDFGVCNDVILELGGEIPILGVCLGHQGIFAAFGGEIVRNQPVHGKQSSIVHDGKGLFKGLENPLPAARYHSLSCSEKTTPECVEVTARTKDDMIMGIKHVEKPIYGLQFHPESVGTDHGIQILKNFLEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM6</accession>
    <name>F0TBM6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644454.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0853"/>
    <dbReference type="EnsemblBacteria" id="ADZ09103">
        <property type="protein sequence ID" value="ADZ09103"/>
        <property type="gene ID" value="Metbo_0853"/>
    </dbReference>
    <dbReference type="GeneID" id="10277302"/>
    <dbReference type="KEGG" id="mel:Metbo_0853"/>
    <dbReference type="eggNOG" id="arCOG10138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036186_0_0_2"/>
    <dbReference type="OMA" id="WKDHHAS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039498">
        <property type="entry name" value="NTP_transf_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14907">
        <property type="entry name" value="NTP_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="383" mass="44652" checksum="1380A8BCD5FAE4D2" modified="2011-05-03" version="1">MNNDKDFNLNTEDKILLFCARNRSDPENKLELEAMIHEDLNWQYLFEIATIHKLKPLLYYNLNTIVPNRTSKNFLDDLKIYYKKNARKNLLLTGELIRVTNSLNSQNINSFTYKGPTLATLAYKNIALRDFSDIDLVVSLKNVKKSIIVLKNMGYTSTFPSMLEKGNICLKYHRAHVLSKGNVNIDLQWGLSFIKHENLNNFETKDLIKININKIELLTPNVENQIIILVMHNASHMWHSLSLLCDLDGLISMNKVNWNEVKSLATKIGINRILLINLLLLKNVMGNPVLTEYWGDTLDIKAEKLASKIEKNFFSPEKFADSFFKRALLQFQIRENKIDGLSDLFYLVFNPTAEDYQRIYIPNSIFKLYYLIRLFNLIKKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP1</accession>
    <name>F0TBP1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644469.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0869"/>
    <dbReference type="EnsemblBacteria" id="ADZ09118">
        <property type="protein sequence ID" value="ADZ09118"/>
        <property type="gene ID" value="Metbo_0869"/>
    </dbReference>
    <dbReference type="GeneID" id="10277318"/>
    <dbReference type="KEGG" id="mel:Metbo_0869"/>
    <dbReference type="eggNOG" id="arCOG07652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352117_0_0_2"/>
    <dbReference type="OMA" id="IVFRTIN"/>
    <dbReference type="OrthoDB" id="115637at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="24212" checksum="6919433E794018E3" modified="2011-05-03" version="1">MEDSEILDKIDKNTNIIFYDKNVYKCYTKLKEDHICVYFNEPTPIKGRLIEIINTIGVNDKNPLNPMTIPDLTDLIINDPEYDRLIIIFNNFERLTKRTAQVYQQLYNSPNIQFICSFSEQFKKEIYPFFKQFEFVNNEEYEKNSTSNEINVTYVFYIIITFYCSLFYLKFVSSALQNVYVLIGAIWFVLIIFRTLFYMGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY9</accession>
    <name>F0TBY9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate dehydrogenase and fumarate reductase iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645682.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2112"/>
    <dbReference type="EnsemblBacteria" id="ADZ10331">
        <property type="protein sequence ID" value="ADZ10331"/>
        <property type="gene ID" value="Metbo_2112"/>
    </dbReference>
    <dbReference type="GeneID" id="10278575"/>
    <dbReference type="KEGG" id="mel:Metbo_2112"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_1_2"/>
    <dbReference type="OMA" id="CTHCYRC"/>
    <dbReference type="OrthoDB" id="88832at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="29295" checksum="D6512718851438B3" modified="2011-05-03" version="1">MKFKIFRFKEGMENPSYDKFEFEPPTGMTVLGALFYIQQNFDDSIAFHYSCRGAVCGTCGMLINKVPRLACRTQLEPLIHGELKVPLSPYSAIEETGAWDPEEEILIEPLPHLSVKKDLIVDMDRFFDYYQQVEPVLQPADPEPTLERLMDPEAVKELELYTNCILCALCFGSCPVDGAKPDYLGPAALAKLYRFYIDPREGKNESRLLLADKPNGWWDCEFHGNCRRVCPKDVPPLIAIAKAREKLNIIKNRKDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC13</accession>
    <name>F0TC13_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644515.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09164">
        <property type="protein sequence ID" value="ADZ09164"/>
        <property type="gene ID" value="Metbo_0915"/>
    </dbReference>
    <dbReference type="GeneID" id="10277364"/>
    <dbReference type="KEGG" id="mel:Metbo_0915"/>
    <dbReference type="eggNOG" id="arCOG02449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068049_0_0_2"/>
    <dbReference type="OMA" id="IEHPAMK"/>
    <dbReference type="OrthoDB" id="73968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026816">
        <property type="entry name" value="Flavodoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12724">
        <property type="entry name" value="Flavodoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="29406" checksum="BFCEF5B93815BD66" modified="2011-05-03" version="1">MIFYFSGTGNSLYVAQKLQETEGGELINITDALNKKQFKYQTVDGEKVGIIFPVYFNGLPTIVEQFMEKLTIDNSNQPFIYTVVTCGSSIGRADKMVANQLQTKDLELSSAFSVEMPSNYVMIYDTYDAEKEKSKLQVAEKQIENIKKLLKTNKKGNFSKHGIIAILTPLIYKLYGMRRNTEKFYTTDACNGCALCEEICPEMAIKLENGKPEWTKDKCSHCTACINRCPKRAIQYGKATEKRGRYINPNVVFEVNKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC53</accession>
    <name>F0TC53_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="4">AGPR</shortName>
            <ecNumber evidence="4">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="4">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">argC</name>
        <name evidence="7" type="ordered locus">Metbo_0957</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644555.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0957"/>
    <dbReference type="EnsemblBacteria" id="ADZ09204">
        <property type="protein sequence ID" value="ADZ09204"/>
        <property type="gene ID" value="Metbo_0957"/>
    </dbReference>
    <dbReference type="GeneID" id="10277406"/>
    <dbReference type="KEGG" id="mel:Metbo_0957"/>
    <dbReference type="eggNOG" id="arCOG00495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="OrthoDB" id="51061at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="6">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="336" mass="37022" checksum="7A70093BF746B5C0" modified="2011-05-03" version="1">MIEVGIIGASGYTGGELIRFLMKHPDVDIVAATSRKFDDEPVYKVHPNLRDCELKFKNIEPSKLEADLVFTATPHGASMKIVPELIDKNIRVVDLSGDYRFESTEIYEKWYGFKHSNPLNAVYGLPEINRKEIKHADLVANPGCYPTGAILACLPIVSENLADNIVVDSKSGVSGAGVNPTDTTHYPNCSDSVSPYAVTTHRHGPEIQEKLSKYGNSKVSFTPHLVPVIRGIMTTVHGFLTSDVSPEYIHELYKEFYDGEPFVRVLEKGESPRMSSVRGSNNCHIGCFEIDENGRLVVASSIDNLVKGASGQAVHNMNLMFGFKETESIDNIGLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC66</accession>
    <name>F0TC66_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644568.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0970"/>
    <dbReference type="EnsemblBacteria" id="ADZ09217">
        <property type="protein sequence ID" value="ADZ09217"/>
        <property type="gene ID" value="Metbo_0970"/>
    </dbReference>
    <dbReference type="GeneID" id="10277419"/>
    <dbReference type="KEGG" id="mel:Metbo_0970"/>
    <dbReference type="eggNOG" id="arCOG05092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134280_4_0_2"/>
    <dbReference type="OMA" id="IAINERY"/>
    <dbReference type="OrthoDB" id="129748at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019277">
        <property type="entry name" value="DUF2304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10066">
        <property type="entry name" value="DUF2304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="126" mass="14354" checksum="41F73FC685E9DA7B" modified="2011-05-03" version="1">MLMIYQVIAIILGILAIVLSILRFRDGKMSLGMMFVWILIWLGVIFIAIDPNSTNFLAVYTGIGRGLDFVLIIGLLLCFYLIFKMYNKIETVEEELTDLVRELALARKMGEDTKKQDKTKSSSDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE8</accession>
    <name>F0TCE8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase accessory protein HypB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645766.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2201"/>
    <dbReference type="EnsemblBacteria" id="ADZ10415">
        <property type="protein sequence ID" value="ADZ10415"/>
        <property type="gene ID" value="Metbo_2201"/>
    </dbReference>
    <dbReference type="GeneID" id="10278665"/>
    <dbReference type="KEGG" id="mel:Metbo_2201"/>
    <dbReference type="eggNOG" id="arCOG01231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056148_0_1_2"/>
    <dbReference type="OMA" id="ECHLDAH"/>
    <dbReference type="OrthoDB" id="76245at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00073">
        <property type="entry name" value="hypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="38"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="217" mass="23554" checksum="90346096F4DEDD1F" modified="2011-05-03" version="1">MHKIADIEIQHDIMVANSKLAGKNQKILDDADVFSVDVLGAIGSGKTSLIETLIQKMDHNIGVIAGDVISKYDAGRFEQYDIPVVGLNTGKECHLDAHLVKHAFGDLPLESIDLLFIENVGNLICPVDFDLGSHIRMVVISVSEGDDTVEKHPLIFQNADLVVINKVDIADAVGADQDKMVADVKILNPDVKVIKSSLKTGQGLDEIISSIESFMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI0</accession>
    <name>F0TCI0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1010</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644608.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1010"/>
    <dbReference type="EnsemblBacteria" id="ADZ09257">
        <property type="protein sequence ID" value="ADZ09257"/>
        <property type="gene ID" value="Metbo_1010"/>
    </dbReference>
    <dbReference type="GeneID" id="10277459"/>
    <dbReference type="KEGG" id="mel:Metbo_1010"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048120_0_0_2"/>
    <dbReference type="OMA" id="ENCIDNE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="265" mass="29930" checksum="C00578DEE0DA272F" modified="2011-05-03" version="1">MVSNEEIKRRLERKRKGLSEIQQDEKTGKYKTCPHCKFKNPEKSIFCVKCGKKLETNVSITCSTCGTINPKTAKFCIKCGNNLTTQESTKTKEEEKPQNLVEDEKTEDENCIDNEPSVESELEVENIQSDIQNQETVPDADTKSSEVDEKEKSEINHIKTQGLPSSIPDQSLINQRDSKKTCKSCGAKNLKTAKFCVVCGEKFDIPPVEKSVEYPKAHENNKEDLKSAEIDDPMDKIKKAKELLDIGAITPEEFESIKSKYLDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL3</accession>
    <name>F0TCL3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09290">
        <property type="protein sequence ID" value="ADZ09290"/>
        <property type="gene ID" value="Metbo_1043"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1043"/>
    <dbReference type="eggNOG" id="arCOG10373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170804_0_0_2"/>
    <dbReference type="OMA" id="TDIMEEK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="10379" checksum="0C4AC30CA4008B7C" modified="2011-05-03" version="1">MMSEKFYKKLMEITKVMEEKGEGISYIHNEAENYTIGTKKYNEEIAKYLDQTVGTWEGSEAHVKKKKGKNKQEKGKKVGYLWKIPITES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM0</accession>
    <name>F0TCM0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644648.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1050"/>
    <dbReference type="EnsemblBacteria" id="ADZ09297">
        <property type="protein sequence ID" value="ADZ09297"/>
        <property type="gene ID" value="Metbo_1050"/>
    </dbReference>
    <dbReference type="GeneID" id="10277499"/>
    <dbReference type="KEGG" id="mel:Metbo_1050"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_1_6_2"/>
    <dbReference type="OMA" id="EFSQNDI"/>
    <dbReference type="OrthoDB" id="116946at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="114" mass="13020" checksum="EFCF9D8E541469B4" modified="2011-05-03" version="1">MLKFVTINPQDAFKIMEEKGSEITILDIRPKNEYEEEHVPGAVNLDYNGHEFKKKVEIMDKNVDYIIYCESGVRGGYFMGKMEESGFKSAYNILGGFVGWKISELPTVSNNKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN4</accession>
    <name>F0TCN4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09311">
        <property type="protein sequence ID" value="ADZ09311"/>
        <property type="gene ID" value="Metbo_1065"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1065"/>
    <dbReference type="eggNOG" id="arCOG06201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038031_0_0_2"/>
    <dbReference type="OMA" id="RIVIFGH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="PTHR34990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="27"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="422" mass="47886" checksum="468C2BAEA8D61F70" modified="2011-05-03" version="1">MTSKNSVESDINEDVKSIEVVEGERSWIVCISDLHLGADDTYSELTKNRDALIDFLNWVRFSPNIKELVIAGDLIDEWFVPMNLDTFKGKTQMDFVKNVALNNKPVIDAFNDIINDGQIRLTYVPGNHDILITAEEIEAILPGINQSRDVRGLGAYSPDYLPELVIEHGHRYNFFCAPDHSNRTVTQTDSILPTGYFFTRMATSSVVQGRPKIDMNIPIVTENELGEEQYLYYLYYKAWEKLINVFPVNEGINEPAIHTGIDGFTNSYAIKDVLPYQDPENNYIDVNLYKGIVESWEERQANNLVPILIPTEEAILKAALSSHLDDQSANQFFVNPHSNKRIVIFGHTHDARLITSYNENMDANVYVNSGTWIDSKDLTMTFVVVIPPKDDKSNKGYVGLYQYADEGKISKLDSQIINNLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN9</accession>
    <name>F0TCN9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645780.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2215"/>
    <dbReference type="EnsemblBacteria" id="ADZ10429">
        <property type="protein sequence ID" value="ADZ10429"/>
        <property type="gene ID" value="Metbo_2215"/>
    </dbReference>
    <dbReference type="GeneID" id="10278681"/>
    <dbReference type="KEGG" id="mel:Metbo_2215"/>
    <dbReference type="eggNOG" id="arCOG02820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022291_4_2_2"/>
    <dbReference type="OMA" id="IVIRHWI"/>
    <dbReference type="OrthoDB" id="27985at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="111"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="1">
        <location>
            <begin position="337"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="486" mass="53572" checksum="889DFD9D55145AC2" modified="2011-05-03" version="1">MTELTTSYLANKIDGKLTGPDIVIQGIFTFLNHAKPGDAVIRHWIDEKGVEIARNRGVSCVVTQNPKGDAVKTAQELDFPLIITPKIELANAFALRWSIEKFAPGSIRVVVTGTNGKSTTTHMIQNILNHAGYNCYTNTDSKSEFNTLIDPVVSDQISRFKAKTKKPIDAMVVEVSEVQGWMDKLMKDHAYLMTDAVKPDVLVLTNITMDHIGLINSIEETYAEIYGALAAVEKSCQNNYAVLNYDDPLLRKMAGEIVTCPSIISHGSVEPDFNPDLTVKSDGIHHEDELFLKMEDLPFKSKHFLQNTMAAIGACLALNIDTSAIKAGILSYSPLNRRFSILYQEPLIIDDFAHNPDGITATIKSASEMSEGTLIILFAVRGSRGEVINRLNAEALASALQAVDYSLIITSSSEVVDELNTVHASEIEVVRKVMDEHKLEHTFKEELEDALNYTLNTAKKEDTILLIGAQGMDPAKDILNRIKGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV2</accession>
    <name>F0TCV2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645843.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2278"/>
    <dbReference type="EnsemblBacteria" id="ADZ10492">
        <property type="protein sequence ID" value="ADZ10492"/>
        <property type="gene ID" value="Metbo_2278"/>
    </dbReference>
    <dbReference type="GeneID" id="10278744"/>
    <dbReference type="KEGG" id="mel:Metbo_2278"/>
    <dbReference type="eggNOG" id="arCOG04860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="OMA" id="WALWNAY"/>
    <dbReference type="OrthoDB" id="25508at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_beta_N" evidence="9">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_beta" evidence="8">
        <location>
            <begin position="189"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="443" mass="47106" checksum="A1B5948D0E878C9C" modified="2011-05-03" version="1">MPTYEDKIDLYGVNGKLLESDVPLEAVSPMVNPTIGKIVYDVKRSVAVNLAGIEKSLEKAAYGGKSNFIPGRELKLDLKGNVDVLSEKIEKIIKIDEDDDFNLKLINGGDQMLVQMPAQRINRAADYTVSTLVAGGAVVQAIIDTFDVDKFDASAVKTAVLGRYPQSVDFMGANVSALLGPPVMLEGLGYGLRNIMANHVVAITKKNTLNAVALSSILEHTAMFETGDALGAFERSHLLGMAYQGLNANNLVFDLVKENGKGTVGTVIATLVERAIDDGVIKVAKTMPSGYKVYEPNDWALWNAYAAAGLIASVIVNIGASRAAQGIASTLLYFNDIVEYETGLPGVDFGRAQGTGVGMSFFSHGIYGGGGPGTFHGNHVVTRHSKGYVTPCNAAAMCLDAGTQMFSVESTSGLIGTVYGDIDYFNQPVKYVADGAKEVKDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X0</accession>
    <name>F0T5X0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RelA/SpoT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644714.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1118"/>
    <dbReference type="EnsemblBacteria" id="ADZ09363">
        <property type="protein sequence ID" value="ADZ09363"/>
        <property type="gene ID" value="Metbo_1118"/>
    </dbReference>
    <dbReference type="GeneID" id="24964533"/>
    <dbReference type="KEGG" id="mel:Metbo_1118"/>
    <dbReference type="eggNOG" id="arCOG10830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058756_1_0_2"/>
    <dbReference type="OMA" id="QSVHYVV"/>
    <dbReference type="OrthoDB" id="130602at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="1">
        <location>
            <begin position="97"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="394" mass="47504" checksum="97157EED80DC226C" modified="2011-05-03" version="1">MGTSEFIKDIKCNRCGKQSKIFSEQILENKYCECGGKYEYNPEIDNFSDSDSINLTKNDLKREYQKKYDNYLELGKILKNDLEKSLKSKVDKFRIECRIKDFDKFYEKIQRKSYKNPFKEIEDICGIRIICYFSDDLEIISKIIKEEFDVKKSVNKSDLLDAEKFGYRSKHFIIKIKNDDLKQYEYQNLKDLTAEIQIRTLLMDVHASIQHKLTYKKGQYIPHKFQHQLAKLSAILELADEEFVRLKDDIWEYDYELKIVKENFLNENRKLKVDDFANIFRQELTIETLQKFLDFYFSDREKIFFKTKELYENIKRYNDENNDQITLNTLLELYYIIDDLEDLENKYFAQYENENKWHQSECINQILIIAHPKFKDYAANDSSKSTMTFRSTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z5</accession>
    <name>F0T5Z5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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        <reaction evidence="1">
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        </reaction>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_4_2"/>
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    <dbReference type="OrthoDB" id="89031at2157"/>
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    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REC"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="417" mass="46793" checksum="847A50837A09A6FB" modified="2011-05-03" version="1">MSNVKILVVEDESIVAMDIKHRAEGLGYSVTGITPSGEGAIQKATETKPDLVLMDIVLKGDMDGVEAAQRIRDSLDIPVVYLTAYSDEKTLKRAKVTEPFGYIIKPFEDRELHSAVEVALYKHKMESKLKESEEWLSTTLESIGDAVIATDDNGKIKFMNPVAMKITGWNEEESLGKDLKEVFQLVNETSGAPIDDPVNKIIDQNKILDFNEPALLVTKNGSKIPIDDSSAPITDKSGNIVGVALVFRDITERRKAEREREELLKEKARGELSNFVVSALPVFASNIPPQVRNNIAKSFSDRFEQNMKPAFDKEFAECTHDITDNKVLFNCYIDWLSDFLLNLGINTKKLEKDDNHCLQFINCPWLKEAGSSPMFCLICRAMVIRSFTWTNMRGNVEQTLCMADGAGKCNFEFKFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T621</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645879.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2314"/>
    <dbReference type="EnsemblBacteria" id="ADZ10528">
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        <property type="gene ID" value="Metbo_2314"/>
    </dbReference>
    <dbReference type="GeneID" id="10278780"/>
    <dbReference type="KEGG" id="mel:Metbo_2314"/>
    <dbReference type="HOGENOM" id="CLU_1998757_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="124" mass="14123" checksum="EA323CC4BD36065D" modified="2011-05-03" version="1">MRLSPIKSITLGFLTFFIIYIFIINLMIRLGFIFDNITLAFSLVVGSCIATYYTKEKKIQYGIYVGLIWAVLGLVPLLSFGFPADLSNLIINFLTFIKIIMMAIIGSYLAIVIGKHQKYKHENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T630</accession>
    <name>F0T630_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645888.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2324"/>
    <dbReference type="EnsemblBacteria" id="ADZ10537">
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        <property type="gene ID" value="Metbo_2324"/>
    </dbReference>
    <dbReference type="GeneID" id="10278790"/>
    <dbReference type="KEGG" id="mel:Metbo_2324"/>
    <dbReference type="eggNOG" id="arCOG13940">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714979_0_0_2"/>
    <dbReference type="OMA" id="FHSAAYE"/>
    <dbReference type="OrthoDB" id="62677at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="393" mass="42905" checksum="5E9A9CB919E4FD8E" modified="2011-05-03" version="1" precursor="true">MKKFTILVMMGLIFVGLLVIGLESSYSNNNTNQNYFQGLETNPSQATIMVLGSTYLYVPKNAKVVTQVDRVIKDVASNVNVNTPPGQSPNSSKQLVPYMEGARVAELMNYADVKVIPITGVPIKKIKGEWYGPDEAGNFQFGVDPSKAGSLFSIDGNDSNTKIEVNTHGMNVLVPGAIDNHAYLVVACGDLPGKAKAEKYLSDHGINCYAPCDRFSALVMPYNGTGKIMAGEPIRKCLTGGAVIGAQPLAIQTNETIVVQSTNKTYPDQYCDTPNRYFHCLESAYNMTLKLDVVDAGKGEAEKLINETKITGSKVIGVRVLTVQDKTAVANWLKSDPDNRAVLFHSAAYEPGYSLFTEFPNQVTGQDLNPVFIKQTPSDQLNHIFDEIRSLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T680</accession>
    <name>F0T680_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644746.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1151"/>
    <dbReference type="EnsemblBacteria" id="ADZ09395">
        <property type="protein sequence ID" value="ADZ09395"/>
        <property type="gene ID" value="Metbo_1151"/>
    </dbReference>
    <dbReference type="GeneID" id="10277601"/>
    <dbReference type="KEGG" id="mel:Metbo_1151"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168032_1_0_2"/>
    <dbReference type="OMA" id="TVWFTNK"/>
    <dbReference type="OrthoDB" id="113856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="11033" checksum="96310480890A2FC3" modified="2011-05-03" version="1">MTYMMIIKSVKDYDKWKSIFDEHENMRKSMGSRGASVLRNKNVPEQLVVIIEWEDMESAKKFVETEDLILAMEKAGVMGLPAVYYLEEKDRTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0T698</accession>
    <name>F0T698_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrG</name>
        <name evidence="12" type="ordered locus">Metbo_1169</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644764.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1169"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="ADZ09413">
        <property type="protein sequence ID" value="ADZ09413"/>
        <property type="gene ID" value="Metbo_1169"/>
    </dbReference>
    <dbReference type="GeneID" id="10277619"/>
    <dbReference type="KEGG" id="mel:Metbo_1169"/>
    <dbReference type="eggNOG" id="arCOG00063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="14061at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="6"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="305"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="506"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="537" mass="60370" checksum="3B886D4B9E9FF0AC" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6B7</accession>
    <name>F0T6B7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09432">
        <property type="protein sequence ID" value="ADZ09432"/>
        <property type="gene ID" value="Metbo_1188"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1188"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054151_0_0_2"/>
    <dbReference type="OrthoDB" id="25207at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="229"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="317" mass="34528" checksum="B7A7D5180AD8CB06" modified="2011-05-03" version="1">MLLLAFALILNVHTTSAATTNGTSSINSSVSSDVTNQVSAASSTSTTSKSKLVSTSKDVTKPKVVKTDPTKNAVKVDTKKTIKIYFSEAIKFGNGWIELKNNKKSVSIKTTINGKILTIDPLAYLSKDGNYNIYIHSGSITDMSGNKLSLYKTSFSTVSSKKPVSAAMQKYLVPTANCQSNNSKIKALAAQITKGSTTDYAKASKIFNWVRDHVSYSFYYNTQKGALGTLNSRSANCADTAHLVVALERAAGIPARYNHGTCRFSSGTWYGHVWAQVYVNGKWYYADGTSYRNSFGVVKNWNTKTFTLHGIYASLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C7</accession>
    <name>F0T6C7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09442">
        <property type="protein sequence ID" value="ADZ09442"/>
        <property type="gene ID" value="Metbo_1199"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1199"/>
    <dbReference type="eggNOG" id="arCOG14642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075387_0_0_2"/>
    <dbReference type="OMA" id="FAIWLLW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="5462" checksum="D4449FD9C79D9C1F" modified="2011-05-03" version="1" precursor="true">MVETQQASGGGLVGFAIIFLIILLLFPAFAIWLLYVAIVVMLIAGIISLIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D3</accession>
    <name>F0T6D3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="10">1.8.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1205</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the HdrA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644799.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1205"/>
    <dbReference type="EnsemblBacteria" id="ADZ09448">
        <property type="protein sequence ID" value="ADZ09448"/>
        <property type="gene ID" value="Metbo_1205"/>
    </dbReference>
    <dbReference type="GeneID" id="10277655"/>
    <dbReference type="KEGG" id="mel:Metbo_1205"/>
    <dbReference type="eggNOG" id="arCOG02236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="YCCMAAL"/>
    <dbReference type="OrthoDB" id="1148at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="245"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="579"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="608"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="777" mass="86149" checksum="B2FD10A6B182AD2C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I5</accession>
    <name>F0T6I5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate synthase</fullName>
            <ecNumber evidence="3">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645970.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2406"/>
    <dbReference type="EnsemblBacteria" id="ADZ10619">
        <property type="protein sequence ID" value="ADZ10619"/>
        <property type="gene ID" value="Metbo_2406"/>
    </dbReference>
    <dbReference type="GeneID" id="10278878"/>
    <dbReference type="KEGG" id="mel:Metbo_2406"/>
    <dbReference type="eggNOG" id="arCOG01602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_0_0_2"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="OrthoDB" id="76505at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="19360" checksum="C17263E4FE65AE1E" modified="2011-05-03" version="1">MRKDIRIAGFGGQGIILAGIVIGKAAALHDNIFAVQTQSYGPEARGGASRTEVVVSDEDVDYPKVQRPDIFVAMSHTALIAYLDDLKDGGTLIVDPDMITMDEIMPFVKEHNIQLYTAPATRTAAEEIGIRIVANIVMIGAIVEITKVVTEEAARAAIAESVPPGTEDKNLAAFDAGVKLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M4</accession>
    <name>F0T6M4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643608.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0004"/>
    <dbReference type="EnsemblBacteria" id="ADZ08257">
        <property type="protein sequence ID" value="ADZ08257"/>
        <property type="gene ID" value="Metbo_0004"/>
    </dbReference>
    <dbReference type="GeneID" id="10276426"/>
    <dbReference type="KEGG" id="mel:Metbo_0004"/>
    <dbReference type="eggNOG" id="arCOG13989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881076_0_0_2"/>
    <dbReference type="OMA" id="KQNEIME"/>
    <dbReference type="OrthoDB" id="131274at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="14591" checksum="C998D553B7D8178A" modified="2011-05-03" version="1">MTDWKSVGIGALANVVLTMILTIVLFPLFFLGPVIGGLLTAYLSNERLSQRTPASDAGDGALSGVIGGLLIGAIFILGFSVLSTVIGLVFTQIGAVLGTITLITGLFFTLITIILGGVLGAMGGVIGYSMREKEYVQVKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M6</accession>
    <name>F0T6M6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643610.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0006"/>
    <dbReference type="EnsemblBacteria" id="ADZ08259">
        <property type="protein sequence ID" value="ADZ08259"/>
        <property type="gene ID" value="Metbo_0006"/>
    </dbReference>
    <dbReference type="GeneID" id="10276428"/>
    <dbReference type="KEGG" id="mel:Metbo_0006"/>
    <dbReference type="eggNOG" id="arCOG10898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163807_0_0_2"/>
    <dbReference type="OMA" id="MTNWRAV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="12152" checksum="ABD4FB7C0D925C0E" modified="2011-05-03" version="1" precursor="true">MINWGAVILGFILSIILGGIFAVIVPIWGGILGLLLAGMAVGYMVGGDAMNGATNGALAGIFGAIVLSLLLLIAGTLILGIIGFAAASITSLFILAGFVGVMIIMAVGGIIGALIKDEPEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T2</accession>
    <name>F0T6T2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyrrolo-quinoline quinone repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643666.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0062"/>
    <dbReference type="EnsemblBacteria" id="ADZ08315">
        <property type="protein sequence ID" value="ADZ08315"/>
        <property type="gene ID" value="Metbo_0062"/>
    </dbReference>
    <dbReference type="GeneID" id="10276485"/>
    <dbReference type="KEGG" id="mel:Metbo_0062"/>
    <dbReference type="eggNOG" id="arCOG02482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_4_1_2"/>
    <dbReference type="OMA" id="GRVLWQR"/>
    <dbReference type="OrthoDB" id="26059at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="411" mass="44971" checksum="65D6A3E930A64047" modified="2011-05-03" version="1" precursor="true">MIGKKNILIGIMMLSIFIVPMTVSTASATDWPMFQENLNHTAYMDEASDFNPDTWLFQTSGLLTSAAITDKLIYFGSSSGLFSALNLEDGTKVWDYKAGGNITNAPIVVGDNVYFGSGDSYLYALNKKTGDDVWKYKTGNAIETTPAIDNGIIYFGSDDQRLYALNTNDSTKKWEFQTGNAVRSSPTVYNDTVYFGSDDGKIYALGIANGTQTWAYDTGSAVRSSPAINNTTLYMGTDNGNFYALNTADGSVLWNYNLNDSVKSSALLDPNDNALFIGSDNGNVTALDMRDGKLKWSVNTGNVKTTPALMGDNIVVTSETGTVYVLNKYSGKEEWSYTPGYYLFNSPLTSPIVYGDDIFVGDNNGNMYAIDFSRKTGPVSNYLYYVIAIVVVIVGGLLAVRTLRRRRKGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6T8</accession>
    <name>F0T6T8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643672.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0068"/>
    <dbReference type="EnsemblBacteria" id="ADZ08321">
        <property type="protein sequence ID" value="ADZ08321"/>
        <property type="gene ID" value="Metbo_0068"/>
    </dbReference>
    <dbReference type="GeneID" id="10276491"/>
    <dbReference type="KEGG" id="mel:Metbo_0068"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_3_0_2"/>
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    <dbReference type="OrthoDB" id="85871at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="23563" checksum="620DE6EC31F45022" modified="2011-05-03" version="1">MIFDPHIHSTYSSDSTASPRDIIKRAKKIGLDAIAVADHNTIKGSLVTIEEAKDFEDFVVVPAVEVSSSKGHIVALGIKEDVEKGLSPEDTVEKIRSLGGIAVAAHPFVSYRDGLCDSVKELDIDAIETLNSRYIFGYSNWKAKNLAERRNLPEIGSSDAHFIGAIGSCVTEFEADFSYESIIEAILSGKTNVYGDRTPLPLILKEVINKKIKKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V8</accession>
    <name>F0T6V8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09478">
        <property type="protein sequence ID" value="ADZ09478"/>
        <property type="gene ID" value="Metbo_1236"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1236"/>
    <dbReference type="eggNOG" id="arCOG02589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_2"/>
    <dbReference type="OMA" id="FEKFWFS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="13"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="16780" checksum="7FFDEF0504C2274A" modified="2011-05-03" version="1">MVNILNVVSDPIKILLVEDNPGDVRLIQEVFKDANICNAMEIAYDGETAMEILNSDNGHNFCPDLILLDLNLPKKDGREVLREIKNSDMLKCIPVVILTTSNAEDDLRETYNMNANCYITKPVDLDQFINVVKSIENFWLSIVKLPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T702</accession>
    <name>F0T702_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Energy-converting hydrogenase A, subunit K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644873.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1280"/>
    <dbReference type="EnsemblBacteria" id="ADZ09522">
        <property type="protein sequence ID" value="ADZ09522"/>
        <property type="gene ID" value="Metbo_1280"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_1280"/>
    <dbReference type="eggNOG" id="arCOG10247">
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    </dbReference>
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    <dbReference type="OrthoDB" id="123225at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="86" mass="9620" checksum="5DAAAABBAE9A1866" modified="2011-05-03" version="1" precursor="true">MDEQEKDVLFLMALAASGAVIASGLAAFQQWIVVLPLTVAVFILMILAGLQYKKRYIHLSENLENIAMILVLMGFIVSFIYLYRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T708</accession>
    <name>F0T708_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaE</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1286</name>
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1286"/>
    <dbReference type="EnsemblBacteria" id="ADZ09528">
        <property type="protein sequence ID" value="ADZ09528"/>
        <property type="gene ID" value="Metbo_1286"/>
    </dbReference>
    <dbReference type="GeneID" id="10277737"/>
    <dbReference type="KEGG" id="mel:Metbo_1286"/>
    <dbReference type="eggNOG" id="arCOG04830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191930_0_0_2"/>
    <dbReference type="OMA" id="LETQIWF"/>
    <dbReference type="OrthoDB" id="114920at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011317">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09880">
        <property type="entry name" value="DUF2107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036535">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="86" mass="9306" checksum="61C4C3A220E0ABE1" modified="2011-05-03" version="1" precursor="true">MLDVYVWFYTGCILVIVGSIATVIGPGVKDPVVRTINTEIPAVGISMIFLTYNHTIALVTFIAATAIITLILLRAVVRLEEMGAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T734</accession>
    <name>F0T734_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Butyrate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646019.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2455"/>
    <dbReference type="EnsemblBacteria" id="ADZ10668">
        <property type="protein sequence ID" value="ADZ10668"/>
        <property type="gene ID" value="Metbo_2455"/>
    </dbReference>
    <dbReference type="GeneID" id="10278927"/>
    <dbReference type="KEGG" id="mel:Metbo_2455"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="OMA" id="HAWSNLF"/>
    <dbReference type="OrthoDB" id="3223at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047760">
        <property type="term" value="F:butyrate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="41"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="465"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="557" mass="63188" checksum="383445120AA5BB77" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T761</accession>
    <name>F0T761_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2482</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2482"/>
    <dbReference type="EnsemblBacteria" id="ADZ10695">
        <property type="protein sequence ID" value="ADZ10695"/>
        <property type="gene ID" value="Metbo_2482"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2482"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_2_2"/>
    <dbReference type="OMA" id="CECGFFS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="33"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="39781" checksum="1C45AB2F56C72264" modified="2011-05-03" version="1">MRKHFSGYNFLADPETGVTFRWGNSFNEDPYMAPWPELVDISISNYCTNDCDYCYRQSSETGSFMSIEDFQTCLEQLNNTKFGSVFQIALGGGEPLLHPDFSKMLRLTREYNIIPNYTTSGKFFTEENLKTTRECCGAIAVSWDPTRNLTLDELKELGKLLKANDISSNIHYVVSNSTIETAIKILKGDLQEYLLNFNSVIFLTYKPTGRASNSDILQQGPELTDFLNLVDKSPTDLKIGFDACFVPVLIKHTAIDTDFVDSCECGFFSLYIDENLNVSPCSFCNDASYGYNLNKISIEDIWQEKLFDYREFVAENTKASCEDCDKNSECRGRCPFFKELFLCYDAEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T783</accession>
    <name>F0T783_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extracellular solute-binding protein family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646068.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2504"/>
    <dbReference type="EnsemblBacteria" id="ADZ10717">
        <property type="protein sequence ID" value="ADZ10717"/>
        <property type="gene ID" value="Metbo_2504"/>
    </dbReference>
    <dbReference type="GeneID" id="10278978"/>
    <dbReference type="KEGG" id="mel:Metbo_2504"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055936_0_0_2"/>
    <dbReference type="OMA" id="KDAPNRE"/>
    <dbReference type="OrthoDB" id="43889at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901359">
        <property type="term" value="F:tungstate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022498">
        <property type="entry name" value="ABC_trnspt_W-bd_WtpA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03730">
        <property type="entry name" value="tungstate_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="350" mass="37897" checksum="3F1848255CD8290C" modified="2011-05-03" version="1">MDKKIIIFVIVAIAIVGIGVYGYGVYSSGSENGTLVIYAASSLAGQMNQTAAKFESQHPNVKVQIKYGGSSDLINQITTLNQSVDIMASADYGLIDKNLMPNYTSYNLKNGRNEMVIAYTDKSKNSSQINGENWYQIFSQNNVSVGIADPNSAPAGYRGVMMMQLASTYYNDSTLFDTVIGQNTAITSEQNGSKTVINSPTNIDPTSKIVVRPAVGDLMPVLQSGAVDYILVYKSDADQQQSSGVKYVTLPPELALSNTTYEPVYKNISMVQFSGTNKSKTIGLTPIVYGITILNNAPNKQLATDFLQLLLSPEGIQITKSNFIDPISPAEATINSTNIPSQLQQYVKMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A4</accession>
    <name>F0T7A4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">F420-dependent methylenetetrahydromethanopterin dehydrogenase</fullName>
            <shortName evidence="5">MTD</shortName>
            <ecNumber evidence="2 5">1.5.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtd</name>
        <name evidence="6" type="ordered locus">Metbo_0080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:16721"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MTD family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.1" evidence="2 5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643684.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0080"/>
    <dbReference type="EnsemblBacteria" id="ADZ08333">
        <property type="protein sequence ID" value="ADZ08333"/>
        <property type="gene ID" value="Metbo_0080"/>
    </dbReference>
    <dbReference type="GeneID" id="10276503"/>
    <dbReference type="KEGG" id="mel:Metbo_0080"/>
    <dbReference type="eggNOG" id="arCOG04382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006890_0_0_2"/>
    <dbReference type="OMA" id="KGCFMTK"/>
    <dbReference type="OrthoDB" id="46875at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00695"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030268">
        <property type="term" value="F:methylenetetrahydromethanopterin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00058">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002844">
        <property type="entry name" value="MTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036080">
        <property type="entry name" value="MTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01993">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005627">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102324">
        <property type="entry name" value="SSF102324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="29273" checksum="5CFCD7310EAC5CDA" modified="2011-05-03" version="1">MVVKIGIIKCGNIGTSPVIDLLLDERADRPNIDTCVVGSGAKMNPEEIEKAVPLMLEMDRDFVIFISPNPGAPGPAKARELLSAADVPAIIIGDAPGLRSKDEMDEQGLGYIIVKADPMIGARREFLDPTEMASFNSDVIKVLALTGAYRVVQNTIDAAVTAVESGETLELPKVVITRDKAVEAAAFGSPYAKAKAMAAYEIATKVADIDVEGCFMVRDAEKYIPLVASAHEMLGVAAKLASEARDIEKANDTVVRTPHGGKGQTLSKVALADKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B2</accession>
    <name>F0T7B2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S26B, signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0088</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643692.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0088"/>
    <dbReference type="MEROPS" id="S26.017"/>
    <dbReference type="EnsemblBacteria" id="ADZ08341">
        <property type="protein sequence ID" value="ADZ08341"/>
        <property type="gene ID" value="Metbo_0088"/>
    </dbReference>
    <dbReference type="GeneID" id="10276511"/>
    <dbReference type="KEGG" id="mel:Metbo_0088"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_4_0_2"/>
    <dbReference type="OMA" id="WFPEPVI"/>
    <dbReference type="OrthoDB" id="106361at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="5">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15730" checksum="8AAB1273E6119521" modified="2011-05-03" version="1">MTDNREIISYVVIIVIGIILAQHMNVVVSGSMEPVFYRGDVVVIEKTDFLGIQEINKDDLKVGDIVIYQATWFPDPVIHRIIATGTDVNGTPYYVTKGDNNQVQDPAPVYHDQVMAKVVTLGNTPFVIPKVGYITLWIRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C2</accession>
    <name>F0T7C2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="16">BCAT</shortName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ilvE</name>
        <name evidence="17" type="ordered locus">Metbo_0098</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AL-21</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 16">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 16">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 16">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 16">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 16">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 16">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 15">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643702.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0098"/>
    <dbReference type="EnsemblBacteria" id="ADZ08351">
        <property type="protein sequence ID" value="ADZ08351"/>
        <property type="gene ID" value="Metbo_0098"/>
    </dbReference>
    <dbReference type="GeneID" id="10276523"/>
    <dbReference type="KEGG" id="mel:Metbo_0098"/>
    <dbReference type="eggNOG" id="arCOG02297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_1_2"/>
    <dbReference type="OMA" id="WRGSEMM"/>
    <dbReference type="OrthoDB" id="41340at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 16 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="14 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16 17" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ADZ08351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="34097" checksum="F527BB3BBDF72382" modified="2011-05-03" version="1">MAFDENGKIWFNGNFVDWKDANLHVLSHVVHYGTSVFEGIRCYDTKNGPAVFRLQDHIKRLINSGKIYRMEIPYSVEDLSQAVLDTIKINQLKECYIRPVAFRGYGELGVYPLNCPVETVVAAWAWGKYLGEEAIENGVNIGTSSWRRMAPGTMPNMAKAGSNYMNSQLAKMESIANGYDEAIMLDYQGMVSEGSGENIFVVLDGAIYTPPISSSLLSGLTRDSVIKIAQKLSLEVREENIPREMLYIADEVFLTGTAAEVTPVRSIDKIIIGNGTRGAVTKKIQENFFSLVNGEEEDEHGWLSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C8</accession>
    <name>F0T7C8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase RecJ domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643708.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0104"/>
    <dbReference type="EnsemblBacteria" id="ADZ08357">
        <property type="protein sequence ID" value="ADZ08357"/>
        <property type="gene ID" value="Metbo_0104"/>
    </dbReference>
    <dbReference type="GeneID" id="10276529"/>
    <dbReference type="KEGG" id="mel:Metbo_0104"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OMA" id="AVGDMQE"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="334"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="453" mass="50065" checksum="E29F1634D8A5548C" modified="2011-05-03" version="1">MIQKQQHSLLNKAEEACELLGRHLDQDHVVRIISHNDADGISAAGVMCNAVAKQQGKFHVTIIPRLKDENIAKFLKEKYKLFIFCDMGSGNLRGIKRLKGDVIIADHHQTIDSKEELSNSIVHVNPHLYELDGTKDVSGSGVSYLTVRPMGCKNLAGLALVGAFGDMQYKNGFTGVNKMIQDDGVDSGNIEIREDLKIAYRSNEPLYKALSYTINPVLKGISGDEEGAVTFLEKIGISYGIKFTELSNEEKDILKAELVKINPKIFGDVFTIPSEVPELTNLEEYSNILDACGKNKKQGIGLSICIGDRKDSIQEAQKLVKSYREDLVHGIQWIKKEGSVIQDNIQYIYTEEKRKKRIMGTLSSIGLDLEILDPGKPVLAMSRMDNIIKVSGRTTTEMTEMGVNLGYALENASKSFNGSGGGHNIAAGAVVPYREMDNFLNLVDEIVRTQLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D7</accession>
    <name>F0T7D7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein Metbo_0113</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643717.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0113"/>
    <dbReference type="EnsemblBacteria" id="ADZ08366">
        <property type="protein sequence ID" value="ADZ08366"/>
        <property type="gene ID" value="Metbo_0113"/>
    </dbReference>
    <dbReference type="GeneID" id="10276538"/>
    <dbReference type="KEGG" id="mel:Metbo_0113"/>
    <dbReference type="eggNOG" id="arCOG04308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165882_1_0_2"/>
    <dbReference type="OMA" id="RTIIWEV"/>
    <dbReference type="OrthoDB" id="116790at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9623" checksum="EBE4537B766DC1B9" modified="2011-05-03" version="1">MSQDAFERCNQILQHITEDTSVPRNIRRAAEESKELLSKDDDEPTVRASTVISILDEISNDPNIPIHARTLIWNVLSELESIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H6</accession>
    <name>F0T7H6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644895.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1302"/>
    <dbReference type="EnsemblBacteria" id="ADZ09544">
        <property type="protein sequence ID" value="ADZ09544"/>
        <property type="gene ID" value="Metbo_1302"/>
    </dbReference>
    <dbReference type="GeneID" id="10277753"/>
    <dbReference type="KEGG" id="mel:Metbo_1302"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065962_1_0_2"/>
    <dbReference type="OMA" id="YTELWAL"/>
    <dbReference type="OrthoDB" id="57453at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="282" mass="32771" checksum="F6DF5CF969EF759C" modified="2011-05-03" version="1">MNKQGISVIIHTLNEEKNLVNCLESVKWADDIVLIDNYSDDKTVEIAKSFTDRIFFCERVGFIEPARKFAVEQAKNEWVLIVDADEMVPLKMKQKLESIVADDLGDVVSIPHNNYFFGSKMERTGWGPLQDTHYRFYKKSLVELSDKIHSDPVIAESARIISIEDPDEGFVHFNYFTVEQFVEKMNRYTSIEAEGLFDAGDDLNSKQLALRVFDEFKLRYISFKGYKEGFRGLSMSLMMAAYRLTVLMKLKLMRNYSSKKPQKEVRKDYQKIADGIIEEYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M4</accession>
    <name>F0T7M4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1352</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644943.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1352"/>
    <dbReference type="EnsemblBacteria" id="ADZ09592">
        <property type="protein sequence ID" value="ADZ09592"/>
        <property type="gene ID" value="Metbo_1352"/>
    </dbReference>
    <dbReference type="GeneID" id="10277803"/>
    <dbReference type="KEGG" id="mel:Metbo_1352"/>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_0_2"/>
    <dbReference type="OrthoDB" id="89031at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="129"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="256" mass="29190" checksum="9D513795B9D9A9C5" modified="2011-05-03" version="1">MKKILIVEDEMITSLEMRMKIESWGYSVLDNVKSSSDAVETAIKNKPDLIIMDVVLYGNEDGITAAENIQKHVDIPIIYVTAYSSEMIMERAHKTSPYAYIIKPFDDNELKFAIELALKKQELKKEFDEKQELHSIISKNLGDVIWILDLKSAKFNYVSDSVEGLRGYTPDEVLEQSMEDVLTKESYQRIMKALPERIQSILSGDESLKVSKHLVDQTHKNGSKIPTEVVTSFLYNENGEVDRVLGVTRKLELPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7N9</accession>
    <name>F0T7N9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6 7">Histidinol dehydrogenase</fullName>
            <shortName evidence="6 7">HDH</shortName>
            <ecNumber evidence="6 7">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisD</name>
        <name evidence="13" type="ordered locus">Metbo_1367</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7 12">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1367"/>
    <dbReference type="EnsemblBacteria" id="ADZ09607">
        <property type="protein sequence ID" value="ADZ09607"/>
        <property type="gene ID" value="Metbo_1367"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1367"/>
    <dbReference type="eggNOG" id="arCOG04352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_0_2"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="2 6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6 8">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 8">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10 11">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ09607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="467" mass="50510" checksum="7B09F2F0524503E7" modified="2011-05-03" version="1">MATNKLLGDMYHNAVDNLQQLHIEIFNIEGVKYFSDKMKLVQLNDHKTQELIERSMLDENTAIDTVTAIINEVKNRGDEALGDYTKKFDGAELDTIKVDEETINSSLSKLDNDLVKALEQAASNIAKFHENQIPDEWSTEVDEGVTAGQIVRPLDSVGCYIPGGRAVYPSTILMTVIPAKIAGVKRIICCTPPQPDGTVKDVVLAAAKVAGATEVYMVGGAQAVAAMAYGTESIPRVDKIVGPGNIFVTAAKKLVYGQVDIDFPAGPSEVLIIADETADPAFIAMDIMAQAEHDPNAACALVTTSKKVAKQVDILIAEQLNNMERSEIITESLNSNGLIVVADIMEDAIKFSNAYAPEHLIIMTRDPEADLEEITNAGSIFLGELTPVAAGDYGSGTNHVLPTSGCARMYSGLSAESFVKKPTVQRLSEQGVLNLDEMVTKLAEYEGLHAHAESFKLRTEKVKKELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T4</accession>
    <name>F0T7T4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">leuC</name>
        <name evidence="8" type="ordered locus">Metbo_0169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643772.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0169"/>
    <dbReference type="EnsemblBacteria" id="ADZ08421">
        <property type="protein sequence ID" value="ADZ08421"/>
        <property type="gene ID" value="Metbo_0169"/>
    </dbReference>
    <dbReference type="GeneID" id="10276595"/>
    <dbReference type="KEGG" id="mel:Metbo_0169"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="OrthoDB" id="15714at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="7">
        <location>
            <begin position="8"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="416" mass="44564" checksum="A7867F517A00AABF" modified="2011-05-03" version="1">MGMTMAEKILAKASDKSVVEAGEIVMANIDVAMTHDLTGPLSVESFEKIGVEDVWDPEKIVVVFDHQVPADSLDAVGNHMIMRKFVEEQKINNFYDVREGVCHQVLPEKGHVVPGEVIVGTDSHTCTHGALGAFSTGIGSTDMAMVFATGKLWFKIPETIKFDITGKLKDNVFAKDVVLNIIGKVGADGATYKACEFGGEVTSAMSISDRMVMCNMAIEMGGKTGLVEPDQKTINYLKGRTTKNYEIMKTDNDAESLETVHINVDDLEPQIACPHNVDNVKPVSEVEGTHIDQIFLGSCTNGRISDLRTAAKIMKGKEVARGVRMLVIPASREIYTKALDEGLLRTFVDAGALVCNPCCGPCLGGHVGLIGPGEVSLSTSNRNFKGRQGSPEAEVYLSSAAVAAASAVTGKITDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U0</accession>
    <name>F0T7U0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0175"/>
    <dbReference type="EnsemblBacteria" id="ADZ08427">
        <property type="protein sequence ID" value="ADZ08427"/>
        <property type="gene ID" value="Metbo_0175"/>
    </dbReference>
    <dbReference type="GeneID" id="10276601"/>
    <dbReference type="KEGG" id="mel:Metbo_0175"/>
    <dbReference type="eggNOG" id="arCOG01116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065511_0_0_2"/>
    <dbReference type="OMA" id="NKTQNCV"/>
    <dbReference type="OrthoDB" id="67932at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017674">
        <property type="entry name" value="Methan_mark_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03280">
        <property type="entry name" value="methan_mark_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1743" evidence="1">
        <location>
            <begin position="160"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="310" mass="34062" checksum="BB9F5AEDCD56DF09" modified="2011-05-03" version="1">MEILTPENLKDKFKDPWIAPYKKVITMVDKDRVELVEYHPCVAGSEWMVYQYKRTSDLVTESKRDGDKHIFQLKVGKADMELKPSYSAAGIEEVVVDDDEVKVVHAGLAGAGVGAAMCRGMAKGVKRVELYDIGGGSKVGRAAVVTPKLQKVIVGIDDTDTKEEGATWTLANNIGIELSKRGFEYLDHVTCQLYPHNPNKTQNCVAIALVFAVKPEMKEEMINKICEILKRDTLSDKTAIAVMDGFDVPDVLREYGEATKKSMMTVEDAENVAKKAGVKLIQVTGAPGEIGALAALGLYNDLEEAVKVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7W7</accession>
    <name>F0T7W7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643805.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0202"/>
    <dbReference type="EnsemblBacteria" id="ADZ08454">
        <property type="protein sequence ID" value="ADZ08454"/>
        <property type="gene ID" value="Metbo_0202"/>
    </dbReference>
    <dbReference type="GeneID" id="10276628"/>
    <dbReference type="KEGG" id="mel:Metbo_0202"/>
    <dbReference type="eggNOG" id="arCOG01075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_3_2"/>
    <dbReference type="OMA" id="NRDPRGH"/>
    <dbReference type="OrthoDB" id="116493at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
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        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="14865" checksum="92C78DFEF68271A6" modified="2011-05-03" version="1">MKTPLLTVDTVVVQNSSILLIKRKNDPYQGSWALPGGFVEYGETVEDAAVRETKEETGIDVILKELVGVYSDPDRDPRGHTVTVCFLGSKIGGMLKSATDADDAKYFDLNEIKTLDLAFDHERIIHDSLKLLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T826</accession>
    <name>F0T826_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1413"/>
    <dbReference type="EnsemblBacteria" id="ADZ09652">
        <property type="protein sequence ID" value="ADZ09652"/>
        <property type="gene ID" value="Metbo_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1413"/>
    <dbReference type="eggNOG" id="arCOG06481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036670_0_0_2"/>
    <dbReference type="OMA" id="PYRWYEY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041113">
        <property type="entry name" value="Heliorhodopsin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18761">
        <property type="entry name" value="Heliorhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="31764" checksum="FD44445EEE63969F" modified="2011-05-03" version="1">MFLDNDMRRELIAKSSITFSSLRKLNIGAGIFLFVQGILMVVLGYLLTWNKDIYTFYLKFKIISVVPPSFSIQPDPQVVYTLTHLGVILSSFLLISGLALLLIGFVKYKPYVENLKKGMNPYRWYEYAITSSIMLVIIATFVGVWDLWSLVMIFVLNATMILCGYLMEKINFHTKKTDWSAYLLGCLAGFTPWIVLAAYFIAELGSSQTKPPAFVYYILLFYFILFNTFSINMILQYKGVSKWKDYLYGERVYIILSFVAKTMLAWIVFFGIFAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T837</accession>
    <name>F0T837_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1425</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645014.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1425"/>
    <dbReference type="EnsemblBacteria" id="ADZ09663">
        <property type="protein sequence ID" value="ADZ09663"/>
        <property type="gene ID" value="Metbo_1425"/>
    </dbReference>
    <dbReference type="GeneID" id="10277876"/>
    <dbReference type="KEGG" id="mel:Metbo_1425"/>
    <dbReference type="eggNOG" id="arCOG01961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018415_1_0_2"/>
    <dbReference type="OMA" id="PAILMCF"/>
    <dbReference type="OrthoDB" id="22707at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="10"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="400" mass="41944" checksum="D50AEB706DFB4728" modified="2011-05-03" version="1">MDNILLSLGIIIVFGLIFSRLFSKIGLPGLLGMLVVGVLIGPYYLNLIDPRFLGISSDLRVMALIIILLRAGLSLHRDALRKVGTSAIKMSFLPCLLEGLTIMAVAHLLLGLPWIVAGMLGFIIAAVSPAVVVPSMLSFIERSMGTAKSIPTLLMAGASADNVVAITIFSAFFGFYLNSSLNIGIAIIGIPVSLILGMIMGFLLGLLILRVFQHYSIGSSEKILIILASAILLKELGDYLQNYVPVAALLGIMVLGFVIVDRNPNLGLELSSKFSKIWILAEIILFVLVGAQVNIQLVISAGVAGIIIIGVGLLARSLGVYLSLSGTELNKDEKLFSMVANTPKATVQAAIGAVPLAAGVASGDIILAISVLAIILTAPLGSLGIKVLGDKILKKEPSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T845</accession>
    <name>F0T845_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645022.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09671">
        <property type="protein sequence ID" value="ADZ09671"/>
        <property type="gene ID" value="Metbo_1433"/>
    </dbReference>
    <dbReference type="GeneID" id="10277884"/>
    <dbReference type="KEGG" id="mel:Metbo_1433"/>
    <dbReference type="eggNOG" id="arCOG04854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772958_0_0_2"/>
    <dbReference type="OMA" id="TIDYMLP"/>
    <dbReference type="OrthoDB" id="69327at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="362" mass="40161" checksum="56867536AC0D9C5D" modified="2011-05-03" version="1">MAFIAVNNLEFILEVAIFLQLAIILVLNLFRITLSRILFFSLILGIGLTALFSFDAMALFLPFLGLHEFTHTYGPIAILVIVTAWAALSTMSEVGIQVMNVKRLVFLMIILITIVGGLVHRDFLILWMMGLFFGFFFISRSFRQKSFLTAKRVIALVAAVVVGFTSLELLSRLLSMTILSPIVRIERIFDNAIPSLKMVISNTTLWGHNPGSSYWNTTDTGSSSGYIALPISLILTFGLPYQIFFGVLVTKKDIIDYFVPGIFGFAFDFGYIVLALLLIWCIFILVLGMKILSEYRAKREKGNKTLLGREALLIGSLAAFASQAILGLFIINRAINGTALVTFIFLSGLVLAHVILPKNTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B6</accession>
    <name>F0T8B6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643879.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0276"/>
    <dbReference type="EnsemblBacteria" id="ADZ08528">
        <property type="protein sequence ID" value="ADZ08528"/>
        <property type="gene ID" value="Metbo_0276"/>
    </dbReference>
    <dbReference type="GeneID" id="10276708"/>
    <dbReference type="KEGG" id="mel:Metbo_0276"/>
    <dbReference type="eggNOG" id="arCOG02232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="OMA" id="PFVKGMY"/>
    <dbReference type="OrthoDB" id="43345at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="427" mass="46320" checksum="E7D0ECD2AB4C8332" modified="2011-05-03" version="1">MKVVVVGSGAGGASAAMELSKNGHKVTILESGKPFKPFTRLMTLTEPLKKLGLLGNESNIMRIFPPMNVARSSQKLVVVRGITQGGCTAISCGNMVRAEHGLKELGLDLTPEFETIENQTSINSVPKNRWRPLTQKMFESAEELNLKPSPTPKAVEFHKCTSCGLCEMGCQTGARWDSGKLLDIAIENGAILHTSSKVQEILTRNNQVYGVSVERGKSNQVYPADAVVLAAGGVGTAQILKKSGIYCEDKLWVDIVLTLGGVAKNSKQLREPPMAWYSSQKDYILSPYIDILSHYFHKPWRDVSLKDRVGIMVKLADVENGAVFEDGTIEKEVTNTDEAKLDKAILKAKNIMESSGVSGPFVKGMYNGGHLGGTVPLKKKDVSSMRSSELPDGLWIADLSLAPQSQGLPTIMLTSALAMRVSKKICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C4</accession>
    <name>F0T8C4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643887.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0284"/>
    <dbReference type="EnsemblBacteria" id="ADZ08536">
        <property type="protein sequence ID" value="ADZ08536"/>
        <property type="gene ID" value="Metbo_0284"/>
    </dbReference>
    <dbReference type="GeneID" id="10276716"/>
    <dbReference type="KEGG" id="mel:Metbo_0284"/>
    <dbReference type="eggNOG" id="arCOG11509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102544_0_0_2"/>
    <dbReference type="OMA" id="CDTLDGP"/>
    <dbReference type="OrthoDB" id="92223at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045613">
        <property type="entry name" value="DUF6448"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20046">
        <property type="entry name" value="DUF6448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="19172" checksum="C283C839CEE5006F" modified="2011-05-03" version="1">MTIYYDTMDGPIVTAAELALEMENVNYVLPFVKKEFETELKEAFDNAVIVRELSGEAAEVADQWFFETAVRLHMVGVGKPYTGIKHSGLNREHIILKAYSAIENQDKTALETLLSDSLREAIETRFEVTISKKDYDVNDIDAAREYMNFERDFVEFCNELLELIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D1</accession>
    <name>F0T8D1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643894.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0291"/>
    <dbReference type="EnsemblBacteria" id="ADZ08543">
        <property type="protein sequence ID" value="ADZ08543"/>
        <property type="gene ID" value="Metbo_0291"/>
    </dbReference>
    <dbReference type="GeneID" id="10276723"/>
    <dbReference type="KEGG" id="mel:Metbo_0291"/>
    <dbReference type="eggNOG" id="arCOG04875">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113977_0_0_2"/>
    <dbReference type="OMA" id="FIIEHFD"/>
    <dbReference type="OrthoDB" id="71402at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="194" mass="21844" checksum="33D3B6951ECE61AF" modified="2011-05-03" version="1">MKHLKLENETCYDGSQIEPYWAFKTFGIKDSSIVSWTGPMEIHPNNLIDFEDIGIEIKGDNMLHFIIEHFDSQPADLNLCYHRQRIFVLIVKDLLNDWGMVTSREGDDLFFDKGNIKGKLSVSIATCSVSSMKIHFALNLTENGTPADVETAALLEAGLSIEASHVEEIAEIACDKYIQEISDIKSDITKTKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I4</accession>
    <name>F0T8I4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NERD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1503"/>
    <dbReference type="EnsemblBacteria" id="ADZ09735">
        <property type="protein sequence ID" value="ADZ09735"/>
        <property type="gene ID" value="Metbo_1503"/>
    </dbReference>
    <dbReference type="GeneID" id="10277954"/>
    <dbReference type="KEGG" id="mel:Metbo_1503"/>
    <dbReference type="eggNOG" id="arCOG06670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088143_0_0_2"/>
    <dbReference type="OrthoDB" id="79383at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08378">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50965">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="NERD" evidence="2">
        <location>
            <begin position="127"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="299" mass="33797" checksum="24155CE35B88D9C9" modified="2011-05-03" version="1">MVSNKEIVDKYDNGLLVCLNCEYSYALEPGESPDDFILKCSCGGKLNYQKSEREIQTRTKGFAEKKSDQNKFKKRLGLYSCTLGAVVFVGSIIMSQVVLGMIALFLSVFVGTRLYSSGVENGKRWNKGILGEKRVIKYLNMLPEAYFVFNDVKFPGSYGDLDHIVVGPTGIFVIETKNYQGSYVVKEDGWYYQNGEGIKKSKGQPGKQVMRNAMSLKRFMATNNINMKGVWIDPIVTLVNNNFEIIEKPEYYNVLSPSAIPKFIVNKKNHTAPSILQRTIDLIEPYCTELSYLEEDRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J3</accession>
    <name>F0T8J3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645095.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09744">
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        <property type="gene ID" value="Metbo_1513"/>
    </dbReference>
    <dbReference type="GeneID" id="10277964"/>
    <dbReference type="KEGG" id="mel:Metbo_1513"/>
    <dbReference type="eggNOG" id="arCOG11271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933267_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="13836" checksum="FA8C1F668E4E270F" modified="2011-05-03" version="1">MNDLKNDLKYIILGIVLAFIITLGFTKIFMGGLYFIFASFLITGIIVGYLTSGDTKDVAINGMVVGFIGSIIFILFVSIMTMLTSTPHMSTSIVIMSIGGTLMFGIVAGVIFGIICAIGSIIGSYIKSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J5</accession>
    <name>F0T8J5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1515</name>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1515"/>
    <dbReference type="EnsemblBacteria" id="ADZ09746">
        <property type="protein sequence ID" value="ADZ09746"/>
        <property type="gene ID" value="Metbo_1515"/>
    </dbReference>
    <dbReference type="GeneID" id="10277966"/>
    <dbReference type="KEGG" id="mel:Metbo_1515"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="FINTASW"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="286"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="385" mass="40105" checksum="7A720F3C32532F0C" modified="2011-05-03" version="1">MSFLSLVVKNPFRNKTRSSLAIVGIAIGIMVIVALGMITGGLENSTQSTLKAGSAEITVSQAGSSNMQSGQTINQTYVNDLLPLSGVKSTAGVLRATNTTSGASSSNTSQAGGFGGLSITGVDSDKLSLLGVDSVNGTAFTNDSTDQVIIGKTAAESLNKTVGDTIDLFGKNFTVKGTFETGSFMTDNGIMMPLSTLQNLTSNQDKVSSVLVKVTDNANVTTVSNTIQDAYPNQLTTSTAADQANRINQGLGFINTASWAISLLAIFIGAVGVINTMIMTVYERTREIGVLKAVGWKDTRILGMILGESIVLTLLAFVAGTLIAVVGVEVLLTLVPSVGSVITPSFSIYIFLRAFAVALVVGVIGGLYPAYRASRLSPTEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T902</accession>
    <name>F0T902_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645181.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09830">
        <property type="protein sequence ID" value="ADZ09830"/>
        <property type="gene ID" value="Metbo_1603"/>
    </dbReference>
    <dbReference type="GeneID" id="10278060"/>
    <dbReference type="KEGG" id="mel:Metbo_1603"/>
    <dbReference type="HOGENOM" id="CLU_1830607_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="150" mass="16636" checksum="90FA813F1D2A406D" modified="2011-05-03" version="1" precursor="true">MFNTQGNSKVYKIIPLMVAALILVSSISSVAAEDPKQTHIDGVTHVNCEQGDVVTITAQLWITNYLWDNPLTGEDLYFYVYSGISDDNNIFFHRTTETSWPTGEASVQIDTKNINPGNYTLLVHYDGGGDLFGYDGCSKETTFTVRPKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T909</accession>
    <name>F0T909_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L10e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl10e</name>
        <name evidence="6" type="ordered locus">Metbo_0379</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643982.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0379"/>
    <dbReference type="EnsemblBacteria" id="ADZ08631">
        <property type="protein sequence ID" value="ADZ08631"/>
        <property type="gene ID" value="Metbo_0379"/>
    </dbReference>
    <dbReference type="GeneID" id="10276816"/>
    <dbReference type="KEGG" id="mel:Metbo_0379"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OMA" id="HHVIREN"/>
    <dbReference type="OrthoDB" id="82104at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_L10e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_L10e_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="PTHR11726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L16" evidence="5">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="18075" checksum="ECDF735253D77244" modified="2011-05-03" version="1">MVRAYTRKDYIRKIPGSRIVQYDMGNLSGDFPLKVTLAVKEHAHLSHNALEAARIASNRYMQRKAGRMGYHLKIRVYPHHIVRENPMATGAGADRVQDGMRKAFGKPVSSVAIVKPNQKVLTVFTNKRNFNDAKEALRRAAMKFPVTCRIEVEEGADLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T950</accession>
    <name>F0T950_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thg1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0420"/>
    <dbReference type="EnsemblBacteria" id="ADZ08672">
        <property type="protein sequence ID" value="ADZ08672"/>
        <property type="gene ID" value="Metbo_0420"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0420"/>
    <dbReference type="eggNOG" id="arCOG03218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044271_3_1_2"/>
    <dbReference type="OMA" id="WKQHTEI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="PTHR12729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thg1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="256" mass="30310" checksum="8EBDB7BC56DE5D70" modified="2011-05-03" version="1">MVFRMKECEIFSNLRVPCGSEVVVRVDGRKFSNLSAELELEKPYDQDFTNLMCEVCSEFFKEFAPKFIYTFSDEINILLSDIPFNGRIEKLNSVFAGFISASFQKNLLKIEKFKEIIDEHDIKPVSFDSRIIPLALEGTIDYFKNRQSESWRNCINGYSYWKLRENHDKTSAVEILNKKKSSELHDLLFENGLNISEVPIWQRRGIGIYRTANEVEGYNPVENKRVKTMRYLPMVDRELPIINREFFENRIFYWYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T997</accession>
    <name>F0T997_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chloride peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.11.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1621"/>
    <dbReference type="MEROPS" id="S33.991"/>
    <dbReference type="EnsemblBacteria" id="ADZ09848">
        <property type="protein sequence ID" value="ADZ09848"/>
        <property type="gene ID" value="Metbo_1621"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1621"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_12_3_2"/>
    <dbReference type="OMA" id="EGQIRSW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016691">
        <property type="term" value="F:chloride peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="26"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="30592" checksum="60FE18CF9A5DDFD9" modified="2011-05-03" version="1">MQYIEVGKENSKKIKLHYEDHGSGDPVLLVHGWPLNGASWEKQELALLNSGYRVITYDRRGFGDSSQPLEGYDYDTFAADLDKVIKTLELEDVTLAGFSMGTGEVTRYIGTYGTDNIKKACLLASIPPFLLKTSDNPDGVDGEGFNQIKAAIKGDRLAFLAQFLNDFYNMDLLGGKLVSKEVFQNSWNVASKASPTGTYECVDAWLTDFRKDVDAIDIPTLILHGDADRILPIDATARKLKNLIKNNKYVEIENGPHGLIWTHAEQVNQAILDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T999</accession>
    <name>F0T999_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1623"/>
    <dbReference type="EnsemblBacteria" id="ADZ09850">
        <property type="protein sequence ID" value="ADZ09850"/>
        <property type="gene ID" value="Metbo_1623"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1623"/>
    <dbReference type="eggNOG" id="arCOG04946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025311_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="30651" checksum="28AF79DE7A580002" modified="2011-05-03" version="1">MSRVIHFEIPAEDPKRAINFYEQVFSWEIQKWEHGDYWLVSTGSEEENGINGAIYPKETGSTVRDTINVESYEEFAKKIEANGGKMLTDKMPIPGMGFNGLFRDTEGNVFGIIEVTMVYISRLFNAKIEDVWEAWTEPEHVKEWLAPKNYTAPLVKMDLQVGGSYLNSMKSSEGDEVWSTGVYREIVPKEKIVSTDSFADKDGNVVPASHYGMGEDWPMELTVTVTFNEEDGATRLTIEHQGFPDHHNSAMAETGWQESLDKLEAHLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B2</accession>
    <name>F0T9B2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645214.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09863">
        <property type="protein sequence ID" value="ADZ09863"/>
        <property type="gene ID" value="Metbo_1637"/>
    </dbReference>
    <dbReference type="GeneID" id="10278094"/>
    <dbReference type="KEGG" id="mel:Metbo_1637"/>
    <dbReference type="HOGENOM" id="CLU_1529243_0_0_2"/>
    <dbReference type="OrthoDB" id="138818at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="175" mass="20633" checksum="DE7EB5F494939928" modified="2011-05-03" version="1">MSNFKNLKKVIETNVESVHKDFKLQPSEIGKPSTKTDLNKVDVSKVPKEQRDKLDSYIQQWENPSKWYECRIQGLEWFLSIAQKNNVSPENIEFVIEENSQKKPRLTPMLKSMEVSSQIIPKTDDMDRLRKFLKLYPKYRIRVENGENVVDMAISIIKSYVEKEERRNLGLDNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D0</accession>
    <name>F0T9D0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645232.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1655"/>
    <dbReference type="EnsemblBacteria" id="ADZ09881">
        <property type="protein sequence ID" value="ADZ09881"/>
        <property type="gene ID" value="Metbo_1655"/>
    </dbReference>
    <dbReference type="GeneID" id="10278112"/>
    <dbReference type="KEGG" id="mel:Metbo_1655"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061526_0_0_2"/>
    <dbReference type="OMA" id="CATGPCA"/>
    <dbReference type="OrthoDB" id="45763at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="29105" checksum="9BE0192FA012B396" modified="2011-05-03" version="1">MFLEELELKIGKFIETSDMNLVEDLDGLQIFDKPLIGVAASDDSLWNTLKKPEVVGPDHMAPTEWMSESKSVLSYFLPFTEAVRKSNRTKGMPSIEWLYGRWDGELFNNALRSFMVESLEDQGFRAVAPALNEGFSVENHKSNWSERHVAYIAGLGTFSINRSLITNRGTAGRFGSVITDWELEPKPRDYTEVNEYCDGCGSCISRCPCKAITMETMDKEQCFGFLNKILKRNHPRYGCGKCQTAVPCEFTNPTTHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H7</accession>
    <name>F0T9H7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0473"/>
    <dbReference type="EnsemblBacteria" id="ADZ08725">
        <property type="protein sequence ID" value="ADZ08725"/>
        <property type="gene ID" value="Metbo_0473"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0473"/>
    <dbReference type="eggNOG" id="arCOG01153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063034_1_0_2"/>
    <dbReference type="OMA" id="PNVWMIE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="28053" checksum="9C82E1FB4F7FC333" modified="2011-05-03" version="1">MELKNNLLRQVTDENPDLIIVSGDLTTEGYSHEYDDAAEFVDELNSISEVHVVPGNHDARNVGLIHFEKQIGERKFIHIDKSGGFVIAGLDSSEPDINDGQIGYDQLAWLKSELEKVPKDMCKIVTFHHHLLPIPQTGRERNILLDSGDLLRLFIDSGVDFVLNGHKHVPNVWMIEKMVTLNSGTATTRKLRGNTYPSYNELLIDDGGIFVNLINTFTGYKREVANYAVELNENEYSICSYNYNSDNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I9</accession>
    <name>F0T9I9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644088.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0485"/>
    <dbReference type="EnsemblBacteria" id="ADZ08737">
        <property type="protein sequence ID" value="ADZ08737"/>
        <property type="gene ID" value="Metbo_0485"/>
    </dbReference>
    <dbReference type="GeneID" id="10276925"/>
    <dbReference type="KEGG" id="mel:Metbo_0485"/>
    <dbReference type="eggNOG" id="arCOG01502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="VPVMCHQ"/>
    <dbReference type="OrthoDB" id="105930at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12798">
        <property type="entry name" value="Fer4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15801" checksum="FD14CF0D6CAEB253" modified="2011-05-03" version="1">MRELISKPELCDECSRCERECPQNAIRVISGVPVHCLHCAKDRAPCMTVCPEDAIVEIDGAIVIMEDSCIGCGLCRDSCPIGAIHMDEYGIAKKCNLCIDKETPACVLTCPKDALKVDSEDILAQKRDKIAEELSRVKLIMKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K1</accession>
    <name>F0T9K1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c biogenesis protein transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644100.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0497"/>
    <dbReference type="EnsemblBacteria" id="ADZ08749">
        <property type="protein sequence ID" value="ADZ08749"/>
        <property type="gene ID" value="Metbo_0497"/>
    </dbReference>
    <dbReference type="GeneID" id="10276937"/>
    <dbReference type="KEGG" id="mel:Metbo_0497"/>
    <dbReference type="eggNOG" id="arCOG02398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053225_4_1_2"/>
    <dbReference type="OMA" id="PMVPFYL"/>
    <dbReference type="OrthoDB" id="91633at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="202" mass="22493" checksum="2489F61EDDFA7345" modified="2011-05-03" version="1">MVLGYVASFSAGILSVISPCVIPLIPIVVGHSLLKRDYNEILTFTSGFFLVFAVLTLLTGIFTLAIAHYLLYFRMAAAILIILMGIIFMLNKNIFNFSYRLKHENQRFESFIAGILTCVAWSPCYGPYIVAVAAYSASTGNWFYSVSNMIIYSLGFSFSIFILALLISRINIEGLMKHYNTIRMVSGAMIIIAGIYLLTGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K5</accession>
    <name>F0T9K5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MATE efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644104.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0501"/>
    <dbReference type="EnsemblBacteria" id="ADZ08753">
        <property type="protein sequence ID" value="ADZ08753"/>
        <property type="gene ID" value="Metbo_0501"/>
    </dbReference>
    <dbReference type="GeneID" id="10276941"/>
    <dbReference type="KEGG" id="mel:Metbo_0501"/>
    <dbReference type="eggNOG" id="arCOG01731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_1_2"/>
    <dbReference type="OMA" id="SKAIMHM"/>
    <dbReference type="OrthoDB" id="52466at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="475" mass="50478" checksum="0B601902E92B71A3" modified="2011-05-03" version="1">MNKSDTNMESSKSGVDKRVEMITGDPKRAIRTLSVPMILSMLLMMAYNLADSIWVAGLGPNALAALGFVSPLFMMVVGLGNGLGAGANSLISRCIGADNKRGSDNAAIHSLILAFAVSLLLTLLLLLVLPIILRIMGVGPAFDLAMQYGEIVFGGLIFMILANVASGVLRAEGDVKRPMYAMMATATLNIVLDPIFIYYFGLGVAGAAWATVISSAISSAIIMYWLLLKKDTYVSLSKKDFTASWKVVKNILSVGIPASAEFFVMSLLGIFVNTMLVFAGGTEAVAVYTAGWRVVMIAMIPPIGIGTAAITVAGAAYGARRYNHILTALNYSVKLAVAIATVLSLGIFIFASSIATIFSYSAQSSFLAPSIASFLQVMCLFLITVPLGISASSVFQGMGKGLTSLILTALRELIFILLFSYLFGMVLNFGVHGIWWGIVLGGAIGCLIGYLWALRYIRGLIKSYKNNKSPGEASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L3</accession>
    <name>F0T9L3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0251 protein Metbo_0509</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0251 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0509"/>
    <dbReference type="EnsemblBacteria" id="ADZ08761">
        <property type="protein sequence ID" value="ADZ08761"/>
        <property type="gene ID" value="Metbo_0509"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0509"/>
    <dbReference type="eggNOG" id="arCOG02238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094511_0_0_2"/>
    <dbReference type="OMA" id="ECAQKMQ"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00674">
        <property type="entry name" value="UPF0251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002852">
        <property type="entry name" value="UPF0251"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478">
        <property type="entry name" value="PTHR37478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02001">
        <property type="entry name" value="DUF134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="23193" checksum="65D9648A75101089" modified="2011-05-03" version="1">MNIYSFMVRPQVFRKISKNPEVRCFKPDGENLEELEPIQISLDEFEAIRLRDYHDIKQVKAASIMEISQPTFHRILSSARKKISKALIEGNPIIIVGDGFITDKKRYKCNDCGFEWLSNEKNYDKCLECQSENIVKTDEEDMLIKIDPDILERRSYGGKGLGAGPPKVCKCPKCGYEHPKTRTIPCRNTKCPVCETPLCGSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P8</accession>
    <name>F0T9P8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type metal ion transporter, periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645278.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1702"/>
    <dbReference type="EnsemblBacteria" id="ADZ09927">
        <property type="protein sequence ID" value="ADZ09927"/>
        <property type="gene ID" value="Metbo_1702"/>
    </dbReference>
    <dbReference type="GeneID" id="10278159"/>
    <dbReference type="KEGG" id="mel:Metbo_1702"/>
    <dbReference type="eggNOG" id="arCOG01005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_0_2"/>
    <dbReference type="OMA" id="FHEAFDY"/>
    <dbReference type="OrthoDB" id="59250at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006129">
        <property type="entry name" value="AdhesinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00691">
        <property type="entry name" value="ADHESINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="32723" checksum="864869CBFB60A9AC" modified="2011-05-03" version="1" precursor="true">MKKKRVIVVIIILLISAIVLGYLAQQKHSSKTENNKIGVVVSLGPEVEWVKAVGGSKVDVTLMVPEGSDPHTYEPLPNQLSQVSNAQIYVMMGSSVEFENNYMDKIRETNPNLLVVNASEGVKLIPNSAENESDTVDPHVWADPKNAKIMVNNIYNGLVEVDPSDKDYFTKNRDAYLQELDELDKNTTKLLKDKNNSYILVLHPAFGYMARDYNITEIGAMINDEEPSPQRIAMMINTAKEYNITTVYNEPQYDPKFMESIASQIGGKVVTVNDLGENYLQNMANIAVAFSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U8</accession>
    <name>F0T9U8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645328.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09977">
        <property type="protein sequence ID" value="ADZ09977"/>
        <property type="gene ID" value="Metbo_1754"/>
    </dbReference>
    <dbReference type="GeneID" id="10278211"/>
    <dbReference type="KEGG" id="mel:Metbo_1754"/>
    <dbReference type="eggNOG" id="arCOG01778">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062440_1_0_2"/>
    <dbReference type="OMA" id="CYTFFKA"/>
    <dbReference type="OrthoDB" id="49237at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="36"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="267" mass="29574" checksum="005E29949900B91F" modified="2011-05-03" version="1">MEDEYVHGYSEIESDRLVDQANTLANLLHNDTVYSAGSKVLEAGCGVGAQTLMLATNSPDAQIKSVDISEESVNRAKLLLKQHGVSNVEFEVADIFNLPFEAETFDHIFVCFVLEHLKDPVGALEGLRRVLKMGGSITVIEGDHGSCYFYPKTKEAVKAWECLVKCQTGLDCNPMIGREIYPLLKNSGFKNIEVSPKIVYVDSSNPELVEGFNKKTIIAMVEGVKDQAISSGMIDLETWEKGIKDLYKTTEPDGVFFYNFFKGIGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X0</accession>
    <name>F0T9X0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-binding protein Metbo_0541</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0541</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644144.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0541"/>
    <dbReference type="EnsemblBacteria" id="ADZ08793">
        <property type="protein sequence ID" value="ADZ08793"/>
        <property type="gene ID" value="Metbo_0541"/>
    </dbReference>
    <dbReference type="GeneID" id="10276986"/>
    <dbReference type="KEGG" id="mel:Metbo_0541"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OMA" id="KPQFGEQ"/>
    <dbReference type="OrthoDB" id="130316at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PTHR10840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="SSF46950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="13726" checksum="F5FDF6A88DE7EA9F" modified="2011-05-03" version="1">MTDIEEIRRRRMQELQQQQAQQAQNQSSQAESQEQMRREVEAQKRQAMMQLLTPEARARLTNIRLTKPEFVDQIELQLIQLAQMGRVQNKITDDQLKELLRKLAGQKREINITRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z8</accession>
    <name>F0T9Z8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0570</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644172.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0570"/>
    <dbReference type="EnsemblBacteria" id="ADZ08821">
        <property type="protein sequence ID" value="ADZ08821"/>
        <property type="gene ID" value="Metbo_0570"/>
    </dbReference>
    <dbReference type="GeneID" id="10277015"/>
    <dbReference type="KEGG" id="mel:Metbo_0570"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_0_2"/>
    <dbReference type="OMA" id="MACWNSN"/>
    <dbReference type="OrthoDB" id="66605at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="PTHR43029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="12"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="42633" checksum="2FDBD24B51464081" modified="2011-05-03" version="1">MVEAILNSGDTAWMLTSTALVMLMTLPGVALFYGGLTKKENVLNTIFLSFMAFSITSIIWVIYGYPLAFGTDVMGIIGSPANILMNGIDVNALAPLAPTIPLLVYVAFQMTFAAITVALVSGAIVERMKFSAWLAFVPLWITIVYLPISHWVWGGGWLSQLGVLDFAGGTVVHLNAGIAALALVLLLGKRKDIRLLPHNLGYSVIGAALLWFGWFGFNAGSALSAGGLAGSAFLATNTATAAALISWVMMDVIKTGKPTLLGAISGAIAGLVAITPAAGFVTMGGALVIGFLVSIFCYIAISTVKSKFGYDDALDVFGIHGVGGAWGALATGIFAAPFVNSLGTGALYGNPGQIVTQLIGIAVVGVYTLIMTLIIGVVIDKTIGLRVDLKEEVEGLDTHLHEESGYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA44</accession>
    <name>F0TA44_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645345.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1771"/>
    <dbReference type="EnsemblBacteria" id="ADZ09994">
        <property type="protein sequence ID" value="ADZ09994"/>
        <property type="gene ID" value="Metbo_1771"/>
    </dbReference>
    <dbReference type="GeneID" id="10278228"/>
    <dbReference type="KEGG" id="mel:Metbo_1771"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="EEHEVKI"/>
    <dbReference type="OrthoDB" id="125987at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8068" checksum="B0EDB71CB0CD55AA" modified="2011-05-03" version="1">MFGNNYGNERENNAPIEEGSEYDVKIEDTGRDGDGIARVEGFVVFVAGAKVGDEVRIRVNSVRRNFGFADIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA72</accession>
    <name>F0TA72_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645373.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1800"/>
    <dbReference type="EnsemblBacteria" id="ADZ10022">
        <property type="protein sequence ID" value="ADZ10022"/>
        <property type="gene ID" value="Metbo_1800"/>
    </dbReference>
    <dbReference type="GeneID" id="10278257"/>
    <dbReference type="KEGG" id="mel:Metbo_1800"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="GAMSENF"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="18"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="296"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="393" mass="41184" checksum="65BA7AC54F7DDE92" modified="2011-05-03" version="1" precursor="true">MKFRNLILKNIFRNKSRSLLAIIGIAIGVAAVVGLGMVTDNLSKSTENALTAGAGDFSVIAATGPNNGGGPPGGMGGDQVINQTKVTEISKITGVGSAVGVLRTSVDLSNSTNTTTNSTSGPSNFRSMTSIIGIDSSSISMDDIVVTNGTVYSNNNEVIIGKTAAQEMNKTVGDNISISNNTFKITGIYETGNFMDDRGIVMSLDSLQSLTNKTGEVSLIIVKANNGTDATSLATKIQNNYPNELTTSTSLSGMDRMNNGLDVINSGSWAVSLLAMLVGGIIVIITMLKAVAERTREIGVLRAVGWTQKRIIAMIMGESIVLSFIAIFVGLVIGVGIVELLSTTNILRGIMPAFSIFLFLKGIGVALLLGILGGIYPAYRASRLSPTEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA97</accession>
    <name>F0TA97_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645398.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1825"/>
    <dbReference type="EnsemblBacteria" id="ADZ10047">
        <property type="protein sequence ID" value="ADZ10047"/>
        <property type="gene ID" value="Metbo_1825"/>
    </dbReference>
    <dbReference type="GeneID" id="10278282"/>
    <dbReference type="KEGG" id="mel:Metbo_1825"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="NWNQMRQ"/>
    <dbReference type="OrthoDB" id="67630at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="25215" checksum="568E3AAD87D74629" modified="2011-05-03" version="1">MNNTENVIEIKNLKKSYDNGNIKALNGIDLTVKKGEFISIMGPSGSGKSTLLNMIGALDTADEGMIKVAGIDLMKAKKLNLFRSQEIGFVFQMHNLIPNLSVIENVEIPMYETKANSSEMRERALELLKSVNLEKKIDQKPTKLSGGERQRVAIARALVNHPSIILADEPTGSLDSKTGEVILDLLKELHTKENVTLVMVTHEPYVGNMAERLVTVLDGKCKTDEKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC5</accession>
    <name>F0TAC5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0626</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644228.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0626"/>
    <dbReference type="EnsemblBacteria" id="ADZ08877">
        <property type="protein sequence ID" value="ADZ08877"/>
        <property type="gene ID" value="Metbo_0626"/>
    </dbReference>
    <dbReference type="GeneID" id="10277071"/>
    <dbReference type="KEGG" id="mel:Metbo_0626"/>
    <dbReference type="eggNOG" id="arCOG01091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111441_1_0_2"/>
    <dbReference type="OMA" id="RETMICG"/>
    <dbReference type="OrthoDB" id="115855at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006682">
        <property type="entry name" value="CHP00267"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00267">
        <property type="entry name" value="TIGR00267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="192" mass="20316" checksum="24D0489BAD3F5AB9" modified="2011-05-03" version="1">MSIREFIQEYLKMSRYVALGTLDGILAVMGVTLAASGVASAGGLPIPNYVIGLTGLSGGIALALSNAFGSFIGERAEEARNLRELEQKMVIGEGALDDTVIHKQAKKRVYMSMFVHGFSSFIGSFVPVLPFILIATRTTAIFTTIGLCFIALVILGIYLGKVSRESLLKTSVEIVLIGVLIGVVSFVIGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD2</accession>
    <name>F0TAD2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SNARE associated Golgi protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0633"/>
    <dbReference type="EnsemblBacteria" id="ADZ08884">
        <property type="protein sequence ID" value="ADZ08884"/>
        <property type="gene ID" value="Metbo_0633"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0633"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_2_2"/>
    <dbReference type="OMA" id="PWYGIGR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="23198" checksum="819BB6F7DFB27BF9" modified="2011-05-03" version="1">MIGILENLSNLAINLINSLGYWGVFIAMTVESACIPLPSEITMPFSGFVVYQGTTNMTLIGITIVGALGNLFGSLIAYYVGLIGGRPLLEKYGKYVFITHSKLELADKWFEKYGYEAVFISRLLPIIRTFISLPAGITHMDLKKFVTYTFLGSLPWCFALAYLGLILGPHWEIIKTYFHILDYFVVLGIVIVVGYLVYKYKFKSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAD7</accession>
    <name>F0TAD7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644240.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0638"/>
    <dbReference type="EnsemblBacteria" id="ADZ08889">
        <property type="protein sequence ID" value="ADZ08889"/>
        <property type="gene ID" value="Metbo_0638"/>
    </dbReference>
    <dbReference type="GeneID" id="10277083"/>
    <dbReference type="KEGG" id="mel:Metbo_0638"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_690043_0_0_2"/>
    <dbReference type="OMA" id="TSITENY"/>
    <dbReference type="OrthoDB" id="91115at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="2">
        <location>
            <begin position="10"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="408" mass="45724" checksum="30A80C6C77DBC586" modified="2011-05-03" version="1">MKNSDLLFKLERIVIDLVKVDGVDGGLIVDDTGKVVAKRMLQDIDIELFGPMSNVITSSSKRLFNSSHQGEIQRVLIESLNGKALFLNLENVHLIVLMKNHSNVGLILVSSKRAAEKIRVLSKDLKPDIVETIIEPAKEPLKTEIEPEVSPEKLEVVKSEVAVDEISDLKLVETPQEEPVVLEESLELEPQIIETESEDKSIQVLESLEVKEFEIETEIAEDVETSKPEAEEEVEEEVESEEVIPIIKPPIAFPDIKKITEVPEDVEARSELILDIYEAVFRAMSIGASKIMGVAPARGLTQKFLPSQECERLLNGVVVESNSTLNFDKIRENASKIKVEDRESEFIEDFTKILDVITDNYGKVMGYEAFRGMVRPEFKIIADSYGPVMNKLGIINKMHPEISELFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF2</accession>
    <name>F0TAF2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0653</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644255.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0653"/>
    <dbReference type="EnsemblBacteria" id="ADZ08904">
        <property type="protein sequence ID" value="ADZ08904"/>
        <property type="gene ID" value="Metbo_0653"/>
    </dbReference>
    <dbReference type="GeneID" id="10277098"/>
    <dbReference type="KEGG" id="mel:Metbo_0653"/>
    <dbReference type="eggNOG" id="arCOG02828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762098_0_0_2"/>
    <dbReference type="OMA" id="TSQECGK"/>
    <dbReference type="OrthoDB" id="28941at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DUF1611-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DUF1611_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="UCP026760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="PTHR40690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1611_N" evidence="2">
        <location>
            <begin position="46"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1611" evidence="1">
        <location>
            <begin position="154"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="352" mass="38160" checksum="C5745E7A634D6F0B" modified="2011-05-03" version="1">MYFITSLQEIQDLNPFIIIGCGGGGEKFSNFEGVEAVGFIDDNIEKQGQKFCGHTVSGNLKELLETTDAKSVAIMLPIGAEGSALKYAVEAIDSGKNVVTSFRSLPLAQNESIFKFADSKGVVIKEISPRLDVIRKIFGTAPPMCTEVLPKLSYKHKTPVVFVGGTSQECGKRTTTRMLGQAAKEKGLNAVVISTDEMGFEQPVDMNFRAGSLSVMDVASAVIGSIKYMEELKQPDIIFVEGQSSITEMGNPHPRGLSTAILIGAMPDATVVCHRPNHPYREPRGIDYEVRAIEAVEPTSVVGLSVNLRNVKDKSELAKFEEKYGLPAVDVRNGGASRLLDVIIDYIGDLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG6</accession>
    <name>F0TAG6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Indolepyruvate oxidoreductase subunit IorB</fullName>
            <ecNumber evidence="3">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Indolepyruvate ferredoxin oxidoreductase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0667</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>CoA + indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] = (indol-3-yl)acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644269.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0667"/>
    <dbReference type="EnsemblBacteria" id="ADZ08918">
        <property type="protein sequence ID" value="ADZ08918"/>
        <property type="gene ID" value="Metbo_0667"/>
    </dbReference>
    <dbReference type="GeneID" id="10277112"/>
    <dbReference type="KEGG" id="mel:Metbo_0667"/>
    <dbReference type="eggNOG" id="arCOG01602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_1_1_2"/>
    <dbReference type="OMA" id="HGMSQRF"/>
    <dbReference type="OrthoDB" id="120779at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017719">
        <property type="entry name" value="Indolepyruvate_Fd_OxRdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03334">
        <property type="entry name" value="IOR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="8">
        <location>
            <begin position="12"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="20988" checksum="67B0531521FFA462" modified="2011-05-03" version="1">MKSYSIYISGVGGQGIIKTSTVMGEAAMKTGLPVVMSEVHGMAQRGGSVSTELKIGDNYSPIIELGSADLLISFEPVEALRAVPKISKGSYIILNTNPIYPFNLNETGVSYPDMEDVLTELNGKAKQVYALDADTIARESGHSLSMNMVMLGAASAVEGFPIEKETIIDSMKANLPEKSLEINLKAFNAGFSFVKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH2</accession>
    <name>F0TAH2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644275.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08924">
        <property type="protein sequence ID" value="ADZ08924"/>
        <property type="gene ID" value="Metbo_0673"/>
    </dbReference>
    <dbReference type="GeneID" id="10277118"/>
    <dbReference type="KEGG" id="mel:Metbo_0673"/>
    <dbReference type="eggNOG" id="arCOG06478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133049_0_0_2"/>
    <dbReference type="OMA" id="FSGIVEM"/>
    <dbReference type="OrthoDB" id="128504at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005325">
        <property type="entry name" value="DUF308_memb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03729">
        <property type="entry name" value="DUF308"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="16653" checksum="BCE957295C3CFC61" modified="2011-05-03" version="1" precursor="true">MQKLGSAIVLIILGIIVMAFPLIGVVPAAVLTGFLVLVLGLGLLFSGIVEMGDSVGLGILEVLLGIIALVLGIGFIFNPALFSFVAGLIVYIVGIFLIIVGIIGVITKAGDSRWNGVVALIIGLLYVIVGTLFASNPVYLGILIGLWLLIMGIMMLFSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ9</accession>
    <name>F0TAJ9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized NAD(FAD)-dependent dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645422.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1849"/>
    <dbReference type="EnsemblBacteria" id="ADZ10071">
        <property type="protein sequence ID" value="ADZ10071"/>
        <property type="gene ID" value="Metbo_1849"/>
    </dbReference>
    <dbReference type="GeneID" id="10278306"/>
    <dbReference type="KEGG" id="mel:Metbo_1849"/>
    <dbReference type="eggNOG" id="arCOG02064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_1_1_2"/>
    <dbReference type="OMA" id="LQACQMT"/>
    <dbReference type="OrthoDB" id="100378at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="16058" checksum="A69BCF89CD9BC7B8" modified="2011-05-03" version="1">MVDEASKVDKSTIIVHSGDMDKIYSALIVSNGALSMGMEASLFFTFWGLQRLQKGGLDKGPLSKMHFLGLGKWMIKGRMKKANVASLERLIKDFKELGGKVIACEMTMEIMGISKEDLDQDLITEYGAVGTYVQEARNSKITLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL3</accession>
    <name>F0TAL3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylisocitrate lyase</fullName>
            <ecNumber evidence="1">4.1.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.3.30" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645436.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1864"/>
    <dbReference type="EnsemblBacteria" id="ADZ10085">
        <property type="protein sequence ID" value="ADZ10085"/>
        <property type="gene ID" value="Metbo_1864"/>
    </dbReference>
    <dbReference type="GeneID" id="10278322"/>
    <dbReference type="KEGG" id="mel:Metbo_1864"/>
    <dbReference type="eggNOG" id="arCOG00581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_2"/>
    <dbReference type="OMA" id="VKDLAPW"/>
    <dbReference type="OrthoDB" id="53531at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30607" checksum="F0979DFC33F3207A" modified="2011-05-03" version="1">MKTKILKNLLNSPETLVMPDAYDPISAKLIENAGFKAVQCSGYSFSIAAGYNNEIDVTLDENLEWTRRIVESVDVPVMADAEDGYGGPEVVMETVKKFLQTGVGGLNLEDQIPSRKNGISVVDADLMTEKIRVAKETAEIEAKPDFIINGRTDALRSTEDRYEGLEIAIDRANEYIEAGADLTFITYVATLDEVKQIKKEVKGPVTIAAGQPYNINEFTINDLKTYNVERVSLPTLLIFTSLYGINLALEHLKKDSLSEMFEEELVYPADGLNKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP4</accession>
    <name>F0TAP4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1896"/>
    <dbReference type="EnsemblBacteria" id="ADZ10116">
        <property type="protein sequence ID" value="ADZ10116"/>
        <property type="gene ID" value="Metbo_1896"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1896"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="WELDINT"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="232"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="499" mass="57170" checksum="349A5549B9252687" modified="2011-05-03" version="1">MRFQACDVEKNSSEKIKMDEQIKILIIEDVPFDAELIDRELQRSGMNFISRRVEEEHEYLHELEHFMPDIILADHSLPNFDGLSALKIANQLVPDVPFIFVSGKMGEDFAIEALKCGATDYVLKGSLSKIVHAVNRALEEVIEHSKRKMAEEALINSHRQLLEAQKIGHIGSWELDINTNKMRCSAEFYNIMGTDPSKLGDGYNAIIELIHPEDRPMVEKSILNAIELHESFSHDYRILKSDGSVRILSSKGLVINETSGAAPRLVVTEQDITEQKVAEEKIRSSLKEKEMLLAEIHHRVKNNLQVISSLLRLQSRFIEDDASIEIFKETQNRVRSIAILHEKLYQSEDLAKIKIDEYVKLLAEDLLYFYELENSKVEMNIDVEEVYLNIETAIPCGLIIDELVSNSLKYAFKPGENGTITIKLHSNENDRFTLQVSDDGVGIPLTVDPENSETFGMQLIKYLSNQLKAEMELDRRDGTKFKLEFNELQYKNRVFNEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ5</accession>
    <name>F0TAZ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645492.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1921"/>
    <dbReference type="EnsemblBacteria" id="ADZ10141">
        <property type="protein sequence ID" value="ADZ10141"/>
        <property type="gene ID" value="Metbo_1921"/>
    </dbReference>
    <dbReference type="GeneID" id="10278379"/>
    <dbReference type="KEGG" id="mel:Metbo_1921"/>
    <dbReference type="eggNOG" id="arCOG02449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068049_1_0_2"/>
    <dbReference type="OrthoDB" id="73968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="29050" checksum="CEB4A4E49DC85CCE" modified="2011-05-03" version="1">MNHCIDFYFFSGTGNTLLVVKRMSEVFQSKGHEVHLKRIEKSMPDDVNTECTIGLGFPVAVLSTYDLVWKFIKELPDVDGTEIFMIDTLGGYSGGIVGPLKSILESKGYKTIGACEIVMPLNIFYIQDSTVNTKKVEEGLLKAENYALSLLNNESKWGHFPLLPEVMRFISKMGIRLTATSVHQKILKFKVDKLRCDKCGICRDICPSNNIKMGEYPLIGNSCEYCMRCVSMCPFNALVSIFNYQGKTYSAVKPKEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ8</accession>
    <name>F0TAZ8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645495.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1924"/>
    <dbReference type="EnsemblBacteria" id="ADZ10144">
        <property type="protein sequence ID" value="ADZ10144"/>
        <property type="gene ID" value="Metbo_1924"/>
    </dbReference>
    <dbReference type="GeneID" id="10278382"/>
    <dbReference type="KEGG" id="mel:Metbo_1924"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184949_1_0_2"/>
    <dbReference type="OMA" id="KNAMEIG"/>
    <dbReference type="OrthoDB" id="117276at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8677" checksum="D732C47CA99AB88E" modified="2011-05-03" version="1">MNVSEFLGTRVFDKNGFEIGKISDMLINPKEGVINFISISTGEFGIRRKDVQIKPSDVKVMGDYLLLNLAREDLDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAZ9</accession>
    <name>F0TAZ9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1925</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645496.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1925"/>
    <dbReference type="EnsemblBacteria" id="ADZ10145">
        <property type="protein sequence ID" value="ADZ10145"/>
        <property type="gene ID" value="Metbo_1925"/>
    </dbReference>
    <dbReference type="GeneID" id="10278383"/>
    <dbReference type="KEGG" id="mel:Metbo_1925"/>
    <dbReference type="eggNOG" id="arCOG00607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037123_1_1_2"/>
    <dbReference type="OMA" id="IELHITF"/>
    <dbReference type="OrthoDB" id="51079at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="294"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006404-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="39843" checksum="82F6CE51D585A31D" modified="2011-05-03" version="1">MKYSFKIFSIFGIPVELHISFLVLIVLIYIIALLNVVSYVTLLTAVLITLIFVSVVLHELAHCYVAKRYGIGIERIVLLPIGGISEMEEIPKDPKKELRIALAGPVSNLLIGGICYLILILSLAYISKTIQAAIYYFVFVNILLGFFNLLPAFPMDGGRILRAFLAERMNFIDATKLAANIGKQLAIIMALVGIFFNFLLILVAVYVYLGAQAEYQTVLLSTLLENEKVKDVMTTTVHTVKPSNTVKETLKIMFKEKHMGYPVTDDGHLIGIVTFHDLSKIPEAEKDTLINDIMTKDLVVSDPEESLMETLEKLNRNRIGRVPIVWENRLVGIVSKTDITTVLDMKQNLLEKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB06</accession>
    <name>F0TB06_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1932</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645503.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1932"/>
    <dbReference type="EnsemblBacteria" id="ADZ10152">
        <property type="protein sequence ID" value="ADZ10152"/>
        <property type="gene ID" value="Metbo_1932"/>
    </dbReference>
    <dbReference type="GeneID" id="10278390"/>
    <dbReference type="KEGG" id="mel:Metbo_1932"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="OMA" id="APLYRIM"/>
    <dbReference type="OrthoDB" id="127272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="6">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7211" checksum="94ADEFD3BFF88256" modified="2011-05-03" version="1">MAELPIAPVGRIIKNAGAQRISDDAKEALAKSLEEKGEEIAKKAVELAKHAGRKTVKADDIDMAVKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB10</accession>
    <name>F0TB10_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation protein YtfJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645507.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1936"/>
    <dbReference type="EnsemblBacteria" id="ADZ10156">
        <property type="protein sequence ID" value="ADZ10156"/>
        <property type="gene ID" value="Metbo_1936"/>
    </dbReference>
    <dbReference type="GeneID" id="10278394"/>
    <dbReference type="KEGG" id="mel:Metbo_1936"/>
    <dbReference type="eggNOG" id="arCOG04972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115880_2_1_2"/>
    <dbReference type="OMA" id="EINGCTI"/>
    <dbReference type="OrthoDB" id="130975at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014229">
        <property type="entry name" value="Spore_YtfJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39162">
        <property type="entry name" value="PTHR39162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09579">
        <property type="entry name" value="Spore_YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021377">
        <property type="entry name" value="YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="151" mass="15838" checksum="37252BAA045FD971" modified="2011-05-03" version="1">MDINDPIKTTVEEIRKVLNIENVIGETIETDEFLLLPVTRMGMGFGAGEAEGKGMDNMGGAGAGAGGAAGVEPIAMVVVHKGVKGPEGVKVMSLKSPDPLSRAIGEISTAAVEIMDQGSKMMKEKGKAKAHFKGKDKGKDTKEAEIEVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB64</accession>
    <name>F0TB64_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA carboxylase, biotin carboxylase</fullName>
            <ecNumber evidence="7">6.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0764</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644366.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0764"/>
    <dbReference type="EnsemblBacteria" id="ADZ09015">
        <property type="protein sequence ID" value="ADZ09015"/>
        <property type="gene ID" value="Metbo_0764"/>
    </dbReference>
    <dbReference type="GeneID" id="10277212"/>
    <dbReference type="KEGG" id="mel:Metbo_0764"/>
    <dbReference type="eggNOG" id="arCOG01590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_2"/>
    <dbReference type="OMA" id="FVEICSH"/>
    <dbReference type="OrthoDB" id="36803at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="6">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="120"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="495" mass="54879" checksum="926D12B8BE407C38" modified="2011-05-03" version="1">MFKKVLIANRGEIAIRVMRACREMEIDSVAVYSDADKNSLFAKYADEAYNIGEPSPAKSYLDIDKIIDVAIKSGAEAIHPGYGFLAENSLLGEECEKHGIKLVGPSGRVIEEMGSKITSKKLMKRAGVPVVPGGSKAIDNVEEAAKMAEIVGYPVIIKASAGGGGIGMRTVFEEDELVRAIESTKSVAELNFGDSTVFVEKYIEEPRHIEFQILADEHGNTIHVADRECSIQRRHQKLIEESPSPIMTDELRAEMGEAAIKAAKSIGYTNAGTVEFIYSNGEFYFLEMNTRIQVEHPITEAVTGVDLVKEQLKIAAGRELCCEQNEISVRGHAIECRINAEDPLNDFAPNPGKIIGYRSPGGNGVRVDSGVYNNYTIPPYYDSMISKLIVWGRTRKEAIARMQRALSEYIILGVKTTIPFHKSMMVNQHFRDASLHTHFVDQYKNEIIENMEKVIAKDRDMEARLKSTFLPSRKVAAVSAAVSNYMSESIKKTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB75</accession>
    <name>F0TB75_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl4</name>
        <name evidence="6" type="ordered locus">Metbo_0775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644377.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0775"/>
    <dbReference type="EnsemblBacteria" id="ADZ09026">
        <property type="protein sequence ID" value="ADZ09026"/>
        <property type="gene ID" value="Metbo_0775"/>
    </dbReference>
    <dbReference type="GeneID" id="10277223"/>
    <dbReference type="KEGG" id="mel:Metbo_0775"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OMA" id="WHQKVNV"/>
    <dbReference type="OrthoDB" id="65833at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_L4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_L4/L1e_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_L4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_L4-archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="PTHR19431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="260" mass="28562" checksum="00C86BECD1E8569B" modified="2011-05-03" version="1">MEKIKVYSLEGEVVDEIELPEIFTEVYRPDLIKRAVISSQTARIQPWGTDPMAGKRTTAKSFGSGRGAAMVPRVKGSRHPAGSKAAFIPQAIGGRKAHPPKTERIIHEKINRKERRLAIRSAVAATANREIVEARGHKIQNLDQVPFVVDDELETVKRTKDTREIFAKLGLLDDVCRAKAGRTIRAGRGKMRGRKYKTSKGPLLVVGEDKGISLGARNHAGVDVVVVNHLNAELLAPGTHPGRLTIYTKSAVEKLGELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA7</accession>
    <name>F0TBA7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps11</name>
        <name evidence="7" type="ordered locus">Metbo_0807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644409.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0807"/>
    <dbReference type="EnsemblBacteria" id="ADZ09058">
        <property type="protein sequence ID" value="ADZ09058"/>
        <property type="gene ID" value="Metbo_0807"/>
    </dbReference>
    <dbReference type="GeneID" id="10277256"/>
    <dbReference type="KEGG" id="mel:Metbo_0807"/>
    <dbReference type="eggNOG" id="arCOG04240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="114285at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_S11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="13699" checksum="BEB55AB0384C1276" modified="2011-05-03" version="1">MAEKEKWGVANVYSSFNNTIITVTDITGAETITQWSGGKVVRADRQESSPFAAMEAATRAAEDVKEKGIIGLHIKVRAPGGNGPRTPGPGAQATIRALARAGIRIGKIEDVTPIPHDGTGRPGGKRGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE7</accession>
    <name>F0TBE7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_1997"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="55" mass="5930" checksum="CE89DDF04CE68E3A" modified="2011-05-03" version="1">MTELEMLIAILAPAVAIILFLLGLTFQVLQRITKVETNVSLLLQGVACLNGNMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF6</accession>
    <name>F0TBF6_METLA</name>
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        <submittedName>
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        </submittedName>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
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        <property type="protein sequence ID" value="ADZ10225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ10225">
        <property type="protein sequence ID" value="ADZ10225"/>
        <property type="gene ID" value="Metbo_2006"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="76604at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="2">
        <location>
            <begin position="9"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="189" mass="20746" checksum="0B35B48C7C4718B6" modified="2011-05-03" version="1">MKKSLGAKTIVYPTPVFIVGTYDENEEPDVMAAAWGGISCSNPPCVSISLQKHRYTLKNINERKAFTISIPSEGYVSEADYFGIVSGKDENKFEKTGLTPVKSELVDAPYVEEFPLVLECKAINTLDLGMHVQITGEILDVKLDESAATDTGKPDIEKIKPFTYDPAAISYRAIGKNLGKAFNLGMKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM4</accession>
    <name>F0TBM4_METLA</name>
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        <submittedName>
            <fullName evidence="10">Xenobiotic-transporting ATPase</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0851</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.44" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0851"/>
    <dbReference type="EnsemblBacteria" id="ADZ09101">
        <property type="protein sequence ID" value="ADZ09101"/>
        <property type="gene ID" value="Metbo_0851"/>
    </dbReference>
    <dbReference type="GeneID" id="10277300"/>
    <dbReference type="KEGG" id="mel:Metbo_0851"/>
    <dbReference type="eggNOG" id="arCOG02841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_2"/>
    <dbReference type="OMA" id="FSYLWFM"/>
    <dbReference type="OrthoDB" id="7900at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
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        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="180"/>
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        <location>
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            <end position="203"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="319"/>
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    </feature>
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        <location>
            <begin position="30"/>
            <end position="328"/>
        </location>
    </feature>
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            <end position="597"/>
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        <location>
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        <ligand>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="604" mass="68411" checksum="FFB82E9577DE10A3" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ0</accession>
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        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
    <gene>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        </reaction>
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</entry>
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    <accession>F0TBR3</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Land M."/>
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                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
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            <begin position="585"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="725" mass="82989" checksum="9C6A97E12911C4E2" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS0</accession>
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        <submittedName>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09147.1"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_2-like"/>
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    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="4"/>
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        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="ADZ09147.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="293" mass="34315" checksum="C4539DE86F59285B" modified="2011-05-03" version="1">MDLSIIIVNFETFEMTKQTVESVLNQSHPFDYDIYVVDNASSDGSFESLQKTFFKESENGKIKFIQNPENKGFAQANNVAIKRANSDYVLLLNSDTQVVGDCLERSMHYMKNHPDTGVLGCKVLLPDGNLDKACRRSFPEFSVSFYRMTGLSKLFPKSERFGRYNLTYLDEDDTYEVDCVMGAFMLLRSSTIEKVGLLDEKFFMYGEDIDWCYRIKAAGWKIIYYSEAQIIHYKGSSNSKKQNKRMIHEFYRAMYIFYNKHYREDYPMITTLATYMGIWSICQFKLFKNYITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW2</accession>
    <name>F0TBW2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645655.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2085"/>
    <dbReference type="EnsemblBacteria" id="ADZ10304">
        <property type="protein sequence ID" value="ADZ10304"/>
        <property type="gene ID" value="Metbo_2085"/>
    </dbReference>
    <dbReference type="GeneID" id="10278547"/>
    <dbReference type="KEGG" id="mel:Metbo_2085"/>
    <dbReference type="eggNOG" id="arCOG01371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_2"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="2">
        <location>
            <begin position="4"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="312" mass="35179" checksum="41025D669FB455F3" modified="2011-05-03" version="1">MKMLITGGAGFIGCNFVHQMVEKYDHDLVVFDKLTYAANPKYLDDVKDKIEFVKGDIGDAEAVKNVMKDCDYVVNFAAETHVDRSIEDPGVFVKTDVIGTYNLLENVRKYDVERYLQISTDEVYGSIENGSFTELSNIDPSSPYSASKAGADVLVSAYYKTYGAPVLITRSSNNFGPYQFPEKLIPLFILNAMQDKQLPVYGDGKNVRDWIYAPDNCRGVYTALMKGKLGEVYNVGGGNEKNNLEITKLILENLGKSEDLITFVEDRLGHDRRYSLDSTKIKKLGWKPEVKFEDAIKATIDWYKENISIFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW6</accession>
    <name>F0TBW6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10308">
        <property type="protein sequence ID" value="ADZ10308"/>
        <property type="gene ID" value="Metbo_2089"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2089"/>
    <dbReference type="HOGENOM" id="CLU_1006897_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30075" checksum="88E5849E9EA7672B" modified="2011-05-03" version="1">MAFLFAVILSGAASATESVHFSSDGKNITVTHNYVKSTLTTHVTTKKGYGNYGYGQYVTLTTSGKDNEGRNTIQTLYSFNGKPKQMIYKLWDSDSNAKVVANFYNTRAAYTMTGKDSATGATISGTGVSLYRTIAGQKYVGNTTMNMIYTINGKVLARITQITTYQYGAFNGGFQTVKTICTTKSAYAIANFNKKSVINSYYTRNSVGTLTGMKISGTSKGTETINNKLVSFTGKITISPKYDPKNIYTEGFSEGDYKEVLTSSSPTLLKFYPIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBY1</accession>
    <name>F0TBY1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645674.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2104"/>
    <dbReference type="EnsemblBacteria" id="ADZ10323">
        <property type="protein sequence ID" value="ADZ10323"/>
        <property type="gene ID" value="Metbo_2104"/>
    </dbReference>
    <dbReference type="GeneID" id="10278567"/>
    <dbReference type="KEGG" id="mel:Metbo_2104"/>
    <dbReference type="eggNOG" id="arCOG00554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010611_0_0_2"/>
    <dbReference type="OMA" id="SRRRCHE"/>
    <dbReference type="OrthoDB" id="3149at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="228"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="422"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="833" mass="94423" checksum="757BADA35657D0BE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC25</accession>
    <name>F0TC25_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644527.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09176">
        <property type="protein sequence ID" value="ADZ09176"/>
        <property type="gene ID" value="Metbo_0929"/>
    </dbReference>
    <dbReference type="GeneID" id="10277378"/>
    <dbReference type="KEGG" id="mel:Metbo_0929"/>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705173_0_0_2"/>
    <dbReference type="OrthoDB" id="85787at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="391" mass="42379" checksum="751039A0F7AAD5FE" modified="2011-05-03" version="1" precursor="true">MINKKMVSVLIGLLMIVSIGASAATSTTGNYSVSQIGQSSGNVKNYVETQNSLPSKVSVGNKNVTTAQFLYLMTSATSNLAKNNKNSIYFKNVSNPTSPSETFKGGTIDKNTYLSMASSVNSYIAKYGKAPNYVTTAQGTIKYQSMVYMYSKIMNYYKINNKLPTSVTLKSWYAQTLGPAATVNATSGFFKTSAVLGSTSYGKVLRLSPIGTGTNKVAIIIGVHPLEVQTHIAMLNAIYAMSKSLKNVQITIFDVIVYNGDNYDTGRSQGQTLASKYVVPNIGTSYKLVMDVHGNTGRGEEVYSGYPNFVFAPLQDSKSNSYASKIASSQYTTGLINHYVKGTSPIYVTIPIAKKGIPTIVYEQYVNQANYAKVMYLHAVEVLKSINSIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC85</accession>
    <name>F0TC85_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiX</name>
        <name evidence="7" type="ordered locus">Metbo_0989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644587.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0989"/>
    <dbReference type="EnsemblBacteria" id="ADZ09236">
        <property type="protein sequence ID" value="ADZ09236"/>
        <property type="gene ID" value="Metbo_0989"/>
    </dbReference>
    <dbReference type="GeneID" id="10277438"/>
    <dbReference type="KEGG" id="mel:Metbo_0989"/>
    <dbReference type="eggNOG" id="arCOG01703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074522_0_1_2"/>
    <dbReference type="OMA" id="GATHIQD"/>
    <dbReference type="OrthoDB" id="112962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="2"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="20649" checksum="1E5388D0522C8C53" modified="2011-05-03" version="1">MIVVAITGASGVIYGKRLLEVLKEKNIETAVVLTDPAKIILEYELNSTEEEIIDLATEFYPSKDLTTAINSGSFKFESMVIVPCTMKTLSAIANGYANNAVTRAADVALKERRKLVVVPRETPLRTVHLENMVQISREGGIILPAMPGFYHLPEKTEDLTDFVVGKILDVLGIENHLFQRWTGEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA0</accession>
    <name>F0TCA0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645718.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2152"/>
    <dbReference type="EnsemblBacteria" id="ADZ10367">
        <property type="protein sequence ID" value="ADZ10367"/>
        <property type="gene ID" value="Metbo_2152"/>
    </dbReference>
    <dbReference type="GeneID" id="10278615"/>
    <dbReference type="KEGG" id="mel:Metbo_2152"/>
    <dbReference type="eggNOG" id="arCOG14015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075001_0_0_2"/>
    <dbReference type="OMA" id="CSAFVNY"/>
    <dbReference type="OrthoDB" id="125221at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="31981" checksum="CF1F9F9807936BE5" modified="2011-05-03" version="1">MTLFSERNGYKSVQDTLKVECMDIDLRTSLWNVILIHVLDGTEPDDDYYQCYDEIKTLITKLFRVYWKRSLDELTEYTFFPFRAIKSYFFDCKWCEVYDFLEILVKIYPFPNVEEFTNECNDVLEMEHSGYRFVDISIMEITSEEEIIEIEEALNDAPNPVKIHLQKSLGLLSDRESPDYSNSIKESISAVESVCKQITGESLSLGQSLNKIEKNGIIDLHPSLKNGFKSIYGYTSDSDGIRHGLTDEPNLSYEDAKFMLVACSAFVNYLNAKWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB1</accession>
    <name>F0TCB1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArdcN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645729.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2163"/>
    <dbReference type="EnsemblBacteria" id="ADZ10378">
        <property type="protein sequence ID" value="ADZ10378"/>
        <property type="gene ID" value="Metbo_2163"/>
    </dbReference>
    <dbReference type="GeneID" id="10278626"/>
    <dbReference type="KEGG" id="mel:Metbo_2163"/>
    <dbReference type="eggNOG" id="arCOG07546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012069_0_2_2"/>
    <dbReference type="OMA" id="FKQWQTA"/>
    <dbReference type="OrthoDB" id="69558at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013610">
        <property type="entry name" value="ArdC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08401">
        <property type="entry name" value="ArdcN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArdcN" evidence="1">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="30401" checksum="BBF7CE7C8B62B0F9" modified="2011-05-03" version="1">MVFKIPKELKGKELTAFLVEQRDLLADSMVQDPEQLEKFVQMWEGNLGLHEYSFNNIILAWLQHGQVSMLAGFRKWQSLGRTVIKGEKAIKILAPLTRKIKDKESEEDDYVITGWRYVNVFDVSQTEGEELEFGHSDKVVGNVCFDDLKKISPLPVIVEYAGTSNGNVTKDRVLVSPKENQAAMVATLIHEMGHYKLGHLESDFDKETKEVEAETVSFIVTSYLGLKNEKSKYYVGSWNGSGEKLRGRGKNIIAVAEAIIKDINSLSGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD4</accession>
    <name>F0TCD4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645752.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2187"/>
    <dbReference type="EnsemblBacteria" id="ADZ10401">
        <property type="protein sequence ID" value="ADZ10401"/>
        <property type="gene ID" value="Metbo_2187"/>
    </dbReference>
    <dbReference type="GeneID" id="10278650"/>
    <dbReference type="KEGG" id="mel:Metbo_2187"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OMA" id="AVNPQMD"/>
    <dbReference type="OrthoDB" id="59861at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="PTHR10927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="SSF109728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="SSF89895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBDS" evidence="3">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SBDS_C" evidence="4">
        <location>
            <begin position="102"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="26179" checksum="79A14711F2627FE5" modified="2011-05-03" version="1">MVNLDDAVTARLEYFGEHFEILVDPDLASDFKRGQDINVEDILVVEEIFKDAKKGDKSSEEAMMKAFQTTDHVEVAANILKKGQIQLTAQQRKEMQEEKRLKVVTTIAREAINPQTKLPHPVRRIEIAMDEAKVRIDPFKSVDEQVNTTLKAIRVKIPIRFEKVKVAIRVPGDFSGKVYGAIPEFGKTMQEEWQQDGSWVAVVEIPGGMQDNFYQKLNELTHGQVETKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE6</accession>
    <name>F0TCE6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006600</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645764.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2199"/>
    <dbReference type="EnsemblBacteria" id="ADZ10413">
        <property type="protein sequence ID" value="ADZ10413"/>
        <property type="gene ID" value="Metbo_2199"/>
    </dbReference>
    <dbReference type="GeneID" id="10278662"/>
    <dbReference type="KEGG" id="mel:Metbo_2199"/>
    <dbReference type="eggNOG" id="arCOG04845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142041_0_0_2"/>
    <dbReference type="OMA" id="HNDAAVN"/>
    <dbReference type="OrthoDB" id="96590at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036608">
        <property type="entry name" value="MTH777-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012033">
        <property type="entry name" value="UCP006600"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09001">
        <property type="entry name" value="DUF1890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006600">
        <property type="entry name" value="UCP006600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75181">
        <property type="entry name" value="SSF75181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17306" checksum="40FA9EB50D73507E" modified="2011-05-03" version="1">MKKALILLGCPEAPSQTPLAVYTTYKLKEIGYEVTVASTPSAMKLLEVSDPEGFYVTNKVDIESCLENLEEKEYDLLFGFVHKDAAAAYFVTFYHILNTKSVALVFEKDIDLLEKFIKDVQDSTDSEIVSARAYHNPTPLRVKVDRYIKSLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE9</accession>
    <name>F0TCE9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypA</name>
        <name evidence="5" type="ordered locus">Metbo_2202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645767.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2202"/>
    <dbReference type="EnsemblBacteria" id="ADZ10416">
        <property type="protein sequence ID" value="ADZ10416"/>
        <property type="gene ID" value="Metbo_2202"/>
    </dbReference>
    <dbReference type="GeneID" id="10278666"/>
    <dbReference type="KEGG" id="mel:Metbo_2202"/>
    <dbReference type="eggNOG" id="arCOG04426">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126929_2_0_2"/>
    <dbReference type="OMA" id="ILLCPCG"/>
    <dbReference type="OrthoDB" id="109617at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="PTHR34535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00100">
        <property type="entry name" value="hypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0533">Nickel</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14127" checksum="065A84EFA048997A" modified="2011-05-03" version="1">MHELSMADAMVKTVLDVAEKNDATEVLDVTIEIGKLTMLNPEQLKFLLDVLVENTLLEGAEINIVEVPVEINCNVCEYSGAADMDDSDHYLAIVECPECGERNVEITAGRECNVKNIKIEKEEGDEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH6</accession>
    <name>F0TCH6_METLA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Metbo_1006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_1006"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3194509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="45" mass="5402" checksum="1E522C6B121E4178" modified="2011-05-03" version="1">MKTEKEIRRMIEILEKTKIPSEDDKNKVKIQIDILKWVLGEPVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ1</accession>
    <name>F0TCJ1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADZ09268"/>
        <property type="gene ID" value="Metbo_1021"/>
    </dbReference>
    <dbReference type="GeneID" id="10277470"/>
    <dbReference type="KEGG" id="mel:Metbo_1021"/>
    <dbReference type="eggNOG" id="arCOG02036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OMA" id="IRTDLCD"/>
    <dbReference type="OrthoDB" id="7531at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
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        <property type="entry name" value="RACo_C"/>
    </dbReference>
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        <property type="entry name" value="Raco_middle"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rama_corrin_act"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rama_corrin_act"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="475"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="506"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="540" mass="59080" checksum="739AF6222658C882" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ9</accession>
    <name>F0TCJ9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ferrous iron transport protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1029</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644627.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1029"/>
    <dbReference type="EnsemblBacteria" id="ADZ09276">
        <property type="protein sequence ID" value="ADZ09276"/>
        <property type="gene ID" value="Metbo_1029"/>
    </dbReference>
    <dbReference type="GeneID" id="10277478"/>
    <dbReference type="KEGG" id="mel:Metbo_1029"/>
    <dbReference type="eggNOG" id="arCOG00359">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_2"/>
    <dbReference type="OMA" id="RWVAIKL"/>
    <dbReference type="OrthoDB" id="27688at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="593"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="648"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="11">
        <location>
            <begin position="32"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="670" mass="73577" checksum="112221DD3D33460C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM9</accession>
    <name>F0TCM9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphonate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1059</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644657.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1059"/>
    <dbReference type="EnsemblBacteria" id="ADZ09306">
        <property type="protein sequence ID" value="ADZ09306"/>
        <property type="gene ID" value="Metbo_1059"/>
    </dbReference>
    <dbReference type="GeneID" id="10277508"/>
    <dbReference type="KEGG" id="mel:Metbo_1059"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="DFTAFEF"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="306" mass="34251" checksum="E0905FE94F055C36" modified="2011-05-03" version="1">MIDAENLTRKFDDLIAVDNLSFHINEGEVFGFLGPNGAGKTTTIRMLSCLISKTSGEARIAGYDTSDESESLKIRKLIGLLPENVGLYDDLSAYKNLDFYGKLYDCSLSKRKENIEHFLKMLGLWDKRDVSAGTFSKGMKQKLAIARTLIHDPEILFLDEPTANLDPESSKTVREFILDLKNENKTIFINTHNLDEAQRICDKIGIFNKKLMAIGSPEDLERSVWSNKTVIQLKRVTEKILESLESLSIGKFTSNDTEIKIDVNDPELDNPLIVSTIVAAGGEVQYVNRLSPTLEEAYLKIVKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN2</accession>
    <name>F0TCN2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644660.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09309">
        <property type="protein sequence ID" value="ADZ09309"/>
        <property type="gene ID" value="Metbo_1063"/>
    </dbReference>
    <dbReference type="GeneID" id="10277512"/>
    <dbReference type="KEGG" id="mel:Metbo_1063"/>
    <dbReference type="eggNOG" id="arCOG10883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG10990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243028_0_0_2"/>
    <dbReference type="OMA" id="NTHYNRE"/>
    <dbReference type="OrthoDB" id="95407at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="25295" checksum="61FC3FF04E22CD65" modified="2011-05-03" version="1">MGYLVCSKCKSYYKLQSNESPKNFSDNCDCGGKLRYIENLDIVDPSWKQITFRKKPTMKERWNKKSKSLFSLPKINLKNRLNQFYYNYIGRHIYKARHQQRVHRNPPGMQAGFLNSLMNEFHLENIQWNLIIPMTIAITLILAFTHGISLLLTFVLLVIMGYLSKNMIIGAKNAVIAGAISFFLGSLFIGSFLLIIPYAILGIINGAVCGLIGGYLKTKGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP2</accession>
    <name>F0TCP2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645783.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2218"/>
    <dbReference type="EnsemblBacteria" id="ADZ10432">
        <property type="protein sequence ID" value="ADZ10432"/>
        <property type="gene ID" value="Metbo_2218"/>
    </dbReference>
    <dbReference type="GeneID" id="10278684"/>
    <dbReference type="KEGG" id="mel:Metbo_2218"/>
    <dbReference type="eggNOG" id="arCOG06474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773225_0_0_2"/>
    <dbReference type="OMA" id="RPGSYIK"/>
    <dbReference type="OrthoDB" id="90867at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="15763" checksum="297DFA4F8CC95F6E" modified="2011-05-03" version="1" precursor="true">MFIKIRRDTLIILLLAFLLIFSGRVMTYMSYASSTTSDQGVPISGVIVKGNDIVPLDSIKNSMANVGFRSGSYIQGDTLVTSKRQVPLNEAIDNAKQAVMLTTIPGTQITPIVAADVEVDNSTGIVTVNVIEDFSNIYIKGLNGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT0</accession>
    <name>F0TCT0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker 16 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645821.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2256"/>
    <dbReference type="EnsemblBacteria" id="ADZ10470">
        <property type="protein sequence ID" value="ADZ10470"/>
        <property type="gene ID" value="Metbo_2256"/>
    </dbReference>
    <dbReference type="GeneID" id="10278722"/>
    <dbReference type="KEGG" id="mel:Metbo_2256"/>
    <dbReference type="eggNOG" id="arCOG00621">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061500_0_0_2"/>
    <dbReference type="OMA" id="GCGEINP"/>
    <dbReference type="OrthoDB" id="22946at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017677">
        <property type="entry name" value="Methan_mark_16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03287">
        <property type="entry name" value="methan_mark_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="423" mass="45899" checksum="50B9872EC412CCFC" modified="2011-05-03" version="1">MKIRSVEEINKKIKAGNANVLSAEEISNLIKEGETPKAEDVDVVTTGTCGIMSGTAAVLHVPVSDPGAFKKASKISLNGVPGFPGPCPNEWLGSVDLVIYGTSHSSEDEKYGGGFLFKDIVAGNDIEVEVESIDGKTFHKTVTVDDIGTAQMLGTRMAFKNYNSFTNPSEELISSIFHAVDMEGPFKGLSVSGCGQLNPLQNDPVMKTICAGSKILLNGAESIIIGNGTRSSPEKPNLMITGDMKQMDPHFLGGFQTGAGPEVFDSVACAIPVLDNEIFEKTFILNKDIVLPITDIRGRHSVLSSTNYGEVWDNSDERPVYHPEKCIKCDNCVVRERCPTGAYSDNLNLKKCFGCGMCVHSCPNQAFTMETGSVSFGIEDKTYDMPIICRQSDIKRARELTAELRRRIEDGSFILNNCLDKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T3</accession>
    <name>F0T5T3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="7">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="7">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pheS</name>
        <name evidence="9" type="ordered locus">Metbo_1081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1081"/>
    <dbReference type="EnsemblBacteria" id="ADZ09326">
        <property type="protein sequence ID" value="ADZ09326"/>
        <property type="gene ID" value="Metbo_1081"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1081"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="261"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="522" mass="59533" checksum="AF1FFDD8CD6EF31D" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W9</accession>
    <name>F0T5W9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644713.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1117"/>
    <dbReference type="EnsemblBacteria" id="ADZ09362">
        <property type="protein sequence ID" value="ADZ09362"/>
        <property type="gene ID" value="Metbo_1117"/>
    </dbReference>
    <dbReference type="GeneID" id="10277567"/>
    <dbReference type="KEGG" id="mel:Metbo_1117"/>
    <dbReference type="eggNOG" id="arCOG03110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024970_3_0_2"/>
    <dbReference type="OMA" id="EREDWTQ"/>
    <dbReference type="OrthoDB" id="30404at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025831">
        <property type="entry name" value="RecG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038475">
        <property type="entry name" value="RecG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007421">
        <property type="entry name" value="Schlafen_AlbA_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038461">
        <property type="entry name" value="Schlafen_AlbA_2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04326">
        <property type="entry name" value="AlbA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13749">
        <property type="entry name" value="HATPase_c_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlbA_2" evidence="1">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c_4" evidence="2">
        <location>
            <begin position="292"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="452" mass="51709" checksum="A548F9EF6232A25F" modified="2011-05-03" version="1">MINLSKMIENGESEKIEFKNSLSLHNPIYTAISAFSNKNGGSILVGINDNKEVVGVSLGKNTLENLANEIQRNTDPHIFPTITVLKKNNNNVVIIEVEENEEKPVFFRDKAFIRVGKTNQKLSSSEIKRMILEGHSGVYWDEKICENALKDDIDVDKLKWFVNETNKQRNRDLDPELSIEETLSKLDLVKDGKLTNAAVLLFGKDPQRFVRQAEIQCGRFKGTKAIDFVDRKTFPGNIIDQRTKAIEFVKDHINLKTTIVGTERIDEWEYPIAAIRESITNCICHRNYKLSSNIQIRIFNDRLEFWGCGPLPEHLTVEDIKKPHNSHPRNPLIAKRFFDIKFIEHWGTGIERIIQSCLKAGLPKPIFEIKAGDFVVILNKYKITDEILRELNKRQQKAIDYLFLENRITNKEYRSINIGISRSTALNDLKELVDKGIIALEGSGPGAHYILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T603</accession>
    <name>F0T603_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaerobic ribonucleoside-triphosphate reductase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2296"/>
    <dbReference type="EnsemblBacteria" id="ADZ10510">
        <property type="protein sequence ID" value="ADZ10510"/>
        <property type="gene ID" value="Metbo_2296"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2296"/>
    <dbReference type="eggNOG" id="arCOG00952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078147_2_1_2"/>
    <dbReference type="OMA" id="MDIKAPL"/>
    <dbReference type="OrthoDB" id="101349at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012840">
        <property type="entry name" value="NrdG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01094">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02495">
        <property type="entry name" value="NrdG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="22"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="247" mass="27911" checksum="BCE49F4CBCA8A9D7" modified="2011-05-03" version="1">MSKKGSCSSSIEIGGMVISSIEFSGKMALVIFTAKCMLRCPYCHNSEIIEGGDVYQLEEIFQKIEESIDFIDSVVISGGEPLMQDTQVFEILKHAKSLELSTKMDTNGYYPEKLQKMIKLIDYVALDIKAPFNKYEQIIGEAIGSQVRSSMEICSEDPDTFLECRTTYVPALMEPEDVVEIAKSIQCDIYTLQQFNSKTVLDERLKEAVMPLRDDVKKIAESLKPYLDNIRIKTYEFGEEIISSSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T606</accession>
    <name>F0T606_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645864.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2299"/>
    <dbReference type="EnsemblBacteria" id="ADZ10513">
        <property type="protein sequence ID" value="ADZ10513"/>
        <property type="gene ID" value="Metbo_2299"/>
    </dbReference>
    <dbReference type="GeneID" id="10278765"/>
    <dbReference type="KEGG" id="mel:Metbo_2299"/>
    <dbReference type="eggNOG" id="arCOG11926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039096_1_0_2"/>
    <dbReference type="OMA" id="AFGVVNF"/>
    <dbReference type="OrthoDB" id="41796at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="44009" checksum="6222439F180229E3" modified="2011-05-03" version="1">METTKLTRITDLMTNFAVNTGLDPEFSSPVRYLWTDAFAVCNYMGLYNLTGDTHYLDLGTKLINQVHQVLGKLRDKSGWISGLDKKTGEKHPTIGGLRIGKKMDEREQNQEYDEILEWEMDGQYYHYITKWIHALNITAKVSGDTKYLQWAGELLKTAHDAFTYSPAPNIPSRLYWKMSVDLKRPLVSSMGQQDPMDGYVTYKETETISSMFDGQRYEREINEISTICKKMDLKTNDPLGIGGLLTDATRTTQLMVYGSIKRPEILENLLNSALLGIKSYKETGQLALNPEHRLAFRELGLSIGFKGLDLMERCIENNPEIFISTHLKQLKQLQNYRYMVRYIDEFWLDPKNQYSMNWKEHRDINTVMLATAMYPDGFLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T631</accession>
    <name>F0T631_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2325"/>
    <dbReference type="EnsemblBacteria" id="ADZ10538">
        <property type="protein sequence ID" value="ADZ10538"/>
        <property type="gene ID" value="Metbo_2325"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2325"/>
    <dbReference type="eggNOG" id="arCOG04456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129126_1_0_2"/>
    <dbReference type="OMA" id="FQGYGYS"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009702">
        <property type="entry name" value="DUF1284"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06935">
        <property type="entry name" value="DUF1284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15793" checksum="3B6E4FDB78EC5211" modified="2011-05-03" version="1">MLEGTDKALIKIRAHHLLCLQGFQGYGYNKNFALGMKEILRVLKSDPSPNIQLVTEADEICGICPNLVDGVCVDCIKIKTMDLNVIETISLENNQIITFKNALQIIDKELSLESIKKICEGCVWMDKCLFFLNKIGSKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T677</accession>
    <name>F0T677_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644743.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1148"/>
    <dbReference type="EnsemblBacteria" id="ADZ09392">
        <property type="protein sequence ID" value="ADZ09392"/>
        <property type="gene ID" value="Metbo_1148"/>
    </dbReference>
    <dbReference type="GeneID" id="10277598"/>
    <dbReference type="KEGG" id="mel:Metbo_1148"/>
    <dbReference type="eggNOG" id="arCOG01518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_0_2_2"/>
    <dbReference type="OMA" id="FGWPAIH"/>
    <dbReference type="OrthoDB" id="27966at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="341" mass="37267" checksum="F38228ABAC820ED5" modified="2011-05-03" version="1">MDSLLKKLSDAPGVSGFEEEIREIIIDELKDHVDEVEVDQMGNVITTRKGKPNGKKIMLAAHMDEIGLMIRYIDKNGFIKFSKIGGINDQMLLNQEVTIHTDDGEVTGVIGSKPPHRMKESERKKILDYENMFIDIGAKTKEEAEERVKLGDPITIKQEFTKLGNFYKGKALDNRIGCAIMIEVMKRAETDATLYGVGTVQEEVGLKGAKTSAFKLNPDMALALDVTIAGDHPGIKEEDAPAKMGKGPAVILVDASGRGLITHPMVKKLIITAGNEDVIPYQLEVSEGGTTDATAIHLTREGIPTGVISPPTRYIHTPVSVVDMEDVENAVNLILAVLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T692</accession>
    <name>F0T692_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein Metbo_1163</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="877455.Metbo_1163"/>
    <dbReference type="EnsemblBacteria" id="ADZ09407">
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        <property type="gene ID" value="Metbo_1163"/>
    </dbReference>
    <dbReference type="GeneID" id="10277613"/>
    <dbReference type="KEGG" id="mel:Metbo_1163"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="OMA" id="DYMIVNE"/>
    <dbReference type="OrthoDB" id="101213at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTP_hydrolase-rel"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="209" mass="23857" checksum="8B444ACA17207867" modified="2011-05-03" version="1">MKVIGVTGLPGSGKSVVSRVAKQLGVHVIRMGDVIREEAKNRNKTTGEVAVELRREFGEFVIADRCVSKILNYNQKPNTPNQRKNPVKVNKPTIFMIEGIRSDYEVEIFKKKFKDFKIIAVHSTPQTRFKRLKKRMRPDDSREESEFKLRDKRELDFGIGNVIATADYMVVNEGPLKKIKGIVRSILENELQSNGKGKGKSNRRTRQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E7</accession>
    <name>F0T6E7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09462">
        <property type="protein sequence ID" value="ADZ09462"/>
        <property type="gene ID" value="Metbo_1220"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1220"/>
    <dbReference type="eggNOG" id="arCOG00563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587455_0_0_2"/>
    <dbReference type="OMA" id="NTNQFLV"/>
    <dbReference type="OrthoDB" id="27401at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="535" mass="60347" checksum="C417F663434780A6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6E9</accession>
    <name>F0T6E9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645934.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2370"/>
    <dbReference type="EnsemblBacteria" id="ADZ10583">
        <property type="protein sequence ID" value="ADZ10583"/>
        <property type="gene ID" value="Metbo_2370"/>
    </dbReference>
    <dbReference type="GeneID" id="10278836"/>
    <dbReference type="KEGG" id="mel:Metbo_2370"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_0_2"/>
    <dbReference type="OMA" id="GKPLMHG"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="186"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="412" mass="44830" checksum="3E97015844DD9164" modified="2011-05-03" version="1">MGNEFLDIKNPDDVKGIISNFQTIKKYEAVSIENSLYRVLAEDIHATINLPPFRKVTMDGYAVISEDTFLASEDEPVKLGLLEVIGAGDVPKEIVKNGFCTEVGTGSPLPEGADSVVMVEYTEVRDGIISIHESVTMGQNIAKEGSDIGEGELLLKKGTTITPEKIGVLSAIGLKTVPVYKKPEIAVISTGNELIRYDEEIQYGKIFDINSQTISSAVTACGCIPVKTEIVKDNYNDLKRKIIECQDMDLIITSGGTSAGRGDVLRQVLDDLGEVLVHGIAVKPGKPTIIGRFENEGVDKYVVGLPGNPVAASMVFQLFFAPFLKDMASIKTKSRLRKTNTIKIPLSRRYRPAKGRKHFLLVKVDDDNAIPILKDSGAITSLAEADGYIEIGKNVEIVEKGTLVKVHPLGNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J5</accession>
    <name>F0T6J5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in the regulation of nitrogen fixation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645980.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2416"/>
    <dbReference type="EnsemblBacteria" id="ADZ10629">
        <property type="protein sequence ID" value="ADZ10629"/>
        <property type="gene ID" value="Metbo_2416"/>
    </dbReference>
    <dbReference type="GeneID" id="10278888"/>
    <dbReference type="KEGG" id="mel:Metbo_2416"/>
    <dbReference type="eggNOG" id="arCOG02308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_1_2"/>
    <dbReference type="OMA" id="KYIPKRM"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13523" checksum="ACA0462B55155C5C" modified="2011-05-03" version="1">MKEITAIIRPKKVTRTKEVLDSLGFPAMNASRVMGRGKQRAILDEVNFEIDDPALLEMEGAMKYIPKRMISLVVPDEEVALVVQAIIKVNHTGQIGDGKVFVSPIEEAFRVRTSEQGDNAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K2</accession>
    <name>F0T6K2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name evidence="3" type="ordered locus">Metbo_2423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645987.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2423"/>
    <dbReference type="EnsemblBacteria" id="ADZ10636">
        <property type="protein sequence ID" value="ADZ10636"/>
        <property type="gene ID" value="Metbo_2423"/>
    </dbReference>
    <dbReference type="GeneID" id="10278895"/>
    <dbReference type="KEGG" id="mel:Metbo_2423"/>
    <dbReference type="eggNOG" id="arCOG04705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="OMA" id="FHQFVGT"/>
    <dbReference type="OrthoDB" id="110834at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="SSF143560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHNA" evidence="2">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="122" mass="13890" checksum="DA73439958C0FB2E" modified="2011-05-03" version="1">MNAEEKYFNNITNRERAIFEGAITMGALFHQFVGTPVNIDSVESLEKAIEAAMSVQPCVFRVEVVIDRKKLEEISNEFDYVSLTGEMLDVKVTTVCSDKQALIRLKFVSELNYPLMYVEDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N6</accession>
    <name>F0T6N6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ureidoglycolate lyase</fullName>
            <ecNumber evidence="2">4.3.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643620.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0016"/>
    <dbReference type="EnsemblBacteria" id="ADZ08269">
        <property type="protein sequence ID" value="ADZ08269"/>
        <property type="gene ID" value="Metbo_0016"/>
    </dbReference>
    <dbReference type="GeneID" id="10276438"/>
    <dbReference type="KEGG" id="mel:Metbo_0016"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_2"/>
    <dbReference type="OMA" id="PPEPIIF"/>
    <dbReference type="OrthoDB" id="72644at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050385">
        <property type="term" value="F:ureidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="62"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="28896" checksum="63F33890A83FDB2C" modified="2011-05-03" version="1">MKLMGFESKGTEKVGIVSDEELIEIDCSMIQALNCQDINTIKRVKTHDISELEIKPPIRPSKIVCVGLNYQDHAKELDMELPTEPIIFIKPSTTVIGHLDPIIYPKTSTQVDYESELGIVISKEAHRVNRKNAQEFIGGFTVVNDVTARDKQRKDVQWTRAKSFDTFAPIGPCIETEVDPMNLNISLKLNNEIKQNSNTKNMIFNVNELVEFISSIMTLLPGDIISTGTPPGVGPMHIGDTVEIEIEGIGTLKNYLKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q7</accession>
    <name>F0T6Q7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker 13 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643641.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0037"/>
    <dbReference type="EnsemblBacteria" id="ADZ08290">
        <property type="protein sequence ID" value="ADZ08290"/>
        <property type="gene ID" value="Metbo_0037"/>
    </dbReference>
    <dbReference type="GeneID" id="10276459"/>
    <dbReference type="KEGG" id="mel:Metbo_0037"/>
    <dbReference type="eggNOG" id="arCOG04888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782144_0_0_2"/>
    <dbReference type="OMA" id="CASMIIG"/>
    <dbReference type="OrthoDB" id="56870at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017675">
        <property type="entry name" value="CfbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03282">
        <property type="entry name" value="methan_mark_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="1">
        <location>
            <begin position="10"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="359" mass="39086" checksum="417C733E1226FD0C" modified="2011-05-03" version="1">MHPRPSPIAASLYTLRDMNADVIVLHGPHGCCFRTGRLLENDGVRVVTTSMSENDFIFGASEKLEEVLLKVEELFNPKLVGVVGTCVSMIIGEDMKEAVKNAGINAQVVTVESHGGLGEGDNTEGAIAVLDAASNQGIISRDEMERQTKMLKKATEIEKTRGMAQGSYIKPSYGDNKVKVANIILNAFKSNKKIAFILNSKKETSYLFADILNIPFREYFPENEYTVIANLDENVGLPRIRGHASNITKELLESGTTVKHITGGLDEYPVTGIKAEQILNEGNFDLVVVLGVPHAFPVEKVNSVTVAVTDGPRLVEPLKSLGYDYVVTELDAHSKTLGTDKIVPSDLGDSLRGLMEIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V1</accession>
    <name>F0T6V1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1229</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644822.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1229"/>
    <dbReference type="EnsemblBacteria" id="ADZ09471">
        <property type="protein sequence ID" value="ADZ09471"/>
        <property type="gene ID" value="Metbo_1229"/>
    </dbReference>
    <dbReference type="GeneID" id="10277680"/>
    <dbReference type="KEGG" id="mel:Metbo_1229"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_6_0_2"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="126"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="236" mass="27156" checksum="363C5B7732B2F227" modified="2011-05-03" version="1">MKEREEPKTNKEIEIKTSDPEKSKEIKEQKSKKTSKLNIEAEENKKAAENYVDDLKQASSEKGQEIKEGLKDVKKEAKKKKEKLIEESKARNVHPTEKVLGDIISKFKQGTDQINEMMADYTKESKSKKSFKLPLVDVFDTNEEIILIADIPGVKKEHINLGISKNCVEIEVKYKDVPDIKDSKFIINERSYGIKKRKIPIKSDINVKKANAKWEDSVLTITLPKKERDLTKIDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Y2</accession>
    <name>F0T6Y2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="gene ID" value="Metbo_1260"/>
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    <dbReference type="eggNOG" id="arCOG09164">
        <property type="taxonomic scope" value="Archaea"/>
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        <source>
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    <sequence length="292" mass="32753" checksum="39F4010E79ED79DD" modified="2011-05-03" version="1">METFLDQANKGKTSIIRYVVGIFIIYFFIYIVGAVVQFLVAMAMGSTVELGRFNISSISPLAYFVVTMCPAICGIIGVFISVRFIHQRPFSTLVTPFKKINAKPILYGFGFFFLLLVLSDIILGGLNTSNIHFNANNISQWLYFLPFILILIPLQTTSEELTYRGYWMQGTGLLTRNFFILALINGILFLLPHIVNPEITAGGILAMVYYVVVGFSLAYITLKSGTLELAIGMHAANNIYESAIIHPQASILQTPSLFTQAIDPIYELLTIIVIMVVFYLVTFKIKPQWKRC</sequence>
</entry>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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        <property type="gene ID" value="Metbo_1275"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG02183">
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    <dbReference type="OrthoDB" id="93710at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
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        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fer4_7"/>
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    </dbReference>
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    </dbReference>
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    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS51379"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09517.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="342" mass="38742" checksum="F98E38213425C4DA" modified="2011-05-03" version="1">MSSVIWYLYEFARKSWAENFAAAKSDKEIMDAPDRFRNFPEVHKEYCIACGACTAACPAPMAIKLVRDEDSEHEDGFTYPVINNRGCIRCGFCAEVCPTDPKTLTCGENHLIREDFTILPVDKKFVIDDYLCIRCKKCMKTCKVGDAIFEEDNKIVIDQSKCISCGECLKTCPVKGAIKGIHISHVEEQKEIINLVVDGLEAKIEAEQNKIKNIEGSDVYSFDISAVELIEDARKILDDDELIDDIIENITDRLKLRVITWDKDKCTNCRLCVKECPSGAIKYTSEEGVVRNPDKCLRCSICYQTCPFGAIGYYISRFLYKRDEADKLIHVTVKTSDLPVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T713</accession>
    <name>F0T713_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1291"/>
    <dbReference type="EnsemblBacteria" id="ADZ09533">
        <property type="protein sequence ID" value="ADZ09533"/>
        <property type="gene ID" value="Metbo_1291"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OrthoDB" id="65668at2157"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018701">
        <property type="entry name" value="DUF2206_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09971">
        <property type="entry name" value="DUF2206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="35"/>
            <end position="52"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T767</accession>
    <name>F0T767_METLA</name>
    <protein>
        <submittedName>
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        </lineage>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.34" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2488"/>
    <dbReference type="EnsemblBacteria" id="ADZ10701">
        <property type="protein sequence ID" value="ADZ10701"/>
        <property type="gene ID" value="Metbo_2488"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2488"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="OMA" id="YPRITWI"/>
    <dbReference type="OrthoDB" id="68573at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="29567" checksum="FBB7A49FC828C068" modified="2011-05-03" version="1">MTILEVKNATFAYENSDNIFEGVNLTVDRGDVICILGSNGCGKTTLMKCMNKLHELKDGAVYINGENIKTMDQRTIARNIGYIPQGHVSTFAFSVFDVVLMGRTPHLDYLDSPGKKDRLITEEALKKFGIYHLKNKPYTSLSGGEQQLVFFARVIAQQPQILLLDEPTSHLDFGNQLKTLNIISKLSQEGIAIVMTSHYPDHAFISANKVALMKDQGFLGIDTPNNIITTENMERVYGIHVKIMEISPNRKICVPIEILTDYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T768</accession>
    <name>F0T768_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_2489</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2489"/>
    <dbReference type="EnsemblBacteria" id="ADZ10702">
        <property type="protein sequence ID" value="ADZ10702"/>
        <property type="gene ID" value="Metbo_2489"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2489"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_2"/>
    <dbReference type="OMA" id="MMSSWKL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="37727" checksum="AF167365E2483391" modified="2011-05-03" version="1">MSTEGNSVQRFFSDSEGKKWFLNFILITLPIILFFVSFMIGRYPLSPLEVVTTIIAKFIPSIHVSATAATIVWDIRLPRIMAAILVGAALAVAGAAFQGTFKNPLVSPDILGVSSGAGFGAAIAILLIGIPLIIQMSAFAWGLIAVSITYFVARSIRNSEILVMVLSGMAIASFFTALISLCKYTADPYSKLPQIVYWLMGSLASVTNAQVLMVSIPIIVGISILLILRWRLNVLAMGDEESKSLGVETNKLRFIIIVCCTLITAAAVSISGIIGWVGLIIPHISRMLVGPDHKRLLPATICLGATFLLLIDNISRTVLSIEVPIGILTAIIGAPFFLYLLRRGYNQWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T779</accession>
    <name>F0T779_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum-pterin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646064.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2500"/>
    <dbReference type="EnsemblBacteria" id="ADZ10713">
        <property type="protein sequence ID" value="ADZ10713"/>
        <property type="gene ID" value="Metbo_2500"/>
    </dbReference>
    <dbReference type="GeneID" id="10278974"/>
    <dbReference type="KEGG" id="mel:Metbo_2500"/>
    <dbReference type="eggNOG" id="arCOG00228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118993_1_2_2"/>
    <dbReference type="OMA" id="KMSIRNQ"/>
    <dbReference type="OrthoDB" id="124036at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00638">
        <property type="entry name" value="Mop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mop" evidence="3">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="7461" checksum="1D236A99B543065B" modified="2011-05-03" version="1">MKISARNVFEGEVKNIEIGAIMSNVKIEVTSPEVITAIITKESVEKLGLKEGDKVSAIIKSTEVMVAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T780</accession>
    <name>F0T780_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646065.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2501"/>
    <dbReference type="EnsemblBacteria" id="ADZ10714">
        <property type="protein sequence ID" value="ADZ10714"/>
        <property type="gene ID" value="Metbo_2501"/>
    </dbReference>
    <dbReference type="GeneID" id="10278975"/>
    <dbReference type="KEGG" id="mel:Metbo_2501"/>
    <dbReference type="eggNOG" id="arCOG04074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_11_4_2"/>
    <dbReference type="OMA" id="DLCIQNC"/>
    <dbReference type="OrthoDB" id="119501at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="6753" checksum="13E7B2CBA9CE71EE" modified="2011-05-03" version="1">MPVTIDTEKCGKIQNCPGEGLCIKICEKGALVEEDGDVVLVPENCDDCDLCIQNCPNQAISKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T791</accession>
    <name>F0T791_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646076.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2512"/>
    <dbReference type="EnsemblBacteria" id="ADZ10725">
        <property type="protein sequence ID" value="ADZ10725"/>
        <property type="gene ID" value="Metbo_2512"/>
    </dbReference>
    <dbReference type="GeneID" id="10278986"/>
    <dbReference type="KEGG" id="mel:Metbo_2512"/>
    <dbReference type="eggNOG" id="arCOG00292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_4_2"/>
    <dbReference type="OMA" id="WGGKGPY"/>
    <dbReference type="OrthoDB" id="118147at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="76" mass="8099" checksum="7D64AA07CB0E9F75" modified="2011-05-03" version="1">MELKVNQDNCLGCGVCVVACPVNVSISPEVSGGHGSKTTETIMMVENGVIKLFSEDKCTKCGTCQVFCPTDAIWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7A2</accession>
    <name>F0T7A2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643682.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0078"/>
    <dbReference type="EnsemblBacteria" id="ADZ08331">
        <property type="protein sequence ID" value="ADZ08331"/>
        <property type="gene ID" value="Metbo_0078"/>
    </dbReference>
    <dbReference type="GeneID" id="10276501"/>
    <dbReference type="KEGG" id="mel:Metbo_0078"/>
    <dbReference type="eggNOG" id="arCOG00519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068890_3_1_2"/>
    <dbReference type="OMA" id="IVATPVW"/>
    <dbReference type="OrthoDB" id="63381at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12682">
        <property type="entry name" value="Flavodoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="19169" checksum="F81C42DC640F561B" modified="2011-05-03" version="1">MKTLIACYSYSGHTLKVAKKLQKEIGADLTEIKTEKDRWYLLKILDSIREKKATIKPCQTDLMNYDKLILCCPVWAGKTPAAVNQYMFDLKNIKDKEFGVFITSGGNRSQKATIQMRENLDLQGMKFLGQMRLITKDVENEKYGEIFPLFAGKFKDPKSENSKNITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7B0</accession>
    <name>F0T7B0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="11">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrS</name>
        <name evidence="14" type="ordered locus">Metbo_0086</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643690.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0086"/>
    <dbReference type="EnsemblBacteria" id="ADZ08339">
        <property type="protein sequence ID" value="ADZ08339"/>
        <property type="gene ID" value="Metbo_0086"/>
    </dbReference>
    <dbReference type="GeneID" id="10276509"/>
    <dbReference type="KEGG" id="mel:Metbo_0086"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029833_0_0_2"/>
    <dbReference type="OMA" id="IATFQID"/>
    <dbReference type="OrthoDB" id="11656at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="13">
        <location>
            <begin position="201"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ08339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="614" mass="70315" checksum="ED0C487180A3ED93" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C4</accession>
    <name>F0T7C4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein Metbo_0100</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643704.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0100"/>
    <dbReference type="EnsemblBacteria" id="ADZ08353">
        <property type="protein sequence ID" value="ADZ08353"/>
        <property type="gene ID" value="Metbo_0100"/>
    </dbReference>
    <dbReference type="GeneID" id="10276525"/>
    <dbReference type="KEGG" id="mel:Metbo_0100"/>
    <dbReference type="eggNOG" id="arCOG04272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="OMA" id="TRWVAED"/>
    <dbReference type="OrthoDB" id="41909at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="entry name" value="PTHR38811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00303">
        <property type="entry name" value="TIGR00303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="356" mass="37220" checksum="98FC8FDF5935C036" modified="2011-05-03" version="1">MDEAIKCYGSSDKIDNLKCKDSLFLCVIASTETSRIPGITGAGATPELTDYTPAADVELVILGNPICLPEIPKTVVNGGSAPTPAVITKAALDLSEIPFMVADAGAAIKPNVPYININAVPGGNIGTGRAILNSEEIFNKGFILGKTLSKLTDHLIIGESTPAGTTTALGVLTALGYDVDHKISGSTPVNPHDLKHKIVEDGLIASGYKPGELNDEPFKAIDSVGDPMIPAVAGIVMGGDISVTLAGGTQMTAVCAVIKAINPDYDFTDLSIATTIFVAEDESSDLIYLKRQIGDITVFAVDPYFEKSNTEGLINYTKGSVKEGVGAGGAMFAALLKGKSVDEIRARTEQLCREIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H1</accession>
    <name>F0T7H1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cadmium resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0148"/>
    <dbReference type="EnsemblBacteria" id="ADZ08400">
        <property type="protein sequence ID" value="ADZ08400"/>
        <property type="gene ID" value="Metbo_0148"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0148"/>
    <dbReference type="eggNOG" id="arCOG11200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071117_1_0_2"/>
    <dbReference type="OrthoDB" id="101109at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004676">
        <property type="entry name" value="Cd-R_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03596">
        <property type="entry name" value="Cad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="206" mass="23182" checksum="58BA954FB32E12C9" modified="2011-05-03" version="1">MEIMVVVLIAVGVFIATNLDDIFILMSFFAMSEDRIKLIFGQYLGFLILLCISLACYQFKFVLSMHYLAVLGLFPIFIGIKYFWNFRKNIFTDVMLEKNFGPTGTQIGDALKGGNTILTVAMTTVANGGDNLAVYIPLFITLDNFELSITLLTFMVMVGVWCVLAYKMVNNRIVGEKLKIYGHLIFPVVLIIIGLGIIFRSLPFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H4</accession>
    <name>F0T7H4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GHMP kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644893.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1300"/>
    <dbReference type="EnsemblBacteria" id="ADZ09542">
        <property type="protein sequence ID" value="ADZ09542"/>
        <property type="gene ID" value="Metbo_1300"/>
    </dbReference>
    <dbReference type="GeneID" id="10277751"/>
    <dbReference type="KEGG" id="mel:Metbo_1300"/>
    <dbReference type="eggNOG" id="arCOG01030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741049_0_0_2"/>
    <dbReference type="OrthoDB" id="52771at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="1">
        <location>
            <begin position="131"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="373" mass="41901" checksum="DDB063ADB256B0D2" modified="2011-05-03" version="1">MVQLYDYLTHAKEKNATPGVRAYNRIDGPGSSLDLRDFQQAFWKLPNMSLEYDPSCKWSSALPRTVGITIDTGTKIEAFPYNPEMICVKSTEYNKEVSGRSGEIIPVKDNWLLKILEIFNLQGVKMVLTNLRKDIHSSGLGGSSTATIGVCILANELAGRPFNDVQLVSLASRIEQDMGVSITGTQEQSNVMFGGVTDYVWFPWGIPGQKSSGYGGSLRFELMKEENYHNLQDRIAIFHSGHPRDSSNVNTVWEEALKTHKGYDLHSKKLAVAYKFREGLRLENWDQVQESIENYRMIRADLCADYMKGSMDIWHEAEDHDSVVFPLGAGGGGAVFIFSKYKDSMADLRDDIQGSFTEIPSKIRSKGHEFMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J5</accession>
    <name>F0T7J5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644914.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09563">
        <property type="protein sequence ID" value="ADZ09563"/>
        <property type="gene ID" value="Metbo_1321"/>
    </dbReference>
    <dbReference type="GeneID" id="24964549"/>
    <dbReference type="KEGG" id="mel:Metbo_1321"/>
    <dbReference type="HOGENOM" id="CLU_2629746_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8108" checksum="408B8D47E2E06022" modified="2011-05-03" version="1">MFKKVLLILLIIRGSLLIIESFINLGSVGVIFGSLYVEFVVAVILGVIIGIISSLLGVAGGEVIIPILILVFGIDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K0</accession>
    <name>F0T7K0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1327</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644919.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1327"/>
    <dbReference type="EnsemblBacteria" id="ADZ09568">
        <property type="protein sequence ID" value="ADZ09568"/>
        <property type="gene ID" value="Metbo_1327"/>
    </dbReference>
    <dbReference type="GeneID" id="10277778"/>
    <dbReference type="KEGG" id="mel:Metbo_1327"/>
    <dbReference type="eggNOG" id="arCOG04921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000892_0_2_2"/>
    <dbReference type="OMA" id="SHELKHP"/>
    <dbReference type="OrthoDB" id="72293at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheB-type methylesterase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="CheR-type methyltransferase" evidence="9">
        <location>
            <begin position="227"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="680"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="978" mass="110976" checksum="3D575331ACD758A1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M5</accession>
    <name>F0T7M5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644944.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1353"/>
    <dbReference type="EnsemblBacteria" id="ADZ09593">
        <property type="protein sequence ID" value="ADZ09593"/>
        <property type="gene ID" value="Metbo_1353"/>
    </dbReference>
    <dbReference type="GeneID" id="10277804"/>
    <dbReference type="KEGG" id="mel:Metbo_1353"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="OMA" id="DWQLTER"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="147"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="341" mass="38763" checksum="FF69290DA6F517E6" modified="2011-05-03" version="1">MEDFELNNEGLPTIPEIAWEFIFDSIEAPIMILDDKHRIVRINESMKQAFNIPDDPILEKCYKIVHGTENPPDFCPHSQTLKNNQQCTEEVELSDIDAWLLVTTSPIQNNDGEPIGSTHIAQDITKLKQTQEKNQQSLELKDLLIKETHHRVKNNLVTLSSLLYLQAEHVEDQKAKDILLDSKNRARAMAIIHQKLYSNKNLETINLSYYLNQLMDEVLKSYSITDKIKYILDLDNIILDSSTALIVGLVVNELVSNSLKYAFPDDALGVISVSFHENDGEYILKISDNGKTIPQDVDINNIDSFGLTIVNLLVNQIEGQISVERVAGTTFTIKFKEKREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q5</accession>
    <name>F0T7Q5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2524"/>
    <dbReference type="EnsemblBacteria" id="ADZ10737">
        <property type="protein sequence ID" value="ADZ10737"/>
        <property type="gene ID" value="Metbo_2524"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2524"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_2"/>
    <dbReference type="OMA" id="SQQIARM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="4"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="303" mass="33279" checksum="37537648CBDC9C64" modified="2011-05-03" version="1">MNKTDLLAIGHTALDYIIQVKEFPEANSSTSINKMKTLFGGAAANVAVVASTLGLKVSLVSAVGSGFIDSDYHNKLKDLRIDTQSMIVIEDENTPTAFVLTDNEKDQISYFYWGAASEFKNSEVPYNAIEKVEAVHLATGDPGFNSRSGEAAHDSGKLISFDPGQDLHMYSEAELKDVLKITDILFGNHHEIKRIQEILNMNIDDLRNFGPEIVVTTYGKDGSRINSGNEIRIDAVLKETVDPTGAGDSYRAGFLNAYLRGEDLEYCGKFASSVSSFIVEAEGCQTNVPDENMVKTRMNDMWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R9</accession>
    <name>F0T7R9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="7">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ileS</name>
        <name evidence="11" type="ordered locus">Metbo_0154</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643757.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0154"/>
    <dbReference type="EnsemblBacteria" id="ADZ08406">
        <property type="protein sequence ID" value="ADZ08406"/>
        <property type="gene ID" value="Metbo_0154"/>
    </dbReference>
    <dbReference type="GeneID" id="10276580"/>
    <dbReference type="KEGG" id="mel:Metbo_0154"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="10">
        <location>
            <begin position="695"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="609"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1060" mass="122723" checksum="3AE9561E0016C1B6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T8</accession>
    <name>F0T7T8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="8" type="ordered locus">Metbo_0173</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643776.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0173"/>
    <dbReference type="EnsemblBacteria" id="ADZ08425">
        <property type="protein sequence ID" value="ADZ08425"/>
        <property type="gene ID" value="Metbo_0173"/>
    </dbReference>
    <dbReference type="GeneID" id="10276599"/>
    <dbReference type="KEGG" id="mel:Metbo_0173"/>
    <dbReference type="eggNOG" id="arCOG01323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="OMA" id="SGPGMTR"/>
    <dbReference type="OrthoDB" id="88531at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="14907" checksum="6D070FEE838C246F" modified="2011-05-03" version="1">MVKVRIGAVVSEFNYDITQMMLELAKEHAIFLGSEITEVIAVPGVFDMPLAIKKLLKNDEIDAVVTMGAVIEGATSHDEIVVQHASRKIADLALEYDKPVALGISGPGMTRLEAHQRVEYGKRAVEAAVKMYKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T803</accession>
    <name>F0T803_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_1389</name>
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    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644980.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1389"/>
    <dbReference type="EnsemblBacteria" id="ADZ09629">
        <property type="protein sequence ID" value="ADZ09629"/>
        <property type="gene ID" value="Metbo_1389"/>
    </dbReference>
    <dbReference type="GeneID" id="10277840"/>
    <dbReference type="KEGG" id="mel:Metbo_1389"/>
    <dbReference type="eggNOG" id="arCOG04662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192627_0_0_2"/>
    <dbReference type="OMA" id="YGRIAYY"/>
    <dbReference type="OrthoDB" id="91006at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="234" mass="26377" checksum="3469F9A6C2AECECB" modified="2011-05-03" version="1">MSFLNPEEKDVNQFERKILIIMRVFLLIAGIYITIFGGINGSERIFGVLILFALLIINAPAFFTRHKIRAIPIEIELLILFMVLFELVCGDALGLYVKLPHYDNFMHFMLPLYVALIGMMAVYTMYYYGRLKASYWEMAILIVIITIGFGGMLEMGEYTYDKFLANGPVGEITGNTQMQGSPTQNALDDTMNDLFTDTLGGIVGAVLGVFFIRRAEKSGGHWKVADEIERMGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T808</accession>
    <name>F0T808_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1394"/>
    <dbReference type="EnsemblBacteria" id="ADZ09634">
        <property type="protein sequence ID" value="ADZ09634"/>
        <property type="gene ID" value="Metbo_1394"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1394"/>
    <dbReference type="HOGENOM" id="CLU_3282850_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="40" mass="4699" checksum="5C7348A6FCA14070" modified="2011-05-03" version="1">MSNNKIIFNMLFSSIILYNKYSMLLDTPIKYEIKGKILVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T823</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="6 7">
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            <taxon>Methanobacteriales</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ09649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1410"/>
    <dbReference type="EnsemblBacteria" id="ADZ09649">
        <property type="protein sequence ID" value="ADZ09649"/>
        <property type="gene ID" value="Metbo_1410"/>
    </dbReference>
    <dbReference type="GeneID" id="10277861"/>
    <dbReference type="KEGG" id="mel:Metbo_1410"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_5_2"/>
    <dbReference type="OMA" id="FSATQMF"/>
    <dbReference type="OrthoDB" id="118856at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="24"/>
        </location>
    </feature>
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        <location>
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            <end position="55"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="122"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="27213" checksum="2566BEB5553EDFFA" modified="2011-05-03" version="1">MIDLNLVLLPLFGLLIGLLVSMFGGGGGGLYVPVLILIFGVTPQVAIATSLASVLPTSVVSSISHFREGNVDIRTGLILGIGGVVGTLVGAVIANLIPPVLLEKIMGIFTLIMLIPMLRSLVQRQQKMKELNGENQEKTTLKGPKRAVASLFGVASGLLAGVFGISGTPPIIAGLYSLGLPALMVVGTSVFVLIFNSLAGIGGFYLLGRLNLTLIILLGGSAAVGAFIGPLLLKKINPNTFEKIYGPVIMGTMIIFALGLIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T824</accession>
    <name>F0T824_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645001.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1411"/>
    <dbReference type="EnsemblBacteria" id="ADZ09650">
        <property type="protein sequence ID" value="ADZ09650"/>
        <property type="gene ID" value="Metbo_1411"/>
    </dbReference>
    <dbReference type="GeneID" id="10277862"/>
    <dbReference type="KEGG" id="mel:Metbo_1411"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OMA" id="DPGHYGN"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="15"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="24160" checksum="30E8BF90B7A3CF78" modified="2011-05-03" version="1">MKIGENVYALESTKGSYSYLIMDKEPILIDTGYPGKFEDILEEINSLNLELKSIKHILLTHHDVDHVGNAALLQEETGATLWASKDDIPYILGDKCRPGIKKLVSFILRPKKPEKIQVYQNTTIGDVQIISTPGHTPGHVSFLYEDILFAGDLVGNTNGKLKKPPIFGNSNTKSIDESIIKMNEYDFNWICPAHGEPIELKDEWKQLLNNIRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T827</accession>
    <name>F0T827_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rieske (2Fe-2S) iron-sulfur domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1414</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645004.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1414"/>
    <dbReference type="EnsemblBacteria" id="ADZ09653">
        <property type="protein sequence ID" value="ADZ09653"/>
        <property type="gene ID" value="Metbo_1414"/>
    </dbReference>
    <dbReference type="GeneID" id="10277865"/>
    <dbReference type="KEGG" id="mel:Metbo_1414"/>
    <dbReference type="eggNOG" id="arCOG02852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_3_2"/>
    <dbReference type="OMA" id="HRSAFDL"/>
    <dbReference type="OrthoDB" id="102560at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="13027" checksum="D4F63364C9C08D8B" modified="2011-05-03" version="1">MVFVELLKKSELEDGGLKMVDIDGHEYLVARVGDEFMVSDNRCPHMGGDLSKGKLEGTVITCPKHHSQFDLVDGHVIRWTDWKGLKLTAAKIFKSPKDLKTYPIEIREDKIMADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T889</accession>
    <name>F0T889_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyco_trans_2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643852.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0249"/>
    <dbReference type="EnsemblBacteria" id="ADZ08501">
        <property type="protein sequence ID" value="ADZ08501"/>
        <property type="gene ID" value="Metbo_0249"/>
    </dbReference>
    <dbReference type="GeneID" id="10276681"/>
    <dbReference type="KEGG" id="mel:Metbo_0249"/>
    <dbReference type="eggNOG" id="arCOG01385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_467436_0_0_2"/>
    <dbReference type="OMA" id="CGEGMGH"/>
    <dbReference type="OrthoDB" id="15899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005262">
        <property type="entry name" value="CHP00661"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00661">
        <property type="entry name" value="MJ1255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="587" mass="67106" checksum="73257803C6415DA3" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A1</accession>
    <name>F0T8A1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Energy-converting hydrogenase B, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643864.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0261"/>
    <dbReference type="EnsemblBacteria" id="ADZ08513">
        <property type="protein sequence ID" value="ADZ08513"/>
        <property type="gene ID" value="Metbo_0261"/>
    </dbReference>
    <dbReference type="GeneID" id="10276693"/>
    <dbReference type="KEGG" id="mel:Metbo_0261"/>
    <dbReference type="eggNOG" id="arCOG04877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171540_0_0_2"/>
    <dbReference type="OMA" id="GVIDCAC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="9836" checksum="21F21AD5A15877B0" modified="2011-05-03" version="1">MTDIITLIQSAILIIAAFLVLLAAYGILKYKDDIENIIYARIHILGVADTALILALLAINEPLLAVAYFILVPFAAHAVANGYFYGEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8A5</accession>
    <name>F0T8A5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="3">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ08517">
        <property type="protein sequence ID" value="ADZ08517"/>
        <property type="gene ID" value="Metbo_0265"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0265"/>
    <dbReference type="eggNOG" id="arCOG01537">
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    </dbReference>
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    <dbReference type="OMA" id="YVAHHIT"/>
    <dbReference type="OrthoDB" id="28394at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ND/Mrp_mem"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
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        </location>
    </feature>
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        <location>
            <begin position="261"/>
            <end position="285"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
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        <location>
            <begin position="317"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="405"/>
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        <location>
            <begin position="425"/>
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            <begin position="143"/>
            <end position="434"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="500" mass="53787" checksum="69815D054A131F64" modified="2011-05-03" version="1">MSTENLLIPLMIIIPITCALFLNLLHEKSRVVKIISVIVALLLPTIPLLASYGIQYFGGYAPLAQNPTIATGLPSFITGSALNIFHPAITYVFGSAQKLMITILGFVALFAIFISIYEVKKPSGVYIYLILMGVASVMAMLLSDDIFNLYVFFEIAALTQVGIVLVSKIDHNYETALKYLILGSIASPMLLLGIALLLGVTGNVNITDIVYSIRTGLVDPHSPVLLMAGSLIIFGWLYAAGLPPFHTIKSAMYSKALPTGAAVLQAFSVFTFVAIGVIILRIFSYLHFAEIFILGISLIAMVLGITMAITQTDFKRLIAFLAVGELGYIGVGFGLGTAFGITAGLFQAVNEAVITALLFIGFGTIMYKTKETELKKLGGMLVETPKSAFLVLLAGLAMAGVPPFNAFQSKLMLVQSSLNSGLPEVAIIMVLLSIVTFMTFMRAFHTIYLRPKPAGLVIENENIPKVTIVAMVCLLIICLVFGLYPQLATNYLQGLAMGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C0</accession>
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    </gene>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0280"/>
    <dbReference type="EnsemblBacteria" id="ADZ08532">
        <property type="protein sequence ID" value="ADZ08532"/>
        <property type="gene ID" value="Metbo_0280"/>
    </dbReference>
    <dbReference type="GeneID" id="10276712"/>
    <dbReference type="KEGG" id="mel:Metbo_0280"/>
    <dbReference type="eggNOG" id="arCOG02648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="88919at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
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        <property type="entry name" value="HTHTETR"/>
    </dbReference>
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        <property type="entry name" value="SSF46689"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="222" mass="26252" checksum="0F3D96D69A7C7241" modified="2011-05-03" version="1">MKKENSPQETLTTKERIFEASVNLFSQKGFDAVSMREIGREVGIRESSIYNHYKNKDAILENIINHLIDELGAISFTDEEMDALISESPELFFETGSREFFNGMSNPKTEKIWRILSIEVFHNEKIKTFFVNELLESPINEWEKIFTKMMKKGIIKEYDPRVLAREYFSFALYLFFEYFILKYDTKYSSFTDMAYNKMIDHTNFILKTVKIESNDNSNHKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C5</accession>
    <name>F0T8C5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0285"/>
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        <property type="protein sequence ID" value="ADZ08537"/>
        <property type="gene ID" value="Metbo_0285"/>
    </dbReference>
    <dbReference type="GeneID" id="10276717"/>
    <dbReference type="KEGG" id="mel:Metbo_0285"/>
    <dbReference type="eggNOG" id="arCOG03004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141446_2_0_2"/>
    <dbReference type="OMA" id="MIIMPAN"/>
    <dbReference type="OrthoDB" id="105481at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
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    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
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        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="12032" checksum="E61AD1FA442D1CA1" modified="2011-05-03" version="1">MSEELKSKPLKVEDLIDYQENAVVSREIIRKETGTVTIFAFDKGEGLSEHTAPFDAMVQVVDGTAEIIISGEKNIVNKGELIIMPANEPHALNAIERYKMVLTMIRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E5</accession>
    <name>F0T8E5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide TPR_2 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="component" value="Chromosome"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="498" mass="58307" checksum="59C83B05EAC24938" modified="2011-05-03" version="1">MYENKGCCDELIIFINELLKSHPDNGLVWHKKGFYLDKMDKEEDAKNSFKKALECYNVEIEENPNKYAVWANKGVILFILEKYDEAMPCFERSLKLNQYYAYAWKHMGSCYGVMGKNDEALKCFDEAIKISPDDHEIWIDKGIALKNKNDYYKATACFNRALDFKAENTRAYYNKVFVFETLKEYEEALKVCDQIIKIDPSSYAWYLKGIIYGRMRRYREALESFDESLKLKPDDIILLNLKGNTFKYIKKYQEALDCFDNAIVLDPEDYNAYLNKSYVFEKLKDFKQALKNVDKAFELEPENNWVLGQKGYLLDEIGKTDESTVFLENALKYTLKEVEENPDEPYAWANKASDLVNLKRYQEALKAINKSLEFDPEYDWAIDFKGHILRKLQCYTDSMDYLDKAFNLDPDNSQNRYEKGRALCGLNQFDEALNLFNKSIELDPTNESAICAKGVIFKQTGKLDKSKEYFENCLEINPYFQPAKKNLNELKSMTEGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L9</accession>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ08561">
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        <property type="gene ID" value="Metbo_0309"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OMA" id="CFWVVQP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="AI-2E-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
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            <end position="185"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="377" mass="41813" checksum="E2C8801CF201A42B" modified="2011-05-03" version="1">MDYHILKQGYNMINGLKNNITSAIFIIILLMVVSAVLLTPMLSMVVLGAIFAYAIRPLSRKLEPYTKFQSVAIFVGMIIVILPLIIILLIFINTIISAAPSLIVFVKNLNLSSLNSTTLQNYPLIQQNFPGSSSSQMINSVVNSIYVGMGDVVRSVTEYLLGFVQSIPTLLLQLFIFFASTFYFARDGDRIWEYLNYIIPDDRKHYFKTLLKEIDLVLKSIFFGHFVTAALTGIISGIGFWIMGYPYPLFLGTLTGFFQLMPIIGHWPTIVALALYDLVIGNFLRAAAVISLGVLLSLMDMYVRPKVAGKYADIHPLIFLLGFICGPLVLGLVGFIIGPLVLGVTYAAVVAYKKENQDKNPEKLIDSKDAVKKDGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X9</accession>
    <name>F0T8X9_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1580</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645158.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09807">
        <property type="protein sequence ID" value="ADZ09807"/>
        <property type="gene ID" value="Metbo_1580"/>
    </dbReference>
    <dbReference type="GeneID" id="10278036"/>
    <dbReference type="KEGG" id="mel:Metbo_1580"/>
    <dbReference type="HOGENOM" id="CLU_2299359_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="11577" checksum="E1C512C1985967F3" modified="2011-05-03" version="1" precursor="true">MLKKLVLLLVLVMVIALNSQTIAAADYHKTYHLYDRFIIDTSAPFLCENSDFCIFNYKTEHVTYDQDHYLNYFVARDCGKAVVVDPGVHGNDYYYITVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y0</accession>
    <name>F0T8Y0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09808">
        <property type="protein sequence ID" value="ADZ09808"/>
        <property type="gene ID" value="Metbo_1581"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1581"/>
    <dbReference type="HOGENOM" id="CLU_693749_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="397" mass="45233" checksum="14BCD8EEC8E1E05E" modified="2011-05-03" version="1">MFIVVVTVQPLFGASESSHPTKITGADGKSLKDITVSAGSPIELQARLYWYFLPFTTDTFTWIPQTCRYLEFYIYKVNPDGTNGDLAWNDTAMTNFFTGNANPDEFKLTENGTYSLVVKYDGKLRHCNATAKIFVESQNNYKLWDKRITGPDREPLKDIHVRDGDLTQLQARISVYDHLCNENTSINKCWVDKIGLYLHFYIYKTNPDGSSGDLVWDDTAMTNLATFNANPDKFSLSEADTYNGTYNLVVKYEGDIARPCNATAKIFVEPRKNAVLGDTKIAGKYGCPLRDITVHSGSPIKLQARLYLYEDGMSMDWYNKPAWRPIPWYYLDFFVYKSNLDGTNGGLVWNDSAMTNYFTYNSNPDEFTLTEKGCYNLVVKYDGKMNHCRSDTKIFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z6</accession>
    <name>F0T8Z6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cl-channel voltage-gated family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1597"/>
    <dbReference type="EnsemblBacteria" id="ADZ09824">
        <property type="protein sequence ID" value="ADZ09824"/>
        <property type="gene ID" value="Metbo_1597"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1597"/>
    <dbReference type="eggNOG" id="arCOG02569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015263_8_0_2"/>
    <dbReference type="OMA" id="WKVPGHA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="42618" checksum="845599EF48C96D53" modified="2011-05-03" version="1">MGWGLLLGLIGGISALIFVVLMNFGQSFFVPHITQNWTPFSGPWWIVLLMTGVGVIIGLIHRYTPAKEMDVFGSFDKGYLDPKPMPSSVFASLISIIGGFSLGPEVSTGMLSGGLASWISKKRNLDPEITKTNYLSSVSGAWSGLFTSPLAMILILLETKHKQSVLYYGTLLIAGLAAVIGFSIFYASQGFNYSSLLGILSPPEYHLEIWHLGVAIIMGIIAVPVALLFVVFTKIFDRLIKPLDKKPVIRSTIGGLLLGLLVFALPVTLGLGTTSMLLVYQNAAEIGAVLLLIFAIAKMAALTGALRFGFIGGPIFPLLFTGSCMGALIHLIFPAVPVGLAMGCMIVAVPAAIVPIPFALAAIGIIIIGVSPQDALPIIIAALVAFSTTRGFILKGEEVETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T915</accession>
    <name>F0T915_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="6 10">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the aTrm56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="6 10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643988.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0385"/>
    <dbReference type="EnsemblBacteria" id="ADZ08637">
        <property type="protein sequence ID" value="ADZ08637"/>
        <property type="gene ID" value="Metbo_0385"/>
    </dbReference>
    <dbReference type="GeneID" id="10276822"/>
    <dbReference type="KEGG" id="mel:Metbo_0385"/>
    <dbReference type="eggNOG" id="arCOG01857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="OMA" id="VVHLTMY"/>
    <dbReference type="OrthoDB" id="83050at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="PTHR42197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="184" mass="20674" checksum="AFEF27A98070DFE7" modified="2011-05-03" version="1">MKVVVLRLDHRRVRDARITTHVCLTARAFGASKVVLSGDNDKKIIGNVRDVVERWGGDFEIDYCKNWSKYIDDWKSDGGEVVHLTMYGEAVQEITGTIAQSSKDKLVVVGGSRVPSKVYQDANWNVSVTNQPHSEVAALSIFLHTLYDGKEYDIDFEGSELKVVPNPHGKTVLTKERIIETEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T935</accession>
    <name>F0T935_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate/glutamate/uridylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0405"/>
    <dbReference type="EnsemblBacteria" id="ADZ08657">
        <property type="protein sequence ID" value="ADZ08657"/>
        <property type="gene ID" value="Metbo_0405"/>
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    <dbReference type="GeneID" id="10276842"/>
    <dbReference type="KEGG" id="mel:Metbo_0405"/>
    <dbReference type="eggNOG" id="arCOG00859">
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    <dbReference type="HOGENOM" id="CLU_089197_0_0_2"/>
    <dbReference type="OMA" id="LPHSWDV"/>
    <dbReference type="OrthoDB" id="85553at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="AceGlu_kinase-like_sf"/>
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        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="84"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="24170" checksum="BE30BF41AF268633" modified="2011-05-03" version="1">MDWVLKIGGSLFPEKAIDLCEFIVNQTDLDAEIIIVCGGGEFANKVRNYNDLLGFSNTANHRSAIMCMDIIGTLLADKVEGLESANSLEDALKVSEHGKIPVLNSYTLMEDHDPLEHSWRVTSDSIALYISNLLKAKLLIATDVDGIYTHDPINDGAKLIDTISANKLLNFGETSVDEFLPELLIRYQSECYVVNGKYPERILSIIKGKSSKYTLIGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T960</accession>
    <name>F0T960_METLA</name>
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        </submittedName>
    </protein>
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    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0430"/>
    <dbReference type="EnsemblBacteria" id="ADZ08682">
        <property type="protein sequence ID" value="ADZ08682"/>
        <property type="gene ID" value="Metbo_0430"/>
    </dbReference>
    <dbReference type="GeneID" id="10276867"/>
    <dbReference type="KEGG" id="mel:Metbo_0430"/>
    <dbReference type="eggNOG" id="arCOG04237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070533_2_0_2"/>
    <dbReference type="OMA" id="ETYIRGY"/>
    <dbReference type="OrthoDB" id="61183at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
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    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
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    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="30250" checksum="312EE5D73391B03A" modified="2011-05-03" version="1">MAIGRKTVENLLKLTNPKWKTSITRVEPNKLVTRGYSQEDLIGKISFPEMVYLLVNGELPSEIDSKMLEAVLVSFCDHGVTPPSTQVARLMASTGSSMKECVSGGLLSFGKFHAGAMERSMQMLQYLALNGVPEFRGPLKSGQDVKAVAEKVVDEYLSKNEKIPGYGHRYHSEDPRAAKLIQVAKKLECVGVHTQLALSIEEILYETKEIKMNIDGANAGILSDLGFDWKVGTGIFMMGRLPGIISHVFEEQTVETPFRKLIDTEEIHYSEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T991</accession>
    <name>F0T991_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645193.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1615"/>
    <dbReference type="EnsemblBacteria" id="ADZ09842">
        <property type="protein sequence ID" value="ADZ09842"/>
        <property type="gene ID" value="Metbo_1615"/>
    </dbReference>
    <dbReference type="GeneID" id="10278072"/>
    <dbReference type="KEGG" id="mel:Metbo_1615"/>
    <dbReference type="eggNOG" id="arCOG02497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_309879_0_0_2"/>
    <dbReference type="OMA" id="INCNQNI"/>
    <dbReference type="OrthoDB" id="7264at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
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        <property type="entry name" value="NosD_dom"/>
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        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="PbH1"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASH" evidence="2">
        <location>
            <begin position="57"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CASH" evidence="2">
        <location>
            <begin position="210"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="CASH" evidence="2">
        <location>
            <begin position="361"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="950" mass="100715" checksum="5E8B6D28348CEBB9" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A4</accession>
    <name>F0T9A4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645206.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09855">
        <property type="protein sequence ID" value="ADZ09855"/>
        <property type="gene ID" value="Metbo_1629"/>
    </dbReference>
    <dbReference type="GeneID" id="10278086"/>
    <dbReference type="KEGG" id="mel:Metbo_1629"/>
    <dbReference type="HOGENOM" id="CLU_1754713_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="16898" checksum="AB59808496AC5DA3" modified="2011-05-03" version="1" precursor="true">MKINKNIIFVLTLILGISLVLGTASAADSKTFVKTTVNKQTSVYAWEIFGYSYQNSYFKITKGTSYVNGYVTGINPMTGRSEKAPVYGYRVKITPLNSHVKIKKITIRSRGYPSGKLYSNTYYTCKNVFTPKKYMGFDRFTVYYTYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9B6</accession>
    <name>F0T9B6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1641</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645218.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1641"/>
    <dbReference type="EnsemblBacteria" id="ADZ09867">
        <property type="protein sequence ID" value="ADZ09867"/>
        <property type="gene ID" value="Metbo_1641"/>
    </dbReference>
    <dbReference type="GeneID" id="10278098"/>
    <dbReference type="KEGG" id="mel:Metbo_1641"/>
    <dbReference type="eggNOG" id="arCOG06459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130854_1_0_2"/>
    <dbReference type="OMA" id="FHLLCKL"/>
    <dbReference type="OrthoDB" id="102887at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1232" evidence="5">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16504" checksum="4143749ECE1902D3" modified="2011-05-03" version="1">MENQFKDFYDVLRENLDNYRGDYERFVDYGPDLYKLLSDLLNDQSLKSKYRLRICATLGYFVVPFDIIPEQIYGPHGYIDDIFLCSYVLNEVKEEIGIELLDEHWDGDEELENVLDVCYTSSKEILGDKTIEILNYVGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E9</accession>
    <name>F0T9E9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Activator of Hsp90 ATPase 1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645251.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1674"/>
    <dbReference type="EnsemblBacteria" id="ADZ09900">
        <property type="protein sequence ID" value="ADZ09900"/>
        <property type="gene ID" value="Metbo_1674"/>
    </dbReference>
    <dbReference type="GeneID" id="10278131"/>
    <dbReference type="KEGG" id="mel:Metbo_1674"/>
    <dbReference type="eggNOG" id="arCOG05261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_6_1_2"/>
    <dbReference type="OMA" id="LAQWWGR"/>
    <dbReference type="OrthoDB" id="87021at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="18322" checksum="D4289D88D7DD61DE" modified="2011-05-03" version="1">MGETEIVIEPGKQEILIKHIADASRELVFKAFTDPEIYAKWIGPVDMETELKLFEPKTGGSWKYIQRDEAGNEFGFHGVNHEVEPNERIIGTFEFEGLPEKGHVILESVKFVELEANKTEIQEQSVFLSVEDRDGMVGSNMEQGVRESYMQLDELLKSMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F1</accession>
    <name>F0T9F1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645253.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1676"/>
    <dbReference type="EnsemblBacteria" id="ADZ09902">
        <property type="protein sequence ID" value="ADZ09902"/>
        <property type="gene ID" value="Metbo_1676"/>
    </dbReference>
    <dbReference type="GeneID" id="24964570"/>
    <dbReference type="KEGG" id="mel:Metbo_1676"/>
    <dbReference type="eggNOG" id="arCOG02335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="124"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="484"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="578" mass="65652" checksum="E80E09FD94EA7017" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F5</accession>
    <name>F0T9F5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ08703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0451"/>
    <dbReference type="EnsemblBacteria" id="ADZ08703">
        <property type="protein sequence ID" value="ADZ08703"/>
        <property type="gene ID" value="Metbo_0451"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0451"/>
    <dbReference type="eggNOG" id="arCOG00238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OMA" id="LHGMNTY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002457">
        <property type="entry name" value="DASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="457" mass="48308" checksum="E761EE3563CEDE78" modified="2011-05-03" version="1">MSFKTLGMPIAIVAFIVVMLIPLGNGLSYSGHAAIGLLVFAVIMWASEAQHLAVTSLILLFLQPILGIESFNNAVIGFANPIIFLMIGGFILAEAIRKSGLAKRFTYFLLKKVGTTPSMSIFAAVFSTGILSAWIENVVAFAMLLPIIKEIVPLFGVNDAEKGNSNFAKAMVLGASYGSLAGGFGTEIGTAPNLMAAAYTHLPFANWMVFGFPLAIFMLFVTWKLLSWIFPPEVKGIVGGHETLNKTLSAMGSISRTEKITAVILFFTIGLWITTGITGLDSYSVALIGAALYFITGVVDWKDAQENVDWGLIIFFGGALSLGAALLNTGAAAWLINDIISLMGSNVSTVSIMLILMIIGVIFTQVMSNIALAAILVPLSVTLAAAQGQPVGIYAVPVAIACSLSFMFPMADPTVAMAYGTGYVKIKEILKAGIPMVVIGIIATVIVMLTIAKPFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G5</accession>
    <name>F0T9G5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Type II secretion system F domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0461</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644064.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0461"/>
    <dbReference type="EnsemblBacteria" id="ADZ08713">
        <property type="protein sequence ID" value="ADZ08713"/>
        <property type="gene ID" value="Metbo_0461"/>
    </dbReference>
    <dbReference type="GeneID" id="10276899"/>
    <dbReference type="KEGG" id="mel:Metbo_0461"/>
    <dbReference type="eggNOG" id="arCOG01812">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063609_0_0_2"/>
    <dbReference type="OMA" id="PYVLRHM"/>
    <dbReference type="OrthoDB" id="61255at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
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        <location>
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            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="9">
        <location>
            <begin position="202"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="371" mass="41297" checksum="A9A08987820AD3A3" modified="2011-05-03" version="1">MVFEGLKHFFNRLGGITVDSTKKVGEGVSVPVEKLNGIGNRAGKVRSGIKRSSSKAKESSKGFSFTSKTQSTVKRRSPRVIEKMRMDKDEIEIFKDLIDKKYDRGDTTPKPEDAQKEAYKKASLEELLKEDEKADLEPKLIMVIGGISFVVVFMLMIVLGFGVLVGAVFGLIILMISIIFVFLPNIKKGSRSNEASRELPFALRQMATELRAGIGMHDSMRSVALSGYGALSEEFARALEEIKYGETTEKALTDMSERVNSEGLKRAIYQITRTLSSGGDLAKTLNVIADDTAYEMRMKLKDYSQKLNSFTMIYMFVAILGPVITMIMLIAASTVMGSVLPPMLLLILYLFLFPMIVAFMAFMIKRLEPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9J6</accession>
    <name>F0T9J6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP_bind_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644095.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0492"/>
    <dbReference type="EnsemblBacteria" id="ADZ08744">
        <property type="protein sequence ID" value="ADZ08744"/>
        <property type="gene ID" value="Metbo_0492"/>
    </dbReference>
    <dbReference type="GeneID" id="10276932"/>
    <dbReference type="KEGG" id="mel:Metbo_0492"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OMA" id="MNLDQKQ"/>
    <dbReference type="OrthoDB" id="57500at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="51"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="311" mass="34913" checksum="213ABEE718A51016" modified="2011-05-03" version="1">MNECDKCGSPEIIIKKKQSGQKLCSKCFIESTKKKVLKDIRRQNLIEKGDKVLLGLSGGKDSVMLLDILSTLKNRGIIDLVAVTIDEGIEGYREEGVELAIKNAEKYGVEHRVVSFKQYFGTTLDKIIQNNSLDVRRNACTYCGVFRRWIFNRVSKEVGATKIATGHNLDDETQSIVMSYLEGNIQNLTRIGPKSQSKSDKFTVKIKPLREIPEKEIGLYVIAKELDVHFAGCPYAGESFRAEIGTILKQLTANHPTIMYSTLRGFDKIKPVLKKEFATESVFEVCEICGEPSASRLCKACSFLKQWEDQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K7</accession>
    <name>F0T9K7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644106.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0503"/>
    <dbReference type="EnsemblBacteria" id="ADZ08755">
        <property type="protein sequence ID" value="ADZ08755"/>
        <property type="gene ID" value="Metbo_0503"/>
    </dbReference>
    <dbReference type="GeneID" id="10276943"/>
    <dbReference type="KEGG" id="mel:Metbo_0503"/>
    <dbReference type="eggNOG" id="arCOG06476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036601_1_0_2"/>
    <dbReference type="OMA" id="PMTVIMP"/>
    <dbReference type="OrthoDB" id="51798at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE" evidence="6">
        <location>
            <begin position="17"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE_2" evidence="7">
        <location>
            <begin position="280"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="417" mass="45119" checksum="699521BD297EA96F" modified="2011-05-03" version="1">MNEKHAGNNISSDGLLDFLTELGKALTASGISVVDITAILERIADAYGEEAEILIFPTMILIKIGEHESAPLNAANQKPGILPLNQVSEIYDLVYKVEKAQISPKEAKKCLRQILAEKHCFGPVGILIGYILFSIGIGMLLQPTPEQLIVSGFLGAIIGILLILSRGRNRFMLILPVIAAFIVSTIFLWSLKAGLIAGSVTMLLPALAYFLPGATLTTGMFELAYGEIISGSSRVIYGTAILLLILFGVLIGIQITGFQEPKFFVTNTANALGWWAPYLGVFMFTIGMYLFMSIKNKDLPWVLLIVYVAFFGQIIGNHLLGSLFGAFLGSLLMAISGTIVERQEHRTPSFISIMPAFWILVPGALSFLSLATLFNQNYLTALNYSVDVAMTIVAISLGLLIGAVATEPIKGKIIKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M4</accession>
    <name>F0T9M4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thymidylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644123.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0520"/>
    <dbReference type="EnsemblBacteria" id="ADZ08772">
        <property type="protein sequence ID" value="ADZ08772"/>
        <property type="gene ID" value="Metbo_0520"/>
    </dbReference>
    <dbReference type="GeneID" id="10276965"/>
    <dbReference type="KEGG" id="mel:Metbo_0520"/>
    <dbReference type="eggNOG" id="arCOG01893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_2_2"/>
    <dbReference type="OMA" id="MRFIVID"/>
    <dbReference type="OrthoDB" id="114844at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="1">
        <location>
            <begin position="6"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="22319" checksum="268B3C1D4EE96FDD" modified="2011-05-03" version="1">MRFIVVDGLDGSGKDTQAKLIADKYLANGESVIVRSHPSEDNPYGIMAKKALLGHGKLDHIMASVYYALDVIRSVRVYKESADNVIMVRYLMGVAYLPMPIASLLYKFFITVLPSSEYMFFLDVEPAVLMERLSSRSEKEMFENPDDLVKVRKKALKLTEGWHIIENGQSLEDSQKKIAEILDDLDKKKSLKENLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M6</accession>
    <name>F0T9M6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644125.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0522"/>
    <dbReference type="EnsemblBacteria" id="ADZ08774">
        <property type="protein sequence ID" value="ADZ08774"/>
        <property type="gene ID" value="Metbo_0522"/>
    </dbReference>
    <dbReference type="GeneID" id="10276967"/>
    <dbReference type="KEGG" id="mel:Metbo_0522"/>
    <dbReference type="HOGENOM" id="CLU_2712849_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="72" mass="8234" checksum="6FC7E4E9A43D7FF6" modified="2011-05-03" version="1">MISENYCGGSLNEVAQCLKADQTMMVKFYPEQALPVDVVTYPVFEKYMDLKDSTVVYVTCSKGYYRIQIMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9P2</accession>
    <name>F0T9P2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645272.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1696"/>
    <dbReference type="EnsemblBacteria" id="ADZ09921">
        <property type="protein sequence ID" value="ADZ09921"/>
        <property type="gene ID" value="Metbo_1696"/>
    </dbReference>
    <dbReference type="GeneID" id="10278153"/>
    <dbReference type="KEGG" id="mel:Metbo_1696"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_1_2"/>
    <dbReference type="OMA" id="FCLDIEK"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="20"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="27196" checksum="F5D2FCC237659CE5" modified="2011-05-03" version="1">MKIDEGIVMLEVAGTVPGMFIYPTVMWDSKNMVLVDVGYGGQLDTLKNAFNKEGVDFNKVNKVIITHQDLDHFGGLGDLVDASNNSIDVMTHEDDKPYIQGEKPLVRLNENFLNMLPEDRRNMVKQMYKNFRTVNVDTTLQDEEELDLVGGITVIHTPGHTPGHICLYHHPSKTLIVGDAMNIIDGTLQGPRKDILMEEDYRVALKSLKKLEKFDAENIITYHGGLYKNTNHQSINSLIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S8</accession>
    <name>F0T9S8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09957">
        <property type="protein sequence ID" value="ADZ09957"/>
        <property type="gene ID" value="Metbo_1733"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1733"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207618_0_0_2"/>
    <dbReference type="OMA" id="ILMAMIC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="235" mass="24735" checksum="8AE38422C6D91F29" modified="2011-05-03" version="1">MVKCNDCGYDNDLNASYCENCGKKINGFSNGNYDVEKTETGFSNLTKVLIAVCIVLIVGVGITAGYLIKNNQQTPAVAVNGSNSNSTGTPVSKADGFPVSEAPNLGYQIMKNNGTISSVTYNGVTLDKNQCLYILAKAVVMLNAGQTGNIPINSYGSAANPAGTVTSATIIKSDYVDIASRTVTWMDNYKNAPNYVGITNPGQPDLSPDTTLNMFAKVLSDYKATGQLPSSVSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T5</accession>
    <name>F0T9T5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospholipase C zinc-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09964">
        <property type="protein sequence ID" value="ADZ09964"/>
        <property type="gene ID" value="Metbo_1741"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1741"/>
    <dbReference type="eggNOG" id="arCOG10205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094458_0_0_2"/>
    <dbReference type="OMA" id="YESQVID"/>
    <dbReference type="OrthoDB" id="103413at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="SSF48537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn_dep_PLPC" evidence="1">
        <location>
            <begin position="66"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="25076" checksum="80C52FBD0A8B5F11" modified="2011-05-03" version="1" precursor="true">MNFNKILNLMVVFMISLLIMVPASDAWSWKTHSQVVDVVYYGLPSDVQRNLNLGIMENASNDPDEIFHDFTYHSYPKSYNKSIYWLNLGKTAYDNGDYANASYDYGVACHYISDTFSAPHGVSGESSSLHSKYEDHAKKLTPVASSVNGDLNSLMENGKIEDSKSWDSWIQTNDDSIIQKNLDNGTSVCLQAVKTSVTGNSSEATNTKSESIFDVILNFFSKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z3</accession>
    <name>F0T9Z3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0565"/>
    <dbReference type="EnsemblBacteria" id="ADZ08816">
        <property type="protein sequence ID" value="ADZ08816"/>
        <property type="gene ID" value="Metbo_0565"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0565"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_3_2"/>
    <dbReference type="OMA" id="GSKTHWQ"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="183"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="450" mass="50433" checksum="54D4E70750EA5323" modified="2011-05-03" version="1">MKVLMINPPYNSSKYKFIGLVAPPLGIAYIAAMLEKHGVTVKILDAPALEMDHEGVSKEIKNYSPDIIAITSVTPTIGSALETAKLSKEVCPNAVTVLGGYHPTFTFPEMLKNDFVDIVVKGEGELTMVDLVDALEKGRDLQEVEGIATRDFVTEPRKIIEDLDSLPFPARHLLPMDEYKILNMKLTTGTIISGRGCPYQCSFCASSAMHGHKLRLRSAKSVVDEMEHLVNEHDIEMVAFMDDTFTISKKRVYEICEAIKERGLKNYWGCTARVDTISEDLLKTMKDAGCITMFLGVESADQQVLNEVNKKTNIAKIKETFELTRKYDMRTIASVVLGMPGDTKSSIRNTINFVKQLEPSYAVFSLATPYPGTDFYIKAASENLIKINDWSKYTLLSPVLETVDCSLEELRKLQKKAFTEFYLRPRYILRQTRMDGPIILKTIASIVRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA52</accession>
    <name>F0TA52_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIB</fullName>
            <shortName evidence="8">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tfb</name>
        <name evidence="12" type="ordered locus">Metbo_1780</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1780"/>
    <dbReference type="EnsemblBacteria" id="ADZ10002">
        <property type="protein sequence ID" value="ADZ10002"/>
        <property type="gene ID" value="Metbo_1780"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1780"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_0_2"/>
    <dbReference type="OMA" id="REVACVI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="PTHR11618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="SSF47954"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="11">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="8">
        <location>
            <begin position="142"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="8">
        <location>
            <begin position="236"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="325" mass="36431" checksum="CC75EFAE94972C96" modified="2011-05-03" version="1">MDNTHDPDVSGKEQMKQEVSEIEKKETRCPECGSEQIINDPERGEVACGACGLVIDDNIMDMGPEWRAFDNEQRDKRTRVGAPMTYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVREAASVVYRNAVENKLIRGRSIEGVVAASLYAACRKCNVPRTLDEIAEVSRVGKKEVGRTYRFLTRELNIKLPPTSPVDYIPRFASELNLSGEVQSKAIEIINNAMAKGLTSGKGPTGVAAAALYIASVLLGERKTQRDVAEVAGVTEVTIRNRYKELSEEIEMGLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA53</accession>
    <name>F0TA53_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10003">
        <property type="protein sequence ID" value="ADZ10003"/>
        <property type="gene ID" value="Metbo_1781"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1781"/>
    <dbReference type="eggNOG" id="arCOG10949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_220552_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="34" mass="3820" checksum="BDD78EB49DECB599" modified="2011-05-03" version="1">MAKISGTNKRTRPKKGYKKAGSKRGRAVKNPFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA63</accession>
    <name>F0TA63_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645364.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1791"/>
    <dbReference type="EnsemblBacteria" id="ADZ10013">
        <property type="protein sequence ID" value="ADZ10013"/>
        <property type="gene ID" value="Metbo_1791"/>
    </dbReference>
    <dbReference type="GeneID" id="10278248"/>
    <dbReference type="KEGG" id="mel:Metbo_1791"/>
    <dbReference type="eggNOG" id="arCOG03124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_661591_0_0_2"/>
    <dbReference type="OMA" id="FSNGQQS"/>
    <dbReference type="OrthoDB" id="144288at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="40910" checksum="CF7EC5A682CA3D9D" modified="2011-05-03" version="1" precursor="true">MKGIQIFKILGVLITLLVCLSGSYAANPNEVNTADNVNLQQADQTTTDLGYMADDSGADDSGVDDSGADDSGVDDSGADDSGADDSGVDDSGADDSGADDSGADDSGVDDSGADDSGVDDSGADDSGVDDSGVDDSGADDSGVDDSGVDDSGADDSGVDDSGADDSGADDSGADDSGVDDSGADDSGVDDSGVDDSGVDDSGVDDSGADDSGVDDSGADDSGVDDSYTIDPVYEPLSDVYRTLTLEKFVADDLTTDDSSADDSGSDDTNPTDIYYTLATSEFTDGNAPLAAGGEETDDSGADDSDSGSGEVDDNGYDETGIDDGSSDDNGSDDNGIDDGSFDDNGSDEYTITAEQTGVAGNGGNGGKGGNGGNLSANDTLNSTNSIPMQHTGLPILPALLGVLSLVSGFAINKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA89</accession>
    <name>F0TA89_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogenase</fullName>
            <ecNumber evidence="2">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nitrogenase component I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1817</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645390.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1817"/>
    <dbReference type="EnsemblBacteria" id="ADZ10039">
        <property type="protein sequence ID" value="ADZ10039"/>
        <property type="gene ID" value="Metbo_1817"/>
    </dbReference>
    <dbReference type="GeneID" id="10278274"/>
    <dbReference type="KEGG" id="mel:Metbo_1817"/>
    <dbReference type="eggNOG" id="arCOG03983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148675_0_0_2"/>
    <dbReference type="OMA" id="CYWVDAV"/>
    <dbReference type="OrthoDB" id="95761at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014278">
        <property type="entry name" value="Nase_Fe-Fe_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004349">
        <property type="entry name" value="V/Nase_d_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03139">
        <property type="entry name" value="AnfG_VnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02929">
        <property type="entry name" value="anfG_nitrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="14268" checksum="F736F37A2AFD07C7" modified="2011-05-03" version="1">MDEIQQEKVEKLVNYIMKWSLWQFNSREWDREKQNEEILKKTMQLLCEEPVENETPEDRYYWSEAMILSSDFKTNFEWISQMNKTEIKKLMEDLKNRIDYLTITGSLNAELKVERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI6</accession>
    <name>F0TAI6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645409.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10058">
        <property type="protein sequence ID" value="ADZ10058"/>
        <property type="gene ID" value="Metbo_1836"/>
    </dbReference>
    <dbReference type="GeneID" id="10278293"/>
    <dbReference type="KEGG" id="mel:Metbo_1836"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978644_0_0_2"/>
    <dbReference type="OrthoDB" id="115137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="269" mass="29858" checksum="C38BEC1A36B083E1" modified="2011-05-03" version="1">MILLSNINHSDAYQLNSSTPQVNSVNPGNNSIIPKSQAIKLTFSKSIKLNKNSITLKNMDGKLISTNNKVSGKSLILTPVNQLKPGKYYLALGKGAVTDSYKNGNSNYKSCFTISPISLAQMKDGKSRVERFYAVNHRLPNYVSFGSKKIMINDFEKLLTTQNLKLNKTSSVKTYSITRQVGCIAYNISLSNKVVSSTSKCSCGACGDYVYHTSTYKNYCPNCGRYETLVWNPKGVYEGEWTCSYCDCDYCSACGKEKVHNHPKHLIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK9</accession>
    <name>F0TAK9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1860"/>
    <dbReference type="EnsemblBacteria" id="ADZ10081">
        <property type="protein sequence ID" value="ADZ10081"/>
        <property type="gene ID" value="Metbo_1860"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1860"/>
    <dbReference type="eggNOG" id="arCOG04425">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_3_2_2"/>
    <dbReference type="OMA" id="DNAKERC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="1">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="165" mass="19002" checksum="24A7C84AB931C090" modified="2011-05-03" version="1">MVKKRVLFMCIHNAARSQMAEGLFRHFYGDGFDVYSAGSEPQGVDPLAIKCMDEIGIDISNHRSKSLKEFEGQNFDYVITVCGNSYNACPFFVGGIKYFKQAFEDPSSFEGNEKEKLELFNKVRDELSFWLNDLYYSYLLPDDERVVEEDKDVCCGSIDREFSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL6</accession>
    <name>F0TAL6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1867</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1867"/>
    <dbReference type="EnsemblBacteria" id="ADZ10088">
        <property type="protein sequence ID" value="ADZ10088"/>
        <property type="gene ID" value="Metbo_1867"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1867"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_7_2"/>
    <dbReference type="OMA" id="PHWVWVL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="481" mass="50969" checksum="4B906D551A3F9615" modified="2011-05-03" version="1">MKKIFQKKCINEALDCAKNGDTLKRSIGPLNLTIMGVGGIIGAGIFIVTGVASASSGPALIVSFIIAAIACTFTALCYAEFASMIPIAGSVYTYTYITMGEIWAWIMGWILILQYLIASAAVAIGWSSYVVALVGSFGITLPAIISGPMGVNGSLINLPAVLIILALTAVLVRGAEESAKVNAIIVFVKVAVILLFITLGIYFINPANYHPFTPFGITGIFEGAAMVFFAYLGFDAIASAAEETKNPGRTIPIGIIGSLIISSILYVAVAAVMTGMVPYNLLNTASPVSFALQYVGVQWAFTVITIGAIAGLTTVVLVNMFSKARIIFAMSRDGLLPGVFSNVKDGCQAPVASILLVGAMATIIAAFFSLNSIFELVNVGALTAFMVLALAVIILRIKEPELERSFKCPLVPVIPVMSIIFSLVLISQLKIHILQIFAIWLAVGIIVYFVYGKYRGLFTKPVCGVDDEAEGFEPVDVPTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN9</accession>
    <name>F0TAN9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin B12-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645462.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1891"/>
    <dbReference type="EnsemblBacteria" id="ADZ10111">
        <property type="protein sequence ID" value="ADZ10111"/>
        <property type="gene ID" value="Metbo_1891"/>
    </dbReference>
    <dbReference type="GeneID" id="10278349"/>
    <dbReference type="KEGG" id="mel:Metbo_1891"/>
    <dbReference type="eggNOG" id="arCOG03402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064060_0_0_2"/>
    <dbReference type="OMA" id="YYLGANV"/>
    <dbReference type="OrthoDB" id="52414at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038719">
        <property type="entry name" value="Phycobilisome_asu/bsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="216"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="346" mass="39199" checksum="5614D7B9B80F2158" modified="2011-05-03" version="1">MLKDANELAIEIVDKQFETKNKFYKFYGESEFNKYIEDTEYSLSYLFEAIEASSPELFEDYISWERIFLVNLGMPEELLKERLEVMEDVIIRNLSPERSSLAVKYIEDSIDHLTEPSKYVSFVNEDQPLVKLLEQYIDLLLKGDRHSAGKMVIEAVDNGISVKDIYLNVFQSSLYEIGRLWQENKITVAQEHYFTAATQLIMSQLYPYISRTKKTGKVLVATSVSKELHEIGVRMVADFFEMDGWKTIYLGANTPGDGVIESITTNHADLLLISATISSNIGEVVRLIRNVRSCSQCGEPKIIVGGYPFGVDKELWKKVGADGQAENAENAVLLANSIVSEGYDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP5</accession>
    <name>F0TAP5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GPR1/FUN34/yaaH family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1897</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ10117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1897"/>
    <dbReference type="EnsemblBacteria" id="ADZ10117">
        <property type="protein sequence ID" value="ADZ10117"/>
        <property type="gene ID" value="Metbo_1897"/>
    </dbReference>
    <dbReference type="GeneID" id="10278355"/>
    <dbReference type="KEGG" id="mel:Metbo_1897"/>
    <dbReference type="eggNOG" id="arCOG03176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_3_0_2"/>
    <dbReference type="OMA" id="WKKGNTF"/>
    <dbReference type="OrthoDB" id="110547at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP"/>
    </dbReference>
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        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="20"/>
            <end position="39"/>
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    </feature>
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            <end position="93"/>
        </location>
    </feature>
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        <location>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="192" mass="20729" checksum="737109FBCD0F7115" modified="2011-05-03" version="1">MVEEVKKIVLEDLTANPAPLGLLGFGLTTVLLNIHNAGFYPMNSMILAMGVAYGGIAQIMACWMEYKKGNTFGMVAFGSYGLFWWSFVLLLILPKLGLAAAPDPAALASYLFMWGLFTLVMFIGTLKHSRGLQVVFISLAGLFFLLTAAELTGNPTLTLVAGYEGIFTGLSAVYVGLATVINETYKRDVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX5</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644352.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0750"/>
    <dbReference type="EnsemblBacteria" id="ADZ09001">
        <property type="protein sequence ID" value="ADZ09001"/>
        <property type="gene ID" value="Metbo_0750"/>
    </dbReference>
    <dbReference type="GeneID" id="10277198"/>
    <dbReference type="KEGG" id="mel:Metbo_0750"/>
    <dbReference type="eggNOG" id="arCOG10204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025380_1_1_2"/>
    <dbReference type="OMA" id="GVQGRYY"/>
    <dbReference type="OrthoDB" id="93517at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018674">
        <property type="entry name" value="DUF2142_membrane"/>
    </dbReference>
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        <property type="entry name" value="DUF2142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="32"/>
        </location>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="404" mass="46598" checksum="140FEBCA38936FFB" modified="2011-05-03" version="1">MNIKYPALKPEILFLIIGLIFGVTIALINPMFQVPDEPTHYLKVINLAQGHIFVEKSKVFVDMYSPIPYIVPTIAILLGKLSGLSPAIIFYLGRLANLFLYVSIIYFAIKYTPILKWVIILLALMPMALYEAGSFSSDGFNIAISLLLISFILKLAFDDKIYKVNKNQLLFIFFLGALLALSKEIYIFVLLLFLIIPKHKFVSKKYKYTWFSIIFLFTILIALLWTLIVKGIYVPISPQVYPQKQFIFILTHFITFTSILINTIINNFVYYLTTFVGTFGWKDTGLDTPLSPILVYIYILFLFYIALTDKSEVNMNLKQRICSIMVFLISFVSIFVLEYLTWNNVGNNLIDGVYGRYFIPIAPLLFLGVYSNRIPNFKVNKILVPCFSIIILIISVFMIYHRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB02</accession>
    <name>F0TB02_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclophil_like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="Metbo_1928"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Cyclophilin_TM1367"/>
    </dbReference>
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        <property type="entry name" value="DUF369"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006456">
        <property type="entry name" value="UCP006456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclophil_like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="118" mass="13296" checksum="0C0F63457ED5AEB8" modified="2011-05-03" version="1">MKIEIEVIGKGKAEGVLDDRNPITAKQIFNNLPFEGEANIWLEEVYFDAPIKEEYENPSTSTSKGDISYWPPGPAFCIFYGQSQPASAVNNIGEILSNLELFKEVENDDKIIIRKLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB20</accession>
    <name>F0TB20_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1946</name>
    </gene>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1946"/>
    <dbReference type="EnsemblBacteria" id="ADZ10166">
        <property type="protein sequence ID" value="ADZ10166"/>
        <property type="gene ID" value="Metbo_1946"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1946"/>
    <dbReference type="eggNOG" id="arCOG00933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023791_0_0_2"/>
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    <dbReference type="OrthoDB" id="13164at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="PTHR43306"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="88"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="500" mass="56235" checksum="8975889AC2E8F425" modified="2011-05-03" version="1">MGGMIIIKNTKSLCPICLKVVDAEVYEDNGKILIKKTCSEHGEFINTYWSNSEIYNKAENYDKVGNGIENPQTIADTDCPNNCGICDEHESHTVLGLIDVTNRCNLKCPVCFANAAVSKKLYEPSYEEIRTMLRNLRNNRPVPTPAIQYAGGEPTVRKDLVDLIKLAKEEGFSHTQIATNGVRLARLPSLAQELKDAGLNTVYLQFDGVTEEPYLEIRQKDLLATKIKAIENCRKANLGIVLVPTLLKGINDDQIGDILKFATDNIDIIRGVNFQPVSFAGRTPADEVESQRVTVPDFAEMVQEQTDSQITVEDFYPASSVTPISEFIQAMDGKDAQVTFTCHPHCGTATYVFLDDDKMIPITRFIDVDRFFDLLVNSTEKLEDGGLVAKAKIVARATMELPKTVDMSKSPESVDLRSIITAVFKERSYKALGEFHKRTLLVSCMHFMDPFNFDQDRVRRCVIHYAVPDGRIIPFCSMNTLYRQEIENKFSKPLKEMPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB30</accession>
    <name>F0TB30_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645527.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1956"/>
    <dbReference type="EnsemblBacteria" id="ADZ10176">
        <property type="protein sequence ID" value="ADZ10176"/>
        <property type="gene ID" value="Metbo_1956"/>
    </dbReference>
    <dbReference type="GeneID" id="10278416"/>
    <dbReference type="KEGG" id="mel:Metbo_1956"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_5_0_2"/>
    <dbReference type="OMA" id="FIIIDSG"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="351"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="520" mass="55956" checksum="ADA30F9F10280E42" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBA8</accession>
    <name>F0TBA8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo3</name>
        <name evidence="4" type="synonym">rpoD</name>
        <name evidence="6" type="ordered locus">Metbo_0808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="4">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644410.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0808"/>
    <dbReference type="EnsemblBacteria" id="ADZ09059">
        <property type="protein sequence ID" value="ADZ09059"/>
        <property type="gene ID" value="Metbo_0808"/>
    </dbReference>
    <dbReference type="GeneID" id="10277257"/>
    <dbReference type="KEGG" id="mel:Metbo_0808"/>
    <dbReference type="eggNOG" id="arCOG04241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="OMA" id="ECLRHPE"/>
    <dbReference type="OrthoDB" id="72726at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="262" mass="28898" checksum="C36FDDE093C25A20" modified="2011-05-03" version="1">MEIDIKQKDETHLLFAIEGVDDAFINAIRRISTVEVPTMAIETVEILKNDAKIFDEALAHRLGLVPLKTDLESMVLASDCDCEDYCPTCSVTLLLKEKGPKTVYSGDLQSQDPDVVPVHDTIPLLKLKEGQEVELIAVAKLGIGLEHSKWQPTTTCAYKYYPKITIDSDKCESCGLCASECPRAVYEFDEKANTLEIVDIENCSMCKTCVRGCEAGAITVEGEKGKYLFTIETDGSLSPEKVLTTACEILSSKSDKIIEFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC9</accession>
    <name>F0TBC9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644431.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0829"/>
    <dbReference type="EnsemblBacteria" id="ADZ09080">
        <property type="protein sequence ID" value="ADZ09080"/>
        <property type="gene ID" value="Metbo_0829"/>
    </dbReference>
    <dbReference type="GeneID" id="10277278"/>
    <dbReference type="KEGG" id="mel:Metbo_0829"/>
    <dbReference type="eggNOG" id="arCOG06802">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076546_1_0_2"/>
    <dbReference type="OMA" id="DSYFKAP"/>
    <dbReference type="OrthoDB" id="44384at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="267" mass="29467" checksum="658ED943D834BF0A" modified="2011-05-03" version="1" precursor="true">MDKKIVYINSIAVALLAVAATLSGLFMKNLYKHDTNSIVSQMMGQDIVTLLVAVPLLLISIYLIHGNSMRGRLIWMGTLFYFTYTYASMSFLASYNQLFLVYVAIFSISMYTLLGELVTTKFTGLKSKFNPGKLTKITASFLIFIALMLSLMWLKLIIDSIITGTAPAALEGYTTLVIQALDLGVLVPFAIISAVLLLKNNEWGYILTSIFLIKATLIGTAILSMIVFMVLNGVYVDPGQIIIFTTMTILGILVAFGFYRKINGELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF9</accession>
    <name>F0TBF9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645579.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2009"/>
    <dbReference type="EnsemblBacteria" id="ADZ10228">
        <property type="protein sequence ID" value="ADZ10228"/>
        <property type="gene ID" value="Metbo_2009"/>
    </dbReference>
    <dbReference type="GeneID" id="10278469"/>
    <dbReference type="KEGG" id="mel:Metbo_2009"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_1_2"/>
    <dbReference type="OMA" id="HAMSVTD"/>
    <dbReference type="OrthoDB" id="35407at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="23"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="29130" checksum="D57AE7E3FB3DDDBA" modified="2011-05-03" version="1">MSYINSHYAQIYYNTHGKGDPTIFLHGLSDSSQFFKPLKNNITGIKAIQPDLRGHGESDHDVDISMELLTEDLINLLNELHIKRANILGFSLGSLIAQNFALEFPEHVKSLIICSGYSKCGHELSETFKKLEDLTSQGGVPAFFDEMIKLVYTKDYLLKHREIYGFKDMAVQTNSKAAVLKSLSICRTFDVESRLSEINVPTLIMYGSEDILVPPESSQKMHCKLTHSRILSFPMGHNFFLQENIKKIAVEIQKFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK2</accession>
    <name>F0TBK2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formylmethanofuran dehydrogenase subunit E region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_2052</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645622.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2052"/>
    <dbReference type="EnsemblBacteria" id="ADZ10271">
        <property type="protein sequence ID" value="ADZ10271"/>
        <property type="gene ID" value="Metbo_2052"/>
    </dbReference>
    <dbReference type="GeneID" id="10278514"/>
    <dbReference type="KEGG" id="mel:Metbo_2052"/>
    <dbReference type="eggNOG" id="arCOG00762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087508_0_0_2"/>
    <dbReference type="OMA" id="HGHICAG"/>
    <dbReference type="OrthoDB" id="90623at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026328">
        <property type="entry name" value="FmdE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006578">
        <property type="entry name" value="FwdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FmdE" evidence="5">
        <location>
            <begin position="22"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="DksA C4-type" evidence="4">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="22915" checksum="19551CD5E1E8B2A7" modified="2011-05-03" version="1">MKRIENKGDLIPIKAFKEVTEFHGHVCPGSAIGYKAAEAGLNELESSASSDEEIVAIVENDSCAVDAIQVVTGCTFGKGNLIFLDHGKQVYTFYNRQTNDGVRVALKDSFSVDLLAPKLNQLRAKVHAGNATDLEREDLKNMVKDVANEILEIPYKNMFEVEHVEMEAPSKAKLFKSVKCSKCGEMVSENRTEILNDELVCIPCFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL5</accession>
    <name>F0TBL5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644443.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0842"/>
    <dbReference type="EnsemblBacteria" id="ADZ09092">
        <property type="protein sequence ID" value="ADZ09092"/>
        <property type="gene ID" value="Metbo_0842"/>
    </dbReference>
    <dbReference type="GeneID" id="10277291"/>
    <dbReference type="KEGG" id="mel:Metbo_0842"/>
    <dbReference type="eggNOG" id="arCOG10454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324045_0_0_2"/>
    <dbReference type="OMA" id="HATDHQV"/>
    <dbReference type="OrthoDB" id="122137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="22711" checksum="16754206CDC560B5" modified="2011-05-03" version="1" precursor="true">MKNSKNAIILLVILVLIAVAVSGCTSKSNSTVNQSSTKLAFSNNGTTWFHFDAVIENMTLKNGTVQNYYVDGYIKPNGNVTMDLSSLAGYGNEPLPAGTTVRVLAWKGLFNQTLASNSSDMNLVMQGWSKNLQPQNDDLKYNVTVLNLPVNQLPANITDNTIIINNDPATITGNGSSTEPVFEEEIFTVDGNGKVTMVFVTPATLCNILAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN4</accession>
    <name>F0TBN4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Capsular polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644462.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0861"/>
    <dbReference type="EnsemblBacteria" id="ADZ09111">
        <property type="protein sequence ID" value="ADZ09111"/>
        <property type="gene ID" value="Metbo_0861"/>
    </dbReference>
    <dbReference type="GeneID" id="10277310"/>
    <dbReference type="KEGG" id="mel:Metbo_0861"/>
    <dbReference type="eggNOG" id="arCOG02624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_5_2_2"/>
    <dbReference type="OMA" id="TFYYVTK"/>
    <dbReference type="OrthoDB" id="17209at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="440" mass="51636" checksum="530DCBE944864119" modified="2011-05-03" version="1">MWRKQFIASYYSYQYKNIFENLKKYEKTQFWSLKELEKLQWKKLKNLIIYAYEYVPFYRKLYKSENIDPKDIKSPDQLKKLPIITKRDINDNINDMKSILCQDKDFIKNSSGGSTGENLFFYNDKNRLNIREALTIRGNKWAGLNLGVKNVYLWGSQFDISLQDRFLVKMYNKLAGTLFLSSFNLNDDKMGEYAKKIVKHSPQVLVAYPSSLNTFAQYINQNEILNIDINSIITSGETLFDYQRDLIESTFKCNIFNRYGCREFGPIAHECSEHMGMHLNMEHVYIQFLESKNCKYSNLIITDLDNYVMPFINYEIGDNGNIIEENCNCGRKLPLINIEGRIFDVIVGTNGNHIGGTFWTIFFRHINGIKQFQILQKNKNKIIIKIIVNADYKIEYTEKLINKLKSICGIEMKIEIIIVNEIPLPKSGKHRFIISNLKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN8</accession>
    <name>F0TBN8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0865"/>
    <dbReference type="EnsemblBacteria" id="ADZ09115">
        <property type="protein sequence ID" value="ADZ09115"/>
        <property type="gene ID" value="Metbo_0865"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0865"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="EPIKVFN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="321" mass="35454" checksum="7EF983E1B90D5F1B" modified="2011-05-03" version="1">MKGDKKIKNKNIVVTGGLGFIGSHISEELCKKNDVTIVDNESTGNIKNVQDFQDELSIKIGDVNKLDLNDIFEDVDYVLHQAALPSVPRSIKDPISSNETNITGTLKVLNAAHETGVKKVVFASSSSVYGDTPKLPKVETMPINPKSPYAITKATGELYCNIFEEIYGLPTVSLRYFNVFGPRQDPNSQYSAVIPKFITAIKNGVSPMVYGDGTQSRDFTYVKNVVNANIKACESNNTGIFNIACGRQIILNDLIKILNEIMGKNITPLYVDPRPGDINHSLADIKTAKSIGYNPKDDFINELKLTIEFFWNDTNPKNTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ1</accession>
    <name>F0TBQ1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09128">
        <property type="protein sequence ID" value="ADZ09128"/>
        <property type="gene ID" value="Metbo_0879"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0879"/>
    <dbReference type="eggNOG" id="arCOG13999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159090_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="15622" checksum="A277BDD993F50762" modified="2011-05-03" version="1">MGWSFYDIRSTQKKMEKNGDTMCDEKNFGPMDKESRYKMHHYMKHHAMQSRMHGKDGKMIFMKKVMEQLSDADKKKLLQKKMDMKISMEEQKAEIMKQKKKIIATKLDMKASMSEQKLEMLKMIRDMLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ8</accession>
    <name>F0TBQ8_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644486.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0886"/>
    <dbReference type="EnsemblBacteria" id="ADZ09135">
        <property type="protein sequence ID" value="ADZ09135"/>
        <property type="gene ID" value="Metbo_0886"/>
    </dbReference>
    <dbReference type="GeneID" id="10277335"/>
    <dbReference type="KEGG" id="mel:Metbo_0886"/>
    <dbReference type="eggNOG" id="arCOG02835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907971_0_0_2"/>
    <dbReference type="OMA" id="IDMVIDP"/>
    <dbReference type="OrthoDB" id="77334at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007354">
        <property type="entry name" value="CruF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39419">
        <property type="entry name" value="PTHR39419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04240">
        <property type="entry name" value="Caroten_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="75"/>
        </location>
    </feature>
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        <location>
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            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="143"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="310" mass="35303" checksum="E7F99488558335B9" modified="2011-05-03" version="1">MDILLYLNWFIVLVSIILIFLFKPTKLKKTVYPMYFAFFVVTFFCYLFTLMGSKVLIINMLGALWTYTAFGLVLIHSSLSLGNRKTIVFFIIALIIGLVSELLAVKYGYLGRYYYNPELRPFFFGMVPIMSVFSWATIIYLSYTISNLMLKAGIKKPDLKGNKLHWLILIILLSSISGFVATSIDMILDPVVVAGQGWIWIDGGPYYGIPINNFVGWFFVTFIATFLFRLYESVKPKNDGSLTKGSFFTTLSMILLFVMYFFIYAVSAFKMGSPEYILIGVTTMGPLILITLLITVLKFSGKKLEQNNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS5</accession>
    <name>F0TBS5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_0903</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644503.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0903"/>
    <dbReference type="EnsemblBacteria" id="ADZ09152">
        <property type="protein sequence ID" value="ADZ09152"/>
        <property type="gene ID" value="Metbo_0903"/>
    </dbReference>
    <dbReference type="GeneID" id="10277352"/>
    <dbReference type="KEGG" id="mel:Metbo_0903"/>
    <dbReference type="eggNOG" id="arCOG00253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_2"/>
    <dbReference type="OMA" id="CECLNLP"/>
    <dbReference type="OrthoDB" id="47911at2157"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
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        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="320"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
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            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ09152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="441" mass="48869" checksum="F1027D41E54D8A26" modified="2011-05-03" version="1">MKITIVGTGYVGLVTGACFSEMGNTVYCIDIDDGKVKNLTNGIIPIFEPGLEELVSKNVESGNLMFSTELNFGLKNSSICFIAVGTPMDEDGSADMQYVLEAAREIGQLISNDLIVVNKSTVPVGTADQVKSVIENEMKSRKVSYRVDVVSNPEFLKEGAAVSDFMRPDRVVIGSDNDAVIEVMKELYKPFTINHDRFITMDIRSAEMTKYASNAMLATRISFMNEMANICERVGADINFVREGIGSDKRIGYSFLYPGCGYGGSCFPKDVKALIKTAQDHGYDSTILKGVETVNENQKFSIVSKILKRFGDDLSGKSFAVWGLAFKPETDDMREAASVVIINQLIEHGATIRAYDPKAMDAAKEYYFKSNPDISFHSNKYDAVEGVDSIILVTEWKEFRSPDFDKLKNTAGKTIIFDGRNQYNKTMLEKLGFEYYQIGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX7</accession>
    <name>F0TBX7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">50S ribosomal protein L37e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl37e</name>
        <name evidence="12" type="ordered locus">Metbo_2100</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10 11">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645670.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2100"/>
    <dbReference type="EnsemblBacteria" id="ADZ10319">
        <property type="protein sequence ID" value="ADZ10319"/>
        <property type="gene ID" value="Metbo_2100"/>
    </dbReference>
    <dbReference type="GeneID" id="10278563"/>
    <dbReference type="KEGG" id="mel:Metbo_2100"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OMA" id="RIRRYSW"/>
    <dbReference type="OrthoDB" id="124429at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_L37e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 10 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="7031" checksum="7595C77DDC26CF7A" modified="2011-05-03" version="1">MKGTPSFGKRNKKTHIRCRRCGKNSYNARKKYCAACGFGRSAKIRSYNWQNKKLNGYRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ6</accession>
    <name>F0TBZ6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="5">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">srp19</name>
        <name evidence="6" type="ordered locus">Metbo_2119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645689.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2119"/>
    <dbReference type="EnsemblBacteria" id="ADZ10338">
        <property type="protein sequence ID" value="ADZ10338"/>
        <property type="gene ID" value="Metbo_2119"/>
    </dbReference>
    <dbReference type="GeneID" id="10278582"/>
    <dbReference type="KEGG" id="mel:Metbo_2119"/>
    <dbReference type="eggNOG" id="arCOG01217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="OMA" id="VIWPAYL"/>
    <dbReference type="OrthoDB" id="121943at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="PTHR17453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SSF69695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="91" mass="10595" checksum="F61CC4D3A7CB8449" modified="2011-05-03" version="1">MRATIWPVYIDAKKTKHEGRKVSLEHAITAPKLREISRAAKKLGLNPDVEKDKSYSKSWWENSGRVTVDKTMPKREILMKVSNIIRGYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ7</accession>
    <name>F0TBZ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase DHHA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645690.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2120"/>
    <dbReference type="EnsemblBacteria" id="ADZ10339">
        <property type="protein sequence ID" value="ADZ10339"/>
        <property type="gene ID" value="Metbo_2120"/>
    </dbReference>
    <dbReference type="GeneID" id="10278583"/>
    <dbReference type="KEGG" id="mel:Metbo_2120"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OMA" id="ACGAYIP"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA1" evidence="1">
        <location>
            <begin position="359"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="465" mass="51945" checksum="30A0BCA2052D75CD" modified="2011-05-03" version="1">MLKSNPMNLSFEKAHDLIKKSEDIKIYTHIDCDGVTAGAVLSSMLDKLGKDHEVEFISLDQMDDLVTENELTIFSDLGSGQEMDKLCTSNSKTLILDHHPPIRKLDNYLNGQLIELNPHHHNIDGSYEVSGGGMTYLLARTFGYTDLSWMGVLSAVGDMQNSFSGKLIGLNSSILQESIQQNLISSQNDLAIYGRQTRPLYIALSYFGDVNLPITNNKTECQMLLNKLDIPMKNGRKYRSLCDLSVEEKGRLFSELVRMLSLEVPSRYVKYVPKLISGDSYEFLMEEKYSPLRDASEFSTAVNACGRHKHHKVAMNVLKGDRGASLDEMEHLALEHRRYLAQKMEWIRGDERITPMNNLQYFEGSEIKSEVIGTIAGMILSYGDWKKPIVGFTPIGEEKEGIKVSLRCSRLLAYDGIHFGNLIRKVAKKVGGNGGGHSVACGAYIPEGSTAKFLKIFDESLKGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC03</accession>
    <name>F0TC03_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2126"/>
    <dbReference type="EnsemblBacteria" id="ADZ10345">
        <property type="protein sequence ID" value="ADZ10345"/>
        <property type="gene ID" value="Metbo_2126"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2126"/>
    <dbReference type="eggNOG" id="arCOG02149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070025_2_1_2"/>
    <dbReference type="OMA" id="IPMHIRA"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13866" checksum="5C41D8011CE54D96" modified="2011-05-03" version="1">MSDRYEKGLKNIEKIHPEASKALMKNLEDIAPDLGKFVIEFPYGEVYDRPGLDLKSREIATVAALTALGDTKPELKDHIKGSLNVGCSRQEIIEVIMQMAVYAGFPRAINGISAAKEVFEELDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC08</accession>
    <name>F0TC08_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="7">GSA</shortName>
            <ecNumber evidence="7">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="7">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hemL</name>
        <name evidence="9" type="ordered locus">Metbo_2131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645701.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2131"/>
    <dbReference type="EnsemblBacteria" id="ADZ10350">
        <property type="protein sequence ID" value="ADZ10350"/>
        <property type="gene ID" value="Metbo_2131"/>
    </dbReference>
    <dbReference type="GeneID" id="10278594"/>
    <dbReference type="KEGG" id="mel:Metbo_2131"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="OrthoDB" id="10264at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="420" mass="46192" checksum="5892A335B85EA5D3" modified="2011-05-03" version="1">MNSKQLFEESKKFLPGGVDSPVRAIKPYPFFAEKAEGSKLTDVDGNSYIDYCLAYGPMVLGHSNPEVVSEVEKQLVKGSAYGVPTEKEIELAKMVVNRVPCADMVRFVNSGTEATMSAIRLARAAKSKNKIIKFEGAYHGAHDNVLVKSGSGAAGLPDSPGVPEETTRNTLLIPFNNEESVIEIINKNKDSIAAIIIEPIMGNVGLIPPKNGFLEFLRKITLENDITLIFDEVITGFRIAQGGAQEYFKVTPDLVTFGKILGGGFPMGAITGKKEYMEMIAPSGSVYQAGTFNGNPISITAGLATLKQLDQQFYSDMNSKGKNFREGIRNILEDKNLNYQVAGVSSMFQIYLTDKTVWNYDDAKTADTDKFETYFKTLLKEGVFVPPSQFECCFLSKMHDAEDIDKTLNIIDTAMKQIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC18</accession>
    <name>F0TC18_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644520.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0920"/>
    <dbReference type="EnsemblBacteria" id="ADZ09169">
        <property type="protein sequence ID" value="ADZ09169"/>
        <property type="gene ID" value="Metbo_0920"/>
    </dbReference>
    <dbReference type="GeneID" id="10277369"/>
    <dbReference type="KEGG" id="mel:Metbo_0920"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_1_2_2"/>
    <dbReference type="OMA" id="AGETTWH"/>
    <dbReference type="OrthoDB" id="43377at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="1">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="31297" checksum="A96F80B4D5E32349" modified="2011-05-03" version="1">MKVMIIGSEGMLGHDLVAVLSPLHEVVTTTIDTLDITDIDKTIKTVKKNNPDVLIHAAAITDVDGSESNPDLAYKVNAIGTRNVAVACKEVGASMVYISTDYVFDGTNTTPYNEYDQTKPLGVYGKTKHTGETYVRDTLSKFYIVRTAWLYGYHGPNFVTTMLNLAKNTKDISVVDDQVGSPTYTVDLANAISTIINRSSYGIYHFTNTDHCSWFDFATEIFKQAGVEVNLKPVTTEEFPRPAPRPKYSVLNHYSWKMEGYPPIRNYKEALKDYMKLLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC33</accession>
    <name>F0TC33_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0937</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644535.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0937"/>
    <dbReference type="EnsemblBacteria" id="ADZ09184">
        <property type="protein sequence ID" value="ADZ09184"/>
        <property type="gene ID" value="Metbo_0937"/>
    </dbReference>
    <dbReference type="GeneID" id="25394721"/>
    <dbReference type="KEGG" id="mel:Metbo_0937"/>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OMA" id="ELCMVEE"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="205"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="303"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="513" mass="60016" checksum="B3B51DE2A691805C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC65</accession>
    <name>F0TC65_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644567.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0969"/>
    <dbReference type="EnsemblBacteria" id="ADZ09216">
        <property type="protein sequence ID" value="ADZ09216"/>
        <property type="gene ID" value="Metbo_0969"/>
    </dbReference>
    <dbReference type="GeneID" id="10277418"/>
    <dbReference type="KEGG" id="mel:Metbo_0969"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="OMA" id="IYTEYSM"/>
    <dbReference type="OrthoDB" id="17570at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="224" mass="25204" checksum="F6E0C328F0CA443C" modified="2011-05-03" version="1">MRTFVVVPAYNEEKTIEKVLHDLSIMGFEFVVVDDGSMDGTNNIVKKFIEENNHGFICRHLLNRGLGAAIKTGIEASLEENADVIVTFDADGQHDPNDIYTVINPIIHQEADVCIGTRDFDDMPRSKKYGNSVMNLITMIFYGIKVGDSQSGLRAFNKKAAKAIIINSRDYGVSSEIIGEIKRQNLELKEVPMKTIYTDYTMKKGTNLFEGLKILSKLIFKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB6</accession>
    <name>F0TCB6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation protein HypE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645734.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2168"/>
    <dbReference type="EnsemblBacteria" id="ADZ10383">
        <property type="protein sequence ID" value="ADZ10383"/>
        <property type="gene ID" value="Metbo_2168"/>
    </dbReference>
    <dbReference type="GeneID" id="10278631"/>
    <dbReference type="KEGG" id="mel:Metbo_2168"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049733_0_0_2"/>
    <dbReference type="OMA" id="CGNGGKE"/>
    <dbReference type="OrthoDB" id="34102at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="42"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="166"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="336" mass="35193" checksum="23F0BD6E97B74A17" modified="2011-05-03" version="1">MKIGMSHGAGGGIMQGLISEIILENLKNKSVNGGVGLDDLDDGASIPMDDYEIVVSTDSHTVDPIFFPGGDIGTISIAGTVNDVSVMGAKPLAITNAMVISEGFSGEDFEKIIKSMDAVCREAGVSIITGDTKVMEHDKLDKIIIATTGIGIVKKGEIKRDSGLEIGNKIILTGSVGDHGISLMSYREGFGFETDLQSDVAPVWGMVEAALKVGGVTAMKDPTRGGIANALNELASKSGVGMLIDEEKIPLKAEVVAASEMLGLDPYEVANEGKVVMGVEADKADETLEAIRKTKYGKDAQIIGEVTDNVHVVIETLLGGKRILEAPIADPVPRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF8</accession>
    <name>F0TCF8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645776.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2211"/>
    <dbReference type="EnsemblBacteria" id="ADZ10425">
        <property type="protein sequence ID" value="ADZ10425"/>
        <property type="gene ID" value="Metbo_2211"/>
    </dbReference>
    <dbReference type="GeneID" id="10278677"/>
    <dbReference type="KEGG" id="mel:Metbo_2211"/>
    <dbReference type="eggNOG" id="arCOG04843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067987_0_0_2"/>
    <dbReference type="OMA" id="VPQWHYS"/>
    <dbReference type="OrthoDB" id="77659at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018216">
        <property type="term" value="P:peptidyl-arginine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021172">
        <property type="entry name" value="UCP006607_RNA_methylase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PTHR11006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006607">
        <property type="entry name" value="RNAmts_UCP006607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="29076" checksum="39B27B008686241C" modified="2011-05-03" version="1">MTLVNTPYHHNLRSDYERLAGFFEAIKEKSKGTVFDLGTGSGVLSSWAAQYADKVVAVEINRTTSKIAQRNLEAFNNVEVVNADASKYQFSTKADVIICEMLDTALIDEEQVPVLNSALKYLKEDGVIIPCGIINCIEPVESSCEHISYEEDGFPKNSVLGDPIFYSKLMFSKPVEPKFKQMFMIKINRKGIVNGLKLTTFTLITEKIICGPTPMLNPPLIIPLKKINVVENDMLEVNLSYEMGGGLESVRARAEKIHPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG2</accession>
    <name>F0TCG2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0992</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + beta-nicotinamide D-ribonucleotide + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644590.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0992"/>
    <dbReference type="EnsemblBacteria" id="ADZ09239">
        <property type="protein sequence ID" value="ADZ09239"/>
        <property type="gene ID" value="Metbo_0992"/>
    </dbReference>
    <dbReference type="GeneID" id="10277441"/>
    <dbReference type="KEGG" id="mel:Metbo_0992"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OMA" id="NSVWVSH"/>
    <dbReference type="OrthoDB" id="90722at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19907" checksum="A6C02D75D0E9F20A" modified="2011-05-03" version="1">MRGLLVGRMQPVHEGHLEVIKRILDEVDELIIGIGSAQLSHTVKDPFTAGERVMMLTKALTENDIPASKYYIIPIQDVACNSIWVAHVKMLTPPFQHVYSGNFLVQQLFREGGFEVTVPPLYNRKTYSGTEVRKRMLNDEDWESLIPSSVKDVIYEIDGINRLKQLSRRELNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U5</accession>
    <name>F0T5U5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1093</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644689.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1093"/>
    <dbReference type="EnsemblBacteria" id="ADZ09338">
        <property type="protein sequence ID" value="ADZ09338"/>
        <property type="gene ID" value="Metbo_1093"/>
    </dbReference>
    <dbReference type="GeneID" id="10277542"/>
    <dbReference type="KEGG" id="mel:Metbo_1093"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="OMA" id="NEYNFVA"/>
    <dbReference type="OrthoDB" id="97465at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="51"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="28558" checksum="5B000F5A5421092A" modified="2011-05-03" version="1">MKKYDYTTIFFAIMGSFLVLFILIPILNLMVSSNLGAISANLHDPQVISSLFVSVYSALIATIIALIFGVPLAYILARHDFFGKGLVESIIDIPIVIPHTVTGIALLLIFTSTGIIGAPLSKLGIIFTNSISGIILAMLFVSASFVVNSAREGFESVDPRMEKVARTLGSGTLRTFFVISFPLALRSIVVGAILCWARAISEFGAIIILAFYPLAASTLIYERFVQFGLSDSTPVAIILISICLALFIVVRLLIKGWRSYDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V5</accession>
    <name>F0T5V5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UvrABC system protein A</fullName>
            <shortName evidence="13">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrA</name>
        <name evidence="16" type="ordered locus">Metbo_1103</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644699.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1103"/>
    <dbReference type="EnsemblBacteria" id="ADZ09348">
        <property type="protein sequence ID" value="ADZ09348"/>
        <property type="gene ID" value="Metbo_1103"/>
    </dbReference>
    <dbReference type="GeneID" id="10277553"/>
    <dbReference type="KEGG" id="mel:Metbo_1103"/>
    <dbReference type="eggNOG" id="arCOG04694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_2"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="721at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0742">SOS response</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="371"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="612"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="744"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="645"/>
            <end position="652"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ09348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="968" mass="108044" checksum="CD14E4772DA96AD1" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z9</accession>
    <name>F0T5Z9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable phosphoglucosamine mutase</fullName>
            <ecNumber evidence="5">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glmM</name>
        <name evidence="11" type="ordered locus">Metbo_2292</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645857.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2292"/>
    <dbReference type="EnsemblBacteria" id="ADZ10506">
        <property type="protein sequence ID" value="ADZ10506"/>
        <property type="gene ID" value="Metbo_2292"/>
    </dbReference>
    <dbReference type="GeneID" id="10278758"/>
    <dbReference type="KEGG" id="mel:Metbo_2292"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OMA" id="KCSQVMY"/>
    <dbReference type="OrthoDB" id="58941at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="158"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="260"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="391"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="5">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="5">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="451" mass="49018" checksum="DE0CF137A0E909DD" modified="2011-05-03" version="1">MSLEIPKLFGTSGIRGKVGEDISLELALDVGRAIASYIGGEGGKIVLGYDSRTSNIMMENAITAGILECGCNVVKLGMAPTPLVGYATIKLNADAGVMITASHNPPEYNGIKLWNPDGMAYRQEQERTIEEIVHKKIFKTVSWQDIGLIEENEDVVNDYIDDILKQITIKPGTKVVVDCANGAASYLSPLILRKAGCDVVSINSHPDGFFPGRMPEPSQKNLQELMKVVKATGAEIGIAHDGDADRMIAIDEKGNMADFDKLLAIVSREIGGVVVTTVDASLCTDKCMEEVNGTVIRTKVGDVHVAESIVDASASFGGEPSGTWIHPNFCMCPDGILSALRVIELVQHNGSLSKQLSSVPSFPTIRDKIICEDYQKEIIMLKVQDELHNLFDDVVDVNRIDGVRISMVDGSWVLIRPSGTESYVRITLEATSDEKACMIRDQCAEFIEDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T642</accession>
    <name>F0T642_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIB</fullName>
            <shortName evidence="8">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tfb</name>
        <name evidence="12" type="ordered locus">Metbo_2336</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2336"/>
    <dbReference type="EnsemblBacteria" id="ADZ10549">
        <property type="protein sequence ID" value="ADZ10549"/>
        <property type="gene ID" value="Metbo_2336"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2336"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OMA" id="DHDQRMK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
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        <location>
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        <location>
            <begin position="1"/>
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        <location>
            <position position="35"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
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        <ligand>
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            <position position="57"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010857"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51134"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ10549.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="37301" checksum="C9D7B4EA0B1E520E" modified="2011-05-03" version="1">MKHEVVNEGISEPEVSEKEQMKQDVSEIEKKETKCPECGSEKLINDHERGEVVCGACGLVIDDNIVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVREAASVVYRNAVENKLIRGRSIEGVVAASLYAACRRCNVPRTLDEIAEVSRVSKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELNLSGEVQSKAIEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLDMGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T663</accession>
    <name>F0T663_METLA</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacteriales</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
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    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_2357"/>
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    </dbReference>
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    <dbReference type="OMA" id="FKHREDM"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
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        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="16844" checksum="1A520FB7066AA89D" modified="2011-05-03" version="1">MKITPKNMIQTWLKQSTNKISIQFFRYIFVGGTAFLVDFGSLYILTEYFGIFYLLSAAIAFTLGLIVNYLLSVRWVFNKHTLDNRFHEVGIFTMVGIVGLGLNEVIIWFFTSNMHLFYIYSKIISAVLVLFWNFFARRYTIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T675</accession>
    <name>F0T675_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09390">
        <property type="protein sequence ID" value="ADZ09390"/>
        <property type="gene ID" value="Metbo_1146"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1146"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691558_0_0_2"/>
    <dbReference type="OMA" id="SACDSDY"/>
    <dbReference type="OrthoDB" id="115137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="155" mass="17236" checksum="643B9DC6DEDF8650" modified="2011-05-03" version="1" precursor="true">MMLSAISMVMVPVAFATSVGAKDVVVNNQYVQATAICSCGSYEYDHYITGTFLNYCPQCHSYGTLEFNPKGTPEGEWTCSKCNSDYCAADGKEKIDGSKYCLTQYNVTTTNTHNSTQNNTTQNQTLIQSEAMLKTFHQTILEQLALLNGKAFYEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H0</accession>
    <name>F0T6H0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps7</name>
        <name evidence="9" type="ordered locus">Metbo_2391</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645955.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2391"/>
    <dbReference type="EnsemblBacteria" id="ADZ10604">
        <property type="protein sequence ID" value="ADZ10604"/>
        <property type="gene ID" value="Metbo_2391"/>
    </dbReference>
    <dbReference type="GeneID" id="10278858"/>
    <dbReference type="KEGG" id="mel:Metbo_2391"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="80498at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_S7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_S5/S7_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_S7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="8">
        <location>
            <begin position="45"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="187" mass="21396" checksum="C1B6B7FD84652433" modified="2011-05-03" version="1">MSFKVFDKWELEEVKVEDLGLVNYICLDEILVPHTLGRHVKRQFAKSKVSIVERLMNKIMRTQRNSGKKNKSYNIVKGALDIINQRSKQNPVQVLVKAVENTAPREETTRIKYGGIGYQVAVDIAPQRRVDLAVGFLTRGALQSSFKRKRSVEECLADELLYAAEYDTRSFAIGKKEEKERIARSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H7</accession>
    <name>F0T6H7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Metbo_2398</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645962.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2398"/>
    <dbReference type="EnsemblBacteria" id="ADZ10611">
        <property type="protein sequence ID" value="ADZ10611"/>
        <property type="gene ID" value="Metbo_2398"/>
    </dbReference>
    <dbReference type="GeneID" id="10278865"/>
    <dbReference type="KEGG" id="mel:Metbo_2398"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_2"/>
    <dbReference type="OMA" id="GKICPSE"/>
    <dbReference type="OrthoDB" id="14180at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="12">
        <location>
            <begin position="17"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="11">
        <location>
            <begin position="194"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="13">
        <location>
            <begin position="411"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="500" mass="56857" checksum="7E0C189CF3B9C3A6" modified="2011-05-03" version="1">MVKNAWKLVDAFFDEYNLVDHHIKSYNDFVNHRIQDIIDITEPIVLEQGEYTIKTGNVEIKKPFIKEADGSNSKIFPTEARLRNLTYSAHMYLEMALTKKGDEEQEMENVYIGELPVMLKSNICHLNGLSEEEIDDVGEDPQDPGGYFIVNGSERAIVTMEEIAPNKIILERIGELEDRRARAIVTSIKSGFRARITLEYRKPRKKGVFLRISFPYVPGEIPLVVLLRALGFETDQDLITSVSDENDIQFLLIDDIQTSEIMTQYDAIKYIGNRVAKGMTEEYRIKRAEDVIDKYLLPHIGVDPSQRAEKATYLAEMTEMLLQVIFETREPHDKDHYANKRLRVSGDLMEDLFRVAFTSLTRDMTYQLERSLARGKEPSVKQAVRSDVLTENIKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHLKRVVSPLSRSQPHFEARDLHPTQFGKICPNETPEGPNCGLVKNLAILSKISEGSDPEELEEIIKKMGVIKPVQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J4</accession>
    <name>F0T6J4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nitrogenase protein alpha chain</fullName>
            <ecNumber evidence="8">1.18.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2415</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[7Fe-Mo-9S-C-homocitryl] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30409"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[8Fe-7S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21143"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645979.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="SMR" id="F0T6J4"/>
    <dbReference type="STRING" id="877455.Metbo_2415"/>
    <dbReference type="EnsemblBacteria" id="ADZ10628">
        <property type="protein sequence ID" value="ADZ10628"/>
        <property type="gene ID" value="Metbo_2415"/>
    </dbReference>
    <dbReference type="GeneID" id="10278887"/>
    <dbReference type="KEGG" id="mel:Metbo_2415"/>
    <dbReference type="eggNOG" id="arCOG00598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_1_0_2"/>
    <dbReference type="OMA" id="ILTNRGC"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005974">
        <property type="entry name" value="Nase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010143">
        <property type="entry name" value="Nase_comp1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43457">
        <property type="entry name" value="PTHR43457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01284">
        <property type="entry name" value="alt_nitrog_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01862">
        <property type="entry name" value="N2-ase-Ialpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="7" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="10">
        <location>
            <begin position="48"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="496" mass="55761" checksum="98BD04F0CA87C943" modified="2011-05-03" version="1">MPYKLLEVDKKIPQRKNHVYVKHCSDPDDEIPDCNTKTVPGSMSERGCAFAGVKGVITGAIKDVIHVVHSPIGCTSYGYGTKRYPTTPDMPNGEKFPIENFNLKYILGTDLQESDVVFGGMKKLKKSIIEAHKEFPEATAIYTYSTCTTGLIGDDLDAVAKEASEELGIQVVAFNAPGFCGPSQSKGHHVGNLTLFENLVGTKEPLETTPYDVNLIGEYNIDGDLWIIKEYLEEMGINLLSKFTGDCSHDEICWMHRAKLSLVRCQRSATYIADLIEEKYGVPYIKVDFFSTKYCAENLRTIGKYFGLEEEAEKVIESRMEKVAPEIEFYKSKLQGKRVFIFSGGPKSYHLSKPLEEELGMETVAVASQFEHKDGYEKMKSRVKEGTLIVDDPNSLEFEEIIQVYKPDLILAGVKEKYLAHKLGVPCLLIHSYENGPYMGFEGFVNLAKDMYRNIYSPVWNMLEFEEEPAVGENTEVQEDSSEKQENKPENTGELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K6</accession>
    <name>F0T6K6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CDP-diacylglycerol/serine O-phosphatidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2427</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645991.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2427"/>
    <dbReference type="EnsemblBacteria" id="ADZ10640">
        <property type="protein sequence ID" value="ADZ10640"/>
        <property type="gene ID" value="Metbo_2427"/>
    </dbReference>
    <dbReference type="GeneID" id="10278899"/>
    <dbReference type="KEGG" id="mel:Metbo_2427"/>
    <dbReference type="eggNOG" id="arCOG00671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_3_1_2"/>
    <dbReference type="OMA" id="YSICGML"/>
    <dbReference type="OrthoDB" id="68873at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="26166" checksum="0EF8C1E90318EDC5" modified="2011-05-03" version="1">MNIKNFMSYADLVSLANASFGFLSIVMAFKGYLDLAAQFMLIAVIFDSLDGWVARRTKRVDQYGFGENIDSLSDVISFGVAPGMLLITATSTFGIPYLNIGVALLILLCGVLRLARFNVIVNSGEVKDEKFVGLPIPTTALILGSFYLSGFFQADLAMLIMVVISLMMVSTVEYPKFRSSSVVVIGSLLIFLTLLPQNFSSIIANIPAKLLFIVSLIYLITVPFMGLYSKLRRSGPNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6L8</accession>
    <name>F0T6L8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl coenzyme M reductase system, component A2</fullName>
            <ecNumber evidence="5">3.6.3.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.17" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646003.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2439"/>
    <dbReference type="EnsemblBacteria" id="ADZ10652">
        <property type="protein sequence ID" value="ADZ10652"/>
        <property type="gene ID" value="Metbo_2439"/>
    </dbReference>
    <dbReference type="GeneID" id="10278911"/>
    <dbReference type="KEGG" id="mel:Metbo_2439"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="OrthoDB" id="25719at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="282"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="319"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="533" mass="59477" checksum="849D9296AA1213BD" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N0</accession>
    <name>F0T6N0_METLA</name>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0010"/>
    <dbReference type="EnsemblBacteria" id="ADZ08263">
        <property type="protein sequence ID" value="ADZ08263"/>
        <property type="gene ID" value="Metbo_0010"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0010"/>
    <dbReference type="eggNOG" id="arCOG04507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185152_1_1_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
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    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
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    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="48" mass="5629" checksum="60CADAE94FA98EAA" modified="2011-05-03" version="1">MAIDPICKMDVDEKTAKWVSEYNGKKYYFCAPGCKKTFEEDPEKYAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q5</accession>
    <name>F0T6Q5_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Metbo_0035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0035"/>
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        <property type="protein sequence ID" value="ADZ08288"/>
        <property type="gene ID" value="Metbo_0035"/>
    </dbReference>
    <dbReference type="GeneID" id="10276457"/>
    <dbReference type="KEGG" id="mel:Metbo_0035"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046973_1_0_2"/>
    <dbReference type="OMA" id="SVYSFCM"/>
    <dbReference type="OrthoDB" id="10986at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="370"/>
            <end position="457"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="465" mass="50949" checksum="DCCCB55332AC6FF7" modified="2011-05-03" version="1">MDYDVIIIGAGPAGIFAANELADNMKVVVVDKGRGLDHRICRALEDGKCRKCYPCNITGGLGGAGGLSDGKLNLKPDIGGDLEEFVDTDEAWKIVRQIDELFLKHGASKELFAPEADKIAPIMKQAAANGINFIPIVQRHMGSDKTPPIINSIKKELENKGVEFLLDTEVIDIIADGELRGALLKNKDGEFTFKTHYVLAAPGRVGAKWLYSQAQKLEIPVRHNPVDLGVRVEIPQIVMDELTKINWDPKFHIITKTYDDFVRTFCVCNKGFVVEEVYDDFVGVNGHSMINKISENTNFAFLVRVELTEPVENTSEYASSIANLANTLGGGKPLIQRLGDLRKGRRSTWRRLERSNVTPTLKSVTPGDIAMALPHRVVVDIIEGLESLNNVIPGVASDSTLIYAPEIKLYAMRLELDKNLKTRVDRLYAAGDGAGVSRGIVGAAATGIVAARNILENYLKTVKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S0</accession>
    <name>F0T6S0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HGSNAT_cat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643654.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0050"/>
    <dbReference type="EnsemblBacteria" id="ADZ08303">
        <property type="protein sequence ID" value="ADZ08303"/>
        <property type="gene ID" value="Metbo_0050"/>
    </dbReference>
    <dbReference type="GeneID" id="10276473"/>
    <dbReference type="KEGG" id="mel:Metbo_0050"/>
    <dbReference type="eggNOG" id="arCOG04370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067755_0_1_2"/>
    <dbReference type="OMA" id="AMASYHF"/>
    <dbReference type="OrthoDB" id="69375at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012429">
        <property type="entry name" value="HGSNAT_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07786">
        <property type="entry name" value="HGSNAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="HGSNAT_cat" evidence="2">
        <location>
            <begin position="8"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="255" mass="29768" checksum="C635B0C4FEF29F9C" modified="2011-05-03" version="1">MEKNLNVRFWEVDSLRGLAVVCMICYHFLFDLAFFGVISWDVSSGFPWFFARITALTFIFLMGVSLTLSSSRSKLSGRYLHKSLFKKYLQRGCKIFALGLFITAITWIFIPQEFIIFGILHFIGISIILAYPFLKRKYLNLVAGISIILIGIYLQTLSFNFNWLMWLGFVPNSLNTVDYFPIFPWFGIVLLGIFFGGILYKNYKRQFKLPDWSDNYFFKGSAFLGRHSLVIYLIHQPIIIIVLYFLGVVNINYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W2</accession>
    <name>F0T6W2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1240"/>
    <dbReference type="EnsemblBacteria" id="ADZ09482">
        <property type="protein sequence ID" value="ADZ09482"/>
        <property type="gene ID" value="Metbo_1240"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1240"/>
    <dbReference type="HOGENOM" id="CLU_2103516_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="13176" checksum="69166FABBB117AE6" modified="2011-05-03" version="1">MFPIKNSKNAAYKGQLVGNNVQNFHNGNVVRANSAKGTVWDDNNNLHMSMQVLNPVSALNSYKTNGNSAHGKTWYRQFGSYIKLYITRVHSPQYEMVTKKTVWYIGTMYNIWNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z8</accession>
    <name>F0T6Z8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH dehydrogenase (Quinone)</fullName>
            <ecNumber evidence="3">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644869.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1276"/>
    <dbReference type="EnsemblBacteria" id="ADZ09518">
        <property type="protein sequence ID" value="ADZ09518"/>
        <property type="gene ID" value="Metbo_1276"/>
    </dbReference>
    <dbReference type="GeneID" id="10277727"/>
    <dbReference type="KEGG" id="mel:Metbo_1276"/>
    <dbReference type="eggNOG" id="arCOG01547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="OMA" id="TFAECIE"/>
    <dbReference type="OrthoDB" id="4328at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="117"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="279"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="43169" checksum="BFB735C4597597D1" modified="2011-05-03" version="1">MILPIGPVHPGLKEPLRLKLKTRGEKVLSAEIDYGYIHRGIERIMQGKTWQKGIFLSERVCGICSYVHTQTFAETFEKIAGEYAPLRAQYLRVLTNELDRIQSHLLANSTYFKAVEHETLFMYMLALREPVMDAIELLTGNRVNMGWNVVGGVKMDIKQTHMDQIMQIMNKLETDYGRYVQIYEDGPLVGGLRSKDVGVMTREEAIMARAVGPIGRASGLEHDVREQHHTYKDNFDWNVVWRKEGDNFARTMNRFNEVWESISLIKQVINNIPEGDVRKKIDIPAGVAEWRNEAPRGEVTYSIETNGNLIKNISIRTPSIMNIDACAKYMLNDVPTVADAVATYATSDPCIACAERVTILDESGRVLDFHGVHNINSKLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T738</accession>
    <name>F0T738_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein AfpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646023.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2459"/>
    <dbReference type="EnsemblBacteria" id="ADZ10672">
        <property type="protein sequence ID" value="ADZ10672"/>
        <property type="gene ID" value="Metbo_2459"/>
    </dbReference>
    <dbReference type="GeneID" id="10278931"/>
    <dbReference type="KEGG" id="mel:Metbo_2459"/>
    <dbReference type="eggNOG" id="arCOG01705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="OMA" id="IYPPDNK"/>
    <dbReference type="OrthoDB" id="72619at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014072">
        <property type="entry name" value="Archaeoflavo_AfpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02699">
        <property type="entry name" value="archaeo_AfpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="9"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="191" mass="21469" checksum="7DAECF2C9CFD5FD2" modified="2011-05-03" version="1">MIVKGKKHKVAWGITGSGEKILETVEIMEQMKKKYKNTFDIRVFISKAGDQVLKYYNLSNTLETLFDKTWTEINANSPFLAGKIQLGTFDFLLIAPATSNTVAKISLRIADTLLTNAAIMGQKTSTPIYIMPTDFREGETVTKLPNGKDLVLNITRDDAKHVENLSNMPNTTVFEHPSEIPSIFEKHSKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T762</accession>
    <name>F0T762_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646047.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2483"/>
    <dbReference type="EnsemblBacteria" id="ADZ10696">
        <property type="protein sequence ID" value="ADZ10696"/>
        <property type="gene ID" value="Metbo_2483"/>
    </dbReference>
    <dbReference type="GeneID" id="10278957"/>
    <dbReference type="KEGG" id="mel:Metbo_2483"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_4_2"/>
    <dbReference type="OMA" id="EVQIHAF"/>
    <dbReference type="OrthoDB" id="86909at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="PTHR43267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="19"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="27833" checksum="10784D6DDED6E776" modified="2011-05-03" version="1">MSNDYDENIYWEMINRQKGILNKKEQLKLKDSVVTVIGCGGIGGAVIEMLARMGVNHLRIVDKDVFDYSNINRQLMSSIDHVGHPKTDVTKETIQSINPFVDIDVFNTELNESNVEEIVDGSSIVVDALDNLKTRIITSRKAMELGVPFVHGAIHGTMGQVTTFTNETPNYEETFRLNSFGKELTEDVFNEVSKLAKEVPPVIGPVPNIVGCLQSFEVVKILTGKGNIIMAPEVLIFDLLKKESFSVVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T772</accession>
    <name>F0T772_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MotA/TolQ/ExbB proton channel family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2493</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646057.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2493"/>
    <dbReference type="EnsemblBacteria" id="ADZ10706">
        <property type="protein sequence ID" value="ADZ10706"/>
        <property type="gene ID" value="Metbo_2493"/>
    </dbReference>
    <dbReference type="GeneID" id="10278967"/>
    <dbReference type="KEGG" id="mel:Metbo_2493"/>
    <dbReference type="eggNOG" id="arCOG03209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088835_0_0_2"/>
    <dbReference type="OMA" id="FIHIVSE"/>
    <dbReference type="OrthoDB" id="92251at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="94"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="24160" checksum="18B34C30D76F1B23" modified="2011-05-03" version="1">MIIFGSEILTNLLHLIAESLLIPVVIILIGFFTFAILSLGSCVTEKFSRVKFGVDKTEKLIRAISKSANPEEMKEHVRSSHLDVNMKNILIKIINNQDIDVVSRKALANKLIEELELQYSNITQKTDILVRLGPTMGLMGTLIPLGPGLAALGNGDINALAQALTISFDTTITGLAAGAIGFIISRYRKKWYMDDLSILEAIVTSTLEALERCQRKEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C1</accession>
    <name>F0T7C1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643701.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08350">
        <property type="protein sequence ID" value="ADZ08350"/>
        <property type="gene ID" value="Metbo_0097"/>
    </dbReference>
    <dbReference type="GeneID" id="10276520"/>
    <dbReference type="KEGG" id="mel:Metbo_0097"/>
    <dbReference type="eggNOG" id="arCOG06496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057818_0_0_2"/>
    <dbReference type="OMA" id="VVACKNY"/>
    <dbReference type="OrthoDB" id="104710at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Mrr_cat" evidence="2">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="265" mass="29242" checksum="1842AC314FD29F6A" modified="2011-05-03" version="1">MEKDRLVRFIAKVMEESGFKVYKDYKTSRHVIDIYGVLPTVLGDMGVVVACKNYDEHWEVGLDVLKEMEMVGKTLQASKVVVVTSSNFTTSASNYAARKNIKLVDKEGIIAIAKKFSKKDYEYEDSDVDNVEEYGDDQREVYTSIPPSKKSALFSGGNKSLSKVKSKNISPSVNLSGVVPTVKAILHNTIALIVIVLLLSSLITYLIGIFNKSTAVTGMSKILSSAILAYGLVFLVEKNSNEVLTKGTIVFFVSMIIYVVLIIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7D1</accession>
    <name>F0T7D1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643711.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0107"/>
    <dbReference type="EnsemblBacteria" id="ADZ08360">
        <property type="protein sequence ID" value="ADZ08360"/>
        <property type="gene ID" value="Metbo_0107"/>
    </dbReference>
    <dbReference type="GeneID" id="10276532"/>
    <dbReference type="KEGG" id="mel:Metbo_0107"/>
    <dbReference type="eggNOG" id="arCOG00872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002513_3_1_2"/>
    <dbReference type="OMA" id="HRAVGNY"/>
    <dbReference type="OrthoDB" id="3323at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="PTHR14025:SF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="39"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="362"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="769" mass="87311" checksum="864A28BB4BF7F664" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E0</accession>
    <name>F0T7E0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643720.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0116"/>
    <dbReference type="EnsemblBacteria" id="ADZ08369">
        <property type="protein sequence ID" value="ADZ08369"/>
        <property type="gene ID" value="Metbo_0116"/>
    </dbReference>
    <dbReference type="GeneID" id="10276541"/>
    <dbReference type="KEGG" id="mel:Metbo_0116"/>
    <dbReference type="eggNOG" id="arCOG09687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1607164_0_0_2"/>
    <dbReference type="OMA" id="TGYRENQ"/>
    <dbReference type="OrthoDB" id="108779at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="173" mass="18001" checksum="1F8D48195B9B2CF4" modified="2011-05-03" version="1">MSRLLKIILIVAFIAVFFEAGLISSYTIVTSQPPDVGKLIGMQIEEVTSLFNFGSSSSIITNPTTKPVSNLADVATALQSSSGLDGINLQSISAQVSSTTKASTFPVNITAIGYKDAISGSNSSVITITANETFSLTATANAKLSDGELVIDVDSIKITSSRKLYGNVNANST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F4</accession>
    <name>F0T7F4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643734.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0130"/>
    <dbReference type="EnsemblBacteria" id="ADZ08383">
        <property type="protein sequence ID" value="ADZ08383"/>
        <property type="gene ID" value="Metbo_0130"/>
    </dbReference>
    <dbReference type="GeneID" id="10276555"/>
    <dbReference type="KEGG" id="mel:Metbo_0130"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066901_1_0_2"/>
    <dbReference type="OMA" id="MNTWEDE"/>
    <dbReference type="OrthoDB" id="71439at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="21995" checksum="A7A33A0CE45263A7" modified="2011-05-03" version="1">MNDGKLELLTADNSVLLLVDYQPTMIRSVASGNKTIIRDAALYASKAASILNIPVVLSSINPQGNGDVFSEISEIFPDQKVFARKIKSFDAFEDDDTFNAVKSTGRKKLVVSGLWTSMCFAYTALHGIKEGYEVYGLMDAAGDSTKPAHEYGIKRMIQAGVIPMTLESLVSEWMHDWDNPKASELVKEVYSKYGAMIGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I7</accession>
    <name>F0T7I7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644906.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1313"/>
    <dbReference type="EnsemblBacteria" id="ADZ09555">
        <property type="protein sequence ID" value="ADZ09555"/>
        <property type="gene ID" value="Metbo_1313"/>
    </dbReference>
    <dbReference type="GeneID" id="10277764"/>
    <dbReference type="KEGG" id="mel:Metbo_1313"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_2"/>
    <dbReference type="OMA" id="FIMANLM"/>
    <dbReference type="OrthoDB" id="38088at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="418" mass="44680" checksum="4CE7131C4DB08EA2" modified="2011-05-03" version="1" precursor="true">MKFMSIASKDLKELLRDRRGLFFILLLPIFFMLIFSFAFGTMGQDNQPNSIAVVNLDTGVTLPNGTTVNYGNNFTDTMKGLKYQDTDVNLFNITQTSEANATNLVKQRNVDLELIIPAGFSQSMVDMAQTAQGTDSAGSMTLNSSTGNVTSKLVIRGDKGYSGFGTSLSILTGAVGSYQDGIIGAMGGVEPQEFIQTSVEGIPGTQSFTAFDYIAPGMMVFAILLLSTSVAAMLTKEVESGTLRRLKLSEMTSFDYLFGGLLPWSLVAGAQIIILLAVAIMFGFHWQGGLNSIILAAIIGVVGGVASISLGMIIASFAKNPPQASQLGTLIAVPVSFLVGAFFQLPQVVIGTFMGQEVQIYSILPWYQIATALRTVLTNGGGWDSIVYNMAAAVVLTAILFVLGVYLFSKSRLKSEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K9</accession>
    <name>F0T7K9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644928.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1336"/>
    <dbReference type="EnsemblBacteria" id="ADZ09577">
        <property type="protein sequence ID" value="ADZ09577"/>
        <property type="gene ID" value="Metbo_1336"/>
    </dbReference>
    <dbReference type="GeneID" id="10277787"/>
    <dbReference type="KEGG" id="mel:Metbo_1336"/>
    <dbReference type="eggNOG" id="arCOG00362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039376_0_0_2"/>
    <dbReference type="OMA" id="VYLFTEW"/>
    <dbReference type="OrthoDB" id="71859at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="390" mass="42826" checksum="CB1F4AD43DA9C45B" modified="2011-05-03" version="1">MKKTHIPKLDDFLGGGVPKGSSLLFSAVPGVECEAFGYQILNGFIEDGNKGFIFTNVAEPSNITYEFSRYGWDLETGIKQGDAFFIDGASSFIGMPPEGKYVINNHEDVYEVVKNAIKDVEGGVGIINNLSTLIDYNDPKEIIPLINGWNEEASKRGTVLVYLFTEWDYKANLVESIMKCFDCVVSLNSIEERVIIGQGFMVTSASWTKPQTNMVLFFVMQPGGVKIYIPKILVTGPYNAGKSSFVKAISQKSVSVDRMALEKFPTTIAMDIGHLDHGGFVADIFGTPGQERFDLMLDILAKEAVGAFILVDSSAPQTFARAKEMINKTHSEAIPNIIVANKQDLEGALSPEQIREKMNLDEKIPILPTVVTTKTGVDQALNTLLKLLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7M7</accession>
    <name>F0T7M7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09595">
        <property type="protein sequence ID" value="ADZ09595"/>
        <property type="gene ID" value="Metbo_1355"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1355"/>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029015_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018975">
        <property type="entry name" value="Pseudomurein-binding_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09373">
        <property type="entry name" value="PMBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="29365" checksum="F1ABA8012E723FF0" modified="2011-05-03" version="1" precursor="true">MGNGGIAIRKKLLYSITALLFLVLILNIGAGAAASTAQASGSTTGTIKTTTTSNTTLVSGLTSAQIKDGYTRAQNFYNTNHRLPNYVSYGTKKVMISDFQTILATQGLKINMYYSRPVYITSDNINNKTADNTRINNIVTGLKALGIKAYNMGIGPNTHVKVLQNTNVPQNALIVDIYGGADAGTLLEMGQSWYKKIKGARSVYSIFWPPSKVITGLAFLERAHDDNYSPASFTGLANPDQYLINNGYNYIYSKDIKTIVNSIFKQVSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S9</accession>
    <name>F0T7S9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="4">1.8.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0164</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643767.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0164"/>
    <dbReference type="EnsemblBacteria" id="ADZ08416">
        <property type="protein sequence ID" value="ADZ08416"/>
        <property type="gene ID" value="Metbo_0164"/>
    </dbReference>
    <dbReference type="GeneID" id="10276590"/>
    <dbReference type="KEGG" id="mel:Metbo_0164"/>
    <dbReference type="eggNOG" id="arCOG02235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="TAKHAML"/>
    <dbReference type="OrthoDB" id="1148at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="245"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="583"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="612"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="658" mass="71689" checksum="C40BB1DF2288EEED" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T7</accession>
    <name>F0T7T7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0172</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643775.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0172"/>
    <dbReference type="EnsemblBacteria" id="ADZ08424">
        <property type="protein sequence ID" value="ADZ08424"/>
        <property type="gene ID" value="Metbo_0172"/>
    </dbReference>
    <dbReference type="GeneID" id="10276598"/>
    <dbReference type="KEGG" id="mel:Metbo_0172"/>
    <dbReference type="eggNOG" id="arCOG00065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_4_2"/>
    <dbReference type="OMA" id="PVCLRYG"/>
    <dbReference type="OrthoDB" id="24071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="20"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="402" mass="43938" checksum="E74E7C8E417AEA19" modified="2011-05-03" version="1">MENGVDINIRADIPLLEDVIYLDAASTTPTPKPVVESMCNYFYNYNTNTGRGAYNLAVRATKEFESSRKRISKFVGSKPSEIIFTKNTTEAINLVANGLNFKKGDSVVVPNIEHHSNFVPWLNLRKKGVNLKLVEADEYGIIDHSDVLDAVDANTKLITTTHVSNAIGSVQPIKEIGEIADENDVLYLVDAAQSAGHMPLDVKDIKADFVSVPGHKGLLGPIGTGFLYCNQEISEDLEPTNYGGGTVLDVTESDFTLETVPARFEGGTQNIAGVIGLGTAVDYLERIGMEKIQNHSKKLTAKLFKEVNDIENTIVYGSPENIYGIVAFNIDGVNAHDVAKILDEVKGICVRSGHHCAIPAIRHMGAYELGGTVRASVHYYNTVEEIEIFGETLREISKFFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7U2</accession>
    <name>F0T7U2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="2">N5-CAIR mutase</shortName>
            <ecNumber evidence="2">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purE</name>
        <name evidence="4" type="ordered locus">Metbo_0177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643780.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0177"/>
    <dbReference type="EnsemblBacteria" id="ADZ08429">
        <property type="protein sequence ID" value="ADZ08429"/>
        <property type="gene ID" value="Metbo_0177"/>
    </dbReference>
    <dbReference type="GeneID" id="10276603"/>
    <dbReference type="KEGG" id="mel:Metbo_0177"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782152_0_0_2"/>
    <dbReference type="OMA" id="AHRTHEK"/>
    <dbReference type="OrthoDB" id="48892at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="3">
        <location>
            <begin position="3"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="AIRC" evidence="3">
        <location>
            <begin position="194"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="340" mass="36696" checksum="EF1749D692698E1C" modified="2011-05-03" version="1">MEPKIMIILGSASDFDVAEKAINILEILEISYDVRVASAHRTHEKVKKIVEESTAHGVEVFIGIAGLSAHLPGIIAANTYKPVVGVPVSVKLGGLDALFATVQMPVGAPVATVGIDRGENGAILASQIIGIHDSEVRTRLSKMRNEFFFKIKNDEDKLAEKLTGTYYNRHSFEIDELEGSVRTDEDKYSDPEYPEVAVIAGSYSDMKIANKTTKFLDKLGVKYDSAVISPIRHPKKFEEYMEKSSSVKLYIAISGLSAHVTGAVVAFTEKPVLGVPCAIRLDGMDALLSMVNMPPGVPVATLGIDSGSNAALLAAEMLGLADASIRNSLKNFKDNMNCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7X4</accession>
    <name>F0T7X4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2a</name>
        <name evidence="11" type="ordered locus">Metbo_0209</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643812.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0209"/>
    <dbReference type="EnsemblBacteria" id="ADZ08461">
        <property type="protein sequence ID" value="ADZ08461"/>
        <property type="gene ID" value="Metbo_0209"/>
    </dbReference>
    <dbReference type="GeneID" id="10276635"/>
    <dbReference type="KEGG" id="mel:Metbo_0209"/>
    <dbReference type="eggNOG" id="arCOG04107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="OrthoDB" id="84684at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="PTHR10602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="SSF110993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="SSF116742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="259" mass="29167" checksum="30336DF2A4906DFD" modified="2011-05-03" version="1">MVRMRKEWPDEGDLVVGTVHKVLNYGAFGSLEEYDGKEAFIHISEVSSGWVKNIRDYVRENQKIVARVLRVNPKKGHVDVSLKRIREDQRTRKIQQWKIEQKAEKLLEFSAKSLEKTLDDAYKEVGYGIMEEFGDLYGAFETAAEEGAISLIDRGMDEDWANSIEAVAKKNISPPEVNITGYVDLKSYAPNGVDVIKKALSSIDSDEISVQCVGAPRYRLMVKSSDYITAETLLKEAAENAIKVVIEEGGEGTFQRELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T815</accession>
    <name>F0T815_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644992.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1401"/>
    <dbReference type="EnsemblBacteria" id="ADZ09641">
        <property type="protein sequence ID" value="ADZ09641"/>
        <property type="gene ID" value="Metbo_1401"/>
    </dbReference>
    <dbReference type="GeneID" id="10277852"/>
    <dbReference type="KEGG" id="mel:Metbo_1401"/>
    <dbReference type="eggNOG" id="arCOG00850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_3_0_2"/>
    <dbReference type="OMA" id="TRCHIGD"/>
    <dbReference type="OrthoDB" id="58158at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="168" mass="19073" checksum="08F83068895FEC33" modified="2011-05-03" version="1">MIIRNEKIEDHEAVREINLKAFPTDIEATLVDKLRSSMDTISLVAVHEDKVVGHILFSPLTIENDEESFPALILAPIAVLPEYQKQGIGSKLVENGIIECRNHGHSIIILVGHPGYYPRFGFISAEQNRIEHPFEVPEDVFMVYELVPDTLSRVNGVLKYSKPFEEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T843</accession>
    <name>F0T843_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyridoxamine 5'-phosphate oxidase-related FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645020.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1431"/>
    <dbReference type="EnsemblBacteria" id="ADZ09669">
        <property type="protein sequence ID" value="ADZ09669"/>
        <property type="gene ID" value="Metbo_1431"/>
    </dbReference>
    <dbReference type="GeneID" id="10277882"/>
    <dbReference type="KEGG" id="mel:Metbo_1431"/>
    <dbReference type="eggNOG" id="arCOG00525">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884589_0_0_2"/>
    <dbReference type="OMA" id="RATPLEY"/>
    <dbReference type="OrthoDB" id="53028at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026816">
        <property type="entry name" value="Flavodoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12724">
        <property type="entry name" value="Flavodoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin_5" evidence="2">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Putative_PNPOx" evidence="1">
        <location>
            <begin position="178"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="314" mass="35944" checksum="144DC7E079C68990" modified="2011-05-03" version="1">MLKTLVVYSGKYNTTEEVAKTISLITGPSRYCTVDNFKEEYRDFDFVVLGAPIYEEKVDPAMVEFVKENSTWLAKKAVAAYCTCLDTNGGLRELNNLAFDYQINFLTLKALGGRLILDKLDSEDKVAIEEFLELVKLPHQDMDFYNEEEVIKFALKLKELKEELLSKLDDEKLLVMVEEFINSHNTCCLATCHNGTVRSTPIEYNYRNKNLYLLSEGGEKFSNLLLNENVSVSIYDSFKGMNSLAGMQITGKATIIAETSEEYEEVIEMKGLNLESLKRLPVNINLIKIQVDRIEFLFSKFKEMNADTRQIVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T846</accession>
    <name>F0T846_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein TetR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645023.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1434"/>
    <dbReference type="EnsemblBacteria" id="ADZ09672">
        <property type="protein sequence ID" value="ADZ09672"/>
        <property type="gene ID" value="Metbo_1434"/>
    </dbReference>
    <dbReference type="GeneID" id="10277885"/>
    <dbReference type="KEGG" id="mel:Metbo_1434"/>
    <dbReference type="eggNOG" id="arCOG02648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_42_0_2"/>
    <dbReference type="OMA" id="KILAEWN"/>
    <dbReference type="OrthoDB" id="81806at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="23956" checksum="7D4CA2E73E71FBA4" modified="2011-05-03" version="1">MPKKFGENEKQIIVKKLKETGVELFSKYGLKKTSIYDLTSEAEIAQGSFYNFFDSKEELYFSLLESEEEEMENHMKKIILNSGSAREAIKITIVESCSLFEKTPLLRRIYESNDYELMVRKLPHEKLLKHQKNDTKLVINTILKVKTDDEEITADPEIISGILRSITLLNLHKKEIGRDIYPEIIEILAESIADGLVRKTGQKTTPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T851</accession>
    <name>F0T851_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645028.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1439"/>
    <dbReference type="EnsemblBacteria" id="ADZ09677">
        <property type="protein sequence ID" value="ADZ09677"/>
        <property type="gene ID" value="Metbo_1439"/>
    </dbReference>
    <dbReference type="GeneID" id="10277890"/>
    <dbReference type="KEGG" id="mel:Metbo_1439"/>
    <dbReference type="eggNOG" id="arCOG02552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716911_0_0_2"/>
    <dbReference type="OMA" id="CTIANNI"/>
    <dbReference type="OrthoDB" id="31159at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="93"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Beta_helix" evidence="1">
        <location>
            <begin position="250"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="385" mass="41473" checksum="CA54CC5376F47592" modified="2011-05-03" version="1" precursor="true">MKKKMILCTLFLCLCMISINSISAANVTVNPGSSIQNAVNKANPGDTIIINDNNKQPYTYKESITLNKKLNIISNGSVTIKAKTTNSAIFTIYSKAAGSSIKNFKLSDTNYSIMVSGASYCTISGNTITSASLVGIQFYGDITKSQVLNNKISGTTTKWGNGISFEYGKSTYNTIKGNTISNYLNGILFNDCSAYNTVSNNKVRCYALKGAGIYFTDNAKYNKIIYNTVTGAEDGIAIQKMGSGMASHFTITGNTVKYNKNGFWIRLTSSTISYNTANYNKVSGIDVTGSYNKIVHNTATHNGVCGIALGRYSSCDHNTVQYNILSYNKAGINSASNYTTISSNTVQHNTSNGIISTANHVTIKNNSVNYSANRILVQGSSNKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T865</accession>
    <name>F0T865_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645042.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1454"/>
    <dbReference type="EnsemblBacteria" id="ADZ09691">
        <property type="protein sequence ID" value="ADZ09691"/>
        <property type="gene ID" value="Metbo_1454"/>
    </dbReference>
    <dbReference type="GeneID" id="10277905"/>
    <dbReference type="KEGG" id="mel:Metbo_1454"/>
    <dbReference type="eggNOG" id="arCOG01471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237929_0_0_2"/>
    <dbReference type="OrthoDB" id="134258at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="1">
        <location>
            <begin position="38"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="223" mass="26066" checksum="20869CAE62E75242" modified="2011-05-03" version="1">MSNENQPDLGEDYIRLHKVMTRGIEVSKHSIDKYMNEEAMDELNREGFTNYIQSFSAVLHGHHMVEDQKIFPYFKDQLPEVPYPRLTSEHEIFNQGLQEINTATKELTAKNNELESLNLLKSGLNKVDKLWDAHIQIENTQLYGKIRSLDMDPEEMNKIGNESKEFFQAHSGPPYLVIPFVLYNLSLDDRKIISQSFPDEVINQLLFGDWKDKWTSMQPYLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D7</accession>
    <name>F0T8D7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643900.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0297"/>
    <dbReference type="EnsemblBacteria" id="ADZ08549">
        <property type="protein sequence ID" value="ADZ08549"/>
        <property type="gene ID" value="Metbo_0297"/>
    </dbReference>
    <dbReference type="GeneID" id="10276729"/>
    <dbReference type="KEGG" id="mel:Metbo_0297"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_2_1_2"/>
    <dbReference type="OMA" id="VHRHHTI"/>
    <dbReference type="OrthoDB" id="67420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="78"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="141"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="230"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="30991" checksum="FD93C59BC58463CA" modified="2011-05-03" version="1">MEISELMNPEVFVIQENQHVSQARNLMISHGISRVVVVDGNGAPVGMVTEKDLTKKLKGKGPRWKTRPLDKISIKRVMSSNPITASPDDNVQKVIELLIKNHIGSVPIVDEDGLAGIITKTDLMKIYTDKLRGKWKVSDLMTGDVITVNENHSIAHVISLMEDNRIGKMIVIRDNEPVGIITHEHISFAYIEDPETGVNVEKIYFIRNSEDGQSKKNFRVVSMMTAGDIMQNHMIKISLDEDAATAADMMIENDINGLAVVNGDVLVGVITKTDLIKGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8D9</accession>
    <name>F0T8D9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Y414 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0299"/>
    <dbReference type="EnsemblBacteria" id="ADZ08551">
        <property type="protein sequence ID" value="ADZ08551"/>
        <property type="gene ID" value="Metbo_0299"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0299"/>
    <dbReference type="eggNOG" id="arCOG04218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061502_0_0_2"/>
    <dbReference type="OMA" id="ICPFTTK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01209">
        <property type="entry name" value="TIGR01209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_ligase" evidence="1">
        <location>
            <begin position="115"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Lig_C" evidence="2">
        <location>
            <begin position="282"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="404" mass="46372" checksum="AB785F6830691FCE" modified="2011-05-03" version="1">MSVKVGKSKSHKIFFLSCDECGQEIEVSMPEFIIGILPKMLDLKSENLERSFKNGNIKFYSGQGVEGLQFRKDVGHIESGTMICFGNKIEIVRGFPKIRRTLLLNPSLVNHFKTEVAVEEKMNGYNVRLALISQKIKAFTRGGYICPYTTKKAPEILDMEDFFNDNPELVICGEMVGTENPYVTHHYREIGKLGFRIFDLRYKTTNEPLNIKEKYQLLDSYNLPSVKSYGIIPIEEAVERIFDIIQVMGQEDREGVVIKDPEMELEPVKYTTSRAHLGEIEYAFNYPFDFGRSFFFSRVIREGFQAFELDESEEEIRKRAHLLGESILFPMVNTIKHVSGDNSAFEDIVIHVENNTDAEEFARHLRDLGVVAIVQSFDDGKAVIRRIHQSTTDRISNYLRGGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8G8</accession>
    <name>F0T8G8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645070.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09719">
        <property type="protein sequence ID" value="ADZ09719"/>
        <property type="gene ID" value="Metbo_1486"/>
    </dbReference>
    <dbReference type="GeneID" id="10277937"/>
    <dbReference type="KEGG" id="mel:Metbo_1486"/>
    <dbReference type="HOGENOM" id="CLU_2366216_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10697" checksum="7EB39AF56A66E0EC" modified="2011-05-03" version="1">MDGLLQMLSVKVDMIVSAGSVLTLLALIIIYLKNYKTFKTKFSLAILLFVSLFFMEELIYTVSVFNDPSISISLQTLINIIELIGFLIILSVARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I8</accession>
    <name>F0T8I8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645090.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09739">
        <property type="protein sequence ID" value="ADZ09739"/>
        <property type="gene ID" value="Metbo_1508"/>
    </dbReference>
    <dbReference type="GeneID" id="10277959"/>
    <dbReference type="KEGG" id="mel:Metbo_1508"/>
    <dbReference type="eggNOG" id="arCOG03293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140793_0_0_2"/>
    <dbReference type="OMA" id="DKVNGRF"/>
    <dbReference type="OrthoDB" id="104892at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="123" mass="13994" checksum="2917E0F0AC094299" modified="2011-05-03" version="1">MGILSSNEKTPEEKRIEDLMRKPINGAIKPHLKAALKKMASEGKSIDEIERHYNATVTKTQFIENRKAEYATEAKLADQVYKAYITDGLSRLDKVNGRFLASISMKNDILIEQNNRIIRNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K7</accession>
    <name>F0T8K7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tetratricopeptide TPR_1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">AL-21</strain>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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        </source>
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        <source>
            <dbReference type="EMBL" id="ADZ08553.1"/>
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    </evidence>
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        <source>
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    <sequence length="194" mass="21283" checksum="743F8291A65EA2F6" modified="2011-05-03" version="1">MERISIVSDKDRLLKSLKKLETQFANGKVSKRQYSREKRVLEDKLSTILAADRIKRLQGKTVDETPLESEILKEKEDTVEKEELIKKYVTTPKKVPVEPKSSGMSKGKVALIVFLVAAFFVGTGYGVYVMSMPSNNSSVTMTVNDSAFPVINNTTNTTANKTITTNKTTKTTSTTKKNSSSTTGTTTKTGNSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U7</accession>
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Detter J.C."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="2 3" key="2">
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                <person name="Brauer S.L."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="61"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="97"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="12854" checksum="5E1A4DB95D0AE7AF" modified="2011-05-03" version="1">MVVGTKYCSNCGQQIDEEAEVCPECGVRQARPVYREVVYQQKNPGLAAVLSALFVGFGQIYNGQIAKGLIMMVLYFFSILLVLVLIGLITTPLIWIFGIYDAYNTAKRINDGEIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X8</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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    </dbReference>
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        <property type="protein sequence ID" value="ADZ09806"/>
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    </dbReference>
    <dbReference type="GeneID" id="10278035"/>
    <dbReference type="KEGG" id="mel:Metbo_1579"/>
    <dbReference type="HOGENOM" id="CLU_1870750_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="15691" checksum="0487EA5C16A03D96" modified="2011-05-03" version="1" precursor="true">MYTKLAIFASISLCVFSLLVLPVFAEDFNEFRITGPHSEDHYLNMFGVMRGDPINIELKLSRNIGFWNPQGFQWINTTIYDVHLNYVCSQYTQTDEWKGVTKLQTFTINTNGTYILVANYTNNTHHCFAATAFFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y8</accession>
    <name>F0T8Y8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645167.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1589"/>
    <dbReference type="EnsemblBacteria" id="ADZ09816">
        <property type="protein sequence ID" value="ADZ09816"/>
        <property type="gene ID" value="Metbo_1589"/>
    </dbReference>
    <dbReference type="GeneID" id="10278046"/>
    <dbReference type="KEGG" id="mel:Metbo_1589"/>
    <dbReference type="eggNOG" id="arCOG02644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_1_2"/>
    <dbReference type="OMA" id="QKADEAM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="211" mass="24762" checksum="7825CBB7EABACC1C" modified="2011-05-03" version="1">MTKAERKQRELELRRNDIVDAAEKLFFAKGYETVNMNDIAQEAEMARGTLYQYFKNKEDIYASIALRAAKMINERFQELLSHDLNGIDKVRWVCKFYYDFYREYPGYYDAYYHSQMFEIDDSKTLKKLQKIRKTSFKLAVNSIKEGIEDGTLRDDIDPVVTALYLLSTSNNINNISPVTQMYLDEYGLTHDDLFNQTLDISIRSIENSKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z9</accession>
    <name>F0T8Z9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intimin/invasin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645178.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1600"/>
    <dbReference type="EnsemblBacteria" id="ADZ09827">
        <property type="protein sequence ID" value="ADZ09827"/>
        <property type="gene ID" value="Metbo_1600"/>
    </dbReference>
    <dbReference type="GeneID" id="10278057"/>
    <dbReference type="KEGG" id="mel:Metbo_1600"/>
    <dbReference type="eggNOG" id="arCOG02487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_313006_0_0_2"/>
    <dbReference type="OMA" id="SVINCTF"/>
    <dbReference type="OrthoDB" id="495at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003344">
        <property type="entry name" value="Big_1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032109">
        <property type="entry name" value="Big_3_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02369">
        <property type="entry name" value="Big_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16640">
        <property type="entry name" value="Big_3_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00634">
        <property type="entry name" value="BID_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="SSF49447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51127">
        <property type="entry name" value="BIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="Big-1" evidence="3">
        <location>
            <begin position="598"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Big-1" evidence="3">
        <location>
            <begin position="693"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1150" mass="121229" checksum="C9759993B21AC3A2" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T943</accession>
    <name>F0T943_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Type II secretion system F domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0413</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644016.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0413"/>
    <dbReference type="EnsemblBacteria" id="ADZ08665">
        <property type="protein sequence ID" value="ADZ08665"/>
        <property type="gene ID" value="Metbo_0413"/>
    </dbReference>
    <dbReference type="GeneID" id="10276850"/>
    <dbReference type="KEGG" id="mel:Metbo_0413"/>
    <dbReference type="eggNOG" id="arCOG01811">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077875_0_0_2"/>
    <dbReference type="OMA" id="ITEYRLW"/>
    <dbReference type="OrthoDB" id="89137at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        <location>
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            <end position="231"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="310" mass="33738" checksum="93BB9885C238E01F" modified="2011-05-03" version="1">MAIIPPALSPVSRILDDVLPERYTVFMQENLIRSGMYVKASDLITLMLIAGIIFGLIGLLSFSLIGITPILGFLVGFLTPSFIVLGWLFFSMERRVDAIEQTTPDFLRQISSLLRAGVGIETALEDISKQGGGPLNAELKRAVIEIKIGSSFDEALLSMGERLKSKTLDRTFRMILEGKRVGGSLSDVIETVAEDLRAVLALKRERRANVMMSVMFLIISAIIAAPFALGMIMIYSHFIQSLGKVNPLAEVAYTSAAGYLIIHSIIVGVLIGIVLYGSARKGVKFSLALVPLAYVIFYVISTYGGLLIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T961</accession>
    <name>F0T961_METLA</name>
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    </protein>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644034.1">
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0431"/>
    <dbReference type="EnsemblBacteria" id="ADZ08683">
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        <property type="gene ID" value="Metbo_0431"/>
    </dbReference>
    <dbReference type="GeneID" id="10276868"/>
    <dbReference type="KEGG" id="mel:Metbo_0431"/>
    <dbReference type="eggNOG" id="arCOG03363">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160668_3_0_2"/>
    <dbReference type="OMA" id="KIMEDSM"/>
    <dbReference type="OrthoDB" id="129046at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014275">
        <property type="entry name" value="ATPase_A1A0-cplx_hsu"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02926">
        <property type="entry name" value="AhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="11423" checksum="74235914A92C63DA" modified="2011-05-03" version="1">MTTISEAITTIKKAENDADKLIEDTNELKSERIEEARSKSKEIIAKSKEEASAEAENMAVEAETKAKKEALHISNTTSEEVRVTKSTAMNKVDEAADLIVKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T992</accession>
    <name>F0T992_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved repeat domain protein</fullName>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09843">
        <property type="protein sequence ID" value="ADZ09843"/>
        <property type="gene ID" value="Metbo_1616"/>
    </dbReference>
    <dbReference type="GeneID" id="10278073"/>
    <dbReference type="KEGG" id="mel:Metbo_1616"/>
    <dbReference type="eggNOG" id="arCOG07611">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592532_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DUF11"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="563"/>
        </location>
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        <location>
            <begin position="58"/>
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        </location>
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        </location>
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        <location>
            <begin position="409"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="567" mass="61908" checksum="5361945B16178A49" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A3</accession>
    <name>F0T9A3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1627</name>
    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645205.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09854">
        <property type="protein sequence ID" value="ADZ09854"/>
        <property type="gene ID" value="Metbo_1627"/>
    </dbReference>
    <dbReference type="GeneID" id="10278084"/>
    <dbReference type="KEGG" id="mel:Metbo_1627"/>
    <dbReference type="eggNOG" id="arCOG02879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079270_3_0_2"/>
    <dbReference type="OMA" id="RYPFSDW"/>
    <dbReference type="OrthoDB" id="88529at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="270" mass="30698" checksum="5F42864812335563" modified="2011-05-03" version="1">MSIGEITKDALRYPFSDWKKILVLGIFGVIATLSINELIGTVYLFFDSSASVSIVVLTIIGLLSIFIERGYQFRILKSSLNGMVELPKFNSWMSMLKDGFKLSIVTIVYLIPFVLIYLVLKGPFVAILQLIFKSPIKMSFFTTVGILHLMEFFILFLFINIILIMFMISIANMANNNGKLSAAFKFRQITNKLSSVGWKNLIVWYVLTEFIYLIILFISGIIIKEISHLIIPSAGNALGPLIITILMSLIVISYLYMYLYRSLALLWLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A8</accession>
    <name>F0T9A8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pseudomurein-binding repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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    <sequence length="315" mass="34466" checksum="716F4F0A4023F838" modified="2011-05-03" version="1" precursor="true">MGGGIIKRNWILPVLMLVGMVLIFNVSGVSAATTSSSTTINHTKTVTAKQTAVKINFTPSQINTAASKVKTYYETNHKLPCYVNINNHKVTMPQFLQLVTDNIYQLSNKKSNSITLKNVTAPSKPVENVKTGQLTQAQYISLAKTTRNYIYTKGAAPNNVNSSLGTIKFQNLVYSFSKVLAFQATNNRLPNYVSVTSLTQISTSTVKNGSNAQAIVDSIGYAEAKFEDIQGQSSPCVMDQCGYGDCWADSGWLYNKLSKAGIAVRVIGHTKGSYPLHRWIEINIGNGWKTWDYAKYNSQHHGPTGSGDYVVKSSV</sequence>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
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                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
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    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50850"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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        <source>
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</entry>
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    <accession>F0T9F2</accession>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="EMBL" id="ADZ09903.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9L5</accession>
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                <person name="Lapidus A."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
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    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
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        <property type="entry name" value="Usp"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15469" checksum="9BA179D04713AD6E" modified="2011-05-03" version="1">MFQKILVPINPIEDQDLLVKTAYNLGIERDSEVKFVYFGDDPKAISQLEFYIEKCTQKGLNASYYFEKFVGTKEEIPEKIAKISEEYDLVIMGHLKYDKIYRFVHQSTASDLINLVTIPVMIIPDSGECHLILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q6</accession>
    <name>F0T9Q6_METLA</name>
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            <fullName evidence="2">YhgE/Pip N-terminal domain protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriaceae</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645286.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1711"/>
    <dbReference type="EnsemblBacteria" id="ADZ09935">
        <property type="protein sequence ID" value="ADZ09935"/>
        <property type="gene ID" value="Metbo_1711"/>
    </dbReference>
    <dbReference type="GeneID" id="10278168"/>
    <dbReference type="KEGG" id="mel:Metbo_1711"/>
    <dbReference type="eggNOG" id="arCOG06504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004534_2_0_2"/>
    <dbReference type="OMA" id="YLWSFWD"/>
    <dbReference type="OrthoDB" id="15069at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017501">
        <property type="entry name" value="Phage_infect_YhgE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017500">
        <property type="entry name" value="Phage_infect_YhgE_N"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03062">
        <property type="entry name" value="pip_yhgE_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03061">
        <property type="entry name" value="pip_yhgE_Nterm"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="634" mass="69433" checksum="38ED19D5746BBC9E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T0</accession>
    <name>F0T9T0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_1735</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645310.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09959">
        <property type="protein sequence ID" value="ADZ09959"/>
        <property type="gene ID" value="Metbo_1735"/>
    </dbReference>
    <dbReference type="GeneID" id="10278192"/>
    <dbReference type="KEGG" id="mel:Metbo_1735"/>
    <dbReference type="eggNOG" id="arCOG01964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_6_1_2"/>
    <dbReference type="OMA" id="FYYMEVN"/>
    <dbReference type="OrthoDB" id="111041at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="10">
        <location>
            <begin position="163"/>
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        </location>
    </feature>
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            <begin position="241"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="31485" checksum="AAAC6D0746B6F97C" modified="2011-05-03" version="1">MGKAGAVGDKKILGKKVGSTGAIEEPMIQRRDLLNKLSNVKDVIIILWMLADIILLTLTYFVDLSNIYLFIVVFDTGLCAVLIVEFSYNLYQKENKLAYLREDWKGVIVDILAMFPYELITFGSYGFIRLLRLVRIFALFGKGRRNIFNFVEKTHLNYILFTLILIVAASTIAVLVLESSPTGEIHTPLDALWYVVSTVTTVGYGDIVPVSPGGKALGIILMIVGVGFFSLLTATLSSYFMRDMESEEKELKEQVESLENSINEMKSEIAEFKELLKKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T4</accession>
    <name>F0T9T4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09963">
        <property type="protein sequence ID" value="ADZ09963"/>
        <property type="gene ID" value="Metbo_1739"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1739"/>
    <dbReference type="HOGENOM" id="CLU_2695801_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="73" mass="8689" checksum="F83409B9C8DA1AEA" modified="2011-05-03" version="1">MLFKNNESPNKISECQFTIKQLCRNNWDDVILILSSTFRYMPLNIIFYFEPLYSCLEYPIEVGINLKLDSTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9X7</accession>
    <name>F0T9X7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="4">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ftsY</name>
        <name evidence="7" type="ordered locus">Metbo_0548</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644151.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0548"/>
    <dbReference type="EnsemblBacteria" id="ADZ08800">
        <property type="protein sequence ID" value="ADZ08800"/>
        <property type="gene ID" value="Metbo_0548"/>
    </dbReference>
    <dbReference type="GeneID" id="10276993"/>
    <dbReference type="KEGG" id="mel:Metbo_0548"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="OrthoDB" id="90641at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="238"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="320"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="378"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="427" mass="46399" checksum="8FA6A6601359B4A4" modified="2011-05-03" version="1">MFESLKKKFKGTIGKISDNVASEEELDDSNSSAADIKSKDETVPSSDSSENKSSKAVSKNDLTESSKDVSETESSETEAESDSKKSGFMGFLRGNKSSEKEESEDSKPSKTSETEEISADETITESEEKPEEDDKSGILSFVTNKTISEKDIEDILFELEMSLLEGDVAMEVAETIITSVKTDLVGQKIRRRSDVAEFTREALKKAISEILDIPGKDLTSLLEEAKKSGEPLKIMFVGINGTGKTTTIAKISTYFINKGYTPVIAASDTFRAGAIEQLTHHADNLDVKIIKHKKGADPAAVAFDAVAHARAKGKELVLVDTAGRMQTNINLMDEMKKIKRVIKPDLIIYVGDALTGNDSVEQAKKFNDAVTVDGIILTKADADAKGGAALSIGYVIQKPILFLGVGQSYSDIIEFKPEWMVEQILGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA34</accession>
    <name>F0TA34_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Potassium-transporting ATPase potassium-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP phosphohydrolase [potassium-transporting] A chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Potassium-binding and translocating subunit A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Potassium-translocating ATPase A chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">kdpA</name>
        <name evidence="10" type="ordered locus">Metbo_1761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit binds the extracellular potassium ions and delivers the ions to the membrane domain of KdpB through an intramembrane tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KdpA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1761"/>
    <dbReference type="EnsemblBacteria" id="ADZ09984">
        <property type="protein sequence ID" value="ADZ09984"/>
        <property type="gene ID" value="Metbo_1761"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1761"/>
    <dbReference type="eggNOG" id="arCOG04804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018614_3_0_2"/>
    <dbReference type="OMA" id="RQGWAIL"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00275">
        <property type="entry name" value="KdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004623">
        <property type="entry name" value="KdpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30607">
        <property type="entry name" value="PTHR30607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03814">
        <property type="entry name" value="KdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001294">
        <property type="entry name" value="K_ATPaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00680">
        <property type="entry name" value="kdpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="567" mass="60709" checksum="2B7CEB4336A7C451" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA70</accession>
    <name>F0TA70_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Propeptide PepSY amd peptidase M4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645371.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1798"/>
    <dbReference type="EnsemblBacteria" id="ADZ10020">
        <property type="protein sequence ID" value="ADZ10020"/>
        <property type="gene ID" value="Metbo_1798"/>
    </dbReference>
    <dbReference type="GeneID" id="25394747"/>
    <dbReference type="KEGG" id="mel:Metbo_1798"/>
    <dbReference type="eggNOG" id="arCOG09730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143945_0_0_2"/>
    <dbReference type="OrthoDB" id="121896at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="12812" checksum="7F0CD8B7A60AB249" modified="2011-05-03" version="1" precursor="true">MKKAIAVTLVVVVAIIGVAMALNSTANSKNSTNNSTSSDTAKNVDVGNTSTNNNQSVDNMISSSEAQKKASEYINQTGAYAGTPKLVNQDGTMVYIVPVIYNGQTAGEIDIDAQTGRNLGGAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA80</accession>
    <name>F0TA80_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="6" type="ordered locus">Metbo_1808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645381.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1808"/>
    <dbReference type="EnsemblBacteria" id="ADZ10030">
        <property type="protein sequence ID" value="ADZ10030"/>
        <property type="gene ID" value="Metbo_1808"/>
    </dbReference>
    <dbReference type="GeneID" id="10278265"/>
    <dbReference type="KEGG" id="mel:Metbo_1808"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="OMA" id="FENDFLC"/>
    <dbReference type="OrthoDB" id="75288at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00373">
        <property type="entry name" value="TIGR00373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="19431" checksum="295FA1FE3CD0FA7D" modified="2011-05-03" version="1">MFRDPLVKEILMDITNDGESSVPIIECIINGKTFDEEIAEETDIRLNTVRKILYKLHDAGVANYKRSRDPETNWYIYSWKFEEDKISEIISKKFEKYSEDIEKSLEYEEGNMFFICKANGHRYLFEEASENNFVCPECQNTLEYQDNSTIIQELKHMLKTNQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA92</accession>
    <name>F0TA92_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645393.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1820"/>
    <dbReference type="EnsemblBacteria" id="ADZ10042">
        <property type="protein sequence ID" value="ADZ10042"/>
        <property type="gene ID" value="Metbo_1820"/>
    </dbReference>
    <dbReference type="GeneID" id="10278277"/>
    <dbReference type="KEGG" id="mel:Metbo_1820"/>
    <dbReference type="eggNOG" id="arCOG10243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911531_0_0_2"/>
    <dbReference type="OrthoDB" id="131893at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="61" mass="6936" checksum="5F24E44909C125BC" modified="2011-05-03" version="1">MKPIVKGIKNYFSDKKNRIVHVIAGISGLLASLFSLMGFYDRILLFAVAICFNVVRMRMMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAB3</accession>
    <name>F0TAB3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644216.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0614"/>
    <dbReference type="EnsemblBacteria" id="ADZ08865">
        <property type="protein sequence ID" value="ADZ08865"/>
        <property type="gene ID" value="Metbo_0614"/>
    </dbReference>
    <dbReference type="GeneID" id="10277059"/>
    <dbReference type="KEGG" id="mel:Metbo_0614"/>
    <dbReference type="eggNOG" id="arCOG01994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099320_0_1_2"/>
    <dbReference type="OMA" id="THIVIRC"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="210" mass="23118" checksum="5CB4E404AC8ACB9C" modified="2011-05-03" version="1" precursor="true">MKKERYDGFFGGLYKRNEVFIMLSAVILLGSIFAGYAFAGVLNSVLGGFYGDFQNKIIHGQIQLNTFSLFFNNLNVALLIYIGGAFVGIISAGYLIFNGLFIGYAGTQFPLGDYLVYTLPHGIPELLGIIIAGAAGFRLGSCVLNIFKDLLHMRTDISKGTQFNYIIGMHIDEFWESLKLLFIAAVLLIIAAFIEANLTIAWGNYVKSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAE2</accession>
    <name>F0TAE2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase C60 sortase A and B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644245.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0643"/>
    <dbReference type="EnsemblBacteria" id="ADZ08894">
        <property type="protein sequence ID" value="ADZ08894"/>
        <property type="gene ID" value="Metbo_0643"/>
    </dbReference>
    <dbReference type="GeneID" id="10277088"/>
    <dbReference type="KEGG" id="mel:Metbo_0643"/>
    <dbReference type="eggNOG" id="arCOG07610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352136_0_0_2"/>
    <dbReference type="OMA" id="VYHYPES"/>
    <dbReference type="OrthoDB" id="80857at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="21570" checksum="EEE711D08A267046" modified="2011-05-03" version="1" precursor="true">MKRTNWIALGIVAVCIVVSLTIALAAFEQSKNLTAAQKSLKDYNEKMSQPVDALDPLSTKVGASLGELVIPKLGVNCTIRSDTVNAYNAVYHYPESAMPGQPGECGILGHRTTYSGLFENIGSLEVGDQVIIVDTIMKKKYTYAVTSNGNDIRWDYKTNPVTFALDGKARLMLMTCYPPGKKEAAYIAHCELVSTDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY0</accession>
    <name>F0TAY0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0755"/>
    <dbReference type="EnsemblBacteria" id="ADZ09006">
        <property type="protein sequence ID" value="ADZ09006"/>
        <property type="gene ID" value="Metbo_0755"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0755"/>
    <dbReference type="eggNOG" id="arCOG12083">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465115_0_0_2"/>
    <dbReference type="OMA" id="KHFMIFN"/>
    <dbReference type="OrthoDB" id="91013at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="197" mass="23174" checksum="E8C5EE7DF25670EB" modified="2011-05-03" version="1">MAIISISTTLVVILLIRVIYRHNPHVHKLYGREIVLFHSSQRYEKRIWRFNLIEYIKKENSHNPKGPLIFKMVVGELSEDTMEIVKNAASSKFDEISIIGGPQTFCEDKLEIYSLLDRFKSVKYHILPIRPNKHFMIFNQTHLYIEKPHHHNETRGAVGVKTAHPDLIQVYEDAYNEINKYAKQLNKEEVLKQACYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY2</accession>
    <name>F0TAY2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S17e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps17e</name>
        <name evidence="5" type="ordered locus">Metbo_1908</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645479.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1908"/>
    <dbReference type="EnsemblBacteria" id="ADZ10128">
        <property type="protein sequence ID" value="ADZ10128"/>
        <property type="gene ID" value="Metbo_1908"/>
    </dbReference>
    <dbReference type="GeneID" id="10278366"/>
    <dbReference type="KEGG" id="mel:Metbo_1908"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176720_0_1_2"/>
    <dbReference type="OMA" id="TRDFEHN"/>
    <dbReference type="OrthoDB" id="128227at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_S17e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_S17e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="RPS17e-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="PTHR10732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="SSF116820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="63" mass="7285" checksum="4D9E04AC4D5648B0" modified="2011-05-03" version="1">MGNIRTSFVKRTSKELIETYEGVFTTDFDENKKLVEEFTTVSTKHLRNKIAGYVTRLVRQGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB33</accession>
    <name>F0TB33_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1959</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
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        <property type="gene ID" value="Metbo_1959"/>
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    <dbReference type="GeneID" id="10278419"/>
    <dbReference type="KEGG" id="mel:Metbo_1959"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755692_0_0_2"/>
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    <dbReference type="OrthoDB" id="97267at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="HIT-like_sf"/>
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        <property type="entry name" value="ThiN"/>
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        <location>
            <begin position="223"/>
            <end position="332"/>
        </location>
    </feature>
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        <location>
            <begin position="317"/>
            <end position="321"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="366" mass="41176" checksum="6347CD13D8330715" modified="2011-05-03" version="1">MDHIEEIEKVKEGASILEKSSEFAIIIPEVHSNLVMALENAEKIEEVVGIPGRITNLNGMPKTVSSPDFLKTSHMARLALSMMKHDPSLRSAINIKYDPLILEICEKLGLKISSYDRTYEPEVVRKVEGGTIPWGVEEAIKKIDDIPDVIYHEGAWGKEPMICIVGTDAVELAEMAVCIARLYKVEKNSKVKVKPTIPSESNSVLFASSRKKWKDSKPIDGCIFCSIVDGNPEVKEKVLYNDGKNMVLMNLYPYNRGHLEVLPVKHYTDLNELSTEEIKDLFIIVQRTISLIRDVIKPDGINVGINLGEAAGASVDHLHIHIVPRFKYESGFMETTANTRVIEEDIDVMYSKFIEKLDILHFEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB39</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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            <authorList>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_1965"/>
    <dbReference type="EnsemblBacteria" id="ADZ10185">
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        <property type="gene ID" value="Metbo_1965"/>
    </dbReference>
    <dbReference type="GeneID" id="10278425"/>
    <dbReference type="KEGG" id="mel:Metbo_1965"/>
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    <dbReference type="OMA" id="WVRGCYF"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
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        <property type="entry name" value="CBS_dom_sf"/>
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        <property type="entry name" value="HcyBio"/>
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    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
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    <dbReference type="Pfam" id="PF00571">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
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        <location>
            <begin position="394"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="455"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="514" mass="55838" checksum="CDD655F895721602" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB41</accession>
    <name>F0TB41_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1967"/>
    <dbReference type="EnsemblBacteria" id="ADZ10187">
        <property type="protein sequence ID" value="ADZ10187"/>
        <property type="gene ID" value="Metbo_1967"/>
    </dbReference>
    <dbReference type="GeneID" id="10278427"/>
    <dbReference type="KEGG" id="mel:Metbo_1967"/>
    <dbReference type="HOGENOM" id="CLU_1801707_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15824" checksum="C7DA8167802E8498" modified="2011-05-03" version="1" precursor="true">MMKNIFWSFLIVGLMVAAGTGWASASNPAQLGLNSVKYPSQFENLQYNINGNNTISLNGTLTFTDNSTTKPIAGAYLFLAILGLTNDKVNHNIVYHGFCVTDKDGNFQFNSGPYFTGFPGNYTLLLRYHGNRTYDPCNLTFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB49</accession>
    <name>F0TB49_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645546.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10195">
        <property type="protein sequence ID" value="ADZ10195"/>
        <property type="gene ID" value="Metbo_1975"/>
    </dbReference>
    <dbReference type="GeneID" id="10278435"/>
    <dbReference type="KEGG" id="mel:Metbo_1975"/>
    <dbReference type="eggNOG" id="arCOG06453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118894_0_0_2"/>
    <dbReference type="OMA" id="SGFLMKI"/>
    <dbReference type="OrthoDB" id="122159at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="22322" checksum="1F6533AA2E934B04" modified="2011-05-03" version="1">MLLEPVLYAFSGFFMKFSDDAYDKKKNIYMAVMAAVLCGVSIAYLAVTSSDSACLFIAILIGTVLSLKVDSLNHVLALILFLVIIIYIGIPSIGIVTLILCTLAAFIDEVGNDNERIKKLGRGAELFFDYRFTLKLTVLVFAVLGLLVPYFPVLTQPNIQYFQFQTFIYFLLFDLFYEVAGLKFDAIYNGFNNLFRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB50</accession>
    <name>F0TB50_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="2">2.5.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645547.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1976"/>
    <dbReference type="EnsemblBacteria" id="ADZ10196">
        <property type="protein sequence ID" value="ADZ10196"/>
        <property type="gene ID" value="Metbo_1976"/>
    </dbReference>
    <dbReference type="GeneID" id="10278436"/>
    <dbReference type="KEGG" id="mel:Metbo_1976"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="OMA" id="FWSYFCQ"/>
    <dbReference type="OrthoDB" id="35308at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008612">
        <property type="term" value="P:peptidyl-lysine modification to peptidyl-hypusine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="PTHR11703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="304" mass="32665" checksum="B12C7181A2FFB6DC" modified="2011-05-03" version="1">MEIKKGMSVLELIQEMGGSGVLGAGRVYRATELLAEVIKDEDTAVFLSVAGPLVPGGLRKIIRNMIKDGEVDVLITSGANITHDLLEAFGGRHHRGIEEDDETMCEHGMGRIADLYTKSEDFEVFETEIAKILADIAAKDDHLSIREFLTLIGSSLDDDESILKTAADNNVPIYAPGFIDSMLGLQLWMFTQDTKLHIDASGDMAELSDIVYDSKKVATMILGGGLPKHYALASNLLKGGVDAAIQITMDRSETGSLGGAPLEEAKSWAKAKAGSKLVTVVGDATIIFPLILAGAKEMNQRSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB56</accession>
    <name>F0TB56_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645553.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1982"/>
    <dbReference type="EnsemblBacteria" id="ADZ10202">
        <property type="protein sequence ID" value="ADZ10202"/>
        <property type="gene ID" value="Metbo_1982"/>
    </dbReference>
    <dbReference type="GeneID" id="10278442"/>
    <dbReference type="KEGG" id="mel:Metbo_1982"/>
    <dbReference type="eggNOG" id="arCOG04347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070643_0_0_2"/>
    <dbReference type="OMA" id="ECEKMND"/>
    <dbReference type="OrthoDB" id="70610at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="47"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="263" mass="30488" checksum="340924BF8E9521EB" modified="2011-05-03" version="1">MGLITEQYKDSSNFVARIELNRKFSTNPQPLTRWLFDQMDFQKSSRVLELGCGNAILWRTNKDKIVSDEKIVLTDFSEGMLDDAKKVLGSLRDRFEFKVMDAENISFPENSFDIVIANLMLYHVPNRKEAFNQITKVLADNGLFYASTFGRNNMKELNELVQEFYPQLNSLETLSNRFGLENGKSQLEDYFEKVTMTEYHDHLEITDAQPVIEYFLSFAGNKSVLKPDKLDAMKTCINEVIEDNGFMKVTKETGLFRARYPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB59</accession>
    <name>F0TB59_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644361.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0759"/>
    <dbReference type="EnsemblBacteria" id="ADZ09010">
        <property type="protein sequence ID" value="ADZ09010"/>
        <property type="gene ID" value="Metbo_0759"/>
    </dbReference>
    <dbReference type="GeneID" id="10277207"/>
    <dbReference type="KEGG" id="mel:Metbo_0759"/>
    <dbReference type="eggNOG" id="arCOG02408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100862_1_1_2"/>
    <dbReference type="OMA" id="TIAYVTR"/>
    <dbReference type="OrthoDB" id="88507at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00288">
        <property type="entry name" value="TIGR00288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="163" mass="17903" checksum="F0AFD31E110DFA83" modified="2011-05-03" version="1">MRSFERLSSLKDYIPLKRSEAGGKNVGLLVDGPNMLRKEFSLNLDIVRDIIAEYGNMRVGKVLLNQYASDKLIEAIVNQGYTPIVVAGDTDVYMAVEAMELIYNPNIDIIALMTRDADFLPIINKAKENGKETLVIGAEPGFSAALQNSADSAIVLKSDNNHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB72</accession>
    <name>F0TB72_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0772"/>
    <dbReference type="EnsemblBacteria" id="ADZ09023">
        <property type="protein sequence ID" value="ADZ09023"/>
        <property type="gene ID" value="Metbo_0772"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0772"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1202632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="230" mass="26481" checksum="08AF146547754CE7" modified="2011-05-03" version="1">MVRSLEIENQNLNSQLKNLETENEDLVKENGILVEKLKILENLENRTLSTDCVRGVSSDAGKAAVLKYILEHVKKDATILDLGFGSGIYGKILRAFDYQTIDGVDVYNKNIQEMGLDRIYDGIFVSNIVDFDFEHYDLIIFGDVLEHIELEAAKDLLSGYIKDNKCEHILVSIPYEYEQEEVYGNQYEKHLQPMANREFMEEQYPYLKLVDEAIMPHNGGTIATYIWSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB86</accession>
    <name>F0TB86_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl24</name>
        <name evidence="4" type="ordered locus">Metbo_0786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="2">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644388.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0786"/>
    <dbReference type="EnsemblBacteria" id="ADZ09037">
        <property type="protein sequence ID" value="ADZ09037"/>
        <property type="gene ID" value="Metbo_0786"/>
    </dbReference>
    <dbReference type="GeneID" id="10277234"/>
    <dbReference type="KEGG" id="mel:Metbo_0786"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="OMA" id="VRIMRGD"/>
    <dbReference type="OrthoDB" id="107015at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_L24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_L26/L24P_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="PTHR11143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="3">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="117" mass="13894" checksum="3D78B25EC1DEA692" modified="2011-05-03" version="1">MSKQPRKQRKLIYKAPLHIRHKLMSVKLSEELREQYKRRSLPVKKGDTIKVMRGDFKDHEGKIEKVDLKNYRVMIEGMSVQKPDGNKVYHSVHPSNIMILELDLEDDERNEIIERKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH7</accession>
    <name>F0TBH7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10246">
        <property type="protein sequence ID" value="ADZ10246"/>
        <property type="gene ID" value="Metbo_2027"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2027"/>
    <dbReference type="HOGENOM" id="CLU_2662360_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="75" mass="8296" checksum="34AE43B57D6A3EF3" modified="2011-05-03" version="1">MVINASASNPQYAVMSFFAYPEGETKSYVGSGDMGTFSQSSQSDEFEVTASPGNYYLSVIAANLNKWHVEVFNYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ0</accession>
    <name>F0TBJ0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645610.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2040"/>
    <dbReference type="EnsemblBacteria" id="ADZ10259">
        <property type="protein sequence ID" value="ADZ10259"/>
        <property type="gene ID" value="Metbo_2040"/>
    </dbReference>
    <dbReference type="GeneID" id="10278501"/>
    <dbReference type="KEGG" id="mel:Metbo_2040"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_3_0_2"/>
    <dbReference type="OMA" id="TVADPHM"/>
    <dbReference type="OrthoDB" id="113911at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="96" mass="10209" checksum="E8ABBDC626B99115" modified="2011-05-03" version="1">MSEELVEKIRTALSTVADPHMGVSIVEMGLVTGIEVDEEAKTAKITITPTNPGCMSAANMAMQARTAAEGLDEIDKAEIVMEGHMMADAISEMVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ2</accession>
    <name>F0TBJ2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trpD</name>
        <name evidence="6" type="ordered locus">Metbo_2042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645612.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2042"/>
    <dbReference type="EnsemblBacteria" id="ADZ10261">
        <property type="protein sequence ID" value="ADZ10261"/>
        <property type="gene ID" value="Metbo_2042"/>
    </dbReference>
    <dbReference type="GeneID" id="10278504"/>
    <dbReference type="KEGG" id="mel:Metbo_2042"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="OrthoDB" id="77838at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1 3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycos_trans_3N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_3" evidence="4">
        <location>
            <begin position="75"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosylpyrophosphate binding" evidence="3">
        <location>
            <begin position="109"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="348" mass="37699" checksum="20EBA03EAFFC3F55" modified="2011-05-03" version="1">MISECLKKVVAGNDLTQDEAYTSMMEMTSGEAGEIRTAAFLTALSMKGETADEITGFVKAMRELCIEVSPETDLPLVDTCGTGGDRLKTFNISTTAAIIAAASDVAIAKHGNRSITSKCGGADILEALGVNINCDKSSVERCIETCKISFIFAPNFHPSMKRIMPVRQELGIRTVFNILGPLTSPANADIQLLGVFDPEYVEIIADVLKNLDVKHAMVVHGFDENDDPAMDEISTIGKTRVAILENGRVTVKDIYPEDFGVKRSKSRFIRAPETIEENIQVVKDVLSGKNSTTEERAKLDMCLVNAAAILYIAGQADSLKMGYQNAYATVKSGRALEKLGSFIEISNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ3</accession>
    <name>F0TBJ3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="8">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpA</name>
        <name evidence="10" type="ordered locus">Metbo_2043</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645613.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2043"/>
    <dbReference type="EnsemblBacteria" id="ADZ10262">
        <property type="protein sequence ID" value="ADZ10262"/>
        <property type="gene ID" value="Metbo_2043"/>
    </dbReference>
    <dbReference type="GeneID" id="10278505"/>
    <dbReference type="KEGG" id="mel:Metbo_2043"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="OrthoDB" id="65368at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="30039" checksum="3A279FD28C8808BF" modified="2011-05-03" version="1">MNPNNQKTKTYKEVFEELKNKNEGAFIPFVVAGDPNFETSIEIVKKFVENGADALEIGFPFSDPVADGPSVQTADIRALESGMTTEKCFKFIERIREFTQVPIGLLVYYNLIYRKGIETFYKRASEAGVTGILAADLPIEEAGSAVEMADKYGVNQIFMVAQTTRNERIDEIAKHASGFLYVVAVMGVTGARSDIQTSTVDLITRVKDRTDLPIAVGFGISKPEHVRDVIKSGSNGAIVASAIINIITANLDNKDKALEEIGKFCSELKQSTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">AL-21</strain>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0835"/>
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    <dbReference type="HOGENOM" id="CLU_342452_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="ADZ09085.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="745" mass="80166" checksum="6EAB271EA87CC61F" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ9</accession>
    <name>F0TBQ9_METLA</name>
    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lapidus A."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
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    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0887"/>
    <dbReference type="EnsemblBacteria" id="ADZ09136">
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        <property type="gene ID" value="Metbo_0887"/>
    </dbReference>
    <dbReference type="GeneID" id="10277336"/>
    <dbReference type="KEGG" id="mel:Metbo_0887"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_6_2_2"/>
    <dbReference type="OMA" id="HIANLFM"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
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        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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            <begin position="12"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="477" mass="53364" checksum="B90ADF6F5FE94799" modified="2011-05-03" version="1">MNTVQKISKNVGLVFVSQILTYVLAFFTVIYTARYLGAGGFGILSLALSIGSIFAVFMDMGLSTLIVREISRDKSLSGKYIINSIVMKLILSILTLGLVVVTVHLLGYNPLESLIIYLIAISMAISSFYIFLNAVFQAEEKMEYLSLANILNSIILLAGTILGVYLGLSIVYFALIYIFTSIIVLIYCVTVYVKKFLNIKLELDLGFWKPTLRESWSFGLISLSYMLYTYVDSIMLSILKGAEAVGWYSASYRLMYIALIIPNAINIAIFPVMSRLYTNASKNSLNLLNERYFKYMMVLGIPLGIGTSLLAKPIILLLYGTEYYPSIITLQILVWAIVFTFAGASYIQLLQSTNKQLIITKISLVCLVLNVILNLILIPSYSYVGASFATIATEAVLNGYIIYVTYKLGYGTSYKVVLKDLSKIFVATVTMTIFIWYFINLNFFVVVAVAIIIYFVTLYIVRGIDDVDIEILKQIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS9</accession>
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        <recommendedName>
            <fullName evidence="3">DUF3880 domain-containing protein</fullName>
        </recommendedName>
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    <gene>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644507.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0907"/>
    <dbReference type="EnsemblBacteria" id="ADZ09156">
        <property type="protein sequence ID" value="ADZ09156"/>
        <property type="gene ID" value="Metbo_0907"/>
    </dbReference>
    <dbReference type="GeneID" id="10277356"/>
    <dbReference type="KEGG" id="mel:Metbo_0907"/>
    <dbReference type="eggNOG" id="arCOG07799">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025555_0_0_2"/>
    <dbReference type="OrthoDB" id="64322at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024542">
        <property type="entry name" value="DUF3880"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12996">
        <property type="entry name" value="DUF3880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3880" evidence="3">
        <location>
            <begin position="244"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="626" mass="74102" checksum="D25F00EF4C07FDBC" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT3</accession>
    <name>F0TBT3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644511.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0911"/>
    <dbReference type="EnsemblBacteria" id="ADZ09160">
        <property type="protein sequence ID" value="ADZ09160"/>
        <property type="gene ID" value="Metbo_0911"/>
    </dbReference>
    <dbReference type="GeneID" id="24964524"/>
    <dbReference type="KEGG" id="mel:Metbo_0911"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_25_3_2"/>
    <dbReference type="OMA" id="IYFDLAM"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="487" mass="57965" checksum="92B2F72B5526DFAD" modified="2011-05-03" version="1">MEDVKVSIIIPVYNVQKYLRQCLDSVTNQTLKEIQIICVNDGSTDGSLDILEEYAQKDDRILVVNQENSGCAVARKTGLKHSTGEYIEFVDSDDWLELDALEKTYNNAISNHSDLVLFPISRYNDRENKYFNSGSTVENYFDDPTIDYNNFTFQIKDIKPFLLNSSFSVWFKLYKSDFIKGYDDFYFPRNMNFEDVPFHVQIMIRATKISFCPGKIYNYRTSNMDSITNSVYVKEKIFDIFEVINHVEKILIENDLREEYNFEFIRFKINQLGYWFQQSDERFKEELFLRIKKEFEALKLSDEDVDRLNHRLRNIYLHVSATNTLGEMVLFEEKLDLEEQLKQQKRQYTVQIKDMNQNFKNKFNEQRINLENTRANYEERILNQKKSHEKVTEHNLKRIENLQIAQSKNITAIKSLENDLNQIKTELKKILNTKPYRFAYFLHRFSHEFVKGDMTNKTDFIRWSLAQITGKKSDNEIKYNPLGKLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBV7</accession>
    <name>F0TBV7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645650.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2080"/>
    <dbReference type="EnsemblBacteria" id="ADZ10299">
        <property type="protein sequence ID" value="ADZ10299"/>
        <property type="gene ID" value="Metbo_2080"/>
    </dbReference>
    <dbReference type="GeneID" id="10278542"/>
    <dbReference type="KEGG" id="mel:Metbo_2080"/>
    <dbReference type="eggNOG" id="arCOG06475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134831_0_0_2"/>
    <dbReference type="OMA" id="DLPEHEY"/>
    <dbReference type="OrthoDB" id="114677at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17625" checksum="62BE9AE227FBD867" modified="2011-05-03" version="1">MLHITKKENRVLNQVKYFQNEYKEGVPYRILKLDLDMSEVDLKDILNNLDSKAIIKYSNSTAKLLETGNVNVWDSKEEVANEELNQTEEKAFQILKNFVGDDGKISKTLLEGQMLYGELKLSSLKTYNILASLENKGLISKIQLPDGDYYTLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW0</accession>
    <name>F0TBW0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="4">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645653.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2083"/>
    <dbReference type="EnsemblBacteria" id="ADZ10302">
        <property type="protein sequence ID" value="ADZ10302"/>
        <property type="gene ID" value="Metbo_2083"/>
    </dbReference>
    <dbReference type="GeneID" id="10278545"/>
    <dbReference type="KEGG" id="mel:Metbo_2083"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_2"/>
    <dbReference type="OMA" id="DGELGEW"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="325" mass="36619" checksum="A157AF29A7F30BA6" modified="2011-05-03" version="1">MILIVGGAGYIGSHINKMLSENGYETVVFDNLSYGHEDFVKWGHFERGDLGNIESIRKVFKKYTIDSVMHFAAFAYVGESVVDPQKYYRNNVLNTLNLLQVMLEFDVKRLVFSSTCATYGNPVEIPITENHPQNPINPYGRGKLMVETVLDDYSRAYDLNYVSLRYFNAAGADPEAEVGENHNPETHLIPLILDAAIGKRENIQIFGTDYETEDGTCIRDYIHVTDLADAHLKALKYLEDGGKSDYFNLGNGSGFSVQEVIERARQITGKTIVAVESDRRPGDPPVLVGSSDKIRSVLNWKPKYNDISVIIETAWNWHIKNNDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW8</accession>
    <name>F0TBW8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645661.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2091"/>
    <dbReference type="EnsemblBacteria" id="ADZ10310">
        <property type="protein sequence ID" value="ADZ10310"/>
        <property type="gene ID" value="Metbo_2091"/>
    </dbReference>
    <dbReference type="GeneID" id="10278554"/>
    <dbReference type="KEGG" id="mel:Metbo_2091"/>
    <dbReference type="eggNOG" id="arCOG00503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036012_0_0_2"/>
    <dbReference type="OMA" id="CSGWRAK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="278" mass="31215" checksum="48DB70FD3AE5F756" modified="2011-05-03" version="1">MRLSCVVENTATFSSEFWAEHGFSVLIEHEDSKILCDAGKSPEVLERNMGLINGFNDLETVVLSHGHSDHTGGLPAIFRNCSADIYMHKTGLKPKYLSKDGERKFIGIPEEYHSEYGNFSLKHSISSVKFVEKPVEIAPDIYIFTDIPMLNDFEQLSSSLLCLEDGNFVQDTFKEEVVVVVRTDNGLVIVSGCAHRGIINSINAVSDYFHENIYGVVGGTHLVAADVNRRLRTVQEFENIYATKLVLGHCNGFEAQCLFKNKFKEVFQPLECGKMMMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC01</accession>
    <name>F0TC01_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2124"/>
    <dbReference type="EnsemblBacteria" id="ADZ10343">
        <property type="protein sequence ID" value="ADZ10343"/>
        <property type="gene ID" value="Metbo_2124"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2124"/>
    <dbReference type="eggNOG" id="arCOG14014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3113114_0_0_2"/>
    <dbReference type="OMA" id="FFDILIW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="5891" checksum="182C2825F8FFC0E3" modified="2011-05-03" version="1">MNNKNTLMGICYIVVALLIVVVKYAVPQFFDILIWLIAIGLAATGVYFIMKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC26</accession>
    <name>F0TC26_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644528.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0930"/>
    <dbReference type="EnsemblBacteria" id="ADZ09177">
        <property type="protein sequence ID" value="ADZ09177"/>
        <property type="gene ID" value="Metbo_0930"/>
    </dbReference>
    <dbReference type="GeneID" id="10277379"/>
    <dbReference type="KEGG" id="mel:Metbo_0930"/>
    <dbReference type="HOGENOM" id="CLU_2127843_0_0_2"/>
    <dbReference type="OMA" id="HNKKEAY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="113" mass="11304" checksum="CAB913E16DA1E083" modified="2011-05-03" version="1" precursor="true">MKQKLTIIPVLVILVVLGGVLISGCIGNNNTNNTTVNNTTYSSPANATTTANTTTTTASSSKSTSSDNSKQNTSSQSSGSKNSGSTSGTSGSNDGLTGKNYNANPTDPNGNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC41</accession>
    <name>F0TC41_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0945</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644543.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0945"/>
    <dbReference type="EnsemblBacteria" id="ADZ09192">
        <property type="protein sequence ID" value="ADZ09192"/>
        <property type="gene ID" value="Metbo_0945"/>
    </dbReference>
    <dbReference type="GeneID" id="10277394"/>
    <dbReference type="KEGG" id="mel:Metbo_0945"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="OMA" id="LILEMAV"/>
    <dbReference type="OrthoDB" id="104092at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="50"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="55"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="18071" checksum="00C5EB40E3BA3D47" modified="2011-05-03" version="1">MTDKDVEIKTEEEINNEEDESKRIPSESVLSDIITSIKEKQEDLGKSLSDYSKSLQKPLADIIETDDALKVVTDLPGVKKEDVEVNISEDSLEIVAKFDDEITEEGTNYLKRERSYGETTRTIALPEKIDTKKASAKFNDSVLTVELPKIKEVKHKVNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC56</accession>
    <name>F0TC56_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="6">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="6">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="6">Ornithine acetyltransferase</fullName>
                <shortName evidence="6">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="6">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="6">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="6">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="6">N-acetylglutamate synthase</fullName>
                <shortName evidence="6">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="6">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">argJ</name>
        <name evidence="7" type="ordered locus">Metbo_0960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="6"/>
    <dbReference type="EC" id="2.3.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644558.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0960"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="EnsemblBacteria" id="ADZ09207">
        <property type="protein sequence ID" value="ADZ09207"/>
        <property type="gene ID" value="Metbo_0960"/>
    </dbReference>
    <dbReference type="GeneID" id="10277409"/>
    <dbReference type="KEGG" id="mel:Metbo_0960"/>
    <dbReference type="eggNOG" id="arCOG04413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_2"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="OrthoDB" id="29910at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023472009" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="6">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023472011" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="6">
        <location>
            <begin position="184"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="6">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="6">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="6">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="398" mass="41555" checksum="FBC1540BABA47241" modified="2011-05-03" version="1">MKIVDGGVCAVNGVLAAGACDDDYGVAVIVCKDSDSTAVFTSNKIVAAPVILTKNAVENGKLSAVVANSGNANCFTGDQGIEDGKAMASVVAEELDFKFEDVAVASTGVIGRLMPMDIIGSLIKKAVLRLENSAEASKNAAEAIMTTDTYSKEFAVETTLKNGKSIRIGGITKGSGMIAPNMGTMLCFITTDVKASSSELNTALKKAVDASFNMVVVDGDESTNDTVILMANGESGESIDENFEEALEYVCCQLASMMAKDGEGATKFMEVEVNGAVNTEEARLAAKAVVSSSLVKTALFGADPNWGRIVAAVGYSGADIDPKVVSVSLQSLNKRVDIVDHGIIAAFEGTSQLEEAEDIMEEKNIRIIIDLGLGNGNATAYGCDLSYDYVSINAEYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC88</accession>
    <name>F0TC88_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645706.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2136"/>
    <dbReference type="EnsemblBacteria" id="ADZ10355">
        <property type="protein sequence ID" value="ADZ10355"/>
        <property type="gene ID" value="Metbo_2136"/>
    </dbReference>
    <dbReference type="GeneID" id="10278599"/>
    <dbReference type="KEGG" id="mel:Metbo_2136"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_5_0_2"/>
    <dbReference type="OMA" id="KNIWNAN"/>
    <dbReference type="OrthoDB" id="108307at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11838" checksum="F53DE9A0ABC749CD" modified="2011-05-03" version="1">MKDEVFFSKGMNPIKEDYPDLYDISVSLNDVCYTGKSLDYKTQKLVAIGIAAASSDNRAIKKQILSGMKELDITKEEVMDVLRVVLLLSGKPAFMKGINALYNIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCB9</accession>
    <name>F0TCB9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645737.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2171"/>
    <dbReference type="EnsemblBacteria" id="ADZ10386">
        <property type="protein sequence ID" value="ADZ10386"/>
        <property type="gene ID" value="Metbo_2171"/>
    </dbReference>
    <dbReference type="GeneID" id="10278634"/>
    <dbReference type="KEGG" id="mel:Metbo_2171"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173378_0_0_2"/>
    <dbReference type="OMA" id="KQIGPHV"/>
    <dbReference type="OrthoDB" id="77231at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol_B" evidence="2">
        <location>
            <begin position="11"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="25026" checksum="E53B95964DA2E1E7" modified="2011-05-03" version="1">MVCLLNEQILNKVEEFLGDVNKNLPEGMELEYEGFYERGFFVTKKRYALIENKNIVVKGLELVRRDWAPVAKKTQEQVLRAILEEANPKKAAKIIKSVVEEIKEGKIPLEDLVIHTQITKNPEDYVQMAPHVLAAKRAIANGRKISRGSIIRYVIVRGKDPISKRAIPLEDVDVSDYDPTYYIENQVLPAVSRIVEALGYSKEDILHKEKQVSLDSYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD1</accession>
    <name>F0TCD1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L37Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl37ae</name>
        <name evidence="3" type="ordered locus">Metbo_2183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645749.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2183"/>
    <dbReference type="EnsemblBacteria" id="ADZ10398">
        <property type="protein sequence ID" value="ADZ10398"/>
        <property type="gene ID" value="Metbo_2183"/>
    </dbReference>
    <dbReference type="GeneID" id="10278646"/>
    <dbReference type="KEGG" id="mel:Metbo_2183"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="OMA" id="ISTGIWQ"/>
    <dbReference type="OrthoDB" id="122290at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_L37Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_L37ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="89" mass="9736" checksum="23E7F27584D098B0" modified="2011-05-03" version="1">MARTKKVGITGRFGARYGRKAKRTVKSVEENMKVKHVCPQCDRPAVKRVSAGIWKCRKCGNVFTGGAYLPSTPMGKTAARNIKRIVGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE3</accession>
    <name>F0TCE3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative thymidylate synthase</fullName>
            <shortName evidence="2">TS</shortName>
            <shortName evidence="2">TSase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thyA</name>
        <name evidence="4" type="ordered locus">Metbo_2196</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May catalyze the biosynthesis of dTMP using an unknown cosubstrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645761.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2196"/>
    <dbReference type="EnsemblBacteria" id="ADZ10410">
        <property type="protein sequence ID" value="ADZ10410"/>
        <property type="gene ID" value="Metbo_2196"/>
    </dbReference>
    <dbReference type="GeneID" id="10278659"/>
    <dbReference type="KEGG" id="mel:Metbo_2196"/>
    <dbReference type="eggNOG" id="arCOG03214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084975_0_0_2"/>
    <dbReference type="OMA" id="HHSVSMH"/>
    <dbReference type="OrthoDB" id="57461at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01686">
        <property type="entry name" value="Thymidy_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03283">
        <property type="entry name" value="thy_syn_methano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="3">
        <location>
            <begin position="34"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="205" mass="23093" checksum="E62AD2791C79CFC6" modified="2011-05-03" version="1">MAVMIKVPTIKKGWETLVKKIVSKGVEIKDERGSVTKELLNTVVNVTDPSNTESPEGYFWTGEKLDKYSEQFLSGDKQGFVYTYGNRLRAHFGGIDQIQEAVMRLNNFKESRRAISITWDPLVDTQNDEVPCMMLVDFKIRDGELHTTGLWRSHDIYGAWFPNAVGLSNLAKYVAEETGSKLGTLTIHSISAHIYEVNFKEAGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF0</accession>
    <name>F0TCF0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="2">RPPK</shortName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="2">P-Rib-PP synthase</shortName>
            <shortName evidence="2">PRPP synthase</shortName>
            <shortName evidence="2">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prs</name>
        <name evidence="5" type="ordered locus">Metbo_2203</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645768.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2203"/>
    <dbReference type="EnsemblBacteria" id="ADZ10417">
        <property type="protein sequence ID" value="ADZ10417"/>
        <property type="gene ID" value="Metbo_2203"/>
    </dbReference>
    <dbReference type="GeneID" id="10278667"/>
    <dbReference type="KEGG" id="mel:Metbo_2203"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="OrthoDB" id="59314at2157"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="165"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="2">
        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="91"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="31571" checksum="201268A5A82AC3E2" modified="2011-05-03" version="1">MLIGGSASQKLAAKVARELDQKLNPVETRRFPDGERYIRIKGEVLNEAVVIQSTGHPQDANLMELFLMLKNLKSLGVERIRVVIPYFGYGRQERRFKSGEAVSAVIVSELLEAAGASEIYSINFHEKNIKEFFSIPVHEISAMPLIANYIAKTMNNPLILGPDKGALGFAEEIAEILDCECDHLEKVRISPEHVETKPKHIDVDGREVVIIDDIISTGGTIVNATKILKDLGAKKVVVGCVHPVLVEDALLKIFAAGVDDVFSTDTLRSDVSNISVAPLVAACLKKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH5</accession>
    <name>F0TCH5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1005</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="7 8">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644603.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1005"/>
    <dbReference type="EnsemblBacteria" id="ADZ09252">
        <property type="protein sequence ID" value="ADZ09252"/>
        <property type="gene ID" value="Metbo_1005"/>
    </dbReference>
    <dbReference type="GeneID" id="10277454"/>
    <dbReference type="KEGG" id="mel:Metbo_1005"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_3_3_2"/>
    <dbReference type="OMA" id="CLECHYI"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="55" mass="6365" checksum="B4D500D57696F7DA" modified="2011-05-03" version="1">MERYRCGICGYVYDPEKGESRNNTAPGTDFEDLPDMWFCPSCGAAKRRFKITKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ0</accession>
    <name>F0TCJ0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644618.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1020"/>
    <dbReference type="EnsemblBacteria" id="ADZ09267">
        <property type="protein sequence ID" value="ADZ09267"/>
        <property type="gene ID" value="Metbo_1020"/>
    </dbReference>
    <dbReference type="GeneID" id="10277469"/>
    <dbReference type="KEGG" id="mel:Metbo_1020"/>
    <dbReference type="eggNOG" id="arCOG01680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136066_0_0_2"/>
    <dbReference type="OrthoDB" id="49247at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="193"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="28757" checksum="F7459D71AE525F7B" modified="2011-05-03" version="1">MTGTKKSSNNKNSQSYKIFSSKKHVDVVKGQVKVKILDALCKKNMSFNEIVELTGKAKPTVSQHLSELVTNGLIVSIKDPNDKRRKIFSINAEFLGELSSDNEAKFDIEEYFSNISNSSDPFDFYRLILRAVRTEFWNDGINIDPILHKAGIRVGKVFYNELQGEDLESFLGNIEKFWEEKHLGRVEVKTLDPIVICVYDCFECKDLPEIGEPACSFDSGLLDAIFSKYLDKDVTTIETTCFAMGDECCTFVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM6</accession>
    <name>F0TCM6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1056"/>
    <dbReference type="EnsemblBacteria" id="ADZ09303">
        <property type="protein sequence ID" value="ADZ09303"/>
        <property type="gene ID" value="Metbo_1056"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1056"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_19_4_2"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="17"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="398" mass="42947" checksum="9B6C86945969A439" modified="2011-05-03" version="1" precursor="true">MNKSITTIKSKSNYSLMTLILFWCGLIVMSSMYLTIPLISLFSSTFDVPSGVAAWTSSIFCIFFAVGCLVYGPMSDRYGRKKIILIGMTMLTVITPLVGLFDNIYWILTMRALQGAVAASFSPVALAYIPEMFPDDKKLTATGFLVTGFLMAGIVGQVISGLINQYLAWNYVFYIMGLTYLVTLVLVLLLLPNDNLPRLKTSIIQTVENMASLLKIKPLILCFAVDVMFLMSMMCMYTSLGYYLIEPQFGLNSQEILYVRAAGIFGIIFAATSGLFVKKFGLFNVLIGGLLIAAISLTSIAFVSSIQILVLLSVIFVAGIAVTAPALITIIGQIGGQSRGAALSLHTVILFIGAGIGPILAITLLDTGINILPYLVMGLILLLGVGLSLLIKKSVKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS7</accession>
    <name>F0TCS7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl12</name>
        <name evidence="6" type="ordered locus">Metbo_2253</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645818.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2253"/>
    <dbReference type="EnsemblBacteria" id="ADZ10467">
        <property type="protein sequence ID" value="ADZ10467"/>
        <property type="gene ID" value="Metbo_2253"/>
    </dbReference>
    <dbReference type="GeneID" id="10278719"/>
    <dbReference type="KEGG" id="mel:Metbo_2253"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="OMA" id="EYIYAAM"/>
    <dbReference type="OrthoDB" id="128352at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_L12/P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_L12_arc"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="100" mass="10522" checksum="DC05D0B233BBDAEF" modified="2011-05-03" version="1">MEYIYTAMLLHTTGQEISEENVKKVLEAAGAEVDEARVKALIAALEDVDIEEAMEKTAVAAAAPVAAAAAPAAAEEEEEEEEEEEEKEEEAAAGLGALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU2</accession>
    <name>F0TCU2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645833.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2268"/>
    <dbReference type="EnsemblBacteria" id="ADZ10482">
        <property type="protein sequence ID" value="ADZ10482"/>
        <property type="gene ID" value="Metbo_2268"/>
    </dbReference>
    <dbReference type="GeneID" id="10278734"/>
    <dbReference type="KEGG" id="mel:Metbo_2268"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="FYYKLGK"/>
    <dbReference type="OrthoDB" id="60124at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="226" mass="24192" checksum="00700BFC2756AB90" modified="2011-05-03" version="1">MSSCDVNGYEELSNKMKTKLGLEKSPVAVKLVLREEDIPEGISKIDSKLRHCEMVQKAAQGETFYATVEEQLCKGGAGAIGLMEPPEKVKTGEMYYSLERFSSLGAAKRTVDAIPKIDPIMKAIIYAPLETAPFDPDVVVIIGNSKQAMMLAQAMVFTLGGRIEADFAGIQSICGDAVAGPYTTKKPNFTLGCSGSRKYAGIKTDDVIVGMNGENIGCVVNALEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU6</accession>
    <name>F0TCU6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polymorphic membrane protein Chlamydia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645837.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2272"/>
    <dbReference type="EnsemblBacteria" id="ADZ10486">
        <property type="protein sequence ID" value="ADZ10486"/>
        <property type="gene ID" value="Metbo_2272"/>
    </dbReference>
    <dbReference type="GeneID" id="10278738"/>
    <dbReference type="KEGG" id="mel:Metbo_2272"/>
    <dbReference type="eggNOG" id="arCOG02552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="392"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="494"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="593"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="2">
        <location>
            <begin position="692"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="790" mass="84971" checksum="EEE8DD98305863D6" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T5</accession>
    <name>F0T5T5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="10">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_1083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALAD family.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
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        <property type="entry name" value="ALAD"/>
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    </dbReference>
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        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="131"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ09328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="323" mass="36290" checksum="C79BA4DA0DD6A64D" modified="2011-05-03" version="1">MEFPVTRMRRLRKDQNIRNILTETKLNPEDFIYPIFINEALEDGNKEEIKTMPGQFRFSINDAVTYLKELESIGLSSVILFGMPEIKDEKGSMAYHKNGVIQQTIRKLKAETDLVVITDVCLCEYTSHGHCGIIKDGEVLNDPTLNYLSKVALSHAEAGADIVAPSDMMDGRVASIRKVLDLNGYQDTIIMSYSAKYASAFYAPFREAVCSTPSFGDRKTYQMNPANSQEALREVKLDIAEGADMIIVKPAMPYMDIIREVKDEFKMPTVAYQVSGEYSMIMAGIENGYLTQDSIYESLLSIKRSGADLIISYFAPEFLEGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5T9</accession>
    <name>F0T5T9_METLA</name>
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        <submittedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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    </dbReference>
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        <property type="gene ID" value="Metbo_1087"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_1087"/>
    <dbReference type="eggNOG" id="arCOG02053">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_1_2"/>
    <dbReference type="OMA" id="YYEKMAR"/>
    <dbReference type="OrthoDB" id="99962at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00582">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="148" mass="16293" checksum="8AFE40D85A12CDF7" modified="2011-05-03" version="1">MHKKILLPTDGSENAIKAGEHAISLADMSGAEIIILNVIDTSYLDAIPQPEVREDVDEELRNDMKNAVKKFEETIEENQCSGTCKNINFKVLLKEGNPADVILKTIDDEGIDQVIMGKSGKQGLEKFLLGRTTDKIVKESKVPVNVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U1</accession>
    <name>F0T5U1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09334">
        <property type="protein sequence ID" value="ADZ09334"/>
        <property type="gene ID" value="Metbo_1089"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1089"/>
    <dbReference type="eggNOG" id="arCOG10842">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3094148_0_0_2"/>
    <dbReference type="OMA" id="WIVVAKC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="51" mass="5622" checksum="C1C84546A81C05C6" modified="2011-05-03" version="1">MDWIVVAKCDTCDQEKTYEVSGNTVPYSIGDFIENCSCGGQFIVDEILEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U8</accession>
    <name>F0T5U8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644692.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1096"/>
    <dbReference type="EnsemblBacteria" id="ADZ09341">
        <property type="protein sequence ID" value="ADZ09341"/>
        <property type="gene ID" value="Metbo_1096"/>
    </dbReference>
    <dbReference type="GeneID" id="10277546"/>
    <dbReference type="KEGG" id="mel:Metbo_1096"/>
    <dbReference type="eggNOG" id="arCOG00499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074581_0_0_2"/>
    <dbReference type="OMA" id="HCHPDHY"/>
    <dbReference type="OrthoDB" id="107971at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="26"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="258" mass="28830" checksum="52B3E12A395647B4" modified="2011-05-03" version="1">MAVVFATITQKRMTGGFRLEDFEGKNIHVDPGPGALVRTYQFGLSPMKINILMVSHCHTDHYTDAEVLLEAMTRGMTRKKGTIVGSTSVINGYERWGPCISKYHLSKPEVVTLEHGEEHRSDKLRITATRTVHGDPKAVGFKISGPDLTVSYTSDTEYTPDLHLDHKGVDVLIASIIRSGSERIRGHMSVDDFITLINEVKPKLAIMTHLGMKFIMESPEREAQRVTKETGVKTLAARDGMKIELDDLVTKQHTLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5V7</accession>
    <name>F0T5V7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644701.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09350">
        <property type="protein sequence ID" value="ADZ09350"/>
        <property type="gene ID" value="Metbo_1105"/>
    </dbReference>
    <dbReference type="GeneID" id="24964530"/>
    <dbReference type="KEGG" id="mel:Metbo_1105"/>
    <dbReference type="eggNOG" id="arCOG06693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014153_0_0_2"/>
    <dbReference type="OrthoDB" id="89386at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="274" mass="32458" checksum="156275E7FD3C0F0E" modified="2011-05-03" version="1">MIKTEFKNQILKLKNDITKIEAIMEDVSTNGSHNIQDCDEKYKKIHFSINKKIHNFEKQGLKLLHLNSHQSLKDFYSYWKINLPTYQSRREYIRSLYNPMEQKINKILIEIDDDDYGTIDLKELEIDNDKGKFELKKDELEIDEKLCFVLMPFDEKFNSIYNTIKEIVEDDEFALNCKRADEIFTIGVIIEDIWNYIQKARILVAELTEKNPNVFYELGLAHAMNKEVILITQNVDDIPFDLGHHRYIVYENSIDGAEKLKEALKNTLREQLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W6</accession>
    <name>F0T5W6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Restriction modification system DNA specificity domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644710.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1114"/>
    <dbReference type="REBASE" id="33846">
        <property type="entry name" value="S.Msp21ORF1113P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09359">
        <property type="protein sequence ID" value="ADZ09359"/>
        <property type="gene ID" value="Metbo_1114"/>
    </dbReference>
    <dbReference type="GeneID" id="25394724"/>
    <dbReference type="KEGG" id="mel:Metbo_1114"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_10_1_2"/>
    <dbReference type="OMA" id="NPKGWPV"/>
    <dbReference type="OrthoDB" id="102840at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="8"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="403" mass="47149" checksum="57B113D3AB56C3CE" modified="2011-05-03" version="1">MFTEGDLPKVWKIKKLTEICDVRDGTHDSPKYKNEGYPLVTSKNVATGFIDFSDVNLISKDDYDNINKRSYVDDGDILMPMIGTIGNPIIVKKDRKFAIKNVALIKFTKTDVSNKYVKLFLESIHFKHYIKKINRGGTQKFISLKDIRNFPVILPPIEKQNKLIKILEKAEKIKEWRVEADKLTDEYLKSVFLEIYNSASHHPDLKADYLSESLRDVKNGLSRRRKISENKGDIVLRLKDIRENKIDLTELNRIPLNELEKEKYGVERNDLLFIRVNGNKDYVGRCAVFREFNENIYFNDHIMRVKIDSNQFNPIFLAFLINSEYGKKQLKNHLRTSAGQYTINQKGLERLKFYQPDISLQNSFVDLFNKIEILKKDQFNSEIKIKELFDTLMQKAFKGELVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X9</accession>
    <name>F0T5X9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1128"/>
    <dbReference type="EnsemblBacteria" id="ADZ09372">
        <property type="protein sequence ID" value="ADZ09372"/>
        <property type="gene ID" value="Metbo_1128"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1128"/>
    <dbReference type="HOGENOM" id="CLU_2056068_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="12636" checksum="3B1948362768BCFB" modified="2011-05-03" version="1" precursor="true">MNIKLKTLAIGIIINILLGLILSYLLGGIGLTVALILAAVYVGFKVEKNYKVGALNGAILGIIFGICFGILLDNSLGLLYLRAIGLVIFLLLIPTVIFGIIGAISSIIGIYINEKHQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Z8</accession>
    <name>F0T5Z8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ordered locus">Metbo_2291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2291"/>
    <dbReference type="EnsemblBacteria" id="ADZ10505">
        <property type="protein sequence ID" value="ADZ10505"/>
        <property type="gene ID" value="Metbo_2291"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2291"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OMA" id="IAFRCNF"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="403" mass="43346" checksum="47BF739D523F19BF" modified="2011-05-03" version="1">MIIDGMGDRPIKELGDKTPLEAAKTPNMDKMVERGICGIMDPIRPGIRAGSDTSHISILGYDPYEVYTGRGPFEAAGVGLKVLPGDIAFRCNFSTADSDGIITDRRAGRIREGTEEIAAAINGMVIADDVEVIFKESTGHRAVLVLRGEGLSDQISDADPKHEGKPPKKVVPLDDTKEAARTADILNMIVSKSYDVLKDLPINLERIEAGENPANIIIPRGAGAVPYVEPFGDKYGLKPVCIAETGLIKGIGNITGMDLIDIEGATGGIDTNLENIERGILDTAALDYDFLLINVDGADEAGHDGQLDEKIKFIEKVDDMLARLMEIEDAYFILTADHSTPISTMDHTGDPVPILINGPEVKVDDVKIFSERAAAKGGLCRIQGCNVMDILMDLMNKSTKFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T600</accession>
    <name>F0T600_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="7">2.3.1.157</ecNumber>
            <ecNumber evidence="8">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_2293</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="7"/>
    <dbReference type="EC" id="2.7.7.23" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645858.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2293"/>
    <dbReference type="EnsemblBacteria" id="ADZ10507">
        <property type="protein sequence ID" value="ADZ10507"/>
        <property type="gene ID" value="Metbo_2293"/>
    </dbReference>
    <dbReference type="GeneID" id="10278759"/>
    <dbReference type="KEGG" id="mel:Metbo_2293"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="61185at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="10">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="426" mass="46850" checksum="E2256F5BCDF7E481" modified="2011-05-03" version="1">MNAIILTAGEGTRMRPLTITKPKTMLQIGGKPILQYNIESLRDAGVDEITLVVGYHEEVIKDHFKDGKDIGVKISYVTQEDRLGTAHAIGSARKHVHGQFITLNGDIIVDPALITELIDGYREENARSMLVLTEVEDPSSFGVVELDGNRIIRIVEKPKKEEAPSNLINAGIYLFDDQIFDAIDQTPKSERGEYEITDSLQLQMNEDENVVGLRSTNRWIDIGRPWELLDVNEHFLKDLETDIQGEVEEGVTIHGQVFIGKNSVVRSGCYIMGPVYIGENCDIGPNSFMRKYTSVGNNVSVGNAVELKNSIIMDNTNVNHLSYVGDSIIGSNCNIAAGTNIANLRFDDGNVMIVVKNEKIDSGRRKMGVVFGDGVKTGINSSFNPGVKVGVNSRIGAGVILSKDLESNKLLIAVQEHQIIENNKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T609</accession>
    <name>F0T609_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645867.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2302"/>
    <dbReference type="EnsemblBacteria" id="ADZ10516">
        <property type="protein sequence ID" value="ADZ10516"/>
        <property type="gene ID" value="Metbo_2302"/>
    </dbReference>
    <dbReference type="GeneID" id="10278768"/>
    <dbReference type="KEGG" id="mel:Metbo_2302"/>
    <dbReference type="eggNOG" id="arCOG08236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106303_2_0_2"/>
    <dbReference type="OMA" id="LHVTYFS"/>
    <dbReference type="OrthoDB" id="121923at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012545">
        <property type="entry name" value="DUF1697"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36439">
        <property type="entry name" value="PTHR36439"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08002">
        <property type="entry name" value="DUF1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008502">
        <property type="entry name" value="UCP008502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="181" mass="20860" checksum="02F5242D3E104E01" modified="2011-05-03" version="1">MTTYISMVRGINVGSKRIKMADLRDIYTFLGFKPVKTYIQSGNVIFKSNIDDPDELGQRIAQKIFETYNYHVEIVIRTMEELKTVVQSCPFGNKEVEYLHVTFLSAIPGGDRVRSIDMENINGIKSTEEFKLLSKEIYLYLPEGYGRTKLNNNFFEKKLGLNATTRNWKTVNKLLEIAESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T629</accession>
    <name>F0T629_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein P-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in the regulation of nitrogen fixation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645887.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2322"/>
    <dbReference type="EnsemblBacteria" id="ADZ10536">
        <property type="protein sequence ID" value="ADZ10536"/>
        <property type="gene ID" value="Metbo_2322"/>
    </dbReference>
    <dbReference type="GeneID" id="10278788"/>
    <dbReference type="KEGG" id="mel:Metbo_2322"/>
    <dbReference type="eggNOG" id="arCOG00362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_917070_0_0_2"/>
    <dbReference type="OMA" id="EPSRIND"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="305" mass="34461" checksum="F4F934DD6D9CE1E5" modified="2011-05-03" version="1">MGYKTNILIIGPSNSGKDTVVNNVEWGETRIMVASYASVVLNNNKNYLFMLKGEDNYKSFDEILLFQDGQGPDGIIMVLDSSKGFKENDLDVVEMIISQEIPHVIFANKQDMGNQNLNNHFTNSLIVPTIANEGIGINDGFKLLLKSVNDDSELTNSKPLPKVKTEFKREEPSRINDNDMVAKMKDALIPSKNHGICRLKMSMHPVELENVMKVLENNGFANMTVIKNRLVQDVGSKEIYRGYKHEARMKMRTELIMTIRSDEVSYVVDAIKSIKTDDIDDYISIKPVEDVLRVRTLERGVNALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T643</accession>
    <name>F0T643_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645901.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2337"/>
    <dbReference type="EnsemblBacteria" id="ADZ10550">
        <property type="protein sequence ID" value="ADZ10550"/>
        <property type="gene ID" value="Metbo_2337"/>
    </dbReference>
    <dbReference type="GeneID" id="10278803"/>
    <dbReference type="KEGG" id="mel:Metbo_2337"/>
    <dbReference type="eggNOG" id="arCOG04390">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202284_0_0_2"/>
    <dbReference type="OMA" id="VEPHKHC"/>
    <dbReference type="OrthoDB" id="131035at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019216">
        <property type="entry name" value="DUF2116_Zn-ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09889">
        <property type="entry name" value="DUF2116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004990">
        <property type="entry name" value="UCP004990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="66" mass="7788" checksum="BDAA719BF83A28BB" modified="2011-05-03" version="1">MTDQHKHCPVCQKPIPLSERFCSPKCEQIYMERQNKVAKTKKTLYVLFAAFIIIWLLFTLRGQLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T662</accession>
    <name>F0T662_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10569">
        <property type="protein sequence ID" value="ADZ10569"/>
        <property type="gene ID" value="Metbo_2356"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2356"/>
    <dbReference type="eggNOG" id="arCOG00567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504010_0_0_2"/>
    <dbReference type="OMA" id="VEERYIW"/>
    <dbReference type="OrthoDB" id="87795at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="519" mass="59790" checksum="FEE83F7704FD828E" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T686</accession>
    <name>F0T686_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
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        <property type="protein sequence ID" value="ADZ09401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ADZ09401">
        <property type="protein sequence ID" value="ADZ09401"/>
        <property type="gene ID" value="Metbo_1157"/>
    </dbReference>
    <dbReference type="GeneID" id="10277607"/>
    <dbReference type="KEGG" id="mel:Metbo_1157"/>
    <dbReference type="eggNOG" id="arCOG07604">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OMA" id="MESEIIN"/>
    <dbReference type="OrthoDB" id="56843at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="268" mass="29337" checksum="89476275D9E7BE5B" modified="2011-05-03" version="1">MDPVTVILMLGLFILMLVFIFSTALLTPLIGKKNILFVVLVGFTVGAVGGAFFISPIIDDVPDMANSLFHATSSSTDVINLNVSTDVNITSFLQNTKKIDGVKSVQSNGITITTPEMSQDWQTTYLNRIKTVNENITSIQIPNNKTILLEVKNDTNPMDVVNSLETWMMYVSGSTISYSVANVTLVVEASKLNQVMSQLPQGEIIITSINGTTENSVQAVKNVIPNKNDIILFCGFLGVLVGLAGLFIDSISRVLDQLKERIKEYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T689</accession>
    <name>F0T689_METLA</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644755.1">
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1160"/>
    <dbReference type="EnsemblBacteria" id="ADZ09404">
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        <property type="gene ID" value="Metbo_1160"/>
    </dbReference>
    <dbReference type="GeneID" id="10277610"/>
    <dbReference type="KEGG" id="mel:Metbo_1160"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="72601at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="227" mass="25350" checksum="E184EBF495B0B488" modified="2011-05-03" version="1">MDCVEMRNELKELLKLDNEIVAISWSVNEPKNVEKETEKSRFCSKLEKAMNGQMFYSTLEEEECMGGARYSGLKNMADYPANVQSGAFMVPKGLYKNIPAVQRSRETESYINPGIFKAIIFAPLKHVTWEPDVIFMVCNAKQGMEILHANAYDSGEHGKGADAAPICSSMAANPYMSGKVTYGFGDVASRNNMNIKPEEIMISIPGSDISRIVSNLVEMKTKMFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T696</accession>
    <name>F0T696_METLA</name>
    <protein>
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        </submittedName>
    </protein>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1167"/>
    <dbReference type="EnsemblBacteria" id="ADZ09411">
        <property type="protein sequence ID" value="ADZ09411"/>
        <property type="gene ID" value="Metbo_1167"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1167"/>
    <dbReference type="eggNOG" id="arCOG07792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121278_0_0_2"/>
    <dbReference type="OMA" id="TISMNIP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="144" mass="14884" checksum="9098DEB9B1DA6F2F" modified="2011-05-03" version="1">MDETGQASVEYVLLSLVFLIILGSVTIPMISKSIDSSMDVSDTSNVNSAVNSIANAVGVVYANGPGAKRTINVYFPVAGTLSWSNNAIGMPVTLTNGTAKNINAAVPFTVVFKPSGNVAQKTNYNATVEWAVGGASINVTLTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A3</accession>
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        <submittedName>
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    </protein>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09418">
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        <property type="gene ID" value="Metbo_1174"/>
    </dbReference>
    <dbReference type="GeneID" id="10277624"/>
    <dbReference type="KEGG" id="mel:Metbo_1174"/>
    <dbReference type="eggNOG" id="arCOG04396">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OMA" id="ERGTIPV"/>
    <dbReference type="OrthoDB" id="104907at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18033" checksum="0E81B6B12491A766" modified="2011-05-03" version="1">MRLNLVLELLDVPGQLIDALEPIGKLGANIVTVIHQRDVKTERGTIPVHLTLEGDEETLKRVLKTIESNDIQIIEVDGVVKKETITTILIGNIVDHDLKETVRELNSIDGVRVADLNLKMSDNPENSASRIVIETDYGRKAELMQNIKDFGKSKGFLVINEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6D2</accession>
    <name>F0T6D2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1204</name>
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    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644798.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1204"/>
    <dbReference type="EnsemblBacteria" id="ADZ09447">
        <property type="protein sequence ID" value="ADZ09447"/>
        <property type="gene ID" value="Metbo_1204"/>
    </dbReference>
    <dbReference type="GeneID" id="10277654"/>
    <dbReference type="KEGG" id="mel:Metbo_1204"/>
    <dbReference type="HOGENOM" id="CLU_2504962_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="10201" checksum="22D88B87E20DD877" modified="2011-05-03" version="1">MDEKEFEAMEKEFKTKEIEIIIGDNILDEDILIFDGRKYKIEDMSYNDGNLEKIKVTPIMTDKEFADWLKYKRLKYKSKLKGITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6F9</accession>
    <name>F0T6F9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645944.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10593">
        <property type="protein sequence ID" value="ADZ10593"/>
        <property type="gene ID" value="Metbo_2380"/>
    </dbReference>
    <dbReference type="GeneID" id="10278846"/>
    <dbReference type="KEGG" id="mel:Metbo_2380"/>
    <dbReference type="eggNOG" id="arCOG07976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485894_0_0_2"/>
    <dbReference type="OrthoDB" id="98713at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="181" mass="21178" checksum="1AC5B35952206C7C" modified="2011-05-03" version="1">MKKSHNQSLDQYWKLDDQQLNTPDHDAMLLWILKPGNLKKLITSRSSESENWDWKNVKIHAEEYIVSNSGFRVGAPDIIFLVPHYICKTCKTTHNDTNSCPNCNSEDIAPHPDHEHHYDRYFVEIKPKIENFGTTLRQLKLFISYGYDDRAYLFTKDLRFKAEFEGQGIKVLHQSSVQSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G7</accession>
    <name>F0T6G7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps10</name>
        <name evidence="6" type="ordered locus">Metbo_2388</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645952.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2388"/>
    <dbReference type="EnsemblBacteria" id="ADZ10601">
        <property type="protein sequence ID" value="ADZ10601"/>
        <property type="gene ID" value="Metbo_2388"/>
    </dbReference>
    <dbReference type="GeneID" id="30411126"/>
    <dbReference type="KEGG" id="mel:Metbo_2388"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="107993at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_S10_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_S10" evidence="5">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11556" checksum="C7D240DFE6323FF4" modified="2011-05-03" version="1">MHKARIKLTGTDPEKLAYVCDQLKRIAERTGVDLSGPIPLPTKKLVVPTRKSPDGEGKATWEKWELRIHKRLVGIEADERAMRQVMKVNVPDNVSIEIELKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I2</accession>
    <name>F0T6I2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645967.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2403"/>
    <dbReference type="EnsemblBacteria" id="ADZ10616">
        <property type="protein sequence ID" value="ADZ10616"/>
        <property type="gene ID" value="Metbo_2403"/>
    </dbReference>
    <dbReference type="GeneID" id="10278875"/>
    <dbReference type="KEGG" id="mel:Metbo_2403"/>
    <dbReference type="eggNOG" id="arCOG06485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050671_0_1_2"/>
    <dbReference type="OMA" id="ILSCAMV"/>
    <dbReference type="OrthoDB" id="58049at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009343">
        <property type="entry name" value="DUF1002"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06207">
        <property type="entry name" value="DUF1002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="294" mass="31582" checksum="2B85A6D4AE8DB855" modified="2011-05-03" version="1" precursor="true">MKKYLVGFIFVLMILSPIYAATGFAITYGETTYGNSNWKNSMDTYFQTKTTKNVSDAATKVVTASEVNAISSNITGKTYPASQIYSCAMVDLSYNKGINVSVDTSKITVVTPKMYENALKSTGISNGYVVVSSPVSASGEAALAGVLRSYEIAVGTPIPDQAKKAATQELYTETQIANQTGQNPDQISNLFSNVTDQIQAQNITDTNQILVIVNNVAATMNINLTQDQAQQIANSLADSQNAQASLTDFKKQLQNVSNQASQSGGILDQIMSFVQGIINYVSNLISDSQVAHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J3</accession>
    <name>F0T6J3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitrogenase</fullName>
            <ecNumber evidence="4">1.18.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645978.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2414"/>
    <dbReference type="EnsemblBacteria" id="ADZ10627">
        <property type="protein sequence ID" value="ADZ10627"/>
        <property type="gene ID" value="Metbo_2414"/>
    </dbReference>
    <dbReference type="GeneID" id="10278886"/>
    <dbReference type="KEGG" id="mel:Metbo_2414"/>
    <dbReference type="eggNOG" id="arCOG00594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_2_0_2"/>
    <dbReference type="OMA" id="GLNNMID"/>
    <dbReference type="OrthoDB" id="15283at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1 2" id="KW-0535">Nitrogen fixation</keyword>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="21"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="460" mass="50617" checksum="BBA5E9E9F9239B37" modified="2011-05-03" version="1">MSCVNVIEKDRNVIINPLKTCQPLGAMFAVTGINKGFPIVHGSQGCSAFVRYGFARHFREPSEIAVTALHEDAAVFGGRTNLVSGIENLALRFDPDVIGIITTCSSEIIGDDVYGFADTARGNLKEKLGEAADKIEIIPINTPSFVESHYKGYDNAINALVNHLARKDEPNEKVNIIPGIINPGDIRELKHMLASMDTDGIFLTDISDPFDAPLRPSKTKEIPYYPKGGTTIDELRDSANSLGTVSICKYAGSGATSLEREHKVPSAIETPPIGIQNTDQFVRNVSKFTGNSVSEELLDERGMLVDSMADNAARYLYGRKVAIYGDPDLTTGMARFVGELGMEPTMVCTGVKYPDFNPDMEKISKESGSDIDVLYGQDLRAVEVYLKENPVDLLIGNSDGRLIALDLDIPLIRMGFPVYDRVGYQRQSVLGYRGGLYLMDLITNTVLEKYYEPTHWKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6J7</accession>
    <name>F0T6J7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645982.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2418"/>
    <dbReference type="EnsemblBacteria" id="ADZ10631">
        <property type="protein sequence ID" value="ADZ10631"/>
        <property type="gene ID" value="Metbo_2418"/>
    </dbReference>
    <dbReference type="GeneID" id="10278890"/>
    <dbReference type="KEGG" id="mel:Metbo_2418"/>
    <dbReference type="HOGENOM" id="CLU_2519779_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="84" mass="9527" checksum="BECD64DDCE33225B" modified="2011-05-03" version="1">MVKKIAEYGKTELERSKTKTGPTEATAHFHIKNSMVRGSMLQIKSKENLLVENLQTKIEKSNIVEKNIYKGVVEYLECINLSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K5</accession>
    <name>F0T6K5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RcpC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645990.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2426"/>
    <dbReference type="EnsemblBacteria" id="ADZ10639">
        <property type="protein sequence ID" value="ADZ10639"/>
        <property type="gene ID" value="Metbo_2426"/>
    </dbReference>
    <dbReference type="GeneID" id="10278898"/>
    <dbReference type="KEGG" id="mel:Metbo_2426"/>
    <dbReference type="eggNOG" id="arCOG03421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039260_0_0_2"/>
    <dbReference type="OMA" id="TNLMALP"/>
    <dbReference type="OrthoDB" id="87187at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RcpC" evidence="3">
        <location>
            <begin position="291"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="496" mass="52840" checksum="255136D37BBE1CA0" modified="2011-05-03" version="1">MLDKILGKKDKDKNDTPDLRKNGKKPDSDNSDIGGRLKDMVGKVSGKPKDDDTDTSKSDSKLNSSSKPKIVSKKPAAERKMPRPITKPTSKPIDSKPRLRTPEKKKSGGLAGIGGGFGKKLPEDDQRTLVGAAVFGIILIILVGAGYYFLVYGPYQDTLNSAKMAKINEVNSYFKGALSADPHKTLLLADIDSGQTPDQVLAVDVIGPATSAWRDYQNQQINLKKDPYNRVQISYSVPSQTGGGTNGTAADTSTSSAGTSSANVKNLILNTTAAGIIVNEADASVLANMEIVTPDTVAVPVYISRIQAAGGLINVGDSVDVYQNVNTSQTTSSGNNTTQAQPTTTTTTTSSPEISGATVLAILRDTSNGIYTSTDANLTHQQQFSVNGAGVSTLTSQSAQGNVEALLKAGASRNWNEAQITSLLNAYGWRLSDFERVSNLGDLTNNYLVVLEVPRENALWVIQNDNVVAGSSLYLVVPTQRAPEWMITELHQIYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6K8</accession>
    <name>F0T6K8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645993.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2429"/>
    <dbReference type="EnsemblBacteria" id="ADZ10642">
        <property type="protein sequence ID" value="ADZ10642"/>
        <property type="gene ID" value="Metbo_2429"/>
    </dbReference>
    <dbReference type="GeneID" id="10278901"/>
    <dbReference type="KEGG" id="mel:Metbo_2429"/>
    <dbReference type="eggNOG" id="arCOG06484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085609_0_0_2"/>
    <dbReference type="OMA" id="WCVIAGN"/>
    <dbReference type="OrthoDB" id="87109at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="209" mass="23846" checksum="476D8886F558E3C3" modified="2011-05-03" version="1">MIENKIKTLRQAAKVSTMQESDEIWCVIAGNEEMVNNIAYAAITDKDRVKILRREHINSKESFVTKKFDFILLHGDINKIRDLSLLCKENGGAYLKIAPYYVLNEPNLILSVGPENSIKKFMGDCEKSRLNINFLIEDKTTGFIETDVYITNVLPDFVRSTIDPLFKMADVALSTVLISAEEEVIPKIKEIAKVNKIFVIEFNKILKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6P5</accession>
    <name>F0T6P5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08278">
        <property type="protein sequence ID" value="ADZ08278"/>
        <property type="gene ID" value="Metbo_0025"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0025"/>
    <dbReference type="eggNOG" id="arCOG12082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392927_0_0_2"/>
    <dbReference type="OMA" id="MDENHER"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11697" checksum="4FDD93E9FA72B440" modified="2011-05-03" version="1">MVRELADIVVIVSIISSISSIILAIFAILFSMRVEGRLKKNFLRLKDIMDNNHERTKEVLANMDSEAYEIKTTIYESQKELKETLKDIMDDCDISVKKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7E3</accession>
    <name>F0T7E3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">Metbo_0119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643723.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0119"/>
    <dbReference type="EnsemblBacteria" id="ADZ08372">
        <property type="protein sequence ID" value="ADZ08372"/>
        <property type="gene ID" value="Metbo_0119"/>
    </dbReference>
    <dbReference type="GeneID" id="10276544"/>
    <dbReference type="KEGG" id="mel:Metbo_0119"/>
    <dbReference type="eggNOG" id="arCOG02268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_2_2"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="20185at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="44"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="290"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="282"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="428" mass="47431" checksum="939CC5331BEE5701" modified="2011-05-03" version="1">MDFDLKTNEKGNLKIGEADAVDLAERFGTPLYVIDENRIRDNYKRLYNAFKDYYPNFKMFYACKANTNLAVMKILENEGSGIDAVSPGEIYTSLLAGFEPERILYTGNNVTDEELKFAVESGVRLNLDSISQLKRLSKIPEAKGMEISFRVNPMVGAGHHDHCITGGPLSKFGIKEEEAVEVYKMAIELGFKPVGIHSHIGSGILDPEPFMLAVRTLMDTAGKVHKEAGVEFDFIDFGGGIGIPYTPEESKLDINDFAEKIVDLYKAKLEEYDLGTPTMCIEPGRYLVGDASVLLTKVNTIKQSYRKFAGVDAGFNTLLRPTMYGSYHHIVSASTPLAESTQTIDIAGDVCESGDLFARERPMPEIEEGDILAILNAGAYAFSMASQYNSRPKPAEVLVNNGETDVIRERETFADLFNKQNVPVRLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F9</accession>
    <name>F0T7F9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Precorrin-2 C20-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.151</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.151" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643739.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0135"/>
    <dbReference type="EnsemblBacteria" id="ADZ08388">
        <property type="protein sequence ID" value="ADZ08388"/>
        <property type="gene ID" value="Metbo_0135"/>
    </dbReference>
    <dbReference type="GeneID" id="10276561"/>
    <dbReference type="KEGG" id="mel:Metbo_0135"/>
    <dbReference type="eggNOG" id="arCOG00648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_1_2"/>
    <dbReference type="OMA" id="MEYPVTT"/>
    <dbReference type="OrthoDB" id="83180at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043781">
        <property type="term" value="F:cobalt-factor II C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="235" mass="26328" checksum="296FD97466AF448B" modified="2011-05-03" version="1">MKKGKLYGIGVGPGDTELLTIKASKLLKTVDVICSPRSAESKPSIALTIIQPVLDEREDEYEIVDPLFPMIEDKSELNLYWDHAADRIERRLEKGLNVAFITLGDPTIFSTFAYVADRISKLGFEIEVVPGITSFTGCAAASGISLVEKDEILVVVPKVDDRLEKILPHADTFVIMKTSRHSEDLERIIEADEREKTIFSVQNCTMPDENIFKGFSKDKKYLTTTLVKFSPDKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H5</accession>
    <name>F0T7H5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1301</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644894.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09543">
        <property type="protein sequence ID" value="ADZ09543"/>
        <property type="gene ID" value="Metbo_1301"/>
    </dbReference>
    <dbReference type="GeneID" id="10277752"/>
    <dbReference type="KEGG" id="mel:Metbo_1301"/>
    <dbReference type="eggNOG" id="arCOG02685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_2"/>
    <dbReference type="OMA" id="WAITASF"/>
    <dbReference type="OrthoDB" id="38526at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="5">
        <location>
            <begin position="17"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="453" mass="48875" checksum="23D71A96DF55A941" modified="2011-05-03" version="1" precursor="true">MLFNKNFKIINIFVILVAAITSIGGLLFGYDTGVISGAILFIREDFLLSTTAQEVTVSAVLIGAVIGASISGILADRYGRKIMIVLASIIFGIGAIFSSVSPNVNALIISRVVVGIAIGMASFIVPLYIAEVAPINIRGALVSLNQLAITLGIVISYMVDLYFAPNGSWRWMLGLAVIPSLILALGMFFMPPSPRWLISKGFESKAVAVLKKIRGIDNVDKEVNEIEQTLLLENEGKWSDLLEPKIRSALIIGIGLAAFQQLTGINTVIYYAPTILEFAGLQTATVTIFATVGIGVVNVLLTVVSILLIDRLGRRPLLLAGITGMIVSLGIMGLAFIIPGLTSSLGWLAVICLMLYVGSFAISLGPIFWLMIAEIYPLRIRGRAMSIVTMINWATNLVVAITFLTIIELLGASGTFWLYGVIAVLSLLFVYYRVPETKGKSLEEIERLCIGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K3</accession>
    <name>F0T7K3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644922.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09571">
        <property type="protein sequence ID" value="ADZ09571"/>
        <property type="gene ID" value="Metbo_1330"/>
    </dbReference>
    <dbReference type="GeneID" id="24964551"/>
    <dbReference type="KEGG" id="mel:Metbo_1330"/>
    <dbReference type="eggNOG" id="arCOG03226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792131_0_0_2"/>
    <dbReference type="OMA" id="PHNFHEL"/>
    <dbReference type="OrthoDB" id="117273at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18708" checksum="ACA45ECDDD4A62A9" modified="2011-05-03" version="1">MYKIFVFNGGVYRFEELEEFVEDSGGLILRRDDFHVSRGVYFISQEVHVVIIMPEEAVHDLNLLATEIKGDIELIEVDYEDKINLVSLLPIYNILSRKGNWTSIQTIEEILECPCVDGVCQEFEKTSCIDDIKKTLEALSRMEIAESRVKDGNDEFRLKPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Q7</accession>
    <name>F0T7Q7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="8">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lysS</name>
        <name evidence="10" type="ordered locus">Metbo_2526</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646090.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2526"/>
    <dbReference type="EnsemblBacteria" id="ADZ10739">
        <property type="protein sequence ID" value="ADZ10739"/>
        <property type="gene ID" value="Metbo_2526"/>
    </dbReference>
    <dbReference type="GeneID" id="10279000"/>
    <dbReference type="KEGG" id="mel:Metbo_2526"/>
    <dbReference type="eggNOG" id="arCOG00485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_1_0_2"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="OrthoDB" id="9880at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anticodon_2" evidence="9">
        <location>
            <begin position="440"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="525" mass="61363" checksum="DD5744C7C6183552" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R3</accession>
    <name>F0T7R3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA polymerase II large subunit</fullName>
            <shortName evidence="11">Pol II</shortName>
            <ecNumber evidence="11">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="11">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">polC</name>
        <name evidence="13" type="ordered locus">Metbo_2532</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="11"/>
    <dbReference type="EC" id="3.1.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646096.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2532"/>
    <dbReference type="EnsemblBacteria" id="ADZ10745">
        <property type="protein sequence ID" value="ADZ10745"/>
        <property type="gene ID" value="Metbo_2532"/>
    </dbReference>
    <dbReference type="GeneID" id="10279006"/>
    <dbReference type="KEGG" id="mel:Metbo_2532"/>
    <dbReference type="eggNOG" id="arCOG04447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001154_0_0_2"/>
    <dbReference type="OMA" id="KRRNCDG"/>
    <dbReference type="OrthoDB" id="559at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="PTHR42210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolC_DP2" evidence="12">
        <location>
            <begin position="3"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1108" mass="123453" checksum="FBCDEEA2AE6253C9" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y4</accession>
    <name>F0T7Y4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643822.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0219"/>
    <dbReference type="EnsemblBacteria" id="ADZ08471">
        <property type="protein sequence ID" value="ADZ08471"/>
        <property type="gene ID" value="Metbo_0219"/>
    </dbReference>
    <dbReference type="GeneID" id="10276647"/>
    <dbReference type="KEGG" id="mel:Metbo_0219"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="OMA" id="CQKKYVS"/>
    <dbReference type="OrthoDB" id="122958at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="156" mass="17052" checksum="3CAB16CFCDA3F0AC" modified="2011-05-03" version="1">MPYEAEILIVGCGNILFQDDGFGPAVIDAIDKLSEEKPLPDNTMTIDAGTGGPHFVFSLPHETWKKMIVVDIVDFGAEPGTLRVFDVEELPKGAYENMHSWPVNQPLHDLSKVCEVMVIGVQPESVSAPNIELGLTESVENAIPKAIEIILKEIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T849</accession>
    <name>F0T849_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645026.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1437"/>
    <dbReference type="EnsemblBacteria" id="ADZ09675">
        <property type="protein sequence ID" value="ADZ09675"/>
        <property type="gene ID" value="Metbo_1437"/>
    </dbReference>
    <dbReference type="GeneID" id="10277888"/>
    <dbReference type="KEGG" id="mel:Metbo_1437"/>
    <dbReference type="eggNOG" id="arCOG02575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_2_0_2"/>
    <dbReference type="OMA" id="FACKLKG"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="24402" checksum="8F4E8150D4480E74" modified="2011-05-03" version="1">MKIIGINGSPRKEWNTAELIKKALEGAKSEGAETELIHLYDLEYKGCKSCFACKTKGSKSYGRCNVKDDLTAVFEMIEAADALILGSPIYFGVVTGEMRSFMERMIFPCFTYTTPPKSLFPKQISSAFLYTMNIPEEMIEDYGYKMQFESNKQLLTLLFGSSEALMSYDTYQFKDYSKVVADRFDPEHKAKHRDKQFPVDKEAAFNLGIRMAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E9</accession>
    <name>F0T8E9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645051.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1463"/>
    <dbReference type="EnsemblBacteria" id="ADZ09700">
        <property type="protein sequence ID" value="ADZ09700"/>
        <property type="gene ID" value="Metbo_1463"/>
    </dbReference>
    <dbReference type="GeneID" id="10277914"/>
    <dbReference type="KEGG" id="mel:Metbo_1463"/>
    <dbReference type="HOGENOM" id="CLU_132137_0_0_2"/>
    <dbReference type="OrthoDB" id="100931at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018658">
        <property type="entry name" value="DUF2089"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024064">
        <property type="entry name" value="FdhE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09862">
        <property type="entry name" value="DUF2089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144020">
        <property type="entry name" value="SSF144020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13734" checksum="61D625A33D60ABCF" modified="2011-05-03" version="1">MKREVPGHCPICGGETKVTEIHCRKCDTTIQGKFDLCKFCKFTEQQKYFVEVFIKNRGNIKEIEKELGISYPTVRNKLDEVIHALGHKVEKQTVNQKEILEKLSKGEISKNEALKLLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F3</accession>
    <name>F0T8F3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SOUL heme-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645055.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09704">
        <property type="protein sequence ID" value="ADZ09704"/>
        <property type="gene ID" value="Metbo_1468"/>
    </dbReference>
    <dbReference type="GeneID" id="10277919"/>
    <dbReference type="KEGG" id="mel:Metbo_1468"/>
    <dbReference type="eggNOG" id="arCOG04820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068699_0_1_2"/>
    <dbReference type="OrthoDB" id="96329at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_haem-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="PTHR11220"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="23058" checksum="F5795B9AB1927A31" modified="2011-05-03" version="1">MVETLAYEVEKKDGDFEIRSYGDHILAHVDVEAPFDEAMSMGFKVLAHYIFGGNKKRSSIDMTAPVEEEKRNSEKIPMTSPVTEESLMESEKIKMTTPVTEEKTGNIHRISFVMPSNYTMEALPEPEDEKIKFEEIKAEKMAVLRFKGRVKENLANEKIEEMKNWLKENNIQAKSNFVVAQYNNPAVPSFFRRNEIMVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F9</accession>
    <name>F0T8F9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Daunorubicin resistance ABC transporter ATPase subunit</fullName>
            <ecNumber evidence="5">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1475</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.30" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645061.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1475"/>
    <dbReference type="EnsemblBacteria" id="ADZ09710">
        <property type="protein sequence ID" value="ADZ09710"/>
        <property type="gene ID" value="Metbo_1475"/>
    </dbReference>
    <dbReference type="GeneID" id="10277926"/>
    <dbReference type="KEGG" id="mel:Metbo_1475"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="ITAPMAD"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="323" mass="35587" checksum="040BF68F28BAB24F" modified="2011-05-03" version="1">MTKYALELNNLSKKYGDFTAVDDLSLQVKEGEIFGFLGPNGAGKSTTIRMLCTLALPTSGSAKIAGYDLSKDSGKVRENIGLVAEKLIMYDGLTAGQNLRFFGKLYGIPKKKLEKRIDELLELIDMKKWKDTKINTFSTGMKQRINIIRALLAEPKIIFMDEPTLGLDPQTTFSVREIIRDINSKGVTVILTTHAMVEAEALSDRVAIIDHGKIAALDTPENLKKMLSDTDITIFKMEITNLTVEMIENIKSLDVVTALSKQDEHNIKISAKGPDSLEEIIDTIRFDGGKISSIANINDATLEDVFLTVTGKEIRDTPAANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H3</accession>
    <name>F0T8H3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645075.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1491"/>
    <dbReference type="EnsemblBacteria" id="ADZ09724">
        <property type="protein sequence ID" value="ADZ09724"/>
        <property type="gene ID" value="Metbo_1491"/>
    </dbReference>
    <dbReference type="GeneID" id="10277942"/>
    <dbReference type="KEGG" id="mel:Metbo_1491"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_1_2"/>
    <dbReference type="OrthoDB" id="55420at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="22"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="246" mass="27533" checksum="CFA327C41A35270E" modified="2011-05-03" version="1">MKIADKIEMLEVPMKLMGTHGTIYPTVIWDDEHVVHIDAGISDSKSLIKDEMDKAGVPFDKIDTILVTHQDIDHICGINCIVDELGDVKVLAHSEEKSYIQGEKPILRFKNSNIKERIKMLPEDEQQKVLDMVENTSVKVDGELSDGDKLDYGGGIVVIHTPGHTPGHICLYHEESKSLIVGDALNIEGNELVVTHRDAMSKKELIIINGWLEKLGEFDIENVIAYHTGLFKNDPNQMLKELSNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L3</accession>
    <name>F0T8L3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription elongation factor NusA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643906.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0303"/>
    <dbReference type="EnsemblBacteria" id="ADZ08555">
        <property type="protein sequence ID" value="ADZ08555"/>
        <property type="gene ID" value="Metbo_0303"/>
    </dbReference>
    <dbReference type="GeneID" id="10276735"/>
    <dbReference type="KEGG" id="mel:Metbo_0303"/>
    <dbReference type="eggNOG" id="arCOG01761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132460_0_0_2"/>
    <dbReference type="OMA" id="MKAPICE"/>
    <dbReference type="OrthoDB" id="82272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH_5" evidence="6">
        <location>
            <begin position="57"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="165" mass="18060" checksum="78E4F848584B978C" modified="2011-05-03" version="1">MVLPVCDVCLKSGILCQGCENKLESGEISQIDLDIAKVLFKIGDGKIGFKKTIEVGDIVIIVTDQDQVGKLIGKNGKIVRELSRTVGKKIRVVGQDSDLKSVSKDILSPARVSGINVVYGKDGDDKYKIRIVQEDSRRLPGRLDVLNEIIELLTGEKTVLVVDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P6</accession>
    <name>F0T8P6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine--scyllo-inositol transaminase</fullName>
            <ecNumber evidence="2">2.6.1.50</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.50" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643939.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0336"/>
    <dbReference type="EnsemblBacteria" id="ADZ08588">
        <property type="protein sequence ID" value="ADZ08588"/>
        <property type="gene ID" value="Metbo_0336"/>
    </dbReference>
    <dbReference type="GeneID" id="10276773"/>
    <dbReference type="KEGG" id="mel:Metbo_0336"/>
    <dbReference type="eggNOG" id="arCOG00118">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_2"/>
    <dbReference type="OMA" id="IVNHGMY"/>
    <dbReference type="OrthoDB" id="37018at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047310">
        <property type="term" value="F:glutamine-scyllo-inositol transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080100">
        <property type="term" value="F:L-glutamine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="364" mass="39657" checksum="39FA597F719E971C" modified="2011-05-03" version="1">MIPIAKPIIADEEIEEVVNVLKSGFIAQGPKVAEFEAKFADFVGVTQAAASSSGTTALHLALLAAGVGPEDEVITTPFTFAATGNSVLYVGAKPVFVDIDRDTYNLDPEKIEEAITEKTKVIIPVHLYGQPADMDPINKIAKDNGLTVIEDAAQAHGAVYKGKKAGSIGDMGCFSFYPTKNMTTSEGGMVTTNNAELAEMVRILRAHGESERYTHVTLGYNFRMTDIAAAIGVVQLKKLEKFNEQRIKNAKYLSEKIDGIEGIKPPYVSPDVKHVFHQYTIRVDKSKRDELKEFLNENGVGTGIHYPKPIYQQKLYMELGFTASCPEAESASLEVLSLPVHPSLETEDLDTIVSVLEAASSKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T6</accession>
    <name>F0T8T6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPase, P-type (Transporting), HAD superfamily, subfamily IC</fullName>
            <ecNumber evidence="9">3.6.3.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1534</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.8" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645115.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1534"/>
    <dbReference type="EnsemblBacteria" id="ADZ09764">
        <property type="protein sequence ID" value="ADZ09764"/>
        <property type="gene ID" value="Metbo_1534"/>
    </dbReference>
    <dbReference type="GeneID" id="10277989"/>
    <dbReference type="KEGG" id="mel:Metbo_1534"/>
    <dbReference type="eggNOG" id="arCOG01578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_0_2"/>
    <dbReference type="OMA" id="CYIAYSV"/>
    <dbReference type="OrthoDB" id="17967at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="765"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="811"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="851"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="912" mass="100775" checksum="1FDAB67A6EB7DCFA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V7</accession>
    <name>F0T8V7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">AL-21</strain>
        </source>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        </source>
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    <evidence type="ECO:0000313" key="1">
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        </source>
    </evidence>
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    </evidence>
    <sequence length="185" mass="21414" checksum="338571B656E4F7F7" modified="2011-05-03" version="1">MIKKDIIIGVTMVDDEKLIEKIYQSILNLELEFQIYVKGQNGLNNIVLFDEPELYPREKARFRIGNGSILIFDGEKPILAIEIISNKPTSSKEIAGPIPVYMISRKIKLNYKDGTEREIDLLNKFPLLVVVPDQSKTKTNQINDLNEKFRGIFKLNTEYSALNYFRVCVESDFDYYLKKLLSIEV</sequence>
</entry>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="entry name" value="PDDEXK_7"/>
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    <dbReference type="Pfam" id="PF09823">
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            <end position="365"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF09823"/>
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    </evidence>
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            <dbReference type="EMBL" id="ADZ09787.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="588" mass="70165" checksum="772F233990D20EF3" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Z2</accession>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ09820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645171.1">
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    <dbReference type="STRING" id="877455.Metbo_1593"/>
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        <property type="gene ID" value="Metbo_1593"/>
    </dbReference>
    <dbReference type="GeneID" id="24964567"/>
    <dbReference type="KEGG" id="mel:Metbo_1593"/>
    <dbReference type="eggNOG" id="arCOG02335">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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            <end position="281"/>
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        <location>
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            <end position="336"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="664" mass="75530" checksum="1B5E4AAB2594C93F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T903</accession>
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        <submittedName>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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                <person name="Zinder S.H."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09831">
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        <property type="gene ID" value="Metbo_1604"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1604"/>
    <dbReference type="eggNOG" id="arCOG02889">
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    <dbReference type="OMA" id="SILCTRI"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="30078" checksum="50FB7233022DCCC2" modified="2011-05-03" version="1" precursor="true">MIKYGFTVLLLVLSCFMQIGFAQSIDGNNGKMMLIYVGTDYYNPQTMTGGEVTHNQYIKDHVANTDISNSVCRLAKFGTPMIILGNGSSPKVMITAGVHGNEIPAQLAAMELINYLKGREINGTVYVVPFVSPNGTSQTLRYWNHQNLNSNANKEGTPSNAVILACKQLGIERLGDFHSTQPGGYPGNYSVLCSKSPEYESYVMAEYISSYSNSSLITYDLAGVDYEGAVEDVSNIQGIPAVTCEVLSPQGVATEETVHRSFDQMIKFLEYCKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T912</accession>
    <name>F0T912_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fe-S type hydro-lyase tartrate/fumarate beta region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0382</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643985.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0382"/>
    <dbReference type="EnsemblBacteria" id="ADZ08634">
        <property type="protein sequence ID" value="ADZ08634"/>
        <property type="gene ID" value="Metbo_0382"/>
    </dbReference>
    <dbReference type="GeneID" id="10276819"/>
    <dbReference type="KEGG" id="mel:Metbo_0382"/>
    <dbReference type="eggNOG" id="arCOG04406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098588_2_0_2"/>
    <dbReference type="OMA" id="VIMHTAV"/>
    <dbReference type="OrthoDB" id="81998at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="167" mass="17927" checksum="4F7477E3B944F5AA" modified="2011-05-03" version="1">MKDVVELRTPITLKNIREIVVGDWLELSGVILTGRDAALPKLVSSIKNGQPAVNIQGMVIMHTAVSDAGISPTTSNKEEIEESIPFLSQEGVRIHLGKGALSEKTVDALNQFNSIYAVTPPAAALLTSKVKNRKVAAFEEEGMEAIHALEVSRLPCIVASAHGKTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T932</accession>
    <name>F0T932_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Abhydrolase_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644005.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0402"/>
    <dbReference type="EnsemblBacteria" id="ADZ08654">
        <property type="protein sequence ID" value="ADZ08654"/>
        <property type="gene ID" value="Metbo_0402"/>
    </dbReference>
    <dbReference type="GeneID" id="10276839"/>
    <dbReference type="KEGG" id="mel:Metbo_0402"/>
    <dbReference type="eggNOG" id="arCOG01654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077889_0_0_2"/>
    <dbReference type="OMA" id="QFTYKPT"/>
    <dbReference type="OrthoDB" id="72537at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029059">
        <property type="entry name" value="AB_hydrolase_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12695">
        <property type="entry name" value="Abhydrolase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_5" evidence="1">
        <location>
            <begin position="71"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="27203" checksum="25BC547DCFDCCD0A" modified="2011-05-03" version="1" precursor="true">MKYINSKKRLILLILLLVVVVAVAGFSYYVSDYYHADSNATEALSSNNGYSVINNSDSITFIPTNNKTTTGIIFYPGAKVQPEAYSVIASKLAQNGYTTIIVKMPFNLAIFGVDKANDVINQHQDIKTWVIGGHSLGGVFASDFAVKHQDKIKGVIYLAAYPNTNASNATFKALSIRGSLDGLATQDQITKNLDKFPKNTTIITIDGGNHYNFGDYGVQSGDNNSTITRQEQQNQTVTAIINFIKTLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T983</accession>
    <name>F0T983_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0677 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645185.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1607"/>
    <dbReference type="EnsemblBacteria" id="ADZ09834">
        <property type="protein sequence ID" value="ADZ09834"/>
        <property type="gene ID" value="Metbo_1607"/>
    </dbReference>
    <dbReference type="GeneID" id="10278064"/>
    <dbReference type="KEGG" id="mel:Metbo_1607"/>
    <dbReference type="eggNOG" id="arCOG03588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056160_3_1_2"/>
    <dbReference type="OMA" id="IWEGVTQ"/>
    <dbReference type="OrthoDB" id="54231at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011610">
        <property type="entry name" value="CHP00027_methylltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00027">
        <property type="entry name" value="mthyl_TIGR00027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="288" mass="33464" checksum="00528429051B650E" modified="2011-05-03" version="1">MTKEAAKTAMGPTFMIAVEQSFPEEQRIITDELAYPILPLSYRLMVKLFNIKFVRHWLIGTTETSTPGLWSGIMLRKLYIQHKINTSQSEIGQVVNLGSGYDTMAYTLPSLSDMSVWELDQKNVIETKTQRLETSMGEVPGNVKLMAMDFDHEDIGQALKRQGYSDEMPTFFILEAVTQYLDEESVKKMFTFLSQAQTGSKLIFTYILREFIEGVEMYEMKNLYKKYVFPKIWKYGLFTQDTKEFLEGYGWKLIEDVEADDINDDYIKPNNRNLKTTNLERIVYAEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9D1</accession>
    <name>F0T9D1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09882">
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        <property type="gene ID" value="Metbo_1656"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3178575_0_0_2"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="46" mass="5161" checksum="EE3703FDF6A3151D" modified="2011-05-03" version="1">MAITAKLNIEKTYVKIENSCTGTFLLLNKEEAVKLMDDLAVVTEKM</sequence>
</entry>
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    <accession>F0T9F3</accession>
    <name>F0T9F3_METLA</name>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="14795" checksum="A85CC5B988CDB32D" modified="2011-05-03" version="1">MEGVLVVMNQEMMDNITKNNLVFLATAAADGTPNVVPIGLAKPLDNKTVLLVANFMKKTIQNLKNNPKASVIVGNVSECPYQFKGSVKIEESGKNYDDAVEWAKSVMAQLNPFAAVILEVEEVYSVQPGPDAGKLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9K0</accession>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADZ08748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="877455.Metbo_0496"/>
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        <property type="protein sequence ID" value="ADZ08748"/>
        <property type="gene ID" value="Metbo_0496"/>
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    <dbReference type="GeneID" id="10276936"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="149"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="149" mass="16728" checksum="334132640A7971BC" modified="2011-05-03" version="1" precursor="true">MPTFNQKMLIILAIVVVGVLIIFAVTNLEKTSKTQNSNQDNPQAIQWNYNLDSAINTAQKSNKLIFVDFYADWCGYCKQLDEKTYPNGKVEAVMAQKYVAVKVNVDQNPDLASKYSVYGLPTLVIMDSNGNEIKRVEGYQTPSDLLNIL</sequence>
</entry>
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    <accession>F0T9L1</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="HATPase_C"/>
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        <property type="entry name" value="His_kinase_dom"/>
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        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="121"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="259"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="454" mass="52021" checksum="6518AFC9A72DF200" modified="2011-05-03" version="1">MVKIMTRNPNHELYKLITISSLLIISCLLTYYFHFVIGEGVIFTHFFYFPIILAALWWQKKGLIVPIFLTLMLLVSYYLSPNLSYPLYEDIYRSIIFLSIGIVVAILSDVINKKDKELSASEEKFRSVANSAVDGIITTNQNGDIVLMNPSLKRIFGYDLDEIKGRNVTMLMPERYKKDFETKLQNFNSTGTHELDGKTFESIGLKKNGEEFPFEISVATWGYKTGPYTTSIIRDVADRKLTEHQLQKSIKEKEMLLKEIHHRVKNNLMIISSLLSLQSRYIKDENSKTIFIESQNRARSMALIHERLYQSTDLKNIDFGDYIQTLAADLYDTYVVDKNLIKMNIETDELNLDIDTSIPLGLILNELVTNSLKHAFSQGDKGTIDIKFKKQTDKYCLEVKDNGKGFPNDIDYKNADSLGLSLITSLSEQIDADLEYIGSPGTSFKISFNEKKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M3</accession>
    <name>F0T9M3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644122.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0519"/>
    <dbReference type="EnsemblBacteria" id="ADZ08771">
        <property type="protein sequence ID" value="ADZ08771"/>
        <property type="gene ID" value="Metbo_0519"/>
    </dbReference>
    <dbReference type="GeneID" id="10276964"/>
    <dbReference type="KEGG" id="mel:Metbo_0519"/>
    <dbReference type="eggNOG" id="arCOG01861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056349_2_0_2"/>
    <dbReference type="OMA" id="NLVGHLV"/>
    <dbReference type="OrthoDB" id="32644at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="164"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="297" mass="33485" checksum="F149D7E8887F0041" modified="2011-05-03" version="1">MPKNEKDFIKNLNVTRSVRTKFAVSDKLIKTSASGNQYLDVTLTDKTGQFDGRIFQDVEEIYNKINVGSVCLVKGRINEFPSGSGRFNMVINSITELDEADYQNEDFVRVSENDVKESLELITTTIDDMDNQDLKNLLNSFFADEEFAEQFSKAPAAIVHHHNYEGGLLDHTIEVLKLCKTTTELFPNLDWELLYTGVLLHDLGKMKTYTYGNGIIGYSSEGNMLDHIYLSCEMVQEKLNSLEIPRELALKILHMILSHHGDVNLGWGSSVDPKTPEALALHYADNLDAKVKKSLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M7</accession>
    <name>F0T9M7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polymorphic outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1680"/>
    <dbReference type="EnsemblBacteria" id="ADZ09906">
        <property type="protein sequence ID" value="ADZ09906"/>
        <property type="gene ID" value="Metbo_1680"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1680"/>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="466"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="563"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="666" mass="70895" checksum="F65B2DDC8D552D95" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N3</accession>
    <name>F0T9N3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1687</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645263.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1687"/>
    <dbReference type="EnsemblBacteria" id="ADZ09912">
        <property type="protein sequence ID" value="ADZ09912"/>
        <property type="gene ID" value="Metbo_1687"/>
    </dbReference>
    <dbReference type="GeneID" id="10278144"/>
    <dbReference type="KEGG" id="mel:Metbo_1687"/>
    <dbReference type="eggNOG" id="arCOG02497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441896_0_0_2"/>
    <dbReference type="OMA" id="NEAYSIK"/>
    <dbReference type="OrthoDB" id="11227at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="595"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="NosD" evidence="3">
        <location>
            <begin position="119"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="618" mass="65735" checksum="84FA3CC7080BCDD3" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Q8</accession>
    <name>F0T9Q8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1713"/>
    <dbReference type="EnsemblBacteria" id="ADZ09937">
        <property type="protein sequence ID" value="ADZ09937"/>
        <property type="gene ID" value="Metbo_1713"/>
    </dbReference>
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    <dbReference type="OMA" id="KDERESH"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="212" mass="23793" checksum="E20DC51FA79F747C" modified="2011-05-03" version="1">MKSLNYRMDLFGILALVVFSVLTFISLSLYPESYSIAFNWLSNLGNVILNPQGAIYFNMACIVTGIILIQFIIQFYRWDTGNLFERILLALAIVLGIFASVSLIFVGVFPETDINLHVLAAKGVFEALFLSIILMTAAIFNHPKFMIGVALIGVMAVIIDLFFLTLLSLPKYHDALATPYPTLPIPGLEWFAVFLSFIWLAALSYNMYKMKI</sequence>
</entry>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="Metbo_1715"/>
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    <dbReference type="OrthoDB" id="24323at2157"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="634" mass="71261" checksum="9BCF3BC61B4E4E72" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R6</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="3" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1721"/>
    <dbReference type="EnsemblBacteria" id="ADZ09945">
        <property type="protein sequence ID" value="ADZ09945"/>
        <property type="gene ID" value="Metbo_1721"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG01994">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
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        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
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        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="EMBL" id="ADZ09945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="24167" checksum="F00E2BC085416040" modified="2011-05-03" version="1">METDGTHDRNSYLNYFSGLYRRNKKLLMVSFSIYFLSVIAGIMLALFLSGPVSSYLTTMVQTDRQFLSEKGFNFLTIFSHNLFSLVLTFVGGITAVITVIFIIINGFVYGAFLGYVASNPVIGGQASSVGSLTPLKFIVYTVPHGIFEISGFIIAGAAGLRLSTIIIDIIRKDEESNYYYNQIKDALALFGIAVVLIFIAAIIETNVTLHIGNFIFQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9T2</accession>
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        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645312.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1737"/>
    <dbReference type="EnsemblBacteria" id="ADZ09961">
        <property type="protein sequence ID" value="ADZ09961"/>
        <property type="gene ID" value="Metbo_1737"/>
    </dbReference>
    <dbReference type="GeneID" id="24964574"/>
    <dbReference type="KEGG" id="mel:Metbo_1737"/>
    <dbReference type="eggNOG" id="arCOG02643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_3_2"/>
    <dbReference type="OrthoDB" id="93551at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="182" mass="21259" checksum="1739902DF81D2F96" modified="2011-05-03" version="1">MTDETKRIILDAALKIFAKEGYNSATTRNIANQSGFSEITLFRKFETKEKLFKEAMSVNHEILQETCISLMEDLEKIEDPEEFLREYIKRMAVFYHENFEFFNIMVTEDNLHVDSDMGDFGDVLSEFVSENIGNTCCEVTSIVFTINTFIYTLNLDMFHGINSDGYEENIKRFTNNMVRCLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U5</accession>
    <name>F0T9U5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="10">GAPDH</shortName>
            <ecNumber evidence="5 10 12">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gap</name>
        <name evidence="14" type="ordered locus">Metbo_1751</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10 12">
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="5 10 12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645325.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1751"/>
    <dbReference type="EnsemblBacteria" id="ADZ09974">
        <property type="protein sequence ID" value="ADZ09974"/>
        <property type="gene ID" value="Metbo_1751"/>
    </dbReference>
    <dbReference type="GeneID" id="10278208"/>
    <dbReference type="KEGG" id="mel:Metbo_1751"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OMA" id="NSRANYD"/>
    <dbReference type="OrthoDB" id="32079at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="7 10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="13">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="10">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ09974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="336" mass="36804" checksum="A5934A998A652762" modified="2011-05-03" version="1">MKYIGINGYGTIGKRVADAISLQKDMKLAGVTKRTPNFEAKMAADNGYNIYMSDPTKEQLFEEADIKVAGTIDDLQDKIDLIIDCTPEGVGAKNLKSYQRAGIKAIFQGGESHKEIGRSFNSAANYDENIGADYVRVVSCNTTGLCRTLTPIAEIAKIRKVRAVLVRRGADPSQVNKGPINSIVPNPPITPSHHGPDVQTVLKGVNIFTIALLVPTTLMHQHNIMIELDSKLEIDDVVNKFGESSRVILVDAKNGLGSTAEIMEYANDLGRLRSDLYEIAVWRESINIVDNELYYMQAVHQESDVVPENVDAVRALLELGDSNVSISKTNQSMGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9V8</accession>
    <name>F0T9V8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MCM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_0529</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644132.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0529"/>
    <dbReference type="EnsemblBacteria" id="ADZ08781">
        <property type="protein sequence ID" value="ADZ08781"/>
        <property type="gene ID" value="Metbo_0529"/>
    </dbReference>
    <dbReference type="GeneID" id="10276974"/>
    <dbReference type="KEGG" id="mel:Metbo_0529"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="OMA" id="VTPTHPF"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="6">
        <location>
            <begin position="271"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="8">
        <location>
            <begin position="541"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="7">
        <location>
            <begin position="888"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="MCM" evidence="6">
        <location>
            <begin position="901"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1173" mass="133145" checksum="1DB31D4CC55763DD" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W8</accession>
    <name>F0T9W8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0539"/>
    <dbReference type="EnsemblBacteria" id="ADZ08791">
        <property type="protein sequence ID" value="ADZ08791"/>
        <property type="gene ID" value="Metbo_0539"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0539"/>
    <dbReference type="eggNOG" id="arCOG01346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_5_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="90" mass="10361" checksum="C7CB4239C272E14E" modified="2011-05-03" version="1">MNITTPPKKELMNRSLSTITINIGKSKINDNVIDEIKRQLKENEVVKLRFSKGISAEKQNYITEIIEKSNSKLIDFRGNVAVIFKQRRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA06</accession>
    <name>F0TA06_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644180.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08829">
        <property type="protein sequence ID" value="ADZ08829"/>
        <property type="gene ID" value="Metbo_0578"/>
    </dbReference>
    <dbReference type="GeneID" id="10277023"/>
    <dbReference type="KEGG" id="mel:Metbo_0578"/>
    <dbReference type="HOGENOM" id="CLU_2645925_0_0_2"/>
    <dbReference type="OMA" id="KNESNPM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="76" mass="7733" checksum="FD1F037DE7843023" modified="2011-05-03" version="1">MDEKLVILIVVLVAVAGVVGYVIGLYQGAGNVTLNISNNSTNATTGATTQKNTVTPVKNNTPKNNTPSGNNTTSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA14</accession>
    <name>F0TA14_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaetidylinositol phosphate synthase</fullName>
            <shortName evidence="1">AIP synthase</shortName>
            <ecNumber evidence="1">2.7.8.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of archaetidylinositol phosphate (AIP) from CDP-archaeol (CDP-ArOH or CDP-2,3-bis-(O-phytanyl)-sn-glycerol) and 1L-myo-inositol 1-phosphate (IP or 1D-myo-inositol 3-phosphate). AIP is a precursor of archaetidyl-myo-inositol (AI), an ether-type inositol phospholipid ubiquitously distributed in archaea membranes and essential for glycolipid biosynthesis in archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1D-myo-inositol 3-phosphate + CDP-2,3-bis-O-(phytanyl)-sn-glycerol = CMP + H(+) + saturated 1-archaetidyl-1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:36823"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
            <dbReference type="ChEBI" id="CHEBI:74006"/>
            <dbReference type="EC" id="2.7.8.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.39" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644188.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0586"/>
    <dbReference type="EnsemblBacteria" id="ADZ08837">
        <property type="protein sequence ID" value="ADZ08837"/>
        <property type="gene ID" value="Metbo_0586"/>
    </dbReference>
    <dbReference type="GeneID" id="10277031"/>
    <dbReference type="KEGG" id="mel:Metbo_0586"/>
    <dbReference type="eggNOG" id="arCOG00670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080384_1_0_2"/>
    <dbReference type="OMA" id="DGNMARQ"/>
    <dbReference type="OrthoDB" id="115624at2157"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02242">
        <property type="entry name" value="AIP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044270">
        <property type="entry name" value="AIP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="195" mass="21548" checksum="D5D6DA05FDF79FD6" modified="2011-05-03" version="1">MLNRLRPQVKVILDPVAKKIRINPNILTIMGLLVSLISAYMFATGNLLMGGLLIALSGFVDMLDGAVARNNYQTTKFGGLLDSTADRFADAFILIGIIYGGYVNWIYGILALHASLTVSYVRARAESEGIKCNVGIAERAERLVIIMIGAFLSYFTNIQIFNMNLLGIAVVVIMILGYVTVIQRVYHSWKNLKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA23</accession>
    <name>F0TA23_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644197.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0595"/>
    <dbReference type="EnsemblBacteria" id="ADZ08846">
        <property type="protein sequence ID" value="ADZ08846"/>
        <property type="gene ID" value="Metbo_0595"/>
    </dbReference>
    <dbReference type="GeneID" id="10277040"/>
    <dbReference type="KEGG" id="mel:Metbo_0595"/>
    <dbReference type="eggNOG" id="arCOG00045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077587_0_0_2"/>
    <dbReference type="OMA" id="YHPCGRC"/>
    <dbReference type="OrthoDB" id="66950at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="363" mass="40160" checksum="D80880227DDED265" modified="2011-05-03" version="1">MKLEVDYMVGVLENAIKPIIGNGSAEISNLDLTTELGFTIERVIYDGETNDLTIIAPDRPEKSAVIGKGGWVVGKLREALQVNNIHVDAYSDMMVRIYRMELALKKINSSKKILGEDTTPIKNLKNLLNLRINNLAKFNYLSEFEEGLKQLKLEGSQKKLEMEKDHKAIVALSGGVDSSFSLIVAKLLGFNPVAVTVDPGSIILPSYFKKNINGLTSKLNVPHEYLPVNMGEVIENSLEGKIHPCGKCSKTIEDSLLEYAEKSEIPFIIFGDFLATGSQSMVRLNGFWRLNLPAMLSATKGETRSVSGYFDVESVGGYGCPLLNEVHKLHPHMRRFSIQRVLRETRAGVLEPGQALNLITKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA26</accession>
    <name>F0TA26_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S27ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ordered locus">Metbo_0598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644200.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0598"/>
    <dbReference type="EnsemblBacteria" id="ADZ08849">
        <property type="protein sequence ID" value="ADZ08849"/>
        <property type="gene ID" value="Metbo_0598"/>
    </dbReference>
    <dbReference type="GeneID" id="10277043"/>
    <dbReference type="KEGG" id="mel:Metbo_0598"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="OMA" id="GVFMAEH"/>
    <dbReference type="OrthoDB" id="123606at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_S27ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_S27a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_S27ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ribosomal_S27" evidence="7">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="54" mass="6330" checksum="B7FD7A93B5ABB6B8" modified="2011-05-03" version="1">MKKFELYEVKDNKIIRKNPECVRCSHGVFMADHGDRYACGKCGYTQFKGKENKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA87</accession>
    <name>F0TA87_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase class-I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645388.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1815"/>
    <dbReference type="EnsemblBacteria" id="ADZ10037">
        <property type="protein sequence ID" value="ADZ10037"/>
        <property type="gene ID" value="Metbo_1815"/>
    </dbReference>
    <dbReference type="GeneID" id="10278272"/>
    <dbReference type="KEGG" id="mel:Metbo_1815"/>
    <dbReference type="eggNOG" id="arCOG00090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_3_3_2"/>
    <dbReference type="OMA" id="TSMLHWH"/>
    <dbReference type="OrthoDB" id="54766at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="PTHR42695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="237" mass="27398" checksum="2B5941F1349993F3" modified="2011-05-03" version="1">MRLHYLQHVPFENPGSILRWANLNSVEVTKTLMFQDEDLPEHDDYDWLVVMGGPMNIYDHENYPWLVKEKSFIKTAIDSSKLVIGLCLGGQLITDVIGGEVTKNSHLEIGWFPLHLKTNAKSSPFFSFLPDQPTVFHWHGDTFNKLPENAELIAKSDACENQAFVYNDRVFGFQFHLETTEQIIDDLVVNCADEMVPGPYVQSKNELLNNENIKQTNEWMDTFLNKLFDRENRCDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA0</accession>
    <name>F0TAA0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1828"/>
    <dbReference type="EnsemblBacteria" id="ADZ10050">
        <property type="protein sequence ID" value="ADZ10050"/>
        <property type="gene ID" value="Metbo_1828"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1828"/>
    <dbReference type="eggNOG" id="arCOG02352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_1_2"/>
    <dbReference type="OMA" id="SHDESGM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="250" mass="28489" checksum="CC4C935104D3E2E7" modified="2011-05-03" version="1">MSDTKILVVEDDKIISLDIQRVLNAVGYEVPITVSTGEDAIKIAKELLPDLILMDISLKGNMDGIHAASQIKNLSIPIIYLTAYKNKSLIERAQETDPYGYILKPYDEYELTLTIDMALKKHEQITKMEGIIQETPLPLFFINNQHEVIFWNKAMEKLSGTPINEIIGTDNHWQSFYDSKRPCMADLLMDNKFRMMHELYSGKIDMSDDSWSAEEYFPKVYDGVMLNFSASLIKNIQGKTIGALEIVDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAA2</accession>
    <name>F0TAA2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ordered locus">Metbo_0603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ08854">
        <property type="protein sequence ID" value="ADZ08854"/>
        <property type="gene ID" value="Metbo_0603"/>
    </dbReference>
    <dbReference type="GeneID" id="10277048"/>
    <dbReference type="KEGG" id="mel:Metbo_0603"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_2_2"/>
    <dbReference type="OMA" id="DEPTFPG"/>
    <dbReference type="OrthoDB" id="103587at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="30"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="173" mass="19737" checksum="4D3F470FBA2D662F" modified="2011-05-03" version="1">MNLWKDIPTGPSVPEVIYAVVEIPKGSRNKYEYDKDMEAFALDRVLYSPFHYPAEYGIIPQTLWDDGDPMDILVIMDEATFPGCVIETRPIGIMRMIDGGDSDDKVLGVPVNDPRFTDIKDISQLPKQYLDEIAHFFTDYKKLEGKKTEVLGWESAETAFEAIKHSIDLYKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAH4</accession>
    <name>F0TAH4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0675"/>
    <dbReference type="EnsemblBacteria" id="ADZ08926">
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        <property type="gene ID" value="Metbo_0675"/>
    </dbReference>
    <dbReference type="GeneID" id="10277120"/>
    <dbReference type="KEGG" id="mel:Metbo_0675"/>
    <dbReference type="eggNOG" id="arCOG04899">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207588_0_0_2"/>
    <dbReference type="OMA" id="IPDPWVW"/>
    <dbReference type="OrthoDB" id="129057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="8005" checksum="B01CDF3A7A09A2DB" modified="2011-05-03" version="1">MVLGISDPWISAAYVGCILATLLCVVYGILNWNKGDEEEQAQISEEIKWHEKEKDMEEKELGLWDEEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK0</accession>
    <name>F0TAK0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SirA-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1850"/>
    <dbReference type="EnsemblBacteria" id="ADZ10072">
        <property type="protein sequence ID" value="ADZ10072"/>
        <property type="gene ID" value="Metbo_1850"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1850"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_1_2"/>
    <dbReference type="OMA" id="EIPMACE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="104" mass="11500" checksum="2E60B7C3889A0BBD" modified="2011-05-03" version="1">MEVLITEISCCFNNKYSIKLTSLGKKIRGDLMAEVKVDVKGETCPVPLVEMRKAIKNASEGEIIEVEGTHPASKKEIPMAVEALGSEIVDIQETNGVWKIKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL0</accession>
    <name>F0TAL0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645433.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1861"/>
    <dbReference type="EnsemblBacteria" id="ADZ10082">
        <property type="protein sequence ID" value="ADZ10082"/>
        <property type="gene ID" value="Metbo_1861"/>
    </dbReference>
    <dbReference type="GeneID" id="10278319"/>
    <dbReference type="KEGG" id="mel:Metbo_1861"/>
    <dbReference type="eggNOG" id="arCOG02469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="OMA" id="EMCLLDE"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="219" mass="24986" checksum="401AF3D99D2CD9B0" modified="2011-05-03" version="1">MLNVVVVTDGPYGERAYENIKEKFDTEFVELEKPESMFMDEIDIPLYELNKIKEANILITYTQHPDLTLDIVDLVNKYVDHIIVAAWSGEGFKNQLESYENVTCPYIMCELEENGNEVFDSFTSKIGKPKVDITLENGKIAIIDVIRSSPCGSTKFVSDYLSEKYFGSDDLENVPIEAGLKLQHYPCRAAKMRLFTDEECKKEMASGLHRDAFEEALNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM2</accession>
    <name>F0TAM2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DEAD/H associated domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645445.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1873"/>
    <dbReference type="EnsemblBacteria" id="ADZ10094">
        <property type="protein sequence ID" value="ADZ10094"/>
        <property type="gene ID" value="Metbo_1873"/>
    </dbReference>
    <dbReference type="GeneID" id="10278331"/>
    <dbReference type="KEGG" id="mel:Metbo_1873"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OMA" id="VVDEWHE"/>
    <dbReference type="OrthoDB" id="995at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like_ATP-dep_RNA_helic_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="Lhr_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="41"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="266"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="863" mass="98824" checksum="C74D5609C6CD2421" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS0</accession>
    <name>F0TAS0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0695"/>
    <dbReference type="EnsemblBacteria" id="ADZ08946">
        <property type="protein sequence ID" value="ADZ08946"/>
        <property type="gene ID" value="Metbo_0695"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0695"/>
    <dbReference type="eggNOG" id="arCOG10263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190398_0_0_2"/>
    <dbReference type="OMA" id="NVEKCEY"/>
    <dbReference type="OrthoDB" id="126237at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="87" mass="9713" checksum="03E353EABCE32674" modified="2011-05-03" version="1">MIEKIEITTTSGDVHNFKKGEFGVESIEIDELRGLIEISYGSTEGGKKYVFIPLPSVEKCEFLDKEVSNPQDTSIKTKVTTAKTEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU2</accession>
    <name>F0TAU2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="2">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">coaD</name>
        <name evidence="4" type="ordered locus">Metbo_0717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644319.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0717"/>
    <dbReference type="EnsemblBacteria" id="ADZ08968">
        <property type="protein sequence ID" value="ADZ08968"/>
        <property type="gene ID" value="Metbo_0717"/>
    </dbReference>
    <dbReference type="GeneID" id="10277164"/>
    <dbReference type="KEGG" id="mel:Metbo_0717"/>
    <dbReference type="eggNOG" id="arCOG01223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035272_5_0_2"/>
    <dbReference type="OMA" id="FDTLHSG"/>
    <dbReference type="OrthoDB" id="93412at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="3">
        <location>
            <begin position="11"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="17192" checksum="D79CFDE7CCEE2587" modified="2011-05-03" version="1">MNEKSYKKVAVGGTFDKFHYGHMKLLDVAFEIGDHVLIGVTSNDFAGVKGRIDPCRVRMTNLRTLLKSKHQNYEIQELNDPYGTTVSNETIDAIVVSDETEPTAFKINEIRREKGMKALDIVTIHMVLAEDGKPISSTRIRKGEIDKKGTIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV1</accession>
    <name>F0TAV1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644328.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0726"/>
    <dbReference type="EnsemblBacteria" id="ADZ08977">
        <property type="protein sequence ID" value="ADZ08977"/>
        <property type="gene ID" value="Metbo_0726"/>
    </dbReference>
    <dbReference type="GeneID" id="10277173"/>
    <dbReference type="KEGG" id="mel:Metbo_0726"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_4_1_2"/>
    <dbReference type="OMA" id="FFCYCED"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="38461" checksum="D4C325D31F628514" modified="2011-05-03" version="1">MNSPRVAIVILNWNGWKDTIECLESVFQINYRNYTVVLVDNNSTDDSVQKIVEYAQGKINVESDFFDYSSLNKPIDYQILNKNGNVDKNPNKQLTLIINHKNDGFAEGNNIGMKYALENLESKYVLLLNNDTVVDKYFLDSLVKEGENNKKIGFLGSKIYYYDKPETIWCVGGKIDWKFARGLHIGNQEHDHGQYQIKNHFDYISGAALLIKKELLEDVGLLDEKYFLYFEETDLELRAALKGYENIYIPKSKIWHKVSRSGGGISNEVGLYYITRNRWIFMKKWAKPGDFLIFIILQIFMAITLPILLSIYHTNKNLFITYYRGLFDGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB52</accession>
    <name>F0TB52_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10198">
        <property type="protein sequence ID" value="ADZ10198"/>
        <property type="gene ID" value="Metbo_1978"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1978"/>
    <dbReference type="HOGENOM" id="CLU_3057128_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="5693" checksum="838A5761E26E0B58" modified="2011-05-03" version="1">MEDLSGLGKSASINSESAPKVCSRSSGISTQNALCDRTDCPSYGKWYCPFTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB71</accession>
    <name>F0TB71_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0771</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644373.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0771"/>
    <dbReference type="EnsemblBacteria" id="ADZ09022">
        <property type="protein sequence ID" value="ADZ09022"/>
        <property type="gene ID" value="Metbo_0771"/>
    </dbReference>
    <dbReference type="GeneID" id="10277219"/>
    <dbReference type="KEGG" id="mel:Metbo_0771"/>
    <dbReference type="eggNOG" id="ENOG502N58Z">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056490_0_0_2"/>
    <dbReference type="OrthoDB" id="142690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008417">
        <property type="term" value="F:fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006486">
        <property type="term" value="P:protein glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="PTHR11929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="363" mass="43446" checksum="28A128B571513054" modified="2011-05-03" version="1">MNVGFYNYYENCNKNRMFEDPSAHTVGDNLTYPFVHLFKTAQDAGINISTIDTNPLESYDKIFFFDFPKEDNIYFKQLVEVGHDNLYLFIYESEIVMPNNWDKSNYKYFKKVFTWNYEMVDDKKIFQYYFPIKVLKTNDFDVNKKNKLCAMVASNKFNYHPLELYSARLEALRWFERYHPEDFDLYGYGWEEQAKETLSTHWSTEEGIKRRNARPKEDPYISYRGSVDSKIETLKNYKFSICYENARDIPGYLTEKIFDSFFAGCIPIYWGEPNIKDFIPTETFIDKRDFDSYPELYDHIKNMSNEEYMNRISAIKEFVLSDGIYPYGEENFTRIVMNEIQSKEDQESSTSLVNQLIKIFKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBK4</accession>
    <name>F0TBK4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645624.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2054"/>
    <dbReference type="EnsemblBacteria" id="ADZ10273">
        <property type="protein sequence ID" value="ADZ10273"/>
        <property type="gene ID" value="Metbo_2054"/>
    </dbReference>
    <dbReference type="GeneID" id="10278516"/>
    <dbReference type="KEGG" id="mel:Metbo_2054"/>
    <dbReference type="eggNOG" id="arCOG10243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200161_0_0_2"/>
    <dbReference type="OrthoDB" id="130700at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="6954" checksum="2C2FE4E299F47A76" modified="2011-05-03" version="1">MSRILDYLKDWKNIATHSAVGIALLYFLIVAPLELWQRVAALGGVITFNIGRMHYDKYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBL3</accession>
    <name>F0TBL3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Abortive infection protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644441.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0840"/>
    <dbReference type="EnsemblBacteria" id="ADZ09090">
        <property type="protein sequence ID" value="ADZ09090"/>
        <property type="gene ID" value="Metbo_0840"/>
    </dbReference>
    <dbReference type="GeneID" id="24964520"/>
    <dbReference type="KEGG" id="mel:Metbo_0840"/>
    <dbReference type="eggNOG" id="arCOG02769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_629487_0_0_2"/>
    <dbReference type="OMA" id="TTFISMK"/>
    <dbReference type="OrthoDB" id="40501at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="444" mass="50372" checksum="1F530DCCDDADC8C8" modified="2011-05-03" version="1">MVQYDAQEYLNQGSKFHELGSLDKALTYYYKALDYLKGTDDKKTEADALLEIGNIYIEKENYENAQDYYEKSLNTYKTADDDIGEGYALTGIGLIHEKQEKYADARNYYVDAEAKFENRSDRERKAAIISLTAGTYEAQGAWEDAIMEYRRSLSIYRKVKDNDKQETIKDKIEHLSIERGKQKTSRSEKILAVSYVFALILAEVTVTYVNKETGLVIEALILMALLVNSSLNVSYNYSVLLRSMMALPMIRIIGLSIPLMQIQSLYWFPIIAVPLFAAAYTIMKNQGLTRQHIGLIWGNKKVQFLIALTGIFLGITEYIILQPKPLIAVLNPVNLIIASIILIISTGLAEELLFRGIIQKNAENVFGIFLGLLYTAVLFTALHIGWNNFYDLIFVFGVALFYGYAFQKTRSIVGVTLSHGISNSFLFLIVPFYAPLLFHYLPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP8</accession>
    <name>F0TBP8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644476.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09125">
        <property type="protein sequence ID" value="ADZ09125"/>
        <property type="gene ID" value="Metbo_0876"/>
    </dbReference>
    <dbReference type="GeneID" id="10277325"/>
    <dbReference type="KEGG" id="mel:Metbo_0876"/>
    <dbReference type="eggNOG" id="arCOG03950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207616_0_0_2"/>
    <dbReference type="OrthoDB" id="50074at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="276" mass="30819" checksum="4F89FCC4300DD956" modified="2011-05-03" version="1">MSKKIWVTVIALGLIAVIVGYSYVTVSNGPIEPLGRLSFVKIANPDMYPGHPHANLLGVYALQRNSKCAMVLHFAGSSNYRSFPELVNDTASHQTGNVYIIEVAFIDTQGGGSKSMSQIDLLDSLKVALYGVPDGRYKYMSDGTIYNTYDEMMAHVNQLAQQHNQTGPIPTVWHGTVRTDNPLMDPGCGFPLYFQIMTKTYGIVPAYVYTAYGLMQVFIYSPYWKYELAHATELQTLYNEGALNLDKSNVASNVDKYYQNLSKQETISQENLTNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX9</accession>
    <name>F0TBX9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Universal PUA-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2102"/>
    <dbReference type="EnsemblBacteria" id="ADZ10321">
        <property type="protein sequence ID" value="ADZ10321"/>
        <property type="gene ID" value="Metbo_2102"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2102"/>
    <dbReference type="eggNOG" id="arCOG00985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="OMA" id="GADIMKP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="PTHR22798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="63"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="15930" checksum="DE4325330FC99A54" modified="2011-05-03" version="1">MLRDMEGQLGDFSGIIKPKSKVEIIETDLDDIILVDGSPMIMMIDGEPFPTLKGALELDIKTKFVVVDMGAVKFVIKGADIMSPGITDADPNIVEGDLVVIVDETHHKPLATGRSLLTGPEMVENREGKAIKNIHHVGDEIWDLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC30</accession>
    <name>F0TC30_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644532.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0934"/>
    <dbReference type="EnsemblBacteria" id="ADZ09181">
        <property type="protein sequence ID" value="ADZ09181"/>
        <property type="gene ID" value="Metbo_0934"/>
    </dbReference>
    <dbReference type="GeneID" id="10277383"/>
    <dbReference type="KEGG" id="mel:Metbo_0934"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_0_0_2"/>
    <dbReference type="OMA" id="PQAYFEV"/>
    <dbReference type="OrthoDB" id="82596at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="193" mass="22140" checksum="CE6AC4A661769CE9" modified="2011-05-03" version="1">MKKTLENLTKAFIGESQARNRYTLYSKIAKNEGYVQIADIFLTTADNEREHAKWLFRMIQDLKKEFGDVEEISVEADAPLIMASTPENIISAIAGEHYENSEMYPEFADVAEEEGLDDMAVRLRAIGRAEEHHEARYKELLKVVEEGTVLKKDTKKEWLCSKCGYVHTGLTPPDKCPACDHPPKYYEILCEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC37</accession>
    <name>F0TC37_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644539.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0941"/>
    <dbReference type="EnsemblBacteria" id="ADZ09188">
        <property type="protein sequence ID" value="ADZ09188"/>
        <property type="gene ID" value="Metbo_0941"/>
    </dbReference>
    <dbReference type="GeneID" id="10277390"/>
    <dbReference type="KEGG" id="mel:Metbo_0941"/>
    <dbReference type="eggNOG" id="arCOG00362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_2_1_2"/>
    <dbReference type="OMA" id="RFEFMRE"/>
    <dbReference type="OrthoDB" id="80071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="157" mass="17538" checksum="1DEFAB06D5A624B1" modified="2011-05-03" version="1">MNRKKELKIVVLGSYNSGKTTTLENICQKKTKIEYKGTTIALDYGNVIVADEKIHIFGSPGQERFEFMREILAHKIDGAIVVIDSSKGLVESDEVIIEKLNLKDVPYVLFANKQDLNSCEIEHPQLRPDTPIIPTVAINGQGVEQGFLTLVELIKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC52</accession>
    <name>F0TC52_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644554.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0956"/>
    <dbReference type="EnsemblBacteria" id="ADZ09203">
        <property type="protein sequence ID" value="ADZ09203"/>
        <property type="gene ID" value="Metbo_0956"/>
    </dbReference>
    <dbReference type="GeneID" id="10277405"/>
    <dbReference type="KEGG" id="mel:Metbo_0956"/>
    <dbReference type="eggNOG" id="arCOG00519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068890_1_2_2"/>
    <dbReference type="OMA" id="AYYSRSG"/>
    <dbReference type="OrthoDB" id="63381at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="164" mass="18257" checksum="116B8B3A5AEEF987" modified="2011-05-03" version="1">MKTVIVFYSRTRKTAHVSKTLAKQINADTIEINDLKDRMGVINYLGSSLDALRENKTNIKPDTIDLSDYGLIYIGSPTWASKPAPAIITMIDKCDLKGKDVILFSTMGNSGGLKVVERMREKIEARGGRMVNSFTIKTSGKKIDELVEATEKILDEKDLKIYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC72</accession>
    <name>F0TC72_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0976"/>
    <dbReference type="EnsemblBacteria" id="ADZ09223">
        <property type="protein sequence ID" value="ADZ09223"/>
        <property type="gene ID" value="Metbo_0976"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0976"/>
    <dbReference type="eggNOG" id="arCOG01385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_19_0_2"/>
    <dbReference type="OMA" id="DDDCEAT"/>
    <dbReference type="OrthoDB" id="65802at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="329" mass="37501" checksum="8C43B76C1976AAE5" modified="2011-05-03" version="1">MIKITVGIIARNEEKNISETLKKVLNQSLDCSKYEVLVVDGNSDDKTREIAEKILKSSKISYKVLNEHDFGFYGHCFARNLVIDNSAETADYIAYTDADCLVDKKWLETLYRSILQSDDDVAGAGGPRLIAKTDNKTEMIINSLITSFIASGANPAFHSRNIKYVKSIANYNAIYKKSIISKFRYDKSLIMSDDNELNFRLRKAGYLFLNVPEAKIWHHETGSIKEFASNMFKYGVNISNTVKKHRSMITINVPLTTLFIAYIILLIPLYYFLGFITLIPMGLYILFAILVFFEVFMRTKSLYSVLVFILLPVEHVAYGLGVYSNLIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC87</accession>
    <name>F0TC87_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645705.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10354">
        <property type="protein sequence ID" value="ADZ10354"/>
        <property type="gene ID" value="Metbo_2135"/>
    </dbReference>
    <dbReference type="GeneID" id="10278598"/>
    <dbReference type="KEGG" id="mel:Metbo_2135"/>
    <dbReference type="eggNOG" id="arCOG06027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137928_0_0_2"/>
    <dbReference type="OMA" id="AHPEHWW"/>
    <dbReference type="OrthoDB" id="101098at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="13949" checksum="D78A8EEB4D57AE73" modified="2011-05-03" version="1">MIKIKRAYQPADEKDGFRILVDRLWPRGISKVKAKLDLWMKDIAPSNSLRQWFNHDPLRWDEFQSKYDEELKDKKEFLKQIKDLEKDKSIITLVYGAKDEEHNNAIVLRDVLKDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC90</accession>
    <name>F0TC90_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hydroxylamine reductase</fullName>
            <ecNumber evidence="6">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hybrid-cluster protein</fullName>
            <shortName evidence="6">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hcp</name>
        <name evidence="7" type="ordered locus">Metbo_2139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="6">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645708.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2139"/>
    <dbReference type="EnsemblBacteria" id="ADZ10357">
        <property type="protein sequence ID" value="ADZ10357"/>
        <property type="gene ID" value="Metbo_2139"/>
    </dbReference>
    <dbReference type="GeneID" id="10278602"/>
    <dbReference type="KEGG" id="mel:Metbo_2139"/>
    <dbReference type="eggNOG" id="arCOG02430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_0_0_2"/>
    <dbReference type="OMA" id="CAYAQGM"/>
    <dbReference type="OrthoDB" id="41554at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="6">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="434" mass="47761" checksum="5D3D5F5B00B16888" modified="2011-05-03" version="1">MPEKVDMFCYQCSQTAFETGCTVKGVCGKEATVARLQDNLLFSMKGISAYLYHARELGYTDPEVDAFIEKGFYSTLTNVNFDAGEFVKLATESGMMNIKAMQLLKKAHIETYGEPTPIEVKVGSVKGKGIIATGHSMKALEELLKQTEGTGINVYTHSELLPAHGYPGLRKYKHLVGQIGGPWFDQKKTFSKYPAAIVGTSNCVLIPKDEYKERMFTLGVAQIPGVKHIEDYDFSPVIETAKSLPDLEDEPRETVLTTGFGASTILSLAGKIKELVEAGKIKHFFVVGGCDSPKPQASYYTEFVKNLPEDTIVLTLACGKYRFNDMDLGDIEGVPRLIDIGQCNDAIVGIDVVVALSELFGMEINDLPLTFVLSWMEQKAAAILWSLLALNIQNMYLGPIIPGWVNEDILNVLVENYGLTPIGDPKEDIKKMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC95</accession>
    <name>F0TC95_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645713.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2145"/>
    <dbReference type="EnsemblBacteria" id="ADZ10362">
        <property type="protein sequence ID" value="ADZ10362"/>
        <property type="gene ID" value="Metbo_2145"/>
    </dbReference>
    <dbReference type="GeneID" id="10278608"/>
    <dbReference type="KEGG" id="mel:Metbo_2145"/>
    <dbReference type="eggNOG" id="arCOG02992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165492_0_0_2"/>
    <dbReference type="OMA" id="ITPVDVF"/>
    <dbReference type="OrthoDB" id="105481at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="112" mass="12596" checksum="D735650D356BB4EC" modified="2011-05-03" version="1">MKISKFADIECSPNPHGVDARKIYDTENAVVVHMTIKPGESLKKHSTPVDVFFYVLEGEGIVEIGDEKEKVSKDSLIESPAKTPHTWYNKGQNDLRILIVKVPRPHKSTKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD8</accession>
    <name>F0TCD8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645756.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2191"/>
    <dbReference type="EnsemblBacteria" id="ADZ10405">
        <property type="protein sequence ID" value="ADZ10405"/>
        <property type="gene ID" value="Metbo_2191"/>
    </dbReference>
    <dbReference type="GeneID" id="10278654"/>
    <dbReference type="KEGG" id="mel:Metbo_2191"/>
    <dbReference type="eggNOG" id="arCOG01042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131306_0_0_2"/>
    <dbReference type="OMA" id="RTFCYAT"/>
    <dbReference type="OrthoDB" id="104786at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="139" mass="16070" checksum="ACC38534B20DB8DC" modified="2011-05-03" version="1">MIHNISYRAFVYGTESKEKVLEAISTLFPNSSPQCEATEGYYKNQVLILHDKIDKKKPIKEFVELLSSMDDPAKKRIINQLEDKMDDYGNLFLRFDKQRAYQGDMKVVEHGDSIHTKIKIAAYPAKKKVALEIGRKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCJ8</accession>
    <name>F0TCJ8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644626.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1028"/>
    <dbReference type="EnsemblBacteria" id="ADZ09275">
        <property type="protein sequence ID" value="ADZ09275"/>
        <property type="gene ID" value="Metbo_1028"/>
    </dbReference>
    <dbReference type="GeneID" id="10277477"/>
    <dbReference type="KEGG" id="mel:Metbo_1028"/>
    <dbReference type="eggNOG" id="arCOG00143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="NLPVMAV"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="9"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="458" mass="48734" checksum="F5C1B3D8CB20479E" modified="2011-05-03" version="1">MKDSDRNKILALLFVGVFMGALDIGIIGPALPAIKAAFGINERLLSWIFTIYILFFMIGTPLMAKLSDNYGRKTIYILDVFLFAVGSAITVSSTSFETLLIGRAIQGFGAGGIFPVASAFIGDTFPQEKRGSALGLIGSVWGVSGLLGPILGGLLLNYGWQWLFIINIPIALVVILLGYKILPQSRKESKSSFDVKGTLVLTILVSSLAYGVNQIDTTNFLGSLSSVFTWPFIVIAVIMLPLLLKIEKNAEDPVLEVNLIKSREVKIATSIAFGTGLNQTAIVFLPAFAVTILALSISQASLMVIPLVVTLGLSAPIIGKLLDKYGTKRVMFVGTSILVVGLFMLSYLASSFYMFIISGAIIGIGLSSVLGSPLRYIMLSESPASKRASGQALININSSVGQLIGGALIGAVIASQGSSAQGYQSAYIVIGFVAIIMTLLTLGLKKREEQILTSKNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK7</accession>
    <name>F0TCK7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1037"/>
    <dbReference type="EnsemblBacteria" id="ADZ09284">
        <property type="protein sequence ID" value="ADZ09284"/>
        <property type="gene ID" value="Metbo_1037"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1037"/>
    <dbReference type="eggNOG" id="arCOG14000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700292_0_0_2"/>
    <dbReference type="OMA" id="HAVLNGY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17515" checksum="CF80982D9835999C" modified="2011-05-03" version="1" precursor="true">MNLKNSKNLLIMVIALFVIAASFITYFVHDYSIYTDKTFTNFIDSSNDTNVIESIDIQNSVLTDYVNSLYNSQVDSIESKYNSGTISLEEKNKQLNAVLKNRDMTVHTINKIDTAKKDVFEGKINQSDILIRINSFSSFNSDLKTEINETLNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCL1</accession>
    <name>F0TCL1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644639.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1041"/>
    <dbReference type="EnsemblBacteria" id="ADZ09288">
        <property type="protein sequence ID" value="ADZ09288"/>
        <property type="gene ID" value="Metbo_1041"/>
    </dbReference>
    <dbReference type="GeneID" id="24964528"/>
    <dbReference type="KEGG" id="mel:Metbo_1041"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="56"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="166" mass="19026" checksum="04CF15D4A3EE4199" modified="2011-05-03" version="1">MSEKNLKLKPNETATEESILDEESEVENELTTSERSINDILSTLKDKQEEFSQMISDYSIFNKRTLVDVIESTDSITIIADLPRLKNQDIEIAISEDSVDICAEMQEENTENVNYILRERSYGKIRRSIKLPSKVVAKDAEGTYKDAVLTIKLPKKQKNILRLKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM1</accession>
    <name>F0TCM1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644649.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1051"/>
    <dbReference type="EnsemblBacteria" id="ADZ09298">
        <property type="protein sequence ID" value="ADZ09298"/>
        <property type="gene ID" value="Metbo_1051"/>
    </dbReference>
    <dbReference type="GeneID" id="10277500"/>
    <dbReference type="KEGG" id="mel:Metbo_1051"/>
    <dbReference type="eggNOG" id="arCOG03349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141525_1_0_2"/>
    <dbReference type="OMA" id="MPEFANP"/>
    <dbReference type="OrthoDB" id="111254at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubrerythrin" evidence="1">
        <location>
            <begin position="20"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10916" checksum="F242E4BA8E3EAD94" modified="2011-05-03" version="1">MLSKIPIDIGKLKGEDLNREILRAAIIGELDAINLYEEFANMTDDQDIQIVLRDIAQEEKTHVGELQTLLLRVDEEQAIELEKGKKEVEELTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR3</accession>
    <name>F0TCR3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="6" type="ordered locus">Metbo_2239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645804.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2239"/>
    <dbReference type="EnsemblBacteria" id="ADZ10453">
        <property type="protein sequence ID" value="ADZ10453"/>
        <property type="gene ID" value="Metbo_2239"/>
    </dbReference>
    <dbReference type="GeneID" id="10278705"/>
    <dbReference type="KEGG" id="mel:Metbo_2239"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="OMA" id="DSGWLTY"/>
    <dbReference type="OrthoDB" id="75288at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00373">
        <property type="entry name" value="TIGR00373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="18995" checksum="B787BE8D5672CE02" modified="2011-05-03" version="1">MLKDPAVQEILMDITNDEQSSVSIIECLLNGKTTDEEIAEETEIRLNIVRKVLYKLYDAGIATYKRSKDPETQWYTYSWTFEQEKVADIVTEKFEKFSREIEESLEYEEGNMFFSCKLNGHRYKFDEASENNFECPECGGSLEYQDNSTIIVELKQMMDRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT6</accession>
    <name>F0TCT6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="1">PGP</shortName>
            <shortName evidence="1">PGPase</shortName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645827.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2262"/>
    <dbReference type="EnsemblBacteria" id="ADZ10476">
        <property type="protein sequence ID" value="ADZ10476"/>
        <property type="gene ID" value="Metbo_2262"/>
    </dbReference>
    <dbReference type="GeneID" id="10278728"/>
    <dbReference type="KEGG" id="mel:Metbo_2262"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="OMA" id="DTGYAYH"/>
    <dbReference type="OrthoDB" id="58398at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="23810" checksum="EA5EE12DC849133F" modified="2011-05-03" version="1">MIKVLATDVDGTITNTSRRGCLSAIETIHRVEDMGMPVIIVTGNISCFTNALSTFLSTSGGIVAENGGVIEFNGKTEILGNYKLCNEAYEYLKAELPVEKVQNSNLRVSEIALTRKVPVTTIKNVLKDHPVEVYDSKFAIHLTDPEVNKGSSLLKVAGALGFNAEEIMAVGDSENDMEFLAAAGLKIAVSNANEELKGIADYVTKNAHGDGFKEAVERFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU3</accession>
    <name>F0TCU3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="9">GGGP synthase</shortName>
            <shortName evidence="9">GGGPS</shortName>
            <ecNumber evidence="9">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_2269</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645834.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2269"/>
    <dbReference type="EnsemblBacteria" id="ADZ10483">
        <property type="protein sequence ID" value="ADZ10483"/>
        <property type="gene ID" value="Metbo_2269"/>
    </dbReference>
    <dbReference type="GeneID" id="10278735"/>
    <dbReference type="KEGG" id="mel:Metbo_2269"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="OMA" id="ADAIFFM"/>
    <dbReference type="OrthoDB" id="94629at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="169"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="200"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glycerol-1-phosphate binding" evidence="9">
        <location>
            <begin position="222"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="246" mass="25923" checksum="06ACB8924FF08906" modified="2011-05-03" version="1">MNVENYIRETLKDHKLHLTLLDPEEQTPEEAVSIAKNAVSGGTDGIMLGGSTTNSTELDATAKALKENVDVPIILFPGNITGVSKYADAIFFMSLLNSCNPYWITGAQALGAPTIKKLGIETISMGYLVVEPGGTVAWVGDAKVIPRKKPDLAAAYAMAADFMGMKLLYLEAGSGADEHIPKEMIQLVKKTTNSIVIVGGGIRTAQDAAEVAAAGADMVVTGTVVENSSNIKDKIQELVDGIRSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCU9</accession>
    <name>F0TCU9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645840.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2275"/>
    <dbReference type="EnsemblBacteria" id="ADZ10489">
        <property type="protein sequence ID" value="ADZ10489"/>
        <property type="gene ID" value="Metbo_2275"/>
    </dbReference>
    <dbReference type="GeneID" id="10278741"/>
    <dbReference type="KEGG" id="mel:Metbo_2275"/>
    <dbReference type="eggNOG" id="arCOG03197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140791_0_0_2"/>
    <dbReference type="OMA" id="DIRRGTH"/>
    <dbReference type="OrthoDB" id="81908at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="17893" checksum="C4C1B1CC0CB6A955" modified="2011-05-03" version="1">MSVRERRRIELNPDQVLFLTGPSGKLGLVKTGHDMQIFIGTPEKEEIVVFLGKDDLIAVSAFESGKKAEKGILAMMYLLKEFSSPIIVLPENHPTSQRLKMVVACGDHIKLDCDVQAGTHPEQDILCACSELSGLDIDAVEDGVEIEGNFEQFRMDKLDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV3</accession>
    <name>F0TCV3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-coenzyme M reductase operon protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645844.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2279"/>
    <dbReference type="EnsemblBacteria" id="ADZ10493">
        <property type="protein sequence ID" value="ADZ10493"/>
        <property type="gene ID" value="Metbo_2279"/>
    </dbReference>
    <dbReference type="GeneID" id="10278745"/>
    <dbReference type="KEGG" id="mel:Metbo_2279"/>
    <dbReference type="eggNOG" id="arCOG04859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="OMA" id="NFPYGYD"/>
    <dbReference type="OrthoDB" id="94905at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="17957" checksum="163B82650E0C937F" modified="2011-05-03" version="1">MDKIKAVDVKIFPYRRLKPQTTEKVLNGIMEFDGVLRVLVNGNSIPKVVNYGPAKGTPVNHEDRKVIGVKNENIELCVSVGELIITVNYDELESFMEKLDTLLKETLTMKYDVKTGIFTKTDITVSDYIKIGLGLENGIDPSLIGLVDPNSKSSETINLIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCW0</accession>
    <name>F0TCW0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645851.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2286"/>
    <dbReference type="EnsemblBacteria" id="ADZ10500">
        <property type="protein sequence ID" value="ADZ10500"/>
        <property type="gene ID" value="Metbo_2286"/>
    </dbReference>
    <dbReference type="GeneID" id="10278752"/>
    <dbReference type="KEGG" id="mel:Metbo_2286"/>
    <dbReference type="eggNOG" id="arCOG01791">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_2_0_2"/>
    <dbReference type="OMA" id="ITCMFGS"/>
    <dbReference type="OrthoDB" id="49237at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="46"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="252" mass="29033" checksum="549DCBD5779B3BE0" modified="2011-05-03" version="1">MTEEQLYKNFARYYDKIYKGMDYEGEVEFIKWAVHKHQPSSTNLLMDVACGTGTHAQKLTDNFKITGVDLSSQMLEIAREKVPNVNFIQGDMKNLNIGSKFDVITCIFSAIHYNRNLKELGDTIQNFYEHLNPRGILIFDLSLNKNNWIEGLVSVDTVVEEDLKIARICQSKLKDGIFNANFVFLVKDDGEFDFDIDQHELGVFEVEEVINLMENKKFETFVYAGFKSEEWTENSVERPVFVGVKNLRGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5X1</accession>
    <name>F0T5X1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09364">
        <property type="protein sequence ID" value="ADZ09364"/>
        <property type="gene ID" value="Metbo_1119"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1119"/>
    <dbReference type="HOGENOM" id="CLU_3178574_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="46" mass="5388" checksum="15ED9E0AD5F13B1A" modified="2011-05-03" version="1">MKKCRIFCDVYGANVNHSECMKCKLKKDCIKVKLPILNNKDLIKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y7</accession>
    <name>F0T5Y7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644731.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1136"/>
    <dbReference type="EnsemblBacteria" id="ADZ09380">
        <property type="protein sequence ID" value="ADZ09380"/>
        <property type="gene ID" value="Metbo_1136"/>
    </dbReference>
    <dbReference type="GeneID" id="10277586"/>
    <dbReference type="KEGG" id="mel:Metbo_1136"/>
    <dbReference type="eggNOG" id="arCOG07809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_384807_0_0_2"/>
    <dbReference type="OrthoDB" id="13682at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="711" mass="82316" checksum="E01D787E08A38EFA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T602</accession>
    <name>F0T602_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hisC</name>
        <name evidence="5" type="ordered locus">Metbo_2295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645860.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2295"/>
    <dbReference type="EnsemblBacteria" id="ADZ10509">
        <property type="protein sequence ID" value="ADZ10509"/>
        <property type="gene ID" value="Metbo_2295"/>
    </dbReference>
    <dbReference type="GeneID" id="10278761"/>
    <dbReference type="KEGG" id="mel:Metbo_2295"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="OMA" id="IWLNANE"/>
    <dbReference type="OrthoDB" id="69863at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3 5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="33"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="366" mass="41080" checksum="D1E8F494A105420E" modified="2011-05-03" version="1">MIKINKVVKELDPYVPGRSVDDIAKTYGLEPENIIKLGSNENPMGPSPKAVEALKKNLKLIHQYPESNLETLKGEIAEYSQVSPSNVIIGGDGADEIIDVLGKTFTEPGDEFIVPIPGYMYYEFMLKIQGAVPVYAKWDVPSNTLDVNSVLDTITPKTKLIFLCTPNNPTGGLIPRPDLIKIIENTDALVVVDEAYIEFSDHRNVDLLEKYENVFILRTFSKVLGLAGMRIGYGLSNPTVIEYMHRVKPVFSLTKLSEVAASATLNDEEYIKKSTELSLKSRELLYTEMSKFNELTVYESASNYILVDVRKTGFTAKELTQKLMEKGVIVRDCTSFKGIDEYWIRVSVGTMEEDERFIKILGKLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T605</accession>
    <name>F0T605_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645863.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2298"/>
    <dbReference type="EnsemblBacteria" id="ADZ10512">
        <property type="protein sequence ID" value="ADZ10512"/>
        <property type="gene ID" value="Metbo_2298"/>
    </dbReference>
    <dbReference type="GeneID" id="10278764"/>
    <dbReference type="KEGG" id="mel:Metbo_2298"/>
    <dbReference type="eggNOG" id="arCOG09465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286358_0_0_2"/>
    <dbReference type="OMA" id="YEASCIS"/>
    <dbReference type="OrthoDB" id="137239at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="22992" checksum="F53419ABAD1E993A" modified="2011-05-03" version="1">MRDRLQDFINWFFDQSLRNKIGIIIAAAVVIILIALLVFNLVYGDAPLTGNALSQYEASCISVGYQELSTNIGKYTGQHVKFSGKVVSINTNNGKTQIVMSVTPVNGGWSTSDLIFVSYNAQTQFKAGDIVTVYGDVVGTYNYVSISNGQLIIPKITARYMEATPIAVPTVVSVPFTSPSNNQTNSSTPDNSANITPINSTPAPTSSNTNNLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T613</accession>
    <name>F0T613_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10520">
        <property type="protein sequence ID" value="ADZ10520"/>
        <property type="gene ID" value="Metbo_2306"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2306"/>
    <dbReference type="HOGENOM" id="CLU_2340201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12248" checksum="F6B3BA62E0EEFABE" modified="2011-05-03" version="1">MKSYISIIIIIIGVIAFLVTRIVHDPSYNFSLGAIIIIIIGLIWMYFDSKGSDRKFNFRKTDSVDNCTKCKYYNKRSFNGVNADCKFLYIKVDEFHVCDLYKKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T617</accession>
    <name>F0T617_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10524">
        <property type="protein sequence ID" value="ADZ10524"/>
        <property type="gene ID" value="Metbo_2310"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2310"/>
    <dbReference type="HOGENOM" id="CLU_887431_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="34247" checksum="1EF6B38EC955978A" modified="2011-05-03" version="1" precursor="true">MIKNVFLMLLMLALITSIGGIEGVSATNPCETHIGTNESFSYAHNVYMRNQADGQYVIYQKPKGYNNTKWIKYLFYRVQDDSRLLDTYKLVEIQNVTLVNNNLYSVNFPRTSVASVMAGTTSEEMGRGFESHVILISDDGSDIGVNSGTILSSINFYHESNSFTPNGDLSGLAKLQIIIKNNLYQGSMIAGNSTTTYTWNGETCTIATVDKWTKVLSDTEIASGKLAVLHGAASTMSPDDNGGVFNRDPSNKIYAHPGVNSTIIGESGGNVIGYGWFSDVTTEGWTLALYEHYDVAVNTVMSFLYTITAYVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T618</accession>
    <name>F0T618_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10525">
        <property type="protein sequence ID" value="ADZ10525"/>
        <property type="gene ID" value="Metbo_2311"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2311"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949310_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="6266" checksum="AFBB2AB4D93D2DB8" modified="2011-05-03" version="1" precursor="true">MGLKKKSIFFVMIIFAFLILFTGSVSATNDTTAPTVTDINPDTGATNVPGNIIINVTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T649</accession>
    <name>F0T649_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645907.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2343"/>
    <dbReference type="EnsemblBacteria" id="ADZ10556">
        <property type="protein sequence ID" value="ADZ10556"/>
        <property type="gene ID" value="Metbo_2343"/>
    </dbReference>
    <dbReference type="GeneID" id="10278809"/>
    <dbReference type="KEGG" id="mel:Metbo_2343"/>
    <dbReference type="eggNOG" id="arCOG02822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047362_0_0_2"/>
    <dbReference type="OMA" id="VIAPVHC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="100"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="447" mass="49520" checksum="D07368EFA9E0F1F9" modified="2011-05-03" version="1">MDYLVVDMTHGGLIIASELSKLPETHVYAWDIYRTLDESIIEKYSSSSLEFVDGNCFKEPKELTVVSPIHCNLQRHVDMTHHDAVKFILNHKINVPVVEVTGVKGKTSVVWILKEILKHKKPLVLSSLGVEVFDGTKWVKLKGNISITPASIVEAWKLAENYEIGICIFETSLGGTGLADVGVLTNIAENYSIAGNSSNASKAKSQIFKSKMVACDQESFDSKYIKYKENTNTFGILNNSKIIPDLTATNIKYSFDKTDFDVQLSHFKTISSGLIEGTFKLNTFAPAPIHVNNVLAAVSASVMLGISEEKIKTGLLQFKGLNGRTTIKELNGVRIVEEINPGLNVTAVKEAIKMMENIDDVAVVFGGRYGVTCEEIDENSVSQVLNGINEDTKLILVDELGKNVNKLLKRNSIYYNNLKDAVNYASKTNSHNILVVYRSNYSDLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6M1</accession>
    <name>F0T6M1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenylosuccinate lyase</fullName>
            <shortName evidence="9">ASL</shortName>
            <ecNumber evidence="5 9">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643605.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0001"/>
    <dbReference type="EnsemblBacteria" id="ADZ08254">
        <property type="protein sequence ID" value="ADZ08254"/>
        <property type="gene ID" value="Metbo_0001"/>
    </dbReference>
    <dbReference type="GeneID" id="10278944"/>
    <dbReference type="KEGG" id="mel:Metbo_0001"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="39322at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="10">
        <location>
            <begin position="362"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="50231" checksum="3A07C3C7247F026C" modified="2011-05-03" version="1">MAIHPIEFRYGTEEMREIWETDNKLQKMLEVEAALAEAEADLDIIPKNAALEIKSKASTKFVTSKRVSEIEAETNHDIASIVKALAEVCNDGAGEYVHFGATSNDIIDTSQALLFKETLKVLKTRLTELTKIVLRLADENKTNVCIGRTHGQHALPTTYGMKFAIWADELHRQLNRLDECEERLCVGMVTGAVGTTAALGKEGLDIHKKVSKILDLKPVMISSQVIQRDAHAEFIMDLANIASTLEKMALEVRNLQRTEINELGESFDPSKQVGSSTMPHKMNPITAERICGISRVVRAYVAPALQNNPLWHERDLTNSSCERIILPESCMLTDYMIKLSIRLFSNLVFKPENIEKNLNLSNGLIMAERVMAELTRKGMGKQTAYSLVRKCSLEAYDLDTGLKQVIESNDEIKAYLSSSDIELIMDPHTYIGSSVEMVNEVLVASKNWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N7</accession>
    <name>F0T6N7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="7">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">leuS</name>
        <name evidence="11" type="ordered locus">Metbo_0017</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643621.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0017"/>
    <dbReference type="EnsemblBacteria" id="ADZ08270">
        <property type="protein sequence ID" value="ADZ08270"/>
        <property type="gene ID" value="Metbo_0017"/>
    </dbReference>
    <dbReference type="GeneID" id="10276439"/>
    <dbReference type="KEGG" id="mel:Metbo_0017"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="OMA" id="AWNMAFQ"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="PTHR45794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="9">
        <location>
            <begin position="9"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="10">
        <location>
            <begin position="708"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="7">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="7">
        <location>
            <begin position="622"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="958" mass="111258" checksum="6299A3CD0D6B95CD" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6R8</accession>
    <name>F0T6R8_METLA</name>
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        <submittedName>
            <fullName evidence="6">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0048</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643652.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0048"/>
    <dbReference type="EnsemblBacteria" id="ADZ08301">
        <property type="protein sequence ID" value="ADZ08301"/>
        <property type="gene ID" value="Metbo_0048"/>
    </dbReference>
    <dbReference type="GeneID" id="10276471"/>
    <dbReference type="KEGG" id="mel:Metbo_0048"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OMA" id="FLVEMFP"/>
    <dbReference type="OrthoDB" id="38526at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
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        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
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        <location>
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            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="5">
        <location>
            <begin position="17"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="419" mass="46481" checksum="AACF624BC7F19664" modified="2011-05-03" version="1">MSLLNQHKKLNSIHYRIFGISWAGWVFDFYDLILFTFLIIPIGHELNLSTIMLSYALSASILAAAVGGVIFGVLADKYGRKPVLQWTIVIYSIGTFLCAFSTNLETLILFRIITGLGVGGEWATGQTYVNETFPAHLRGKFGSLLQTGNPVGFILAALVGGFLAPIIGWREAFFVSVIPAILVIFIRRKIPESDVWIMNNVSSGKNRVKKTLLNKKNQFFELFTKKYRKLFFKALLLAILGSSAYWFTYSWLPTYLLQQNISISKSALWIIVNQVGGIIGLLIFGFIADKFGRRPAFSIFATTMAFGLIMITIFWSNIQAYPPFIFIFMFIIGIGTGIYGGYGPIVSELFPTEIRSTALGAGFNLARGTQFLTPIILAGIAIYYGLGSGIFLGSIFALSVAIFIWTFPETKGIELESLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S3</accession>
    <name>F0T6S3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein (LYSE/YGGA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643657.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0053"/>
    <dbReference type="EnsemblBacteria" id="ADZ08306">
        <property type="protein sequence ID" value="ADZ08306"/>
        <property type="gene ID" value="Metbo_0053"/>
    </dbReference>
    <dbReference type="GeneID" id="10276476"/>
    <dbReference type="KEGG" id="mel:Metbo_0053"/>
    <dbReference type="eggNOG" id="arCOG01947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104651_0_0_2"/>
    <dbReference type="OMA" id="NPYFWIW"/>
    <dbReference type="OrthoDB" id="92826at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="215" mass="23060" checksum="191F9FD13A26F8CE" modified="2011-05-03" version="1">MLDNVWIGIILFTATSFIVGLSGAMVPGPMLTVTISDSLKKGATAGPKIVFGHIITEFILILLIFAGLGWLIGSETAIFVIGAIGGLIMVLMGFQMARSSNSLQDLQKSSETSKGYGPIINGILTSVSNPYFFIWWATVGWAFMLKGIELAGILGVTGFLVGHWCSDLGWFGTVSIFTTKGSNIMKDNHYKIIMSVSGIFLMILGVYFVISSCVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6W3</accession>
    <name>F0T6W3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1241"/>
    <dbReference type="EnsemblBacteria" id="ADZ09483">
        <property type="protein sequence ID" value="ADZ09483"/>
        <property type="gene ID" value="Metbo_1241"/>
    </dbReference>
    <dbReference type="GeneID" id="10277692"/>
    <dbReference type="KEGG" id="mel:Metbo_1241"/>
    <dbReference type="eggNOG" id="arCOG10865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Big_5" evidence="1">
        <location>
            <begin position="10"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="31518" checksum="716A1A9F677010F4" modified="2011-05-03" version="1">MGSSSASAVKNPYVISVDPSHGSIDIPANKAINVIFNENVTLNNGLVELKSSSNQSVPISLTLGKQKLRITPNCLLKNGEKYMLYIHTGSFKGLKNNYNKLFISSFRVSLTPPTSSKVYFQVLSPEKTVNGTKTYSKYAVYQKPAYSAYWNRYLFYHMVNPAVNGTNGPEDVYRIYKLQNIVSNSSYISPKPIQSTDLTTPGNWEKAVQIAGNPLMGGMHGYEMVNSIFYYGDGIKFIPTMNLMNEFNKLEIVETSDLLNPKNNSQKVAKTVTKYIWNGASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X8</accession>
    <name>F0T6X8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644849.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1256"/>
    <dbReference type="EnsemblBacteria" id="ADZ09498">
        <property type="protein sequence ID" value="ADZ09498"/>
        <property type="gene ID" value="Metbo_1256"/>
    </dbReference>
    <dbReference type="GeneID" id="10277707"/>
    <dbReference type="KEGG" id="mel:Metbo_1256"/>
    <dbReference type="eggNOG" id="arCOG09581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111928_0_0_2"/>
    <dbReference type="OMA" id="AKFFFSI"/>
    <dbReference type="OrthoDB" id="109402at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012963">
        <property type="entry name" value="DUF1700"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08006">
        <property type="entry name" value="DUF1700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="22093" checksum="E2C78EBAB31A9777" modified="2011-05-03" version="1">MNKEEYLKKLSKQIKKMSVDEKRDLLQDYEEHFRIGMEKGRREEEISKALGDPKNVAKQIKADYMVKKAEDKPSPTSIIEALAAVAGLGLFNIFVAVPSLLLAVVIISLVLAGSAVIVIGILITLSPLIHVFLPNFIYLPEYKGVGTLLWVLFGIALTVVGTIFVLVMSYVANKLYQMVIKLLKSNLEDIKENTLVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z6</accession>
    <name>F0T6Z6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1274"/>
    <dbReference type="EnsemblBacteria" id="ADZ09516">
        <property type="protein sequence ID" value="ADZ09516"/>
        <property type="gene ID" value="Metbo_1274"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1274"/>
    <dbReference type="eggNOG" id="arCOG01500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050481_0_0_2"/>
    <dbReference type="OMA" id="CARCEEC"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12798">
        <property type="entry name" value="Fer4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="460" mass="50662" checksum="52E3C9DCF601E13A" modified="2011-05-03" version="1">MITTTTNNSKKYCKPIRDVEIDYNIDSSKCEACEDKPCLMSCPVDAIWKTEDGKIEIDDKCVGCVLCREACPYNAINMKTTLSEPIRENVPNINTKLCRQCGACVKACKTGSIQLISSGNEEAHSEINEDTCVRCGYCARVCPTEAIKYGEILPRSVVGGKAIVVNQKDCIGCMTCTKVCPSRGAINVGKVSKLPFINPSYCARCEECMNVCPSAAIKYSSRKRAYKNFSKIKTMEIVSELLENEAGKLSKDAIRISSILNGIARKISSNHSEKSFKEDVTPIITEEIKAMTHNELEIEDINEIIGVTNPKRSIKVIEDNCIGCGACMSECPVKCIELEMPSPVHIDDRCVHCGKCIETCQFNAIELAEEYFKVEDGKIFFKRDALTGQREGKIVTDVEACMACGICVRKCPTNALKLEKDEVIVDTEKCILCGECDIICPVNAIKLKTESNTASTEANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T726</accession>
    <name>F0T726_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="6">Glu-ADT subunit E</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatE</name>
        <name evidence="8" type="ordered locus">Metbo_2447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646011.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2447"/>
    <dbReference type="EnsemblBacteria" id="ADZ10660">
        <property type="protein sequence ID" value="ADZ10660"/>
        <property type="gene ID" value="Metbo_2447"/>
    </dbReference>
    <dbReference type="GeneID" id="10278919"/>
    <dbReference type="KEGG" id="mel:Metbo_2447"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="OMA" id="SGFQRTM"/>
    <dbReference type="OrthoDB" id="35861at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
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        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="PTHR11659:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="7">
        <location>
            <begin position="479"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="626" mass="70449" checksum="FE793119A95D3AF0" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T743</accession>
    <name>F0T743_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin:thioredoxin reductase</fullName>
            <ecNumber evidence="5">1.8.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ferredoxin-thioredoxin reductase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Metbo_2464</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalytic subunit of the ferredoxin-thioredoxin reductase (FTR), which catalyzes the two-electron reduction of thioredoxins by the electrons provided by reduced ferredoxin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [thioredoxin]-dithiol + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:42336"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer of subunit A (variable subunit) and subunit B (catalytic subunit). Heterodimeric FTR forms a complex with ferredoxin and thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ferredoxin thioredoxin reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.8.7.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646028.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2464"/>
    <dbReference type="EnsemblBacteria" id="ADZ10677">
        <property type="protein sequence ID" value="ADZ10677"/>
        <property type="gene ID" value="Metbo_2464"/>
    </dbReference>
    <dbReference type="GeneID" id="10278936"/>
    <dbReference type="KEGG" id="mel:Metbo_2464"/>
    <dbReference type="eggNOG" id="arCOG01100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536690_0_0_2"/>
    <dbReference type="OMA" id="CDYRDPD"/>
    <dbReference type="OrthoDB" id="82333at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016730">
        <property type="term" value="F:oxidoreductase activity, acting on iron-sulfur proteins as donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004209">
        <property type="entry name" value="FTR_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036644">
        <property type="entry name" value="FTR_bsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35113">
        <property type="entry name" value="PTHR35113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02943">
        <property type="entry name" value="FeThRed_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57662">
        <property type="entry name" value="SSF57662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="15">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ10677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="169" mass="19246" checksum="E4A14A17696C2555" modified="2011-05-03" version="1">MSDFDEPDEQEVDSYYQKVKADAESGGYHLNSDEEFTKELLKSILINKKRYGYGACPCRLASGTKEEDLDLICPCDYRDPDLFDYDACYCGLYVSGKILSGESELQSIPERRPTAEERKKIQASKSETVKNLSKPVWRCNVCGYLCARDAPPETCPICKVSSDRFERFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T792</accession>
    <name>F0T792_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="36351at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043256">
        <property type="entry name" value="MvhB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005658">
        <property type="entry name" value="FwdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="227"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="347" mass="37667" checksum="440E8D45AFCED42B" modified="2011-05-03" version="1">MAANIKEVKAKDFNVERSAEEDRELSFKDESCVGCGVCESICPVGAIKLGSVGAIVRNDIDESKIEIDEEKCVLCGMCSVGCPVDALEFTVDGTPISEIETYPKLISSAEIDDETCIYCKACERACPREAITVARQLPDRSKLVTGEIDIDKETCIDCGICEEMCPADAITIDQKGPEDFDIAVDKDKCVYCLVCKKACPVDAIMAACRTCSYGEYDLNPEDAETTGKALIDDDACVRCGWCEEVCPVDAAKVEKPFEGELSLDLDKCTTCGACVDVCPCDVLFFPKSSAPGTIDDKLMKDEQFCIYCGACENVCPVEAIDVKRTGIKCTPTKSKSWQKSIDSLKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H7</accession>
    <name>F0T7H7_METLA</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644896.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1303"/>
    <dbReference type="EnsemblBacteria" id="ADZ09545">
        <property type="protein sequence ID" value="ADZ09545"/>
        <property type="gene ID" value="Metbo_1303"/>
    </dbReference>
    <dbReference type="GeneID" id="10277754"/>
    <dbReference type="KEGG" id="mel:Metbo_1303"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_0_5_2"/>
    <dbReference type="OMA" id="SWWHGRY"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
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        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="277" mass="31501" checksum="051D4CE64E626999" modified="2011-05-03" version="1">MLTLSAIILNYKDPELTARSVQALLESAQAINVKAQVVVVDNSATETAESLKKLLGDDITLIENRENQGFSRANNQGMEVATGDYILLLNNDAFVNSETLHMGLNYLDNHEKCGVWSPKLVGLDDKFQVSCARLPSLRGLIAEYIQFKNLDWYPDLEKWNEPTDVGNVVGAFMLFRRKLLDEVGMLDEDYFFNVEDVDYCKRIHEAGYEVIYDPRYSVVHIGGASQPGKWVNDPYMHKNRKIYFKKNHGAVQGIIAGLVIWTGLNMRRFMMWRAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J3</accession>
    <name>F0T7J3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644912.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1319"/>
    <dbReference type="EnsemblBacteria" id="ADZ09561">
        <property type="protein sequence ID" value="ADZ09561"/>
        <property type="gene ID" value="Metbo_1319"/>
    </dbReference>
    <dbReference type="GeneID" id="10277770"/>
    <dbReference type="KEGG" id="mel:Metbo_1319"/>
    <dbReference type="eggNOG" id="arCOG02717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149108_3_0_2"/>
    <dbReference type="OMA" id="PEITTNM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00304">
        <property type="entry name" value="TIGR00304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="94" mass="10187" checksum="F7D35F25973BFBFE" modified="2011-05-03" version="1" precursor="true">MVNSNLLILAGFGVVMLGIIMIFLGSLLHSQSNNQSNSSDSDSSKFNIQTGGVIMIGPIPIIFGNNKGMVGVSVVFAIILMIIYFLLFYRGRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L3</accession>
    <name>F0T7L3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644932.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09581">
        <property type="protein sequence ID" value="ADZ09581"/>
        <property type="gene ID" value="Metbo_1340"/>
    </dbReference>
    <dbReference type="GeneID" id="10277791"/>
    <dbReference type="KEGG" id="mel:Metbo_1340"/>
    <dbReference type="eggNOG" id="arCOG02572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_4_2_2"/>
    <dbReference type="OMA" id="FADETHY"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="20696" checksum="FF472B86E799FBD7" modified="2011-05-03" version="1">MKIIGLTGSPREGSNTEVLVNEILKGASEAGAETKSYNLVKMDIKPCIACMHCKTHGSQCATEDDMQEFYKELRESDGFVVGSPLYFGQMSAQAKLLIDRLFAFYNPEAGDMERKKVAFTFSQGNPDEATFKDYYDYIRNVFEMAYDLVDVLVSKGNQLPGDVKNNEHNLAKAKKIGKEMASKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P0</accession>
    <name>F0T7P0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1368</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644959.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1368"/>
    <dbReference type="EnsemblBacteria" id="ADZ09608">
        <property type="protein sequence ID" value="ADZ09608"/>
        <property type="gene ID" value="Metbo_1368"/>
    </dbReference>
    <dbReference type="GeneID" id="10277819"/>
    <dbReference type="KEGG" id="mel:Metbo_1368"/>
    <dbReference type="eggNOG" id="arCOG02254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167318_1_0_2"/>
    <dbReference type="OMA" id="RIGKRMK"/>
    <dbReference type="OrthoDB" id="121332at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="109" mass="12608" checksum="57EAC2276B62421D" modified="2011-05-03" version="1">MYKDELIQIHQFLVYVLKNLENEYTVKDECKDYICLNISPHHIHRTKAEHKYAIFVLSTAISEIIANNNGGTSTNISNGLGELVKRSKKELIKFQDDGALRYQNQKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S0</accession>
    <name>F0T7S0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">S-adenosylmethionine synthase</fullName>
            <shortName evidence="14">AdoMet synthase</shortName>
            <ecNumber evidence="6 14">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mat</name>
        <name evidence="15" type="ordered locus">Metbo_0155</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AL-21</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="6 14"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643758.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0155"/>
    <dbReference type="EnsemblBacteria" id="ADZ08407">
        <property type="protein sequence ID" value="ADZ08407"/>
        <property type="gene ID" value="Metbo_0155"/>
    </dbReference>
    <dbReference type="GeneID" id="10276581"/>
    <dbReference type="KEGG" id="mel:Metbo_0155"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="OMA" id="IGHPDSI"/>
    <dbReference type="OrthoDB" id="21658at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="PTHR36697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 15" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="135"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADZ08407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="401" mass="44041" checksum="13978B16896ADCB7" modified="2011-05-03" version="1">MRNIIIKELIQKPIEEQEVEIVERKGIGHPDSISDGIAESVSRALCHAYLENFDGILHHNTDEVQITAGESDPHFGGGEIIKPIDILLTGRGVAEFEGKKIGIDRIAIMAAKNYLKENIINLDVESSAVVECKIGHGSGDLVDVFGRDGMPSSNDTSFGVGFAPFSETEKIVMEVEELLNSKDFKKKYPQVGEDIKVMGLRENNKITLTTAVAMVSKYVDGRDTYINVKEELNDIITDLAQNKTEREVETFINTGDDDNSKTEKGYYLTVTGTSAEMGDDGSVGRGNRANGLITPNRPMSMEATSGKNPINHVGKIYNLLSNKMADQITSEIDGIQQIHIMILSQIGKPIDHPKAASAQIIVEDGYSISSVHKDVQGVMDNWLEDIGKITEMMVKGKIRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T0</accession>
    <name>F0T7T0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Auxin Efflux Carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643768.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0165"/>
    <dbReference type="EnsemblBacteria" id="ADZ08417">
        <property type="protein sequence ID" value="ADZ08417"/>
        <property type="gene ID" value="Metbo_0165"/>
    </dbReference>
    <dbReference type="GeneID" id="10276591"/>
    <dbReference type="KEGG" id="mel:Metbo_0165"/>
    <dbReference type="eggNOG" id="arCOG04756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056175_5_2_2"/>
    <dbReference type="OMA" id="LAMWITY"/>
    <dbReference type="OrthoDB" id="66562at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="300" mass="32187" checksum="88D522DB9D0E3816" modified="2011-05-03" version="1">MSSTETILAIIVMILIGYFSKKIGLLKPEDSTTLNKIVVNIAIPSLIFLAMYGADLSNINSLFPITIICLITGTLSGTVVYLFARYRGYSKKTRWTIVGTSTLFNSGFLGYPVVLGVYGSTGLVRAVFFDMGSTILFLCLGILFIVLFGGKYSSIIKRTVLFPPLWGIILGIVVNLLHLNIGPLPLDVLKYLSGAAIPIIMISLGLSLEVGGLKNYLDAATFVSTVRLIISPAIAFVIVTILGFSYINSTVTVVEAGMPSAMLSLVLAASYDLDVKAAAACIFMSTVLSMITLPILIYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T1</accession>
    <name>F0T7T1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA</name>
        <name evidence="9" type="ordered locus">Metbo_0166</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643769.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0166"/>
    <dbReference type="EnsemblBacteria" id="ADZ08418">
        <property type="protein sequence ID" value="ADZ08418"/>
        <property type="gene ID" value="Metbo_0166"/>
    </dbReference>
    <dbReference type="GeneID" id="10276592"/>
    <dbReference type="KEGG" id="mel:Metbo_0166"/>
    <dbReference type="eggNOG" id="arCOG00415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_0_0_2"/>
    <dbReference type="OMA" id="TFRIYLR"/>
    <dbReference type="OrthoDB" id="30130at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01123">
        <property type="entry name" value="Rad51_DMC1_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033925">
        <property type="entry name" value="Rad51_DMC1_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="75"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="8">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="311" mass="33863" checksum="5CEBAD186267B165" modified="2011-05-03" version="1">MVELEELPNVGEKTAQKLRDAGFADMMRLATATAKELSVKAEIGEGVAEKVIEAARKSEQIDFETAMDVMERRKDVGRVTTGSTGLDELIGGGIETQSITEVFGEFGSGKSQISHELAVTVQLPPEKGGLGGQCVFIDTENTFRPERIKQIAEGFELDVEEVLTNIHIARAFNSSHQILMADKVNELIQSGANIKLVIVDSLTAHFRAEYVGRESLATRQQKLNQHLHTLSNIANTYNVAVFVTNQVQSKPDAFFGSPTKAIGGHVLGHAATYRIWLKKGLAGKRIARLVDSPHLPEGEAVFKVITEGISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7T3</accession>
    <name>F0T7T3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643771.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0168"/>
    <dbReference type="EnsemblBacteria" id="ADZ08420">
        <property type="protein sequence ID" value="ADZ08420"/>
        <property type="gene ID" value="Metbo_0168"/>
    </dbReference>
    <dbReference type="GeneID" id="10276594"/>
    <dbReference type="KEGG" id="mel:Metbo_0168"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_11_3_2"/>
    <dbReference type="OMA" id="QFQYIDA"/>
    <dbReference type="OrthoDB" id="94646at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="140" mass="16531" checksum="D80E3818F346C704" modified="2011-05-03" version="1">MNQELEIVESMDKLANIMQKFQTELLTGELKKYTLRQLYYIELIDKNENISISEISRMLDLKKSTVSIAVNQLIEQNIVLKIQSDADKRFYFLKLTDKGDKIMQMHKQVHRNTIKKILTILDQNEVEKFIEIVNKINQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7V1</accession>
    <name>F0T7V1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MgtE intracellular region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643789.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0186"/>
    <dbReference type="EnsemblBacteria" id="ADZ08438">
        <property type="protein sequence ID" value="ADZ08438"/>
        <property type="gene ID" value="Metbo_0186"/>
    </dbReference>
    <dbReference type="GeneID" id="10276612"/>
    <dbReference type="KEGG" id="mel:Metbo_0186"/>
    <dbReference type="eggNOG" id="arCOG00634">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030870_1_0_2"/>
    <dbReference type="OMA" id="PARRDWE"/>
    <dbReference type="OrthoDB" id="55490at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="284"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="348"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="413" mass="46409" checksum="9E7DA98F63EFF114" modified="2011-05-03" version="1">MYLSDFIKKPVLSPSGEQIGNLKDVVVSSDHSYPILKALEITVTGKQIKNISWRCVEKIGKEVKLNCSLEEIKDYEIQKHDVLLLKDVMDRQVVDIEGKKIRRVNDIKISPTNGHYHVIGVDIGVNGILRRLGLNRIVKSLGITSTEDLISWRDIDPVESDYSKVKLKVPKQKIKKLHPADMAEIVDQLGLNESLNILNSLDDESAADTLEEVSPERQVSILEGMDSQRAAEILDEMSPDDAADVLADLPEEKAEELLDLMEPEESNDLRKLLRYPENTAGGIMTTEFAYVNQGLTVKEVLESLRKMAADVETIYYVYVISLNGDLVGVLSLRDILLSNPETRVTEIMHTHIIKADVLEDQHEVAQTIAKYNLLALPVVEDETKLRGIITVDDAIDIVLPTAWKKRVPRMFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y1</accession>
    <name>F0T7Y1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0216"/>
    <dbReference type="EnsemblBacteria" id="ADZ08468">
        <property type="protein sequence ID" value="ADZ08468"/>
        <property type="gene ID" value="Metbo_0216"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0216"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_2_2"/>
    <dbReference type="OMA" id="MPVRIYE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004425">
        <property type="entry name" value="CHP00061"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00161">
        <property type="entry name" value="TIGR00161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="26385" checksum="FD659778D8176290" modified="2011-05-03" version="1">MVEMIETKSECCKIISEDVKDAIVIEGSPEFGLIGNILGWLLVEELGMKQIGYIDSKDFPPLAVLYKGVAIHPFRIYSSKGIVLFLSDFIVPPEVSYDMSTAIVDWMERNNSKEFITFNSMVVMEKSDSVAAAANSQDALKRLGDLEVPILPFGNINGISGSLLTKTATKNISGSCLFAEVLNQYPDPRAAASLVNVLNRMLDIQVNAEPLIKEAEQIEERLKELAKRVPNEPESPIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Y8</accession>
    <name>F0T7Y8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Methionine aminopeptidase</fullName>
            <shortName evidence="6">MAP</shortName>
            <shortName evidence="6">MetAP</shortName>
            <ecNumber evidence="6 7">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">map</name>
        <name evidence="9" type="ordered locus">Metbo_0223</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6 7">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643826.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0223"/>
    <dbReference type="EnsemblBacteria" id="ADZ08475">
        <property type="protein sequence ID" value="ADZ08475"/>
        <property type="gene ID" value="Metbo_0223"/>
    </dbReference>
    <dbReference type="GeneID" id="10276651"/>
    <dbReference type="KEGG" id="mel:Metbo_0223"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OMA" id="NEIAAHY"/>
    <dbReference type="OrthoDB" id="82853at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="8">
        <location>
            <begin position="4"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ08475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="304" mass="33714" checksum="07271A87255B685C" modified="2011-05-03" version="1">MIEAYEKSGKIVSKVRKMATDYIEADMKILELVDFVESNIIEMGAKPAFPCNISINEITAHYTAPPGDESIIKDGDLVKIDLGAHVDGYIADSAITVLVGDSDTVLTETENELNHKLIQTAQDALESAINTIKAGVELGEIGTAIEETITQNGLNPVSNLTGHGMDQYILHSGISVPNIKEVNDHKVEEGDVLAIEPFVTNGVGWVSDIPEVHIFRFLRDRPMRMVQAKKLLHSIAMDFRNLPFCQRWLTNENNEKHINMAMRQLINSRAIYPYHVLKEKSDARVAQAEHTVIVEADGCKVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z7</accession>
    <name>F0T7Z7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="8">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ogt</name>
        <name evidence="11" type="ordered locus">Metbo_1383</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 8">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644974.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1383"/>
    <dbReference type="EnsemblBacteria" id="ADZ09623">
        <property type="protein sequence ID" value="ADZ09623"/>
        <property type="gene ID" value="Metbo_1383"/>
    </dbReference>
    <dbReference type="GeneID" id="10277834"/>
    <dbReference type="KEGG" id="mel:Metbo_1383"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="OMA" id="MTYAYKL"/>
    <dbReference type="OrthoDB" id="122341at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA dealkylation involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008332">
        <property type="entry name" value="MethylG_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02870">
        <property type="entry name" value="Methyltransf_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_1N" evidence="10">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_binding_1" evidence="9">
        <location>
            <begin position="91"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="8">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="190" mass="21044" checksum="8B5E1D3B09D81584" modified="2011-05-03" version="1">MVKKDRTEREKEIYSTYYSSPIGSLMIASDGDNIIGLWMEGQKYFANTLTETITEKDDLEIFDTANKWLDKYFACENPPISDLPLAPRGGEFRKVVWKILREIPYGEVITYGEIAKKVAIQMNKENMSSQAVGGAVGHNPIAIIVPCHRVVGTNGSLTGYAGGIDKKVKLLEHEEVDMSGLFIPAKSTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T817</accession>
    <name>F0T817_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Big_5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1403</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09643">
        <property type="protein sequence ID" value="ADZ09643"/>
        <property type="gene ID" value="Metbo_1403"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1403"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893153_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Big_5" evidence="3">
        <location>
            <begin position="62"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="311" mass="34916" checksum="4F99592CBA87CC57" modified="2011-05-03" version="1">MNFLDKIKFRRGRGIISIIMEYLNGIKGLKTSKIMLFALVLIGMVLIFSNGMGDASAVSVNDTYKPTISAVDPANNAVGISTSKTVKLTFSEAVKKGNMWIEFKNSKGQAVQFTSNLTGKTLYIKPKSQLAHQTSYTVTLHSGSIKDLAGNGIALYSTKFTTVKATKTYSDGQIKFNYPATWHLDSDLKDGDKILFGMKYYGTLSPQFQLEIQKTPYGMSDQDMEASMIYTEYPKGYTIISKQWYTLNGNRVYEEVCTIKPDKYCPVTLENKEMTIIKNHKIYTFDFTAPLKTFSNSKTDFNTIAQSFKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T832</accession>
    <name>F0T832_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase beta</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">Metbo_1419</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">AL-21</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645009.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1419"/>
    <dbReference type="EnsemblBacteria" id="ADZ09658">
        <property type="protein sequence ID" value="ADZ09658"/>
        <property type="gene ID" value="Metbo_1419"/>
    </dbReference>
    <dbReference type="GeneID" id="10277870"/>
    <dbReference type="KEGG" id="mel:Metbo_1419"/>
    <dbReference type="eggNOG" id="arCOG00305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017729_1_0_2"/>
    <dbReference type="OMA" id="HNVRMRQ"/>
    <dbReference type="OrthoDB" id="12404at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="DNA_pol_b-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022311">
        <property type="entry name" value="UCP005047_YshC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005047">
        <property type="entry name" value="UCP005047_YshC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="SSF47802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="POLXc" evidence="20">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="18">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="18">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="18">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="POLIIIAc" evidence="19">
        <location>
            <begin position="337"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ADZ09658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="569" mass="63942" checksum="6BBC8B12A92D432A" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T840</accession>
    <name>F0T840_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645017.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1428"/>
    <dbReference type="EnsemblBacteria" id="ADZ09666">
        <property type="protein sequence ID" value="ADZ09666"/>
        <property type="gene ID" value="Metbo_1428"/>
    </dbReference>
    <dbReference type="GeneID" id="10277879"/>
    <dbReference type="KEGG" id="mel:Metbo_1428"/>
    <dbReference type="eggNOG" id="arCOG04432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_560960_0_0_2"/>
    <dbReference type="OMA" id="FYGPTCV"/>
    <dbReference type="OrthoDB" id="8224at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018694">
        <property type="entry name" value="DUF2193"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09959">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="499" mass="55724" checksum="8D94BF6CBD695ECA" modified="2011-05-03" version="1">MSELYEKMVKEAMAAQRADVDTIKKNRGGKFKITDTKAYLDVVNEMSVADGQSESVIDLHVKSVNSHYDALVNLTDTIRPEDDPFVEHYQTPAVLEILYENDKAFKDSVDKFIEAVGNAESIIGREVVRRYGGFYGPTCVVDFALIPGSTSNIVNRILKHTDIPDAHKQAILSAKSWGMNTSYGFGETFTDQIEEGMTLTDALDKEIAQIQYIYESPVEAQAKLMDGAGHESFDVRKYMAKYKKSMEETVLNAVEDGVHYGNIVTVPAYCVGDIAHHIAQSTYNMCKDDVIMAVIEAATDVMDSTLKKALPKFKSEYELLSLATGSSACAVEYMLELDGFNAPMVVDMLTKRFHNYVQLYPTRGAAAELHNCDFMDMIYRGWKYMDTERRMKNGSGRNLEPDVAGFKVDLTPVHKNNVIMNPQRYAYPACAITVRYSALMRLADYPCLLTSEPVTATMMTNIIALHKESPASPARVCKNCASACLVDFRHCACQWKEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T866</accession>
    <name>F0T866_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643829.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0226"/>
    <dbReference type="EnsemblBacteria" id="ADZ08478">
        <property type="protein sequence ID" value="ADZ08478"/>
        <property type="gene ID" value="Metbo_0226"/>
    </dbReference>
    <dbReference type="GeneID" id="10276654"/>
    <dbReference type="KEGG" id="mel:Metbo_0226"/>
    <dbReference type="eggNOG" id="arCOG03300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136765_3_1_2"/>
    <dbReference type="OMA" id="RRDINQF"/>
    <dbReference type="OrthoDB" id="108821at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012427">
        <property type="entry name" value="DUF1622"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38468">
        <property type="entry name" value="PTHR38468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07784">
        <property type="entry name" value="DUF1622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="12112" checksum="7E7DF3EBC22E96B8" modified="2011-05-03" version="1">MYSDIVNYVVLFFSIFGAILIVYGGLRAIFNVLKVELWKHSSDYNSIRIDFTSKIVLALEFFIAADLVRTIIRPDLNEVIVLAVIVAIRTVVGYSLDKEAKDILSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T867</accession>
    <name>F0T867_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643830.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0227"/>
    <dbReference type="EnsemblBacteria" id="ADZ08479">
        <property type="protein sequence ID" value="ADZ08479"/>
        <property type="gene ID" value="Metbo_0227"/>
    </dbReference>
    <dbReference type="GeneID" id="10276657"/>
    <dbReference type="KEGG" id="mel:Metbo_0227"/>
    <dbReference type="eggNOG" id="arCOG11916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207628_0_0_2"/>
    <dbReference type="OrthoDB" id="129297at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021315">
        <property type="entry name" value="Gap/Sap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11139">
        <property type="entry name" value="SfLAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="24578" checksum="8600BD39C7FDCDC6" modified="2011-05-03" version="1">MSGTVSLLAQIIPLAFGAAVSPTALMGIILLLSISKKPKISGMSYYAGSILMVLIVLCVGFILGNGVSSESPHPNPLLAAIDLILGVILLIMGIIRIFRPQKSPKHRFNGEHPDSSSLLVFLKGLSFGFVMFFINFSTTLIVLEAGKLIATAAVPITGKVTVSVILILITLLVCEVPLLIYFLLPGRSEKLLSKINVWMQKNGHILMGLVILAIGLYLIWIGSIKFGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T874</accession>
    <name>F0T874_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ThiJ/PfpI domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643837.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0234"/>
    <dbReference type="EnsemblBacteria" id="ADZ08486">
        <property type="protein sequence ID" value="ADZ08486"/>
        <property type="gene ID" value="Metbo_0234"/>
    </dbReference>
    <dbReference type="GeneID" id="10276664"/>
    <dbReference type="KEGG" id="mel:Metbo_0234"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_5_2"/>
    <dbReference type="OMA" id="DAWYRLF"/>
    <dbReference type="OrthoDB" id="83832at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="23511" checksum="3E0EAB2C449F9C93" modified="2011-05-03" version="1">MKAYLYVLNTLADWEIGYLTAELNSGRYLDKTRPSVELIKIGNTTEPIKTMGGITIVPDESIDNIGFKEDDLLILPGADTWMEEENKKIMDIVSDIIDKKVIIAAICGATIALANKGILNDRNHTSNDIEVLKMFCPEYSGENFYKNQPAVTDDNLITASGITPLEFSYEILKRINVMKAETLEAWYQLYKTNESKYFYALMESIKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T896</accession>
    <name>F0T896_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="2 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="10" type="ordered locus">Metbo_0256</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="2 9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643859.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0256"/>
    <dbReference type="EnsemblBacteria" id="ADZ08508">
        <property type="protein sequence ID" value="ADZ08508"/>
        <property type="gene ID" value="Metbo_0256"/>
    </dbReference>
    <dbReference type="GeneID" id="10276688"/>
    <dbReference type="KEGG" id="mel:Metbo_0256"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_2"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="49443at2157"/>
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        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
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        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
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        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="393" mass="44143" checksum="4D63371DBDDEC7B4" modified="2011-05-03" version="1">MEKVVLAFSGGLDTSVCVKLLQEKYNMEVVTACVDVGQPEDEIERPAQVANNIGVKKHYTIDAKHEFAEEFIFRAIKANAMYEGYPLSTSLARPLIAMKIVELAKKEQADAIAHGCTGKGNDQFRFETTIRSSSVCDIIAPVRDLNLTRTEEMDYAVKNDIPVPSDKLYSIDENLWGRSIEGDILEDPMVETPEEAFEWTQSTENAPDEPQKLTVTFEEGVPVAIDGEMMNALEIIQEANRIAGLHGIGRVDIIEDRIIGLKSRENYETPGAELLITAHKALEQLTLTREELKFGETVTQAYSELVYNGLWHEPLREDLDCIIDNMQLRVSGDVILKLHKGSIRILARESPYSLYSEEAVSFEDKEMDQREIAGMVKNYGLQAACYQKVCKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8B1</accession>
    <name>F0T8B1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643874.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0271"/>
    <dbReference type="EnsemblBacteria" id="ADZ08523">
        <property type="protein sequence ID" value="ADZ08523"/>
        <property type="gene ID" value="Metbo_0271"/>
    </dbReference>
    <dbReference type="GeneID" id="10276703"/>
    <dbReference type="KEGG" id="mel:Metbo_0271"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108340_0_0_2"/>
    <dbReference type="OMA" id="YCHDFCP"/>
    <dbReference type="OrthoDB" id="108931at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="17995" checksum="7C6A900881144369" modified="2011-05-03" version="1">MKNLIRIFLDGIINNTKRIIFASDRVTDMELRSKILEGRVVPTEKVAEQSCIGCGGCSNACPTKAIEMVDLPEPVELMEGLVKDKIPVLNSLKCVNCYYCHDFCPLYALFGEAGTIHPNDVGIVESDISELLDKPVKISDDKVAFISQYLADSTILKKRKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C3</accession>
    <name>F0T8C3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0283"/>
    <dbReference type="EnsemblBacteria" id="ADZ08535">
        <property type="protein sequence ID" value="ADZ08535"/>
        <property type="gene ID" value="Metbo_0283"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0283"/>
    <dbReference type="eggNOG" id="arCOG10257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184180_0_0_2"/>
    <dbReference type="OMA" id="DIMSSCR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="85" mass="9454" checksum="6908A6854853A60C" modified="2011-05-03" version="1">MKKCESMDDTSVMGSCEVLLNMMKDKNVTLDEDPNQTYLGMAENLTPKDVSKVLQLALKVRESGDITDTELKNAASRLIRAIEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8H9</accession>
    <name>F0T8H9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1498</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645081.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1498"/>
    <dbReference type="EnsemblBacteria" id="ADZ09730">
        <property type="protein sequence ID" value="ADZ09730"/>
        <property type="gene ID" value="Metbo_1498"/>
    </dbReference>
    <dbReference type="GeneID" id="10277949"/>
    <dbReference type="KEGG" id="mel:Metbo_1498"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="OrthoDB" id="32313at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="PTHR23264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="285" mass="31009" checksum="DD6BEE740E0B7A95" modified="2011-05-03" version="1">MATEEKELKYSAEEEHKIKLMQQEVDIVRRMSKIKHKIAVMSGKGGVGKSTIAVNLAAAFAKKGYKTGIMDVDIHGPNVPMMLGVEGKHLIFTSDGIQPVETEGIKIMSVGFFLDSLDSPVIWRGPKKTGVIKQFLSEVNWGDLDVLIIDNPPGTGDEPLTILQSVPLDGVVLVTTPQAVVQEDVRKGINLVLDSKVPIIGIVENMSGFICPHCNEEVPIFGSGNTEEMAKKMDVHFLGKLPLNVETPVSSDTGTPIVIKDPNSDIAIKISEIANYIEKTVIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I2</accession>
    <name>F0T8I2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645084.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09733">
        <property type="protein sequence ID" value="ADZ09733"/>
        <property type="gene ID" value="Metbo_1501"/>
    </dbReference>
    <dbReference type="GeneID" id="10277952"/>
    <dbReference type="KEGG" id="mel:Metbo_1501"/>
    <dbReference type="HOGENOM" id="CLU_1840583_0_0_2"/>
    <dbReference type="OrthoDB" id="140702at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="139" mass="14929" checksum="436624BD54092666" modified="2011-05-03" version="1" precursor="true">MNNTGKTLIIIIIALVAVLSLTVGFVLQGNLSTKNTTDLNTTTINQTTQNVTTKSTNQSSSIISSSEAVAIAKKWGASKGMEPDGYVSYLSGKGMYQGADGNPFYHVGMKWIDPNRTVQEYGDAGYVEIDAKTGKMVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8J7</accession>
    <name>F0T8J7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645099.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1517"/>
    <dbReference type="EnsemblBacteria" id="ADZ09748">
        <property type="protein sequence ID" value="ADZ09748"/>
        <property type="gene ID" value="Metbo_1517"/>
    </dbReference>
    <dbReference type="GeneID" id="10277968"/>
    <dbReference type="KEGG" id="mel:Metbo_1517"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_0_0_2"/>
    <dbReference type="OMA" id="ELIMANY"/>
    <dbReference type="OrthoDB" id="106121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="92" mass="10708" checksum="9552776A6548AC2B" modified="2011-05-03" version="1">MDKLIWWVFAGTAGGPNRARIVRALNERPYNANQLAEKLNLNYKTIRHHLKTLEENKMIDSSGEKKYGTLYFLTSGMEENYHVLNEIIKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K2</accession>
    <name>F0T8K2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645104.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1523"/>
    <dbReference type="EnsemblBacteria" id="ADZ09753">
        <property type="protein sequence ID" value="ADZ09753"/>
        <property type="gene ID" value="Metbo_1523"/>
    </dbReference>
    <dbReference type="GeneID" id="10277974"/>
    <dbReference type="KEGG" id="mel:Metbo_1523"/>
    <dbReference type="HOGENOM" id="CLU_1860716_0_0_2"/>
    <dbReference type="OrthoDB" id="12426at183925"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="14852" checksum="1654D20B8D85C0C1" modified="2011-05-03" version="1">MESNNSKSIKAPLTGAMKMLAVLFGIGFILGFILTPLGVETRSPEIRTLLFGVFFIVIGLLIPLVGLVLIWLRKNKFAGVLAVVGAAFIFLVAPADQALFFFTVSPPPAVTIGEFILIFVGIGYMLYGPKVYDEEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8M1</accession>
    <name>F0T8M1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holliday junction resolvase Hjc</fullName>
            <shortName evidence="10">Hjc</shortName>
            <ecNumber evidence="10">3.1.21.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hjc</name>
        <name evidence="11" type="ordered locus">Metbo_0311</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643914.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0311"/>
    <dbReference type="EnsemblBacteria" id="ADZ08563">
        <property type="protein sequence ID" value="ADZ08563"/>
        <property type="gene ID" value="Metbo_0311"/>
    </dbReference>
    <dbReference type="GeneID" id="10276743"/>
    <dbReference type="KEGG" id="mel:Metbo_0311"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139546_0_0_2"/>
    <dbReference type="OMA" id="WYFFHPG"/>
    <dbReference type="OrthoDB" id="97278at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="archeal_HJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="PTHR39651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="15406" checksum="0A67BEE127B4B457" modified="2011-05-03" version="1">MSKTGSREERELVRMLWDADCAAMRAPASGGATKKPLPDVIAGNGSIYLAIEVKSSSLERIYIDSEKIDGLKEFSQIFGAQPYIGVKFLRKKWRFICLEDLHITRNNNYRVNIDLAFDKGLDFDEILGNDKQVKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Q5</accession>
    <name>F0T8Q5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643948.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0345"/>
    <dbReference type="EnsemblBacteria" id="ADZ08597">
        <property type="protein sequence ID" value="ADZ08597"/>
        <property type="gene ID" value="Metbo_0345"/>
    </dbReference>
    <dbReference type="GeneID" id="10276782"/>
    <dbReference type="KEGG" id="mel:Metbo_0345"/>
    <dbReference type="eggNOG" id="arCOG02614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521882_0_0_2"/>
    <dbReference type="OMA" id="VTTPMCE"/>
    <dbReference type="OrthoDB" id="112822at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="176" mass="19913" checksum="4863E38F39B94C49" modified="2011-05-03" version="1">MKIVTTPMCEDILKLAGLKEYQVSLNPDSTDADIAVVLSETNTGMQSIKLKLNTFKQIQESIEIVQTKLGTGSIGEGSSIQLPHGVNTRNRNIKVKVYSNFLRDIVEDMGYTVVNGNEHHDFLVYPDYIRDEIQGELDSMGENYVEITSHKNSPINPVKRAELRYKLLEKKLCMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S2</accession>
    <name>F0T8S2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ADZ08614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0362"/>
    <dbReference type="EnsemblBacteria" id="ADZ08614">
        <property type="protein sequence ID" value="ADZ08614"/>
        <property type="gene ID" value="Metbo_0362"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0362"/>
    <dbReference type="eggNOG" id="arCOG00238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_0_1_2"/>
    <dbReference type="OMA" id="NFSICLM"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="344"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="465" mass="49394" checksum="B30055349497D4A3" modified="2011-05-03" version="1">MWEGCKTNLSFKTLGMPLAIVAFFLVMIIPLGDGLSYSGHAALALLAFAIIMWASEAQHLAVTSLILLFLQPILGIESFNNAVIGFANPIIFLMIGGFILAEAIRKSGLAKRFTYFMLKKVGTTPSMSIFAAVFSTGLLSAWIENVVAFAMLLPIIKEIVPLFGVNDAEKGNSNFAKAMILGASYGSLAGGFGTEIGTAPNLMAAAYTHLPFVQWMIFGIPLSIIMLFVTWKLLSWIFPPEVKGIIGGHETLNKTLSAMGSISRTEKITAVILFFTIGLWVTTGITGIDSYSVALIGAALYFITGVVDWKDAQENVDWGLIIFFGGALSLGAALLNTGAAAWLINDLVTLMGNNVSTVSIMLLLMIIGVIFTQVMSNIALSAILVPLSVTLAAAQGQPIGVYAVPVAIACSLSFMFPMADPTVAMAYGTGFVKIKEILKAGIPMVVIGIILTVVIMLTIAQPFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U1</accession>
    <name>F0T8U1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645120.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1539"/>
    <dbReference type="EnsemblBacteria" id="ADZ09769">
        <property type="protein sequence ID" value="ADZ09769"/>
        <property type="gene ID" value="Metbo_1539"/>
    </dbReference>
    <dbReference type="GeneID" id="10277994"/>
    <dbReference type="KEGG" id="mel:Metbo_1539"/>
    <dbReference type="eggNOG" id="arCOG10948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165284_0_0_2"/>
    <dbReference type="OrthoDB" id="121718at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="110" mass="12443" checksum="4DE8A08684E58E16" modified="2011-05-03" version="1">MFMDLNLLGFSNVLPSLFILVKSSPLITSVANICLLIGMLYVYLERYQKVKSKFTTTLVLFSLLFLIQNIIFSVYLIYYPPVTIEAAALPLIFLGLEFAALALLLMITRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W9</accession>
    <name>F0T8W9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645148.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1570"/>
    <dbReference type="EnsemblBacteria" id="ADZ09797">
        <property type="protein sequence ID" value="ADZ09797"/>
        <property type="gene ID" value="Metbo_1570"/>
    </dbReference>
    <dbReference type="GeneID" id="10278026"/>
    <dbReference type="KEGG" id="mel:Metbo_1570"/>
    <dbReference type="eggNOG" id="arCOG06469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782509_0_0_2"/>
    <dbReference type="OMA" id="PLVWNIN"/>
    <dbReference type="OrthoDB" id="111239at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="15421" checksum="8216E62F192F4DB7" modified="2011-05-03" version="1" precursor="true">MKWGLKNISLVLGVLIVLAIVGVSGCTSSGNSTSDQPKINVTNLTVKSSGYGGYDINAVVVPNKDIGYLEMATVWYDSSGAIIEKSPLVWNINNVTAGQVYKVNGMDSLYDKGTPAKVDVYIFDSVFSGGDTSDAIYHATVNMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8Y7</accession>
    <name>F0T8Y7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1588</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645166.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1588"/>
    <dbReference type="EnsemblBacteria" id="ADZ09815">
        <property type="protein sequence ID" value="ADZ09815"/>
        <property type="gene ID" value="Metbo_1588"/>
    </dbReference>
    <dbReference type="GeneID" id="10278045"/>
    <dbReference type="KEGG" id="mel:Metbo_1588"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="ENGTIMM"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="283"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="40726" checksum="FD762BF800E5F6DF" modified="2011-05-03" version="1">MLDIAFKDFKAKKSRAAMCIIGVMACVLLIGVINVVMYEMVSGLKGDLGTVHGKMYFEQNGTSYPPAGSVITESLGNEVLNNSVVDSQKSTNALFTPLQGSQTDDGGMTMVVGLSPGREDAFIGNATVTGKSSLVGEADNAVIIGSETAKTYNSTVGSTITVNNETFKVVGIMKKVGSGWPLTIDSSMVMSLAHAQNISDRKGLVSTVIMVPSNGNSQQTVEDTLQSAYPKYDVYTEKDTQKTIDNQLKEVMIFMNMVIIMIFAVSMVLIMNVMMMSVKEKTKDIGTMRALGTKKRSIMLLIIYESLILSSIGGIIGILLISPVYSLLGILMGATNFSFVLPTSVIVQVAVIVLVIGTFSGLLPAYLANKISPIEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T916</accession>
    <name>F0T916_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="7 19">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="20" type="ordered locus">Metbo_0386</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AL-21</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Cell membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="7 19"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643989.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0386"/>
    <dbReference type="EnsemblBacteria" id="ADZ08638">
        <property type="protein sequence ID" value="ADZ08638"/>
        <property type="gene ID" value="Metbo_0386"/>
    </dbReference>
    <dbReference type="GeneID" id="10276823"/>
    <dbReference type="KEGG" id="mel:Metbo_0386"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="OMA" id="CACCGIP"/>
    <dbReference type="OrthoDB" id="108934at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ADZ08638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="27301" checksum="90B3832C099713F8" modified="2011-05-03" version="1">MTDNKVDDGIGTSIRGFLGLISFSTILPLNIHTTIEEMASFTWVWPMIGAMIGLVVGCFGYATMDLLHLSPFIAAALIYSFSIWFTGFHHLDGLIDFGDGMMVHGTPEKKIDVMRDKRIGTGGIAYLLMAGLITFAAIGSAHAALIFYILIISEMAGKMGIVGCATVSKPFPNGTGKSFIVSMSKTRLVLSVVLTSAVGFLIFNTTGVIGVLTGMTAGFLVAIVARKNFVYATGDVLGTSNEVARMLAILVMVALTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T958</accession>
    <name>F0T958_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644031.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0428"/>
    <dbReference type="EnsemblBacteria" id="ADZ08680">
        <property type="protein sequence ID" value="ADZ08680"/>
        <property type="gene ID" value="Metbo_0428"/>
    </dbReference>
    <dbReference type="GeneID" id="10276865"/>
    <dbReference type="KEGG" id="mel:Metbo_0428"/>
    <dbReference type="eggNOG" id="arCOG09718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_888340_0_0_2"/>
    <dbReference type="OMA" id="SGVWMLT"/>
    <dbReference type="OrthoDB" id="42248at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="299" mass="32900" checksum="E31ECCED86AE8AC4" modified="2011-05-03" version="1">MDTEKFLNSIGIGNQNNEPSAKRFPDGAQYRFEVPGIQNPNAMAALFEATDSYGVNIHRVTQTKGIMLLTDAEIEEMVTIAADEKVELFLSVGPRATYDTSASANTPEGARIGYRLRGYKNLFYGLEDVKRAVALGVRGIVVYDEGLLWVLGEMRKKGELPEDVHFKVSAHCGHGNPASAKLLELIGADSFNPVRDLQIPMMAEIRRSINISLDLHMENPKSSGGFIRHYEAPEIIKAAAPVYLKTGGSVAAHHGWDTSASQAAERIKQVYLVQSMIDRYYPEAKLSKTGAEDMSIPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C4</accession>
    <name>F0T9C4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polymorphic membrane protein Chlamydia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645226.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1649"/>
    <dbReference type="EnsemblBacteria" id="ADZ09875">
        <property type="protein sequence ID" value="ADZ09875"/>
        <property type="gene ID" value="Metbo_1649"/>
    </dbReference>
    <dbReference type="GeneID" id="10278106"/>
    <dbReference type="KEGG" id="mel:Metbo_1649"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016039_0_0_2"/>
    <dbReference type="OMA" id="NNTATHG"/>
    <dbReference type="OrthoDB" id="102663at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="826" mass="88216" checksum="B1E7A6A4EEBABE1A" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9F6</accession>
    <name>F0T9F6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Daunorubicin resistance ABC transporter, inner membrane subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0452</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644055.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0452"/>
    <dbReference type="EnsemblBacteria" id="ADZ08704">
        <property type="protein sequence ID" value="ADZ08704"/>
        <property type="gene ID" value="Metbo_0452"/>
    </dbReference>
    <dbReference type="GeneID" id="10276890"/>
    <dbReference type="KEGG" id="mel:Metbo_0452"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_2_3_2"/>
    <dbReference type="OMA" id="VMITMYT"/>
    <dbReference type="OrthoDB" id="77595at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005942">
        <property type="entry name" value="Daunbcin-R_ABC-transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01247">
        <property type="entry name" value="drrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="7"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="254" mass="27658" checksum="FCE3CE668F72418D" modified="2011-05-03" version="1">MAEWEGIYTIWFREAKRYVRYRSRIISSVVTPLLWLLIFGTGLGSAIRFSGMTGAYQAFIYPGIIGQTILFTAIFSGVSVIMDRQYGFLKEIMVAPVSRPSIVFGKALGIATASIIQGVILLLLSFVVGVQMTPFIFVASFLLMIPISVGLAGVGLLIATFTDSMEGFNLIMSFIVLPMFLLSGALFPVTGLPTFLQFAVYLDPLTYGVDALRQVIIGHGALPLYLSTAVVVGFALLTVILSAILFSKKEQNLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9H5</accession>
    <name>F0T9H5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="8">DGGGPL reductase</shortName>
            <ecNumber evidence="8">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Geranylgeranyl reductase</fullName>
            <shortName evidence="8">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_0471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644074.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0471"/>
    <dbReference type="EnsemblBacteria" id="ADZ08723">
        <property type="protein sequence ID" value="ADZ08723"/>
        <property type="gene ID" value="Metbo_0471"/>
    </dbReference>
    <dbReference type="GeneID" id="10276909"/>
    <dbReference type="KEGG" id="mel:Metbo_0471"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OMA" id="CSCAQYE"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="9">
        <location>
            <begin position="6"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="281"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="285"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ08723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="385" mass="41559" checksum="7478B090077B0D60" modified="2011-05-03" version="1">MTKLKYDVVVVGARVGGSTSALFASKSDVDVLMLEKRQEIGSPVQCAEGVTYNTFETLKIKPNEKYIRSKIKGANIHAPDGRCINYEGGVAEGLVLDRKVFDKELASEAAKSGSDIMVKTAVKDLIIKDHKVCGVIAKHLGETIEVKADVVIAADGIESNMARMAGLKTIQSPQQICSCAQYEMVGVDVDPNYLEFYFGENVAPKGYVWIFPKGDGVANVGVGIRSPGKTPLYYLDKFVSKLDATPVELNIGGVPVSGPIEKTYTDGLLVVGDAAGHVDPITGGGIHPTITCARIAGEVAAESVKNEDCSSNYLKNYEKLWKKEVGKGLSQSAKYRKMADKLSDKDMNSLAEFIENQDIESISKIAALKFVGKNPNLMKLLTEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M5</accession>
    <name>F0T9M5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644124.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0521"/>
    <dbReference type="EnsemblBacteria" id="ADZ08773">
        <property type="protein sequence ID" value="ADZ08773"/>
        <property type="gene ID" value="Metbo_0521"/>
    </dbReference>
    <dbReference type="GeneID" id="10276966"/>
    <dbReference type="KEGG" id="mel:Metbo_0521"/>
    <dbReference type="eggNOG" id="arCOG01880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058561_7_0_2"/>
    <dbReference type="OMA" id="MAWGDGL"/>
    <dbReference type="OrthoDB" id="83735at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="23824" checksum="6029700C9EE30A47" modified="2011-05-03" version="1">MISSDVTGLILVYSYVALILIVSEKVLGKYTTFSRKFLHIMVGNVIFILPFFTDQFVITFLAAAPFVVLTFLISPYSPVKINDKISSSGHGMGLVYYAISWTVLAFFFYDQPWIIAVGIAAMSYGDGFAALIGERYGKHKFKISSDTKSVEGSLGMFLVLMVSLFIVLIYFNVTVPSLLVIPCVAITATIFEAVTPKGLDNLSACFAAVGVYILTTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R1</accession>
    <name>F0T9R1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645291.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1716"/>
    <dbReference type="EnsemblBacteria" id="ADZ09940">
        <property type="protein sequence ID" value="ADZ09940"/>
        <property type="gene ID" value="Metbo_1716"/>
    </dbReference>
    <dbReference type="GeneID" id="10278173"/>
    <dbReference type="KEGG" id="mel:Metbo_1716"/>
    <dbReference type="eggNOG" id="arCOG01624">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_3_2_2"/>
    <dbReference type="OMA" id="CMRLPIL"/>
    <dbReference type="OrthoDB" id="43721at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="304"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="383" mass="43887" checksum="E7BA48DE7EE13EB4" modified="2011-05-03" version="1">MLKRKFGKTGERVSILGFGCMRLPILDGNPEKINEPLAREMLYHAIDEGVNYVDTAFPYHGTSAQGGGMSEVFLGNALRNNYREDVFLSTKLPSWLVQKKEDLNFFLDEQLKRLQTDRIDFYLLHGLHQDFWQTLLLADVFDFLDNAIDEGKIGYAGFSFHDEYEFFKLVVDSYDWSFAQIQLNYMDEKFQAGKAGLDYAKNQDLGTVIMEPLRGGCLTKNLPEEVKSIWNGAENKKSPAEWGLRYLWNQPEVNVVLSGMSTMEQLNENLEIAKRGHVNSLTHDELELYEKVKNVYAERVHVGCTSCNYCMPCPKGVDIPLNLSLLNDVYMYKNLEKPTGNYKFLMGKKMSAGYCNQCGDCEKKCTQNLEVTSYLQENVDLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z1</accession>
    <name>F0T9Z1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ordered locus">Metbo_0563</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0563"/>
    <dbReference type="EnsemblBacteria" id="ADZ08814">
        <property type="protein sequence ID" value="ADZ08814"/>
        <property type="gene ID" value="Metbo_0563"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0563"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="OMA" id="HAHGDNM"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="PTHR39648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MAF_flag10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MAF_flag10" evidence="6">
        <location>
            <begin position="69"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="30537" checksum="DE45C6DE33F5BFA2" modified="2011-05-03" version="1">MRRSKYHLLNKLYLVLNQPNCMNLVQWFSWYDEILADFGFDRSADEESAKLLQNLLGDDGLSPTDIKIKENVVIFGAGPSLKENVLEFKQINMENFSVICADGAVTALLEEDIVPEIVVTDLDGNIDDLLTSNHQGSIMVVHAHGNNMETLKKYVPKLENVLGTTQSTPLKNIYNFGGFTDGDRCLFLAGKLKAKNIILAGMDFGKVVTKYSRPDIDGELGHADSIKEKKLQYAKKLVEWMVENEEITICNVSNGETISMVKNIEFSDIPISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA02</accession>
    <name>F0TA02_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420 hydrogenase</fullName>
            <ecNumber evidence="4">1.12.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.98.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644176.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0574"/>
    <dbReference type="EnsemblBacteria" id="ADZ08825">
        <property type="protein sequence ID" value="ADZ08825"/>
        <property type="gene ID" value="Metbo_0574"/>
    </dbReference>
    <dbReference type="GeneID" id="10277019"/>
    <dbReference type="KEGG" id="mel:Metbo_0574"/>
    <dbReference type="eggNOG" id="arCOG02650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_786694_0_0_2"/>
    <dbReference type="OMA" id="CMDFTSE"/>
    <dbReference type="OrthoDB" id="41902at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="367" mass="41058" checksum="CE9E6431E6EDCCBA" modified="2011-05-03" version="1">MENSSKEKVAVVGTPCHMVAASKLEKFSDVLGNSPIDIKIGLFCMENFSYSSIKKLLDKNNIDMNDIQECRVEKNYMWFYLNEDKIFKVPLNKAKSCIRKNCNICMDFTSELSDISVGSVGSEEGWSTVIVRSDKGLEFLKNAEKDSYIETKPIDEKGIKLIENLSNKKMKENIEEIQRRELVGRPVIYRRQMPIREYYEEIENCQFNDLKGDVIDIGACVLCGACDYVCPEGIIKIDDRKPQLTGKCPEGCNLCYMACPRTYVPDDIMTTDADKKPLGDYLKIVSAKAPMLNGQDGGVATALLNYALAKNVVDDVIVVDKNSKEPWKPEPKLTNNVADVLKAAGTKYSACTIFTPLKDLNKGKGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA10</accession>
    <name>F0TA10_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ordered locus">Metbo_0582</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AL-21</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644184.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0582"/>
    <dbReference type="EnsemblBacteria" id="ADZ08833">
        <property type="protein sequence ID" value="ADZ08833"/>
        <property type="gene ID" value="Metbo_0582"/>
    </dbReference>
    <dbReference type="GeneID" id="10277027"/>
    <dbReference type="KEGG" id="mel:Metbo_0582"/>
    <dbReference type="eggNOG" id="arCOG04180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="OMA" id="YMRRHGP"/>
    <dbReference type="OrthoDB" id="21175at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="PTHR38341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="SSF111249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="FBP binding" evidence="15">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="FBP binding; shared with dimeric partner" evidence="15">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADZ08833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="365" mass="40254" checksum="0138A67D7F7B9EE6" modified="2011-05-03" version="1">MKTTISVIKADVGSIAGHAKTHVALKNKCDEVLAKAKESELLVDYHITSCGDDIDLIMTHRNGEENEEVHELAWNAFLEATQVAKSLKLYGAGQDLLSDTFSGNIKGMGPGCAEMEFKERPSDPVIIFCCDKTEPGAFNMPLFKMFADPFNTAGLVIDPSLHGGYDFEIFDVIEHKKVTMSCPDEMYDALALLGSTGRYVIKHISRRSDNEIAASVSTERLNLMAGQYIGKDDPVAVVRAQSGFPAAGEVVEPFAFPHLVSGWMRGSHNGPLMPVAQRNANPVRFDGPPRVIALGFQINNSELVGPLDMFDDPAFDRSRDLASEVAEYMRRHGPFEPHRLPADEMEYTSLPGVLKMLEDRMEDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA85</accession>
    <name>F0TA85_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645386.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1813"/>
    <dbReference type="EnsemblBacteria" id="ADZ10035">
        <property type="protein sequence ID" value="ADZ10035"/>
        <property type="gene ID" value="Metbo_1813"/>
    </dbReference>
    <dbReference type="GeneID" id="10278270"/>
    <dbReference type="KEGG" id="mel:Metbo_1813"/>
    <dbReference type="HOGENOM" id="CLU_2949308_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="6862" checksum="4A7F19835022A2E6" modified="2011-05-03" version="1">MLEHTYKYQNYLRNNSPCFGLFYTAFVVALEQQIEILDKSMGYTAPITSNINEMAPMSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA90</accession>
    <name>F0TA90_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nitrogenase protein alpha chain</fullName>
            <ecNumber evidence="8">1.18.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1818</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[7Fe-Mo-9S-C-homocitryl] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30409"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[8Fe-7S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21143"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645391.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1818"/>
    <dbReference type="EnsemblBacteria" id="ADZ10040">
        <property type="protein sequence ID" value="ADZ10040"/>
        <property type="gene ID" value="Metbo_1818"/>
    </dbReference>
    <dbReference type="GeneID" id="10278275"/>
    <dbReference type="KEGG" id="mel:Metbo_1818"/>
    <dbReference type="eggNOG" id="arCOG00598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025876_1_0_2"/>
    <dbReference type="OMA" id="VDIFVCN"/>
    <dbReference type="OrthoDB" id="10827at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005974">
        <property type="entry name" value="Nase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010143">
        <property type="entry name" value="Nase_comp1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011290">
        <property type="entry name" value="Nase_Fe-Fe_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43457">
        <property type="entry name" value="PTHR43457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01284">
        <property type="entry name" value="alt_nitrog_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01861">
        <property type="entry name" value="ANFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01862">
        <property type="entry name" value="N2-ase-Ialpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00090">
        <property type="entry name" value="NITROGENASE_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="7" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="9">
        <location>
            <begin position="49"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="509" mass="57459" checksum="3D000989BF852B27" modified="2011-05-03" version="1">MPYHTFKCSKCIPERTKHAVIKGHDEDLTSCLPLGYLNTIPGSISERGCAFCGAKHVIGTPMKDVIHLCHGPVGCTYDTWQTKRYISDNDNFQFKYTFATDMKEKHIVFGSEKLLKESITEAFKAFPEINRMAIYQTCASALIGDDMDAVAQEVMNEMPDVDIFVCNSPGFAGPSQSGGHHKINIAWVNQKVGTVEPKITSDYVINYVGEYNIQGDQEVMTDYFKRMGIQVLSTFTGNGSYDDLRSMHKADLNVLECARSAEYICNELRVRYGTPRMDIDGFGFKPLSESLLKVGLFFGLEEEAQKIIEEETARWKPELDWYARRLKGVKVCLWPGGSKLWHWANVIHEEMGVEVVSLYTKFGHQGDMEKGIARCGEGALAIDDPNELEGIEAMMELQPDVIFTGVRPGEVAKKLRVQYLNAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSGLDISKDEIDMNNGFVTKNIISDAKIDKEASIANNEREYTGKYDCVTHLRDKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAC2</accession>
    <name>F0TAC2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA-free ribonuclease P</fullName>
            <shortName evidence="5">RNA-free RNase P</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein-only RNase P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-free RNase P that catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HARP family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644225.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0623"/>
    <dbReference type="EnsemblBacteria" id="ADZ08874">
        <property type="protein sequence ID" value="ADZ08874"/>
        <property type="gene ID" value="Metbo_0623"/>
    </dbReference>
    <dbReference type="GeneID" id="10277068"/>
    <dbReference type="KEGG" id="mel:Metbo_0623"/>
    <dbReference type="eggNOG" id="arCOG00720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109672_0_0_2"/>
    <dbReference type="OMA" id="YIIKKPP"/>
    <dbReference type="OrthoDB" id="68994at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01078">
        <property type="entry name" value="RNA_free_RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014856">
        <property type="entry name" value="RNA_free_RNase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173">
        <property type="entry name" value="PTHR41173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08745">
        <property type="entry name" value="PIN_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03875">
        <property type="entry name" value="RNA_lig_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="220" mass="25307" checksum="D257D06BA7734130" modified="2011-05-03" version="1">MLAKQRFVLDTTAFTDTQLRDDYGEGDLSKTVDAMLDLIAKSRIKLNISCHMPPITYKEFTDYMSRYECPDETLVKAETWIVKKTPNRYDTKIPSEIFYEYVQDMRERMNKGMRISENGIWEAAVESMVMMSRGTQKSEIEAKVLGKAITDFRKRYRAALRKGTLDSAPDLDVLLLAKELGAGVVAADEGIKVWAERLGLRFLSAKSFPQMMEEYLQHYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAG7</accession>
    <name>F0TAG7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644270.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0668"/>
    <dbReference type="EnsemblBacteria" id="ADZ08919">
        <property type="protein sequence ID" value="ADZ08919"/>
        <property type="gene ID" value="Metbo_0668"/>
    </dbReference>
    <dbReference type="GeneID" id="10277113"/>
    <dbReference type="KEGG" id="mel:Metbo_0668"/>
    <dbReference type="eggNOG" id="arCOG04444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136790_2_1_2"/>
    <dbReference type="OMA" id="CDTAGFG"/>
    <dbReference type="OrthoDB" id="89042at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045739">
        <property type="entry name" value="ACT_dom_pair"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19571">
        <property type="entry name" value="ACT_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="143" mass="15736" checksum="FE65E90DDA6D6F44" modified="2011-05-03" version="1">MKVKQLSIFLENRKGRMKNALDVLETGGINIRALSIADTSDFGILRLIVPEPDRAKKLLEEHNFIVKVGEVIAVRMPDHPGGLGEILGILDGQNINLDYLYAFAEQKNEMAIVLLHPENIDSGIKALEDGGAEIITSNEIYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAJ3</accession>
    <name>F0TAJ3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645416.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1843"/>
    <dbReference type="EnsemblBacteria" id="ADZ10065">
        <property type="protein sequence ID" value="ADZ10065"/>
        <property type="gene ID" value="Metbo_1843"/>
    </dbReference>
    <dbReference type="GeneID" id="10278300"/>
    <dbReference type="KEGG" id="mel:Metbo_1843"/>
    <dbReference type="eggNOG" id="arCOG02008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1292044_0_0_2"/>
    <dbReference type="OMA" id="NNDIWGH"/>
    <dbReference type="OrthoDB" id="122250at28890"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="213" mass="23402" checksum="CCADCE66A52D7C25" modified="2011-05-03" version="1">MTNNNSRDSYQKHQKIFALCGIVAPILFTVLVIIESLLRPGYSQILNDVSDLGLGPYALIQNISFMVFGLLSIGFAMGLGANLPNRAGKAVKWLVTVFGLCIILAGVTLLSAPGDVTYAKDVVSHGLVSAVAFLLIIIAMFLTWLALRNSNNDIWGHYRLFSLICGLFSIITLVILSYTQFYSYHGASERLFIAVWMIWIEGTGLKLYSLSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAK7</accession>
    <name>F0TAK7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645430.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1858"/>
    <dbReference type="EnsemblBacteria" id="ADZ10079">
        <property type="protein sequence ID" value="ADZ10079"/>
        <property type="gene ID" value="Metbo_1858"/>
    </dbReference>
    <dbReference type="GeneID" id="10278316"/>
    <dbReference type="KEGG" id="mel:Metbo_1858"/>
    <dbReference type="eggNOG" id="arCOG01681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_7_3_2"/>
    <dbReference type="OMA" id="EGVWIHY"/>
    <dbReference type="OrthoDB" id="111287at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13541" checksum="D16592088ED5E138" modified="2011-05-03" version="1">MKTCPIPEEGKPDSEQILRMKEILASLPDDKKLETHSNKLKALSDVTRLKILYLLTDKELCVCEIIYALNKPQSSISHHLNILKNSGFITGRKEGVWIHYRLTDPKITDLVSELIATIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN7</accession>
    <name>F0TAN7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic subunit family 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645460.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1889"/>
    <dbReference type="EnsemblBacteria" id="ADZ10109">
        <property type="protein sequence ID" value="ADZ10109"/>
        <property type="gene ID" value="Metbo_1889"/>
    </dbReference>
    <dbReference type="GeneID" id="10278347"/>
    <dbReference type="KEGG" id="mel:Metbo_1889"/>
    <dbReference type="eggNOG" id="arCOG01803">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_10_2_2"/>
    <dbReference type="OMA" id="YQLKLIP"/>
    <dbReference type="OrthoDB" id="78272at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="2">
        <location>
            <begin position="30"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="316" mass="35125" checksum="CA0F166571A2BD23" modified="2011-05-03" version="1">MKKYLYLGLIVLAVVMVYGTYDYYTVTSDTIVVGYLPSNHHSALFVANAKGMYEKEGFKVQMVPFKNGADMIDAANKNQLDVGFCGITPITSSIDKNSTIKIVAPANEDGSGIVVQNGSNITNLKDLEGKTILEPGSGSIQDVLLRYMLMKNNVSTSNINISQFEVPLMQEALTDNRASAFIAWEPYVTQANLTGEDDVFIYSSDIWDDHPCCVIFATQNMMTKKPDQLRKFLKAHTEATDYINGNLNETSIIVSNKLGTNPNIELASLEHVKFISEPNTEYDNNLMKLVSLQQQLGYVKNNLTLSQIVNYNFLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP7</accession>
    <name>F0TAP7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645470.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10119">
        <property type="protein sequence ID" value="ADZ10119"/>
        <property type="gene ID" value="Metbo_1899"/>
    </dbReference>
    <dbReference type="GeneID" id="43500424"/>
    <dbReference type="KEGG" id="mel:Metbo_1899"/>
    <dbReference type="HOGENOM" id="CLU_2949309_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="59" mass="6996" checksum="3A3C2000BCEA95E7" modified="2011-05-03" version="1">MYDAMQLFSDIDATLAEIRRVIKDCGTLAVMTYVKEGVWKEPEHQEYLEKLDIHFFEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ7</accession>
    <name>F0TAQ7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006598</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0682"/>
    <dbReference type="EnsemblBacteria" id="ADZ08933">
        <property type="protein sequence ID" value="ADZ08933"/>
        <property type="gene ID" value="Metbo_0682"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0682"/>
    <dbReference type="eggNOG" id="arCOG03231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038447_0_0_2"/>
    <dbReference type="OMA" id="TTTICGD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012032">
        <property type="entry name" value="UCP006598"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09890">
        <property type="entry name" value="DUF2117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006598">
        <property type="entry name" value="UCP006598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="376" mass="41222" checksum="CBC215753EB422D4" modified="2011-05-03" version="1">MMKIGVVVHGPQIVDTGYAVKILKLLESYGDVTAILGGTMGRTAVIDAELEDKIDIGQKLLPSQSIEKLSKTSDMVVLINYGKSSVTGHAFGYKVFNRCDADPILLQIERPGEKDGTVIPWNKDLTAFAENIARDLKLQITDPSLITDKMELNGVGAFKSGNIIHRKVAGVSPDENILLNGIVVGRSTSENVILVAEDGILTQILGGIIKNHGVEKLGKIDLENAVVKTGLLRRSKVKPRIIKSKQNAKNRNTSKLSISYLSHAAEDIYKLKDSDLVVTVGDDTTLVAADILYRFNIPIIGITDGDLDRVVEEGFKPVGSMIIELDSGWDDIIGEKIFLELFNGKQTMEIENIENFKSKIIQIISNTTSKYNLKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS1</accession>
    <name>F0TAS1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfate-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0696</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644298.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0696"/>
    <dbReference type="EnsemblBacteria" id="ADZ08947">
        <property type="protein sequence ID" value="ADZ08947"/>
        <property type="gene ID" value="Metbo_0696"/>
    </dbReference>
    <dbReference type="GeneID" id="10277141"/>
    <dbReference type="KEGG" id="mel:Metbo_0696"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="ILMMRAV"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="312" mass="34943" checksum="A77497CAAE8DBD92" modified="2011-05-03" version="1">MDYMIEAIDLTKKFDDLTAVDGLNIKIRKGEVFGFLGPNGAGKTTSIRMMVGLLKPTSGTILIDGTDIKQIEKGKIGICPQELMLWEHLTCKESLSLMADMFEVPKDVKDQRIHELLKDLFLTDKANTVVSNLSGGMKRRLNLALSVVHEPDVVVLDEPSEGLDPQSRRVLWNYIRSMRDVQGKTVILTTHLMDEADSLSDRIGIVDHGKLIRLDTPENLKKEIGEGDIVDVKLSDPSKNLELIKEFKEIQDIISVVETDGRINIRALDAVSKLPQIMKLVEKLDLRVDDLSVRQNTLEDVFIDLTGTGLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT0</accession>
    <name>F0TAT0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphoglycerate kinase</fullName>
            <shortName evidence="3">2PGK</shortName>
            <ecNumber evidence="3">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pgk2</name>
        <name evidence="6" type="ordered locus">Metbo_0705</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0705"/>
    <dbReference type="EnsemblBacteria" id="ADZ08956">
        <property type="protein sequence ID" value="ADZ08956"/>
        <property type="gene ID" value="Metbo_0705"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0705"/>
    <dbReference type="eggNOG" id="arCOG01967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848909_0_0_2"/>
    <dbReference type="OMA" id="EMPFSKG"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-cone" evidence="5">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="303" mass="34544" checksum="6C940272936F14F9" modified="2011-05-03" version="1">MKKIMVEGQVGGKVYREPFSKGVLSRSLTRAEMDPDRAYTFASKIESDLMEQGVKVINLDDLIVIVREKLRGEGGEIAEKYGLWKRIRRCDEPLIILIGGSSGVGTSSIAFEVANRLGIRNMISTDMIREVMRKIVSKELLPTIYESSYTAYRSLRIPPPPELDEVLIGFRDHVDTVSVGIEAVIERSLKEGISIVIEGVHIVPGFISEDLVKKHNVNMFVLTLQDEEVHKGRFYSRCRQQWARRPLKRYMNYFGAIRRTHKYFESQANKYHVPVIENIDVTTTIDSIIETITKTYGSDDDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV6</accession>
    <name>F0TAV6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644333.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08982">
        <property type="protein sequence ID" value="ADZ08982"/>
        <property type="gene ID" value="Metbo_0731"/>
    </dbReference>
    <dbReference type="GeneID" id="10277178"/>
    <dbReference type="KEGG" id="mel:Metbo_0731"/>
    <dbReference type="eggNOG" id="arCOG09726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197250_1_0_2"/>
    <dbReference type="OMA" id="IIYRAYF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="57" mass="6018" checksum="D0BFFE1E7A8C3DB1" modified="2011-05-03" version="1">MDILKDEGGQGAAEYILLFGGVIVIAVAALIIYRAYFSQNSGLNASQDVNGVRSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX4</accession>
    <name>F0TAX4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0749</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644351.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0749"/>
    <dbReference type="EnsemblBacteria" id="ADZ09000">
        <property type="protein sequence ID" value="ADZ09000"/>
        <property type="gene ID" value="Metbo_0749"/>
    </dbReference>
    <dbReference type="GeneID" id="10277196"/>
    <dbReference type="KEGG" id="mel:Metbo_0749"/>
    <dbReference type="eggNOG" id="arCOG01475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_591369_0_0_2"/>
    <dbReference type="OMA" id="SVWAREN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="462" mass="52933" checksum="C875BEB8054B9F9F" modified="2011-05-03" version="1">MSKKEYKPLGYEKFVIMAIFERGSISLKDLEDITIVFISSIWYNVHYGKQYNILGRILLYLFHLRDRTWERYYAYRFKEKHVVKHGLRTVDTCNKLVEVGILKINDEKYELTETGLKLAKLNQERFEKRAEMIENRLFAPSGAAINTTVVDGFMAFLKLSVGFITGSVGIISDGTDSATDTISAFLVWVGIKTKHESLSTLLVIVLLFVASITVMYESFSRLYTAWTSTLSPIIDPSLVIIIEIFAVFVYAGLFFYQRQIGRASGSLTLISQSVDSKNHIFIASSVAIGAGFSFFGIYYVDAIVGAVVAFRIFTDAIGLLKDAIHSIKGEETDLHKYKTPVENYVQDGKLKAFRIFTIFSVWARENEEKENIISSLETMYTQTYIPVLSELHLIPEDEYGFKTDFDKIMDPLVKRNFILVQNGKYTITKSGIKRFKHFVALFKYYDVRLTDLFMLDIEHEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB26</accession>
    <name>F0TB26_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ordered locus">Metbo_1952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645523.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1952"/>
    <dbReference type="EnsemblBacteria" id="ADZ10172">
        <property type="protein sequence ID" value="ADZ10172"/>
        <property type="gene ID" value="Metbo_1952"/>
    </dbReference>
    <dbReference type="GeneID" id="10278412"/>
    <dbReference type="KEGG" id="mel:Metbo_1952"/>
    <dbReference type="eggNOG" id="arCOG04449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="OMA" id="FLYGMVR"/>
    <dbReference type="OrthoDB" id="62389at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="130"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="271" mass="30901" checksum="F9D86C9B6FA2E7E5" modified="2011-05-03" version="1">MLKIAFKVAYIGTDYHGFQRQPNLPTIEGELLRAFKKSGISDSPEKSDYSIAGRTDRGVSALGNVVCLKTDKDVTINRINYYLPSNIRIIGSTMVSNGFKPRYAKLRHYRYILDKSLCPNTLNLKLMKQAAKQMEGKHNYINFSKRSERAPERMVYSVDLVETPDNIVFDVVGESFLWNMVRKMVKVLVMFGREELTLENLETLFNPDVPAAIKPVASDGLILMDVVYDGIEFPCEGYAVNNFMKTLKQEYARKKTILDVEKEMINVLNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB53</accession>
    <name>F0TB53_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Xenobiotic-transporting ATPase</fullName>
            <ecNumber evidence="10">3.6.3.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1979</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.44" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1979"/>
    <dbReference type="EnsemblBacteria" id="ADZ10199">
        <property type="protein sequence ID" value="ADZ10199"/>
        <property type="gene ID" value="Metbo_1979"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1979"/>
    <dbReference type="eggNOG" id="arCOG02841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_2"/>
    <dbReference type="OMA" id="ERQRMTI"/>
    <dbReference type="OrthoDB" id="7900at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="335"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ10199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="559" mass="62686" checksum="175D8522AAC6452D" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB77</accession>
    <name>F0TB77_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl2</name>
        <name evidence="7" type="ordered locus">Metbo_0777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644379.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0777"/>
    <dbReference type="EnsemblBacteria" id="ADZ09028">
        <property type="protein sequence ID" value="ADZ09028"/>
        <property type="gene ID" value="Metbo_0777"/>
    </dbReference>
    <dbReference type="GeneID" id="10277225"/>
    <dbReference type="KEGG" id="mel:Metbo_0777"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_3_2"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="74812at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_L2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_L2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="6">
        <location>
            <begin position="92"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="25855" checksum="7BD20281649F9DB3" modified="2011-05-03" version="1">MGKRLIIQRRGRGTPTYKSASHRFRGKISYRSYDDVEKEGSLKGKVVDIIHDPGRTAPVALVKFENGEENLILAPEGIQIADQVECGISAPINPGNSLPLGEIPEGTPLYNLEKHPGDGGRFVRSSGTYASLITHDVGKAIVELPSGELKAFNPRCRATVGVVAGGGRKEKPYLKAGNRYYALKAKGKKNVSVRGVAMNAVDHPHGGGNRQHPGRPTTVSRHAPAGRKVGSIAASRTGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB7</accession>
    <name>F0TBB7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MEMO1 family protein Metbo_0817</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644419.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0817"/>
    <dbReference type="EnsemblBacteria" id="ADZ09068">
        <property type="protein sequence ID" value="ADZ09068"/>
        <property type="gene ID" value="Metbo_0817"/>
    </dbReference>
    <dbReference type="GeneID" id="10277266"/>
    <dbReference type="KEGG" id="mel:Metbo_0817"/>
    <dbReference type="eggNOG" id="arCOG01728">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="OrthoDB" id="82745at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PTHR11060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="30758" checksum="01C5FB16D1992F41" modified="2011-05-03" version="1">MIRKPAVAGIFYEKDPDMLRKQIEWCFKHNLGPGSIPDIGNERTIKGVVTPHAGYIYSGPVAAHSYHDIAEDGFPETFIVLCPNHTGLGSGVSTMNQGSWETPLGNVEIDETFADLLIEKTGIMDSNPDAHLREHSAEVQLPFLQYLDPDFKFVPVTMWMQDLQTSLEIGVSIAQAAKELDRDVIVIASTDFTHYQPKKQAYMQDMQVIEAIKAMDEKRMMNVVAEQNVTMCGYGPVAATLVAVKQMGANSSELKKYATSGDTTGDNSSVVAYASMVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC1</accession>
    <name>F0TBC1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease J</fullName>
            <shortName evidence="8">RNase J</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnj</name>
        <name evidence="10" type="ordered locus">Metbo_0821</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644423.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0821"/>
    <dbReference type="EnsemblBacteria" id="ADZ09072">
        <property type="protein sequence ID" value="ADZ09072"/>
        <property type="gene ID" value="Metbo_0821"/>
    </dbReference>
    <dbReference type="GeneID" id="10277270"/>
    <dbReference type="KEGG" id="mel:Metbo_0821"/>
    <dbReference type="eggNOG" id="arCOG00546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_4_1_2"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="OrthoDB" id="12384at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="15"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="384"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="49876" checksum="F1C6EDE0CCED5AD1" modified="2011-05-03" version="1">MSVEVIAIGGYEEVGKNMSAVKVGEDVIIFDMGIHLDRIHIHEDTDIARMHSLDLIERGIIPDDTLMKDVDGKVRAIVFSHGHLDHIGAVAKLAHRYEAPLIATPYTMALIEKTIKGERKFKVTNPLKVLNSGEKMQISPDITLEFVRTTHSIPQTVTPALHTSEGIIVYSNDFKFDNHQTLSPPPDYQRFRELGRKGVLAAIIDTTRAAEDDQLKTHSEKVARLVLRDIMEEPLKEDSGMIITTFSSHIERIQAITNIASESKRKILLLGRSMERFCTMAENMGILKLPKSASIYGSPKAVNRALARADENRSDYVLVTTGHQGEPDALLPRIANGKTMFNVKPGDNVVISASVIPNPMNIANRNLMERRLKSSGARIFTNAHVSGHAGKEDHRDFIRMLDPKHLIPSHGNLNMLAAYTELAEEEGYKLGNDIHILRNGQAQVFNGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC6</accession>
    <name>F0TBC6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644428.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0826"/>
    <dbReference type="EnsemblBacteria" id="ADZ09077">
        <property type="protein sequence ID" value="ADZ09077"/>
        <property type="gene ID" value="Metbo_0826"/>
    </dbReference>
    <dbReference type="GeneID" id="10277275"/>
    <dbReference type="KEGG" id="mel:Metbo_0826"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_1_4_2"/>
    <dbReference type="OMA" id="CIFSMNE"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="3"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="28070" checksum="A32AF81B494DDF7C" modified="2011-05-03" version="1">MSEVIGIINQKGGVAKTTTAINLAATLNQKGKKVLLVDVDPQANATTGLGIDKTNLEFSIRDVLLEECEIQDAIISTDYEGLDVLPSNLGLSKLEKQLAGETAPEYILRRYLETVYDDYDMIIIDSPPTLGRLAYNVLVASDSVIIPVQTEYYAMEGVVDLLDAIKEVEEKLYSETEIKGVLLTMHDKREKLTNEVAALVQEYFKDQMFKTIIPRNAPVKRSAADGVPCVIKYPESTGAIAYLKFTDEFLERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBE5</accession>
    <name>F0TBE5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10214">
        <property type="protein sequence ID" value="ADZ10214"/>
        <property type="gene ID" value="Metbo_1994"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1994"/>
    <dbReference type="eggNOG" id="arCOG06499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335060_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="205" mass="22584" checksum="8C9C9D789707E35F" modified="2011-05-03" version="1">MVTNDEIRQRLHEQREGTNIKEYLVCNNCEGAYELLPGEKPEDFSDECECGGKLTYSKDSSINDNKPTKTNKMVKNGLIIFIVVLGLVIFIGPMAYIGFVGMVVSHDEESGSAYDQLAVLKSDYNSLNDQFTNVQTSVDSSNNQNAKTAFINAKLELVKANSDINDVESALSSGKSKDEITQRVNTAQTQLNKAQSELSNVKAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG7</accession>
    <name>F0TBG7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645587.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2017"/>
    <dbReference type="EnsemblBacteria" id="ADZ10236">
        <property type="protein sequence ID" value="ADZ10236"/>
        <property type="gene ID" value="Metbo_2017"/>
    </dbReference>
    <dbReference type="GeneID" id="10278477"/>
    <dbReference type="KEGG" id="mel:Metbo_2017"/>
    <dbReference type="eggNOG" id="arCOG10259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271081_0_0_2"/>
    <dbReference type="OMA" id="QLLYWHV"/>
    <dbReference type="OrthoDB" id="121695at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="12177" checksum="5BF9DFF1FC75FC57" modified="2011-05-03" version="1">MGYETFIPSIPVIIGYLVTYTCYKKNLIKKRVHISIWNLAILLTFLVSGLGGFFLVILMDLGLTSPVNGQLLYWHVEFGITMILVGLFHIHTYWNSTKKMLNLNVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBG8</accession>
    <name>F0TBG8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2018"/>
    <dbReference type="EnsemblBacteria" id="ADZ10237">
        <property type="protein sequence ID" value="ADZ10237"/>
        <property type="gene ID" value="Metbo_2018"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2018"/>
    <dbReference type="eggNOG" id="arCOG05048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173390_0_0_2"/>
    <dbReference type="OMA" id="NDPYPGQ"/>
    <dbReference type="OrthoDB" id="91029at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="228" mass="24740" checksum="2147E8F55D80C15D" modified="2011-05-03" version="1" precursor="true">MKQQLYNILKNKKVVNAALTVPAIAVATMSGSCAAGCPYGLVNDPYPGQCPRYTDLNGDGICDLSQTITSTTTNSGTTDSSSSTNNSSDDSNSTVNLDSNNGGDGTTFSDGADFNVIPLSLIIIGIYLFTHFLFSRGILSQKKHRRIWNLFLTAGFVGTGFTGALLLLLINLGIKTALNPSIDYWHAEISILMVIAVLIHMHIYRKPLKRMFKIFGSMQKPKKVKPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH4</accession>
    <name>F0TBH4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2024</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645594.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2024"/>
    <dbReference type="EnsemblBacteria" id="ADZ10243">
        <property type="protein sequence ID" value="ADZ10243"/>
        <property type="gene ID" value="Metbo_2024"/>
    </dbReference>
    <dbReference type="GeneID" id="10278485"/>
    <dbReference type="KEGG" id="mel:Metbo_2024"/>
    <dbReference type="eggNOG" id="arCOG00945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_10_0_2"/>
    <dbReference type="OMA" id="YPCYRFV"/>
    <dbReference type="OrthoDB" id="18533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024018">
        <property type="entry name" value="CHP04083_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04083">
        <property type="entry name" value="rSAM_pep_methan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="378" mass="42934" checksum="BB78D44F80B4F3B6" modified="2011-05-03" version="1">MVFHVMLVPTLGCPSNCGYCWSSEEGSELMSIDTLKQTVNWLKTLREDSVTVTFHGGEPLLAGLEFFQTALPLIKTELDHLKPALAIQTNLWNMTDDLAELFSTYNIPIGSSIDGPEELNDYQRGEGYYQKTMQGYKIAKAHNLKVSFICTFTSHSIKHKEEILNYFLENDLTLKLHPSLPSMRDDNPDEWALSPEDYGELLVYLLEEYLDKMDEVEIMNIDNLAKSVFRRRGTVCTFVDCMGDTFAIGPDGSIYPCYRFVGMPDYVMGNVHDDPSLDDLAESKPWQLLMEFKDFVDTDCKRCNYVKFCRGGCPYNALAITDAKVEAVDPHCTAYKRIFKEITDKATKEMLSGSLMGNSKPSKTTKYSIMDLMLKESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI7</accession>
    <name>F0TBI7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="2">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645607.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2037"/>
    <dbReference type="EnsemblBacteria" id="ADZ10256">
        <property type="protein sequence ID" value="ADZ10256"/>
        <property type="gene ID" value="Metbo_2037"/>
    </dbReference>
    <dbReference type="GeneID" id="10278498"/>
    <dbReference type="KEGG" id="mel:Metbo_2037"/>
    <dbReference type="eggNOG" id="arCOG00619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023342_2_0_2"/>
    <dbReference type="OMA" id="FVSDMSF"/>
    <dbReference type="OrthoDB" id="14505at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020075">
        <property type="entry name" value="Uncharacterised_AF2234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10967">
        <property type="entry name" value="DUF2769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_synthase" evidence="3">
        <location>
            <begin position="115"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="499" mass="54030" checksum="B8D63678714E9AFB" modified="2011-05-03" version="1">MVQDSESKLMKIKNSPENRAKCHCKLCPSYPYKCGGEVLYCGKGPSKCDVDIEVCICDTCPIYFEYNLKGIYFCDKDMVGENKVFMRKKRSDEDDDRYQAVVDIKDESAVGESVVGSMGSLKKLPFSLDDLYFVPAQVMILPLNATDPVKTSIVLGKDAKKPLKLSSPIMISGLSFGAVSKSAKIVISKTASNLNVGFNSGEGGVLDEELESSKTMVVQYSTGRFGVEDEILKNAAAIEIRFGQGAYPGKGSYLPAEKMTEEVSSKRNLENGEPAYSPAHHPDILTPRDLKKKVSKLRRMSSGAPIGAKIGCGNVEDDVKVLVEAGVDFIALDGFGGGTGATDKYVRENVGIPIFSALPRAKQTLENLKPKRRVSLIGSGGLRSSADFAKCLALGADAVYIGTAALIAINCEQYRLCYTGNCPTGIATQNPKLEKQVDQEESVHKLINFIKLSSQEVANLTRITGKDNVSKLDKSDLVSVNRDLAVITGVKWINGELQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBS3</accession>
    <name>F0TBS3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Teichoic-acid-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0901</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.40" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0901"/>
    <dbReference type="EnsemblBacteria" id="ADZ09150">
        <property type="protein sequence ID" value="ADZ09150"/>
        <property type="gene ID" value="Metbo_0901"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0901"/>
    <dbReference type="eggNOG" id="arCOG00210">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03220">
        <property type="entry name" value="ABC_KpsT_Wzt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015860">
        <property type="entry name" value="ABC_transpr_TagH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="20"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="62"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="39454" checksum="D5EFCB1E10B5622E" modified="2011-05-03" version="1">MGEIVIKVEDVGMEYNLNKEKVDNLKEFVIKFLKRELKFTKFWAVRHVSMEVERGDKYGIIGLNGAGKSTLLKVISGVIKPTEGDVSIKGSIVPLLELGSGFDPEYTGRENIFLKGSLLGYSREYLESKYDEIVEFSELEEFIDVKLKNYSTGMSSRLAFSIATAVNPQIMILDEVLSVGDAKFREKSLERMKTLLDQDVSVLFVSHTLQQVRDLCNKAIWLENGRVIMQGLVDEVCDSYERSVLLKEKIYVKSSNPKKNDAKVPVDKDIILTFNAPIKVGTNWIELLSANRVSVPLEKYVDGDKLIIKHPVLQPGMRYNLHLHTGCFEDLEGNKVNTHSTGFTTEIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBW4</accession>
    <name>F0TBW4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2087"/>
    <dbReference type="EnsemblBacteria" id="ADZ10306">
        <property type="protein sequence ID" value="ADZ10306"/>
        <property type="gene ID" value="Metbo_2087"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2087"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033995_0_0_2"/>
    <dbReference type="OMA" id="WAVAFAC"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="3">
        <location>
            <begin position="15"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="413"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="501"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="559" mass="60975" checksum="4523363EDB03EA68" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX5</accession>
    <name>F0TBX5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="6">ATase</shortName>
            <ecNumber evidence="6">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="6">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purF</name>
        <name evidence="12" type="ordered locus">Metbo_2098</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6 9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6 10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645668.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2098"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="ADZ10317">
        <property type="protein sequence ID" value="ADZ10317"/>
        <property type="gene ID" value="Metbo_2098"/>
    </dbReference>
    <dbReference type="GeneID" id="10278561"/>
    <dbReference type="KEGG" id="mel:Metbo_2098"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="37299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 7 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="6 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="5"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 8">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="466" mass="51281" checksum="B53C47FA10F30DF2" modified="2011-05-03" version="1">MRDKCGIVGAYSRDKSVHVSRQIYYGLYSLQHRGQESAGISAHDGNQLSTYRGMGLVCDVFNNGNVEGIDGNVGIGHVRYSTTGKSKIENSQPMFSNFELGTVAVAHNGDIINSPELRCELELLGYEFESTTDSEVLCHLLTREILKTSNVVESIRNVTKMLNGSYSLVILFNEDLYVVRDPNGIKPLSMGELGDLTMVASETVAFDVLGAKHLRDVEPGEIIRIGDKIESYKLSETEKPRRAHCMFEYVYFARPDSVLDGSSVYNVRLNIGKALSKEFPADADVVMPVPDSAITAAIGFSRESGLGYGEGLLKNRYVGRTFIMPTQVERETSVRLKMNPIRSELEGKSIVLVDDSIVRGTTSKSLVKVLREAGAKEIHLRVGCPPIISPCYYGIAMATKKELIASDKDVEDIRKTLGVDSLGYLSLESLIECIGIEDGNLCVGCLTGEYPTELPKDIKEYEAKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC54</accession>
    <name>F0TC54_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644556.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09205">
        <property type="protein sequence ID" value="ADZ09205"/>
        <property type="gene ID" value="Metbo_0958"/>
    </dbReference>
    <dbReference type="GeneID" id="10277407"/>
    <dbReference type="KEGG" id="mel:Metbo_0958"/>
    <dbReference type="eggNOG" id="arCOG06457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163810_0_0_2"/>
    <dbReference type="OMA" id="MNEGNIT"/>
    <dbReference type="OrthoDB" id="80359at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025101">
        <property type="entry name" value="DUF4012"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13196">
        <property type="entry name" value="DUF4012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="238" mass="25424" checksum="72D9FA4074006AF8" modified="2011-05-03" version="1" precursor="true">MRKIIKILIVIILILLASVAAFVIYQYEQPSSTNVMKGDHNILLLTADPSEKRPGVGGVDMAFVIHVTDGDIKNLTPVYPGGMTHPTAQEPAAANAGNGKLRFHDVLWDANITQDTVLAQEIVKYNTNMTTDAVVIVTPDAVDAVLNSIGPINIPGYNGNGTNAAVAYIRNESEFSSNMSRGNATETLMKPVMAAAKDPSKAPALFSTIISQYSKGNIIVVPSDLMTQFAISKGLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCA6</accession>
    <name>F0TCA6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10373">
        <property type="protein sequence ID" value="ADZ10373"/>
        <property type="gene ID" value="Metbo_2158"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2158"/>
    <dbReference type="HOGENOM" id="CLU_1912358_0_0_2"/>
    <dbReference type="OrthoDB" id="140549at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14034" checksum="0B1ACDF3887CD288" modified="2011-05-03" version="1" precursor="true">MEKLTKILIVVVIILVAGLSLTVGLLLGTSFNKPVITNNTTNSSNSSVQNQTNSTNQTKVDSTKSKYISKAEAIRLAKAAWPVPEATYSISSYPTDDSPYYWVDVLNEPATIGPGGFVKINAITGKIISAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCD3</accession>
    <name>F0TCD3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="5">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rrp41</name>
        <name evidence="8" type="ordered locus">Metbo_2185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645751.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2185"/>
    <dbReference type="EnsemblBacteria" id="ADZ10400">
        <property type="protein sequence ID" value="ADZ10400"/>
        <property type="gene ID" value="Metbo_2185"/>
    </dbReference>
    <dbReference type="GeneID" id="10278648"/>
    <dbReference type="KEGG" id="mel:Metbo_2185"/>
    <dbReference type="eggNOG" id="arCOG01575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_0_0_2"/>
    <dbReference type="OMA" id="KGKGQGW"/>
    <dbReference type="OrthoDB" id="57069at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0271">Exosome</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase_PH" evidence="6">
        <location>
            <begin position="27"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="RNase_PH_C" evidence="7">
        <location>
            <begin position="159"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="26392" checksum="10C1A301559D3142" modified="2011-05-03" version="1">MIIIITNTNLSTGTETSGRADNRAFDELRKMKIEAGVLERADGSAYLEIGGNKVLAAVYGPRELFVRRLMQPNKAVLRCKYNMAPFSVDDRKRPGPDRRSVEISKLASQALTPAVFLEKFPRSTIDVFIEVIEAEGGTRCAGITAASVALADAGIPMRDMVVACAAGKSDGKVILDLSEVEDKEGQADVPIAIMPRTGEITLLQMDGNLTEEEFEQALDLAINGCKIISEKQKAAIKNRYGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCF4</accession>
    <name>F0TCF4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cobQ</name>
        <name evidence="6" type="ordered locus">Metbo_2207</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2207"/>
    <dbReference type="EnsemblBacteria" id="ADZ10421">
        <property type="protein sequence ID" value="ADZ10421"/>
        <property type="gene ID" value="Metbo_2207"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2207"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="OMA" id="EIHHGVA"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="259"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="510" mass="56142" checksum="6EC4FDFC7FC4625A" modified="2011-05-03" version="1">MQRENNYPCIMIQGTSSNAGKSVAVTALCRIFAKRGYKVAPFKSQNMSLNSYTTNEDAEIAMAQVLQAEAAGVEPSYNMNPILLKPKEDFISQVIVHGKPAGDMNFYHYQNNFRQEALAAIESSINALKEDYDVIVIEGAGSPAEINMLDKDLANMQIARMADADVILVADIDRGGVFASIAGTFSLLPEEDRNRIKGIIINKFRGNLDILMPGIRQIEEIVGVPVLGVLPYDDDLKLPEEDSASLSEHKYRGDGDVVIGVLRLPRISNFTDIDPLEYEPDVTIKLIEMDDEIGDVDALILPGTRNSVSDLVALKKSGHADKIVELSKKIPVFGICGGYQILGTMIHDEDHQESKHGTVEGLGLLDIETRFGEVEKVITQSNGILTGKGMFKDLEGELVEGYELHEGTSSINNSQALIKIVKGFGNDSKGYDGAVNGLVSGTYLHGIFHNFKFRRTFTDHVRSSKGMEKLGFGTDDFEDMKRSSIERLANIFEENVDMSIIDAKLVDIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH2</accession>
    <name>F0TCH2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644600.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1002"/>
    <dbReference type="EnsemblBacteria" id="ADZ09249">
        <property type="protein sequence ID" value="ADZ09249"/>
        <property type="gene ID" value="Metbo_1002"/>
    </dbReference>
    <dbReference type="GeneID" id="10277451"/>
    <dbReference type="KEGG" id="mel:Metbo_1002"/>
    <dbReference type="eggNOG" id="arCOG09731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014010_0_0_2"/>
    <dbReference type="OMA" id="ADIMVNS"/>
    <dbReference type="OrthoDB" id="4105at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007165">
        <property type="entry name" value="Phage_holin_4_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04020">
        <property type="entry name" value="Phage_holin_4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="688" mass="77013" checksum="28869C6FC7885139" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCI4</accession>
    <name>F0TCI4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proline--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="7">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proS</name>
        <name evidence="9" type="ordered locus">Metbo_1014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644612.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1014"/>
    <dbReference type="EnsemblBacteria" id="ADZ09261">
        <property type="protein sequence ID" value="ADZ09261"/>
        <property type="gene ID" value="Metbo_1014"/>
    </dbReference>
    <dbReference type="GeneID" id="10277463"/>
    <dbReference type="KEGG" id="mel:Metbo_1014"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="OrthoDB" id="17290at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PTHR43382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="SSF64586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="36"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="469" mass="54329" checksum="110E43A98241C727" modified="2011-05-03" version="1">MSEFSEWFHNILEEAEIIDTRYPVKGMHVWLPQGFKIRKHTLEILKTILDKDHEEVMFPLLIPEDELAKEAIHVKGFEEEVYWVTHGGLTELNKKLALRPTSETAMYPMFSLWVRNHSDLPMRYYQVVNTFRYETKHTRPLIRVREITTFKEAHTVHSSAEETEEQVKEALRIYRQFFDSLGIPYTVSKRPEWDKFPGALYTMAFDTLLPDGKTLQIGTVHNLGQTFAKTFDITYETAEGNHEYVYQTCYGLSDRVIASLIGIHGDDSGLCLPPDVAPYHVVIVPIIFKKGGEVVIDFCKKLQEKLESELNLRVYFDDRDIRAGKKYYEWEMRGIPLRLEVGPRDIENHKIVTFRRESKEKEIIDYDDTTISKTVNNLLDNISTKMREKAWKSLEDHVHHVETLEEASTTISEDGGIVTFDWCGDQECGKEIEEKVNVDILGVQADDENGICINCGKKSKHITLIAKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK0</accession>
    <name>F0TCK0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron (Metal) dependent repressor, DtxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644628.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1030"/>
    <dbReference type="EnsemblBacteria" id="ADZ09277">
        <property type="protein sequence ID" value="ADZ09277"/>
        <property type="gene ID" value="Metbo_1030"/>
    </dbReference>
    <dbReference type="GeneID" id="10277479"/>
    <dbReference type="KEGG" id="mel:Metbo_1030"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_1_2"/>
    <dbReference type="OMA" id="PKYDPHG"/>
    <dbReference type="OrthoDB" id="110491at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="241" mass="27408" checksum="129A9A1026D3CCCD" modified="2011-05-03" version="1">MSENKKLSANIEEYLETIYKLSQKDEDVKTSKISKDLGITQASVSEMLKKLDKLEYVKYSQYKGVRLTDKGLKTAQKVTRKHRLLERFLHDILKLKDNLLHDQACEMEHSLSDEAERAMCQVLEYPDKCPGDSVIPACDLKFTSCDECMNRREEEVNEVGKRNENLIPIMDLKDHQKGKVSFIRGDYKVIRRLLDMGITIGAFISVVKIAPLSGPVEVAIRGSKLAIGRDIACNVFVELVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM4</accession>
    <name>F0TCM4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1054"/>
    <dbReference type="EnsemblBacteria" id="ADZ09301">
        <property type="protein sequence ID" value="ADZ09301"/>
        <property type="gene ID" value="Metbo_1054"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1054"/>
    <dbReference type="eggNOG" id="arCOG00940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="OMA" id="IVWNFTN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034391">
        <property type="entry name" value="Cmo-like_SPASM_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01387">
        <property type="entry name" value="BtrN-like_SPASM_domain_contain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="123"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="506" mass="57052" checksum="BC7B559243490611" modified="2011-05-03" version="1">MENCAKEKKSDMDIGDMIRSFEAVANNPFSKFIISRTFDYCEKDHGNRLEVGLELLFNKRKTACIRCKGLSKMLSFIINKGVKTFGVTDDDLKELMEDPYWIKGLSSVIKGIGEFGVKKPFVPGAPFQVVWNITKSCNMNCAHCYENAGKKADDELNSEQIKQCLETLSRSGVTSIAFSGGEPTTKPNIRNHIKDVADLGIYPAMATNGYSLSNKKLCDKYVDSGLQFVQISLDGLNPETHDKFRGIDGAWERAVQAIKNFVEEDIFVEVSTTVTQHNIDEIPSMINFVRELGAHWLMLYNFIPTGNGLNISEMDISPQKRFNMLKTAYNENFKGDMQVLSTAPQYASVAESLQNMKSSIIPTHFYNPEYENPQIMQLAEFVGGCGAGRFYMSLEPNGDMYPCVFFPHDEFLRLGNILEDDFEEIWQNQPLLNDLRNKTLLEENCGSCDNKNICGGCRARAYTYLDNVQAPDPGCINNQVKWDGIRTKTPENISNTEDKYVNSECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN0</accession>
    <name>F0TCN0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644658.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1060"/>
    <dbReference type="EnsemblBacteria" id="ADZ09307">
        <property type="protein sequence ID" value="ADZ09307"/>
        <property type="gene ID" value="Metbo_1060"/>
    </dbReference>
    <dbReference type="GeneID" id="10277509"/>
    <dbReference type="KEGG" id="mel:Metbo_1060"/>
    <dbReference type="eggNOG" id="arCOG00051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080337_0_0_2"/>
    <dbReference type="OMA" id="AFIMACI"/>
    <dbReference type="OrthoDB" id="95382at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="326" mass="33857" checksum="2EB51BC7874BF58E" modified="2011-05-03" version="1">MVDVKEIKSIELVPFTLMTSSISAILGLIYAILLLIAFGALSAFLPGAALIFASLGISMIILYPLGTFLVYIVLSFLTALLYNTFVPRLGGIKLGLEGDQITSLPVVSFALIQSAVAAAWALIVGLLLTAIILPLTTLISTVIPLVMQLGVDAINNATAMLSTSTIANNVSINATNFTAANLPNGSAVGNGGVVLALMLIIGLPIAVFIFVFIGTALASIFYNFLIPRVGGVRLLFAQAGSAHEITSIPVVAASLAIAVVALIFGIISGLVGLIGMASAGNAVGGVEFLIYDIIRYFVGTFIMVAIVTIIYNFLAPRIGGIQLKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCN3</accession>
    <name>F0TCN3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09310">
        <property type="protein sequence ID" value="ADZ09310"/>
        <property type="gene ID" value="Metbo_1064"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1064"/>
    <dbReference type="eggNOG" id="arCOG14642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075387_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="5628" checksum="1F366F4CD803FB15" modified="2011-05-03" version="1" precursor="true">MVETNQANGGSLVALAIIFLIILLLFPAFALWLLYVAILVMLITGIISLIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS4</accession>
    <name>F0TCS4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl11</name>
        <name evidence="8" type="ordered locus">Metbo_2250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645815.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2250"/>
    <dbReference type="EnsemblBacteria" id="ADZ10464">
        <property type="protein sequence ID" value="ADZ10464"/>
        <property type="gene ID" value="Metbo_2250"/>
    </dbReference>
    <dbReference type="GeneID" id="10278716"/>
    <dbReference type="KEGG" id="mel:Metbo_2250"/>
    <dbReference type="eggNOG" id="arCOG04372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="98187at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L11_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="6">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ10464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="162" mass="17394" checksum="1478AF3B09FE63BF" modified="2011-05-03" version="1">MAKETVEILIDGGKATPGPPLGPAIGPLGINMMQVVEQINKKTSDFEGMKVPVKVIVDVGTKEFEVTVGTPPTTALIIDELKIEKGSTDPGLEKVADLKVEQALKIARMKFDALLSNDYKNAAKEVIGTCVSMGITVEGKDPRDVQKEIDQGVYDETLVQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS9</accession>
    <name>F0TCS9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2255"/>
    <dbReference type="EnsemblBacteria" id="ADZ10469">
        <property type="protein sequence ID" value="ADZ10469"/>
        <property type="gene ID" value="Metbo_2255"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2255"/>
    <dbReference type="eggNOG" id="arCOG15199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3003190_0_0_2"/>
    <dbReference type="OMA" id="MENKQID"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="58" mass="6547" checksum="9DE5C716CFB01154" modified="2011-05-03" version="1">MEDNQINFILYIMGVVGLIVLLLGVFDFYPIKYGVVGAVIIWIIGGGYRQYYGMGKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT1</accession>
    <name>F0TCT1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645822.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2257"/>
    <dbReference type="EnsemblBacteria" id="ADZ10471">
        <property type="protein sequence ID" value="ADZ10471"/>
        <property type="gene ID" value="Metbo_2257"/>
    </dbReference>
    <dbReference type="GeneID" id="10278723"/>
    <dbReference type="KEGG" id="mel:Metbo_2257"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901947_0_0_2"/>
    <dbReference type="OMA" id="CGMELIV"/>
    <dbReference type="OrthoDB" id="127305at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="2">
        <location>
            <begin position="3"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15165" checksum="2B44056A8678935F" modified="2011-05-03" version="1">MICENCGAEIRNRENYCPNCGMEVISPYNKSIKARYMAGEYTENQGDYYSRKRRSKEEKVYVEETEPQVSKETDPELVEEYYEPEPEDFEESGGVSVLTVIILFLVVALLIGFVIGILVFSGFLHSLPGFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCV0</accession>
    <name>F0TCV0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645841.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2276"/>
    <dbReference type="EnsemblBacteria" id="ADZ10490">
        <property type="protein sequence ID" value="ADZ10490"/>
        <property type="gene ID" value="Metbo_2276"/>
    </dbReference>
    <dbReference type="GeneID" id="10278742"/>
    <dbReference type="KEGG" id="mel:Metbo_2276"/>
    <dbReference type="eggNOG" id="arCOG02078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067052_1_0_2"/>
    <dbReference type="OMA" id="CPAKLTP"/>
    <dbReference type="OrthoDB" id="67615at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002829">
        <property type="entry name" value="DUF116"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43801">
        <property type="entry name" value="PTHR43801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01976">
        <property type="entry name" value="DUF116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006594">
        <property type="entry name" value="UCP006594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="233" mass="26161" checksum="5B1D5EB1889CE847" modified="2011-05-03" version="1">MAFMEFYQIFGQIVFILAIVLLTLLSVTLILGKLLLKEDKLVFPRLLLFTVDMFYGIFKKFSENVGVDGKIVDQIGVEVRNNVNEKVYKHTKPQDKIVVLPHCLRSTECEANLETSGLVCNNCNRCVIGVIKTKAEEKGFKVFIIPGSTFLKKILEKNDFKAVLGVACYQDLNLSMMKLSKFSCQGVPLLKDGCINTKVDVKAVFEKLGEIQNQDPNPISSSCSNESYNQKTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W1</accession>
    <name>F0T5W1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ApeA_NTD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1109"/>
    <dbReference type="EnsemblBacteria" id="ADZ09354">
        <property type="protein sequence ID" value="ADZ09354"/>
        <property type="gene ID" value="Metbo_1109"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1109"/>
    <dbReference type="eggNOG" id="arCOG09518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039923_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041223">
        <property type="entry name" value="ApeA_NTD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18862">
        <property type="entry name" value="ApeA_NTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApeA_NTD1" evidence="1">
        <location>
            <begin position="16"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="477" mass="56719" checksum="EDC42120CF80AF7F" modified="2011-05-03" version="1">MNLGISGVFFMDDKIEFNGYWWFPNKPDEEKVFGKLEYVPGEGAILKLSDFSEDKFEPFDRDPTEPYHEIVLGLTSEGKKITLYKCHNISNSYKGGGHGQEEVFIFTLKVWYIFVGHHFENLSDIKLKCISIQYSNLDEWIDMPLKIDELENRPKSIPLTKMGDYKVFIDFHKSMPFSSFRKMTVTQYANIWIESFQNNKPWAEYENIIRSIQNFLTIAFMHPTYPLKIKGKVRKDKIIQIVDDDFEGPIVESSPFIDIYVTHSQIPKLLEEVNSYQMLFPFDEVKKDIKRYLLNWLVNSSLYNSVYNSYFSTVYNKNLFLENQFLNVIMALEGYHRSRTDNIEIDKYLHNKRVESIIRVAPIEHEKWLESQLNYSNEPSLRARLLNILRPYSDVFRNMTRIESFIYKVVTNRNSLVHKDKNNNIDYGQLFYTTVILKIVFELYLLEDMGFKQEYIKQLIEKRKSHYAFKYMKMVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5W7</accession>
    <name>F0T5W7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SEC-C motif domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644711.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1115"/>
    <dbReference type="EnsemblBacteria" id="ADZ09360">
        <property type="protein sequence ID" value="ADZ09360"/>
        <property type="gene ID" value="Metbo_1115"/>
    </dbReference>
    <dbReference type="GeneID" id="10277565"/>
    <dbReference type="KEGG" id="mel:Metbo_1115"/>
    <dbReference type="eggNOG" id="arCOG06886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051477_0_0_2"/>
    <dbReference type="OrthoDB" id="82506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="483" mass="56197" checksum="AB27536928958963" modified="2011-05-03" version="1">MVNKERVKIGRNDPCHCGSGIKYKRCCLNKDKNLNRSTSNQKNLEENDELNKNKIKDIPSFQKMEDSIKGMEFLKSVTEIGLLDGLIPMGLDVDALKETLSEVDGFVEEYHDITSVPDKFNEAFLKCGWVFYMSMNHEVVTNSIKLYEQRKFNQAENILINYYDENIELFIQWLGWIDEFKPRLELIEKVYEDYANERYHACIPVLLAVIDGIVFDNKETGNKGFFGEENKLTAEDSIAAHITGLPELQKILSSPRTKTTTDELTIPYRNGILHGRDLNYANKSVAIKLWATLFALKDGLVEIKKSKEPKEEEKDLTFSELTASIRKNDMRETLLKKWKPRNLEINKDFPEFSLSSDYLEGSPERTLVEFFELWMKRNFGYMAQKMDHRTLKEYSIGIIAGKFSREIFNKKNLKSFKILEIIDEAPAISEIKTELVIEKKERQLKKEITFRIIYEDEKGQPEIRTMNNGSWKFISCFGEIDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T627</accession>
    <name>F0T627_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2320"/>
    <dbReference type="EnsemblBacteria" id="ADZ10534">
        <property type="protein sequence ID" value="ADZ10534"/>
        <property type="gene ID" value="Metbo_2320"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2320"/>
    <dbReference type="eggNOG" id="arCOG02348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_57_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011495">
        <property type="entry name" value="Sig_transdc_His_kin_sub2_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07568">
        <property type="entry name" value="HisKA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="34"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="167"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="220"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="346"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="420"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="1">
        <location>
            <begin position="484"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="681" mass="78748" checksum="60BF4EF1463C3070" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T639</accession>
    <name>F0T639_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dimethylmenaquinone methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645897.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2333"/>
    <dbReference type="EnsemblBacteria" id="ADZ10546">
        <property type="protein sequence ID" value="ADZ10546"/>
        <property type="gene ID" value="Metbo_2333"/>
    </dbReference>
    <dbReference type="GeneID" id="10278799"/>
    <dbReference type="KEGG" id="mel:Metbo_2333"/>
    <dbReference type="eggNOG" id="arCOG00117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_3_0_2"/>
    <dbReference type="OMA" id="VNVPVVC"/>
    <dbReference type="OrthoDB" id="103576at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="220" mass="23615" checksum="AC0F350A1C3AF587" modified="2011-05-03" version="1">MDGKQKLSPETFLKKFASKSSEEPAYPDVTTSQFSDALTKLTGKNGVLVNIKPIDDFKIIGRATTVKTSSNDWGTVIKGIYSAKKGDVLVISCEDDQTAVWGELASEAAKIRGLSGTVVIGASRDSAGIKSLKYPVFSRNVVPNAGKPLTEGEININLTIEDTIIKPGDLIIGDECGVVSVDQKMVSEVMKVAMKIVSNETEISSKLNQNISFMEILGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T647</accession>
    <name>F0T647_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645905.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2341"/>
    <dbReference type="EnsemblBacteria" id="ADZ10554">
        <property type="protein sequence ID" value="ADZ10554"/>
        <property type="gene ID" value="Metbo_2341"/>
    </dbReference>
    <dbReference type="GeneID" id="10278807"/>
    <dbReference type="KEGG" id="mel:Metbo_2341"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_10_0_2"/>
    <dbReference type="OMA" id="GWHHARV"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="134"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="317" mass="34832" checksum="AEA7929C88290975" modified="2011-05-03" version="1">MNKVNVGVIGVGAMGYNHVRVYSELENANLMAVSDLMRGTLAEVSKEFNTVGFVDYDNILKMPEIDVVSVCVPTTYHYDVVMGAIEQGKHVLVEKPIAFTLDEAKDMVNAAHDAGVKLATGHVERFNPAVDEAKRLLNDGAIGEIVSASAKRVGPFPPRIKDVGVAIDLAIHEVDIMFYLLNSPASSVYANMGSRLEKCEYEDHAEIMMKFENGVVGILETNWLTPYKKRQLEITGVDGIISMDYIDQTVEVYGKNAQKVEVAHTEPLKDELQSFLSCVVNDEEPKITGEDGIHALKVVLAAMKSAQKKIVVNMNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T671</accession>
    <name>F0T671_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645929.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2365"/>
    <dbReference type="EnsemblBacteria" id="ADZ10578">
        <property type="protein sequence ID" value="ADZ10578"/>
        <property type="gene ID" value="Metbo_2365"/>
    </dbReference>
    <dbReference type="GeneID" id="10278831"/>
    <dbReference type="KEGG" id="mel:Metbo_2365"/>
    <dbReference type="eggNOG" id="arCOG00047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057819_1_0_2"/>
    <dbReference type="OMA" id="FFHPGVM"/>
    <dbReference type="OrthoDB" id="47556at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005885">
        <property type="entry name" value="TrmG10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01177">
        <property type="entry name" value="TIGR01177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="49"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="40018" checksum="9E074D729AFBAF71" modified="2011-05-03" version="1">MEIVFIISQEHQTLPRAEIEAVLRAEEIPFNFKKQYEGILIVEVANEHEKYMERVGKRLSYTHELCKLLIETDKTSLNTDVGKVDWNGIITNDYVVRARRMDKTDKFDTSKIEIEIGGTINSIVEGSKVNLKDPDCFIRLIYLDEIVLLTQRIVKIDKKHFNNFKPHKRPFFYPGSMSPKLARCMVNLTEIKEDDTVLDPFCGTGGILIEAGILGAKLIGSDLDYKMVQGTKSNLEYCGFKNYKIFREDAKKIHLETRVNAIVTDPPYGISASTGGEDSTNLYSAALKNMDGLLKEKGKICMATPHYMDIKGLIKDTNFEIIEQHHIRMHKSLTRVISLLTKKNLSQKNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T688</accession>
    <name>F0T688_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1159"/>
    <dbReference type="EnsemblBacteria" id="ADZ09403">
        <property type="protein sequence ID" value="ADZ09403"/>
        <property type="gene ID" value="Metbo_1159"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1159"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_2"/>
    <dbReference type="OMA" id="TTLGLWR"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="12"/>
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    </feature>
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            <end position="159"/>
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            <begin position="14"/>
            <end position="466"/>
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        <source>
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    </evidence>
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            <dbReference type="EMBL" id="ADZ09403.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="484" mass="53192" checksum="86994C9894BD1E7A" modified="2011-05-03" version="1">MNNNYAEKYQINRIMAGLMVGLLIAAFDYSIMSTAMPNIINSLNGIEFYVWPFSAYMLTSTISIIIFGKMSDIYGRKSILVAGIIIFIITSIMCGFSENMIKLIIFRGLQGIGGGILISLPFVMVGEIFSQNERAKYMGILGSVFGIADVLGPILGGVVTDTLGWRYVFFINVPLGIIALILIYNSIPNFIHDHVKKVIDYRGIILFTLGLSSMFLGLTFTGQVTKHPPALIVGLMLMSILMFITFVLVENNADEPIIPLHLFKNSIFNVSSIESFLASAIMFSGIIYIPLFTQGVLGMNAVNSGFIMIPMLLSLTVSSVVTGQIISKTGKYKSLVIIEFLITILGVALLASMDEYIPYYMLILYSTILGIGSGMAYNIFNVAVQNTFSIAEIGIVTASMRFFRNIGTIVFVPVFGFIMNFSLNASTIGSNTTQTLIVAIQNIFYLTLILALLGLIISLFLKETHLCESSSDKTDDDLKYDKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T691</accession>
    <name>F0T691_METLA</name>
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        <recommendedName>
            <fullName evidence="1">UPF0201 protein Metbo_1162</fullName>
        </recommendedName>
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    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
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            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1162"/>
    <dbReference type="EnsemblBacteria" id="ADZ09406">
        <property type="protein sequence ID" value="ADZ09406"/>
        <property type="gene ID" value="Metbo_1162"/>
    </dbReference>
    <dbReference type="GeneID" id="10277612"/>
    <dbReference type="KEGG" id="mel:Metbo_1162"/>
    <dbReference type="eggNOG" id="arCOG01043">
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    </dbReference>
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    <dbReference type="OMA" id="VHKQVAY"/>
    <dbReference type="OrthoDB" id="106305at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
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        <property type="entry name" value="UPF0201"/>
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        <property type="entry name" value="PTHR39652"/>
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        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="129" mass="14621" checksum="1EC2A638F99F3225" modified="2011-05-03" version="1">MNCKVTVRAKVNPTEELDKVIRAISNIFDYEELEIGDGYVEVTGDENSVLKLKEELKNRKIRDTANKMLFKGIENNKISFSLGKQAALVSVPNFVDSKMSALGELDVEIETDNPEVFIAWITHKEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A7</accession>
    <name>F0T6A7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1178</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644773.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1178"/>
    <dbReference type="EnsemblBacteria" id="ADZ09422">
        <property type="protein sequence ID" value="ADZ09422"/>
        <property type="gene ID" value="Metbo_1178"/>
    </dbReference>
    <dbReference type="GeneID" id="10277628"/>
    <dbReference type="KEGG" id="mel:Metbo_1178"/>
    <dbReference type="eggNOG" id="arCOG01847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_10_0_2"/>
    <dbReference type="OMA" id="GDDVMLY"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SATase_N" evidence="6">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="237" mass="25821" checksum="AF9520B46D61CA6A" modified="2011-05-03" version="1">MFDRIREDIQMVFSRDPAARSTAEVILFSPGLHAIWFHRLGNWFWTRNHLFWGRFISTLNRFLTGVEIHPGATIGRRVLIDHGMGVVIGETAEVGEDVLIYQGVVLGGTSLEKKKRHPTVGNGVVIGSGAKIIGNIKIGDCCKIGAGSVVLKPTPPGATVVGIPGRVVQESRKCAIDLDHGQLPDPVAEVIQLILQRQDELEAEIKQLGISTDIVKINGFLTKKSEIEEIFSEGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6C5</accession>
    <name>F0T6C5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644791.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1196"/>
    <dbReference type="EnsemblBacteria" id="ADZ09440">
        <property type="protein sequence ID" value="ADZ09440"/>
        <property type="gene ID" value="Metbo_1196"/>
    </dbReference>
    <dbReference type="GeneID" id="10277646"/>
    <dbReference type="KEGG" id="mel:Metbo_1196"/>
    <dbReference type="eggNOG" id="arCOG01864">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_44_1_2"/>
    <dbReference type="OMA" id="YEPSIWL"/>
    <dbReference type="OrthoDB" id="122167at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="64" mass="7638" checksum="DAA8B9F22C5ADA46" modified="2011-05-03" version="1">MKNKLKVYRAMNDLTQEDLAKEIKVTRQTIISIEKQKYDPSLTLAFKIAKFFNVHIEDIFFDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G2</accession>
    <name>F0T6G2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator ligand binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645947.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2383"/>
    <dbReference type="EnsemblBacteria" id="ADZ10596">
        <property type="protein sequence ID" value="ADZ10596"/>
        <property type="gene ID" value="Metbo_2383"/>
    </dbReference>
    <dbReference type="GeneID" id="10278849"/>
    <dbReference type="KEGG" id="mel:Metbo_2383"/>
    <dbReference type="eggNOG" id="arCOG03200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113664_0_0_2"/>
    <dbReference type="OMA" id="QMTGRVY"/>
    <dbReference type="OrthoDB" id="91997at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AraC_E_bind" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17150" checksum="BCFEE070EC8E8A64" modified="2011-05-03" version="1">MEILEKRIEETKVAYAQYKGSYSKIPAHLQEVGQLVIDDGLKMTGMVYGAYFNSPDEVPENELEYEIGFSFDGDLKLQEGMLGFKEIPEHTVLAAIHKGPYTEVGPVIQAIAKYADENGYDIVGPVTEVYLNDPSEVSAEELLTEVRFPVIKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6G9</accession>
    <name>F0T6G9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Elongation factor 2</fullName>
            <shortName evidence="7">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fusA</name>
        <name evidence="9" type="ordered locus">Metbo_2390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645954.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2390"/>
    <dbReference type="EnsemblBacteria" id="ADZ10603">
        <property type="protein sequence ID" value="ADZ10603"/>
        <property type="gene ID" value="Metbo_2390"/>
    </dbReference>
    <dbReference type="GeneID" id="10278857"/>
    <dbReference type="KEGG" id="mel:Metbo_2390"/>
    <dbReference type="eggNOG" id="arCOG01559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="OMA" id="GVMTQTE"/>
    <dbReference type="OrthoDB" id="1642at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="19"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="7">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="730" mass="81018" checksum="19830C510029F37C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6I8</accession>
    <name>F0T6I8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645973.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2409"/>
    <dbReference type="EnsemblBacteria" id="ADZ10622">
        <property type="protein sequence ID" value="ADZ10622"/>
        <property type="gene ID" value="Metbo_2409"/>
    </dbReference>
    <dbReference type="GeneID" id="10278881"/>
    <dbReference type="KEGG" id="mel:Metbo_2409"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_2"/>
    <dbReference type="OMA" id="CARMCPD"/>
    <dbReference type="OrthoDB" id="124457at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="7735" checksum="64DBA04B1457A8A3" modified="2011-05-03" version="1">MITVNEELCKGCNICKEFCPHNVYEESKALNKKGINVPCPKNKEQCTQCGLCTLLCPDQAIKVDDEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N5</accession>
    <name>F0T6N5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="8">ATP-PRT</shortName>
            <shortName evidence="8">ATP-PRTase</shortName>
            <ecNumber evidence="3 8">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisG</name>
        <name evidence="11" type="ordered locus">Metbo_0015</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643619.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0015"/>
    <dbReference type="EnsemblBacteria" id="ADZ08268">
        <property type="protein sequence ID" value="ADZ08268"/>
        <property type="gene ID" value="Metbo_0015"/>
    </dbReference>
    <dbReference type="GeneID" id="10276437"/>
    <dbReference type="KEGG" id="mel:Metbo_0015"/>
    <dbReference type="eggNOG" id="arCOG02208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="OrthoDB" id="56745at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="9">
        <location>
            <begin position="51"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="HisG_C" evidence="10">
        <location>
            <begin position="211"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="286" mass="30752" checksum="4EFD201F197BC7B6" modified="2011-05-03" version="1">MQIRIAVPSKGRISDPAVKLLAKAGIGIKDTANRKLFSETYDKEISVMFTRAADIPEFVADGAADIGMTGLDLIKENNADVEILEDLNFGSAKLVLAVPEESDIVSTTDISDGSVVATEFPNLTSKYLKSKGINAKIIELSGSTEIAPFIGVADIITDLTSTGTTLKMNHLKIIDTVLESSIKLIANKKSFNVNNEKIEAIRTGIKGVLDAEGKKLVMMNVEESFLEDVKKAMPGLTGPTVSSVLSSKDVVAVHAVVDEKDVFNLVNRLKKIGARDILVVPIERII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Q9</accession>
    <name>F0T6Q9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="8">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">ImGP synthase subunit HisH</fullName>
            <shortName evidence="8">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisH</name>
        <name evidence="11" type="ordered locus">Metbo_0039</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643643.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0039"/>
    <dbReference type="EnsemblBacteria" id="ADZ08292">
        <property type="protein sequence ID" value="ADZ08292"/>
        <property type="gene ID" value="Metbo_0039"/>
    </dbReference>
    <dbReference type="GeneID" id="10276461"/>
    <dbReference type="KEGG" id="mel:Metbo_0039"/>
    <dbReference type="eggNOG" id="arCOG00089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_2"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="OrthoDB" id="100772at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ08292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="203" mass="22001" checksum="C54ABE7B337084E9" modified="2011-05-03" version="1">MITIIDYGSGNLKSIKNGFSKIGTSTHISSSINEIKDADALVLPGVGAFGNAMEGVASYQEAIHDHIHDGKPFLGICLGLQILFTRSEESPDTTGLDVVEGSVLHFPDSIKESGLKIPQMGWNQLQIRGECPILKGIGSDFMYFVHSYYVAPDDPDVVVATVDYGIDVPAVICKDNVYATQFHPEKSGDKGLKILKNFVELVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S2</accession>
    <name>F0T6S2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0052"/>
    <dbReference type="EnsemblBacteria" id="ADZ08305">
        <property type="protein sequence ID" value="ADZ08305"/>
        <property type="gene ID" value="Metbo_0052"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0052"/>
    <dbReference type="eggNOG" id="arCOG10839">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3057126_0_0_2"/>
    <dbReference type="OMA" id="MGHVFER"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="6441" checksum="FB0F66BC40898228" modified="2011-05-03" version="1">MYSNKISRTDVLNKMGHVFERYRFLTRSKSFKQEQIQTIDYHLTEIMKVLNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S5</accession>
    <name>F0T6S5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_0055</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643659.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0055"/>
    <dbReference type="TCDB" id="1.A.78.2.6">
        <property type="family name" value="the k+-selective channel in endosomes and lysosomes (kel) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08308">
        <property type="protein sequence ID" value="ADZ08308"/>
        <property type="gene ID" value="Metbo_0055"/>
    </dbReference>
    <dbReference type="GeneID" id="10276478"/>
    <dbReference type="KEGG" id="mel:Metbo_0055"/>
    <dbReference type="eggNOG" id="arCOG04887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090238_3_2_2"/>
    <dbReference type="OMA" id="SALMSHY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="205" mass="24017" checksum="F31F6EFA29B8FD6E" modified="2011-05-03" version="1">MNTTNKHDVWLTTKRIETLVDGIFAIAMTLLVLNIDMPQIAGPVSNPAIWQYMLSLFQQLGIYAFSFILLASFWRAHHLQFFYIKRSDSALIWINVIWLMFVALVPFSTNFVSNYGNHPIPMLFFNINMFIIGIFFMLIWYYAKRKKFFIKEMTPEYYQTVKKINYILPSAALLAVCITFVSPTWSPVSYFLIFILKNAFKKGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6U9</accession>
    <name>F0T6U9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator PadR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644820.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09469">
        <property type="protein sequence ID" value="ADZ09469"/>
        <property type="gene ID" value="Metbo_1227"/>
    </dbReference>
    <dbReference type="GeneID" id="10277678"/>
    <dbReference type="KEGG" id="mel:Metbo_1227"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="184" mass="21571" checksum="AB006AC541581D64" modified="2011-05-03" version="1">MFVSHRDIRERMEELEKIGGLRVWILSVLSEGPKNGVEIADDIQFKHQKLHEVNFMDMNKRFEKMHNIKFPDNQTLEEHLSWRPSNGSLYPMLKKMVSEDLIVKNADGKYSLSEQGTETTYKLFGNLLKESYVDHNKITIKKAITELNGYISILEDFKPKKLSEHRDEIISLHERFGEIYSSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6X7</accession>
    <name>F0T6X7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644848.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1255"/>
    <dbReference type="EnsemblBacteria" id="ADZ09497">
        <property type="protein sequence ID" value="ADZ09497"/>
        <property type="gene ID" value="Metbo_1255"/>
    </dbReference>
    <dbReference type="GeneID" id="10277706"/>
    <dbReference type="KEGG" id="mel:Metbo_1255"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_1_0_2"/>
    <dbReference type="OMA" id="NDDTCAH"/>
    <dbReference type="OrthoDB" id="106121at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12241" checksum="15A47E46DAF76DA1" modified="2011-05-03" version="1">MDKFIWGILAGTKGTANRVRIIDELKNRPYNVNQLAEKLELDYKTVKHHIHVLETNHMVTSNGRKYGNLYFLSDKMEQNYDSFEEIKEEFIKSNDDTCAHVGHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6Z5</accession>
    <name>F0T6Z5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644866.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1273"/>
    <dbReference type="EnsemblBacteria" id="ADZ09515">
        <property type="protein sequence ID" value="ADZ09515"/>
        <property type="gene ID" value="Metbo_1273"/>
    </dbReference>
    <dbReference type="GeneID" id="10277724"/>
    <dbReference type="KEGG" id="mel:Metbo_1273"/>
    <dbReference type="eggNOG" id="arCOG01161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071768_0_0_2"/>
    <dbReference type="OMA" id="DCEGPLT"/>
    <dbReference type="OrthoDB" id="56355at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024196">
        <property type="entry name" value="NiFe_hyd_3_EhaR"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019370">
        <property type="entry name" value="EhaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="341" mass="37987" checksum="EB037D8D18A94AAA" modified="2011-05-03" version="1">MGRKFFVSDCEGPISINDNAFELAGHFIDDGEKFFEIISKYDDVLADVLKRPGYNAGGTLKLIVPFLKAFEANNENIINFSTENVHMINGAVETLNYIKNTMPSFIVSTSYNHYIQALCNVTGFPFENTYSTELDLDLVEISDTEKKTLKQFRSNIIKNPDFDVLEDIFWNKIPKLKSGSLMNMVHPVGGEGKKDAVLDIISKNGFNPSDLFYIGDSITDVQPLRFARENNGISVSFNGNEYAINESEIAVLSENTTITSILADIFNTHGTSTVLEFVELYSENPTKSLEADYVNGDLKNTITKSKLPTVEIVNTDNQEQLIKSSTIIRRKLRGESIGGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T718</accession>
    <name>F0T718_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644889.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09538">
        <property type="protein sequence ID" value="ADZ09538"/>
        <property type="gene ID" value="Metbo_1296"/>
    </dbReference>
    <dbReference type="GeneID" id="10277747"/>
    <dbReference type="KEGG" id="mel:Metbo_1296"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184949_0_0_2"/>
    <dbReference type="OMA" id="VEWDFEA"/>
    <dbReference type="OrthoDB" id="128235at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="8604" checksum="17608855A1D916FF" modified="2011-05-03" version="1">MKIDDDLLGKEVIDASGDKVGTVKEVDWDFQTKKVEFIELEEAGISSKIGLGDKRVVPINDVKEIGDKVLIKTRLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T728</accession>
    <name>F0T728_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease III Xth</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2449</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646013.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2449"/>
    <dbReference type="EnsemblBacteria" id="ADZ10662">
        <property type="protein sequence ID" value="ADZ10662"/>
        <property type="gene ID" value="Metbo_2449"/>
    </dbReference>
    <dbReference type="GeneID" id="10278921"/>
    <dbReference type="KEGG" id="mel:Metbo_2449"/>
    <dbReference type="eggNOG" id="arCOG02207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_3_1_2"/>
    <dbReference type="OMA" id="WWSYRGR"/>
    <dbReference type="OrthoDB" id="50766at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="PTHR22748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="257" mass="30074" checksum="395041EE216DE1D0" modified="2011-05-03" version="1">MDKIRILSWNVNGIRAVHKKGFLDWFNNEKPDILCIQETKAVRKQFPPDIRAVDGYQLYVSEASRKGYSGTATYTSLKPEGVKYGFGVEKFDTEGRTLITDYGDFVLFNIYFPNGKMSKERLDYKMEFYDTFLEYADELKDQGKNIVVCGDVNTAHREIDLAHPKENSKISGFLPEERAWIDKLLDHGYVDTFREFNQEPEEYTWWSYRTRARERNVGWRLDYFFVNRDFMKNVSAAYILPEVMGSDHCPVGIDVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T753</accession>
    <name>F0T753_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PKD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2474"/>
    <dbReference type="EnsemblBacteria" id="ADZ10687">
        <property type="protein sequence ID" value="ADZ10687"/>
        <property type="gene ID" value="Metbo_2474"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2474"/>
    <dbReference type="eggNOG" id="arCOG02510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260893_0_0_2"/>
    <dbReference type="OMA" id="STLYTWP"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="1">
        <location>
            <begin position="353"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1308" mass="142357" checksum="038A78296ACB8D04" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0T763</accession>
    <name>F0T763_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Glutamine synthetase</fullName>
            <ecNumber evidence="2 5">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_2484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="2 5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646048.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2484"/>
    <dbReference type="EnsemblBacteria" id="ADZ10697">
        <property type="protein sequence ID" value="ADZ10697"/>
        <property type="gene ID" value="Metbo_2484"/>
    </dbReference>
    <dbReference type="GeneID" id="10278958"/>
    <dbReference type="KEGG" id="mel:Metbo_2484"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
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    <dbReference type="OrthoDB" id="47310at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="6">
        <location>
            <begin position="107"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="442" mass="49839" checksum="C4F8107065FB00C3" modified="2011-05-03" version="1">MEDKIGKVIENIERCGTKFIRLQFVDIHGTPKNMAVPISKPEDIEDILKDGLLFDGSSVDGFVDINASDLIIKPDPDTFSTLPWRPEEKGVCRFICDIYWPDMTPFEGDPRYILKKALAKIEDKGYEYNVGPEPEFFIIGEDAEGHIVPHDNGIYFDVEPVDQGTDVRRELVLGLEELNFEVEVSHHEVGPGQHEIDFKFDHAMKTADAVITFKQAIKAIVDNLGYMVTFMPKPFFGVNGSGMHVHQSLFKNGKNIFYDPDGQDQLSEEARYFIGGLLKHSKALSAIVAPTVNSYKRLVPGYEAPCYIAYGLKNRSTLVRIPASRGNGTRVEFRCPDPSCNPYLAFAAMLESGMDGLNNKIDPGEATEIDVFGLDAAGLEREGIETLPSSLWEAYHALEQDDVVKSSLGDHVYKQFMDIKKKEWDDYRIQVFPYELEKYLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T777</accession>
    <name>F0T777_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646062.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2498"/>
    <dbReference type="EnsemblBacteria" id="ADZ10711">
        <property type="protein sequence ID" value="ADZ10711"/>
        <property type="gene ID" value="Metbo_2498"/>
    </dbReference>
    <dbReference type="GeneID" id="10278972"/>
    <dbReference type="KEGG" id="mel:Metbo_2498"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="OMA" id="IAIMDYK"/>
    <dbReference type="OrthoDB" id="67424at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="37"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="204" mass="23037" checksum="F2DD04F063CCB181" modified="2011-05-03" version="1">MSNKTGLMPFNGHRIQGRSSESFIDAREVISRLKLKGDEVFLDAGCGDGHTAFEAYDMMNQDAVIYAVDIYEPSIQDLEEEIEAKKITRIVPVLGNIAGDIPIPDNTLDMTLMINVFHGFVAQENLHEAVEELKRITKPCGKIAIMDYKKKETKYGPPFFVRQSPEELEDLFKGHDLKLTEVDNQIGETLEDGSKSHYLVIFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T788</accession>
    <name>F0T788_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646073.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2509"/>
    <dbReference type="EnsemblBacteria" id="ADZ10722">
        <property type="protein sequence ID" value="ADZ10722"/>
        <property type="gene ID" value="Metbo_2509"/>
    </dbReference>
    <dbReference type="GeneID" id="10278983"/>
    <dbReference type="KEGG" id="mel:Metbo_2509"/>
    <dbReference type="eggNOG" id="arCOG04461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035587_0_0_2"/>
    <dbReference type="OMA" id="GEKMDIC"/>
    <dbReference type="OrthoDB" id="8152at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="40"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="569" mass="63301" checksum="3B7C581724DAEA61" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7F0</accession>
    <name>F0T7F0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Agmatinase</fullName>
            <ecNumber evidence="5">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0126</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        </source>
    </evidence>
    <sequence length="306" mass="33892" checksum="0D20D274CCD569B0" modified="2011-05-03" version="1">MNFNDIESRFPYIGVSTFYGYPNSKDLNETDVAIVGVPFDHGTTNRPGARFGPRSIRTASQNYGIYLNEFGAFDSELKRNILGGVNIVDYGDVPILPTHTKTNMKMINSTFKKIVDAGVFPVAFGGDHTVTYPIVKAFKKPLDIVHFDTHLDFVDGTENLKFSHSNPLKRVSEIENINNITQIGIRGFTDKIENYDESKNYGSKIITASEVMDRGTAWVLDQIPESDNIYVTLDIDVLDPSIAPGTGTPEPGGLTYNQLKDILTNLTNKGEILGFDLVEVNPLFDLSEITSLTAARLCFDFLGSIF</sequence>
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    <reference evidence="3" key="1">
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="Metbo_1311"/>
    </dbReference>
    <dbReference type="GeneID" id="10277762"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="16665" checksum="1543F261B59B5CAC" modified="2011-05-03" version="1">MRSDPIEKYINNVTKNMGSKQREEVARELKAHIWDSADALAEEKHVEVDDTIIQDTITRMGPAEDIAAMYPKEKAGKDKIIDGLKYIARSLIFFIVAAGIIGIVLQLIFKDISIYPFVVVAIIVYLVIMSIHFVRRKFIPKFLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7I9</accession>
    <name>F0T7I9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644908.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1315"/>
    <dbReference type="EnsemblBacteria" id="ADZ09557">
        <property type="protein sequence ID" value="ADZ09557"/>
        <property type="gene ID" value="Metbo_1315"/>
    </dbReference>
    <dbReference type="GeneID" id="10277766"/>
    <dbReference type="KEGG" id="mel:Metbo_1315"/>
    <dbReference type="eggNOG" id="arCOG00568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023226_0_0_2"/>
    <dbReference type="OMA" id="HYTTAYV"/>
    <dbReference type="OrthoDB" id="43014at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018701">
        <property type="entry name" value="DUF2206_membrane"/>
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    <dbReference type="Pfam" id="PF09971">
        <property type="entry name" value="DUF2206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="32"/>
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    <feature type="transmembrane region" description="Helical" evidence="1">
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        <location>
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            <end position="364"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="416"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="705" mass="79881" checksum="48BC09AC5331D01F" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J8</accession>
    <name>F0T7J8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644917.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09566">
        <property type="protein sequence ID" value="ADZ09566"/>
        <property type="gene ID" value="Metbo_1324"/>
    </dbReference>
    <dbReference type="GeneID" id="10277775"/>
    <dbReference type="KEGG" id="mel:Metbo_1324"/>
    <dbReference type="eggNOG" id="arCOG10257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184180_0_0_2"/>
    <dbReference type="OrthoDB" id="110819at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10906" checksum="24692773A781B704" modified="2011-05-03" version="1">MDNKKRNELKIKCESMKDDPSLMKECTVFLKTMEDKKVGMKDDSGQNYTNMVENISAEDVPKVLEMALKIARSDEIDDPELKNAAERLIRTLEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L0</accession>
    <name>F0T7L0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644929.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1337"/>
    <dbReference type="EnsemblBacteria" id="ADZ09578">
        <property type="protein sequence ID" value="ADZ09578"/>
        <property type="gene ID" value="Metbo_1337"/>
    </dbReference>
    <dbReference type="GeneID" id="10277788"/>
    <dbReference type="KEGG" id="mel:Metbo_1337"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OMA" id="HNPTDIM"/>
    <dbReference type="OrthoDB" id="76011at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="234" mass="26004" checksum="FB530CCBD9C624FE" modified="2011-05-03" version="1">MIACIESGIPGFDELTRTDSFMGGIPENSSTLVYGPPKTGKSIFSDQFCFNGLVNEEPCLYITTNKGLKMLKSDMNDYNLSVDEYMMNGSLHIIDTISDILEKKVEPTGTVVNSEINNPTDLMVNIGSKINVVRNQNSRFRSVLNSATTLLTYNESMLIVRVIKAYLMRIKEAGGTSLITYTEDSADNIIETMLKSMVDNIIRMDGNELQVEAMKGFGKIKTNYTITEDGIMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7P7</accession>
    <name>F0T7P7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644966.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1375"/>
    <dbReference type="EnsemblBacteria" id="ADZ09615">
        <property type="protein sequence ID" value="ADZ09615"/>
        <property type="gene ID" value="Metbo_1375"/>
    </dbReference>
    <dbReference type="GeneID" id="10277826"/>
    <dbReference type="KEGG" id="mel:Metbo_1375"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_13_2_2"/>
    <dbReference type="OMA" id="NMEGGIT"/>
    <dbReference type="OrthoDB" id="116946at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="116" mass="13413" checksum="ABA40DFB97D26B2C" modified="2011-05-03" version="1">MFGYGKKKDYDINILDAYDLIVENQSKPKFMILDVRTPQEFAESRIENAKNIDYNSNTFKNEVSKLERDGKYLVYCRSGMRSLNATKIMMDLGFTDVKNMEGGITKWINKGLPIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7R6</accession>
    <name>F0T7R6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0151"/>
    <dbReference type="EnsemblBacteria" id="ADZ08403">
        <property type="protein sequence ID" value="ADZ08403"/>
        <property type="gene ID" value="Metbo_0151"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0151"/>
    <dbReference type="eggNOG" id="arCOG01462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_728844_0_0_2"/>
    <dbReference type="OMA" id="EIMYGFI"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="380" mass="41922" checksum="70CBACAC973D654B" modified="2011-05-03" version="1">MNKLKALTVKEGNDILRNKIYLLVVLVQVFILLGAFGLALASSVASDPALLDEYGATSVLKVAVPETVKGTPLDTALIDQKLNITYYNNVTVAQNALGTGIIAVVKVDPNNSLNISVDSDNSNIFYPIVSSKISAAVNQYSIEEKLKSSGVNSSQIQQILSPVNLQVIVYNMDKQSKLALNSPFFVEIMYGFIVPFILLLPFFLASNIVTDSVVGERERKTFEVLLMTPVSSSQVMLGKIIPILSFSMIQSMIWILLLDLLKVPIYNSVPILLILFFVGLAFIGIGTLLSMFVDSTKEANSAITIVLVFATFVLFMPLFIKTTYFSAILNFIPTVLIVKMASTPNISLEMLIYSLPTVLISILIFMLTIRYFRHERAIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7S4</accession>
    <name>F0T7S4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Metbo_0159"/>
    </dbReference>
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    <dbReference type="KEGG" id="mel:Metbo_0159"/>
    <dbReference type="HOGENOM" id="CLU_2204134_0_0_2"/>
    <dbReference type="OrthoDB" id="137737at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="107" mass="11798" checksum="5651FCDDA4BF019D" modified="2011-05-03" version="1" precursor="true">MKPQNRQFVIYIAGIFLLISGIVGILLPSLGLSLFTSVVWAVLGAVFLGLAHGTKLRKIVLYAAGIFLFVSGILGFLYPQLELTTLNSIIWIILGVLFMYISYSVKY</sequence>
</entry>
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    <accession>F0T807</accession>
    <name>F0T807_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
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    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644984.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09633">
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        <property type="gene ID" value="Metbo_1393"/>
    </dbReference>
    <dbReference type="GeneID" id="10277844"/>
    <dbReference type="KEGG" id="mel:Metbo_1393"/>
    <dbReference type="HOGENOM" id="CLU_1891444_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="15743" checksum="8A0710B770BADA7F" modified="2011-05-03" version="1">MIKISQNKYKIPDEYRRKILSGYFSIALIILGTILIPRFYVFMNLNFQIILTLITLIPGAILWIYFFNGFIRNLVDLIKSSGSMKNETTLKTHYFLIWIINIIWIVLAHFIYNISLSGILIPIISIILLIKFMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T820</accession>
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        <submittedName>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644997.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09646">
        <property type="protein sequence ID" value="ADZ09646"/>
        <property type="gene ID" value="Metbo_1407"/>
    </dbReference>
    <dbReference type="GeneID" id="59558431"/>
    <dbReference type="KEGG" id="mel:Metbo_1407"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="121662at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="159" mass="18355" checksum="50F9C2BEFF0A0D89" modified="2011-05-03" version="1">MKQVGELRMWILHVLDETGPSNGVEIMANVQAHYDLQVQWHNDTHKHPHPETDIKSKKLLPGSIYPMLKKMVSEGLITKKEDGRYELTKKGHIVTTNLFEHFQSQKNGDPLPISLKNSLTAINWHVSYMEDANKEDITPYQDSIESLIERLEKLKKSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>F0T862</accession>
    <name>F0T862_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09688">
        <property type="protein sequence ID" value="ADZ09688"/>
        <property type="gene ID" value="Metbo_1451"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1451"/>
    <dbReference type="HOGENOM" id="CLU_2730409_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="8279" checksum="6B05FBCFC1E39026" modified="2011-05-03" version="1">MFSKNAVKYFLNFSVFLRLRMWLGLSIVLRSELGTLSLIILDDESGINLSFSPITWEFFRDCTGSDNEFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T879</accession>
    <name>F0T879_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-amino-6-(5-phosphoribosylamino) uracil reductase</fullName>
        </submittedName>
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    <gene>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643842.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0239"/>
    <dbReference type="EnsemblBacteria" id="ADZ08491">
        <property type="protein sequence ID" value="ADZ08491"/>
        <property type="gene ID" value="Metbo_0239"/>
    </dbReference>
    <dbReference type="GeneID" id="10276669"/>
    <dbReference type="KEGG" id="mel:Metbo_0239"/>
    <dbReference type="eggNOG" id="arCOG01489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073038_2_0_2"/>
    <dbReference type="OMA" id="YVICHMM"/>
    <dbReference type="OrthoDB" id="58608at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="3"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="23856" checksum="ECAD8FEBB8CA7C29" modified="2011-05-03" version="1">MQPKVILYIPMSLDGKTSGFEINMNVYKEIKSDINSDAVFMDVEAFKTSYTSIIDSSSKQLLVLPDNMGKIPRSMLLERTSKMNVLVLCSRSTPQDYLNFLEENYINHMIVGYDEVNMATAFEELNIQFGVKTIAVHADGVLNSNLMIEDLVEEISIFMHPTLAGANNDDMFLQNNPENQKLDLRLLETKVVEDEIVYLKYRIMKYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T884</accession>
    <name>F0T884_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Potassium uptake protein, TrkH family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643847.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0244"/>
    <dbReference type="EnsemblBacteria" id="ADZ08496">
        <property type="protein sequence ID" value="ADZ08496"/>
        <property type="gene ID" value="Metbo_0244"/>
    </dbReference>
    <dbReference type="GeneID" id="10276676"/>
    <dbReference type="KEGG" id="mel:Metbo_0244"/>
    <dbReference type="eggNOG" id="arCOG04145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="OrthoDB" id="13996at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="479" mass="52948" checksum="5D241D7EDCE1C423" modified="2011-05-03" version="1">MIAYLRRKDFYLIFKNLGTVMEGIGIVMLIPLIVALIYGENDFAGFLVAGILSMSIGYVLKIKFSDSINLKLKHGMIIAALAWLWAALMGSICLMYATDISFLNAYFESMSAWTGSGLTMYTNVQILPQSVLFLRSLEQWVGGLGVVIVVIGILIRPGTAASRLYKAEAREEKIKPSIANTVRTIWWIYLFYTILGIVLYILAGMSLFDAINNCFTNLSTGGMSIKNDNIGFYNSVPITIVTILLMILGGTSFLVHYKALKGRIWDVFRDIQFQAMVIIVAVFSIMLFTVSHFTSLNSIFYIVSALSCTGSNTVTAGTMFGWTDYTKVILIVCMIIGMAAGSTTGALKLIRVVTLIKGIYWEVVKVLSPEGSVFPRKISGKTVKDAEIKEAGSYTFLYLFFILITWFIFLSYGYGGIDSLFEIASAQGNVGLSVGITGPTMPKLPEILLIIGMWIGRIEIIPALVLLKGSFDLFKRFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T885</accession>
    <name>F0T885_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643848.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0245"/>
    <dbReference type="EnsemblBacteria" id="ADZ08497">
        <property type="protein sequence ID" value="ADZ08497"/>
        <property type="gene ID" value="Metbo_0245"/>
    </dbReference>
    <dbReference type="GeneID" id="10276677"/>
    <dbReference type="KEGG" id="mel:Metbo_0245"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="OMA" id="NAVLYFR"/>
    <dbReference type="OrthoDB" id="49614at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="PTHR10264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="3">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="260" mass="28959" checksum="F408A1F917B7F05D" modified="2011-05-03" version="1">MDLFTLIIIGIIILIILGLSIRVVNQYERGVVFRVGKVIGVKEPGLRLIIPVVDRMVKASLQIVTMPIPSQKIITEDNVSIDVAAVAYFKIMDPYKAVVEVENYNRAVNQISQTTVRSVVGQFNLDEILSETPKINTKIKEIIDKHSEPWGINVTTVEIKDIKLPDTMKRVIAMQAEAEREKRAKIIAAEGEYLSAAKLGDAADIISEHPIALQLRIMQVLNQIAVEKNSTIIFPAPLMNSITDISNFLKTENMGAEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C2</accession>
    <name>F0T8C2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643885.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08534">
        <property type="protein sequence ID" value="ADZ08534"/>
        <property type="gene ID" value="Metbo_0282"/>
    </dbReference>
    <dbReference type="GeneID" id="10276714"/>
    <dbReference type="KEGG" id="mel:Metbo_0282"/>
    <dbReference type="eggNOG" id="arCOG10257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184180_0_0_2"/>
    <dbReference type="OMA" id="VEMEDMP"/>
    <dbReference type="OrthoDB" id="110819at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="95" mass="10719" checksum="25D11AB26D0760C1" modified="2011-05-03" version="1">MDDKKTNELMAKCENNKDDPALMKECKQMLNTMAENNVEMEDMPNQSYTNMAENISAEDVPKVLAMALKIAKSGDIQDTELKIAAERLIRTLEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E7</accession>
    <name>F0T8E7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1461</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulator TrmB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1461"/>
    <dbReference type="EnsemblBacteria" id="ADZ09698">
        <property type="protein sequence ID" value="ADZ09698"/>
        <property type="gene ID" value="Metbo_1461"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1461"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072493_0_1_2"/>
    <dbReference type="OMA" id="YETQIWI"/>
    <dbReference type="OrthoDB" id="72347at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="2">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Regulator_TrmB" evidence="3">
        <location>
            <begin position="122"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="266" mass="29876" checksum="109FE2AD2446F119" modified="2011-05-03" version="1">MDFNGGFKISVNRNTVTALKNLGLSDYEIKAYVANISLVSATASEISLASNVPRSKIYEVLKSLAKKDFVEIESGKPLKFNAIPPHEIIGKSRKQFNENLDEAEAELNQIYEKQIPNVPAPLWLIHGPDKILKKEVEIISRAKKSIYIIAGLLFKDEIPDLKDSLDKAINRGVHVKIILSPFYNDGGDVDLECIRGLECEIKTLNVPLVKVIIRDDKEMLMGVAKIEDKKIMSSSAIGIWNQYTELVEAIEGLYNFIWTTELFNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8F1</accession>
    <name>F0T8F1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645053.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1465"/>
    <dbReference type="EnsemblBacteria" id="ADZ09702">
        <property type="protein sequence ID" value="ADZ09702"/>
        <property type="gene ID" value="Metbo_1465"/>
    </dbReference>
    <dbReference type="GeneID" id="10277916"/>
    <dbReference type="KEGG" id="mel:Metbo_1465"/>
    <dbReference type="HOGENOM" id="CLU_132548_1_0_2"/>
    <dbReference type="OrthoDB" id="107832at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14208" checksum="A900DEBBBD1DB6FC" modified="2011-05-03" version="1">MSKNVADERIQILGMVEEGKITAAEGMELLNALEENNAEIVPKTEAKWLKVRVKTMDEEKTKVNVNIPIALVDVGLKLAKNYDPKLKDSGLDKIDIEEIVEAVKNGAEGKIVDVEDEESQTNVQVYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8K4</accession>
    <name>F0T8K4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PsbP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645106.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09755">
        <property type="protein sequence ID" value="ADZ09755"/>
        <property type="gene ID" value="Metbo_1525"/>
    </dbReference>
    <dbReference type="GeneID" id="10277976"/>
    <dbReference type="KEGG" id="mel:Metbo_1525"/>
    <dbReference type="eggNOG" id="arCOG06468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363655_0_0_2"/>
    <dbReference type="OrthoDB" id="71973at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="PsbP" evidence="2">
        <location>
            <begin position="57"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="200" mass="21878" checksum="52C4EF081F3E963C" modified="2011-05-03" version="1">MQKKYIGLILTISIIVILSVGYITLNTFGVSDSSFISGSGSSSGSVTSVDMDGNTYSGDGVSFDIPSHWQVYKVVDGTNININIVKNYSDTTQITIVIAPNPKGLSNQELIDSIKDPVNEGDGNWVKISNNTTSINGTTAYENTYTVSNLSQFKGSLIIKEINFIKNGYTYGFTFQAPVNEFNNEQTNFNITLNSFKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8S9</accession>
    <name>F0T8S9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_013643972.1">
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    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0369"/>
    <dbReference type="EnsemblBacteria" id="ADZ08621">
        <property type="protein sequence ID" value="ADZ08621"/>
        <property type="gene ID" value="Metbo_0369"/>
    </dbReference>
    <dbReference type="GeneID" id="10276806"/>
    <dbReference type="KEGG" id="mel:Metbo_0369"/>
    <dbReference type="eggNOG" id="arCOG02472">
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    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
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    <dbReference type="OrthoDB" id="44934at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0051536">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
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        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
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        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="15"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="309" mass="33213" checksum="48A0A1E8808C989D" modified="2011-05-03" version="1">MADKPKIGTMWLAGCSGCHLSIADIHEAIIDVMGLADFEFSPVLMDVKYDEVPDDLDVLIIEGGIRNDENRELAEMLRKKAKFVIAYGVCAVYGGIPGLGNLHTADELAQEAYVNSASTYNEEGIIPNEDIPHLESRVRPLSDVIDVDLSIPGCPTRSDVLAEALVALLTGEAVSLPTTNLCEVCPREKPPEGMAMDKIIRQFELGRPDEELCLVPQGLVCMGPSTTSLCGAECPSIGIPCRGCYGPTAKVTDQGAKMISAIGSDFGVEHDKTVDPEEVADQLDDIVGTFYTYTLPAALIPMKVQKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8U3</accession>
    <name>F0T8U3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyguanosinetriphosphate triphosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1541"/>
    <dbReference type="EnsemblBacteria" id="ADZ09771">
        <property type="protein sequence ID" value="ADZ09771"/>
        <property type="gene ID" value="Metbo_1541"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1541"/>
    <dbReference type="eggNOG" id="arCOG07445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028163_1_0_2"/>
    <dbReference type="OMA" id="EFSRDRD"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016793">
        <property type="term" value="F:triphosphoric monoester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3550.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dGTP_triphosphohydrolase_C"/>
    </dbReference>
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        <property type="entry name" value="dNTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01353">
        <property type="entry name" value="dGTP_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="86"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="661" mass="78561" checksum="511440C4711CD33D" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V5</accession>
    <name>F0T8V5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645134.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1556"/>
    <dbReference type="REBASE" id="66882">
        <property type="entry name" value="Msp21ORF1556P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09783">
        <property type="protein sequence ID" value="ADZ09783"/>
        <property type="gene ID" value="Metbo_1556"/>
    </dbReference>
    <dbReference type="GeneID" id="10278012"/>
    <dbReference type="KEGG" id="mel:Metbo_1556"/>
    <dbReference type="eggNOG" id="arCOG02636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04814">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270280_0_0_2"/>
    <dbReference type="OMA" id="CKSAIFI"/>
    <dbReference type="OrthoDB" id="15235at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RM_methylase_Eco57I-like"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="Eco57I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eco57I" evidence="3">
        <location>
            <begin position="606"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1059" mass="124807" checksum="175F95CA9628DC77" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V8</accession>
    <name>F0T8V8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645137.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1559"/>
    <dbReference type="REBASE" id="33853">
        <property type="entry name" value="Msp21McrBP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09786">
        <property type="protein sequence ID" value="ADZ09786"/>
        <property type="gene ID" value="Metbo_1559"/>
    </dbReference>
    <dbReference type="GeneID" id="25394735"/>
    <dbReference type="KEGG" id="mel:Metbo_1559"/>
    <dbReference type="eggNOG" id="arCOG07794">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274255_0_0_2"/>
    <dbReference type="OrthoDB" id="43492at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021961">
        <property type="entry name" value="McrB_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12102">
        <property type="entry name" value="MrcB_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MrcB_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="MrcB_N" evidence="1">
        <location>
            <begin position="359"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1169" mass="136785" checksum="E2706E6DFA60E797" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8X4</accession>
    <name>F0T8X4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645153.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1575"/>
    <dbReference type="EnsemblBacteria" id="ADZ09802">
        <property type="protein sequence ID" value="ADZ09802"/>
        <property type="gene ID" value="Metbo_1575"/>
    </dbReference>
    <dbReference type="GeneID" id="10278031"/>
    <dbReference type="KEGG" id="mel:Metbo_1575"/>
    <dbReference type="HOGENOM" id="CLU_1665515_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="158" mass="17636" checksum="0CB608C4C5E3BCB1" modified="2011-05-03" version="1" precursor="true">MKKYNGSIFGIVVILILVIMVSGCTSSSSQQNGTKIYQRNWKVSADHFGGVSQEVIDAFAGSYVAYKNGTTITLIGTGKQLSKENDTMHQLKEVYVQDGSNVNTTYYIDGRLGGYSYTNQTTTDQMFDISKKTWELSLTTVEENLSMELDNRTENVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T929</accession>
    <name>F0T929_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0399"/>
    <dbReference type="EnsemblBacteria" id="ADZ08651">
        <property type="protein sequence ID" value="ADZ08651"/>
        <property type="gene ID" value="Metbo_0399"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0399"/>
    <dbReference type="eggNOG" id="arCOG03622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084675_0_0_2"/>
    <dbReference type="OMA" id="WEPYFEE"/>
    <dbReference type="OrthoDB" id="64254at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="313" mass="35274" checksum="0F1D2156BA934CCC" modified="2011-05-03" version="1" precursor="true">MKSGKISSSIIIVTMLVLFISISAVAAHQPRLETGINTSMSNPIIIENPEISQAFYSTLHGSPDYYKISSDVPFKFYLNLLVPQSPGIQADFVSAQVLDTNGNILLTLNGTNSTWEPYFEEFGGDYYLKGPEVTEQLPAGDYYIKIFNAENQGKYNIAVGETESFPIDESLKALVTIPLLKEQFFGKPVTNLFLEFLGLIFALGSTMVLYFMLIKARKSEEITQLTIKVYSLLKPMMWIGILITAVVWFYVMYKDPFNIVGIVNSLIFVVILLLSWYNGRKFATIQLNKLPLFSMTLLVVLWWLFVYASIAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T944</accession>
    <name>F0T944_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644017.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0414"/>
    <dbReference type="EnsemblBacteria" id="ADZ08666">
        <property type="protein sequence ID" value="ADZ08666"/>
        <property type="gene ID" value="Metbo_0414"/>
    </dbReference>
    <dbReference type="GeneID" id="10276851"/>
    <dbReference type="KEGG" id="mel:Metbo_0414"/>
    <dbReference type="eggNOG" id="arCOG09726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197250_0_0_2"/>
    <dbReference type="OMA" id="TAMNSDV"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="68" mass="7086" checksum="8C62722F3A982390" modified="2011-05-03" version="1">MNIYDDEAAQSSVELILLFGGVIAIVVVGAAFYKNYLSGLGGNITSNDLSSVTNSINGTGNSLINKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T989</accession>
    <name>F0T989_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Polymorphic membrane protein Chlamydia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645191.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09840">
        <property type="protein sequence ID" value="ADZ09840"/>
        <property type="gene ID" value="Metbo_1613"/>
    </dbReference>
    <dbReference type="GeneID" id="10278070"/>
    <dbReference type="KEGG" id="mel:Metbo_1613"/>
    <dbReference type="eggNOG" id="arCOG09729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775257_0_0_2"/>
    <dbReference type="OMA" id="GGAIHNY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="357" mass="37839" checksum="8A4DADC872ACB536" modified="2011-05-03" version="1" precursor="true">MKNLKVVFIPLIILIILGTASASQATNASQTVNNGGKLVQSSNPTSNDLSVYYVDMKGNDNNTGKNLKQAFKTIQKAINVGSGNFCVVVAKGVYFENLVINKNVKLIGNSSKNTVINGNNKSSCVWIKNSGDVQIDGLTFTNGKSSLGGGIRNQGTLTLKYSCVTSNRANDGGGIYNEGTVNIRGSSISNNSCNYNGGGIQNLNTAYIMRTNILSNTAEMGGGINSKGELQLYQSRVSNNKAEIGGGINNDHCVISTVSSYINGNKAIEGGGIYNNNGIFDIFVTQINSNVASYGGGISNYGLINLHFSTVTKNYASIKGGGIYNYSSIFKDISTTITNNTIDNVNLNPVHNFPVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9C5</accession>
    <name>F0T9C5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Proteinase inhibitor I42, chagasin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645227.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09876">
        <property type="protein sequence ID" value="ADZ09876"/>
        <property type="gene ID" value="Metbo_1650"/>
    </dbReference>
    <dbReference type="GeneID" id="10278107"/>
    <dbReference type="KEGG" id="mel:Metbo_1650"/>
    <dbReference type="eggNOG" id="arCOG03545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032882_0_0_2"/>
    <dbReference type="OrthoDB" id="143700at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036331">
        <property type="entry name" value="Chagasin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141066">
        <property type="entry name" value="SSF141066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="125" mass="13850" checksum="1C409342E1188C1E" modified="2011-05-03" version="1" precursor="true">MNHTKNFFGLLIAFMCLAMISGSSAANPDTKNIDQGGAFPCYKLTINLVCNPSTGYMWVPEFNSSEVKWINDSYIDGSGLIGEDKLEQFVFVGVTGAKVVMKLENPNKQVVAIKTYKIPGFFFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G2</accession>
    <name>F0T9G2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0458"/>
    <dbReference type="EnsemblBacteria" id="ADZ08710">
        <property type="protein sequence ID" value="ADZ08710"/>
        <property type="gene ID" value="Metbo_0458"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0458"/>
    <dbReference type="eggNOG" id="arCOG04469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043916_0_0_2"/>
    <dbReference type="OMA" id="FVGCAML"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="449" mass="49069" checksum="D54491FA3B60963D" modified="2011-05-03" version="1">MYTGIRFTIFKVVILIYQLIIITVRSIKYTCGDKILDLLLACVFGVLVGVFTGMVPGIHVNTVAAFVFSSSVYLMNYFSPEYLAVFLISLSISHSLIDFIPTMFLGVPDEGTALSVLPGHYLMLQGRGKEAIRLVTLGGFGSLMVTVTLLPIFIMLLPGFYSIIKPYIALILTFTAIYMVLRLNKGIYEIFWSSVIFIFSGIIGWASLNSTVSPNVLLLTIFSGFFGVSTLIYSLSQNSYLPYQHENHNLRVDGKIIRGIFAGGIAGTILGFLPGMGPAQGSILAQEMSGGGNMTEGRESFLVAMSGVNVSDALFSLITIYIIGNPRSGVAVFIDKLIQNFDLQILVMFVFVSVTAVSISVFFCISLGDFMIKNIHKLNYNKLSRGIIILMSALVIIFTLKEGSNLFYVIILYITSISLGLIPHYVGVNKSNLMGVLIVPAIVIYTGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9G3</accession>
    <name>F0T9G3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644062.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0459"/>
    <dbReference type="EnsemblBacteria" id="ADZ08711">
        <property type="protein sequence ID" value="ADZ08711"/>
        <property type="gene ID" value="Metbo_0459"/>
    </dbReference>
    <dbReference type="GeneID" id="10276897"/>
    <dbReference type="KEGG" id="mel:Metbo_0459"/>
    <dbReference type="eggNOG" id="arCOG01220">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069059_0_0_2"/>
    <dbReference type="OMA" id="VHIEFPR"/>
    <dbReference type="OrthoDB" id="60331at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="326" mass="36419" checksum="EEDFC9CB7438B01F" modified="2011-05-03" version="1">MEIQCGCGLGCVEKADTILNGITEINRPCENCLRPNLKKFKPLMEQMDLEELDANYGRCNCKKRHLDLVMGHVLKILIETGDRDKKSTLRNCCTPLITPAYPSNTAPYLSENSVVILTDKVTKESAVKIVKEVPEIKGVIRGDIKRVVGIKDANSKPNVYELLSGCDMRCDIVSTQHGPICIYRKQSQIHLEFSKPVPPKVQMLEKYLDKYENPRVMDCTCGPGTLGIASLKHGAKHVVFNDLWHPSIEMTVLNLEVNGFEVESLHSKGSLVAEGKNFKVYCADIMELKDDIDEKFDLCIVDTFPGVDNDEFVEAVSKICSEVVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9I1</accession>
    <name>F0T9I1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644080.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0477"/>
    <dbReference type="EnsemblBacteria" id="ADZ08729">
        <property type="protein sequence ID" value="ADZ08729"/>
        <property type="gene ID" value="Metbo_0477"/>
    </dbReference>
    <dbReference type="GeneID" id="10276917"/>
    <dbReference type="KEGG" id="mel:Metbo_0477"/>
    <dbReference type="eggNOG" id="arCOG00213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_5_1_2"/>
    <dbReference type="OMA" id="FKKKALC"/>
    <dbReference type="OrthoDB" id="40323at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="27"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="281" mass="29087" checksum="7DA92AD010CB7E0B" modified="2011-05-03" version="1">MDKKYIIGIIIALVVIVGAYMVVAPGSGSKVTVVGSTSVQPVAEKLATAYMKEHPNVKITVQGGGTAVGIKSAQDGTANIGTASKALSTNDSQGLTQYTLGQDGVVAIVSPNNNLTGLTKDQLKGIFSGNITNWNQVGGPDAKINVIVREEGSGTRDAFQDIVLGKLSNGTKVSFVSSAIVQSSTQAVQQAVSQDPNAIGFCSFADLGNDTKALSINGISASASTIADGTYTIQRPFLFLVKGDAKGEVKNFIDWVMGPEGQAILTSQKIIKSTNSTNSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N1</accession>
    <name>F0T9N1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645261.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1685"/>
    <dbReference type="EnsemblBacteria" id="ADZ09910">
        <property type="protein sequence ID" value="ADZ09910"/>
        <property type="gene ID" value="Metbo_1685"/>
    </dbReference>
    <dbReference type="GeneID" id="10278142"/>
    <dbReference type="KEGG" id="mel:Metbo_1685"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OMA" id="WFYSHKL"/>
    <dbReference type="OrthoDB" id="104789at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="281" mass="30797" checksum="179EC9E1E7B35CBD" modified="2011-05-03" version="1">MEKLKAIIQLIRPELPLAGGICVIAGQIIVLQNLPSLYVGLLGFLTGFFISSAAMVTNDYFDLEVDRVNHPQRPLPSGKISLSEIKVLTAILSILGFLTAALLGPLTLALSIFLWVVAIMYNWKCKETGLLGNVMVAISVTGFFIFGGLSVGGLINGIIWIFGALAFLFDLAEEIAGDAMDMEGDKERSSKTIARTHGKKYALTVSSTLYSFLVILSLVPLIMGWLDLKFLYIFIPLDMAIIYLALKILKTTTAEEGRKRIRQLFLTMTAFVIIFVALSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9N2</accession>
    <name>F0T9N2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645262.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1686"/>
    <dbReference type="EnsemblBacteria" id="ADZ09911">
        <property type="protein sequence ID" value="ADZ09911"/>
        <property type="gene ID" value="Metbo_1686"/>
    </dbReference>
    <dbReference type="GeneID" id="10278143"/>
    <dbReference type="KEGG" id="mel:Metbo_1686"/>
    <dbReference type="eggNOG" id="arCOG02740">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1085239_0_0_2"/>
    <dbReference type="OMA" id="ICRKVCP"/>
    <dbReference type="OrthoDB" id="83897at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="28089" checksum="0993450BDEFF519F" modified="2011-05-03" version="1">MKEKIIEKAKELGVDDIGIASIENYNSPNSVDVHELFPTVKNMVVLAFQQVDNMESNSSQFASVGIKLISDFSHSVSYQLANYIKTNFNAKVMIVPSSGPITMSKQTFMPSGFVSLRHAAYAAGLGNFGRHNLIIHPEMGSKVMFMAILTDLDLDEDSPLEETDCTYCDNCVKNCPVNALDEDGKTDVMKCLSNSQPYGFGGNKQFWTKFINSNPHEQEEMLSGDNYRLIYQAQALGSQYVCFNCTKTCPLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9U9</accession>
    <name>F0T9U9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645329.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1755"/>
    <dbReference type="EnsemblBacteria" id="ADZ09978">
        <property type="protein sequence ID" value="ADZ09978"/>
        <property type="gene ID" value="Metbo_1755"/>
    </dbReference>
    <dbReference type="GeneID" id="25394742"/>
    <dbReference type="KEGG" id="mel:Metbo_1755"/>
    <dbReference type="eggNOG" id="arCOG02276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_265168_0_0_2"/>
    <dbReference type="OMA" id="DYELMAT"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="145"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="200"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="433"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="545"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="619"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="671"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="746"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="798"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="929"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="1167"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1265" mass="145873" checksum="55B66EB46DB09856" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9W1</accession>
    <name>F0T9W1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0532</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644135.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0532"/>
    <dbReference type="EnsemblBacteria" id="ADZ08784">
        <property type="protein sequence ID" value="ADZ08784"/>
        <property type="gene ID" value="Metbo_0532"/>
    </dbReference>
    <dbReference type="GeneID" id="10276977"/>
    <dbReference type="KEGG" id="mel:Metbo_0532"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_2"/>
    <dbReference type="OMA" id="IVVYVYE"/>
    <dbReference type="OrthoDB" id="80065at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19411" checksum="B6EE325F2322160D" modified="2011-05-03" version="1">MLIGLISDTHIPERANKIPEKVLEVFKGVEMIMHAGDLVSRDVLEELEEVAPTICVQGNMDRMYGLKIPKREVITVENFTIGLDHGEVYPRGDTQQLKYIGMEMGVDVLVTGHTHTPFIKELENLVLLNPGSPTVPRMSDPTVMLVDVEKNDLNAQIIKLGDSTCKSLNYKGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z4</accession>
    <name>F0T9Z4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase</fullName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0566</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644168.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0566"/>
    <dbReference type="EnsemblBacteria" id="ADZ08817">
        <property type="protein sequence ID" value="ADZ08817"/>
        <property type="gene ID" value="Metbo_0566"/>
    </dbReference>
    <dbReference type="GeneID" id="10277011"/>
    <dbReference type="KEGG" id="mel:Metbo_0566"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_2"/>
    <dbReference type="OMA" id="IGVKFGC"/>
    <dbReference type="OrthoDB" id="7134at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="8"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="196"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="375"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="536" mass="58730" checksum="C6269ABA0D95F381" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA03</accession>
    <name>F0TA03_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase alpha subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644177.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0575"/>
    <dbReference type="EnsemblBacteria" id="ADZ08826">
        <property type="protein sequence ID" value="ADZ08826"/>
        <property type="gene ID" value="Metbo_0575"/>
    </dbReference>
    <dbReference type="GeneID" id="10277020"/>
    <dbReference type="KEGG" id="mel:Metbo_0575"/>
    <dbReference type="eggNOG" id="arCOG00096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078510_1_0_2"/>
    <dbReference type="OMA" id="PYIEEFC"/>
    <dbReference type="OrthoDB" id="73019at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012061">
        <property type="entry name" value="Glu_synth_lsu_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006519">
        <property type="entry name" value="GOGAT_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="1">
        <location>
            <begin position="14"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="24577" checksum="67656378A0757850" modified="2011-05-03" version="1">MSEFIINAENQPPREVNRDIRKAAVDYDSIVVKNPNAMHYIAAGLIEPVEIVIDGSAGYFAGTMIDGAKIHITENAGWFPGDNMTSGEVIIDGSAGDGVGQGIYGGTVTVKRDVGSRTGEIMKGGTIIVGGNSGFMTGLFMMGGRIIILGDVSQDAGESIIRGAIYVRGSVESLGKNAKIDELNNDDLTELKELLPEYGFDLSEKDYESFKKIVPRSNRPFYGKESEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA27</accession>
    <name>F0TA27_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">30S ribosomal protein S24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps24e</name>
        <name evidence="7" type="ordered locus">Metbo_0599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644201.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0599"/>
    <dbReference type="EnsemblBacteria" id="ADZ08850">
        <property type="protein sequence ID" value="ADZ08850"/>
        <property type="gene ID" value="Metbo_0599"/>
    </dbReference>
    <dbReference type="GeneID" id="10277044"/>
    <dbReference type="KEGG" id="mel:Metbo_0599"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="OMA" id="QYIRSYF"/>
    <dbReference type="OrthoDB" id="124805at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_S24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_S24e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="PTHR10496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="11428" checksum="7CBE807BE2AE8960" modified="2011-05-03" version="1">MEINVNEKIENPLLNRTEIHFDCTYQGEATPKIMDVKNRLIATLDVDKNLLVVHNLKPSYGEGKAIGYAKIYDSEESLAKIEKDHVVKKNAAVVEEAEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA38</accession>
    <name>F0TA38_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645339.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09988">
        <property type="protein sequence ID" value="ADZ09988"/>
        <property type="gene ID" value="Metbo_1765"/>
    </dbReference>
    <dbReference type="GeneID" id="10278222"/>
    <dbReference type="KEGG" id="mel:Metbo_1765"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OMA" id="HWIFWIS"/>
    <dbReference type="OrthoDB" id="42639at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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</entry>
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    <accession>F0TA66</accession>
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                <person name="Copeland A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Brauer S.L."/>
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    </evidence>
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</entry>
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                <person name="Lapidus A."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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</entry>
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">AL-21</strain>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GeneID" id="10278267"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="208" mass="25170" checksum="B7322D607E37F6D0" modified="2011-05-03" version="1">MEDLYFDVYSDMVEYVLDKFQVEVPNYKIMFLTDREFDVFNYECDIVRSFYYVITQEKQFGFNKYKNMYLDMVTKEYGEFFYQSEEDILASLIHVNKVYNTLEPVHNALQILTENDATNKDVSFLHNLITTNIDQDYLILLRKTRHENLSNGLIDDNNIFFDVVHEALHIIEHENQHSKWKFWTKKRMNSDEMDSITKEIFSEWNIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA95</accession>
    <name>F0TA95_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction histidine kinase</fullName>
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    </protein>
    <gene>
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    <organism evidence="4 5">
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
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                <person name="Brauer S.L."/>
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</entry>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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        </source>
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        <source>
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</entry>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="115" mass="13190" checksum="D4D8421DC336D493" modified="2011-05-03" version="1">MSVVKEPVSFGRMTKGEISDLIVPEVFIKDIHFSTPEHMMIMIRVTGPDSNKLKLYFEDSEDEIFQINTLMSENDEKISDNFILTDSYIDDGPSLSVDIDSEPETVRVILDFLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAI3</accession>
    <name>F0TAI3_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1833"/>
    <dbReference type="EnsemblBacteria" id="ADZ10055">
        <property type="protein sequence ID" value="ADZ10055"/>
        <property type="gene ID" value="Metbo_1833"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1833"/>
    <dbReference type="eggNOG" id="arCOG14010">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285073_0_0_2"/>
    <dbReference type="OMA" id="EHALIRP"/>
    <dbReference type="OrthoDB" id="127830at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11690" checksum="8D60EEF071C967CF" modified="2011-05-03" version="1">MSEDKDLSAIEEKADEILKGHNGVYLDNRLYSALRKDFPELSRKDFRKVIDNLLKTGYSIERGLIRPLTENEIKVQMKEHDEGKNSGKGSSDRPRLSTKRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAP9</accession>
    <name>F0TAP9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thermosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1901</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1901"/>
    <dbReference type="EnsemblBacteria" id="ADZ10121">
        <property type="protein sequence ID" value="ADZ10121"/>
        <property type="gene ID" value="Metbo_1901"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1901"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="OrthoDB" id="11742at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="PTHR11353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="579" mass="62328" checksum="3423A47B2177FD75" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAQ3</accession>
    <name>F0TAQ3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="9">HTPA reductase</shortName>
            <ecNumber evidence="9">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapB</name>
        <name evidence="12" type="ordered locus">Metbo_1905</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645476.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1905"/>
    <dbReference type="EnsemblBacteria" id="ADZ10125">
        <property type="protein sequence ID" value="ADZ10125"/>
        <property type="gene ID" value="Metbo_1905"/>
    </dbReference>
    <dbReference type="GeneID" id="10278363"/>
    <dbReference type="KEGG" id="mel:Metbo_1905"/>
    <dbReference type="eggNOG" id="arCOG04393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_2"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="OrthoDB" id="86833at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="10">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DapB_C" evidence="11">
        <location>
            <begin position="134"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="(S)-2,3,4,5-tetrahydrodipicolinate binding" evidence="9">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="273" mass="29063" checksum="B7D1498BCD8C1FBA" modified="2011-05-03" version="1">MIKVAVTGACGRMGSKILKKILEQDDMEVVAAIESPNTPFQSKDVGEIIGIGELGVEVNGAEKLAETLKTSKADVLVDFTIADAAVGTIKTTSKEGVNLVVGTTGFSDQQLKEIKESIHKNQVSAVIAPNMAVGVNVFFKIIKDVAALIGDYDVEIIEAHHRHKKDSPSGTAVRAAEIIAEELNRNLDEVGVYGRKGIVGERTDEEIGIHAVRGGDIVGDHTVLFAGEGERIEIVHRAHSRQSFVSGVIKALRYVGTAQKGSISDMGDVLGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR2</accession>
    <name>F0TAR2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0687"/>
    <dbReference type="EnsemblBacteria" id="ADZ08938">
        <property type="protein sequence ID" value="ADZ08938"/>
        <property type="gene ID" value="Metbo_0687"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0687"/>
    <dbReference type="eggNOG" id="arCOG04903">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567127_0_0_2"/>
    <dbReference type="OMA" id="GCARTNE"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012356">
        <property type="entry name" value="UCP018781_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09885">
        <property type="entry name" value="DUF2112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018781">
        <property type="entry name" value="UCP018781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03271">
        <property type="entry name" value="methan_mark_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="161" mass="17991" checksum="68551799E4AB06B5" modified="2011-05-03" version="1">MNLIIQRGNFIKIAIFPPNSLILADLVERKGHEALVLQKEIRKRVTDVEIDSPPLNITEEEPIKGLKYAAIEVPSGVRGRMAIFGPLIEAAEAAIIMEDAPFGFGCIGCARTNELSMFYLRKRGIPLLELHYPTTKEETMEVVNKINTFLDSLEPLEDKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT4</accession>
    <name>F0TAT4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0709</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644311.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0709"/>
    <dbReference type="EnsemblBacteria" id="ADZ08960">
        <property type="protein sequence ID" value="ADZ08960"/>
        <property type="gene ID" value="Metbo_0709"/>
    </dbReference>
    <dbReference type="GeneID" id="10277154"/>
    <dbReference type="KEGG" id="mel:Metbo_0709"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_2"/>
    <dbReference type="OMA" id="GYDITRT"/>
    <dbReference type="OrthoDB" id="40216at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="327" mass="34042" checksum="452B30C7404E5EEC" modified="2011-05-03" version="1">MDWLLIIGVITAMYMAFNIAANDIGNSMGTVVGSGALTMRKALIVGALFEFLGAMFLGSSVIKTVGSGIVPLEFMTGLGAFIITMSAGIWITITLIKKIPISGSDAIVSSVLGYGLVYAGVNHLNWGTIGFIMASWVISPLIGLASGFLVYYLLKLGILNRIKSNIGAKDRAEKVFSYLQIGSSSFAALGVGAIDIAAATAVLYVTVGATIGFSIKFLGAIALVFGILIAGNRITDTIGRRITELVPTRGFSAQISAGSITLLFASIGMPISPTQTLVGSVIGVGMARGTSTVKLDVIKHIATTWVITIPACIAISATLYFVINAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU4</accession>
    <name>F0TAU4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Metbo_0719</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">AL-21</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
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            <topology evidence="3">Multi-pass membrane protein</topology>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU5</accession>
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                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
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            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="ADZ08971.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="576" mass="66348" checksum="6EF1AB6E1218CB16" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV5</accession>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
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                <person name="Pitluck S."/>
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                <person name="Kyrpides N."/>
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                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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    </comment>
    <dbReference type="EMBL" id="CP002551">
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    <dbReference type="OMA" id="AEMILIV"/>
    <dbReference type="Proteomes" id="UP000007490">
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    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
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    <dbReference type="Pfam" id="PF04021">
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="55" mass="5889" checksum="8295A2521A1D41EC" modified="2011-05-03" version="1">MDEKGQISAEMILIMGVLLVIVIVAGAFITNMAHQIADSVNSVLNTARSSTINRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV7</accession>
    <name>F0TAV7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644334.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08983">
        <property type="protein sequence ID" value="ADZ08983"/>
        <property type="gene ID" value="Metbo_0732"/>
    </dbReference>
    <dbReference type="GeneID" id="10277179"/>
    <dbReference type="KEGG" id="mel:Metbo_0732"/>
    <dbReference type="eggNOG" id="arCOG10264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514638_0_0_2"/>
    <dbReference type="OMA" id="ITYFEIR"/>
    <dbReference type="OrthoDB" id="81899at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="187" mass="22117" checksum="1A46E74E2A3B8D48" modified="2011-05-03" version="1">MPKNYNMKETILELLNGRELSKKELLEEIRRESERSTSDKTLNESLMTLLREKRIYITNYDFSIYNDVKRIQSIKPEGIVFGLMKTDFVEIETLIKLLESNDVDEVKLASSKLKMNFRNKMDELNFRVSSIKNEDLDALFNRIIFYIYSQSEDQKRILINKLAWSLSNENGSLDLFEDLLNFIDHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAY7</accession>
    <name>F0TAY7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiD</name>
        <name evidence="6" type="ordered locus">Metbo_1913</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645484.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1913"/>
    <dbReference type="EnsemblBacteria" id="ADZ10133">
        <property type="protein sequence ID" value="ADZ10133"/>
        <property type="gene ID" value="Metbo_1913"/>
    </dbReference>
    <dbReference type="GeneID" id="10278371"/>
    <dbReference type="KEGG" id="mel:Metbo_1913"/>
    <dbReference type="eggNOG" id="arCOG04383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="OMA" id="YHGKLIK"/>
    <dbReference type="OrthoDB" id="57676at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="358" mass="38526" checksum="08D2E09E89AE9533" modified="2011-05-03" version="1">MENRAILDRYHLKESEYGITTGSAATAAALAALFSLNGEVKEVKINTPLGEVILDVKHSEKLNSCSGRASVVKYPYDDPDVTINLEVVAELILKNEPGITVKGGDGVGTVTKPGLQVPVGCPAINPTPMEMIVSNLESHLPEGKGAEVTIIVPHGKELAKRTLNPRLGVVGGISILGTTGFARSMNLKSYKESYRCQIDVAVAGGYEYLVFVPGNIGEKIAKQLLDIEEDQIVQMGNFVGYMLDEASKKGVKQIMLLGHAGKLIKISAGIFNTKHSVADGRHEIIASHAALQGADQQVVGQLFKSTTTEDMITVLEENGLKTKVFNSIAKKMKQLCSSKFNMDFEVTIVNMEGEILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB04</accession>
    <name>F0TB04_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein MarR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645501.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1930"/>
    <dbReference type="EnsemblBacteria" id="ADZ10150">
        <property type="protein sequence ID" value="ADZ10150"/>
        <property type="gene ID" value="Metbo_1930"/>
    </dbReference>
    <dbReference type="GeneID" id="10278388"/>
    <dbReference type="KEGG" id="mel:Metbo_1930"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_11_0_2"/>
    <dbReference type="OMA" id="KIHEELH"/>
    <dbReference type="OrthoDB" id="94835at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="18601" checksum="E34B4BC914F40909" modified="2011-05-03" version="1">MDDQLAVTRKMMMQFIRMNKKFQKFEKNPIEFGDGQKLYPSEIHILDAVGSGVANNVTVLSQKFGITKGAVSQVVNKLHEKQLIKKEKIEGNAKEVKLTLTSHGRNAFKIQDELHKNFENEFFNYFKNLEQDKVDSYLEIMDTIDLFMDKVLNDQKPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB67</accession>
    <name>F0TB67_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0767</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644369.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09018">
        <property type="protein sequence ID" value="ADZ09018"/>
        <property type="gene ID" value="Metbo_0767"/>
    </dbReference>
    <dbReference type="GeneID" id="10277215"/>
    <dbReference type="KEGG" id="mel:Metbo_0767"/>
    <dbReference type="eggNOG" id="arCOG01964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_6_1_2"/>
    <dbReference type="OMA" id="TYEDSLW"/>
    <dbReference type="OrthoDB" id="119119at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="10">
        <location>
            <begin position="142"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="238"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ09018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="283" mass="32732" checksum="6423C8031EB06632" modified="2011-05-03" version="1">MNEINKRPGIIWQSILVFLIVLDGILLFLTLISPNLKSTTIANLGIFDLAVSILLLFVFLWRMKQHQHEYPQFIKNNWKDILAFIPIYFISYYIFGLHDIGIIMKIIVVIKLAALYIFSKKVGKEFIKYQEKTRLVYALAFFLVVFFFCSYIFYLVEHGVNPEVSTFEDSIWFVLQTITTVGYGDIIPVTGIGRLMGVISMFSALVLTSIITSVATFSLIEKFRKGTEALTEKTGKYVETMDEKLDHLNHRLDELEKIDEISKNLEEMKSEIEALKQMIKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB85</accession>
    <name>F0TB85_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14</name>
        <name evidence="5" type="ordered locus">Metbo_0785</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644387.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0785"/>
    <dbReference type="EnsemblBacteria" id="ADZ09036">
        <property type="protein sequence ID" value="ADZ09036"/>
        <property type="gene ID" value="Metbo_0785"/>
    </dbReference>
    <dbReference type="GeneID" id="10277233"/>
    <dbReference type="KEGG" id="mel:Metbo_0785"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="91632at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_L14P_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="132" mass="14127" checksum="EC9B118D85398B4F" modified="2011-05-03" version="1">MKAITSNVTKALPIGARLQCVDNTGAREVEIISVKGYKGVRRRLAPAGVGDMIVISVKKGTVDMRKEVTTAVVVRQKKEYKRADGLRIKFEDNAAVIVSPEGVLKGSEIRGPIAKEAADRWPAIGSAASIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD4</accession>
    <name>F0TBD4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_1983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645554.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1983"/>
    <dbReference type="EnsemblBacteria" id="ADZ10203">
        <property type="protein sequence ID" value="ADZ10203"/>
        <property type="gene ID" value="Metbo_1983"/>
    </dbReference>
    <dbReference type="GeneID" id="10278443"/>
    <dbReference type="KEGG" id="mel:Metbo_1983"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_3_2"/>
    <dbReference type="OMA" id="GNWPPAW"/>
    <dbReference type="OrthoDB" id="90547at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="189"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="476" mass="53946" checksum="77B6BA5BE4F5C801" modified="2011-05-03" version="1">MDVLLINPYDENAVKNGLGFITPPLNLMYLAASLEKNNHSVEIFDDDLMQKGFFEVRDHVEKLDPEIIGITATTSTIKTALKYLEEIKKVMPHVLTVIGGPHTTFLPVNTLQNSDDLDVVVLGEGEETFADLANNQVDGSGSLEDVKGIVYRDPETGSIKTNNPRPLIEDLDSLPFPARHLVSFESYGISKSQSGGMITSRGCVYNCGYCSSSLIMGKKFRSRSPENVVDEIEELVYNYHLNDIAFMDDTFMLNKNRAASIADEIKERNLDVSFVASSRVDMVNQDLLFKLKNAGMNTIYYGVESGSQRILNLMKKGINLKQAENAVKNAKNAGLEVLTSYIIGYPGETQNDMNETIKFSIKLEPDYCQYSILTPFPGTPIYHDLKDKDLIKTENWEKYTVMKPILNYEKLGLNKDMIERNLARAYLKFYTRPKYLLKHRYMLKVMIETLFRSFIIPKFRGSTVKGWYQNLDNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBI5</accession>
    <name>F0TBI5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645605.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2035"/>
    <dbReference type="EnsemblBacteria" id="ADZ10254">
        <property type="protein sequence ID" value="ADZ10254"/>
        <property type="gene ID" value="Metbo_2035"/>
    </dbReference>
    <dbReference type="GeneID" id="10278496"/>
    <dbReference type="KEGG" id="mel:Metbo_2035"/>
    <dbReference type="eggNOG" id="arCOG10246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145243_0_0_2"/>
    <dbReference type="OMA" id="DRFHDSH"/>
    <dbReference type="OrthoDB" id="86801at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="249" mass="28713" checksum="5D6493AC90B272F7" modified="2011-05-03" version="1">MENFKVNRGSDNRTFIETRSYKNLHKQLKTLKTRHGRIIHVVGAPGTGKSSNIYAAIKELGLNCYDLELGLMDPNADASKLMDQIYHDLKSGLNARSKSEIYHDLASFDALVIADKFHDTHLMNTEMVGYSLWTKTKGIGSLKFYILCMKEYLLNRKKYEEINIIIQTAWTVKLNKEKKDLFTDFGILSRFALAMLRLFFEVVEIRYSREETVEIVKSHVDADQKIIEAHIDELGNKPRLVCQAIKYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM9</accession>
    <name>F0TBM9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644457.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0856"/>
    <dbReference type="EnsemblBacteria" id="ADZ09106">
        <property type="protein sequence ID" value="ADZ09106"/>
        <property type="gene ID" value="Metbo_0856"/>
    </dbReference>
    <dbReference type="GeneID" id="10277305"/>
    <dbReference type="KEGG" id="mel:Metbo_0856"/>
    <dbReference type="eggNOG" id="arCOG01385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_878859_0_0_2"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="298" mass="34634" checksum="891C42603CC779ED" modified="2011-05-03" version="1">MCCKVSIILPTYNRGFIIGDCLKSVITQTYDNWELIIADDGSNDNTEDIIKGLSKNNQKIIYYKNSQNLGLPGNRNKAIKEASGHVVFFIEDDMVLRADCLENLVKTYELLSKQGEKIGAICPSLITQISYNTSQRGVLNHVRDSRNDDLEKSPCIIDKKTGLIYRNFSPKFNGIIIVEDCHSCSLYPKEIFEKIQYPENIYTGNYIGEESEMNIRLRKMGYNLYFQPKAVMNHFVQENGGCRLPLYKWSYYFIRNHSLFLARNYSYKFVYMESFFLFYILRTVVSFSFLSLNKQPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBN6</accession>
    <name>F0TBN6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase/dehydrogenase beta subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0863</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644464.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0863"/>
    <dbReference type="EnsemblBacteria" id="ADZ09113">
        <property type="protein sequence ID" value="ADZ09113"/>
        <property type="gene ID" value="Metbo_0863"/>
    </dbReference>
    <dbReference type="GeneID" id="10277312"/>
    <dbReference type="KEGG" id="mel:Metbo_0863"/>
    <dbReference type="eggNOG" id="arCOG02473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_3_0_2"/>
    <dbReference type="OMA" id="PANWYRA"/>
    <dbReference type="OrthoDB" id="19205at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="426" mass="48637" checksum="FF3D0DD4DEF19FE4" modified="2011-05-03" version="1">MIINNILEVSKKGLCVGCGTCESVCPKNSIKMVINKKKGIYLPNINQKLCNDCELCIKSCPGYELDFNSLSSELFNKCNTNQLIGKFLDTFIGYSTNMHIRYNSSSGGMITQILISSLELGIIDGALVTRMKKGNPLIPEPFIAKTKEEIIEASKSKYCPVPLNSALNELMTTKLKKIAVVGLPCHIHGIRKAEKINNKLRKKIIIHLGIFCIYTPTFNGTKLLLKKLSLNEEKIKNIEYRGKGWPGSMKIIDHKHEIIVNEYWKFIGLNFFIPFRCLKCCDGTNELADISFGDAWLPEYSNDKIGSSIIISRTKFSLELLYSLNENNKINLNEIDVGKVIESQRGMLYFKKKSFESRIKISRNSPEYNIKHLNPYLMDYVTSIYIYLSIYLASFSIFRKLLPHIPDKILVLYKLFPTLINRKKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBQ5</accession>
    <name>F0TBQ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644483.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0883"/>
    <dbReference type="EnsemblBacteria" id="ADZ09132">
        <property type="protein sequence ID" value="ADZ09132"/>
        <property type="gene ID" value="Metbo_0883"/>
    </dbReference>
    <dbReference type="GeneID" id="10277332"/>
    <dbReference type="KEGG" id="mel:Metbo_0883"/>
    <dbReference type="eggNOG" id="arCOG06462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091578_0_0_2"/>
    <dbReference type="OMA" id="ECLFIVP"/>
    <dbReference type="OrthoDB" id="93501at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="242" mass="27940" checksum="D4AEF38CBEDFB49A" modified="2011-05-03" version="1">MITVICVYNNRVLLEDRLLKSLKIQNTEYELILLDNTMGKYESAARALNCGAKQATKDYLMFVHQDVDLLYEDCLKDIEESLRELDDLGVAGVAGYTETNGKFIMYSNIKDGRPPEDVGVYLKDPLKVQTVDECLFIVPRSIFEKLKFDEITCPGWHLYGVDYCLSTTEFGKSVYVVPINIYHVSRTESFSKDYYTLLKNIVRKHGKKYKTIDTSCGIWNTNSVRLDINILEDKLLRFLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBT7</accession>
    <name>F0TBT7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ordered locus">Metbo_2060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645630.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2060"/>
    <dbReference type="EnsemblBacteria" id="ADZ10279">
        <property type="protein sequence ID" value="ADZ10279"/>
        <property type="gene ID" value="Metbo_2060"/>
    </dbReference>
    <dbReference type="GeneID" id="10278522"/>
    <dbReference type="KEGG" id="mel:Metbo_2060"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="OrthoDB" id="90096at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="Transl-rel_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PTHR10853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="SSF159065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1_1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADZ10279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="39374" checksum="6430A102E80AC557" modified="2011-05-03" version="1">MRIVYQDKKKGVIEVVPETLDDLWHLSHIIEKGDLVSSLTSRRIQDSTGERIRSDRGIKKTFFMGVRVDDISFHKYTGKLRATGTIEVGPEDLVPLGSFHTLDLKLKNSIKIQKEHWSKWNLKRLKDSIKSSKKPTALIVAIEDDVADIGIIRQYGVDYYGPIIGSVSGKRIVQKNRKQAINDFFTELAEVIKGFKEVNTVILAGPGFSKGDFHDFLVQKYAEIAKMTVLESTGAGGRTGITEVLKKGVIEKMATEGRIAFEIRKVEELLSEIGKSSKLVAYGKKEVGEAVQTGAAETLLVIDEMVRERDIEKLMDLTENMGGNVVIISSEHDGGKQLSALGGIAATLRYPLTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ5</accession>
    <name>F0TBZ5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="2">4.2.1.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.75" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645688.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2118"/>
    <dbReference type="EnsemblBacteria" id="ADZ10337">
        <property type="protein sequence ID" value="ADZ10337"/>
        <property type="gene ID" value="Metbo_2118"/>
    </dbReference>
    <dbReference type="GeneID" id="10278581"/>
    <dbReference type="KEGG" id="mel:Metbo_2118"/>
    <dbReference type="eggNOG" id="arCOG02048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_9_5_2"/>
    <dbReference type="OMA" id="VWAVGKT"/>
    <dbReference type="OrthoDB" id="77713at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40082">
        <property type="entry name" value="PTHR40082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="1">
        <location>
            <begin position="24"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="261" mass="28758" checksum="1BAEDA0B59AF7C7D" modified="2011-05-03" version="1">MKGKEFEGKVIGITRPEERVKEAVAIVEEHGGTALVAPTLELQVSNTQSLIQLCEMAGELDWLIFTSPTGIISVFKHCSDLKTRLNPNCKIAVIGPRTENYLEKKGLKADIVPESYTAEGLLECFEDHDLKNKKIGIPRTLAARDALPTGLKVMGADVFVAEAYKSDLPKDRTRVNQLVEAILNRKINALTFTSTLTVKNLFDMLDETEKQDVVDILKSDDVVVAAIGPVTAMPLHELDIDVLIPEKYTVSAMLEILMGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC47</accession>
    <name>F0TC47_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644549.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0951"/>
    <dbReference type="EnsemblBacteria" id="ADZ09198">
        <property type="protein sequence ID" value="ADZ09198"/>
        <property type="gene ID" value="Metbo_0951"/>
    </dbReference>
    <dbReference type="GeneID" id="10277400"/>
    <dbReference type="KEGG" id="mel:Metbo_0951"/>
    <dbReference type="eggNOG" id="arCOG04214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="OMA" id="VRHTSCS"/>
    <dbReference type="OrthoDB" id="85388at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="14829" checksum="BCF074DAA01EBABF" modified="2011-05-03" version="1">MYKRVFEVKTSDRLEIIDISSGINSIVAGSGVKTGLVNLFSQHSTSGIVINENESGLVEDFKNLLKKIVQDESGYKHDRIDNNADSHLRSLLMGNSVNVPVENGRMELGTWQSIFFIELDGPRTRNIIVTVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC83</accession>
    <name>F0TC83_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644585.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0987"/>
    <dbReference type="EnsemblBacteria" id="ADZ09234">
        <property type="protein sequence ID" value="ADZ09234"/>
        <property type="gene ID" value="Metbo_0987"/>
    </dbReference>
    <dbReference type="GeneID" id="10277436"/>
    <dbReference type="KEGG" id="mel:Metbo_0987"/>
    <dbReference type="eggNOG" id="arCOG03165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087537_0_0_2"/>
    <dbReference type="OMA" id="RENLHES"/>
    <dbReference type="OrthoDB" id="94554at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="264" mass="30983" checksum="7710F6F91D599FA0" modified="2011-05-03" version="1">MKILERFSWNIKIAGILIFSSLVLYIVQTMIFREPKTELFYIGINMVFLPIEALIVVIIIQTGINEREKKLMLEKLNMVIGAFFSEVGTELLTEISKFDSKSTKTSKILIINSRWEDEDFEHAKKNINSLAHSLMIIPSNKEVADFLYQSKLFLQDKRMFLLALLENPNLLEHETFTDLLRAVFHLTEELDKRENTYDLPASDCEHLKEDVQRVYDLLIFEWLNYMEHLKNNYRYLFSLAMRTNPFDPNKTVVIKTVESSSNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC92</accession>
    <name>F0TC92_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645710.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2142"/>
    <dbReference type="EnsemblBacteria" id="ADZ10359">
        <property type="protein sequence ID" value="ADZ10359"/>
        <property type="gene ID" value="Metbo_2142"/>
    </dbReference>
    <dbReference type="GeneID" id="10278605"/>
    <dbReference type="KEGG" id="mel:Metbo_2142"/>
    <dbReference type="eggNOG" id="arCOG04361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097790_0_0_2"/>
    <dbReference type="OMA" id="HAVGINM"/>
    <dbReference type="OrthoDB" id="113690at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019271">
        <property type="entry name" value="DUF2284_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10050">
        <property type="entry name" value="DUF2284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="216" mass="24082" checksum="0F09D2B935493AF3" modified="2011-05-03" version="1">MVQEIAEFSENVSDDKLEADVEKYLVLAKELGANDAIRISTDMIIVDERVRAKCYSPRCPYYGTNLNCPPHFDWDLEKTIKIISKYKYGIFVMLQVPPEEQTGKDYDNIKHHIPGARKMYEIVAKVQSAAFYDGYPLALGFAGGPSCKRVFCSTVECKGIKGEGCRMALKSNPTMHSAGMDAYSMAKKVGWKIYPIGKTTEPSQIAHGLELGLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCG1</accession>
    <name>F0TCG1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin biosynthesis MoaE protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644589.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0991"/>
    <dbReference type="EnsemblBacteria" id="ADZ09238">
        <property type="protein sequence ID" value="ADZ09238"/>
        <property type="gene ID" value="Metbo_0991"/>
    </dbReference>
    <dbReference type="GeneID" id="10277440"/>
    <dbReference type="KEGG" id="mel:Metbo_0991"/>
    <dbReference type="eggNOG" id="arCOG00534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_2_2_2"/>
    <dbReference type="OMA" id="FEISVIH"/>
    <dbReference type="OrthoDB" id="97533at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="145" mass="16426" checksum="C01F77280F201D4C" modified="2011-05-03" version="1">MTVRIIEKGVEEISICSLLEELKKNPEINKCGAIFSFEGIVRGEETGKKTVQMDLTTPNQLETEQELKEIVYDVKQKYGVLEIAVVHYIGNFKPGDPLFLTAVSGSHRDETRKALHEIIERVKYELDFKKEEHTEDGTNIIMSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCK6</accession>
    <name>F0TCK6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644634.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1036"/>
    <dbReference type="EnsemblBacteria" id="ADZ09283">
        <property type="protein sequence ID" value="ADZ09283"/>
        <property type="gene ID" value="Metbo_1036"/>
    </dbReference>
    <dbReference type="GeneID" id="10277485"/>
    <dbReference type="KEGG" id="mel:Metbo_1036"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_2_2"/>
    <dbReference type="OMA" id="RPSIVDY"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="136"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="218" mass="23852" checksum="E7B04881FCE1E78B" modified="2011-05-03" version="1">MYVIIMGGGRLGLKLAKELIFDGHDLTLIEKDETKCNFLSSEIDELVLCGNATDAITLKNAEISVADAFVAATGNDETNLLAALMAKKLGAKKIIARLNEPQHEPIFVSNDFITVVVPETIEAGYLEKLVLKPKVADLFIVDHGNGELLELEVNNSKIIGKTVGELNSQDDFYICGIYDKKKSEVNIVNDSTLITKNCRLIVLTKKEAVGNVLKLFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCM7</accession>
    <name>F0TCM7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644655.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1057"/>
    <dbReference type="EnsemblBacteria" id="ADZ09304">
        <property type="protein sequence ID" value="ADZ09304"/>
        <property type="gene ID" value="Metbo_1057"/>
    </dbReference>
    <dbReference type="GeneID" id="10277506"/>
    <dbReference type="KEGG" id="mel:Metbo_1057"/>
    <dbReference type="eggNOG" id="arCOG01662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1227663_0_0_2"/>
    <dbReference type="OMA" id="FHNDIYL"/>
    <dbReference type="OrthoDB" id="99264at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="225" mass="24656" checksum="FCA284F3A3E20178" modified="2011-05-03" version="1">MKQNKNSLEIEEVIIEGYGCQVPVLKLTPPESRGSVVVAHNYGGSKEEMLGLAFRIAKTGFTTGVIDLRGHGENSCKMDGKILSDLETAITYFKQFGKVTAVGHSLGGRLSLISDADYAIGISPALAKTFGPKTQGMIRVLRDYRVNGSVTDLFTTLGKIPLIEFDPENVLIIRGSRDVPEIIAECDKLTSAGYEVVEIDDALHVDIYTLEPTFEAVTKKLQKWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ4</accession>
    <name>F0TCQ4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine--tRNA ligase</fullName>
            <ecNumber evidence="6">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
            <shortName evidence="6">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">serS</name>
        <name evidence="9" type="ordered locus">Metbo_2230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645795.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2230"/>
    <dbReference type="EnsemblBacteria" id="ADZ10444">
        <property type="protein sequence ID" value="ADZ10444"/>
        <property type="gene ID" value="Metbo_2230"/>
    </dbReference>
    <dbReference type="GeneID" id="10278696"/>
    <dbReference type="KEGG" id="mel:Metbo_2230"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_2"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="16385at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="PTHR11778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="178"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="6">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="45"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="262"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="349"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="426" mass="49100" checksum="26DA814360D12F50" modified="2011-05-03" version="1">MLDIKLFRENPDLILESEKKRFRTTENVEKVIEFDLKWRNGLKKLNELRAERNKLSKSFNEARKQGKDELKALQNRAKEISKEINELEPKISEYIEARDEFRYKVGNILDSDVPVSETEEDNKVISEFGDMPSFSFEPANHVDLIETIDGAEFKRAAEVSGSRFYYLKEDIVYLNMALLKFAMDTLSQKGYEIFQTPFFIKHDVIKETAELADFEETLYKIEGEDLFMIATSEQTLAALHRGEIIDERTLPRKYCGLSTCFRREAGSHGRDTLGIFRVHQFEKIEQFIFCDAESSRDLHQELLENAELIYQKLGLPYRIVSIVSGELNDNAAKKYDLEAWFPGSSTYRELVSCTNCGDYQARKLNARYGIHGDSDSLKMCHTLNSTAIATERTICCILENYQQEDGTIKVPEVLIPYMNGKTVLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCR0</accession>
    <name>F0TCR0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645801.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2236"/>
    <dbReference type="EnsemblBacteria" id="ADZ10450">
        <property type="protein sequence ID" value="ADZ10450"/>
        <property type="gene ID" value="Metbo_2236"/>
    </dbReference>
    <dbReference type="GeneID" id="10278702"/>
    <dbReference type="KEGG" id="mel:Metbo_2236"/>
    <dbReference type="eggNOG" id="arCOG14018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102097_0_0_2"/>
    <dbReference type="OMA" id="ETFFPRE"/>
    <dbReference type="OrthoDB" id="81744at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025063">
        <property type="entry name" value="DUF4004"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13171">
        <property type="entry name" value="DUF4004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="207" mass="24440" checksum="0E0252F0F18E5686" modified="2011-05-03" version="1">MSQDLISKKELLEITGISYGQLYRWKRKKLIPEEWFIKKSTFTGQETFFPKYKVIDRVEKIKNMKGDISLDSLADMLSPDLTEKIVSEKELKDRNIVSEATLEFTREEYGETKEFRFEQIISIYLLDKMFKSGKIGHSEGKIVFQLFSDKYQKFKGKNCELIFFRKMGVSSCCMVPDSKDVHFEETIKIMEIMNISELIEELKIKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS3</accession>
    <name>F0TCS3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spt5</name>
        <name evidence="6" type="ordered locus">Metbo_2249</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645814.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2249"/>
    <dbReference type="EnsemblBacteria" id="ADZ10463">
        <property type="protein sequence ID" value="ADZ10463"/>
        <property type="gene ID" value="Metbo_2249"/>
    </dbReference>
    <dbReference type="GeneID" id="10278715"/>
    <dbReference type="KEGG" id="mel:Metbo_2249"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="OMA" id="FSEIEHF"/>
    <dbReference type="OrthoDB" id="91936at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NGN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ10463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="147" mass="16185" checksum="5DCE32F579F56FBB" modified="2011-05-03" version="1">MIYAIRTLVGQEKNVARIIGRNVKNSGIEVHSILVPESLRGYILVESSTKIDMQNPAFKVPHMKGAIEGEIPYEEVKSFLNPEPILASVQKGSIVELISGPFKGEKAKVVRIDESKEDVVLELIEAAVPIPVTVKGDQIRLIQKEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCT2</accession>
    <name>F0TCT2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645823.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2258"/>
    <dbReference type="EnsemblBacteria" id="ADZ10472">
        <property type="protein sequence ID" value="ADZ10472"/>
        <property type="gene ID" value="Metbo_2258"/>
    </dbReference>
    <dbReference type="GeneID" id="10278724"/>
    <dbReference type="KEGG" id="mel:Metbo_2258"/>
    <dbReference type="eggNOG" id="arCOG10855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712885_0_0_2"/>
    <dbReference type="OMA" id="FNAGANE"/>
    <dbReference type="OrthoDB" id="122345at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="71" mass="8485" checksum="8CB131D621BBD93A" modified="2011-05-03" version="1">MEMKEMEIQIFEDLKGDYDGVEKITMQSEQEFLVFADDETLWNIFEDMINEFSSIELDAEKGETHFLRIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S4</accession>
    <name>F0T5S4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644668.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1072"/>
    <dbReference type="EnsemblBacteria" id="ADZ09317">
        <property type="protein sequence ID" value="ADZ09317"/>
        <property type="gene ID" value="Metbo_1072"/>
    </dbReference>
    <dbReference type="GeneID" id="10277521"/>
    <dbReference type="KEGG" id="mel:Metbo_1072"/>
    <dbReference type="eggNOG" id="arCOG12964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053973_0_0_2"/>
    <dbReference type="OMA" id="NYFEGWY"/>
    <dbReference type="OrthoDB" id="36436at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009976">
        <property type="term" value="F:tocopherol cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025893">
        <property type="entry name" value="Tocopherol_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309">
        <property type="entry name" value="PTHR35309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14249">
        <property type="entry name" value="Tocopherol_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="327" mass="37701" checksum="13DF659681BCC98D" modified="2011-05-03" version="1">MFKIWNPEIFQGQNKKKEYFEGWYFKSVSKDEETAYAIIVGVSITKISENSHAFIMLMDARNQVLHYFSYPMSSFWANKDKFEIKIANNYFSLDHMILNIDDGGKRVKADMEFENIVPWPVTRFSPGAMGFFAFIPFLECYHGVLSFNHTIKGYVTINNDKIDFTDGKGYIEKDWGSSMPSSWIWMQTNHFDKEGISLFVSIAKIPWLGSYFTGYIFGLVYDGKIYKFTTYNRAKIEKLDVTDADIEIKISDKNYCLQINALRAEGVDLPAPKLGEMTSKVNESLRSKIHIKLYKKEKNTNKLLYQGTGKNAGLEFVGNLDELLKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5S9</accession>
    <name>F0T5S9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymorphic membrane protein Chlamydia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_1077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09322">
        <property type="protein sequence ID" value="ADZ09322"/>
        <property type="gene ID" value="Metbo_1077"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1077"/>
    <dbReference type="eggNOG" id="arCOG02555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013416_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003368">
        <property type="entry name" value="POMP_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01376">
        <property type="entry name" value="POMP_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Big_5" evidence="4">
        <location>
            <begin position="456"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ09322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="560" mass="59247" checksum="32BE3895D8E8D4EB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U3</accession>
    <name>F0T5U3_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_1091</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644687.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1091"/>
    <dbReference type="EnsemblBacteria" id="ADZ09336">
        <property type="protein sequence ID" value="ADZ09336"/>
        <property type="gene ID" value="Metbo_1091"/>
    </dbReference>
    <dbReference type="GeneID" id="10277540"/>
    <dbReference type="KEGG" id="mel:Metbo_1091"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514647_0_0_2"/>
    <dbReference type="OMA" id="TCPYANT"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ09336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="201" mass="22829" checksum="74AA700EDBA845A9" modified="2011-05-03" version="1">MKYRCDICGYIYNPETGDDENDIKAGIDFYDLPSDWVCPRCGIDREQFIPVDKLTGSEGEGPMALMILALTNGLWNISGRGSYSVTREIGRVFVNELKKEGVNFKNSKDSLECVKDYFLRNKFAGGVLYDINEEGAELEIKNCRFFGICKQLENQGVLITTCPYTNTAAMALEESTGYRYRIKKEQEGYGHHIELKRVSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5U4</accession>
    <name>F0T5U4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyamine-transporting ATPase</fullName>
            <ecNumber evidence="9">3.6.3.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_1092</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.3.31" evidence="9"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644688.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1092"/>
    <dbReference type="EnsemblBacteria" id="ADZ09337">
        <property type="protein sequence ID" value="ADZ09337"/>
        <property type="gene ID" value="Metbo_1092"/>
    </dbReference>
    <dbReference type="GeneID" id="10277541"/>
    <dbReference type="KEGG" id="mel:Metbo_1092"/>
    <dbReference type="eggNOG" id="arCOG00177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OMA" id="MVMEPKV"/>
    <dbReference type="OrthoDB" id="88924at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Mop" evidence="8">
        <location>
            <begin position="285"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="348" mass="39480" checksum="DDF274BA84084FF7" modified="2011-05-03" version="1">MIKIEHLSKDWKEFKINDVTLEINEDEYFVFLGPSGSGKTMLLELIAGMWTPDSGKIYINNQDVTNFPPEKRGVGFVYQNYMLFPHKTVFENIAFGLNIRKVDKKKIEEQVGEMMDLFGISHLAHRLPRTLSGGEQQRTALARALIIRPKVLLMDEPLSALDRITREELIREFKKIHEEFNITIIQVTHNFDEALQLAERIAIIKQGTVSQVGSTDEVFRRPKDEFVADFVGTENIIRGVATDGEENLTSVDTGNIVVESTEHKTGKVHFTIRPEAITVSTSRITTSARNEFKGKLTEIYDLGTIIKLTVDVGEQFVLVLTRQSFDDLELNIGKEVFISFKATAVNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y1</accession>
    <name>F0T5Y1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644725.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1130"/>
    <dbReference type="EnsemblBacteria" id="ADZ09374">
        <property type="protein sequence ID" value="ADZ09374"/>
        <property type="gene ID" value="Metbo_1130"/>
    </dbReference>
    <dbReference type="GeneID" id="10277580"/>
    <dbReference type="KEGG" id="mel:Metbo_1130"/>
    <dbReference type="eggNOG" id="arCOG12048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718227_0_0_2"/>
    <dbReference type="OMA" id="CHETIFE"/>
    <dbReference type="OrthoDB" id="129743at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="152" mass="17417" checksum="DCEC818E6532FB9D" modified="2011-05-03" version="1">MKTQQNMRQMVFVPHRDGECLIGNSNTGRLHSLDCSAIDMMREDHKIPTDGAGFTPCGWCHACGTCHETIFEEVNEDPEGTELCMDKRIIQLFKQTGCASCSSKKGIIKMFHDPHGVRLIGREGHWWIYFECEECGYQTAWWKAMNKLKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F0T5Y3</accession>
    <name>F0T5Y3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644727.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09376">
        <property type="protein sequence ID" value="ADZ09376"/>
        <property type="gene ID" value="Metbo_1132"/>
    </dbReference>
    <dbReference type="GeneID" id="10277582"/>
    <dbReference type="KEGG" id="mel:Metbo_1132"/>
    <dbReference type="eggNOG" id="arCOG12084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933266_0_0_2"/>
    <dbReference type="OMA" id="NVRYEAK"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="134" mass="14726" checksum="CA330FCDB6097F3A" modified="2011-05-03" version="1" precursor="true">MESKDYAIILLVIILIAVVSFSLAYILASQPKTSSLNNSLNSSNASNVSTINSTTNKSQVQNQTKQQYITEQQAINIYKKETADHSNKYRYVATLFYIEGKPYYSITFIDKKTGDSSTSPGAVNAITGELEGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0T659</accession>
    <name>F0T659_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP004962</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645917.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2353"/>
    <dbReference type="EnsemblBacteria" id="ADZ10566">
        <property type="protein sequence ID" value="ADZ10566"/>
        <property type="gene ID" value="Metbo_2353"/>
    </dbReference>
    <dbReference type="GeneID" id="10278819"/>
    <dbReference type="KEGG" id="mel:Metbo_2353"/>
    <dbReference type="eggNOG" id="arCOG04847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682726_0_0_2"/>
    <dbReference type="OrthoDB" id="89246at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009183">
        <property type="entry name" value="UCP004962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09897">
        <property type="entry name" value="DUF2124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004962">
        <property type="entry name" value="UCP004962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="153" mass="16923" checksum="025C3CF3540F64E0" modified="2011-05-03" version="1">MKLEREFTGITENLLAFREEVKDADKVTFIGMPGVCSPFAELFAYVIRNKEAVFITGTNIETARKIEMTPQGMQFGETADPSSAVVAILGGLAMPKSNIDPEAVNELIDKVIQDDGKLLGLCYMDVFREAGWHELLDFDCIINATLNGFVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6A6</accession>
    <name>F0T6A6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644772.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1177"/>
    <dbReference type="EnsemblBacteria" id="ADZ09421">
        <property type="protein sequence ID" value="ADZ09421"/>
        <property type="gene ID" value="Metbo_1177"/>
    </dbReference>
    <dbReference type="GeneID" id="10277627"/>
    <dbReference type="KEGG" id="mel:Metbo_1177"/>
    <dbReference type="eggNOG" id="arCOG00017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133047_1_0_2"/>
    <dbReference type="OMA" id="CEKAVWY"/>
    <dbReference type="OrthoDB" id="125558at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="128" mass="14592" checksum="5F7B4BBBCA7AE002" modified="2011-05-03" version="1">MRPPCEIVVWYVIPSIRSELAKELLNLGMKQKEISELLDITQPAVSQYISDKRGHGIKFNDEVQELIKEFALDLKEGKLNQSGIISRICDLCRRIKTEDVVCQLHKEKDNIPVNCNACMGSQADHECH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6H1</accession>
    <name>F0T6H1_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rps12</name>
        <name evidence="5" type="ordered locus">Metbo_2392</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645956.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2392"/>
    <dbReference type="EnsemblBacteria" id="ADZ10605">
        <property type="protein sequence ID" value="ADZ10605"/>
        <property type="gene ID" value="Metbo_2392"/>
    </dbReference>
    <dbReference type="GeneID" id="10278859"/>
    <dbReference type="KEGG" id="mel:Metbo_2392"/>
    <dbReference type="eggNOG" id="arCOG04255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="OrthoDB" id="85293at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_S12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_S12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_S23_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15571" checksum="19D12A8CC7BD2B36" modified="2011-05-03" version="1">MPGLFAAKKLKKNRQNFRWKDVEYKRKALRLDVKADPLEGAPQARGIVIEKVGVEAKQPNSAIRKCVRVQLIKNGKQLTAFAPGDGAIGFIDEHDEVMIEGIGGPSGRSMGDIPGVRWKVTKVNNVSLQQMVIGKIEKPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6N4</accession>
    <name>F0T6N4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="1">5'-dA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="1">MTA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenosine deaminase</fullName>
            <ecNumber evidence="1">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dadD</name>
        <name evidence="3" type="ordered locus">Metbo_0014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.41" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EC" id="3.5.4.4" evidence="1"/>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643618.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0014"/>
    <dbReference type="EnsemblBacteria" id="ADZ08267">
        <property type="protein sequence ID" value="ADZ08267"/>
        <property type="gene ID" value="Metbo_0014"/>
    </dbReference>
    <dbReference type="GeneID" id="10276436"/>
    <dbReference type="KEGG" id="mel:Metbo_0014"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="OMA" id="ALIGKVC"/>
    <dbReference type="OrthoDB" id="40873at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090613">
        <property type="term" value="F:5'-deoxyadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="52"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="432" mass="47362" checksum="359ECEC6523B0141" modified="2011-05-03" version="1">METKNILIKNATLIADEIKKSSILIENDEIVEISNDLKFNDADEVIDASGKLVIPGLVNTHTHLSMTLMRGLADDMPLDTWLNDHIWPAEAELNGDYCYAGALLACAEMIKSGTTCFNDMYFFMDHVAKAVDESGMRGVLSHGMIDFGDETKRKNEFKETRRIIDKCHNSADGRIKVAYGPHSPYTCSQELLEEVKKEANKSGHRIHIHVSETQKEVSDSLESKGKRPFEYLSEIGFLGEEVTAAHAVWLSEDEISLIKNSQTKISHNPSSNMKLASGIAPVSKLLAEGASVSIGTDGTASNNNLDILEEMKITSLLQKVNTLDPSVLPAKEVYEMATINGAAAIGLEADIGTIEVGKKADMALLDIKTIRMTPFRNPMSHLVYSAQGGDVDTVICNGEVLMENKKLLTIDEAMVIEMAQNAAEEMLTKKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6S7</accession>
    <name>F0T6S7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643661.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0057"/>
    <dbReference type="EnsemblBacteria" id="ADZ08310">
        <property type="protein sequence ID" value="ADZ08310"/>
        <property type="gene ID" value="Metbo_0057"/>
    </dbReference>
    <dbReference type="GeneID" id="10276480"/>
    <dbReference type="KEGG" id="mel:Metbo_0057"/>
    <dbReference type="eggNOG" id="arCOG02448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046240_0_0_2"/>
    <dbReference type="OMA" id="MNHFKGH"/>
    <dbReference type="OrthoDB" id="20253at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007160">
        <property type="entry name" value="DUF362"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04015">
        <property type="entry name" value="DUF362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="187"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="368" mass="40758" checksum="6B22EE442B8267F5" modified="2011-05-03" version="1">MVSKVYFTDFRSKSNQDNKVNKVRNLFDAINFQAFIDENDLMAVKLHFGEEGNDSYINPVLVRQVVDKITAQGAKPFLTDTNTLYYGSRHNSQDHLKTAILHGFDYSVVGAPLIIADGLRGENWANVQIKQKHFENVKIAGDILNADGMVVLSHFKGHEMAGFGGAIKNLAMGCASAPGKIEQHECSKPIINEGCNSCGRCADSCPVSAFEISKAGAAINYDKCIACNNCLGACPDELIKLNWSTMEEFIERMTEYALGAVKNKTEKMGYMNFLMNITPECDCLPYSDSPIVPDIGIMVSKDPVALDAACYDMVNEQCGLENSMLKHNHQHGEDKFRGLWKNVDGRRQLQYGEEIGLGNIKYDLVDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0T6V3</accession>
    <name>F0T6V3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FeS cluster assembly scaffold protein NifU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644824.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1231"/>
    <dbReference type="EnsemblBacteria" id="ADZ09473">
        <property type="protein sequence ID" value="ADZ09473"/>
        <property type="gene ID" value="Metbo_1231"/>
    </dbReference>
    <dbReference type="GeneID" id="10277682"/>
    <dbReference type="KEGG" id="mel:Metbo_1231"/>
    <dbReference type="eggNOG" id="arCOG02077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_5_1_2"/>
    <dbReference type="OMA" id="YKNPRNY"/>
    <dbReference type="OrthoDB" id="89731at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017787">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03419">
        <property type="entry name" value="NifU_clost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="130" mass="14019" checksum="8D1BABA56845D0B5" modified="2011-05-03" version="1">MYSEKVMDHFQNPRNVGEIEGASGVGTEGNPTCGDLMTIYITVEDNIITDIKFKTFGCGAAIATSSMITEMAVGKTIEEALKITRNDVADELEGLPPVKMHCSNLAADALKAAIADYKMKQAEKEKAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T714</accession>
    <name>F0T714_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1616 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644885.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1292"/>
    <dbReference type="EnsemblBacteria" id="ADZ09534">
        <property type="protein sequence ID" value="ADZ09534"/>
        <property type="gene ID" value="Metbo_1292"/>
    </dbReference>
    <dbReference type="GeneID" id="10277743"/>
    <dbReference type="KEGG" id="mel:Metbo_1292"/>
    <dbReference type="eggNOG" id="arCOG02884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840573_0_0_2"/>
    <dbReference type="OMA" id="TYYGETW"/>
    <dbReference type="OrthoDB" id="64460at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011674">
        <property type="entry name" value="DUF1616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07760">
        <property type="entry name" value="DUF1616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1616" evidence="2">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="141" mass="15926" checksum="2C26E1CBA2055EAF" modified="2011-05-03" version="1" precursor="true">MKFDKVASLILLILLAASIVTVIYITVNPQSGEKFTEFYILGENGKAGNYPTNLTLGETGNLTMGIVNREQNTTSYYLVVQLNGTKLYNNTFTLTKNETKEIPLNFKATTTGQNQKMEFMLYKLPDNTDVYRSLTMYINVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>F0T725</accession>
    <name>F0T725_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin-disulfide reductase</fullName>
            <ecNumber evidence="4">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_2446</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2446"/>
    <dbReference type="EnsemblBacteria" id="ADZ10659">
        <property type="protein sequence ID" value="ADZ10659"/>
        <property type="gene ID" value="Metbo_2446"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2446"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="OrthoDB" id="41707at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="3">
        <location>
            <begin position="4"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ10659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="304" mass="33196" checksum="88375214AD088934" modified="2011-05-03" version="1">MEEYDIIIVGAGPAGLTAGIYAGREGLKSAVIEKNLRGGNANTAPVILNYPGYKSIPGMELLKKIAEQAEKYTEIKENEEIKKIEKIDGKFKIESSKNEYLTKSLIFCSGTTYRKIGVAGEEEYVGRGISYCSICDGMLFKGRDVVVVGGGNSAAEHALHLNDIGVNVKLIHRRGELRAQKYLQEKLKEEGIPILWNTTLKEIKGNMFIESVVLYNSKTKQEQELKVNGIFIAVGEVPNSQIANDLGVEIDDLNYIVADRDQKTNIPGVYTAGDITGGVNQLVVACGEGAVAAVNAYNYIKRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T751</accession>
    <name>F0T751_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MotA/TolQ/ExbB proton channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Metbo_2472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2472"/>
    <dbReference type="EnsemblBacteria" id="ADZ10685">
        <property type="protein sequence ID" value="ADZ10685"/>
        <property type="gene ID" value="Metbo_2472"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2472"/>
    <dbReference type="eggNOG" id="arCOG03209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088835_0_0_2"/>
    <dbReference type="OMA" id="LQFKQRW"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="98"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ10685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="214" mass="23736" checksum="D1188DCCC28CD6BA" modified="2011-05-03" version="1">MVIASIENTFNSSLHIITQSFLIPVMAVLVIFFIYSLINLGILIAQYYKRKQKKFDFKHFIDQFLMESRNGNTAELSNIIESAKISRSHKEVLTTLLTTDNVSNEFRESLALKMVEDETINSAKNLEKTDIVAKIAPAIGLMGTLIPLGPGLTALGAGDIQSLAQHLTIAFDAAVLGMASAAIAFIVSKVRRRWYEEEISNLETLVDTVLEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T756</accession>
    <name>F0T756_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="10">1.8.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_2477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the HdrA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646041.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2477"/>
    <dbReference type="EnsemblBacteria" id="ADZ10690">
        <property type="protein sequence ID" value="ADZ10690"/>
        <property type="gene ID" value="Metbo_2477"/>
    </dbReference>
    <dbReference type="GeneID" id="10278951"/>
    <dbReference type="KEGG" id="mel:Metbo_2477"/>
    <dbReference type="eggNOG" id="arCOG02236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="FICHCGD"/>
    <dbReference type="OrthoDB" id="1148at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="601"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="771" mass="83747" checksum="B3433C2ED09041FA" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T770</accession>
    <name>F0T770_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2491"/>
    <dbReference type="EnsemblBacteria" id="ADZ10704">
        <property type="protein sequence ID" value="ADZ10704"/>
        <property type="gene ID" value="Metbo_2491"/>
    </dbReference>
    <dbReference type="GeneID" id="10278965"/>
    <dbReference type="KEGG" id="mel:Metbo_2491"/>
    <dbReference type="eggNOG" id="arCOG01632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060275_0_0_2"/>
    <dbReference type="OMA" id="KWALIWW"/>
    <dbReference type="OrthoDB" id="37084at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methyltransf_25"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="68"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="32121" checksum="2E247CC61C504BDE" modified="2011-05-03" version="1">MDEHNGLIDPLNDINWSYYWQKELMELPPSNKSKDWDNIAKKFKEWMKKDDYPEKLLDKIKTHPNNSVLDIGCGEGTITLEIAKHVSEVTCIDLSTEMLEILDQKAQTEGIKNLTYLQGDMMDISLESLGEKDVVVASRCLNGVMEIETILRKLNNIGKSVYITLRHSGNFNPETNISKIINREYPKYPSYMYVCNILHKMGITANVEKLECDTLNIYEDMDEVMDRYRWKMGGLAHDEEMLLRDHFKKILVTNEEGYLENPEESSDWILIWWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>F0T776</accession>
    <name>F0T776_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved repeat domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013646061.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2497"/>
    <dbReference type="EnsemblBacteria" id="ADZ10710">
        <property type="protein sequence ID" value="ADZ10710"/>
        <property type="gene ID" value="Metbo_2497"/>
    </dbReference>
    <dbReference type="GeneID" id="10278971"/>
    <dbReference type="KEGG" id="mel:Metbo_2497"/>
    <dbReference type="eggNOG" id="arCOG02519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_457599_0_0_2"/>
    <dbReference type="OrthoDB" id="43071at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="2">
        <location>
            <begin position="104"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="376"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="596" mass="62391" checksum="722CD28AC029300B" modified="2011-05-03" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7C9</accession>
    <name>F0T7C9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AcnX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0105"/>
    <dbReference type="EnsemblBacteria" id="ADZ08358">
        <property type="protein sequence ID" value="ADZ08358"/>
        <property type="gene ID" value="Metbo_0105"/>
    </dbReference>
    <dbReference type="GeneID" id="10276530"/>
    <dbReference type="KEGG" id="mel:Metbo_0105"/>
    <dbReference type="eggNOG" id="arCOG04278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018825_1_0_2"/>
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    <dbReference type="OrthoDB" id="31127at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01355">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AcnX_catalytic_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcnX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="396" mass="42707" checksum="56BB3A7ED9BE5C6F" modified="2011-05-03" version="1">MHLTKEEERMYEGEEGSAVAKSMEILVALGDIYGAEKLVSISSAQISGVSYKTIGDAGLEYLQELSVDAHVRVPSTLNPAGVDLEIWKELGFSEEFTLKQQAIVEAYQKMGVSTTCTCTPYLVGNVPTLGSHIAWSESSAVCYANSVLGARTNREGGPGALSAAICGRTAEYGYHMDEARKPVLIVDVDTEIQGSDYGALGYLVGNKVGGGVPYFKFKNHPGTDQLKWLGAALASSGSVALYHVENVTPESSWAKQYTLNEEIETISVGREDINETRATLTTSDEKPDLICVGCPHASLEEIKQVADLLKGRKIKNMVWVCTSISIKAAADRMGYTKTIETAGGKLVCDTCMVVAPVEQLDYKVVGVDSAKAANYVPNMCGLDVVYNELENLIIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7H2</accession>
    <name>F0T7H2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe(3+)-transporting ATPase</fullName>
            <ecNumber evidence="5">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.30" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0149"/>
    <dbReference type="EnsemblBacteria" id="ADZ08401">
        <property type="protein sequence ID" value="ADZ08401"/>
        <property type="gene ID" value="Metbo_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0149"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="TQRFSYW"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="307" mass="34202" checksum="D16F955C51186025" modified="2011-05-03" version="1">MASEDVMIKIESVNKSFGNIKALDNLNLDIYRGELLGIIGPNGAGKTTTIRLTCCVLKPDSGDIVVDGSSIHDDPITIKSKIGYLPEEPNLYERFKARDLLRYFAELYGVPKNQIEPRIEELLELVGMTHRSEDRINTFSKGLRQRISVARALIHDPEIIIFDEPTMGLDPATAASIRNFIRQLKGKKTIILCTHYMEEADLLCDRVAILNKGRVGDVGTPDYLKSKIHGDVILTVKTDSKREPTTIKDQITQFESVGTVKFADGIFSITLKHRNEIAAIVDVFGSHVVSVNTQEPTLNDVFIHSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7J2</accession>
    <name>F0T7J2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644911.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1318"/>
    <dbReference type="EnsemblBacteria" id="ADZ09560">
        <property type="protein sequence ID" value="ADZ09560"/>
        <property type="gene ID" value="Metbo_1318"/>
    </dbReference>
    <dbReference type="GeneID" id="10277769"/>
    <dbReference type="KEGG" id="mel:Metbo_1318"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_4_0_2"/>
    <dbReference type="OMA" id="FASEMVI"/>
    <dbReference type="OrthoDB" id="96698at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="376" mass="41223" checksum="56ECE998FE5D5FAD" modified="2011-05-03" version="1">MKISIVIPALNEEGIVGKTVRSVPVEELERNGLETEIVVVDNASTDNTANEAREAGARVVLGPKRGYGNAYLTGFKEAKGDIIVMGDADGTYPFPMTYEFIQPILKGEADFVMGSRLKGDIKPGAMPALHKYVGNPFLTWILNFLFHAGISDAHCGMRAIRKETAEKLELKSGGMEFASEMVIEAARKHVKIAEIPIEYYPREGESKLSSFADGWRHLRFMMLYRPTPFLFAPGIVALAVGIFLTLTVAAAGTSRLHTLILGSLLLIIGYQMVLAWLHFGAFGSVYGYSKSSGIIEKIMSYHSLGRELFIGFLILAVGVVGGLYVLYSWSVTGYGSLYQLQYAMMALILSILGIQTIFSGMFLSLLLLANDYKTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7K5</accession>
    <name>F0T7K5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">THUMP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644924.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1332"/>
    <dbReference type="EnsemblBacteria" id="ADZ09573">
        <property type="protein sequence ID" value="ADZ09573"/>
        <property type="gene ID" value="Metbo_1332"/>
    </dbReference>
    <dbReference type="GeneID" id="10277783"/>
    <dbReference type="KEGG" id="mel:Metbo_1332"/>
    <dbReference type="eggNOG" id="arCOG00084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451423_0_0_2"/>
    <dbReference type="OMA" id="FIATCPP"/>
    <dbReference type="OrthoDB" id="82309at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THUMP" evidence="1">
        <location>
            <begin position="54"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="186" mass="21134" checksum="B258D64954234715" modified="2011-05-03" version="1">MKNTEKFDLVVKFNSSNLSNEGSIYDTYEQIDEIEGIDEIEGALTLRDPELYIKESESFDVVLVELGTDSLEAAKLLRNSNPRIVSTVIPIRRVVKTDLREIGRNLKELAIKEMEPGDDFTVETSVISHKTIESTDILEKVEDELKEINMNFDETDPKWKIYVEVIGESTGLNILKSEESILCMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7L8</accession>
    <name>F0T7L8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="9" type="ordered locus">Metbo_1346</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644937.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1346"/>
    <dbReference type="EnsemblBacteria" id="ADZ09586">
        <property type="protein sequence ID" value="ADZ09586"/>
        <property type="gene ID" value="Metbo_1346"/>
    </dbReference>
    <dbReference type="GeneID" id="10277797"/>
    <dbReference type="KEGG" id="mel:Metbo_1346"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_626450_0_0_2"/>
    <dbReference type="OMA" id="TPMSCEK"/>
    <dbReference type="OrthoDB" id="29893at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="461" mass="52249" checksum="73EE76BA4EDEB612" modified="2011-05-03" version="1">MLSVAFINPLSDEGKNVVRELGDFERVLADNDELISIVTESRSQEISDDSFIPRNYIDLAIKRIEWYVKKKNDRNFDNNIYEFLFNPEIYKFDIISYYLLCQAVGAKYGSNSRESRVLVESQGKIMEYRLGKLSKSEQTLAVNNILNSVVSLSHPRWTFFEELLGSKKLRLHEMILDNGEVILDKDDFLERFSSKIHGRKPDKMYEALIGTRVKELVMIKMVMQNTEDYISNVSAKSQREIEPNPILLQLADKISEVLAESARSYGFGGGSRGGSIRASALNPKAFPPCVKNVMEGIKSGGRNDAIIMFLTPFISYARLYPSVFSENISKRVSDIDPNLSVVEGEVMPLIFGAAERCTPPLFEDQPQDKVNLIAKLGFGMHETADLKNEGQTTWYTPMSCEKVKLHLPSLCKPDEHCKKIGNPLSYYNRMTWIIRKESQQSTGNEAGKTEAKTSKTQKDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0T7Z8</accession>
    <name>F0T7Z8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09624">
        <property type="protein sequence ID" value="ADZ09624"/>
        <property type="gene ID" value="Metbo_1384"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1384"/>
    <dbReference type="HOGENOM" id="CLU_2191046_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="108" mass="12536" checksum="073F3C75B84D03F3" modified="2011-05-03" version="1">MRLRTSRQKLYIILIKNIIYGGIYITKESSLFNPIFLALISLAIPGVGYLLLGYEKKGLYFLFSYAFLWLGYKLLENDFLIVSFLFLIIVIIISIYAAYDTYQLAENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F0T813</accession>
    <name>F0T813_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenylacetic acid degradation-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644990.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1399"/>
    <dbReference type="EnsemblBacteria" id="ADZ09639">
        <property type="protein sequence ID" value="ADZ09639"/>
        <property type="gene ID" value="Metbo_1399"/>
    </dbReference>
    <dbReference type="GeneID" id="10277850"/>
    <dbReference type="KEGG" id="mel:Metbo_1399"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_11_2_2"/>
    <dbReference type="OMA" id="FACNSHN"/>
    <dbReference type="OrthoDB" id="111444at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="2">
        <location>
            <begin position="47"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="136" mass="14719" checksum="566C7D62CD0DD9C4" modified="2011-05-03" version="1">MLEPEQIKEFFKNDKLAAYLGIELVDVSEGFAVSKMEIKEEHLNGINTAHGGAIFTLADFTFAVAANTHGTVTVAINGNMSFMKAAFKGGILTATATEVSINPKLGTYTVNVTDDNGDLIAIFQGMAYRKRDKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T839</accession>
    <name>F0T839_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP037465, zinc finger protein, AF1427</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645016.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1427"/>
    <dbReference type="EnsemblBacteria" id="ADZ09665">
        <property type="protein sequence ID" value="ADZ09665"/>
        <property type="gene ID" value="Metbo_1427"/>
    </dbReference>
    <dbReference type="GeneID" id="10277878"/>
    <dbReference type="KEGG" id="mel:Metbo_1427"/>
    <dbReference type="eggNOG" id="arCOG02570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184564_0_0_2"/>
    <dbReference type="OMA" id="ICIVCGM"/>
    <dbReference type="OrthoDB" id="112968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017211">
        <property type="entry name" value="UCP037465_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09947">
        <property type="entry name" value="DUF2180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037465">
        <property type="entry name" value="UCP037465_Znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="69" mass="7626" checksum="736DD3B943C4E157" modified="2011-05-03" version="1">MKCYIDALEGKDKDAVAICIVCGMALCMDHVKREDVTIWEGGYPFPSEKVSKSLPRILCPECYAALKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T871</accession>
    <name>F0T871_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643834.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0231"/>
    <dbReference type="EnsemblBacteria" id="ADZ08483">
        <property type="protein sequence ID" value="ADZ08483"/>
        <property type="gene ID" value="Metbo_0231"/>
    </dbReference>
    <dbReference type="GeneID" id="10276661"/>
    <dbReference type="KEGG" id="mel:Metbo_0231"/>
    <dbReference type="eggNOG" id="arCOG01684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090889_1_0_2"/>
    <dbReference type="OMA" id="MGCKTRR"/>
    <dbReference type="OrthoDB" id="87825at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="154" mass="17576" checksum="998BF4FEBD20747F" modified="2011-05-03" version="1">MELEAILDVMGCKTRRDIINLLTDEPRFVSEISKELEVGQKAIIEHLRAMEELGLLSSSFQKIERGRPRKYYDISQDIQIQIFIGPGAIKMNVVGQEFSDLHAIAGKIRMGETEVVEELENLIQRYDDGKKYAEQLLAEVKVHKGTQSQKSKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0T890</accession>
    <name>F0T890_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643853.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0250"/>
    <dbReference type="EnsemblBacteria" id="ADZ08502">
        <property type="protein sequence ID" value="ADZ08502"/>
        <property type="gene ID" value="Metbo_0250"/>
    </dbReference>
    <dbReference type="GeneID" id="10276682"/>
    <dbReference type="KEGG" id="mel:Metbo_0250"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_4_0_2"/>
    <dbReference type="OMA" id="IHCGDFV"/>
    <dbReference type="OrthoDB" id="78782at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ08502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="160" mass="17618" checksum="B1E02BB940350671" modified="2011-05-03" version="1">MIGIMSDSHDNIPAIRKAVETFNSMDVDLVIHAGDLISPFTANEFKNLEPEMVAVFGNNDGEREGLKKAYSEICLIEDFKEISVEGWKFSIIHGTNPQIVDALAKCGKYDVLIRGHTHELEIKGSETLIINPGEVCGYVSGKQTVVLVDPDDLNCEVINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T899</accession>
    <name>F0T899_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Metbo_0259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AL-21</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643862.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0259"/>
    <dbReference type="EnsemblBacteria" id="ADZ08511">
        <property type="protein sequence ID" value="ADZ08511"/>
        <property type="gene ID" value="Metbo_0259"/>
    </dbReference>
    <dbReference type="GeneID" id="10276691"/>
    <dbReference type="KEGG" id="mel:Metbo_0259"/>
    <dbReference type="eggNOG" id="arCOG03099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257387_0_0_2"/>
    <dbReference type="OMA" id="IIPFEPY"/>
    <dbReference type="OrthoDB" id="107043at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADZ08511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="103" mass="11567" checksum="575EFB7583330403" modified="2011-05-03" version="1">MFITRIYYGIAYFIVLLFEILKAEIDVIKRIINGDIEPVVVEIKTVLKRPVSQAILANSITLTPGTLSIDLDSENCILKVATISPRPVEEVIPFESYIKGMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8C6</accession>
    <name>F0T8C6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Metbo_0286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643889.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08538">
        <property type="protein sequence ID" value="ADZ08538"/>
        <property type="gene ID" value="Metbo_0286"/>
    </dbReference>
    <dbReference type="GeneID" id="10276718"/>
    <dbReference type="KEGG" id="mel:Metbo_0286"/>
    <dbReference type="eggNOG" id="arCOG04887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090238_3_2_2"/>
    <dbReference type="OMA" id="WSNHWIL"/>
    <dbReference type="OrthoDB" id="87417at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ08538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="201" mass="22771" checksum="7E495CCC55335CE7" modified="2011-05-03" version="1">MNSDNQLMDTKRLETLVDGIFAIAMTLLVLSLAVPQLTPPVTDSALRAALVGLIPNLISMVVSFLLLSIFWKIHHKFFKQIEYINGTLLWINVIWLLFIVLVPFSETLTGDYGGTMTAHFVFNLNMMGIALLLFLNWHYASSHNFINENVDQKEIKITWRVNVAFVLIVIGAILLSFVIPDYASIIYLLTFVVENIVRKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8E4</accession>
    <name>F0T8E4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09695">
        <property type="protein sequence ID" value="ADZ09695"/>
        <property type="gene ID" value="Metbo_1458"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1458"/>
    <dbReference type="HOGENOM" id="CLU_1559499_0_0_2"/>
    <dbReference type="OrthoDB" id="142938at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="171" mass="20089" checksum="75A42762CBFE3337" modified="2011-05-03" version="1">MNLRKEDVKKPWEKITIEEIQPYPPKYTKIIILSYLFILFNFFEFYNFFKIDTNILLPFTTCVYGVLIFVLLYLYSNGRLESFLFNLKDVAIVVTAYVIFVNFILIGTSHITMNTILRSLQGIFVLLLLFSGPIYLTISHHPRSEKHGNILIAIFIIYMSFSLVILAYNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8I7</accession>
    <name>F0T8I7_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645089.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09738">
        <property type="protein sequence ID" value="ADZ09738"/>
        <property type="gene ID" value="Metbo_1506"/>
    </dbReference>
    <dbReference type="GeneID" id="24964559"/>
    <dbReference type="KEGG" id="mel:Metbo_1506"/>
    <dbReference type="eggNOG" id="arCOG10420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157716_0_0_2"/>
    <dbReference type="OMA" id="NRWNIRI"/>
    <dbReference type="OrthoDB" id="113995at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="16880" checksum="6238006D68547E33" modified="2011-05-03" version="1">MKTQVEISKFIPEVFEFNFTSISFPYTILKYENGMFKCGYSDDEESIVPTEEDWAKFWKKLDRINAWDWAGNYVIPEYMYLDGNRWNIRIQFGDKKIECNGSNAYPGKNGEIVEHKMIFRRFFMAITKLLDIKTVDLKIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8L5</accession>
    <name>F0T8L5_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flpA</name>
        <name evidence="5" type="ordered locus">Metbo_0305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643908.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0305"/>
    <dbReference type="EnsemblBacteria" id="ADZ08557">
        <property type="protein sequence ID" value="ADZ08557"/>
        <property type="gene ID" value="Metbo_0305"/>
    </dbReference>
    <dbReference type="GeneID" id="10276737"/>
    <dbReference type="KEGG" id="mel:Metbo_0305"/>
    <dbReference type="eggNOG" id="arCOG00078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OMA" id="INMATHR"/>
    <dbReference type="OrthoDB" id="58340at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="79"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="122"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="25358" checksum="110677BAD268C031" modified="2011-05-03" version="1">MENLEDYTGLYVFEDHIATKNLNIGQKVYGEKLVEHEGDEFRIWDQRRSKLGAALMNGMKIYPFEIDSKILYLGASAGTTPSHISDISPDGSIWCVEFSPRMMRKMVEISKTRPNLIPLLEDATKPKNYAHLMEKVDVVYSDVAQPRQSELFMNNMNLYLKSEGIGIIMIKARSIDVTKSPKKIFKEEETKLKTGGFRILEKIKLEPYEKDHLAVVCEFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8P7</accession>
    <name>F0T8P7_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine_BP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643940.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0337"/>
    <dbReference type="EnsemblBacteria" id="ADZ08589">
        <property type="protein sequence ID" value="ADZ08589"/>
        <property type="gene ID" value="Metbo_0337"/>
    </dbReference>
    <dbReference type="GeneID" id="10276774"/>
    <dbReference type="KEGG" id="mel:Metbo_0337"/>
    <dbReference type="eggNOG" id="arCOG04373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137479_3_1_2"/>
    <dbReference type="OMA" id="EYETNPM"/>
    <dbReference type="OrthoDB" id="126214at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="SSF89957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00106">
        <property type="entry name" value="TIGR00106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine_BP" evidence="2">
        <location>
            <begin position="4"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="101" mass="10845" checksum="65DA78AD0732769B" modified="2011-05-03" version="1">MITAEITIIPIGTPNTSLSSYVAAAVMALEKNGIKYELTGMGTLLESEDISLVFDAIKEAHEAVFAVGAERVSTNVKIDDRRDSNRTISDKVSSVNKKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8R8</accession>
    <name>F0T8R8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="4">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mtrA</name>
        <name evidence="5" type="ordered locus">Metbo_0358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="4">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MtrA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="4"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013643961.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0358"/>
    <dbReference type="EnsemblBacteria" id="ADZ08610">
        <property type="protein sequence ID" value="ADZ08610"/>
        <property type="gene ID" value="Metbo_0358"/>
    </dbReference>
    <dbReference type="GeneID" id="10276795"/>
    <dbReference type="KEGG" id="mel:Metbo_0358"/>
    <dbReference type="eggNOG" id="arCOG03221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100863_0_0_2"/>
    <dbReference type="OMA" id="EVKGHIT"/>
    <dbReference type="OrthoDB" id="89197at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="239" mass="25507" checksum="214A0DDC4DF15ECF" modified="2011-05-03" version="1">MADKKSPAEGWPVINGDYVTGDPESPVAVATLASHNEQIPVDAGAAIAGPCKTENLGIEKMISNLISNPNLRFLILCGAEVQGHITGESIKALHENGVDPEKRSIVGAPGAIPYIENVPDAGLDRFRSQFEIVDLIDVEDAAAIQAKVKECIEKDPGAIEEEAMVIEIEEEGEEEEGEEVRPVAPETALIEARMRTIQSEVKAVGSIQRQFAGMYAGKVQGIVVGLAFILTLGTILLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8T1</accession>
    <name>F0T8T1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
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                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
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                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        </source>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V2</accession>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="52" mass="6192" checksum="D954B03EEBBC4425" modified="2011-05-03" version="1">MDVNNDLDINLIVNDEESPEVAIEIFQLYDHELYVEALREQVKFSPKEERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8V4</accession>
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                <person name="Lapidus A."/>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">AL-21</strain>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Metbo_1554"/>
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        <property type="entry name" value="NOV_C"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3883" evidence="1">
        <location>
            <begin position="210"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="327" mass="38196" checksum="A0E9549A3555E109" modified="2011-05-03" version="1">MESVNKRIIFLNVGWMKSYQGIINGDIIQGGGSYVHENKFGHEVYNFLPYDGFVYGFVQPPRSSKIHIERLGADPKDDSIDNVLAIWVATKPTSGNVIVGWYKDATLYKEYQYSDELNRNFKDEKIGYNVKAKEEDSKLLALDERVFHIKRGKGWMGQSNIWYASQESNEDFRNEVLNYIYENIIPKKNDKKRNHSISRQVDTYQRQKIEVTAINHTIKYYEKYGYTVESKEKDNVGWDLEAVMDNIKLKIEVKGLSQENVAIELTPNEYEKMKEFKEDYRISVVTNALKKPILRIFSYNDENGIWQDIEGNKLEIDEKVSAKCYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W3</accession>
    <name>F0T8W3_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SMC domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645142.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1564"/>
    <dbReference type="EnsemblBacteria" id="ADZ09791">
        <property type="protein sequence ID" value="ADZ09791"/>
        <property type="gene ID" value="Metbo_1564"/>
    </dbReference>
    <dbReference type="GeneID" id="10278020"/>
    <dbReference type="KEGG" id="mel:Metbo_1564"/>
    <dbReference type="eggNOG" id="arCOG00202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_353612_0_0_2"/>
    <dbReference type="OMA" id="EGHQDGM"/>
    <dbReference type="OrthoDB" id="64903at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="815" mass="92234" checksum="0457BD993021F778" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W4</accession>
    <name>F0T8W4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645143.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1565"/>
    <dbReference type="EnsemblBacteria" id="ADZ09792">
        <property type="protein sequence ID" value="ADZ09792"/>
        <property type="gene ID" value="Metbo_1565"/>
    </dbReference>
    <dbReference type="GeneID" id="24876083"/>
    <dbReference type="KEGG" id="mel:Metbo_1565"/>
    <dbReference type="eggNOG" id="arCOG07683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057493_0_0_2"/>
    <dbReference type="OMA" id="RMIFLAD"/>
    <dbReference type="OrthoDB" id="68548at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="365" mass="42199" checksum="2765B7DD3B0B4A80" modified="2011-05-03" version="1">MSSKIFMIKNKEKLVAMDEKKYVNELDFQDLIEEYPDLMPGDQINSENPRKWLLVKRELGIPFEEEGNKLLSLDHFFLDQDGIPTLVEVKRSCDTRLRREVVAQMLDYAANIVSYLPFEEIKNTLISKYDDITLIDDFLDEDMNENVYWNNLESNLKNGKIRLLFVADEIPKELQTIIEFLNNQMDKTEVLGVEIKQYLAEEEQTKTLVSRIIGQSAEIQSKGGKTQYLTKDTFLENVDTYGKNFFILLFKFASENDLIINWGTKGFSLNVKIDNKNVSLLQGYSNLRANGQIMYSTINSIIKKVENGDEVVDKFISKALTIDNFIKINGGFMFDLKRNLQSDEWNKFKDLVLNTIGDIKKNGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T8W8</accession>
    <name>F0T8W8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zinc_ribbon_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645147.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1569"/>
    <dbReference type="EnsemblBacteria" id="ADZ09796">
        <property type="protein sequence ID" value="ADZ09796"/>
        <property type="gene ID" value="Metbo_1569"/>
    </dbReference>
    <dbReference type="GeneID" id="10278025"/>
    <dbReference type="KEGG" id="mel:Metbo_1569"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134233_0_0_2"/>
    <dbReference type="OMA" id="CGTENDD"/>
    <dbReference type="OrthoDB" id="127916at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="2">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="131" mass="13425" checksum="61F64512A23334F7" modified="2011-05-03" version="1">MTYCKKCGSENTNGATFCKKCGESMDSVEIKKTSRTVELVLGILGGIFGLLGGVFALMFSAFAPSVASLGASAVIASIVGLIGSAYVLQNPKWGGIILIVSAVWLLISISLFGVLGAVLLGLAGLLAVLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T906</accession>
    <name>F0T906_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LapA_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_0376</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0376"/>
    <dbReference type="EnsemblBacteria" id="ADZ08628">
        <property type="protein sequence ID" value="ADZ08628"/>
        <property type="gene ID" value="Metbo_0376"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0376"/>
    <dbReference type="HOGENOM" id="CLU_3113065_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010445">
        <property type="entry name" value="LapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06305">
        <property type="entry name" value="LapA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="LapA_dom" evidence="6">
        <location>
            <begin position="7"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ08628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="50" mass="6002" checksum="E459A182F4EE429B" modified="2011-05-03" version="1">MINLKTILVGFGFFLAVLVYVIHKFRIKSANKKRTEKIKKRKKKKINQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0T940</accession>
    <name>F0T940_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644013.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0410"/>
    <dbReference type="EnsemblBacteria" id="ADZ08662">
        <property type="protein sequence ID" value="ADZ08662"/>
        <property type="gene ID" value="Metbo_0410"/>
    </dbReference>
    <dbReference type="GeneID" id="10276847"/>
    <dbReference type="KEGG" id="mel:Metbo_0410"/>
    <dbReference type="eggNOG" id="arCOG00984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_2"/>
    <dbReference type="OMA" id="IRFGPGC"/>
    <dbReference type="OrthoDB" id="22099at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="14"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="381" mass="41548" checksum="7E0BAA545B95C246" modified="2011-05-03" version="1">MEANFELRKFVAPEFVFGLDARLLVGRYLNNFAAKKILLVTDRGIISSGWVDSVVRVLESDCYSYEIYSKIKPNPSTDDVMKGADLYLDEGCDVIVAIGGGSPMDCAKGIGIVSSNNKNIKEFEGVDKVSLPSPPLICIPTTAGSSADVSQFAIISDKSQGSKMAIISKKLVPDVALIDPVTTTTMNKYMTIYAGFDILSHAIEANVSNASSPITDIHSNEAIKLYSSNILPVLDDPDNIKYRGNLMLASLHAGLAFSNASLGLVHAMAHSLGGLKDLPHGELNALLLEHVVEFNFEYEPERYLKIANNMCMGKATNKTEFLLMIQELKENLGINHTLKDRGVNLEDLKNLSYRAMNDPCIATNPIIPKQSDVEAIFKNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F0T975</accession>
    <name>F0T975_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644048.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0445"/>
    <dbReference type="EnsemblBacteria" id="ADZ08697">
        <property type="protein sequence ID" value="ADZ08697"/>
        <property type="gene ID" value="Metbo_0445"/>
    </dbReference>
    <dbReference type="GeneID" id="10276883"/>
    <dbReference type="KEGG" id="mel:Metbo_0445"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217600_0_0_2"/>
    <dbReference type="OMA" id="NFPISWL"/>
    <dbReference type="OrthoDB" id="67655at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="1">
        <location>
            <begin position="89"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="221" mass="25365" checksum="AF88A422F55F2D73" modified="2011-05-03" version="1">MVSSRSKPHPSISQVKIIADKNNFPISWLNKQGFCEYGIFLENVKGIEIKPTKAMVEGTQEHLILENNFKKDAEPATFDEMLETSKTGEILSRELPVISEKFGIRGFIDEVWMTPDEFIIIDDKPGRVAYSSSINQVYGYCLAFKDMVPEKRRIIASLRERGTDNIFWSSYFDKKAEMDVKNLINRVHGLLNGKIDFIPTKNPNKCRGCRFNNLCDKKSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0T995</accession>
    <name>F0T995_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645197.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1619"/>
    <dbReference type="EnsemblBacteria" id="ADZ09846">
        <property type="protein sequence ID" value="ADZ09846"/>
        <property type="gene ID" value="Metbo_1619"/>
    </dbReference>
    <dbReference type="GeneID" id="10278076"/>
    <dbReference type="KEGG" id="mel:Metbo_1619"/>
    <dbReference type="eggNOG" id="arCOG15128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2802296_0_0_2"/>
    <dbReference type="OMA" id="QNDDQET"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="75" mass="8389" checksum="AE4C021D9B9B9CDB" modified="2011-05-03" version="1">MPELGPMGRGGARRRTRRRMTSLNSMQNDDQETETSPQTESVSSQEGSSDKLVKLAKLLKDKGVISESEYNLIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9A1</accession>
    <name>F0T9A1_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1625"/>
    <dbReference type="EnsemblBacteria" id="ADZ09852">
        <property type="protein sequence ID" value="ADZ09852"/>
        <property type="gene ID" value="Metbo_1625"/>
    </dbReference>
    <dbReference type="GeneID" id="25394738"/>
    <dbReference type="KEGG" id="mel:Metbo_1625"/>
    <dbReference type="eggNOG" id="arCOG04921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OMA" id="ADHRNGY"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="302"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="377"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="488"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="419"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="707" mass="80904" checksum="829CC31F96BAE2DF" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9E0</accession>
    <name>F0T9E0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GyrI-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1665"/>
    <dbReference type="EnsemblBacteria" id="ADZ09891">
        <property type="protein sequence ID" value="ADZ09891"/>
        <property type="gene ID" value="Metbo_1665"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1665"/>
    <dbReference type="eggNOG" id="arCOG03201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083625_0_0_2"/>
    <dbReference type="OMA" id="GKHHEIY"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008319">
        <property type="entry name" value="UCP031644"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031644">
        <property type="entry name" value="UCP031644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GyrI-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="209" mass="24435" checksum="20645511C2A581C1" modified="2011-05-03" version="1">MTIAKIDLKKENRELYYPKKGQVNLIDVPEMNFLLLDGEGDPNTSQEYQNAMETIFPVSYKVKFMSKKELDRDYVVMPLEGLWWTDNMEDFSVEDKDGWKWTVMIRQPDFIDIEMVNSVVEEISKKKELPSIKQLRLEKFREGKSAQILHVGPFSEEGPTVEKLHEYIEANGYSFDGSLDGTKHHEIYLSDIRRSKPENLKTVIRQPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9M0</accession>
    <name>F0T9M0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="10">DGGGP synthase</shortName>
            <shortName evidence="10">DGGGPS</shortName>
            <ecNumber evidence="10">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Metbo_0516</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AL-21</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="10"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644119.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0516"/>
    <dbReference type="EnsemblBacteria" id="ADZ08768">
        <property type="protein sequence ID" value="ADZ08768"/>
        <property type="gene ID" value="Metbo_0516"/>
    </dbReference>
    <dbReference type="GeneID" id="10276961"/>
    <dbReference type="KEGG" id="mel:Metbo_0516"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OMA" id="DYFDYEI"/>
    <dbReference type="OrthoDB" id="104789at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="144"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADZ08768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="30326" checksum="7F8F650EB424DCD8" modified="2011-05-03" version="1">MNAYLEILRPFNALMGVVAVLLVALISGNFTLAVIVACVIVFIFTGAGNAINDYFDHKIDAINKPERPIPSGRIALKTALIYSISLFVISSIMAFIIGIVPGMIVVLSAVLMYLYAKRLKTSCLVGNLSIAFLTGLCFVFGGVVLNAVELSIILGVYAFLMTMAREIVKDMEDVEGDSIEGASTFPIKHGMRKSSILAAAFMLIASLTSPILYFIGIFNIIFLMVMLVAIYLFLSCAISTLRDQSVENTRKVSKQIKIGMGIVFMAFALGSPIVTNLLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9R9</accession>
    <name>F0T9R9_METLA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
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        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADZ09948">
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09948.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="142" mass="16957" checksum="6DE0D3F6F276A9F8" modified="2011-05-03" version="1">MVVIPNISERTFYIIIAAIAVVGLIFILIQIRRIKKAQKKVNYLGEELKLKKLELVKDDMRAVCQKKQKVDKPIYSPNFGAKHYKNLLRNVDESKRSLEFMKIEKKLSDLDKREEVFDRIKQKYEMDDNDFKRKISKYDREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9S7</accession>
    <name>F0T9S7_METLA</name>
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        <submittedName>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09956">
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        <property type="gene ID" value="Metbo_1732"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1732"/>
    <dbReference type="HOGENOM" id="CLU_3057127_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="6272" checksum="388C3334339AA7E7" modified="2011-05-03" version="1">MSPKNRWDEVESMLTLLLNEKEAIKLVDAIKESIKYDMERTLDRFASKNKLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0T9Z0</accession>
    <name>F0T9Z0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_0562</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0562"/>
    <dbReference type="EnsemblBacteria" id="ADZ08813">
        <property type="protein sequence ID" value="ADZ08813"/>
        <property type="gene ID" value="Metbo_0562"/>
    </dbReference>
    <dbReference type="GeneID" id="10277007"/>
    <dbReference type="KEGG" id="mel:Metbo_0562"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_0_2"/>
    <dbReference type="OMA" id="YLFYENE"/>
    <dbReference type="OrthoDB" id="93705at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02928">
        <property type="entry name" value="TIGR02928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc6_C" evidence="4">
        <location>
            <begin position="299"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ08813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="376" mass="42719" checksum="B0370EF7E9E94D71" modified="2011-05-03" version="1">MGIEDILLFDETIFKNIDAFNPDYVPDNYLHRENQMESLAICLRPALKGGRPINTVVLGSPATGKTTAINKIFNMVESNSDKVVCVYVNCQLHTTRFNIFSQIYNKIFGHMPPETGVPFSRIYGSIMKHLQKEKKSMVVALDDVSYLFHSKNANKIFYDILRAHEEFKGVKTGIFAILSDIEFRYMLDKNVTSVFIPQEVVFDPYSTPEMFDILKDRVRAGFYPDVISDELLNTIAEQASEVGDLRMGIDLLRVSGNFAEADASKTIEEKHLKQALSNTSSTNLRSTLKTLSNEDKLLLKAIAEYETDDLIAGDLYKLFNNAHPTSYASFDRKLNKLEFLRMIDTKFTGKGVKGNSRLIILRFDSEDILKCLERID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-12-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA13</accession>
    <name>F0TA13_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644187.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0585"/>
    <dbReference type="EnsemblBacteria" id="ADZ08836">
        <property type="protein sequence ID" value="ADZ08836"/>
        <property type="gene ID" value="Metbo_0585"/>
    </dbReference>
    <dbReference type="GeneID" id="10277030"/>
    <dbReference type="KEGG" id="mel:Metbo_0585"/>
    <dbReference type="eggNOG" id="arCOG01224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629745_0_0_2"/>
    <dbReference type="OMA" id="CITYAHG"/>
    <dbReference type="OrthoDB" id="126162at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="SSF158372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="77" mass="9108" checksum="8B6BE417BB51B26A" modified="2011-05-03" version="1">MDAVERIEKDIVLFAKNIKEIESIEIHEKDVQVIEMAKNYRKDTDYYLEKKDYLTSFGCITYAHGLLDAVRLMHELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA42</accession>
    <name>F0TA42_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645343.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1769"/>
    <dbReference type="EnsemblBacteria" id="ADZ09992">
        <property type="protein sequence ID" value="ADZ09992"/>
        <property type="gene ID" value="Metbo_1769"/>
    </dbReference>
    <dbReference type="GeneID" id="10278226"/>
    <dbReference type="KEGG" id="mel:Metbo_1769"/>
    <dbReference type="HOGENOM" id="CLU_1536673_0_0_2"/>
    <dbReference type="OrthoDB" id="141527at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="174" mass="19092" checksum="88D0790EE7CC0ECC" modified="2011-05-03" version="1" precursor="true">MKKELFAVFCVVTILAGTAPIYAANTTTTKTVVLTGNQTAKLTAVQTQLNDLITKIEGLKKTYNNTTKNKGLLSALNQYEKQATKLNTAITSYLKNPTSPANIKIKNFQIKTKQLQWKVNVTEKVLKKTNIKKPVKPIKPVVPVHPVGPEPIKKPVHPVVPVHPVSNCTTNATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA48</accession>
    <name>F0TA48_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAS/PAC sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_1775</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645349.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1775"/>
    <dbReference type="EnsemblBacteria" id="ADZ09998">
        <property type="protein sequence ID" value="ADZ09998"/>
        <property type="gene ID" value="Metbo_1775"/>
    </dbReference>
    <dbReference type="GeneID" id="25394743"/>
    <dbReference type="KEGG" id="mel:Metbo_1775"/>
    <dbReference type="eggNOG" id="arCOG03567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_71_2"/>
    <dbReference type="OMA" id="VFDINEL"/>
    <dbReference type="OrthoDB" id="16486at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="4">
        <location>
            <begin position="207"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="5">
        <location>
            <begin position="278"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="359"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ09998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="573" mass="66743" checksum="28D6E64DFF811CCB" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TA57</accession>
    <name>F0TA57_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacterium</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
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    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
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            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
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    </reference>
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    <dbReference type="EnsemblBacteria" id="ADZ10007">
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        <property type="gene ID" value="Metbo_1785"/>
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    <dbReference type="KEGG" id="mel:Metbo_1785"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <sequence length="254" mass="27890" checksum="E1E48F35163ED2E2" modified="2011-05-03" version="1">MVEVVAVLNQKGGSGKTTTAVNLAVGLALKGKKILLVDFDPQGNATTSLGLMKREMDNTMRDVLYGKCDIEEAVLETEHNGLSLIPANIKLSGIEAYLNAQTAPIAVLNNKLKNIKENYDYVFIDSPPTLNIIATNVLTASDSVLIPIQAEPFALEGMVDLLEVIDIVAEDLNSPTEIKGVLLTKFKPKTKLGREVKAEVQKYFEEELYSTEIPENIRVAEAPGYNKPVISYDPDCTGTKAYLKLTEEFLKRDE</sequence>
</entry>
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                <person name="Copeland A."/>
                <person name="Lapidus A."/>
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                <person name="Pagani I."/>
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                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    </evidence>
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    </evidence>
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</entry>
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    <accession>F0TAD4</accession>
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    <reference evidence="2" key="1">
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            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
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                <person name="Brauer S.L."/>
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            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="25394713"/>
    <dbReference type="KEGG" id="mel:Metbo_0635"/>
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    <dbReference type="OMA" id="EPYTRKY"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="229" mass="26863" checksum="C79D3BCC39227DD5" modified="2011-05-03" version="1">MNQHDHGNELRFLGKIGVDTRFLSLYRFNIFINNQRFSKFTRKREEQFLKNYPDFQIHRSGLFQRLCTRASRVLASSIKPREKILVPDDNSCTDFALKILLEPYKRKYGIQILSGNMVDFDPAKYDSIAMATTLDDEAVHVLNRLFNGEKMDVVHEINTHERDHKLIYPLQNIPSSWIVEWIENLGHECEFSIQTNYELETLIFLGNFVSDVRENILKSALFISENSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAF9</accession>
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    <reference evidence="4" key="1">
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                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
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                <person name="Ivanova N."/>
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                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Brauer S.L."/>
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            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_0660"/>
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        <property type="protein sequence ID" value="ADZ08911"/>
        <property type="gene ID" value="Metbo_0660"/>
    </dbReference>
    <dbReference type="GeneID" id="10277105"/>
    <dbReference type="KEGG" id="mel:Metbo_0660"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
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        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
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        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="252"/>
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    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="98"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="253" mass="28842" checksum="C1D74EDC25B5727E" modified="2011-05-03" version="1">MKTIPSEDLKLFHFDQRNKLIFILCTAILWILALVIYLTPNFNHWFLANFNILRTNYLFANLCYYFTTLTLSFIATPISLLYLASFKFNKLKPYRVVLLLSVMTLAIGIPIVDLIKYYGVVPRPWVLYPDINSIYYPGGHSFPSGHAFQAFAGTLPLIICFLTNDETFKRNLKKTVLAIILLVFAISLSLSRLIAGVHFPTDVLFGIGLAIIMMVIVIGALQYLLKTGKLNLQNEKWYALIFLALITIDICFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAL9</accession>
    <name>F0TAL9_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1870"/>
    <dbReference type="EnsemblBacteria" id="ADZ10091">
        <property type="protein sequence ID" value="ADZ10091"/>
        <property type="gene ID" value="Metbo_1870"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1870"/>
    <dbReference type="HOGENOM" id="CLU_2177892_0_0_2"/>
    <dbReference type="OrthoDB" id="121922at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="109" mass="12396" checksum="E390251516F58C4F" modified="2011-05-03" version="1">MNMVNEEKTVSVEEELDLGALSKEVDKFLEDLEKHIRISIIGSKAIDKLEDFTLDVGLKKTYLCAKNTEESYPILAKVIELFIKVYGATITMYPKGEKVCLELITPKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAM0</accession>
    <name>F0TAM0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645443.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1871"/>
    <dbReference type="EnsemblBacteria" id="ADZ10092">
        <property type="protein sequence ID" value="ADZ10092"/>
        <property type="gene ID" value="Metbo_1871"/>
    </dbReference>
    <dbReference type="GeneID" id="10278329"/>
    <dbReference type="KEGG" id="mel:Metbo_1871"/>
    <dbReference type="eggNOG" id="arCOG03886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204994_0_0_2"/>
    <dbReference type="OrthoDB" id="127817at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="60" mass="7032" checksum="420753E87DFBBFC3" modified="2011-05-03" version="1">MGCVTICISDELEIAFRRLVRLKYGEKQGKISRGATEAIFDWCQRERIKLLDEDQLLEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAN0</accession>
    <name>F0TAN0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645453.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1881"/>
    <dbReference type="EnsemblBacteria" id="ADZ10102">
        <property type="protein sequence ID" value="ADZ10102"/>
        <property type="gene ID" value="Metbo_1881"/>
    </dbReference>
    <dbReference type="GeneID" id="10278339"/>
    <dbReference type="KEGG" id="mel:Metbo_1881"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_0_2"/>
    <dbReference type="OMA" id="GNTYRES"/>
    <dbReference type="OrthoDB" id="66517at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="280" mass="31638" checksum="32D9A1D77609F0DB" modified="2011-05-03" version="1">MILKKKSFEIALCQMNVVESKDENLERAVSMIREANVNGATLVVLPEMFNCPYDNDKFVEYAENRKTSKSLKAISRAADENNVYVVAGSIPEESCGNIYNSSFVFDDRGEVLDVHRKIHLFDVEVSDGISFKESNTITPGDKVTVVETPFMKFGVAICFDLRFPELFRLMAMEGAKLVVVPGAFNMTTGPAHWETTIRTRAIDNQIYVVAVSPAKNDELSYVAYGHSMVVDPWGDVVETAKEEETIVYATINEDMVNKIREELPLLKNRREDIYELIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAR4</accession>
    <name>F0TAR4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644291.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0689"/>
    <dbReference type="EnsemblBacteria" id="ADZ08940">
        <property type="protein sequence ID" value="ADZ08940"/>
        <property type="gene ID" value="Metbo_0689"/>
    </dbReference>
    <dbReference type="GeneID" id="10277134"/>
    <dbReference type="KEGG" id="mel:Metbo_0689"/>
    <dbReference type="eggNOG" id="arCOG04904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121275_0_0_2"/>
    <dbReference type="OMA" id="FIFVASE"/>
    <dbReference type="OrthoDB" id="93447at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016762">
        <property type="entry name" value="UCP019464_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09886">
        <property type="entry name" value="DUF2113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019464">
        <property type="entry name" value="UCP019464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03291">
        <property type="entry name" value="methan_mark_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="185" mass="21637" checksum="E29A543C14FD4ADB" modified="2011-05-03" version="1">MKVECYDQNGAEVYEMIVKHILQEVQVSRSIGDMRVYVDPREPLFIIVVKLENASPPILIEDMAELEYNKSGNEMFIKIIDENYLPDLLKKLWETEGRNKVHQPNRFEVIVDDPKTEIPGMIVLDPRDDLKEKIYDAIFRIIPEGFRVIDHRSEGNIIALTCTDEMMKDEWIKKTEEIVAEVKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAS5</accession>
    <name>F0TAS5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CoB/CoM heterodisulfide reductase, subunit C</fullName>
            <ecNumber evidence="6">1.8.98.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Metbo_0700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AL-21</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">AL-21</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.1" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0700"/>
    <dbReference type="EnsemblBacteria" id="ADZ08951">
        <property type="protein sequence ID" value="ADZ08951"/>
        <property type="gene ID" value="Metbo_0700"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0700"/>
    <dbReference type="eggNOG" id="arCOG00372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068200_0_0_2"/>
    <dbReference type="OMA" id="QERCPRS"/>
    <dbReference type="OrthoDB" id="102216at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADZ08951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="331" mass="36513" checksum="145F8DD280445CA9" modified="2011-05-03" version="1">MKTLKRLKNMLSGEKEDKTETPQGEPVPESSKATPEETPVSTKTVKTEVKSTAPEQKSKATEVKSKAEDIKEDVKSKAEEIKSKTEDVSSDVKSKVEDISTDVKSKVEDVKSKAKDISEDAKSKASKTDEKIKKSEESKTDRSGKMTLLEGREKEVITRENIDPDFKNEVMDAGAESVALCFQCGTCTGACPSGRRTPYKIRNVVRKSVMGLKEEVIADDTIWMCTTCYECQERCPRGIKIVDVVKTVRNFAAQAGYMAPAHKMTGSFVIKTGHGVPINDATMALRKSVGLGELPPTTHQFPEALEEVQKIVKATGFDNLIGWNWDKAELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT2</accession>
    <name>F0TAT2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644309.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0707"/>
    <dbReference type="EnsemblBacteria" id="ADZ08958">
        <property type="protein sequence ID" value="ADZ08958"/>
        <property type="gene ID" value="Metbo_0707"/>
    </dbReference>
    <dbReference type="GeneID" id="10277152"/>
    <dbReference type="KEGG" id="mel:Metbo_0707"/>
    <dbReference type="eggNOG" id="arCOG10244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860730_0_0_2"/>
    <dbReference type="OMA" id="GCAPVRI"/>
    <dbReference type="OrthoDB" id="114952at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ08958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="137" mass="15780" checksum="668549F42CDA7E9E" modified="2011-05-03" version="1">MKKIEFESPLDSRKKLMTVLFWINRKAARTEGCAPFLIKKIETKDKTYIPEGTKLLKLTDPMIDDIIDSIETKNPVKFEIQMGSEYIKAELHEDAFCVKTEHSGEIEEEIVEKLGTELNKKFPSICESFKPRVTPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAT5</accession>
    <name>F0TAT5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644312.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0710"/>
    <dbReference type="EnsemblBacteria" id="ADZ08961">
        <property type="protein sequence ID" value="ADZ08961"/>
        <property type="gene ID" value="Metbo_0710"/>
    </dbReference>
    <dbReference type="GeneID" id="10277157"/>
    <dbReference type="KEGG" id="mel:Metbo_0710"/>
    <dbReference type="eggNOG" id="arCOG00464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_3_2"/>
    <dbReference type="OMA" id="EPYGKWK"/>
    <dbReference type="OrthoDB" id="93911at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037046">
        <property type="entry name" value="AlkA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="138"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="295" mass="34304" checksum="F34740D62A44674C" modified="2011-05-03" version="1">MKLQTNEGKLYPEDPFDFKQSLNFIDKFFPSSHDYVILENQLKTAVQLKNTVGFKIESTGTVEDPVLHYQLFSEQLDKNTVDKVIDRMTFFLSLDDDLKPFYKLARSDDVFKEILDDLHGLHQVKFLTPFEAVGWAILSQRISMNVSRKMKEKLTAYVGNNIEFEGHTFQAFPSPDQVKNMDLDELISIIKNKRKSEYLLNAAEAFSSVDEKFLRTGPLEDVKNWLMDIKGVGEWSAHLELIRGLGRMEDYTQDRMLRGCVEKHYGSTASNELDEIIKKYKEFPGYWEYYLRSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAU6</accession>
    <name>F0TAU6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0721"/>
    <dbReference type="EnsemblBacteria" id="ADZ08972">
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        <property type="gene ID" value="Metbo_0721"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0721"/>
    <dbReference type="eggNOG" id="arCOG01403">
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    <dbReference type="OMA" id="ERFRYLW"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="20"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ08972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="394" mass="44725" checksum="76A7EDBD19B06D84" modified="2011-05-03" version="1">MKIGVITSAYPEYEDDPHGIFVHRLMKEVTKKGHEVHVLAPYTGKMTNFQLDGVYVQKFNYFYPKRFQKLAGRSGMIDNVKEGVFVKFQFCSFIIFNLINSYKKLKDMDIVHVQWPIPNGLGALFLKKISKIPYINTIHGEEVYLSKKYHTVFLIKLLVNNSSKTITNSSATLKTCLNEGLDKKKLDIIPFGVDTSFYKPLNIVKDKKIFQILSVGYLIERKGFMYLISSISEVSKKHENVRLKIVGSGPQEKQLKDLITKLQLEKYIEILGNIPNDELLKMYNSSDLFVLPSIIDSQGNTEGLGVVLIEAMACGLPVIGSNIGGIPDIISDGETGLLFPQKDVVELSKSIIKLIENRILMEKIADKGYQMVKTNFSWEKIAAQYIDCYEKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAV0</accession>
    <name>F0TAV0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ08976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644327.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ08976">
        <property type="protein sequence ID" value="ADZ08976"/>
        <property type="gene ID" value="Metbo_0725"/>
    </dbReference>
    <dbReference type="GeneID" id="10277172"/>
    <dbReference type="KEGG" id="mel:Metbo_0725"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006888_0_0_2"/>
    <dbReference type="OMA" id="AVAYRIY"/>
    <dbReference type="OrthoDB" id="134233at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
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    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="35"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ08976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="275" mass="32757" checksum="64CB1EFCAD106087" modified="2011-05-03" version="1">MEKKYDLTVAYRIYPKISCTPAIYGDDKYKLSEFCLKSFKESLGNLKVKMIVLLDGCPPKYRELFLKYFDESDIDFFELDKIGNLPTFSLQINLLLKQNYSEIIYFAEDDYYYHPNQFFEMIDFFNKNDDVDFITPYDHSDYYNRNIHNIKRYERMYNGRQWKTASSTCLTFLTSKKILDETQNIFLTYQKGNTDLGLWLSLTKSSLDPKIIIKSLNIYTIKSLYKTFVLTQDQIHNGKTWKLWFPIPTIATHLEKEFISPTINWENIVSKELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-02-26" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>F0TAX6</accession>
    <name>F0TAX6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_0751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09002">
        <property type="protein sequence ID" value="ADZ09002"/>
        <property type="gene ID" value="Metbo_0751"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0751"/>
    <dbReference type="HOGENOM" id="CLU_1514626_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ09002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="177" mass="20888" checksum="AF85DCB61974DE4A" modified="2011-05-03" version="1">MDYNLKLKKCSRCKEYLPLNKFSIDKKTKSGHQSYCKKCRKEMYHENRHINKIKEKEYRMKNPINIWAMHTRNNHQRRGIKINISVKKLTELGEMAVKNGCYYCGTKFDIRNSQGKAGEFTSNAMTVDLIDSNRKVMDEQNIRILCHSCNSGKGTLNEEEFLLKCKKIVERWSNDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB08</accession>
    <name>F0TB08_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
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                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1934"/>
    <dbReference type="EnsemblBacteria" id="ADZ10154">
        <property type="protein sequence ID" value="ADZ10154"/>
        <property type="gene ID" value="Metbo_1934"/>
    </dbReference>
    <dbReference type="GeneID" id="10278392"/>
    <dbReference type="KEGG" id="mel:Metbo_1934"/>
    <dbReference type="HOGENOM" id="CLU_2534710_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="83" mass="9591" checksum="12888D0A23D8DF18" modified="2011-05-03" version="1">MKSKMKVEKKVGKLFLIDERKFYPIVEISTIESDNYFAETLTPVAFLVSEPTKKYILPLSEDEIDKEEIIRLVFPDGLNHTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB12</accession>
    <name>F0TB12_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_1938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645509.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1938"/>
    <dbReference type="EnsemblBacteria" id="ADZ10158">
        <property type="protein sequence ID" value="ADZ10158"/>
        <property type="gene ID" value="Metbo_1938"/>
    </dbReference>
    <dbReference type="GeneID" id="10278398"/>
    <dbReference type="KEGG" id="mel:Metbo_1938"/>
    <dbReference type="eggNOG" id="arCOG02708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164556_0_0_2"/>
    <dbReference type="OMA" id="YQVTLHD"/>
    <dbReference type="OrthoDB" id="124644at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="119" mass="13306" checksum="D538C4AAC4747B77" modified="2011-05-03" version="1">MKTNVDFISVQVKDLESASKFYTEILGFEKTEDKNPDATVFKDDGGAIFAIRKPMFKITESTPLGLGVSIWFGVDDIDKTFTLVKNSDAQIISPPVDGPFGKMFIIKDMDGYMLTFHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB34</accession>
    <name>F0TB34_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine--fructose-6-phosphate transaminase (Isomerizing)</fullName>
            <ecNumber evidence="3">2.6.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_1960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645531.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1960"/>
    <dbReference type="EnsemblBacteria" id="ADZ10180">
        <property type="protein sequence ID" value="ADZ10180"/>
        <property type="gene ID" value="Metbo_1960"/>
    </dbReference>
    <dbReference type="GeneID" id="10278420"/>
    <dbReference type="KEGG" id="mel:Metbo_1960"/>
    <dbReference type="eggNOG" id="arCOG00058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_2_1_2"/>
    <dbReference type="OMA" id="QSFYVMA"/>
    <dbReference type="OrthoDB" id="42859at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="335" mass="37625" checksum="5C6A36C5C47C573F" modified="2011-05-03" version="1">MKYDMYNEVQEQPKSLAETLKEEKDHMVEIAQKLKDTDKIYLVGCGSSLSTCYSARDALGFLSDRTIEVHTGYEFVHHKNLQKTNSALITTSQSGETADTLAALRRAKDEGIYTISITNEPESSMMKEADDAVLTRCGRETAILGTKTYMTQLLCLYQILFSMDGSEESGKILEDLDKIPSIAQKLVETTEEPNRELAKQYMDDDIFYCMGSGPNFGLAYKIAMTMLMEGALKHACPLYSGEFRHGLIERAEKDVPIIFLDAGFPGDELTNKSIDFSNKIGAKTITFNMGDFSDIHPLLSPFILVIPVEWFIYYLSHYSGEDPGSTRHIGKVRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>F0TB45</accession>
    <name>F0TB45_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Metbo_1971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645542.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1971"/>
    <dbReference type="EnsemblBacteria" id="ADZ10191">
        <property type="protein sequence ID" value="ADZ10191"/>
        <property type="gene ID" value="Metbo_1971"/>
    </dbReference>
    <dbReference type="GeneID" id="10278431"/>
    <dbReference type="KEGG" id="mel:Metbo_1971"/>
    <dbReference type="eggNOG" id="arCOG00203">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="RCIPFLE"/>
    <dbReference type="OrthoDB" id="92866at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ10191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="279" mass="30999" checksum="934F55B88D50ECCD" modified="2011-05-03" version="1">MKIIETNDLTYEYPDGTKALEKVNFNAEEGKIVALLGPNGAGKSTLFLHFNGILRPTHGSVHVDGETVSYDKKELLKIRQKVGIVFQNPDDQLFAPTVLEDVAFGPMNMGLSNEEVDERVKDALLRVGMEGYEKKAPHHLSGGQKKRVAIAGILAMKPKIMVLDEPTSGLDPRGASQILKLLYQLNSEGMTIVISTHDVDLVPIYASKVYIISQGKIIKEGNPQDVFEDVETIRGANLRLPRIAHLMEILKKEDKLPFDKPYPLTIGGARKRILKEFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBB6</accession>
    <name>F0TBB6_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps2</name>
        <name evidence="7" type="ordered locus">Metbo_0816</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AL-21</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644418.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0816"/>
    <dbReference type="EnsemblBacteria" id="ADZ09067">
        <property type="protein sequence ID" value="ADZ09067"/>
        <property type="gene ID" value="Metbo_0816"/>
    </dbReference>
    <dbReference type="GeneID" id="10277265"/>
    <dbReference type="KEGG" id="mel:Metbo_0816"/>
    <dbReference type="eggNOG" id="arCOG04245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="OMA" id="QQKTKDM"/>
    <dbReference type="OrthoDB" id="63341at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_S2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_S2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_S2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="PTHR11489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADZ09067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="198" mass="22127" checksum="9D94600FEE470AE7" modified="2011-05-03" version="1">MSELLIPLDKYLAAGLHIGTQQKTKDMERYIYRVRADGLYVLDVRKTNDRIVAAGKFLSKYDPEDILVVSTRQYGQAPVKKFGQITGAKTIPGRFIPGTLTNPNYAKFIEPKVLVVTDPRSDSQAIIEARQIGIPVVALCDTENLLGNVDIVVPVNNKGRKAIALVYWLLARQNLRGRGILKEDEDLETPATDFELKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBC0</accession>
    <name>F0TBC0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="7">IPP isomerase</shortName>
            <ecNumber evidence="7">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="7">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fni</name>
        <name evidence="9" type="ordered locus">Metbo_0820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AL-21</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644422.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0820"/>
    <dbReference type="EnsemblBacteria" id="ADZ09071">
        <property type="protein sequence ID" value="ADZ09071"/>
        <property type="gene ID" value="Metbo_0820"/>
    </dbReference>
    <dbReference type="GeneID" id="10277269"/>
    <dbReference type="KEGG" id="mel:Metbo_0820"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="OMA" id="WDWGIPT"/>
    <dbReference type="OrthoDB" id="42330at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="PTHR43665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_dh" evidence="8">
        <location>
            <begin position="161"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="5"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="259"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="280"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADZ09071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="351" mass="37739" checksum="7FDC4D1A648F524A" modified="2011-05-03" version="1">MISDRKLEHLLLCKNCDVEYRKKTGLGDVELIHKALPEVNMKEIDLSIDLLGKKLDSPFIISAITGGHPSATVINRTLARTAKILNIGMGVGSQRAAIKHPELTSTYTVVREEAPDAFLIGNIGCQQIELAQKSIEMIDADALAVHLNPLQEAIQPEGDVDARGHIESITEMTSTLETPIIAKETGAGIKAEDAITLEKAGVSAIDVAGSGGTSWAAVETYRAQDRTMGDAFWDWGIPTAASTVEVCQSVKIPVISSGGIRSGLDAAKAIALGADAVGIALPLLKDAYSGHEEVVNRINKFNEELRVAMFLVGASNIAELKKSDLIIKGETREWLNERGFQTSIYARRSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-08-12" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBD5</accession>
    <name>F0TBD5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_1984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10204">
        <property type="protein sequence ID" value="ADZ10204"/>
        <property type="gene ID" value="Metbo_1984"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_1984"/>
    <dbReference type="eggNOG" id="arCOG14558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3394520_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="31" mass="3532" checksum="25F04B25BD9AEAD5" modified="2011-05-03" version="1">MLPKFDLQHFCCGPKGCELEIVYGEMIDAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBF4</accession>
    <name>F0TBF4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645574.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10223">
        <property type="protein sequence ID" value="ADZ10223"/>
        <property type="gene ID" value="Metbo_2004"/>
    </dbReference>
    <dbReference type="GeneID" id="10278464"/>
    <dbReference type="KEGG" id="mel:Metbo_2004"/>
    <dbReference type="eggNOG" id="arCOG10346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773204_0_0_2"/>
    <dbReference type="OMA" id="NGAWTGN"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="146" mass="14744" checksum="5ED4EDC01ED96C67" modified="2011-05-03" version="1" precursor="true">MKKINFGILGILGIVLLVVFASGCTSNNSNSSSNGQSNQSNGQAATYSGAPAVKVTGNGAWTGNIADNSGSRSVDGSGSQTFQLTDDPGVVAVTFQKDNSNEVSQNGTITPNTGTLTVEILDKTGKVVATQSTTADAGVVSTSYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBH2</accession>
    <name>F0TBH2_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645592.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ10241">
        <property type="protein sequence ID" value="ADZ10241"/>
        <property type="gene ID" value="Metbo_2022"/>
    </dbReference>
    <dbReference type="GeneID" id="10278483"/>
    <dbReference type="KEGG" id="mel:Metbo_2022"/>
    <dbReference type="eggNOG" id="arCOG03597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157711_1_0_2"/>
    <dbReference type="OMA" id="IDPHEEC"/>
    <dbReference type="OrthoDB" id="118506at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="105" mass="12258" checksum="D2B09B7AE79225DF" modified="2011-05-03" version="1">MQQIEFSMENIKRCLCSVCDVQKRSECVKDRQKLMLLIKNQDLDSPMMMGPDKVPGLYCSTGKAVCQDLDTHEICKCDECPVWMEFSLDKFENNRYFCENGKFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBJ4</accession>
    <name>F0TBJ4_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ordered locus">Metbo_2044</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AL-21</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645614.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2044"/>
    <dbReference type="EnsemblBacteria" id="ADZ10263">
        <property type="protein sequence ID" value="ADZ10263"/>
        <property type="gene ID" value="Metbo_2044"/>
    </dbReference>
    <dbReference type="GeneID" id="10278506"/>
    <dbReference type="KEGG" id="mel:Metbo_2044"/>
    <dbReference type="eggNOG" id="arCOG01433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="OrthoDB" id="24741at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="51"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADZ10263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="393" mass="42846" checksum="2CB3B18C8CF7E4DD" modified="2011-05-03" version="1">MLEDGKFGKYGGIFVPELIIPALEELEEAFYKYKDDPEFNEELKFYLKEFAGRPTNLYFAENLSKKFGCRVYLKREDMLHTGAHKINNTLGQGLLAKYMGKTRLIAETGAGQHGIATAVVGALFKLPADVYMGSEDVERQKMNVFRMELSGGKVIPVETGSKTLKDAINEAFRDWITNVENTHYLIGSTMGPHPYPTMVKHFQSVIGEEARRQIMEKEGKLPDAVIACVGGGSNAMGIFAGFAADDEVELIGVEGGGDGTETPRTGATLCKGTEGILHGSLSFVLQDQDGQINEAHSVSAGLDYPGVGPEHAHMKVTGRANYVAVTNKEALRGFELLSKYEGIIPALESSHAVAYAEKYASMEENKGKTIIINLSGRGDKDMFIVAKEMGVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBM5</accession>
    <name>F0TBM5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_0852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644453.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADZ09102">
        <property type="protein sequence ID" value="ADZ09102"/>
        <property type="gene ID" value="Metbo_0852"/>
    </dbReference>
    <dbReference type="GeneID" id="10277301"/>
    <dbReference type="KEGG" id="mel:Metbo_0852"/>
    <dbReference type="eggNOG" id="arCOG13997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998778_0_0_2"/>
    <dbReference type="OMA" id="ANTYHKS"/>
    <dbReference type="OrthoDB" id="127666at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ09102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="138" mass="15057" checksum="233E7F98F23FAD5F" modified="2011-05-03" version="1">MIKKGIFIFVIIILIIIISGCTTKTYNGTFGEKTVSIDSIYLSNNTTANTYHKSSTGAQYYFVSGYLINNNSNDALKLKIASTAYDVNGNIIVTNNSANIYPSTIPAKGASEFYVEFPDNNNNIVKYEVKILSASGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBP6</accession>
    <name>F0TBP6_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Parallel beta-helix repeat</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Metbo_0874</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AL-21</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0874"/>
    <dbReference type="EnsemblBacteria" id="ADZ09123">
        <property type="protein sequence ID" value="ADZ09123"/>
        <property type="gene ID" value="Metbo_0874"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_0874"/>
    <dbReference type="eggNOG" id="arCOG02552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_309879_0_0_2"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001434">
        <property type="entry name" value="DUF11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01345">
        <property type="entry name" value="DUF11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01451">
        <property type="entry name" value="B_ant_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_helix" evidence="3">
        <location>
            <begin position="208"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="NosD" evidence="2">
        <location>
            <begin position="392"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="743"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="865"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="DUF11" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADZ09123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1104" mass="115407" checksum="20A25F2C60BC35BE" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBR4</accession>
    <name>F0TBR4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_0892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644492.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0892"/>
    <dbReference type="EnsemblBacteria" id="ADZ09141">
        <property type="protein sequence ID" value="ADZ09141"/>
        <property type="gene ID" value="Metbo_0892"/>
    </dbReference>
    <dbReference type="GeneID" id="10277341"/>
    <dbReference type="KEGG" id="mel:Metbo_0892"/>
    <dbReference type="eggNOG" id="arCOG01383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_4_1_2"/>
    <dbReference type="OMA" id="HYRSAST"/>
    <dbReference type="OrthoDB" id="74894at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ09141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="355" mass="41155" checksum="8244DAD4A5BA105F" modified="2011-05-03" version="1">MDTVLKENSAYPNVVFIVLNWNGWQDTLECLKSIDKVNYPNYFFILVDNASEDDSIERIHGYCNTPLDDKINKESDIYLKIKNLVEIDFEDLNNVKIDLNTSLNSNRLKNMIMIKNDQNYGFTGGNNIALDFAKDNLKPDYYLLLNNDTVVEPDFLKNMMDSVQKHDNVGFAGPKIYYYNPEEVSNLLSFTGGTINLNTSEPSPIGKDQIDTGQYEADKVVDYVEGSCMLVSSELAESVGFFNQDYFTYWEEIDWCIRGKKAGFNTIYTHKAKIWHKCYGSDIGAMSIYYMIRNRFLFMKTNETMLQQFTSILYYFCYYFWKILFSLAFIRRDKTKLGSFLDGTIDGIKNIKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBX4</accession>
    <name>F0TBX4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase U32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2097"/>
    <dbReference type="EnsemblBacteria" id="ADZ10316">
        <property type="protein sequence ID" value="ADZ10316"/>
        <property type="gene ID" value="Metbo_2097"/>
    </dbReference>
    <dbReference type="KEGG" id="mel:Metbo_2097"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011540_0_1_2"/>
    <dbReference type="OMA" id="YGGVSHF"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="415" mass="46212" checksum="C945AB1A51646978" modified="2011-05-03" version="1">MVKIVELLSPAKDRISLISAIKNGANSVYVGLDGYNMRANIANFSMEEISEAAALCHQRGVKLYVCINTSLSEVEVEKLKVNMPKLKDSGADAVIVSDLGAVKIARDSEMEVHMSVQANITNSESLKVLKELGVNRIILSRELPIQSIQEIAVNSPMELEVFVHGAMCVAVSGRCFLSSYMYNKSANCGECLQPCRKEWKLTSEDDEEFILGENNFSNTGNTSETRATTHILSPRDLCMVEHIPLLIESGVKVFKIEGRAKPADYVATVTKVYREAINEYEAGLWNTPEHEENVIRWLEELKNVFNRGFDTGFYFNTPKENSSSNEAKYKKKDVGEVVNYYKRVQAAEIRIWDDIDVGDALIIQGQTTGSIEQTVESIQIDGKPVKKGAKGQNVAVHVTDIVRPGDNVYKMVFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TBZ4</accession>
    <name>F0TBZ4_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uroporphyrin-III C-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.107</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Metbo_2117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AL-21</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.107" evidence="5"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645687.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2117"/>
    <dbReference type="EnsemblBacteria" id="ADZ10336">
        <property type="protein sequence ID" value="ADZ10336"/>
        <property type="gene ID" value="Metbo_2117"/>
    </dbReference>
    <dbReference type="GeneID" id="10278580"/>
    <dbReference type="KEGG" id="mel:Metbo_2117"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OMA" id="TRPEQEE"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADZ10336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="243" mass="26485" checksum="AF64822A7844D5E0" modified="2011-05-03" version="1">MVVYLVGAGPGDPDLITLKAVKALENADVVVYDRLANEEILKHAKSAEMIYVGKKAGEHYKKQPEINEILIEQGLKHDVVVRLKGGDPFVFGRGGEEMLALFEKGIDVEFIPGVTSAIGVPTSAGLPVTHRGVATSLTVVTGHEDPTKDNKQVKWDFKADTIIILMGVGLLEENTREIMKYKDPETPVCVIEKGTSPDQRIITGTLKNITENHINTPALVVIGHVVDIYKNALKIRGEDINEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>F0TC39</accession>
    <name>F0TC39_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Valine--tRNA ligase</fullName>
            <ecNumber evidence="6">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Valyl-tRNA synthetase</fullName>
            <shortName evidence="6">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">valS</name>
        <name evidence="10" type="ordered locus">Metbo_0943</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644541.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_0943"/>
    <dbReference type="EnsemblBacteria" id="ADZ09190">
        <property type="protein sequence ID" value="ADZ09190"/>
        <property type="gene ID" value="Metbo_0943"/>
    </dbReference>
    <dbReference type="GeneID" id="10277392"/>
    <dbReference type="KEGG" id="mel:Metbo_0943"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="8">
        <location>
            <begin position="18"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="9">
        <location>
            <begin position="610"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="6">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="6">
        <location>
            <begin position="529"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="887" mass="102260" checksum="3FE5963592BB8041" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-09-29" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE0</accession>
    <name>F0TCE0_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Metbo_2193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AL-21</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645758.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2193"/>
    <dbReference type="EnsemblBacteria" id="ADZ10407">
        <property type="protein sequence ID" value="ADZ10407"/>
        <property type="gene ID" value="Metbo_2193"/>
    </dbReference>
    <dbReference type="GeneID" id="10278656"/>
    <dbReference type="KEGG" id="mel:Metbo_2193"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_5_0_2"/>
    <dbReference type="OMA" id="VASKCDE"/>
    <dbReference type="OrthoDB" id="108307at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADZ10407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="106" mass="11830" checksum="9804DE790C3DA922" modified="2011-05-03" version="1">MKEEVFFSKGMKHVKEDYPDLYEASIQLNDVCYSGKTLDYKTQKLIAIGIAAAASDDRAVKKQILSGMAELEITRDEVVDVLRVVLLLAGKPAFTKGMNALYKIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCE5</accession>
    <name>F0TCE5_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein UCP006577</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645763.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2198"/>
    <dbReference type="EnsemblBacteria" id="ADZ10412">
        <property type="protein sequence ID" value="ADZ10412"/>
        <property type="gene ID" value="Metbo_2198"/>
    </dbReference>
    <dbReference type="GeneID" id="10278661"/>
    <dbReference type="KEGG" id="mel:Metbo_2198"/>
    <dbReference type="eggNOG" id="arCOG04844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159086_1_0_2"/>
    <dbReference type="OMA" id="TKPCYGT"/>
    <dbReference type="OrthoDB" id="100968at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012031">
        <property type="entry name" value="UCP006577"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08979">
        <property type="entry name" value="DUF1894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006577">
        <property type="entry name" value="UCP006577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="102" mass="11371" checksum="1A8C0EAA6FAC1884" modified="2011-05-03" version="1">MSFCLEQHLQQSEDYKILIKNTGFKEAKKVIEKHSPKKVYVNAGEKILGARIIGIPPIPIGINDDESKVTLPYTKPCHGTAVVELPLEKDEIDKIKSSAIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCH0</accession>
    <name>F0TCH0_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Metbo_1000</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AL-21</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="7 8">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ09247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013644598.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_1000"/>
    <dbReference type="EnsemblBacteria" id="ADZ09247">
        <property type="protein sequence ID" value="ADZ09247"/>
        <property type="gene ID" value="Metbo_1000"/>
    </dbReference>
    <dbReference type="GeneID" id="10277449"/>
    <dbReference type="KEGG" id="mel:Metbo_1000"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_3_3_2"/>
    <dbReference type="OMA" id="APKDMFE"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADZ09247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="53" mass="5862" checksum="12D19E50BEF6CECC" modified="2011-05-03" version="1">MKKYLCTACGYVYDPEEGDPDNGIDPGTAFEDLPDDWVCPLCGVGKELFEETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP8</accession>
    <name>F0TCP8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="1">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Metbo_2224</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AL-21</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EC" id="7.2.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645789.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2224"/>
    <dbReference type="EnsemblBacteria" id="ADZ10438">
        <property type="protein sequence ID" value="ADZ10438"/>
        <property type="gene ID" value="Metbo_2224"/>
    </dbReference>
    <dbReference type="GeneID" id="10278690"/>
    <dbReference type="KEGG" id="mel:Metbo_2224"/>
    <dbReference type="eggNOG" id="arCOG01578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_3_2"/>
    <dbReference type="OMA" id="GVHRMAK"/>
    <dbReference type="OrthoDB" id="17967at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="812"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="11">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADZ10438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="837" mass="91580" checksum="2BAAA0B04335443C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCP9</accession>
    <name>F0TCP9_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0146 protein Metbo_2225</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Metbo_2225</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AL-21</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">AL-21</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0146 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645790.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2225"/>
    <dbReference type="EnsemblBacteria" id="ADZ10439">
        <property type="protein sequence ID" value="ADZ10439"/>
        <property type="gene ID" value="Metbo_2225"/>
    </dbReference>
    <dbReference type="GeneID" id="10278691"/>
    <dbReference type="KEGG" id="mel:Metbo_2225"/>
    <dbReference type="eggNOG" id="arCOG04385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148458_0_0_2"/>
    <dbReference type="OMA" id="LIYSIRP"/>
    <dbReference type="OrthoDB" id="92832at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016725">
        <property type="entry name" value="UCP016725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADZ10439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15352" checksum="B0501F966376853C" modified="2011-05-03" version="1">MWKDFSEYIITNYKDASKIVEVGMGNFPRVALTLQEHLNMDIIMTDIKPYHPTVTVDDINDPDLEIYRGASLIYSIRPPEELQKPIKTVADTVGADIIIKPFLTESFLNDKKFKLINYKTAIFYKKCNNEMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ2</accession>
    <name>F0TCQ2_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="16">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">carB</name>
        <name evidence="20" type="ordered locus">Metbo_2228</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">AL-21</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">AL-21</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="16">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 16">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10442.1"/>
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        <property type="nucleotide sequence ID" value="NC_015216.1"/>
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    <dbReference type="STRING" id="877455.Metbo_2228"/>
    <dbReference type="EnsemblBacteria" id="ADZ10442">
        <property type="protein sequence ID" value="ADZ10442"/>
        <property type="gene ID" value="Metbo_2228"/>
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    <dbReference type="GeneID" id="10278694"/>
    <dbReference type="KEGG" id="mel:Metbo_2228"/>
    <dbReference type="eggNOG" id="arCOG01594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_2_2"/>
    <dbReference type="OMA" id="IEPAGIH"/>
    <dbReference type="OrthoDB" id="911at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
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        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
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    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 16" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 16" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="131"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="667"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="19">
        <location>
            <begin position="925"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="16">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="16">
        <location>
            <begin position="927"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="817"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="831"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ADZ10442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="1060" mass="116964" checksum="75D74CDB819F712C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCQ8</accession>
    <name>F0TCQ8_METLA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Metbo_2234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AL-21</strain>
        </source>
    </reference>
    <reference evidence="1 2" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
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                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
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                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">AL-21</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002551">
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Alpha_ANH_like_I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
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        <property type="entry name" value="QueC"/>
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    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
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        <property type="entry name" value="PTHR43169:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00268">
        <property type="entry name" value="TIGR00268"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADZ10448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="349" mass="38817" checksum="9592CC957EFD57D2" modified="2011-05-03" version="1">MEVETKLQNLKDYLRDKNILIAFSGGADSSLVAKIASEVSKESLAVTVDNGVMPQDFIANATKIAGELGIKHRVVEENYLNDPAFRSNPPNRCYICKTKMYTKLAEIAGENELEIMDGTNITDLLEDRPGIIVNYDLEIKSPLVMAGFTSDDVKEALNQLEIEYSPSTTCMATRISKNSEITPKKINRLKYAESLLKGLSGSKVVRVRDQEDMAILEVENIEKLLNLGVLNHLDSELKAVGFKRVTLDISGHGDTKKELVVYKPCKDEANKIMFETELPYSINIKQTCAELETIGTVKCSVEMGIAMLESDGRNVTVFKNGKLVARKVADQEDAEQLLIEVLPKIRRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>F0TCS8</accession>
    <name>F0TCS8_METLA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="11">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">alaS</name>
        <name evidence="13" type="ordered locus">Metbo_2254</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanobacterium lacus (strain AL-21)</name>
        <dbReference type="NCBI Taxonomy" id="877455"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanobacteria</taxon>
            <taxon>Methanobacteriales</taxon>
            <taxon>Methanobacteriaceae</taxon>
            <taxon>Methanobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2011-02" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of Methanobacterium sp. AL-21.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Lucas S."/>
                <person name="Copeland A."/>
                <person name="Lapidus A."/>
                <person name="Cheng J.-F."/>
                <person name="Goodwin L."/>
                <person name="Pitluck S."/>
                <person name="Chertkov O."/>
                <person name="Detter J.C."/>
                <person name="Han C."/>
                <person name="Tapia R."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Ivanova N."/>
                <person name="Mikhailova N."/>
                <person name="Pagani I."/>
                <person name="Cadillo-Quiroz H."/>
                <person name="Imachi H."/>
                <person name="Zinder S."/>
                <person name="Liu W."/>
                <person name="Woyke T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AL-21</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="journal article" date="2014" name="Int. J. Syst. Evol. Microbiol." volume="64" first="1473" last="1480">
            <title>Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands.</title>
            <authorList>
                <person name="Cadillo-Quiroz H."/>
                <person name="Brauer S.L."/>
                <person name="Goodson N."/>
                <person name="Yavitt J.B."/>
                <person name="Zinder S.H."/>
            </authorList>
            <dbReference type="PubMed" id="24449792"/>
            <dbReference type="DOI" id="10.1099/ijs.0.059964-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">AL-21</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="11"/>
    <dbReference type="EMBL" id="CP002551">
        <property type="protein sequence ID" value="ADZ10468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013645819.1">
        <property type="nucleotide sequence ID" value="NC_015216.1"/>
    </dbReference>
    <dbReference type="STRING" id="877455.Metbo_2254"/>
    <dbReference type="EnsemblBacteria" id="ADZ10468">
        <property type="protein sequence ID" value="ADZ10468"/>
        <property type="gene ID" value="Metbo_2254"/>
    </dbReference>
    <dbReference type="GeneID" id="10278720"/>
    <dbReference type="KEGG" id="mel:Metbo_2254"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="7896at2157"/>
    <dbReference type="Proteomes" id="UP000007490">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="12">
        <location>
            <begin position="51"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="595"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADZ10468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000007490"/>
        </source>
    </evidence>
    <sequence length="905" mass="101729" checksum="8EDD0754AB31EDE4" modified="2011-05-03" version="1"> </sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>